CN207911659U - A kind of device for being used for EMS directed mutagenesis, screening salt tolerant alfalfa - Google Patents
A kind of device for being used for EMS directed mutagenesis, screening salt tolerant alfalfa Download PDFInfo
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Abstract
The utility model discloses a kind of for EMS directed mutagenesis, screens the device of salt tolerant alfalfa, including breeding apparatus and support device；The support device includes water planting box and rectangular plastic support, and the rectangular plastic support is detachably secured to by iron hook and connecting rope on the water planting box；The breeding apparatus is 72 hole hole trays, and 72 hole hole tray is fixed on by rectangular plastic support on water planting box, and hole tray bottom and the liquid level distance of institute's holding liquid in water planting box can be adjusted by connecting rope.The device can simulate the field water salt characteristics of motion, and the field moisture-salt transport simulated environment of emulation is provided for the screening of salt tolerant alfalfa, improves the success rate of salt tolerant alfalfa targeted mutagenesis screening.
Description
Technical field
The utility model is related to field of plant variety breeding technology, and in particular to one kind is purple for EMS directed mutagenesis, screening salt tolerant
The device of russian fenugreek herb.
Background technology
Soil salinization problem is worldwide widely present, and is the abiotic stress for the most serious that agricultural production faces
One of, seriously affect crop growth；There are sunlight-heat reaources to wait natural talents plentifully in salt-soda soil simultaneously, it would be highly desirable to exploitation and profit
With.
Alfalfa (Medicago sativa L.) is leguminous perennial herb, adaptable strong, grass yield
High, the advantages that palatability is good, is the maximum herbage of cultivated area in the world, is known as " King of Pasture ", has certain salt tolerant
Property；But salt damage is still limitation and one of determines clover an important factor for planting extensively, and it is resistance to further increase clover in varieties in saline-alkali areas
Salt is very necessary.The stronger alfalfa variety of salt tolerance is cultivated, is the material base that clover utilizes in salt-soda soil.
Selection and breeding for the strong alfalfa new varieties of salt tolerance mainly use traditional crossbreeding or gene work at present
Journey breeding.But traditional crossbreeding selection and breeding periodicity length, time and effort consuming.Genetic engineering breeding is still the ring based on manual simulation
Border lacks corresponding kind region attribute testing research；In addition, since the salt tolerance of plant is a number by controlled by multiple genes
Character is measured, and current research report pertains only to monogenic conversion mostly, this may be repressor gene engineering salt tolerant breeding hair
One of the reason of exhibition；Furthermore current research report is mostly to be commented in the salt tolerance of the growth early stage progress of transgenic strain (kind)
Analysis, and whether its salt tolerance can maintain and stabilize heredity and still remain to be confirmed, and the salt tolerance of some stage of development still cannot generation
The salt tolerance of table plant.
Chemical mutation has the characteristics that easy to use, mutation rate is high, offspring is easier to stablize heredity, is current crops
More common one of the effective means of breeding.In numerous chemical mutagens, ethylmethane sulfonate (EMS) is common efficient chemical
Mutagens have the characteristics that mutation type is wide, the frequency of mutation is high, mutation efficiency is high.But EMS mutagenesis has multidirectional,
Mutagenesis character can not be oriented and be predicted.Therefore, there has been no the reports about EMS directed mutagenesis, screening salt tolerant alfalfa at present.
Utility model content
In view of the above shortcomings of the prior art, the purpose of this utility model is to provide a kind of EMS directed mutagenesis, screening salt tolerant
The method of alfalfa.
The another object of the utility model is to provide the device for EMS directed mutagenesis, screening salt tolerant alfalfa.
To achieve the above object, the utility model adopts the following technical solution：
The utility model in a first aspect, a kind of method for providing EMS directed mutagenesis, screening salt tolerant alfalfa, including
Following steps：
(1) saliferous EMS- phosphate buffers are used to handle alfalfa seed；
(2) by step (1) treated alfalfa seed is placed in germination box, Stress treatment is carried out with NaCl solution,
Culture to seed is sprouted in illumination box；
(3) alfalfa seed that step (2) is sprouted is seeded in seedling screening device, and it is dense to step up salt stress
Degree screens Salt Tolerance at Seedling Stage single plant；
(4) the Salt Tolerance at Seedling Stage single plant that step (3) is screened is transplanted into crop field, growth period reinforces fertilizer and water management, and carries out
Character observation；
(5) excellent variation single plant is picked out, alfalfa seed is collected；
(6) alfalfa seed of step (5) is placed in germination box, Stress treatment is carried out with NaCl solution, in illumination
Culture to seed is sprouted in incubator；
(7) alfalfa seed that step (6) is sprouted is seeded in seedling screening device, and it is dense to step up salt stress
Degree screens Salt Tolerance at Seedling Stage single plant；
(8) divide single plant to sow in big field the Salt Tolerance at Seedling Stage single plant that step (7) is screened, that is, obtain alfalfa
EMS orients salt tolerant mutagenesis idioplasm.
Preferably, in step (1), in the saliferous EMS- phosphate buffers, the mass percentage of EMS is 1.0%,
NaCl concentration is 50mmol/L.
Preferably, in step (1), the processing method of alfalfa seed is：First by alfalfa seed with 10% H2O2
It disinfects, distilled water flushing is used after disinfecting, then soaked seed at room temperature to Expanded state, remove seed surface moisture, so
3-5h is handled at 4 DEG C with saliferous EMS- phosphate buffers afterwards, handles 3-5h at room temperature；After treatment outwells saliferous EMS-
Phosphate buffer, by treated, alfalfa seed flowing water rinses 1h or more.
Preferably, in step (2) and step (6), Stress treatment is carried out using the NaCl solution of 100mmol/L.
Preferably, in step (2) and step (6), 25 DEG C of daytime is kept in illumination box, at 20 DEG C of night, is become
Temperature culture to seed is sprouted.
Preferably, in step (3) and step (7), the seedling screening device includes：Breeding apparatus and support device；Institute
It includes water planting box and rectangular plastic support to state support device, and the rectangular plastic support is removably solid by iron hook and connecting rope
Due on the water planting box；The breeding apparatus is 72 hole hole trays, and 72 hole hole tray is fixed on water by rectangular plastic support
It trains on box, hole tray bottom and the liquid level distance of institute's holding liquid in water planting box can be adjusted by connecting rope.
Preferably, in step (3) and step (7), the method that uses of screening Salt Tolerance at Seedling Stage single plant for：By the pale reddish brown lucerne of sprouting
Mu seed is seeded in the hole tray of seedling screening device, and each cave moves one plant, and depth of planting is 0.5-1 centimetres, numbers, educates by strain
Ensure that moisture is sufficient between seedling stage；The concentration of NaCl and liquid level carry out seedling stage in water planting box by controlling seedling screening device
Salt Tolerance；First with clear water culture 7 days, Solution culture method is then changed to 30 days, is gradually added in nutrient solution later
NaCl to 50,100,150 and 200mmol/L are co-cultured 30 days or so in salting liquid, until clover survival rate is in 45- in hole tray
55%.
The field water salt characteristics of motion is simulated by the height of regulating liquid surface, wherein was flooded matrix 1h with liquid level, and was simulated
Irrigation process；Loaded filter was flooded with liquid level, simulates field high water level environment after just pouring, liquid level is left with hole tray and simulates moisture
Reduce supply environment.
Preferably, in step (4), the character observation includes：Albefaction, dwarfing, yellow, lopsided seedling, abnormal leaf, plant height,
Branch amount, compound leaf number, leaf blade size, growth potential and the speed of growth.
Preferably, in step (5), excellent variation single plant standard：Color is dark green, and growth potential is strong, and blade is loose, stem/leaf ratio
Small, branch is more, and the speed of growth is very fast, without bad characters such as albefaction, yellow, lopsided seedling, abnormal leafs.
The second aspect of the utility model provides a kind of device for being used for EMS directed mutagenesis, screening salt tolerant alfalfa,
Described device includes：Breeding apparatus and support device；The support device includes water planting box and rectangular plastic support, the rectangle
Plastic stent is detachably secured to by iron hook and connecting rope on the water planting box；The breeding apparatus is 72 hole hole trays, institute
72 hole hole trays are stated to be fixed on water planting box by rectangular plastic support, in hole tray bottom and water planting box the liquid level of institute's holding liquid away from
From can be adjusted by connecting rope.
Preferably, sponge is placed as loaded filter in each hole tray bottom of 72 hole hole tray, and loaded filter top is matrix
Layer.
The beneficial effects of the utility model：
(1) the EMS directed mutagenesis of the utility model, screen salt tolerant alfalfa method is easy to operate, efficient stable, at
This is cheap.This method can be utilized in production, in conjunction with the identification of simple economical character, can be oriented and be formulated and filter out salt tolerant purple
Russian fenugreek herb variation individual and group provide abundant germplasm materials for heredity, the breeding of alfalfa.
(2) device for being used for EMS directed mutagenesis, screening salt tolerant alfalfa designed by the utility model, can simulate
The field water salt characteristics of motion provides the field moisture-salt transport simulated environment of emulation for the screening of salt tolerant alfalfa, improves resistance to
The success rate of salt alfalfa targeted mutagenesis screening.
Description of the drawings
Fig. 1：The seedling screening apparatus structure schematic diagram of the utility model；Wherein, 1- water plantings box, 2- iron hooks, the modeling of 3- rectangles
Expect holder, the holes 4-72 hole tray, 5- connecting ropes.
Fig. 2：Hole tray structural schematic diagram；Wherein, 6- hypothallus, 7- loaded filters.
Specific implementation mode
It is noted that following detailed description is all illustrative, it is intended to provide further instruction to the application.Unless another
It indicates, all technical and scientific terms used herein has usual with the application person of an ordinary skill in the technical field
The identical meanings of understanding.
As described in background technology, due to EMS mutagenesis have multidirectional, mutagenesis character can not orient in advance
It surveys.In order to solve this technical barrier, the utility model has been put forward for the first time a kind of EMS directed mutagenesis, sieve through performing creative labour
The method for selecting salt tolerant alfalfa.
In a kind of embodiment of the utility model, given EMS directed mutagenesis, the side for screening salt tolerant alfalfa
Method includes the following steps：
(1) seed preparation is prepared with saliferous EMS- phosphate buffers：Full seed alfalfa seed is chosen, and by setting
Amount weighs the seed, prepares phosphate buffer solution (pH=7.0), and ethylmethane sulfonate (EMS) and NaCl are dissolved in the phosphoric acid
In buffer solution, saliferous EMS- phosphate buffers are made；In the saliferous EMS- phosphate buffers, the mass percentage of EMS is
1.0%, NaCl concentration 50mmol/L；
(2) seed treatment：Directed mutagenesis alfalfa seed is handled with the EMS- phosphate buffers of saliferous, obtains M1Generation；
(3) Salt Tolerance of germination period：By the alfalfa seed (M of the acquisition handled through EMS1) it is placed in germination box
It is interior, Stress treatment is carried out with NaCl solution, culture to seed sprouts (showing money or valuables one carries unintentionally) in illumination box；
(4) Salt Tolerance of seedling stage：The alfalfa seed of sprouting is seeded in the hole tray of seedling screening device, point
It does not number, salt stress concentration is stepped up in the seedling screening device of design, screen Salt Tolerance at Seedling Stage single plant；
(5) field planting and character observation：The alfalfa screened through hole tray is moved into crop field, growth period reinforces rich water
Management；Mainly character observation is carried out from the following aspect：Albefaction is downgraded, yellow, lopsided seedling, abnormal leaf, plant height, branch amount, compound leaf
Number, leaf blade size, growth potential, speed of growth etc..
(6) sowing：Pick out excellent variation single plant, excellent variation single plant standard：Color is dark green, and growth potential is strong, blade fertilizer
Greatly, stem/leaf ratio is small, and branch is more, and the speed of growth is very fast, without bad characters such as albefaction, yellow, lopsided seedling, abnormal leafs, collects excellent
Good variation single plant alfalfa seed (M2), and it is spare to retain choiceness；
(7) the secondary Salt Tolerance of germination period：By alfalfa seed (M2) be placed in germination box, with NaCl solution to seed
It is handled, and culture to seed sprouts (showing money or valuables one carries unintentionally) in illumination box.
(8) seedling stage secondary Salt Tolerance：To the alfalfa seed (M of sprouting2) it is seeded in the hole tray of seedling screening device
In, it numbers respectively, salt stress concentration is stepped up in the seedling screening device of design, screen Salt Tolerance at Seedling Stage single plant；
(9) by M2Alfalfa seedling of the generation through screening divides single plant to be sowed in big field.
Seedling stage is also specially devised in order to carry out Salt Tolerance of seedling stage and seedling stage secondary Salt Tolerance, the utility model
Screening plant, as shown in Figure 1, including breeding apparatus and support device；The support device includes water planting box 1 and rectangular plastic branch
Frame 3；The water planting box 1 is cuboid, and upper opening is placed with nutrient solution or clear water, the salinity of nutrient solution in water planting box 1
It can be adjusted；The rectangular plastic support 3 is located on the liquid level of the liquid held in the water planting box 1, passes through iron hook 2
It is detachably secured on the water planting box 1 with connecting rope 5；Wherein, iron hook 2 is arranged in 1 corresponding two long side of water planting box
On frame, suitable number of iron hook, such as 4 or 6 can be arranged as required to；Pass through company between iron hook 2 and rectangular plastic support 3
Splicing 5 connects, and the length of connecting rope 5 can be adjusted, in addition, the sectional area of rectangular plastic support 3 is less than cutting for water planting box 1
Area, to allow rectangular plastic support 3 to go deep into water planting box 1, and with held in water planting box 1 between the liquid level of liquid
Distance can be adjusted.
The breeding apparatus is preferably 72 hole hole trays 4, and 72 hole hole tray 4 is fixed on water planting by rectangular plastic support 3
On box 1；The bottom of 72 hole hole trays 4 and the liquid level distance of nutrient solution in water planting box 1 can be carried out by adjusting the length of connecting rope 5
It adjusts.
A round sponge is placed as loaded filter 7 in each cavities bottoms of 72 hole hole trays, and matrix is placed on loaded filter, as
Hypothallus 6 (Fig. 2).
Wherein, the nutrient solution placed in water planting box 1 at being grouped as preferably：
Four water-calcium nitrate 945mg/L, potassium nitrate 506mg/L, ammonium nitrate 80mg/L, potassium dihydrogen phosphate 136mg/L, magnesium sulfate
493mg/L, iron salt solutions 2.5ml/L, liquid microelement 5ml/L, pH=6.0；
The iron salt solutions at being grouped as preferably：
Ferrous sulfate heptahydrate 2.78g, disodium ethylene diamine tetraacetate (EDTA.Na) 3.73g, distilled water 500ml, pH=5.5；
The liquid microelement at being grouped as preferably：
Potassium iodide 0.83mg/L, boric acid 6.2mg/L, manganese sulfate 22.3mg/L, zinc sulfate 8.6mg/L, sodium molybdate 0.25mg/
L, copper sulphate 0.025mg/L, cobalt chloride 0.025mg/L.
It is used after above-mentioned solution is diluted 10 times.
The matrix placed in hole tray 4 is by turf, vermiculite and perlite in mass ratio 1:1:1 composition.
Above-mentioned EMS directed mutagenesis, screen salt tolerant alfalfa method in, each step complements each other, collaboration promote, be one
A organic whole.Since breeding is a long-term process, by pressurize selection can more efficiently selection and breeding to Objective
Shape, the utility model handle mutagenesis alfalfa seed using saliferous EMS- phosphate buffers, and one side seed is small in seed soaking 12
When after sprout related gene great expression, the expression of salt-resistant related gene can be stimulated at this time by being handled with salting liquid, and be lured with EMS
Change is coupled, and obtains preferable directed mutagenesis result；On the other hand, the salt treatment of low concentration (50mmol/L) sprouts alfalfa seed
Hair has facilitation, can be with partial offset germination percentage rapid decrease caused by EMS processing, it is easier to obtain mutant materials.
During the directed mutagenesis of salt tolerant alfalfa, screening, germination period and seedling stage are the key that two salt tolerant chosen periods, so
The utility model makes the characters of progenies of mutation breeding stablize using the two periods, the multiple screening technique of different generations.It is planting
In object mutation breeding, half lethal concentration is proper concentration for the treatment of, and survival rate is relatively low when being higher than this concentration, fine individual plant
Few, less than this concentration, then Mutagenic Effect is bad；The utility model is by the theoretical extension of half lethal concentration to salt tolerant alfalfa
In directed mutagenesis, therefore the survival rate of clover is set to 45-55%.To meet the preference temperature needed for Growth of Alfalfa,
When the low temperature seasons such as winter implement the utility model, the facilities such as greenhouse should be used, to provide the temperature condition needed for alfalfa growing.
More it is essential that coordinate the process of seedling stage Salt Tolerance and seedling stage secondary Salt Tolerance, this practicality new
Type has also been specifically designed seedling screening device, the concentration and liquid level of NaCl in the water planting box by controlling seedling screening device
Carry out Salt Tolerance at Seedling Stage screening.Wherein, the field water salt characteristics of motion is simulated by the height of regulating liquid surface.Wherein, it is flooded with liquid level
Matrix 1h is not crossed, irrigation process is simulated；Loaded filter was flooded with liquid level, simulates field high water level environment after just pouring；Use hole tray
It leaves liquid level simulation moisture and reduces supply environment, liquid level can be adjusted by controlling connecting rope length.When in water and nothing
When being cultivated in salt nutrient solution, every 7 days a cycles, wherein preceding 4 days liquid levels should be slightly above loaded filter or maintain an equal level, rear 3 days caves
Disk leaves liquid level, cultivates about January altogether.NaCl is gradually added in nutrient solution later, concentration is with 50,100,150 and 200mmol/L
Concentration gradient is incremented by.After various concentration NaCl is added in nutrient solution, liquid level must be risen to the position to maintain an equal level with matrix
It sets, time 1h.Liquid level is restored to the position to maintain an equal level with loaded filter after 1h, simulates field saline Irrigation process.Every 7 days
A cycle, wherein 4 days liquid levels should be slightly above loaded filter or maintain an equal level with loaded filter, hole tray leaves liquid level within 3 days, about trains altogether
It educates 30 days or so, until clover survival rate stops cultivating in 45-55% in hole tray.Therefore, the seedling screening of the utility model
Device can provide the field moisture-salt transport simulated environment of emulation for the screening of salt tolerant alfalfa, improve salt tolerant alfalfa
The success rate of targeted mutagenesis screening.
In order to enable those skilled in the art can clearly understand the technical solution of the application, below with reference to tool
The technical solution of the application is described in detail in the embodiment of body.
Test material used in the utility model embodiment is the test material of this field routine, can pass through business
Channel is commercially available.
Embodiment 1：
(1) seed preparation is prepared with saliferous EMS- phosphate buffers：Full seed alfalfa seed is chosen, and by setting
Amount weighs the seed, prepares the phosphate buffer solution (pH=7.0) of 0.1mol/L, and by NaCl and ethylmethane sulfonate (EMS)
It is dissolved in the phosphate buffer, saliferous EMS- phosphate buffers is made, wherein EMS mass percents are calculated as 1.0%, NaCl concentration
For 50mmol/L.The preparation method of the 0.1mol/L phosphate buffers of pH=7.0 is：Mother liquor A is the phosphoric acid hydrogen two of 0.2mol/L
Sodium solution；Mother liquor B is the sodium dihydrogen phosphate of 0.2mol/L.61ml mother liquor A and 39ml mother liquors B is taken to mix and be settled to
200ml。
(2) seed treatment：Mutagenesis alfalfa seed is handled with saliferous EMS- phosphate buffers, obtains M1 generations.Processing kind
Son method be：First by alfalfa seed with 10% H2O2Solution disinfection processing 10min, distilled water flushing 2 times, at room temperature
Seed soaking reaches Expanded state in 12 hours.Surface moisture is removed with filter paper, is then handled 4 hours at 4 DEG C with saliferous EMS solution, room
The lower processing of temperature 4 hours, period jog concussion during processing；After EMS solution treatments, EMS solution is outwelled, it will treated kind
Son rinses 1h or more with flowing water.The reason of mutagenesis alfalfa seed being handled with saliferous EMS- phosphate buffers, first, seed exists
Seed soaking sprouts related gene great expression after 12 hours, handles the expression that can stimulate salt-resistant related gene with salting liquid at this time,
And be coupled with EMS mutagenesis, obtain preferable directed mutagenesis result；Second is that the salt treatment of low concentration (50mmol/L) is to clover kind
Son, which is sprouted, facilitation, can be with partial offset germination percentage rapid decrease caused by EMS processing, it is easier to obtain mutagenesis material
Material.
(3) Salt Tolerance of germination period：The alfalfa seed (M1) of the acquisition handled through EMS is placed in germination box
It is interior, Stress treatment is carried out with NaCl solution, culture to seed sprouts (showing money or valuables one carries unintentionally) in illumination box.Specific method is：It will place
The seed managed carries out Stress treatment with 100mmol/L NaCl solutions, is oriented selection；By treated, seed is placed in hair
It carries out in bud box and in the incubator alternating temperature (daytime 25 DEG C/night 20 DEG C) culture and, to Seed sprouting, during which ensures filter paper moistening daily.
(4) Salt Tolerance of seedling stage：Seedling screening device is designed, the Salt Tolerance in seedling stage, seedling screening device are used for
Including breeding apparatus and support device, the support device includes water planting box 1 and rectangular plastic support 3, the rectangular plastic branch
Frame 3 is located on the liquid level of the nutrient solution in the water planting box, and the water is detachably secured to by iron hook 2 and connecting rope 5
It trains on box 1, nutrient solution or clear water is placed in water planting box 1, nutrient solution salinity is adjustable；The breeding apparatus is the hole tray 4 in 72 holes,
The hole tray 4 is fixed on by rectangular plastic support 3 on water planting box 1.The liquid level of 4 bottom of hole tray and nutrient solution in water planting box 1
Distance can be adjusted by connecting rope 5.A round sponge is placed as loaded filter 7 in each cavities bottom, is placed on loaded filter
Matrix, as hypothallus 6.
Seedling screening method is：By in the hole tray of the sprigging sprouted to seedling screening device, each cave moves one plant, broadcasts
Kind depth is 0.5-1 centimetres, is numbered by strain, ensures that moisture is sufficient during nursery.By the water planting box for controlling seedling screening device
The concentration and liquid level of interior NaCl carries out Salt Tolerance at Seedling Stage screening and is then changed to pancebrin culture first with clear water culture 7 days
To 30 days, NaCl to 50,100,150 and 200mmol/L is gradually added in nutrient solution later, is co-cultured 30 days in salting liquid
Left and right, until clover survival rate is in 45-55% in hole tray.Specially：Field moisture-salt transport is simulated by the height of regulating liquid surface
Rule.Wherein, matrix 1h was flooded with liquid level, and simulated irrigation process；Loaded filter was flooded with liquid level, simulates field after just pouring
High water level environment；Liquid level simulation moisture, which is left, with hole tray reduces supply environment.Liquid level can by control connecting rope length come
It adjusts.When in water with being cultivated in nutrient solution, every 7 days a cycles, wherein preceding 4 days liquid levels should be slightly above loaded filter or
Maintain an equal level, rear 3 days hole trays leave liquid level, cultivate about January altogether.Gradually be added NaCl in nutrient solution later, concentration with 50,100,
150 and 200mmol/L concentration gradients are incremented by.After various concentration NaCl is added in nutrient solution, liquid level must be risen to
The position to maintain an equal level with matrix, time 1h.Liquid level is restored to the position to maintain an equal level with loaded filter after 1h, simulates field brine
Irrigation process.Every 7 days a cycles, wherein 4 days liquid levels should be slightly above loaded filter or maintain an equal level, hole tray leaves liquid level within 3 days, altogether
It about cultivates 30 days or so, until clover survival rate stops cultivating in 45-55% in hole tray.
(5) field planting and character observation：The alfalfa screened through hole tray is moved into crop field, growth period reinforces rich water
Management；Character observation mainly carries out from the following aspect：Albefaction is downgraded, yellow, lopsided seedling, abnormal leaf, plant height, branch amount, multiple
The number of sheets, leaf blade size, growth potential, speed of growth etc..
(6) sowing：Pick out excellent variation single plant, excellent variation single plant standard：Color is dark green, and growth potential is strong, blade fertilizer
Greatly, stem/leaf ratio is small, and branch is more, and the speed of growth is very fast, without bad characters such as albefaction, yellow, lopsided seedling, abnormal leafs, collects purple
Russian fenugreek herb seed (M2), and it is spare to retain choiceness；
(7) the secondary Salt Tolerance of germination period：Alfalfa seed (M2) is placed in germination box, with NaCl solution to seed
It is handled, and culture to seed sprouts (showing money or valuables one carries unintentionally) in illumination box.Specific method is the same as (3).
(8) seedling stage secondary Salt Tolerance：The alfalfa seed (M2) of sprouting is seeded in the hole tray containing matrix, respectively
Number, steps up salt stress concentration in the dedicated unit of design, and screening alfalfa EMS orients salt tolerant mutagenesis idioplasm.Tool
Body method is the same as (4).
(9) single plant is divided to sow in big field alfalfa seedling of the M2 generations through screening.
The foregoing is merely the preferred embodiments of the application, are not intended to limit this application, for the skill of this field
For art personnel, the application can have various modifications and variations.Within the spirit and principles of this application, any made by repair
Change, equivalent replacement, improvement etc., should be included within the protection domain of the application.
Claims (2)
1. a kind of for EMS directed mutagenesis, the device of screening salt tolerant alfalfa, which is characterized in that described device：Including cultivating
Device and support device；The support device includes water planting box and rectangular plastic support, and the rectangular plastic support passes through iron hook
It is detachably secured on the water planting box with connecting rope；The breeding apparatus is 72 hole hole trays, and 72 hole hole tray passes through square
Shape plastic stent is fixed on water planting box, in hole tray bottom and water planting box the liquid level distance of institute's holding liquid can by connecting rope into
Row is adjusted.
2. the apparatus according to claim 1, which is characterized in that place sponge and make in each hole tray bottom of 72 hole hole tray
For loaded filter, loaded filter top is hypothallus.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201820278682.6U CN207911659U (en) | 2018-02-28 | 2018-02-28 | A kind of device for being used for EMS directed mutagenesis, screening salt tolerant alfalfa |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110521597A (en) * | 2019-09-06 | 2019-12-03 | 青岛农业大学 | The method of bleomycin mutagenesis alfalfa initiative Salinity tolerance germplasm |
-
2018
- 2018-02-28 CN CN201820278682.6U patent/CN207911659U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110521597A (en) * | 2019-09-06 | 2019-12-03 | 青岛农业大学 | The method of bleomycin mutagenesis alfalfa initiative Salinity tolerance germplasm |
| CN110521597B (en) * | 2019-09-06 | 2022-08-26 | 青岛农业大学 | Method for creating salt-tolerant germplasm by mutagenesis of alfalfa by bleomycin |
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