CN108077263A - A kind of cotton low temperature conditioning agent and preparation method thereof and application method - Google Patents
A kind of cotton low temperature conditioning agent and preparation method thereof and application method Download PDFInfo
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- CN108077263A CN108077263A CN201711385588.7A CN201711385588A CN108077263A CN 108077263 A CN108077263 A CN 108077263A CN 201711385588 A CN201711385588 A CN 201711385588A CN 108077263 A CN108077263 A CN 108077263A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/22—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom rings with more than six members
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
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- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
- A01G2013/004—Liquid mulch
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Abstract
The present invention provides a kind of cotton low temperature conditioning agent and preparation method thereof and application method, 24 epi-brassinolides help to improve cotton seedling low temperature tolerance ability in cotton low temperature conditioning agent provided by the invention；Under the dissolution of ethyl alcohol, it is ensured that 24 epi-brassinolides and azone are completely dissolved in water, and then each component is contributed to be absorbed as a solution by cotton seedling.Azone plays the role of bleeding agent, it can promote absorption of the cotton leaf to the low temperature conditioning agent, so that the other components such as 24 epi-brassinolides are penetrated into cotton leaf, improve the drug effect of the low temperature conditioning agent jointly with tween and organosilicon, and then improve the low temperature resistant power of cotton seedling.Embodiment the result shows that, cotton low temperature conditioning agent provided by the invention can improve the low temperature tolerance ability of cotton in seedling stage, slow down or avoid injury of the low temperature to cotton seedling cell membrane, alleviate destruction of the low temperature to chlorophyll, and then cotton seedling is made to keep relatively high photosynthetic capacity under low temperature stress, so as to promote plant growth.
Description
Technical field
The invention belongs to cotton Chilling stress control technique field more particularly to a kind of cotton low temperature conditioning agent and its preparations
Method and application method.
Background technology
Since the 1990s mid-term, Northwest inland cotton region becomes national cotton advantage producing region.In recent years 60%
Output of cotton is come to Northwest inland cotton region, but Northwest inland frost-free period is short, is subject to early period damage to plants caused by sudden drop in temperature and frost damage.Cotton in seedling stage
Weak cotton seedling, growth retardation, wilting or yellow can be caused by meeting low temperature, easily form " grown-ups and children's seedling "；And low temperature can make cotton plant susceptible
Dye fungi and induce various diseases, cause crop field be short of seedling disconnected ridge even replay, cause serious economic loss.
The regulation and control of cotton Chilling stress are mostly realized using covering with ground sheeting plant cotton technology or raising seedling of cotton transplantation technique now.
Covering with ground sheeting plant cotton is a kind of by increasing ground temperature realization cotton planting early enough, the technology that early emergence, seedling stage heat.Often
Covering with ground sheeting plant cotton technology avoids cotton seedling from being subject to injury from low temperature, is put in cotton to increase cotton in seedling stage temperature as far as possible
Still overlay film to cotton late growth stage just takes off film after seedling, causes to take off the increase of film difficulty, broken residual mulch is trapped in field.Covering with ground sheeting
Inevitably cause serious " white pollution ", contaminated soil environment causes farmland quality to decline, influence crop yield and
Quality most causes the unsustainable property of agricultural production at last.
Raising seedling of cotton transplantation technique is a kind of in control environment nursery, is transplanted to big Tanaka's again after cotton seedling physically well develops
Mode can effectively avoid cotton in seedling stage from meeting with injury from low temperature while cotton development stage is extended.Traditional seedling transplanting technique
Using nutritive cube carry out initial stage nursery, but traditional nutrition bowl seedling transplanting there is recruitment it is more, manage cumbersome, labor intensity
Greatly, the problem of Rural Areas at Present labor shortage is not suitable for.Novel seedling raising transplantation technique substitutes conventional nutraceutical to integrate nursery base
Alms bowl, although solving the problems, such as that feeding block seedlings raising exists to a certain extent, Novel seedling raising transplants the with high content of technology of requirement,
And comprehensive nursery base is needed, cotton planting cost can be increased by a relatively large margin, the receipts of cotton grower will necessarily be reduced compared with live streaming
Enter.
The content of the invention
In view of this, it is an object of the invention to provide cotton low temperature conditioning agent and preparation method thereof and application method, sheets
Cotton in seedling stage can be effectively alleviated or avoided by injury from low temperature in the conditioning agent that invention provides, and is using process operation letter
It is single, the pollution to environment will not be caused, the increase of production cost will not be caused.
In order to realize foregoing invention purpose, the present invention provides following technical scheme：
The present invention provides a kind of cotton low temperature conditioning agent, including：24- epi-brassinolides, ethyl alcohol, azone, have tween
Machine silicon and water；The quality of the 24- epi-brassinolides, the volume of ethyl alcohol, the volume of tween, the volume of azone, organosilicon
Quality and the volume ratio of water are (0.05~0.5) mg：(0.5~2) mL：(0.8~1.5) mL：(0.5~2.5) mL：(0.28~
0.32)g：1000mL.
Preferably, the quality of the 24- epi-brassinolides, the volume of ethyl alcohol, the volume of tween, the volume of azone, have
The quality of machine silicon and the volume ratio of water are (0.1~0.45) mg：(1.0~1.5) mL：(1.0~1.2) mL：(1.0~2.0) mL：
0.30g：1000mL.
Preferably, the organosilicon is poly- trisiloxanes；
The tween is the one or more in polysorbas20, polysorbate40 and Tween 80.
The present invention provides the preparation methods of cotton low temperature conditioning agent described in above-mentioned technical proposal, comprise the following steps：
(1) azone is added in after the 24- epi-brassinolides being dissolved in ethyl alcohol, obtains mixed liquor；
(2) mixed liquor and the water mixing obtained the step (1), obtains dilution；
(3) dilution that the step (2) obtains with tween and organosilicon is mixed, obtains cotton low temperature conditioning agent.
The present invention also provides preparation sides described in cotton low temperature conditioning agent described in above-mentioned technical proposal or above-mentioned technical proposal
The application method for the cotton low temperature conditioning agent that method is prepared, comprising：12~36h before pre- cooling, by the cotton low temperature tune
Section agent is applied on cotton seedling leaf.
Preferably, the amount of application of the cotton low temperature conditioning agent is with soil where the cotton seedling of cotton low temperature conditioning agent to be administered
Area on the basis of count, the amount of application of the cotton low temperature conditioning agent is 450~600kg/hm2。
Preferably, when the pre- cooling extent is below 2 DEG C, the amount of application of the cotton low temperature conditioning agent is
[450kg/hm2,475kg/hm2]；
When the pre- cooling extent for (2 DEG C, 5 DEG C] when, the usage amount of the cotton low temperature conditioning agent is (475kg/hm2,
520kg/hm2]；
When the pre- cooling extent is (5 DEG C, 10 DEG C), the usage amount of the cotton low temperature conditioning agent is (520kg/
hm2,550kg/hm2]；
When the pre- cooling extent is 10 DEG C or more, the usage amount of the cotton low temperature conditioning agent is (550kg/hm2,
600kg/hm2]。
Preferably, the time of the application is before 8 a.m. or after at 5 points in afternoon.
Preferably, the method for application is flat spray.
The present invention provides a kind of cotton low temperature conditioning agent, including：24- epi-brassinolides, ethyl alcohol, azone, have tween
Machine silicon and water；The quality of the 24- epi-brassinolides, the volume of ethyl alcohol, the volume of tween, the volume of azone, organosilicon
Quality and the volume ratio of water are (0.05~0.5) mg：(0.5~2) mL：(0.8~1.5) mL：(0.5~2.5) mL：(0.28~
0.32)g：1000mL.In the present invention, 24- epi-brassinolides help to improve cotton seedling low temperature tolerance ability；In the dissolving of ethyl alcohol
Under effect, it is ensured that 24- epi-brassinolides and azone are completely dissolved in water, and then contribute to each component as a solution by
Cotton seedling absorbs.Also, azone plays the role of bleeding agent, can promote absorption of the cotton leaf to the low temperature conditioning agent so that 24-
The other components such as epi-brassinolide are penetrated into cotton leaf, improve the medicine of the low temperature conditioning agent jointly with organosilicon and tween
Effect, and then improve the low temperature resistant power of cotton seedling.Embodiment the result shows that, cotton low temperature conditioning agent provided by the invention can carry
The low temperature tolerance ability of high cotton in seedling stage slows down or avoids injury of the low temperature to cotton seedling cell membrane, alleviates low temperature and chlorophyll is broken
It is bad, and then cotton seedling is made to keep relatively high photosynthetic capacity under low temperature stress, so as to promote plant growth.
Description of the drawings
Fig. 1 is per day from April 5th, 2017 to April 15 for experiment station of the Chinese Academy of Agriculture Science and Technologys Cotton Research Institute base
Temperature variation curve.
Specific embodiment
The present invention provides a kind of cotton low temperature conditioning agent, including：24- epi-brassinolides, ethyl alcohol, azone, have tween
Machine silicon and water；The quality of the 24- epi-brassinolides, the volume of ethyl alcohol, the volume of tween, the volume of azone, organosilicon
Quality and the volume ratio of water are (0.05~0.5) mg：(0.5~2) mL：(0.8~1.5) mL：(0.5~2.5) mL：(0.28~
0.32)g：1000mL.
Cotton low temperature conditioning agent provided by the invention includes 24- epi-brassinolides.In the present invention, the 24- tables oil
Rape lactone helps to improve cotton seedling low temperature tolerance ability.The present invention is to the specific source of the 24- epi-brassinolides without spy
Different requirement, using commercial goods well-known to those skilled in the art.With the stereometer of water, contain in every 1000mL water
The quality of 24- epi-brassinolides is 0.05~0.5mg, further preferably 0.1~0.45mg, more preferably 0.2~
0.3mg。
Cotton low temperature conditioning agent provided by the invention includes ethyl alcohol；In the present invention, the ethyl alcohol is preferably absolute ethyl alcohol
Or the ethyl alcohol that mass concentration is 98%, it is most preferably absolute ethyl alcohol.In the present invention, the ethyl alcohol is played to 24- tables rape element
The dissolving of lactone and azone convenient for improving the dissolubility in cotton low temperature conditioning agent, and then ensures that 24- epi-brassinolides are made
It gives full play to, improves the low temperature tolerance ability of cotton in seedling stage, can slow down or low temperature is avoided to be injured caused by cotton seedling.The present invention
There is no particular/special requirement to the source of the ethyl alcohol, using commercial goods well-known to those skilled in the art.With the body of water
Product is counted, and the volume containing ethyl alcohol is 0.5~2mL in every 1000mL water, further preferably 1.0~1.5mL, and more preferably 0.2
~0.3mL.
Cotton low temperature conditioning agent provided by the invention includes organosilicon；In the present invention, the organosilicon is preferably poly- three
Siloxanes.The present invention does not have particular/special requirement to the source of the organosilicon, using commercially available business well-known to those skilled in the art
Product.In the present invention, the organosilicon plays the role of spreader-sticker, improves the cotton low temperature conditioning agent in cotton leaf
Developing power helps the cotton low temperature conditioning agent and is fully absorbed by cotton leaf.With the stereometer of water, contain in every 1000mL water
There is the quality of organosilicon for 0.28~0.32g, further preferably 0.29~0.30g.
Cotton low temperature conditioning agent provided by the invention includes tween；In the present invention, the tween is preferably polysorbas20, spits
One or more in temperature 40 and Tween 80.The present invention does not have particular/special requirement to the specific source of the tween, using this field
Commercial goods known to technical staff.In the present invention, the tween is as emulsifier so that other components can be
It is even to be scattered in solution.With the stereometer of water, the volume containing tween is 0.8~1.5mL in every 1000mL water, further preferably
For 1.0~1.2mL, more preferably 1.05~1.15mL.
Cotton low temperature conditioning agent provided by the invention includes azone.In the present invention, the azone plays the work of bleeding agent
With absorption of the cotton leaf to the low temperature conditioning agent can be promoted so that the other components such as 24- epi-brassinolides penetrate into cotton leaf
In piece, improve the drug effect of the low temperature conditioning agent jointly with tween and organosilicon, and then improve the low temperature resistant power of cotton seedling.The present invention
There is no particular/special requirement to the specific source of the azone, using commercial goods well-known to those skilled in the art.With water
Stereometer, per 1000mL water in the volume containing azone be 0.5~2.5mL, further preferably 1.0~2.0mL, more preferably
For 1.5~1.8mL.
The present invention also provides the preparation methods of cotton low temperature conditioning agent described in above-mentioned technical proposal, comprise the following steps：
(1) azone is added in after the 24- epi-brassinolides being dissolved in ethyl alcohol, obtains mixed liquor；
(2) mixed liquor and the water mixing obtained the step (1), obtains dilution；
(3) dilution that the step (2) obtains with tween and organosilicon is mixed, obtains cotton low temperature conditioning agent.
The present invention adds in azone after the 24- epi-brassinolides are dissolved in ethyl alcohol, obtains mixed liquor.The present invention is by institute
It states 24- epi-brassinolides and azone is first dissolved in ethyl alcohol, it is ensured that can subsequently be completely dissolved in water, as a solution
It is absorbed by cotton seedling.The 24- epi-brassinolides are preferably dissolved in ethyl alcohol by the present invention under agitation；The present invention is to institute
Stating stirring condition does not have particular/special requirement, and the dissolving of the 24- epi-brassinolides in ethanol can be promoted by being subject to.In the present invention
In, the azone preferably adds under agitation, and the present invention does not have particular/special requirement to the stirring condition, described in it can promote
Subject to the dissolving of azone in ethanol.
After obtaining mixed liquor, the present invention mixes the mixed liquor and water, obtains dilution.In the present invention, it is described mixed
The mixing for closing liquid and water preferably carries out under agitation；The time of the stirring is preferably 30s~60s.In the present invention, institute
The mixing for stating mixed liquor and water is preferably that water is added in mixed liquor；The present invention is using first by 24- epi-brassinolides and nitrogen
Ketone is dissolved in ethyl alcohol, then the mode mixed with water obtains dilution, helps to be dissolved in the 24- epi-brassinolides in ethyl alcohol
It is completely dissolved in azone in water, solubility is low in water for solution 24- epi-brassinolides and azone, and caused low temperature is adjusted
The problem of component is difficult to be absorbed by cotton seedling in agent.
After obtaining dilution, the present invention mixes the dilution with tween and organosilicon, obtains cotton low temperature conditioning agent.
In the present invention, the mixing of the dilution, tween and organosilicon is preferably and first mixes the tween and dilution, then by institute
Organosilicon is stated to be added in the dilution dissolved with tween.In the present invention, the mixing of the tween and dilution is preferably being stirred
It is carried out under the conditions of mixing；The time of the stirring is preferably 30s~60s.The present invention realizes tween by controlling the stirring condition
Dissolving in dilution, and can avoid generating a large amount of foams.In the present invention, the addition of the organosilicon is preferably being stirred
It is carried out under the conditions of mixing, the present invention does not have particular/special requirement to the stirring condition, can promote the dissolving of organosilicon.
In the cotton low temperature conditioning agent preparation process, the dosage of each component of the present invention is can obtain aforementioned techniques
Constituent mass described in scheme is than subject to desired cotton low temperature conditioning agent.
The present invention also provides preparation sides described in cotton low temperature conditioning agent described in above-mentioned technical proposal or above-mentioned technical proposal
The application method for the cotton low temperature conditioning agent that method is prepared, comprising：12~36h before pre- cooling, by the cotton low temperature tune
Section agent is applied on cotton seedling leaf.
The present invention 12~36h before pre- cooling, the cotton low temperature conditioning agent is applied on cotton seedling leaf；Preferably drop
15 before temperature~for 24 hours, further preferably 18~21h.In the present invention, the time of the application be preferably before 8 a.m. or
It it is more preferably 5 points~8 points of 5 points~8 points of the morning or afternoon after at 5 points in afternoon.The present invention selects suitable administration time,
It can prevent from when sunlight intensity is big reagent being caused to evaporate, reduce drug effect.In the present invention, the method for application is preferably flat
Spray, further preferably puts down spray in the tow sides of cotton seedling leaf.The present invention is not special to the specific embodiment of the application
It is required that the method for application using liquid preparation well-known to those skilled in the art on cotton leaf；Such as aircraft spray
Medicine or machinery spray.The present invention does not have particular/special requirement to the seedling stage of cotton seedling to be administered, and low temperature is improved with any required before cooling down in advance
The cotton seedling of resistance.
In the present invention, the amount of application of the cotton low temperature conditioning agent is with where the cotton seedling of cotton low temperature conditioning agent to be administered
It is counted on the basis of the area of soil, the amount of application of the cotton low temperature conditioning agent is preferably 450~600kg/hm2。
The present invention further preferably determines the amount of application of the cotton low temperature conditioning agent according to cooling extent, both can guarantee that cotton seedling resisted
The raising of low temperature ability, and can ensure that the efficient utilization of the cotton low temperature conditioning agent.In the present invention, when the pre- cooling width
When degree is below 2 DEG C, the amount of application of the cotton low temperature conditioning agent is [450kg/hm2,475kg/hm2]；When the pre- cooling width
Spend for (2 DEG C, 5 DEG C] when, the usage amount of the cotton low temperature conditioning agent is (475kg/hm2,520kg/hm2]；When the pre- cooling
When amplitude is (5 DEG C, 10 DEG C), the usage amount of the cotton low temperature conditioning agent is (520kg/hm2,550kg/hm2]；When described pre-
When cooling extent is 10 DEG C or more, the usage amount of the cotton low temperature conditioning agent is (550kg/hm2,600kg/hm2]。
24- epi-brassinolides help to improve cotton seedling low temperature tolerance ability in cotton low temperature conditioning agent provided by the invention；
Under the dissolution of ethyl alcohol, it is ensured that 24- epi-brassinolides and azone are completely dissolved in water, and then contribute to each component with
The form of solution is absorbed by cotton seedling.Also, azone plays the role of bleeding agent, can promote cotton leaf to the low temperature conditioning agent
It absorbs so that the other components such as 24- epi-brassinolides are penetrated into cotton leaf, and the low temperature is improved jointly with organosilicon and tween
The drug effect of conditioning agent, and then improve the low temperature resistant power of cotton seedling.
The low temperature resistant ability of cotton seedling can be effectively improved using cotton low temperature conditioning agent provided by the invention, is put in cotton
Mulch can be taken off in time after seedling, directly cotton low temperature conditioning agent of the present invention is applied according to preceding solution, without passing through ground
The damage for being protected from low temperature of film not only improves the rate of recovery of mulch, moreover it is possible to reduce the residual amount of mulch in arable land；It can also
Culturing and transplanting seedlings recruitment caused by avoiding by the way of the culturing and transplanting seedlings is more, management is cumbersome, labor intensity is big and input cost
It improves.
With reference to embodiment to provided by the present invention for cotton low temperature conditioning agent and preparation method thereof and application method
It is described in detail, but they cannot be interpreted as limiting the scope of the present invention.
Embodiment 1
Prepare cotton low temperature conditioning agent：
It weighs 2mL absolute ethyl alcohols and is put into spare in stirring container, then weigh 0.3mg24- epi-brassinolides, be slowly added to
In spare absolute ethyl alcohol, stirring 30s is completely dissolved it.
2.5mL azones are weighed, in the absolute ethyl alcohol for being slowly added to have dissolved 24- epi-brassinolides, continue to stir 60s,
It is completely dissolved, obtains mixed liquor.
It weighs in the mixed liquor that 1000mL water adds in, after stirring and evenly mixing, adds 1.5mL polysorbas20s, stir 30s,
More degree stirrings is avoided to generate a large amount of foams.
It weighs the poly- trisiloxanes of 0.32g to add in previous solu, cotton low temperature conditioning agent is obtained after stirring evenly.
Embodiment 2
Cotton low temperature conditioning agent is prepared in the way of embodiment 1, difference lies in the dosage of each raw material components is respectively：
The 24- epi-brassinolides of 0.05mg, 0.5mL absolute ethyl alcohols, 1mL azones, 1L water, 0.8mL polysorbas20s, poly- three silicon of 0.28g
Oxygen alkane.
Embodiment 3
Cotton low temperature conditioning agent is prepared in the way of embodiment 1, difference lies in the dosage of each raw material components is respectively：
The 24- epi-brassinolides of 0.1mg, 1mL absolute ethyl alcohols, 1mL azones, 1L water, 0.8mL polysorbas20s, poly- three silica of 0.28g
Alkane.
Embodiment 4
Cotton low temperature conditioning agent is prepared in the way of embodiment 1, difference lies in the dosage of each raw material components is respectively：
The 24- epi-brassinolides of 0.2mg, 1.5mL absolute ethyl alcohols, 1mL azones, 1L water, 0.8mL polysorbas20s, poly- three silica of 0.28g
Alkane.
Embodiment 5
Cotton low temperature conditioning agent is prepared in the way of embodiment 1, difference lies in the dosage of each raw material components is respectively：
The 24- epi-brassinolides of 0.5mg, 2mL absolute ethyl alcohols, 1mL azones, 1L water, 0.8mL polysorbas20s, poly- three silica of 0.28g
Alkane.
Embodiment 6
The application effect of the cotton low temperature conditioning agent of the preparation of embodiment 2~5 is tested：
It is tested in March, 2017~April in experiment station of the Chinese Academy of Agriculture Science and Technologys Cotton Research Institute base (Anyang in Henan province
County, 36 ° of 07 ' N, 114 ° of 22 ' E) it carries out.Wherein, the temperature Change in 5~15 April of this area is as shown in Figure 1.
Grind 28 as material to be tested with Shandong cotton, sowed in advance March 26 and covered with double mulch film, April 5 emergence after and
Shi Fangmiao.Using randomized complete-block design, 3 pieces of regions per district's groups are set, are convenient for 3 repetitions.
Cooling was sprayed the previous day, that is, April 7 by machinery, sprayed distilled water (CK) and embodiment respectively on cotton seedling blade face
2~5 cottons prepared adjust preparation, are uniformly sprayed on cotton leaf using flat spray mode, Field information dosage is：500kg/
hm2.After the low temperature stress of 3 days, the relative conductivity of cotton seedling leaf, chlorophyll content, photosynthetic was measured respectively April 11
Performance index, photosynthetic rate and plant weights index, test result are as shown in table 1.
The performance indicator of cotton seedling after the conditioning agent that application sprinkling CK and embodiment 2~5 are prepared respectively of table 1
From the data of table 1, the conditioning agent that the present invention prepares is sprayed before cooling on cotton seedling blade face, with application go from
The cotton seedling of sub- water is compareed, and is decreased using the relative conductivity of the cotton seedling leaf of the prepared conditioning agent of the present invention, table
The bright conditioning agent can mitigate injury of the low temperature to cotton seedling leaf cell membrane.The cotton seedling leaf of conditioning agent is prepared using the present invention
Chlorophyll content, photochemical properties index and Net Photosynthetic Rate increase, show that the conditioning agent can mitigate low temperature to cotton
Spend photosynthetic influence.The plant weights for the cotton seedling leaf for preparing conditioning agent using the present invention increased, and show the tune
Section agent can alleviate inhibition of the low temperature to cotton seedling growth and development.As it can be seen that cotton low temperature conditioning agent provided by the invention can improve
The low temperature tolerance ability of cotton in seedling stage slows down or avoids injury of the low temperature to cotton seedling cell membrane, alleviates destruction of the low temperature to chlorophyll,
And then cotton seedling is made to keep relatively high photosynthetic capacity under low temperature stress, so as to promote plant growth.
As seen from the above embodiment, cotton low temperature conditioning agent provided by the invention can improve the low temperature resistant energy of cotton in seedling stage
Power slows down or avoids injury of the low temperature to cotton seedling cell membrane, alleviates destruction of the low temperature to chlorophyll, and then cotton seedling is made to be coerced in low temperature
Compel the relatively high photosynthetic capacity of lower holding, so as to promote cotton growth.
The above is only the preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, various improvements and modifications may be made without departing from the principle of the present invention, these improvements and modifications also should
It is considered as protection scope of the present invention.
Claims (9)
1. a kind of cotton low temperature conditioning agent, including：24- epi-brassinolides, ethyl alcohol, tween, azone, organosilicon and water；It is described
The quality of 24- epi-brassinolides, the volume of ethyl alcohol, the volume of tween, the volume of azone, the volume of the quality of organosilicon and water
Than for (0.05~0.5) mg：(0.5~2) mL：(0.8~1.5) mL：(0.5~2.5) mL：(0.28~0.32) g：1000mL.
2. cotton low temperature conditioning agent according to claim 1, which is characterized in that the quality of the 24- epi-brassinolides,
The volume of ethyl alcohol, the volume of tween, the volume of azone, the volume ratio of the quality of organosilicon and water are (0.1~0.45) mg：(1.0
~1.5) mL：(1.0~1.2) mL：(1.0~2.0) mL：0.30g：1000mL.
3. cotton low temperature conditioning agent according to claim 1 or 2, which is characterized in that the organosilicon is poly- trisiloxanes；
The tween is the one or more in polysorbas20, polysorbate40 and Tween 80.
4. the preparation method of any one of claims 1 to 3 cotton low temperature conditioning agent, comprises the following steps：
(1) azone is added in after the 24- epi-brassinolides being dissolved in ethyl alcohol, obtains mixed liquor；
(2) mixed liquor and the water mixing obtained the step (1), obtains dilution；
(3) dilution that the step (2) obtains with tween and organosilicon is mixed, obtains cotton low temperature conditioning agent.
5. the cotton that preparation method described in any one of claims 1 to 3 cotton low temperature conditioning agent or claim 4 is prepared
The application method of flower low temperature conditioning agent, comprising：12~36h before pre- cooling, cotton seedling is applied to by the cotton low temperature conditioning agent
On blade.
6. application method according to claim 5, which is characterized in that the amount of application of the cotton low temperature conditioning agent is to wait to apply
It is counted on the basis of the area of soil where the cotton seedling of cotton low temperature conditioning agent, the amount of application of the cotton low temperature conditioning agent is 450
~600kg/hm2。
7. application method according to claim 6, which is characterized in that described when the pre- cooling extent is below 2 DEG C
The amount of application of cotton low temperature conditioning agent is [450kg/hm2,475kg/hm2]；
When the pre- cooling extent for (2 DEG C, 5 DEG C] when, the usage amount of the cotton low temperature conditioning agent is (475kg/hm2,
520kg/hm2]；
When the pre- cooling extent is (5 DEG C, 10 DEG C), the usage amount of the cotton low temperature conditioning agent is (520kg/hm2,
550kg/hm2]；
When the pre- cooling extent is 10 DEG C or more, the usage amount of the cotton low temperature conditioning agent is (550kg/hm2,600kg/
hm2]。
8. application method according to claim 5, which is characterized in that the time of the application for before 8 a.m. or under
After 5 points of noon.
9. application method according to claim 5, which is characterized in that the mode of the application is flat spray.
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