CN108026536A - 使用crispr核酸筛选细菌、古细菌、藻类和酵母的方法 - Google Patents

使用crispr核酸筛选细菌、古细菌、藻类和酵母的方法 Download PDF

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CN108026536A
CN108026536A CN201680030913.5A CN201680030913A CN108026536A CN 108026536 A CN108026536 A CN 108026536A CN 201680030913 A CN201680030913 A CN 201680030913A CN 108026536 A CN108026536 A CN 108026536A
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nucleic acid
genome
sequence
colony
repetition
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R·巴伦古
K·M·泽勒
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North Carolina State University
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North Carolina State University
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    • A—HUMAN NECESSITIES
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    • A01N37/46—N-acyl derivatives
    • A—HUMAN NECESSITIES
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    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
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    • C12N15/102—Mutagenizing nucleic acids
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    • C12N15/09—Recombinant DNA-technology
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1079—Screening libraries by altering the phenotype or phenotypic trait of the host
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
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    • C12N2320/00—Applications; Uses
    • C12N2320/10—Applications; Uses in screening processes
    • C12N2320/12—Applications; Uses in screening processes in functional genomics, i.e. for the determination of gene function

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  • Crystallography & Structural Chemistry (AREA)
  • Dentistry (AREA)
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  • Agronomy & Crop Science (AREA)
  • Environmental Sciences (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Virology (AREA)
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  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
CN201680030913.5A 2015-05-29 2016-05-27 使用crispr核酸筛选细菌、古细菌、藻类和酵母的方法 Pending CN108026536A (zh)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201562168355P 2015-05-29 2015-05-29
US62/168,355 2015-05-29
US201662296853P 2016-02-18 2016-02-18
US62/296,853 2016-02-18
PCT/US2016/034812 WO2016196361A1 (en) 2015-05-29 2016-05-27 Methods for screening bacteria, archaea, algae, and yeast using crispr nucleic acids

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CN108026536A true CN108026536A (zh) 2018-05-11

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US (3) US10136649B2 (https=)
EP (2) EP4039816A1 (https=)
JP (3) JP2018516563A (https=)
KR (2) KR20220139447A (https=)
CN (1) CN108026536A (https=)
AU (2) AU2016270649B2 (https=)
CA (1) CA2983023A1 (https=)
EA (1) EA201792663A1 (https=)
HK (1) HK1253712A1 (https=)
WO (1) WO2016196361A1 (https=)

Cited By (2)

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CN110791578A (zh) * 2019-11-28 2020-02-14 广州微远基因科技有限公司 用于百日咳博德特氏杆菌的crispr检测引物组及其用途
CN112080571A (zh) * 2020-09-22 2020-12-15 扬州大学 基于CRISPR-Cas12b系统的空肠弯曲菌检测试剂盒和方法

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