CN108012923A - A kind of cultural method of selenium-rich roxburgh anoectochilus terminal bud - Google Patents
A kind of cultural method of selenium-rich roxburgh anoectochilus terminal bud Download PDFInfo
- Publication number
- CN108012923A CN108012923A CN201711271405.9A CN201711271405A CN108012923A CN 108012923 A CN108012923 A CN 108012923A CN 201711271405 A CN201711271405 A CN 201711271405A CN 108012923 A CN108012923 A CN 108012923A
- Authority
- CN
- China
- Prior art keywords
- selenium
- rich
- terminal bud
- roxburgh anoectochilus
- anoectochilus terminal
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Developmental Biology & Embryology (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention discloses a kind of cultural method of selenium-rich roxburgh anoectochilus terminal bud, belong to roxburgh anoectochilus terminal bud technical field of cultivation, technical points comprise the following steps：(1) selenium-rich black tea extracting solution is prepared；(2) adventitious bud proliferation, breeds more than 6 times in two months；(3) strong seedling culture；(4) selection of land is cultivated；(5) planting-site is handled；(6) roxburgh anoectochilus terminal bud tissue culture rooted seedling is moved into hardening canopy, is then transplanted into forest land seedbed；(7) in roxburgh anoectochilus terminal bud growth period, diluted with selenium-rich black tea extracting solution and clear water, obtain dilution, uniformly spray a dilution to blade face and culture medium every other month；(8) according to existing roxburgh anoectochilus terminal bud conventional cultivation technical standard, relevant maintenance management and pest and disease damage protection is carried out, ensures roxburgh anoectochilus terminal bud normal growth；A kind of the present invention is intended to provide cultural method for the selenium-rich roxburgh anoectochilus terminal bud that cultural method science, cultivation survival rate are high and the roxburgh anoectochilus terminal bud selenium content that is cultivated is higher；Cultivation for roxburgh anoectochilus terminal bud.
Description
Technical field
The present invention relates to a kind of roxburgh anoectochilus terminal bud cultural method, more specifically, more particularly to a kind of cultivation of selenium-rich roxburgh anoectochilus terminal bud
Method.
Background technology
Roxburgh anoectochilus terminal bud is orchid family roxburgh anoectochilus terminal bud category herbaceous plant, has the effect for the treatment of all kinds of diseases and ailments in popular legend, is known as " king of medicine "
Laudatory title.And the basic reason for playing its " mystery " curative effect is the pharmacological action that inherent chemical composition plays human body.Except medicine
With outer, it is also a kind of indoor sight leaf treasure of great ornamental value.
With the continuous improvement of people's living standards, health care consciousness is also gradually strengthening, ultimately resulting in roxburgh anoectochilus terminal bud market needs
Expansion is asked, price rises violently year by year, and once supply falls short of demand for roxburgh anoectochilus terminal bud.Roxburgh anoectochilus terminal bud is all wild before the nineties in last century, due to
Roxburgh anoectochilus terminal bud wild resource is rare and medical value is high, plus the long-term a large amount of immoderate excavations of people, causes now domestic wild
Roxburgh anoectochilus terminal bud resource is almost withered exhausted.After artificial culture success in 2006, present more than 90% roxburgh anoectochilus terminal bud is all artificial growth.
2013, related industry development was even more to advance by leaps and bounds.The provinces such as Fujian, Zhejiang, Jiangxi carry out the source protection of roxburgh anoectochilus terminal bud kind and production in succession
Product development.
The survey found that there are some technical problem for the large-scale production of roxburgh anoectochilus terminal bud：First, during tissue-cultured seedling productive culture
Between it is longer, active constituent content is waited to improve；Second, artificial growth and the roxburgh anoectochilus terminal bud heavy metal of culture, the residual problem of agriculture are still deposited
In exceeded phenomenon.Research shows that selenium has facilitation to crop growth and yield, is because suitable selenium enters plant
Interior, peroxidase activity rise, enhances oxidation resistance in plant body, so as to improve the resistance and anti-aging of plant
Ability, ensure that normal nutrition growth and the reproductive growth of plant.The toxicity of selenium energy antagonism heavy metal is own early to be had been reported that, mainly
By being combined into insoluble complex with heavy metal, it is excreted from being absorbed, resist environmental pollution and plant is caused
Harm.Therefore, the research of the application aspect of exploitation selenium-rich roxburgh anoectochilus terminal bud is strengthened, to improving the yield of roxburgh anoectochilus terminal bud and solving roxburgh anoectochilus terminal bud
Heavy metal, the residual excessive problem of agriculture etc. have great significance, and have wide development prospect.
The content of the invention
It is an object of the invention in view of the above shortcomings of the prior art, there is provided a kind of cultural method science, cultivation survive
The cultural method for the selenium-rich roxburgh anoectochilus terminal bud that rate is high and the roxburgh anoectochilus terminal bud selenium content that is cultivated is higher.
The technical proposal of the invention is realized in this way：A kind of cultural method of selenium-rich roxburgh anoectochilus terminal bud, this method include following
Step：
(1) extracting solution is prepared：Selenium-rich black tea end or coarse tea are screened, pure clear water is added after crushing, extraction is filtered
To selenium-rich black tea extracting solution；
(2) adventitious bud proliferation：It is explant to choose the interlude stipes without terminal bud and long root, is inoculated in MS culture mediums
On, breed more than 6 times within two months；
(3) strong seedling culture：It is transplanted to 1/2MS basal mediums and adds the strong seedling culture base of selenium-rich black tea extracting solution formation
Upper cultivation is extremely emerged；
(4) selection of land is cultivated：Using the method for hayashishita imitating wild planting roxburgh anoectochilus terminal bud, the woods more than height above sea level 400m is selected
Ground gully side frescade, nearly broad-leaf forest or pin broad-leaf forest hand over mixed area, relative air humidity 70%, Chang Feng little or quiet wind, printing opacity
Spend for 25~35%, around there is water source, soil pH is 4.5~6.5；
(5) planting-site is handled：The forest land chosen clean and smooth, then by the seedbed fertile soil handled well and richness
Selenium soil, sawdust, selenium-rich tea dust make base-material and fully mix, as plant matrix；
(6) roxburgh anoectochilus terminal bud tissue culture rooted seedling is moved into hardening canopy, hardening culture is removed after 6 days~14 days, cleaned up, with killing
Bacterium water sterilization is spread out after 8~12 minutes, obtains roxburgh anoectochilus terminal bud seedling, is transplanted into forest land seedbed；
(7) it is 1 by volume with selenium-rich black tea extracting solution and clear water in roxburgh anoectochilus terminal bud growth period:2~4 dilutions, must dilute
Liquid, every other month uniformly sprays blade face and culture medium dilution；
(8) according to existing roxburgh anoectochilus terminal bud conventional cultivation technical standard, relevant maintenance management and pest and disease damage protection is carried out, is ensured
Roxburgh anoectochilus terminal bud normal growth.
In a kind of cultural method of above-mentioned selenium-rich roxburgh anoectochilus terminal bud：Step (1) is specially：
A) selenium-rich black tea end or the coarse tea of Se content >=1.0mg/kg are screened, and is crushed in pulverizer, it is red to obtain selenium-rich
Tea powder；
B) clear water is added into the selenium-rich tea powder after crushing, control solid-liquid ratio is heated to 80~85 DEG C in 80~90g/L,
When constant temperature stirring 1~2 is small, rotating speed 120r/min, then cooled to room temperature, filtered to obtain filtrate A；
C) clear water is continuously added into the filter residue of step b), control solid-liquid ratio is heated to 70~75 in 110~120g/L
DEG C, when constant temperature stirring 1~1.5 is small, rotating speed 90r/min, then cooled to room temperature, is obtained by filtration liquor B；
D) boiling water is added into the filter residue of step c), control solid-liquid ratio soaks 20~30min, then in 140~150g/L
Cooled to room temperature, is obtained by filtration liquor C；
E) filtrate A, liquor B and liquor C are uniformly mixed, up to selenium-rich black tea extracting solution.
In a kind of cultural method of above-mentioned selenium-rich roxburgh anoectochilus terminal bud：Step (2) is specially：Adventitious bud proliferation：Choose without top
The interlude stipes of bud and long root is explant, is inoculated on MS culture mediums, while the auxiliary of following concentration is added in culture medium
Help material：BA3.2~4.5mg/L, 6~8g/L of NAA0.5~1.0mg/L, 28~30g/L of sucrose and agar, breed 6 times in two months
More than.
In a kind of cultural method of above-mentioned selenium-rich roxburgh anoectochilus terminal bud：In step (2), Medium's PH Value is 5.2~5.8；Culture
Condition is：23~27 DEG C, 11~13h/d of light application time, 1500~2000lx of intensity of illumination of temperature.
In a kind of cultural method of above-mentioned selenium-rich roxburgh anoectochilus terminal bud：In strong seedling culture base described in step (3), also it is added with down
State the auxiliary material of concentration：27~30g/L of sucrose, NAA2.0~3.0mg/L and 95~100g/L of bananas juice.
In a kind of cultural method of above-mentioned selenium-rich roxburgh anoectochilus terminal bud：The addition of selenium-rich black tea extracting solution is dense described in step (3)
Spend for 100~110g/L.
In a kind of cultural method of above-mentioned selenium-rich roxburgh anoectochilus terminal bud：In step (5), the Se content of the selenium-rich soil for 0.5~
1.5mg/kg。
In a kind of cultural method of above-mentioned selenium-rich roxburgh anoectochilus terminal bud：In step (5), the forest land chosen clean and smooth
Specially：The weeds for selecting forest land and miscellaneous filling are removed, is opened up wasteland along contour direction, stepping type, by ribbon-like manner, forms seedbed,
With lime disinfection 2~4 days, every square metre was applied 0.05~0.1 kilogram.
In a kind of cultural method of above-mentioned selenium-rich roxburgh anoectochilus terminal bud：In step (5), seedbed fertile soil and selenium-rich soil, sawdust, richness
The weight ratio of selenium tea dust is 2~4：2~3：1~2：0.5~1.
In a kind of cultural method of above-mentioned selenium-rich roxburgh anoectochilus terminal bud：In the step (7), every other month to blade face and cultivation
When matrix uniformly sprays dilution, foliage-spray amount is 300~450ml/m2, 550~750ml/m of the matrix amount of spraying2。
After the present invention uses the above method, compared with prior art, there is following beneficial effects：
(1) use selenium-rich soil as cultivation matrix (Se content in selected selenium-rich soil for 1.0~1.5mg/kg,
National selenium-rich soil standard is 0.4mg/kg), meanwhile, selenium-rich black tea extracting solution is made, in the every month of roxburgh anoectochilus terminal bud growth period,
Spraying the extracting solution by several times on blade face, (the selenium-rich black tea end of raw material Se content >=1.0mg/kg or coarse tea, Se rich tea standard are
0.15mg/kg, the extracting solution Se content are 40-60 ng/kgs), effectively ensure the absorption of selenium in roxburgh anoectochilus terminal bud cultivation
Amount, practice have shown that, which is entirely capable of the standard for reaching selenium-rich roxburgh anoectochilus terminal bud, and Se content reaches 0.42mg/kg (selenium-rich gold threads
The standard 0.15mg/kg of lotus).
(2) selenium has facilitation to crop growth and yield, is the peroxide because suitable selenium enters in plant
Enzymatic activity raises, and enhances oxidation resistance in plant body, so as to improve the resistance and anti-aging ability of plant, ensure that
The normal growth of plant, substantially reduces the incidence of one of the most serious disease of roxburgh anoectochilus terminal bud anthracnose.
(3) it with the addition of the tea grounds after selenium-rich black tea end or extraction in matrix, because containing substantial amounts of nutriment in tealeaves, such as
Protein accounts for 20-30%, and tea polyphenols account for the 20-36% of dry matter, at the same time containing things such as carbohydrate, vitamin, amino acid
Matter etc., can supplement substantial amounts of nutriment in matrix, while selenium promotes the growth of roxburgh anoectochilus terminal bud and introduces the generation of pest and disease damage, institute
The selenium-rich roxburgh anoectochilus terminal bud stable quality and height of cultivation, yield lifting 12~15%；
(4) nutrient matrix is full of nutrition and the facilitation of selenium, the content of anoectochilus roxburghii polyose improve 15~18%；
(5) roxburgh anoectochilus terminal bud of the planting technology has Se content height, drug action clear advantage.
(6) selenium energy antagonism heavy metal, resists environmental pollution and is endangered caused by plant, substantially reduce the heavy metal of roxburgh anoectochilus terminal bud
Pollution level, reduces Pesticide use amount.
Embodiment
With reference to specific embodiment, the present invention is described in further detail, but does not form to any of the present invention
Limitation.
A kind of cultural method of selenium-rich roxburgh anoectochilus terminal bud of the present invention, this method comprise the following steps：
(1) extracting solution is prepared, is specially：
A) selenium-rich black tea end or the coarse tea of Se content >=1.0mg/kg are screened, and is crushed in pulverizer, it is red to obtain selenium-rich
Tea powder；
B) clear water is added into the selenium-rich tea powder after crushing, control solid-liquid ratio is heated to 80~85 DEG C in 80~90g/L,
When constant temperature stirring 1~2 is small, rotating speed 120r/min, then cooled to room temperature, filtered to obtain filtrate A；
C) clear water is continuously added into the filter residue of step b), control solid-liquid ratio is heated to 70~75 in 110~120g/L
DEG C, when constant temperature stirring 1~1.5 is small, rotating speed 90r/min, then cooled to room temperature, is obtained by filtration liquor B；
D) boiling water is added into the filter residue of step c), control solid-liquid ratio soaks 20~30min, then in 140~150g/L
Cooled to room temperature, is obtained by filtration liquor C；
E) filtrate A, liquor B and liquor C are uniformly mixed, up to selenium-rich black tea extracting solution, which is 0.4
~0.6mg/kg.
50~55% Organic Selenium and most of tealeaves nutrition composition in the liquid dissolving tealeaves as obtained by carrying multiple water,
Preparation process is simple, and cost is low, and what is worked to roxburgh anoectochilus terminal bud growth is not only organic selenium solution, because being sought in tealeaves containing substantial amounts of
Material is supported, as protein accounts for 20-30%, tea polyphenols account for the 20-36% of dry matter, at the same time containing carbohydrate, vitamin, ammonia
The materials such as base acid, can supplement substantial amounts of nutriment in matrix and be absorbed for roxburgh anoectochilus terminal bud, the growth to roxburgh anoectochilus terminal bud also has necessarily
Facilitation.
(2) adventitious bud proliferation：It is explant to choose the interlude stipes without terminal bud and long root, is inoculated in MS culture mediums
On, while the auxiliary material of following concentration is added in culture medium：BA3.2~4.5mg/L, NAA0.5~1.0mg/L, sucrose 28~
6~8g/L of 30g/L and agar, breeds more than 6 times in two months.Medium's PH Value is 5.2~5.8；Condition of culture is：Temperature 23~
27 DEG C, 11~13h/d of light application time, 1500~2000lx of intensity of illumination.Above-mentioned concentration, is that raw material accounts for final culture medium total amount
Concentration values.
(3) strong seedling culture：It is transplanted to 1/2MS basal mediums and adds the strong seedling culture base of selenium-rich black tea extracting solution formation
Upper cultivation is extremely emerged；In strong seedling culture base, the also auxiliary material added with following concentration：27~30g/L of sucrose, NAA2.0~
95~100g/L of 3.0mg/L and bananas juice.Meanwhile the addition concentration of selenium-rich black tea extracting solution is 100~110g/L.It is above-mentioned dense
Degree, is the concentration values that raw material accounts for final culture medium total amount.
(4) selection of land is cultivated：Using the method for hayashishita imitating wild planting roxburgh anoectochilus terminal bud, the woods more than height above sea level 400m is selected
Ground gully side frescade, nearly broad-leaf forest or pin broad-leaf forest hand over mixed area, relative air humidity 70%, Chang Feng little or quiet wind, printing opacity
Spend for 25~35%, around there is water source, soil pH is 4.5~6.5；
(5) planting-site is handled：Choose forest land, remove the weeds for selecting forest land and miscellaneous filling, along contour direction, stepping type,
Opened up wasteland by ribbon-like manner, form seedbed, with lime disinfection 2~4 days, every square metre was applied 0.05~0.1 kilogram；Then will processing
Good seedbed fertile soil makees base-material with selenium-rich soil, sawdust, selenium-rich tea dust and fully mixes, as plant matrix；Seedbed fertile soil with
Selenium-rich soil, sawdust, the weight ratio of selenium-rich tea dust are 2~4：2~3：1~2：0.5~1.Wherein the Se content of selenium-rich soil for 0.5~
1.5mg/kg。
(6) roxburgh anoectochilus terminal bud tissue culture rooted seedling is moved into hardening canopy, hardening culture is removed after 6 days~14 days, cleaned up, with killing
Bacterium water sterilization is spread out after 8~12 minutes, obtains roxburgh anoectochilus terminal bud seedling, is transplanted into forest land seedbed.
(7) it is 1 by volume with selenium-rich black tea extracting solution and clear water in roxburgh anoectochilus terminal bud growth period:2~4 dilutions, must dilute
Liquid, every other month uniformly sprays blade face and culture medium dilution；Foliage-spray amount is 300~450ml/m2, base
550~750ml/m of the matter amount of spraying2。
(8) according to existing roxburgh anoectochilus terminal bud conventional cultivation technical standard, relevant maintenance management and pest and disease damage protection is carried out, is ensured
Roxburgh anoectochilus terminal bud normal growth.
Embodiment 1
A kind of cultural method of selenium-rich roxburgh anoectochilus terminal bud, this method comprise the following steps：
(1) extracting solution is prepared, is specially：
A) selenium-rich black tea end or the coarse tea of Se content >=1.0mg/kg are screened, and is crushed in pulverizer, it is red to obtain selenium-rich
Tea powder；
B) clear water is added into the selenium-rich tea powder after crushing, it is 80g/L to control solid-liquid ratio, is heated to 85 DEG C, constant temperature stirring 1
Hour, rotating speed 120r/min, then cooled to room temperature, filtered to obtain filtrate A；
C) clear water is continuously added into the filter residue of step b), it is 110g/L to control solid-liquid ratio, is heated to 75 DEG C, constant temperature stirring
1 it is small when, rotating speed 90r/min, then cooled to room temperature, is obtained by filtration liquor B；
D) boiling water is added into the filter residue of step c), control solid-liquid ratio soaks 30min, then natural cooling in 140g/L
To room temperature, liquor C is obtained by filtration；
E) filtrate A, liquor B and liquor C are uniformly mixed, up to selenium-rich black tea extracting solution.
(2) adventitious bud proliferation：It is explant to choose the interlude stipes without terminal bud and long root, is inoculated in MS culture mediums
On, while the auxiliary material of following concentration is added in culture medium：BA3.2mg/L, NAA0.5mg/L, sucrose 28g/L and agar 6g/
L, breeds more than 6 times in two months.Medium's PH Value is 5.2；Condition of culture is：23 DEG C, light application time 11h/d of temperature, illumination is strong
Spend 1500lx.
(3) strong seedling culture：It is transplanted to 1/2MS basal mediums and adds the strong seedling culture base of selenium-rich black tea extracting solution formation
Upper cultivation is extremely emerged；In strong seedling culture base, the also auxiliary material added with following concentration：Sucrose 27g/L, NAA2.0mg/L and banana
Juice 95g/L.Meanwhile the addition concentration of selenium-rich black tea extracting solution is 100g/L.
(4) selection of land is cultivated：Using the method for hayashishita imitating wild planting roxburgh anoectochilus terminal bud, the woods more than height above sea level 400m is selected
Ground gully side frescade, nearly broad-leaf forest or pin broad-leaf forest hand over mixed area, relative air humidity 70%, Chang Feng little or quiet wind, printing opacity
Spend for 25%, around there is water source, soil pH 4.5；
(5) planting-site is handled：Choose forest land, remove the weeds for selecting forest land and miscellaneous filling, along contour direction, stepping type,
Opened up wasteland by ribbon-like manner, form seedbed, with lime disinfection 2 days, every square metre was applied 0.05 kilogram；Then the seedbed that will be handled well
Fertile soil makees base-material with selenium-rich soil, sawdust, selenium-rich tea dust and fully mixes, as plant matrix；Seedbed fertile soil and selenium-rich soil, wood
Bits, the weight ratio of selenium-rich tea dust are 2：2：1：0.5.Wherein the Se content of selenium-rich soil is 0.5mg/kg.
(6) roxburgh anoectochilus terminal bud tissue culture rooted seedling is moved into hardening canopy, hardening culture is removed after 6 days, cleaned up, disappeared with sterilized water
Poison is spread out after 8~12 minutes, is obtained roxburgh anoectochilus terminal bud seedling, is transplanted into forest land seedbed；
(7) it is 1 by volume with selenium-rich black tea extracting solution and clear water in roxburgh anoectochilus terminal bud growth period:2 dilutions, obtain dilution,
A dilution is uniformly sprayed to blade face and culture medium every other month；Foliage-spray amount is 300ml/m2, the matrix amount of spraying
550ml/m2。
(8) according to existing roxburgh anoectochilus terminal bud conventional cultivation technical standard, relevant maintenance management and pest and disease damage protection is carried out, is ensured
Roxburgh anoectochilus terminal bud normal growth.
Embodiment 2
A kind of cultural method of selenium-rich roxburgh anoectochilus terminal bud, this method comprise the following steps：
(1) extracting solution is prepared, is specially：
A) selenium-rich black tea end or the coarse tea of Se content >=1.0mg/kg are screened, and is crushed in pulverizer, it is red to obtain selenium-rich
Tea powder；
B) clear water is added into the selenium-rich tea powder after crushing, control solid-liquid ratio is heated to 80 DEG C, constant temperature stirring 2 in 90g/L
Hour, rotating speed 120r/min, then cooled to room temperature, filtered to obtain filtrate A；
C) clear water is continuously added into the filter residue of step b), control solid-liquid ratio is heated to 70 DEG C, constant temperature stirring in 120g/L
1.5 it is small when, rotating speed 90r/min, then cooled to room temperature, is obtained by filtration liquor B；
D) boiling water is added into the filter residue of step c), control solid-liquid ratio soaks 20min, then natural cooling in 150g/L
To room temperature, liquor C is obtained by filtration；
E) filtrate A, liquor B and liquor C are uniformly mixed, up to selenium-rich black tea extracting solution.
(2) adventitious bud proliferation：It is explant to choose the interlude stipes without terminal bud and long root, is inoculated in MS culture mediums
On, while the auxiliary material of following concentration is added in culture medium：BA4.5mg/L, NAA1.0mg/L, sucrose 30g/L and agar 8g/
L, breeds more than 6 times in two months.Medium's PH Value is 5.8；Condition of culture is：27 DEG C, light application time 13h/d of temperature, illumination is strong
Spend 2000lx.
(3) strong seedling culture：It is transplanted to 1/2MS basal mediums and adds the strong seedling culture base of selenium-rich black tea extracting solution formation
Upper cultivation is extremely emerged；In strong seedling culture base, the also auxiliary material added with following concentration：Sucrose 30g/L, NAA3.0mg/L and banana
Juice 100g/L.Meanwhile the addition concentration of selenium-rich black tea extracting solution is 110g/L.
(4) selection of land is cultivated：Using the method for hayashishita imitating wild planting roxburgh anoectochilus terminal bud, the woods more than height above sea level 400m is selected
Ground gully side frescade, nearly broad-leaf forest or pin broad-leaf forest hand over mixed area, relative air humidity 70%, Chang Feng little or quiet wind, printing opacity
Spend for 35%, around there is water source, soil pH 6.5；
(5) planting-site is handled：Choose forest land, remove the weeds for selecting forest land and miscellaneous filling, along contour direction, stepping type,
Opened up wasteland by ribbon-like manner, form seedbed, with lime disinfection 4 days, every square metre was applied 0.1 kilogram；Then the seedbed that will be handled well
Soil makees base-material with selenium-rich soil, sawdust, selenium-rich tea dust and fully mixes, as plant matrix；Seedbed fertile soil and selenium-rich soil, sawdust,
The weight ratio of selenium-rich tea dust is 3：2.5：1.5：0.8.Wherein the Se content of selenium-rich soil is 1mg/kg.
(6) roxburgh anoectochilus terminal bud tissue culture rooted seedling is moved into hardening canopy, hardening culture is removed after 14 days, cleaned up, and uses sterilized water
Disinfection is spread out after 12 minutes, is obtained roxburgh anoectochilus terminal bud seedling, is transplanted into forest land seedbed；
(7) it is 1 by volume with selenium-rich black tea extracting solution and clear water in roxburgh anoectochilus terminal bud growth period:4 dilutions, obtain dilution,
A dilution is uniformly sprayed to blade face and culture medium every other month；Foliage-spray amount is 450ml/m2, the matrix amount of spraying
750ml/m2。
(8) according to existing roxburgh anoectochilus terminal bud conventional cultivation technical standard, relevant maintenance management and pest and disease damage protection is carried out, is ensured
Roxburgh anoectochilus terminal bud normal growth.
Embodiment 3
A kind of cultural method of selenium-rich roxburgh anoectochilus terminal bud, this method comprise the following steps：
(1) extracting solution is prepared, is specially：
A) selenium-rich black tea end or the coarse tea of Se content >=1.0mg/kg are screened, and is crushed in pulverizer, it is red to obtain selenium-rich
Tea powder；
B) clear water is added into the selenium-rich tea powder after crushing, control solid-liquid ratio is heated to 82 DEG C, constant temperature stirring in 85g/L
1.5 it is small when, rotating speed 120r/min, then cooled to room temperature, filtered to obtain filtrate A；
C) clear water is continuously added into the filter residue of step b), control solid-liquid ratio is heated to 73 DEG C, constant temperature stirring in 115g/L
1.2 it is small when, rotating speed 90r/min, then cooled to room temperature, is obtained by filtration liquor B；
D) boiling water is added into the filter residue of step c), control solid-liquid ratio soaks 25min, then natural cooling in 145g/L
To room temperature, liquor C is obtained by filtration；
E) filtrate A, liquor B and liquor C are uniformly mixed, up to selenium-rich black tea extracting solution.
(2) adventitious bud proliferation：It is explant to choose the interlude stipes without terminal bud and long root, is inoculated in MS culture mediums
On, while the auxiliary material of following concentration is added in culture medium：BA3.8mg/L, NAA0.8mg/L, sucrose 29g/L and agar 7g/
L, breeds more than 6 times in two months.Medium's PH Value is 5.5；Condition of culture is：25 DEG C, light application time 12h/d of temperature, illumination is strong
Spend 1800lx.
(3) strong seedling culture：It is transplanted to 1/2MS basal mediums and adds the strong seedling culture base of selenium-rich black tea extracting solution formation
Upper cultivation is extremely emerged；In strong seedling culture base, the also auxiliary material added with following concentration：Sucrose 28g/L, NAA2.5mg/L and banana
Juice 98g/L.Meanwhile the addition concentration of selenium-rich black tea extracting solution is 105g/L.
(4) selection of land is cultivated：Using the method for hayashishita imitating wild planting roxburgh anoectochilus terminal bud, the woods more than height above sea level 400m is selected
Ground gully side frescade, nearly broad-leaf forest or pin broad-leaf forest hand over mixed area, relative air humidity 70%, Chang Feng little or quiet wind, printing opacity
Spend for 30%, around there is water source, soil pH 5；
(5) planting-site is handled：Choose forest land, remove the weeds for selecting forest land and miscellaneous filling, along contour direction, stepping type,
Opened up wasteland by ribbon-like manner, form seedbed, with lime disinfection 3 days, every square metre was applied 0.08 kilogram；Then the seedbed that will be handled well
Soil makees base-material with selenium-rich soil, sawdust, selenium-rich tea dust and fully mixes, as plant matrix；Seedbed fertile soil and selenium-rich soil, sawdust,
The weight ratio of selenium-rich tea dust is 4：3：2：1.Wherein the Se content of selenium-rich soil is 1.5mg/kg.
(6) roxburgh anoectochilus terminal bud tissue culture rooted seedling is moved into hardening canopy, hardening culture is removed after 10 days, cleaned up, and uses sterilized water
Disinfection is spread out after ten minutes, is obtained roxburgh anoectochilus terminal bud seedling, is transplanted into forest land seedbed；
(7) it is 1 by volume with selenium-rich black tea extracting solution and clear water in roxburgh anoectochilus terminal bud growth period:3 dilutions, obtain dilution,
A dilution is uniformly sprayed to blade face and culture medium every other month；Foliage-spray amount is 380ml/m2, the matrix amount of spraying
650ml/m2。
(8) according to existing roxburgh anoectochilus terminal bud conventional cultivation technical standard, relevant maintenance management and pest and disease damage protection is carried out, is ensured
Roxburgh anoectochilus terminal bud normal growth.
Embodiment provided above is the better embodiment of the present invention, is only used for the convenient explanation present invention, not to this hair
The limitation of bright work in any form, any those of ordinary skill in the art, if putting forward skill not departing from the present invention
In the range of art feature, using the equivalent embodiment for locally changing or modifying made by disclosed technology contents, and
Without departing from the technical feature content of the present invention, in the range of still falling within the technology of the present invention feature.
Claims (10)
1. a kind of cultural method of selenium-rich roxburgh anoectochilus terminal bud, it is characterised in that this method comprises the following steps：
(1) extracting solution is prepared：Selenium-rich black tea end or coarse tea are screened, pure clear water is added after crushing, richness is obtained by filtration in extraction
Selenium ruby tea extract；
(2) adventitious bud proliferation：It is explant to choose the interlude stipes without terminal bud and long root, is inoculated on MS culture mediums, two
Breed more than 6 times within a month；
(3) strong seedling culture：It is transplanted to 1/2MS basal mediums and adds and is trained on the strong seedling culture base that selenium-rich black tea extracting solution is formed
Educate to emergence；
(4) selection of land is cultivated：Using the method for hayashishita imitating wild planting roxburgh anoectochilus terminal bud, the forest land small stream more than height above sea level 400m is selected
Limes marginis frescade, nearly broad-leaf forest or pin broad-leaf forest, which is handed over, mixes area, relative air humidity 70%, Chang Feng little or quiet wind, and light transmittance is
25~35%, around there is water source, soil pH is 4.5~6.5；
(5) planting-site is handled：Removal of impurities and smooth, the seedbed fertile soil and selenium-rich that then will be handled well are carried out to the forest land chosen
Soil, sawdust, selenium-rich tea dust make base-material and fully mix, as plant matrix；
(6) roxburgh anoectochilus terminal bud tissue culture rooted seedling is moved into hardening canopy, hardening culture removes after 6 days~14 days, cleans up, use sterilized water
Disinfection is spread out after 8~12 minutes, is obtained roxburgh anoectochilus terminal bud seedling, is transplanted into forest land seedbed；
(7) it is 1 by volume with selenium-rich black tea extracting solution and clear water in roxburgh anoectochilus terminal bud growth period:2~4 dilutions, obtain dilution,
A dilution is uniformly sprayed to blade face and culture medium every other month；
(8) according to existing roxburgh anoectochilus terminal bud conventional cultivation technical standard, relevant maintenance management and pest and disease damage protection is carried out, ensures gold thread
Lotus normal growth.
A kind of 2. cultural method of selenium-rich roxburgh anoectochilus terminal bud according to claim 1, it is characterised in that：Step (1) is specially：
A) selenium-rich black tea end or the coarse tea of Se content >=1.0mg/kg are screened, and is crushed in pulverizer, obtains selenium-rich black tea powder；
B) clear water is added into the selenium-rich tea powder after crushing, control solid-liquid ratio is heated to 80~85 DEG C, constant temperature in 80~90g/L
When stirring 1~2 is small, rotating speed 120r/min, then cooled to room temperature, filtered to obtain filtrate A；
C) clear water is continuously added into the filter residue of step b), control solid-liquid ratio is heated to 70~75 DEG C in 110~120g/L, permanent
When temperature stirring 1~1.5 is small, rotating speed 90r/min, then cooled to room temperature, is obtained by filtration liquor B；
D) boiling water is added into the filter residue of step c), control solid-liquid ratio soaks 20~30min in 140~150g/L, then natural
It is cooled to room temperature, liquor C is obtained by filtration；
E) filtrate A, liquor B and liquor C are uniformly mixed, up to selenium-rich black tea extracting solution.
A kind of 3. cultural method of selenium-rich roxburgh anoectochilus terminal bud according to claim 1, it is characterised in that：Step (2) is specially：No
Bud multiplication：It is explant to choose the interlude stipes without terminal bud and long root, is inoculated on MS culture mediums, while in culture medium
Add the auxiliary material of following concentration：BA3.2~4.5mg/L, NAA0.5~1.0mg/L, 28~30g/L of sucrose and agar 6~
8g/L, breeds more than 6 times in two months.
A kind of 4. cultural method of selenium-rich roxburgh anoectochilus terminal bud according to claim 1 or 3, it is characterised in that：In step (2), training
It is 5.2~5.8 to support base pH value；Condition of culture is：23~27 DEG C, 11~13h/d of light application time of temperature, intensity of illumination 1500~
2000lx。
A kind of 5. cultural method of selenium-rich roxburgh anoectochilus terminal bud according to claim 1, it is characterised in that：It is strong described in step (3)
In seedling culture medium, the also auxiliary material added with following concentration：27~30g/L of sucrose, NAA2.0~3.0mg/L and bananas juice 95~
100g/L。
A kind of 6. cultural method of selenium-rich roxburgh anoectochilus terminal bud according to claim 1 or 5, it is characterised in that：Described in step (3)
The addition concentration of selenium-rich black tea extracting solution is 100~110g/L.
A kind of 7. cultural method of selenium-rich roxburgh anoectochilus terminal bud according to claim 1, it is characterised in that：In step (5), the richness
The Se content of selenium soil is 0.5~1.5mg/kg.
A kind of 8. cultural method of selenium-rich roxburgh anoectochilus terminal bud according to claim 1, it is characterised in that：In step (5), to choosing
Forest land carry out removal of impurities and it is smooth be specially：The weeds for selecting forest land and miscellaneous filling are removed, along contour direction, stepping type, by band
Shape mode is opened up wasteland, and forms seedbed, and with lime disinfection 2~4 days, every square metre was applied 0.05~0.1 kilogram.
A kind of 9. cultural method of selenium-rich roxburgh anoectochilus terminal bud according to claim 1, it is characterised in that：In step (5), seedbed is rotten
The weight ratio for growing soil and selenium-rich soil, sawdust, selenium-rich tea dust is 2~4：2~3：1~2：0.5~1.
A kind of 10. cultural method of selenium-rich roxburgh anoectochilus terminal bud according to claim 1, it is characterised in that：In the step (7),
When uniformly spraying dilution to blade face and culture medium every other month, foliage-spray amount is 300~450ml/m2, matrix sprays
Measure 550~750ml/m2。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711271405.9A CN108012923A (en) | 2017-12-05 | 2017-12-05 | A kind of cultural method of selenium-rich roxburgh anoectochilus terminal bud |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711271405.9A CN108012923A (en) | 2017-12-05 | 2017-12-05 | A kind of cultural method of selenium-rich roxburgh anoectochilus terminal bud |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108012923A true CN108012923A (en) | 2018-05-11 |
Family
ID=62078705
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711271405.9A Pending CN108012923A (en) | 2017-12-05 | 2017-12-05 | A kind of cultural method of selenium-rich roxburgh anoectochilus terminal bud |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108012923A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109937877A (en) * | 2019-03-21 | 2019-06-28 | 漳州职业技术学院 | A kind of breeding method of organic selenium-rich roxburgh anoectochilus terminal bud |
| CN110754362A (en) * | 2019-11-28 | 2020-02-07 | 杭州创美生物科技有限公司 | Culture medium and culture method of selenium-rich anoectochilus roxburghii |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103688854A (en) * | 2013-12-06 | 2014-04-02 | 四川省自然资源科学研究院 | Tissue culture rapid propagation method of Emei anoectochilus formosanus |
| CN106961965A (en) * | 2017-03-27 | 2017-07-21 | 福建华农生物科技有限公司 | A kind of implantation methods of selenium-rich roxburgh anoectochilus terminal bud |
-
2017
- 2017-12-05 CN CN201711271405.9A patent/CN108012923A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103688854A (en) * | 2013-12-06 | 2014-04-02 | 四川省自然资源科学研究院 | Tissue culture rapid propagation method of Emei anoectochilus formosanus |
| CN106961965A (en) * | 2017-03-27 | 2017-07-21 | 福建华农生物科技有限公司 | A kind of implantation methods of selenium-rich roxburgh anoectochilus terminal bud |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109937877A (en) * | 2019-03-21 | 2019-06-28 | 漳州职业技术学院 | A kind of breeding method of organic selenium-rich roxburgh anoectochilus terminal bud |
| CN110754362A (en) * | 2019-11-28 | 2020-02-07 | 杭州创美生物科技有限公司 | Culture medium and culture method of selenium-rich anoectochilus roxburghii |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105557427A (en) | Planting method of selenium-rich passiflora edulis | |
| CN106797861A (en) | A kind of oil tea implantation methods | |
| CN105612986A (en) | Planting method of selenium-rich pitaya | |
| CN106882994A (en) | A kind of implantation methods of the tuber of pinellia | |
| CN105724011B (en) | A kind of plantation breeding method of selenium-rich tree peony | |
| CN107046967A (en) | A kind of implantation methods of selenium-rich beautiful millettia root | |
| CN106358671A (en) | Method for planting gerbera jamesonii | |
| CN105165532A (en) | High-quality tea high-yield planting method | |
| CN104350907A (en) | Technology for planting and disease control of fructus amomi | |
| CN104871756A (en) | Method for planting kiwi fruits | |
| CN107624489A (en) | A kind of implantation methods of selenium-rich lichee | |
| CN107873410A (en) | A kind of cultural method of the selenium-rich stem of noble dendrobium | |
| CN106986691A (en) | A kind of cultural method of spun gold emperor chrysanthemum | |
| CN109121952A (en) | The implantation methods of integrated control Citrus Huanglongbing pathogen | |
| CN105532234B (en) | The breeding method of red heart Hylocereus undatus | |
| CN109328834A (en) | A kind of method of the cultivation of honey locust | |
| CN108651192A (en) | A kind of plantation of green selenium-rich five colors rice and its processing combined method | |
| CN101928190A (en) | Fertilizer special for medicinal Chuzhou chrysanthemum and preparation method thereof | |
| CN108012923A (en) | A kind of cultural method of selenium-rich roxburgh anoectochilus terminal bud | |
| CN108218549A (en) | A kind of drug for being used to prevent pear tree yellowtop | |
| CN106489470A (en) | A kind of Bulbus Fritillariae Cirrhosae method for culturing seedlings | |
| CN102295483A (en) | Preparation method for garden cosmos nutrition fertilizer | |
| CN107295936A (en) | A kind of implantation methods for improving cinnamomum camphora tree cold resistance | |
| CN107409889A (en) | A kind of high-yield planting method of selenium-rich Camellia nitidissima | |
| CN106748362A (en) | A kind of method for planting dendrobium candidum in the tree |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20180511 |