CN108012614A - A kind of method for treating seeds of salt tolerant rice - Google Patents
A kind of method for treating seeds of salt tolerant rice Download PDFInfo
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- CN108012614A CN108012614A CN201711355202.8A CN201711355202A CN108012614A CN 108012614 A CN108012614 A CN 108012614A CN 201711355202 A CN201711355202 A CN 201711355202A CN 108012614 A CN108012614 A CN 108012614A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N35/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical
- A01N35/04—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical containing aldehyde or keto groups, or thio analogues thereof, directly attached to an aromatic ring system, e.g. acetophenone; Derivatives thereof, e.g. acetals
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/06—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings
- A01N43/12—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings condensed with a carbocyclic ring
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/16—Heavy metals; Compounds thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N61/00—Biocides, pest repellants or attractants, or plant growth regulators containing substances of unknown or undetermined composition, e.g. substances characterised only by the mode of action
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Abstract
The invention belongs to Rice Cropping technical field, especially a kind of method for treating seeds of salt tolerant rice, specific method is as follows：（1）Freezing processing will be carried out in rice paddy seed household freezer, takes out, is immersed in heat-insulation soaking in inorganic agent, filtering agent is pulled out, is dried to surface no moisture；The inorganic agent, is made of following raw material：Sodium molybdate, cobalt chloride, potassium fulvate, protocatechualdehyde, Cryptotanshinone, water；（2）Magnetization treatment is carried out, is immersed in ponkan juice and soaks, filtered juice is pulled out；（3）Vacuum freeze drying to moisture content is 20% ~ 22%；Rice Seed Vigor can effectively be strengthened, improve its enzymatic activity, rice paddy seed fast germination is promoted to go out strong sprout, it is effectively improved the physiological property of rice paddy seed, the resistance of rice paddy seed is improved, so as to effectively improve the resistance of rice so that rice has good salt-tolerant trait, the root activity of rice plant can also be effectively kept, plantation can also obtain High-quality high-yield in salt-soda soil.
Description
Technical field
The invention belongs to Rice Cropping technical field, especially a kind of method for treating seeds of salt tolerant rice.
Background technology
One of the salinization of soil causes cultivated area to reduce year by year, and the reason for cause crisis in food, rice is the world
One of cereal crops of upper cultivated area maximum, but current rice does not have salt tolerance generally, so that can not be in salt-soda soil
Planted, instant a small number of its salt tolerance with the rice of salt tolerance are also weaker so that rice suffers from different journeys at present
The salt damage of degree, yield are difficult to improve；So it is current urgent problem to be solved to invent a kind of salt tolerant rice, promote Rice Cropping production
The further development of industry, increases the economic benefit of farmer.
The content of the invention
A kind of in view of the above-mentioned problems, the present invention is intended to provide method for treating seeds of salt tolerant rice.
The present invention is achieved through the following technical solutions：
A kind of method for treating seeds of salt tolerant rice, specific method are as follows：
（1）Freezing processing will be carried out in rice paddy seed household freezer, is taken out, is immersed in the inorganic agent that temperature is 49 ~ 51 DEG C and keeps the temperature
90 ~ 100min is soaked, filtering agent is pulled out, dried to surface no moisture, obtains single treatment rice paddy seed；
The inorganic agent, is made of the raw material of following parts by weight：0.8 ~ 0.9 part of sodium molybdate, 0.25 ~ 0.35 part of cobalt chloride, Huang
Rotten 0.4 ~ 0.5 part of potassium of acid, 0.14 ~ 0.18 part of protocatechualdehyde, 0.21 ~ 0.25 part of Cryptotanshinone, 550 ~ 570 parts of water；
（2）Single treatment rice paddy seed is subjected to magnetization treatment, 36 ~ 40min of immersion, filtered juice in ponkan juice is immersed in and pulls out, obtain
After-treatment rice paddy seed；
（3）By after-treatment rice paddy seed insert temperature be -28 ~ -26 DEG C, absolute pressure be 560 ~ 580Pa under conditions of vacuum
Freeze-drying to moisture content is 20% ~ 22%.
As the further scheme of invention：Step（1）The freezing processing, under conditions of temperature is -17 ~ -16 DEG C
Cryostat handles 26 ~ 28min, and being warming up to -8 ~ -7 DEG C with the heating rate of 0.3 ~ 0.4 DEG C/min continues cryostat processing 17
~ 19min, is warming up to -1.5 ~ -0.5 DEG C with the heating rate of 0.5 ~ 0.6 DEG C/min and continues 34 ~ 36min of cryostat processing.
As the further scheme of invention：The ponkan juice, is prepared according to the following steps：By 35 ~ 37 parts by weight
Belt leather ponkan crushes mashing, inserts constant temperature in the baking box that temperature is 83 ~ 85 DEG C and toasts 14 ~ 16min, takes out, cool down, add
The pectase of 0.23 ~ 0.25 parts by weight is mixed evenly, insert temperature be ferment at constant temperature 34 in 33 ~ 35 DEG C of fermenting case ~
38h, take out, put into 44 ~ 48 parts by weight, mass fraction be 44% ~ 48% ethanol solution in be boiled by slow fire after continue slow boiling endure
26 ~ 30min is boiled, is taken out, cooling, crosses 30 ~ 40 mesh filter clothes, take filtrate, obtain ponkan juice.
As the further scheme of invention：Step（2）The magnetization treatment, in the condition that magnetic field intensity is 380 ~ 390G
6 ~ 7min of lower magnetization treatment, 8 ~ 9min of magnetization treatment under conditions of magnetic field intensity is 1240 ~ 1260G, is 730 in magnetic field intensity
3 ~ 4min of magnetization treatment under conditions of ~ 740G.
Beneficial effects of the present invention：A kind of method for treating seeds of salt tolerant rice provided by the invention, can effectively strengthen
Rice Seed Vigor, improves its enzymatic activity, promotes rice paddy seed fast germination to go out strong sprout, and the physiology for being effectively improved rice paddy seed is special
Property, the resistance of rice paddy seed is improved, so as to effectively improve the resistance of rice so that rice has good salt-tolerant trait, energy
A certain amount of biomass accumulation is kept, is effectively ensured and moisture, mineral element and organic substance is efficiently used, there is potent salt
Alkalineresponse, effectively protects the cell membrane of rice leaf from infringement, efficient defence work is played to the film quality oxidation of cell
With, moreover it is possible to the root activity of rice plant is effectively kept, plantation can also obtain High-quality high-yield in salt-soda soil.
Embodiment
Illustrate the present invention with specific embodiment below, but be not limitation of the present invention.
Embodiment 1
In the embodiment of the present invention, a kind of method for treating seeds of salt tolerant rice, specific method is as follows：
（1）Freezing processing will be carried out in rice paddy seed household freezer, is taken out, is immersed in heat-insulation soaking in the inorganic agent that temperature is 49 DEG C
90 ~ 100min, filtering agent are pulled out, are dried to surface no moisture, obtain single treatment rice paddy seed；
The inorganic agent, is made of the raw material of following parts by weight：0.8 part of sodium molybdate, 0.25 part of cobalt chloride, potassium fulvate 0.4
Part, 0.14 part of protocatechualdehyde, 0.21 part of Cryptotanshinone, 550 parts of water；
（2）Single treatment rice paddy seed is subjected to magnetization treatment, is immersed in ponkan juice and soaks 36min, filtered juice is pulled out, is obtained secondary
Rice seeds treated；
（3）By after-treatment rice paddy seed insert temperature be -28 DEG C, vacuum freeze drying under conditions of absolute pressure is 560Pa
It is 20% ~ 22% to moisture content.
As the further scheme of invention：Step（1）The freezing processing, constant temperature under conditions of being -17 DEG C in temperature
Freezing processing 26min, with the heating rate of 0.3 DEG C/min be warming up to -8 DEG C continue cryostats processing 17min, with 0.5 DEG C/
The heating rate of min is warming up to -1.5 DEG C and continues cryostat processing 34min.
As the further scheme of invention：The ponkan juice, is prepared according to the following steps：By the belt leather of 35 parts by weight
Ponkan crushes mashing, inserts constant temperature in the baking box that temperature is 83 DEG C and toasts 14min, takes out, cooling, adds 0.23 parts by weight
Pectase is mixed evenly, and inserts ferment at constant temperature 34h in the fermenting case that temperature is 33 DEG C, takes out, put into 44 parts by weight, matter
Amount fraction is to continue slow boiling infusion 26min after being boiled by slow fire in 44% ethanol solution, is taken out, cooling, crosses 30 mesh filter clothes, take filter
Liquid, obtains ponkan juice.
As the further scheme of invention：Step（2）The magnetization treatment, the magnetic under conditions of magnetic field intensity is 380G
Change processing 6min, the magnetization treatment 8min under conditions of magnetic field intensity is 1240G, magnetizes under conditions of magnetic field intensity is 730G
Handle 3min.
Embodiment 2
In the embodiment of the present invention, a kind of method for treating seeds of salt tolerant rice, specific method is as follows：
（1）Freezing processing will be carried out in rice paddy seed household freezer, is taken out, is immersed in heat-insulation soaking in the inorganic agent that temperature is 50 DEG C
95min, filtering agent are pulled out, are dried to surface no moisture, obtain single treatment rice paddy seed；
The inorganic agent, is made of the raw material of following parts by weight：0.85 part of sodium molybdate, 0.3 part of cobalt chloride, potassium fulvate 0.45
Part, 0.16 part of protocatechualdehyde, 0.23 part of Cryptotanshinone, 560 parts of water；
（2）Single treatment rice paddy seed is subjected to magnetization treatment, is immersed in ponkan juice and soaks 38min, filtered juice is pulled out, is obtained secondary
Rice seeds treated；
（3）By after-treatment rice paddy seed insert temperature be -27 DEG C, vacuum freeze drying under conditions of absolute pressure is 570Pa
It is 20% ~ 22% to moisture content.
As the further scheme of invention：Step（1）The freezing processing, it is permanent under conditions of being -16.5 DEG C in temperature
Warm freezing processing 27min, is warming up to -7.5 DEG C with the heating rate of 0.35 DEG C/min and continues cryostat processing 18min, with
The heating rate of 0.55 DEG C/min is warming up to -1 DEG C and continues cryostat processing 35min.
As the further scheme of invention：The ponkan juice, is prepared according to the following steps：By the belt leather of 36 parts by weight
Ponkan crushes mashing, inserts constant temperature in the baking box that temperature is 84 DEG C and toasts 15min, takes out, cooling, adds 0.24 parts by weight
Pectase is mixed evenly, and inserts ferment at constant temperature 36h in the fermenting case that temperature is 34 DEG C, takes out, put into 46 parts by weight, matter
Amount fraction is to continue slow boiling infusion 28min after being boiled by slow fire in 46% ethanol solution, is taken out, cooling, crosses 35 mesh filter clothes, take filter
Liquid, obtains ponkan juice.
As the further scheme of invention：Step（2）The magnetization treatment, the magnetic under conditions of magnetic field intensity is 385G
Change processing 6.5min, the magnetization treatment 8.5min under conditions of magnetic field intensity is 1250G, under conditions of magnetic field intensity is 735G
Magnetization treatment 3.5min.
Embodiment 3
In the embodiment of the present invention, a kind of method for treating seeds of salt tolerant rice, specific method is as follows：
（1）Freezing processing will be carried out in rice paddy seed household freezer, is taken out, is immersed in heat-insulation soaking in the inorganic agent that temperature is 51 DEG C
100min, filtering agent are pulled out, are dried to surface no moisture, obtain single treatment rice paddy seed；
The inorganic agent, is made of the raw material of following parts by weight：0.9 part of sodium molybdate, 00.35 part of cobalt chloride, potassium fulvate 0.5
Part, 0.18 part of protocatechualdehyde, 0.25 part of Cryptotanshinone, 570 parts of water；
（2）Single treatment rice paddy seed is subjected to magnetization treatment, is immersed in ponkan juice and soaks 40min, filtered juice is pulled out, is obtained secondary
Rice seeds treated；
（3）By after-treatment rice paddy seed insert temperature be -26 DEG C, vacuum freeze drying under conditions of absolute pressure is 580Pa
It is 20% ~ 22% to moisture content.
As the further scheme of invention：Step（1）The freezing processing, constant temperature under conditions of being -16 DEG C in temperature
Freezing processing 28min, with the heating rate of 0.4 DEG C/min be warming up to -7 DEG C continue cryostats processing 19min, with 0.6 DEG C/
The heating rate of min is warming up to -0.5 DEG C and continues cryostat processing 36min.
As the further scheme of invention：The ponkan juice, is prepared according to the following steps：By the belt leather of 37 parts by weight
Ponkan crushes mashing, inserts constant temperature in the baking box that temperature is 85 DEG C and toasts 16min, takes out, cooling, adds 0.25 parts by weight
Pectase is mixed evenly, and inserts ferment at constant temperature 38h in the fermenting case that temperature is 35 DEG C, takes out, put into 44 ~ 48 weight
Part, mass fraction be 48% ethanol solution in be boiled by slow fire after continue slow boiling infusion 30min, take out, cooling, cross 40 mesh filter clothes,
Filtrate is taken, obtains ponkan juice.
As the further scheme of invention：Step（2）The magnetization treatment, the magnetic under conditions of magnetic field intensity is 390G
Change processing 7min, the magnetization treatment 9min under conditions of magnetic field intensity is 1260G, under conditions of magnetic field intensity is 730 ~ 740G
Magnetization treatment 4min.
Comparative example 1
The rice paddy seed normal treatment process of the prior art
Comparative example 2
A kind of method for treating seeds of salt tolerant rice, difference lies in step with embodiment 1（1）Without freezing processing, other
Condition all same.
Comparative example 3
A kind of method for treating seeds of salt tolerant rice, difference lies in by step with embodiment 1（1）The inorganic agent water
Instead of other conditions all same.
Comparative example 4
A kind of method for treating seeds of salt tolerant rice, difference lies in step with embodiment 1（2）Without magnetization treatment, other
Condition all same.
Comparative example 5
A kind of method for treating seeds of salt tolerant rice, difference lies in by step with embodiment 1（2）The ponkan juice water
Instead of other conditions all same.
Comparative example 6
A kind of method for treating seeds of salt tolerant rice, difference lies in by step with embodiment 1（3）The vacuum refrigeration is done
Dry processing is replaced with drying processing, other conditions all same.
At with a collection of " white No. 9 long " rice paddy seed using the processing method of embodiment and the processing method of comparative example
Reason, is sowed after processing, is sowed 9 mu, is equally divided into 9 groups, per 1 mu of multicast kind, in addition to method for treating seeds is different, other
Part all same, booting carry out salt stress processing initial stage, Stress treatment are carried out with the NaCl of 100mmol/L, pregnant after handling 15 days
Fringe mid-term proceeds by the measure of defence enzyme activity, malonaldehyde and membrane permeability, as a result such as table 1：
The comparing result of 1 embodiment of table and comparative example
| SOD activity（U/g） | CAT activity（U/g） | Mda content（μmol/g） | Membrane permeability (%) | |
| Embodiment 1 | 995.21 | 949.77 | 0.011 | 8.59 |
| Embodiment 2 | 996.45 | 952.65 | 0.009 | 8.51 |
| Embodiment 3 | 998.65 | 954.66 | 0.011 | 8.54 |
| Comparative example 1 | 386.53 | 321.43 | 0.044 | 77.89 |
| Comparative example 2 | 789.44 | 746.78 | 0.024 | 30.65 |
| Comparative example 3 | 732.43 | 676.89 | 0.032 | 40.76 |
| Comparative example 4 | 756.78 | 708.76 | 0.026 | 32.43 |
| Comparative example 5 | 769.87 | 724.79 | 0.027 | 33.56 |
| Comparative example 6 | 751.48 | 718.69 | 0.026 | 31.98 |
As it can be seen from table 1 the method for the present invention can be obviously improved the defence enzyme activity of rice, mda content and film are reduced
Permeability, has good salt-tolerant trait.
Claims (4)
1. a kind of method for treating seeds of salt tolerant rice, it is characterised in that specific method is as follows：
（1）Freezing processing will be carried out in rice paddy seed household freezer, is taken out, is immersed in the inorganic agent that temperature is 49 ~ 51 DEG C and keeps the temperature
90 ~ 100min is soaked, filtering agent is pulled out, dried to surface no moisture, obtains single treatment rice paddy seed；
The inorganic agent, is made of the raw material of following parts by weight：0.8 ~ 0.9 part of sodium molybdate, 0.25 ~ 0.35 part of cobalt chloride, Huang
Rotten 0.4 ~ 0.5 part of potassium of acid, 0.14 ~ 0.18 part of protocatechualdehyde, 0.21 ~ 0.25 part of Cryptotanshinone, 550 ~ 570 parts of water；
（2）Single treatment rice paddy seed is subjected to magnetization treatment, 36 ~ 40min of immersion, filtered juice in ponkan juice is immersed in and pulls out, obtain
After-treatment rice paddy seed；
（3）By after-treatment rice paddy seed insert temperature be -28 ~ -26 DEG C, absolute pressure be 560 ~ 580Pa under conditions of vacuum
Freeze-drying to moisture content is 20% ~ 22%.
2. the method for treating seeds of salt tolerant rice according to claim 1, it is characterised in that step（1）The freezing
Processing, cryostat handles 26 ~ 28min under conditions of being -17 ~ -16 DEG C in temperature, with the heating rate of 0.3 ~ 0.4 DEG C/min
It is warming up to -8 ~ -7 DEG C and continues cryostats and handles 17 ~ 19min, -1.5 are warming up to the heating rate of 0.5 ~ 0.6 DEG C/min ~ -
0.5 DEG C is continued cryostat and handles 34 ~ 36min.
3. the method for treating seeds of salt tolerant rice according to claim 1, it is characterised in that the ponkan juice, by with
It is prepared by lower step：The belt leather ponkan of 35 ~ 37 parts by weight is crushed into mashing, is inserted permanent in the baking box that temperature is 83 ~ 85 DEG C
Temperature 14 ~ 16min of baking, takes out, and cooling, the pectase for adding 0.23 ~ 0.25 parts by weight is mixed evenly, and inserts temperature as 33
34 ~ 38h of ferment at constant temperature in ~ 35 DEG C of fermenting case, takes out, and puts into 44 ~ 48 parts by weight, the ethanol that mass fraction is 44% ~ 48%
Continue slow boiling 26 ~ 30min of infusion after being boiled by slow fire in solution, take out, cooling, crosses 30 ~ 40 mesh filter clothes, take filtrate, obtain ponkan juice.
4. the method for treating seeds of salt tolerant rice according to claim 1, it is characterised in that step（2）The magnetization
Processing, 6 ~ 7min of magnetization treatment under conditions of magnetic field intensity is 380 ~ 390G, in the condition that magnetic field intensity is 1240 ~ 1260G
8 ~ 9min of lower magnetization treatment, 3 ~ 4min of magnetization treatment under conditions of magnetic field intensity is 730 ~ 740G.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110896699A (en) * | 2019-12-06 | 2020-03-24 | 湖南袁禾农业科技有限公司 | Method for treating saline-alkali soil by using cord grass and mangrove forest |
| RU2746137C1 (en) * | 2020-08-19 | 2021-04-07 | Федеральное государственное бюджетное образовательное учреждение высшего образования "Кубанский государственный аграрный университет имени И.Т. Трубилина" | Method of pre-sowing treatment of rice seeds with cobalt in the conditions of the krasnodar region |
| CN113207908A (en) * | 2021-06-04 | 2021-08-06 | 广东海洋大学 | Preparation and application method of saline-alkali-resistant rice seed initiator |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110896699A (en) * | 2019-12-06 | 2020-03-24 | 湖南袁禾农业科技有限公司 | Method for treating saline-alkali soil by using cord grass and mangrove forest |
| RU2746137C1 (en) * | 2020-08-19 | 2021-04-07 | Федеральное государственное бюджетное образовательное учреждение высшего образования "Кубанский государственный аграрный университет имени И.Т. Трубилина" | Method of pre-sowing treatment of rice seeds with cobalt in the conditions of the krasnodar region |
| CN113207908A (en) * | 2021-06-04 | 2021-08-06 | 广东海洋大学 | Preparation and application method of saline-alkali-resistant rice seed initiator |
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