CN107970203A - A kind of biofermentation surfactant and preparation method thereof - Google Patents
A kind of biofermentation surfactant and preparation method thereof Download PDFInfo
- Publication number
- CN107970203A CN107970203A CN201810070935.5A CN201810070935A CN107970203A CN 107970203 A CN107970203 A CN 107970203A CN 201810070935 A CN201810070935 A CN 201810070935A CN 107970203 A CN107970203 A CN 107970203A
- Authority
- CN
- China
- Prior art keywords
- component
- parts
- biofermentation
- raw material
- surfactant
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/99—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from microorganisms other than algae or fungi, e.g. protozoa or bacteria
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
- A61K8/34—Alcohols
- A61K8/345—Alcohols containing more than one hydroxy group
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
- A61K8/735—Mucopolysaccharides, e.g. hyaluronic acid; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/84—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds obtained by reactions otherwise than those involving only carbon-carbon unsaturated bonds
- A61K8/88—Polyamides
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/004—Aftersun preparations
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/59—Mixtures
- A61K2800/592—Mixtures of compounds complementing their respective functions
- A61K2800/5922—At least two compounds being classified in the same subclass of A61K8/18
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Veterinary Medicine (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Epidemiology (AREA)
- Birds (AREA)
- Dermatology (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Gerontology & Geriatric Medicine (AREA)
- Tropical Medicine & Parasitology (AREA)
- Botany (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Emergency Medicine (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a kind of biofermentation surfactant and preparation method thereof, including component A, B component and component C；The component A includes the raw material of following parts by weight：Bacillus/50~70 parts of glutamic acid fermentation Product filtrate, 15~25 parts of water, 0.05~0.2 part of Sodium Hyaluronate, 1~3 part of glycerine, 1~4 part of glycerine polymethacrylates, 1~3.5 part of diglycerol, 0.04~0.08 part of EDETATE SODIUM；The B component includes the raw material of following parts by weight：2~5 parts of oat beta glucan, 1~4.5 part of edelweiss extract, purple 0.5~3.5 part of ball algae extract, 1 0.0005~0.002 part of oligopeptides, 1~4 part of citrus extracts；The component C includes the raw material of following parts by weight：1~4 part of glycerine, 0.05~2 part of 1,2 pentanediol, 0.1~0.5 part of Sensiva SC50.The present invention biofermentation surfactant there is long-acting moisturizing water lock, block the melanin of skin generate, balance skin acid-base property, anti-aging, releive, whitening, nourishing protect and other effects.
Description
Technical field
The present invention relates to technical field of skin care, in particular to a kind of biofermentation surfactant and preparation method thereof.
Background technology
As environment is serious all the more with ecological disruption, at the same habits and customs by people and age etc. it is comprehensive influence, skin
Many problems, such as skin aging occur in skin, there is wrinkle, pigmentation, skin dimness etc..Skin problem for more than, people
Developed some with replenishing water and preserving moisture, anti-wrinkle, Bearberry Extract skin care item, the surfactant in skin care item, in people
The amount that its in routine use uses is bigger.
Surfactant is also referred to as toner, firming lotion, toner etc., and in the market species is various, and also level is uneven for respective function,
Quality differs greatly.Most of existing surfactant may add the chemical industry such as some petroleum by-products for profit-push
Raw material, it is common to use chemical preservative, long-time service may produce injury to human body.As people are to natural safety and environmental protection
The demand of skin care item is higher and higher, these surfactant cannot meet the requirement of people.
The content of the invention
In view of this, a kind of biofermentation surfactant provided by the invention and preparation method thereof, preferably overcomes above-mentioned
Problem and defect existing in the prior art, using bacillus/glutamic acid fermentation Product filtrate as main component, have long-acting guarantor
Wet water lock, blocks the melanin of skin to generate, balance skin acid-base property and other effects, while compounds Sodium Hyaluronate, oat β~Portugal
Glycan, edelweiss extract, purple ball algae extract, oligopeptides, citrus extracts, glycerine and Sensiva SC50 etc., collaboration
Excellent anti-aging is played, releives, is calm, whitening and nourishing protection and other effects, fine wrinkles can be smoothed, improve skin bullet
Property, improve dermatoglyph degree, impart good transdermal absorbent properties；And fibroblast can be promoted to synthesize collagen egg
In vain, promote wound healing, repair impaired skin, the skin that can be directed to after shining is repaired, and it is aobvious to prevent that pigmentation etc. from playing
Write effect.
A kind of biofermentation surfactant, including component A, B component and component C；
The component A includes the raw material of following parts by weight：Bacillus/50~70 parts of glutamic acid fermentation Product filtrate,
15~25 parts of water, 0.05~0.2 part of Sodium Hyaluronate, 1~3 part of glycerine, 1~4 part of glycerine polymethacrylates, diglycerol 1
~3.5 parts, 0.04~0.08 part of EDETATE SODIUM；
The B component includes the raw material of following parts by weight：2~5 parts of avenabeta glucosan, edelweiss extract 1
~4.5 parts, purple 0.5~3.5 part of ball algae extract, 0.0005~0.002 part of oligopeptides -1,1~4 part of citrus extracts；
The component C includes the raw material of following parts by weight：1~4 part of glycerine, 0.05~2 part of 1,2- pentanediols, ethyl hexyl
0.1~0.5 part of base glycerol.
The bacillus/glutamic acid fermentation Product filtrate is prepared by the following method to obtain：
(1) Bacillus strain is accessed in gnotobasis in sterilized LB culture mediums, carries out shaking table culture, obtain
Seed liquor；
(2) seed liquor is accessed in fermentation tank by 1~10% inoculum concentration and fermented, obtain fermentation of bacillus
Liquid；
(3) zymotic fluid is taken into supernatant after centrifugation, is then concentrated by ultrafiltration, obtain bacillus/glutamic acid
Tunning filtrate.
Further, the component C further includes the raw material of following parts by weight：3~6 parts of floral type plant extracts.
Further, during the shaking table culture：Shaking table temperature is 30~40 DEG C, and shaking speed is 150~200rpm,
Incubation time is 15~18h.
Further, be equipped with the fermentation tank 50~400L it is sterilized after fluid nutrient medium, the Liquid Culture
Base includes following components in percentage by weight：Dusty yeast 0.5~3%, ammonium chloride 1~2.5%, sodium glutamate 5~10%, phosphoric acid
Disodium hydrogen 0.1~0.3%, potassium dihydrogen phosphate 0.02~0.15%, sodium chloride 1~2%, the water of glucose 2~5% and surplus.
Further, the sterilization process includes：Go out under conditions of pressure is 0.01MPa and temperature is 115~122 DEG C
20~40min of bacterium.
Further, the fermentation process includes：Temperature be 30~40 DEG C, throughput for 50~400vvm and 100~
Ferment 24~64h under the stir speed (S.S.) of 700rpm.
Further, in step (3), further included after the ultrafiltration concentration：It is in pressure by obtained ultrafiltration concentration liquid
20~40min of sterilizing under conditions of 0.01MPa and temperature are 115~122 DEG C.
Further, the floral type plant extracts is rose extract, Herba Lysimachiae foenumgraeci extract or orchid extract.
Present invention also offers a kind of preparation method of biofermentation surfactant, the biofermentation surfactant is above-mentioned
Biofermentation surfactant, the preparation method include：
(1) first the Sodium Hyaluronate in component A is scattered in glycerine, each raw material in component A is then added into main cooker
In be uniformly mixed；When main cooker is warming up to 80~84 DEG C, 3~5min of homogeneous, then keep the temperature 20~25min sterilizings；
(2) it is stirred each raw material that main cooker is added in B component when being cooled to 45 DEG C；
(3) each raw material for continuing to make main cooker add in component C when being cooled to 43 DEG C is stirred, then 3~5min of homogeneous,
Continue 30~40min of stirring.
Further, the stir speed (S.S.) is 100~700rpm.
Compared with prior art, a kind of beneficial effect of biofermentation surfactant of the invention and preparation method thereof is：
The biofermentation surfactant of the present invention is rich in using bacillus/glutamic acid fermentation Product filtrate as main component
Polyglutamic acid, has long-acting moisturizing water lock, blocks the melanin of skin to generate, balance skin acid-base property and other effects, compounds at the same time
Sodium Hyaluronate, oat β~glucan, edelweiss extract, purple ball algae extract, oligopeptides, citrus extracts, glycerine and
Sensiva SC50 etc., collaboration play excellent anti-aging, releive, calm, whitening and nourish protection and other effects, can smooth thin
Small wrinkle, improves skin elasticity, improves dermatoglyph degree, imparts good transdermal absorbent properties；And it can promote into fibre
Smooth muscle cells collagen synthesis albumen is tieed up, promotes wound healing, repairs impaired skin, the skin that can be directed to after shining is repaired, and is prevented
Pigmentation etc. plays remarkable result.In addition, the biofermentation surfactant of the present invention is safe and non-toxic, non-stimulated.
To enable the above objects, features and advantages of the present invention to become apparent, preferred embodiment cited below particularly, makees detailed
It is described as follows.
Embodiment
For the ease of understanding the present invention, elaborate with reference to the mode of embodiment to technical scheme,
Many details are elaborated in the following description in order to fully understand the present invention.
But the invention can be embodied in many other ways as described herein, those skilled in the art can be with
Similar improvement is done in the case of without prejudice to intension of the present invention, therefore the present invention is not limited to the specific embodiments disclosed below.
Unless otherwise defined, all technologies used herein and scientific terminology have and the common skill of fields of the present invention
The normally understood identical implication of art personnel.When there is a conflict, the definition in this specification shall prevail.
Term as used herein：
Term "comprising" used herein, " comprising ", " having ", " containing " or its any other deformation, it is intended that covering
Non-exclusionism includes.Wanted for example, composition, step, method, product or device comprising listed elements are not necessarily limited to those
Element, but can include not expressly listed other key elements or such a composition, step, method, product or device it is intrinsic
Key element.
Conjunction " by ... form " exclude any key element do not pointed out, step or component.If be used in claim,
This phrase will make claim be closed, it is not included the material in addition to the material of those descriptions, but relative
Except customary impurities.When phrase " by ... form " be rather than immediately following theme in the clause that appears in claim main body after
When, it is only limited to the key element described in the clause；Other key elements be not excluded the claim as a whole it
Outside.
Equivalent, concentration or other values or parameter are excellent with scope, preferred scope or a series of upper limit preferred values and lower limit
During the Range Representation that choosing value limits, this, which is appreciated that, specifically discloses by any range limit or preferred value and any scope
All scopes that any pairing of lower limit or preferred value is formed, regardless of whether the scope separately discloses.For example, when open
Scope " when 1~5 ", described scope should be interpreted as including scope " 1~4 ", " 1~3 ", " 1~2 ", " 1~2 and 4~
5 ", " 1~3 and 5 " etc..When number range is described herein, unless otherwise stated, otherwise the scope is intended to include its end
Value and all integers and fraction within the range.
"and/or" is used to represent that one of illustrated situation or both may to occur, for example, A and/or B includes (A
And B) and (A or B).
A kind of biofermentation surfactant, including component A, B component and component C；
The component A includes the raw material of following parts by weight：
Bacillus/such as 50 parts of 50~70 parts of glutamic acid fermentation Product filtrate, 55 parts, 60 parts, 65 parts or 70 parts；
Such as 15 parts, 18 parts, 20 parts, 22 parts or 25 parts of 15~25 parts of water；
Such as 0.05 part, 0.08 part, 0.1 part, 0.15 part or 0.2 part of 0.05~0.2 part of Sodium Hyaluronate；
Such as 1 part, 1.5 parts, 2 parts, 2.5 parts or 3 parts of 1~3 part of glycerine；
Such as 1 part, 2 parts, 3 parts or 4 parts of 1~4 part of glycerine polymethacrylates；
Such as 1 part, 1.5 parts, 2 parts, 2.5 parts, 3 parts or 3.5 parts of 1~3.5 part of diglycerol；
Such as 0.04 part, 0.05 part, 0.06 part, 0.07 part or 0.08 part of 0.04~0.08 part of EDETATE SODIUM.
The B component includes the raw material of following parts by weight：
Such as 2 parts, 3 parts, 4 parts or 5 parts of 2~5 parts of avenabeta glucosan；
Such as 1 part, 2 parts, 3 parts, 4 parts or 4.5 parts of 1~4.5 part of edelweiss extract；
Purple such as 0.5 part, 1 part, 1.5 parts, 2 parts, 2.5 parts, 3 parts or 3.5 parts of 0.5~3.5 part of ball algae extract；
0.0005~0.002 part of oligopeptides -1 as 0.0005 part, 0.0008 part, 0.001 part, 0.0012 part, 0.0015 part,
0.0018 part or 0.002 part etc.；
Such as 1 part, 2 parts, 3 parts or 4 parts of 1~4 part of citrus extracts；
The component C includes the raw material of following parts by weight：
Such as 1 part, 2 parts, 3 parts or 4 parts of 1~4 part of glycerine；
Such as 0.05 part, 0.1 part, 0.5 part, 1 part, 1.2 parts, 1.5 parts or 2 parts of 0.05~2 part of 1,2- pentanediols；
Such as 0.1 part, 0.2 part, 0.3 part, 0.4 part or 0.5 part of 0.1~0.5 part of Sensiva SC50.
The bacillus/glutamic acid fermentation Product filtrate is prepared by the following method to obtain：
(1) Bacillus strain is accessed in gnotobasis in sterilized LB culture mediums, carries out shaking table culture, obtain
Seed liquor.It should be noted that the formula of LB culture mediums is as follows：Tryptone 10g/L, yeast extract 5g/L, sodium chloride
Need to add tryptone 10g, yeast extract 5g and sodium chloride 10g in 10g/L, i.e. 0.95L deionized waters.
(2) seed liquor is accessed in fermentation tank by 1~10% such as 1%, 3%, 5%, 8% or 10% inoculum concentration
Ferment, obtain fermentation of bacillus liquid.
(3) zymotic fluid is taken into supernatant after centrifugation, is then concentrated by ultrafiltration, obtain bacillus/glutamic acid
Tunning filtrate.
It should be noted that the preferred bafillus natto of above-mentioned bacillus or bacillus subtilis etc., its is fermented
To product in main component be polyglutamic acid, molecular weight has long-acting moisturizing water lock between 10~1,000,000, blocks skin
Melanin generation, balance skin acid-base property and other effects.
Sodium Hyaluronate can improve skin-nourishing metabolism as a kind of good moisturizing ingredient, make that skin is tender, light
Slide, go to wrinkle, increase elasticity, prevent aging, be good skin penetration enhancer again while moisturizing.
Glycerine, glycerine polymethacrylates and diglycerol collaboration are used as moisturizer, make skin care item moistening effect more preferable.On
EDETATE SODIUM is stated as a kind of mineral sequestration agent.
Avenabeta glucosan is a kind of water-soluble dietary fiber, because its stickiness having hinders the materials such as starch, protein
Digestion and absorption, so can have some extremely advantageous physiological functions to human body：With significant reducing blood lipid, it is hypoglycemic and
Improve immunocompetence etc..In addition, it can also accelerate to determine the immunocyte of crowd, the reaction to bacterium infection simultaneously controls bacterium
The position of infection, makes infection face recover as early as possible, as the active ingredient of cosmetics, can improve resisting skin allergy ability, activation
Immune function, delay skin aging.
Edelweiss is the famous Alpine Wild Ornamental Plants in Europe, shielded plant is classified as by many countries, more by Switzerland's honor
For national flower.Certainly imperial ability of edelweiss tool height, can resist atrocious weather and other growing environments, its essential ingredient contains rich
Rich mineral matter, releives skin tool, calm, whitening and nourishes protective effect, be natural beauty sage product.Edelweiss extracts
The elite composition of edelweiss is applied, and can effectively be releived, calm skin, be discharged harmful substance in skin, have preferably
Whitening, moisturizing, moisturizing and anti-wrinkle effect；It can give skin thoughtful nourishing care, allow skin to keep lustrous and transparent white tender and lovely for a long time；Its day
Right whitening composition and aromatising essence, can gentle deep layer cleaning skin, remove pollution in the grease and pore of epidermis, it is easypro at the same time
Slow skin tense situation, it is soft not tight；And its natural whiting component, can penetrate into skin, catabolism impurity is helped, is prevented
Only pigmentation, pale gloss soft and smooth careful with rear skin.
Contain oligosaccharide and essential trace element in purple ball algae extract, can help to shrink vein and the micro- blood of skin
Pipe, improves the symptoms such as red blood silk, inflammation, livid ring around eye, oedema, while promotes Skin Cell to regenerate, and improves skin appearance.
Oligopeptides -1 also known as epidermal growth factor (EGF), the relative molecular weight being made of 53 amino acid are about 6200
Micromolecule polypeptide, its performance is gentle, can strongly promote various epidermal tissues to grow, and plays the role of repairing skin.
Contain substantial amounts of flavone component in citrus extracts, there is inoxidizability, the oxygen radical in skin can be eliminated.
Sensiva SC50 in component C can effectively play bacteriostasis.
Seen from the above description, biofermentation surfactant of the invention is made with bacillus/glutamic acid fermentation Product filtrate
For main component, rich in polyglutamic acid, there is long-acting moisturizing water lock, block the melanin of skin to generate, balance skin acid-base property
And other effects, while compound Sodium Hyaluronate, oat β~glucan, edelweiss extract, purple ball algae extract, oligopeptides, mandarin orange
Tangerine extract, glycerine and Sensiva SC50 etc., collaboration play excellent anti-aging, releive, calm, whitening and nourish protection etc.
Effect, can smooth fine wrinkles, improve skin elasticity, improve dermatoglyph degree, impart good transdermal absorbent properties；
And fibroblast can be promoted to synthesize collagen, promote wound healing, repair impaired skin, the skin after shining can be directed to
Repaired, prevent pigmentation etc. from playing remarkable result.In addition, the biofermentation surfactant of the present invention is safe and non-toxic, stingless
Swash.
Further, the component C further includes the raw material of following parts by weight：3~6 parts of floral type plant extracts such as 3
Part, 4 parts, 5 parts or 6 parts etc..
Preferably, the floral type plant extracts is rose extract, Herba Lysimachiae foenumgraeci extract or orchid extract.It is above-mentioned
In addition to rose extract, Herba Lysimachiae foenumgraeci extract and orchid extract increase the fragrance of surfactant except may be used as plant essence,
Whitening can also be played, soothed the skin and anti-aging and other effects.Certain present invention is not limited in above-mentioned floral type plant extract
Thing, such as can also be jasmine flower extract, camellia flower extract etc..
Above-mentioned purple ball algae extract, citrus extracts, Flos Rosae Rugosae extract, Herba Lysimachiae foenumgraeci extract and orchid extract are
Commercial product.
Preferably, above-mentioned water is deionized water.Can certainly be distilled water or spring living.
Preferably, during the shaking table culture：Shaking table temperature is 30~40 DEG C；Shaking speed is 150~200rpm；Training
It is 15~18h to support the time.
Preferably, be equipped with the fermentation tank 50~400L it is sterilized after fluid nutrient medium.
The fluid nutrient medium includes following components in percentage by weight：Dusty yeast 0.5~3%, ammonium chloride 1~2.5%,
Sodium glutamate 5~10%, disodium hydrogen phosphate 0.1~0.3%, potassium dihydrogen phosphate 0.02~0.15%, sodium chloride 1~2%, grape
The water of sugar 2~5% and surplus.
Preferably, the sterilization process includes：Sterilize under conditions of pressure is 0.01MPa and temperature is 115~122 DEG C
20~40min.
Preferably, the fermentation process includes：Temperature be 30~40 DEG C, throughput for 50~400vvm and 100~
Ferment 24~64h under the stir speed (S.S.) of 700rpm.
Preferably, in step (3), further included after the ultrafiltration concentration：It is in pressure by obtained ultrafiltration concentration liquid
20~40min of sterilizing under conditions of 0.01MPa and temperature are 115~122 DEG C.
Present invention also offers a kind of preparation method of biofermentation surfactant, the biofermentation surfactant is above-mentioned
Biofermentation surfactant, the preparation method include：
(1) first the Sodium Hyaluronate in component A is scattered in glycerine, each raw material in component A is then added into main cooker
In be uniformly mixed；When main cooker is warming up to 80~84 DEG C, 3~5min of homogeneous, then keep the temperature 20~25min sterilizings；
(2) it is stirred each raw material that main cooker is added in B component when being cooled to 45 DEG C；
(3) each raw material for continuing to make main cooker add in component C when being cooled to 43 DEG C is stirred, then 3~5min of homogeneous,
Continue 30~40min of stirring.
Preferably, the stir speed (S.S.) is 100~700rpm.
It should be noted that first the Sodium Hyaluronate in component A can be scattered in using other containers in step (1) sweet
In oil, then sequentially add in main cooker and be uniformly mixed with other raw materials in component A again；Certainly can also be first in main cooker
Glycerine is added, after the Sodium Hyaluronate in component A is scattered in glycerine, sequentially adds other raw materials in component A.
For the ease of understanding the present invention, technical scheme is further illustrated with reference to embodiment.Applicant
Statement, the present invention illustrate the detailed process equipment of the present invention and technological process, but not office of the invention by following embodiments
It is limited to following detailed process equipments and technological process, that is, does not mean that the present invention should rely on following detailed process equipment and technique streams
Cheng Caineng is implemented.Person of ordinary skill in the field is it will be clearly understood that any improvement in the present invention, to each original of product of the present invention
The equivalence replacement of material and the addition of auxiliary element, the selection of concrete mode etc., all fall within protection scope of the present invention and open model
Within enclosing.
Embodiment 1
A kind of biofermentation surfactant, including component A, B component and component C；
The component A includes the raw material of following parts by weight：Bacillus/50 parts of glutamic acid fermentation Product filtrate, go from
Sub- 15 parts of water, 0.05 part of Sodium Hyaluronate, 3 parts of glycerine, 4 parts of glycerine polymethacrylates, 1 part of diglycerol, EDETATE SODIUM
0.04 part；
The B component includes the raw material of following parts by weight：2 parts of avenabeta glucosan, edelweiss extract 4.5
Part, purple 0.5 part of ball algae extract, 0.0005 part of oligopeptides -1,4 parts of citrus extracts；
The component C includes the raw material of following parts by weight：3 parts of rose extract, 1 part of glycerine, 1,2- pentanediols 0.05
Part, 0.1 part of Sensiva SC50.
Above-mentioned bacillus/glutamic acid fermentation Product filtrate is prepared by the following method to obtain：
(1) LB culture mediums are sterilized 40min under conditions of pressure is 0.01MPa and temperature is 115 DEG C, then by gemma
Bacillus strain is accessed in gnotobasis in the LB culture mediums after sterilizing, is subsequently placed in that temperature is 30 DEG C and rotating speed is 150rpm's
Shaking table culture 18h is carried out in shaking table, obtains seed liquor.
(2) seed liquor access is equipped with the fermentation tank of 50L fluid nutrient mediums by 1% inoculum concentration, is 30 in temperature
DEG C, throughput is the 64h that ferments under the stir speed (S.S.) of 50vvm and 100rpm, obtains fermentation of bacillus liquid, the fluid nutrient medium
Including following components in percentage by weight：Dusty yeast 0.5%, ammonium chloride 1%, sodium glutamate 10%, disodium hydrogen phosphate 0.1%,
Potassium dihydrogen phosphate 0.02%, sodium chloride 2%, the water of glucose 2% and surplus.
(3) use continuous stream tube centrifuge by zymotic fluid through collected after centrifugation supernatant, then using hollow-fibre membrane
Supernatant is concentrated by ultrafiltration in filtration system, then concentrate after ultrafiltration is carried out autoclave sterilization, and sterilising conditions are set as：
115 DEG C of temperature, pressure 0.01MPa and time are 40min, obtain bacillus/glutamic acid fermentation Product filtrate.
The preparation method of above-mentioned biofermentation surfactant is as follows：
(1) main cooker is cleaned up and sterilized before producing, checks whether raw material is correct before feeding intake.
(2) first the Sodium Hyaluronate in component A is scattered in glycerine, each raw material in component A is then added into main cooker
Middle mixing, opens stirring, stir speed (S.S.) 500rpm；Then main cooker is warming up to 80 DEG C, homogeneous 3min, then keeps the temperature 20min and go out
Bacterium, whole process keep the stir speed (S.S.) of 500rpm；
(3) it is 300rpm to adjust stir speed (S.S.), main cooker is cooled to each raw material stirring added at 45 DEG C in B component
Mixing；
(4) each raw material for continuing to make main cooker add in component C when being cooled to 43 DEG C is stirred, then homogeneous 3min, after
Stop stirring after continuous stirring 30min, examine, discharging, obtain biofermentation surfactant.
Embodiment 2
A kind of biofermentation surfactant, including component A, B component and component C；
The component A includes the raw material of following parts by weight：Bacillus/55 parts of glutamic acid fermentation Product filtrate, go from
Sub- 18 parts of water, 0.08 part of Sodium Hyaluronate, 2.5 parts of glycerine, 3 parts of glycerine polymethacrylates, 1.5 parts of diglycerol, EDTA bis-
0.05 part of sodium；
The B component includes the raw material of following parts by weight：3 parts of avenabeta glucosan, 4 parts of edelweiss extract,
Purple 1 part of ball algae extract, 0.001 part of oligopeptides -1,3 parts of citrus extracts；
The component C includes the raw material of following parts by weight：4 parts of rose extract, 2 parts of glycerine, 1,2- pentanediols 0.1
Part, 0.2 part of Sensiva SC50.
Above-mentioned bacillus/glutamic acid fermentation Product filtrate is prepared by the following method to obtain：
(1) LB culture mediums are sterilized 35min under conditions of pressure is 0.01MPa and temperature is 118 DEG C, then by gemma
Bacillus strain is accessed in gnotobasis in the LB culture mediums after sterilizing, is subsequently placed in that temperature is 32 DEG C and rotating speed is 160rpm's
Shaking table culture 17h is carried out in shaking table, obtains seed liquor.
(2) seed liquor access is equipped with the fermentation tank of 100L fluid nutrient mediums by 1% inoculum concentration, is 32 in temperature
DEG C, throughput is the 60h that ferments under the stir speed (S.S.) of 100vvm and 200rpm, obtains fermentation of bacillus liquid, the Liquid Culture
Base includes following components in percentage by weight：Dusty yeast 1%, ammonium chloride 1.5%, sodium glutamate 9%, disodium hydrogen phosphate
0.15%th, the water of potassium dihydrogen phosphate 0.05%, sodium chloride 1.8%, glucose 3% and surplus.
(3) use continuous stream tube centrifuge by zymotic fluid through collected after centrifugation supernatant, then using hollow-fibre membrane
Supernatant is concentrated by ultrafiltration in filtration system, then concentrate after ultrafiltration is carried out autoclave sterilization, and sterilising conditions are set as：
118 DEG C of temperature, pressure 0.01MPa and time are 30min, obtain bacillus/glutamic acid fermentation Product filtrate.
The preparation method of above-mentioned biofermentation surfactant is as follows：
(1) main cooker is cleaned up and sterilized before producing, checks whether raw material is correct before feeding intake.
(2) first the Sodium Hyaluronate in component A is scattered in glycerine, each raw material in component A is then added into main cooker
Middle mixing, opens stirring, stir speed (S.S.) 400rpm；Then main cooker is warming up to 82 DEG C, homogeneous 4min, then keeps the temperature 22min and go out
Bacterium, whole process keep the stir speed (S.S.) of 400rpm；
(3) it is 300rpm to adjust stir speed (S.S.), main cooker is cooled to each raw material stirring added at 45 DEG C in B component
Mixing；
(4) each raw material for continuing to make main cooker add in component C when being cooled to 43 DEG C is stirred, then homogeneous 4min, after
Stop stirring after continuous stirring 32min, examine, discharging, obtain biofermentation surfactant.
Embodiment 3
A kind of biofermentation surfactant, including component A, B component and component C；
The component A includes the raw material of following parts by weight：Bacillus/60 parts of glutamic acid fermentation Product filtrate, water 18
Part, 0.12 part of Sodium Hyaluronate, 1 part of glycerine, 2 parts of glycerine polymethacrylates, 3 parts of diglycerol, 0.06 part of EDETATE SODIUM；
The B component includes the raw material of following parts by weight：3 parts of avenabeta glucosan, 3 parts of edelweiss extract,
Purple 2 parts of ball algae extract, 0.0012 part of oligopeptides -1,2 parts of citrus extracts；
The component C includes the raw material of following parts by weight：5 parts of rose extract, 3 parts of glycerine, 1 part of 1,2- pentanediols,
0.3 part of Sensiva SC50.
Above-mentioned bacillus/glutamic acid fermentation Product filtrate is prepared by the following method to obtain：
(1) LB culture mediums are sterilized 30min under conditions of pressure is 0.01MPa and temperature is 120 DEG C, then by gemma
Bacillus strain is accessed in gnotobasis in the LB culture mediums after sterilizing, is subsequently placed in that temperature is 35 DEG C and rotating speed is 170rpm's
Shaking table culture 16h is carried out in shaking table, obtains seed liquor.
(2) seed liquor access is equipped with the fermentation tank of 200L fluid nutrient mediums by 1% inoculum concentration, is 35 in temperature
DEG C, throughput is the 50h that ferments under the stir speed (S.S.) of 200vvm and 300rpm, obtains fermentation of bacillus liquid, the Liquid Culture
Base includes following components in percentage by weight：Dusty yeast 2%, ammonium chloride 2%, sodium glutamate 8%, disodium hydrogen phosphate 0.25%,
Potassium dihydrogen phosphate 0.1%, sodium chloride 1.5%, the water of glucose 4% and surplus.
(3) use continuous stream tube centrifuge by zymotic fluid through collected after centrifugation supernatant, then using hollow-fibre membrane
Supernatant is concentrated by ultrafiltration in filtration system, then concentrate after ultrafiltration is carried out autoclave sterilization, and sterilising conditions are set as：
120 DEG C of temperature, pressure 0.01MPa and time are 30min, obtain bacillus/glutamic acid fermentation Product filtrate.
The preparation method of above-mentioned biofermentation surfactant is as follows：
(1) main cooker is cleaned up and sterilized before producing, checks whether raw material is correct before feeding intake.
(2) first the Sodium Hyaluronate in component A is scattered in glycerine, each raw material in component A is then added into main cooker
Middle mixing, opens stirring, stir speed (S.S.) 600rpm；Then main cooker is warming up to 84 DEG C, homogeneous 5min, then keeps the temperature 30min and go out
Bacterium, whole process keep the stir speed (S.S.) of 600rpm；
(3) it is 400rpm to adjust stir speed (S.S.), main cooker is cooled to each raw material stirring added at 45 DEG C in B component
Mixing；
(4) each raw material for continuing to make main cooker add in component C when being cooled to 43 DEG C is stirred, then homogeneous 5min, after
Stop stirring after continuous stirring 40min, examine, discharging, obtain biofermentation surfactant.
Embodiment 4
A kind of biofermentation surfactant, including component A, B component and component C；
The component A includes the raw material of following parts by weight：Bacillus/65 parts of glutamic acid fermentation Product filtrate, go from
Sub- 22 parts of water, 0.15 part of Sodium Hyaluronate, 1.5 parts of glycerine, 1 part of glycerine polymethacrylates, 3 parts of diglycerol, EDETATE SODIUM
0.07 part；
The B component includes the raw material of following parts by weight：5 parts of avenabeta glucosan, 2 parts of edelweiss extract,
Purple 3 parts of ball algae extract, 0.0015 part of oligopeptides -1,1 part of citrus extracts；
The component C includes the raw material of following parts by weight：6 parts of rose extract, 4 parts of glycerine, 2 parts of 1,2- pentanediols,
0.4 part of Sensiva SC50.
Above-mentioned bacillus/glutamic acid fermentation Product filtrate is prepared by the following method to obtain：
(1) LB culture mediums are sterilized 25min under conditions of pressure is 0.01MPa and temperature is 122 DEG C, then by gemma
Bacillus strain is accessed in gnotobasis in the LB culture mediums after sterilizing, is subsequently placed in that temperature is 38 DEG C and rotating speed is 180rpm's
Shaking table culture 15h is carried out in shaking table, obtains seed liquor.
(2) seed liquor access is equipped with the fermentation tank of 300L fluid nutrient mediums by 8% inoculum concentration, is 38 in temperature
DEG C, throughput is the 30h that ferments under the stir speed (S.S.) of 300vvm and 500rpm, obtains fermentation of bacillus liquid, the Liquid Culture
Base includes following components in percentage by weight：Dusty yeast 2.5%, ammonium chloride 2.5%, sodium glutamate 6%, disodium hydrogen phosphate
0.25%th, the water of potassium dihydrogen phosphate 0.12%, sodium chloride 1.2%, glucose 4% and surplus.
(3) use continuous stream tube centrifuge by zymotic fluid through collected after centrifugation supernatant, then using hollow-fibre membrane
Supernatant is concentrated by ultrafiltration in filtration system, then concentrate after ultrafiltration is carried out autoclave sterilization, and sterilising conditions are set as：
122 DEG C of temperature, pressure 0.01MPa and time are 25min, obtain bacillus/glutamic acid fermentation Product filtrate.
The preparation method of above-mentioned biofermentation surfactant is as follows：
(1) main cooker is cleaned up and sterilized before producing, checks whether raw material is correct before feeding intake.
(2) first the Sodium Hyaluronate in component A is scattered in glycerine, each raw material in component A is then added into main cooker
Middle mixing, opens stirring, stir speed (S.S.) 700rpm；Then main cooker is warming up to 84 DEG C, homogeneous 4min, then keeps the temperature 25min and go out
Bacterium, whole process keep the stir speed (S.S.) of 700rpm；
(3) it is 300rpm to adjust stir speed (S.S.), main cooker is cooled to each raw material stirring added at 45 DEG C in B component
Mixing；
(4) each raw material for continuing to make main cooker add in component C when being cooled to 43 DEG C is stirred, then homogeneous 4min, after
Stop stirring after continuous stirring 40min, examine, discharging, obtain biofermentation surfactant.
Embodiment 5
A kind of biofermentation surfactant, including component A, B component and component C；
The component A includes the raw material of following parts by weight：Bacillus/70 parts of glutamic acid fermentation Product filtrate, go from
Sub- 25 parts of water, 0.2 part of Sodium Hyaluronate, 1 part of glycerine, 1 part of glycerine polymethacrylates, 3.5 parts of diglycerol, EDETATE SODIUM
0.08 part；
The B component includes the raw material of following parts by weight：5 parts of avenabeta glucosan, 1 part of edelweiss extract,
Purple 3.5 parts of ball algae extract, 0.002 part of oligopeptides -1,1 part of citrus extracts；
The component C includes the raw material of following parts by weight：4 parts of rose extract, 4 parts of glycerine, 2 parts of 1,2- pentanediols,
0.5 part of Sensiva SC50.
Above-mentioned bacillus/glutamic acid fermentation Product filtrate is prepared by the following method to obtain：
(1) LB culture mediums are sterilized 30min under conditions of pressure is 0.01MPa and temperature is 120 DEG C, then by gemma
Bacillus strain is accessed in gnotobasis in the LB culture mediums after sterilizing, is subsequently placed in that temperature is 35 DEG C and rotating speed is 200rpm's
Shaking table culture 15h is carried out in shaking table, obtains seed liquor.
(2) seed liquor access is equipped with the fermentation tank of 400L fluid nutrient mediums by 1% inoculum concentration, is 32 in temperature
DEG C, throughput is the 24h that ferments under the stir speed (S.S.) of 400vvm and 700rpm, obtains fermentation of bacillus liquid, the Liquid Culture
Base includes following components in percentage by weight：Dusty yeast 3%, ammonium chloride 2.5%, sodium glutamate 5%, disodium hydrogen phosphate 0.3%,
Potassium dihydrogen phosphate 0.15%, sodium chloride 1%, the water of glucose 5% and surplus.
(3) use continuous stream tube centrifuge by zymotic fluid through collected after centrifugation supernatant, then using hollow-fibre membrane
Supernatant is concentrated by ultrafiltration in filtration system, then concentrate after ultrafiltration is carried out autoclave sterilization, and sterilising conditions are set as：
118 DEG C of temperature, pressure 0.01MPa and time are 35min, obtain bacillus/glutamic acid fermentation Product filtrate.
The preparation method of above-mentioned biofermentation surfactant is as follows：
(1) main cooker is cleaned up and sterilized before producing, checks whether raw material is correct before feeding intake.
(2) first the Sodium Hyaluronate in component A is scattered in glycerine, each raw material in component A is then added into main cooker
Middle mixing, opens stirring, stir speed (S.S.) 400rpm；Then main cooker is warming up to 80 DEG C, homogeneous 3min, then keeps the temperature 20min and go out
Bacterium, whole process keep the stir speed (S.S.) of 400rpm；
(3) it is 200rpm to adjust stir speed (S.S.), main cooker is cooled to each raw material stirring added at 45 DEG C in B component
Mixing；
(4) each raw material for continuing to make main cooker add in component C when being cooled to 43 DEG C is stirred, then homogeneous 3min, after
Stop stirring after continuous stirring 35min, examine, discharging, obtain biofermentation surfactant.
The preferred embodiment of the present invention is above are only, is not intended to limit the invention, for those skilled in the art
For member, inventive formulation and preparation process can have various modifications and variations.Within the spirit and principles of the invention, institute
Any modification, equivalent substitution, improvement and etc. of work, should all be included in the protection scope of the present invention.
Claims (10)
- A kind of 1. biofermentation surfactant, it is characterised in that：Including component A, B component and component C；The component A includes the raw material of following parts by weight：Bacillus/50~70 parts of glutamic acid fermentation Product filtrate, water 15 ~25 parts, 0.05~0.2 part of Sodium Hyaluronate, 1~3 part of glycerine, 1~4 part of glycerine polymethacrylates, diglycerol 1~3.5 Part, 0.04~0.08 part of EDETATE SODIUM；The B component includes the raw material of following parts by weight：2~5 parts of avenabeta glucosan, edelweiss extract 1~4.5 Part, purple 0.5~3.5 part of ball algae extract, 0.0005~0.002 part of oligopeptides -1,1~4 part of citrus extracts；The component C includes the raw material of following parts by weight：1~4 part of glycerine, 0.05~2 part of 1,2- pentanediols, ethylhexyl are sweet 0.1~0.5 part of oil；The bacillus/glutamic acid fermentation Product filtrate is prepared by the following method to obtain：(1) Bacillus strain is accessed in gnotobasis in sterilized LB culture mediums, carries out shaking table culture, obtain seed Liquid；(2) seed liquor is accessed in fermentation tank by the inoculum concentration of 1~10vol% and fermented, obtain fermentation of bacillus Liquid；(3) zymotic fluid is taken into supernatant after centrifugation, is then concentrated by ultrafiltration, obtain bacillus/glutamic acid fermentation Product filtrate.
- 2. biofermentation surfactant according to claim 1, it is characterised in that：The component C further includes following parts by weight Several raw materials：3~6 parts of floral type plant extracts.
- 3. biofermentation surfactant according to claim 1, it is characterised in that：During the shaking table culture：Shaking table temperature Spend for 30~40 DEG C, shaking speed is 150~200rpm, and incubation time is 15~18h.
- 4. biofermentation surfactant according to claim 1, it is characterised in that：50~400L is equipped with the fermentation tank Fluid nutrient medium after sterilized, the fluid nutrient medium include following components in percentage by weight：Dusty yeast 0.5~3%, chlorine Change ammonium 1~2.5%, sodium glutamate 5~10%, disodium hydrogen phosphate 0.1~0.3%, potassium dihydrogen phosphate 0.02~0.15%, chlorination The water of sodium 1~2%, glucose 2~5% and surplus.
- 5. biofermentation surfactant according to claim 4, it is characterised in that：The sterilization process includes：It is in pressure 20~40min of sterilizing under conditions of 0.01MPa and temperature are 115~122 DEG C.
- 6. biofermentation surfactant according to claim 1, it is characterised in that：The fermentation process includes：It is in temperature 30~40 DEG C, throughput is the 24~64h that ferments under the stir speed (S.S.) of 50~400vvm and 100~700rpm.
- 7. the preparation method of biofermentation surfactant according to claim 1, it is characterised in that：In step (3), described Further included after ultrafiltration concentration：By obtained ultrafiltration concentration liquid under conditions of pressure is 0.01MPa and temperature is 115~122 DEG C Sterilize 20~40min.
- 8. biofermentation surfactant according to claim 2, it is characterised in that：The floral type plant extracts is rose Flower extract, Herba Lysimachiae foenumgraeci extract or orchid extract.
- A kind of 9. preparation method of biofermentation surfactant, it is characterised in that：The biofermentation surfactant for claim 1~ 8 any one of them biofermentation surfactant, the preparation method include：(1) first the Sodium Hyaluronate in component A is scattered in glycerine, then each raw material in component A is added in main cooker and is stirred Mix uniformly mixed；When main cooker is warming up to 80~84 DEG C, 3~5min of homogeneous, then keep the temperature 20~25min sterilizings；(2) it is stirred each raw material that main cooker is added in B component when being cooled to 45 DEG C；(3) each raw material for continuing to make main cooker add in component C when being cooled to 43 DEG C is stirred, then 3~5min of homogeneous, is continued Stir 30~40min.
- 10. the preparation method of biofermentation surfactant according to claim 9, it is characterised in that：The stir speed (S.S.) is 100~700rpm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810070935.5A CN107970203A (en) | 2018-01-25 | 2018-01-25 | A kind of biofermentation surfactant and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810070935.5A CN107970203A (en) | 2018-01-25 | 2018-01-25 | A kind of biofermentation surfactant and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107970203A true CN107970203A (en) | 2018-05-01 |
Family
ID=62006240
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810070935.5A Pending CN107970203A (en) | 2018-01-25 | 2018-01-25 | A kind of biofermentation surfactant and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107970203A (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108619078A (en) * | 2018-08-20 | 2018-10-09 | 广州无添加主义化妆品有限公司 | A kind of whitening skin lightening facial mask and preparation method thereof |
| CN109528555A (en) * | 2019-01-11 | 2019-03-29 | 广东双骏生物科技有限公司 | A kind of multidimensional fermentation Essence/powder and its preparation method and application |
| CN109620775A (en) * | 2019-01-30 | 2019-04-16 | 昆明贝泰妮生物科技有限公司 | One kind repairing red composition, compound formulation and preparation method thereof |
| CN111888293A (en) * | 2020-08-19 | 2020-11-06 | 上海怡宝化妆品集团有限公司 | Moisturizing toner containing rose fermentation liquor and preparation method thereof |
| CN111991335A (en) * | 2020-08-27 | 2020-11-27 | 广州优科生物科技有限公司 | Preparation method of anti-aging bacillus fermentation product |
| CN114748406A (en) * | 2022-05-12 | 2022-07-15 | 杭州孔凤春化妆品股份有限公司 | Soothing and moisturizing skin moisturizer and preparation method thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20090041814A1 (en) * | 2007-08-07 | 2009-02-12 | Aska Corporation Co. Ltd. | Cosmetic composition |
| CN106176276A (en) * | 2016-09-27 | 2016-12-07 | 广东丸美生物技术股份有限公司 | A kind of have defying age and the skin matrix of antiallergic effect and preparation and application thereof |
-
2018
- 2018-01-25 CN CN201810070935.5A patent/CN107970203A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20090041814A1 (en) * | 2007-08-07 | 2009-02-12 | Aska Corporation Co. Ltd. | Cosmetic composition |
| CN106176276A (en) * | 2016-09-27 | 2016-12-07 | 广东丸美生物技术股份有限公司 | A kind of have defying age and the skin matrix of antiallergic effect and preparation and application thereof |
Non-Patent Citations (2)
| Title |
|---|
| 广州无添加主义化妆品有限公司: "《http://125.35.6.80:8181/ftban/itownet/hzp_ba/fw/pz.jsp?processid=20180504081203jteoa&nid=20180504081203jteoa》", 9 July 2017, 国家非特殊用途化妆品备案电子凭证 * |
| 张文等: "纳豆芽孢杆菌(Bacillus natto)发酵生产γ-聚谷氨酸过程中培养基组分的优化", 《中国生物工程杂志》 * |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108619078A (en) * | 2018-08-20 | 2018-10-09 | 广州无添加主义化妆品有限公司 | A kind of whitening skin lightening facial mask and preparation method thereof |
| CN109528555A (en) * | 2019-01-11 | 2019-03-29 | 广东双骏生物科技有限公司 | A kind of multidimensional fermentation Essence/powder and its preparation method and application |
| CN109620775A (en) * | 2019-01-30 | 2019-04-16 | 昆明贝泰妮生物科技有限公司 | One kind repairing red composition, compound formulation and preparation method thereof |
| CN111888293A (en) * | 2020-08-19 | 2020-11-06 | 上海怡宝化妆品集团有限公司 | Moisturizing toner containing rose fermentation liquor and preparation method thereof |
| CN111888293B (en) * | 2020-08-19 | 2022-08-16 | 上海洁士宝日化集团有限公司 | Moisturizing toner containing rose fermentation liquor and preparation method thereof |
| CN111991335A (en) * | 2020-08-27 | 2020-11-27 | 广州优科生物科技有限公司 | Preparation method of anti-aging bacillus fermentation product |
| CN114748406A (en) * | 2022-05-12 | 2022-07-15 | 杭州孔凤春化妆品股份有限公司 | Soothing and moisturizing skin moisturizer and preparation method thereof |
| CN114748406B (en) * | 2022-05-12 | 2024-01-19 | 杭州孔凤春化妆品股份有限公司 | Skin care lotion capable of relieving and preserving moisture and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN107970203A (en) | A kind of biofermentation surfactant and preparation method thereof | |
| CN1761450B (en) | Skin preparation for external use characterized by containing sugar derivative of a,a-trehalose | |
| CN102112106B (en) | Cosmetic composition for anti-aging of the skin comprising phaseolus radiatus seed extracts by fermentation and enzyme treatment | |
| CN105125476A (en) | Anti-wrinkle essence and preparing method thereof | |
| CN109170469A (en) | A kind of Isin glue collagen peptide solid beverage and preparation method thereof | |
| CN107898660A (en) | A kind of tender skin water light stoste and its preparation method and application | |
| CN109199964A (en) | One kind to peptide quintessence dew and preparation method thereof | |
| CN109998986A (en) | One kind two splits yeast freeze-dried powder preparation and preparation method thereof | |
| CN107260571A (en) | Protein skin care item with skin repair and care action | |
| CN105796474A (en) | Solid multifunctional mask composition and method for preparing mask | |
| CN108158971A (en) | A kind of ginseng U.S. face film and preparation method | |
| CN107854353A (en) | A kind of camellia skin-protection Essence and its preparation technology | |
| CN108186432A (en) | The facial mask and preparation method of raw material are wanted based on a kind of cape jasmine flower extract | |
| CN108078854B (en) | Hand frost and preparation method thereof is repaired in a kind of moisturizing | |
| CN109939064A (en) | The Essence of the metabolite containing probiotics fermention and its application in cosmetics | |
| CN113842341B (en) | Roselle fermentation product and preparation method and application thereof | |
| JP2008120792A (en) | Skin and hair cosmetic having moisture retention and hairdressing activity and method for producing the same | |
| CN106176327A (en) | A kind of shampoo containing polyglutamic acid and preparation method thereof | |
| CN105263471B (en) | Moisturizer | |
| CN112675060B (en) | Composition and skin care product for resisting skin aging and preparation method thereof | |
| CN108888554A (en) | Stem cell beauty lotion and preparation method thereof with the light spot of tender skin | |
| CN107397712A (en) | A kind of snake gall facial mask and preparation method thereof | |
| CN108635272A (en) | A kind of anti-age corrective eye treatment cream and preparation method thereof | |
| CN109010199A (en) | A kind of skin whitening, moisturizing composition and its preparation method and application | |
| CN104306322A (en) | Cordyceps-militaris-ingredient contained eye cream and preparing method of cordyceps-militaris-ingredient contained eye cream |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20180501 |