CN109528555A - A kind of multidimensional fermentation Essence/powder and its preparation method and application - Google Patents
A kind of multidimensional fermentation Essence/powder and its preparation method and application Download PDFInfo
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- CN109528555A CN109528555A CN201910024963.8A CN201910024963A CN109528555A CN 109528555 A CN109528555 A CN 109528555A CN 201910024963 A CN201910024963 A CN 201910024963A CN 109528555 A CN109528555 A CN 109528555A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/84—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds obtained by reactions otherwise than those involving only carbon-carbon unsaturated bonds
- A61K8/88—Polyamides
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/67—Vitamins
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/85—Products or compounds obtained by fermentation, e.g. yoghurt, beer, wine
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- Life Sciences & Earth Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Birds (AREA)
- Epidemiology (AREA)
- Dermatology (AREA)
- Gerontology & Geriatric Medicine (AREA)
- Cosmetics (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The application discloses a kind of multidimensional fermentation Essence, as mass fraction, the folic acid of the farnoquinone of levulan LEVAN, 0.2ppm-94ppm of polyglutamic acid, 0.01%-15.6% comprising 0.1%-30%, 0.02ppm-32ppm, wherein the molecular weight of the polyglutamic acid is 400D-3,000,000D.The application also provides the preparation method of the multidimensional fermentation Essence, and the preparation method includes: the preparation of bacillus subtilis fermentation liquor；The bacillus subtilis fermentation liquor is adjusted to pH 3.9-4.5 with acid；Diatomite is added, standing sedimentation discards precipitating；The supernatant of acquisition is filtered by film, removes protein and salt, and wash decoloration, obtains the multidimensional fermentation Essence.A kind of cosmetics are also disclosed in the application, include multidimensional fermentation Essence or multidimensional fermentation essence powder.
Description
Technical field
This application involves cosmetic field more particularly to a kind of multidimensional fermentation Essence/powder preparation method and applications.
Background technique
The functional raw material of cosmetics mainly includes beauty Chinese medicine, biological products, natural function raw material.Beauty Chinese medicine is simultaneous
Have nutrition and drug effect double action, there is certain advantage compared to the raw material for playing synthesis, but the ingredient of beauty Chinese medicine is relative complex,
Just there are several kinds of even tens kinds of ingredients inside a herb, it is less with regard to relative amount for the ingredient for improving skin.With change
Active principle is extracted discharging by method may then remain other useless organic solvents.And part Chinese medicine be it is toxic, than
Such as Chinese blister beetle.Some Chinese medicines are containing carcinogenic volatile oil, such as asarum, cloves.And the research to the ingredient in Chinese medicine, in existing rank
Section be it is incomplete, we not can determine that the pure natural Chinese medicine of addition is entirely without harmful effect to our skin.
The cosmetic material of biological products is greatly mostly from animal, and the virus that may be carried with animal is to the safety of cosmetic material
Property brings a uncertainty.And the cosmetic material of biological products on the market is due to material source and extracting method, valence
Lattice are relatively high.Although natural function raw material is bragged about naturally, but test run is chemical during the extraction process for most of raw material
Method is extracted using some organic solvents, it is possible to lead to the residual of organic solvent.
Summary of the invention
It is the general introduction to the theme being described in detail herein below.This general introduction is not the protection model in order to limit claim
It encloses.
The research of bacillus subtilis and the history of existing more than 100 years of application, as people study bacillus subtilis
Carry out in a deep going way, find its industry, agricultural, medical and health, health care of food, in terms of have extensive use valence
Value.Bacillus subtilis enzyme is one of current widest strain of industrial enzyme production application, and the enzyme of fermenting and producing is in food, feeding
Material, washing, weaving, leather, papermaking and medicine and other fields play highly important effect.And it is sent out by bacillus subtilis
The cosmetic material source that ferment is got understands, not by chemical synthesis, and the method for obtaining raw material is by advanced membrane technology
It is handled, does not add organic solvent, veritably to skin fanout free region without toxic action.
It ferments Essence/powder this application provides a kind of multidimensional, multidimensional fermentation Essence/powder, which has, to be obviously increased
Skin whitening, moisturizing, dewrinkling spot-eliminating effect.
Specifically, this application provides a kind of multidimensional fermentation Essences, as mass fraction, poly- comprising 0.1%-30%
Glutamic acid, the farnoquinone of levulan LEVAN, 0.2ppm-94ppm of 0.01%-15.6%, 0.02ppm-32ppm folic acid,
Ppm indicate concentration unit, microgram, wherein the molecular weight of the polyglutamic acid be 400D-3,000,000D.Fruit is poly-
Sugared LEVAN is the polysaccharide for containing a large amount of β-(2,6) glycosidic bond and a small amount of β-(2,1) fructose glycosidic bond in molecule.
In this application, wherein as mass fraction, the multidimensional fermentation Essence includes the polyglutamic of 0.1%-30%
The folic acid of acid, the LEVE polysaccharide of 4%-10%, the farnoquinone of 40ppm-94ppm, 0.02ppm-32ppm, ppm indicate concentration
Unit, microgram, wherein the molecular weight of the polyglutamic acid is 2,000-1,000,000D.
The application also provides the preparation method of the multidimensional fermentation Essence, and the preparation method includes:
(1) the bacillus subtilis bacteria suspension that concentration is 5%-20% the preparation of bacillus subtilis fermentation liquor: is put into dress
It spreads cultivation in the seeding tank for having LB liquid, after seed liquor concentration reaches bacterium of the OD600 between 1.2-2.2 amount living, investment dress
Have in the fermentor of fermentation liquid, fermentation -48 hours 10 hours is carried out at 35 DEG C -50 DEG C, obtains the fermentation of bacillus subtilis
Liquid；
(2) bacillus subtilis fermentation liquor is adjusted to pH 3.9-4.5 with acid；
(3) diatomite is added, standing sedimentation discards precipitating；
(4) supernatant obtained in step (3) is filtered by film, removes protein and salt, and wash decoloration, obtains institute
State multidimensional fermentation Essence.
In this application, in step (1), the bacillus subtilis separates from natto and obtains, and is wild type natto
Bacillus subtilis bacterial spawn (Bacillus subtilis natto) ST188, CGMCC No.8400.
In this application, in step (1), LB liquid is Luria-Bertani fluid nutrient medium: pressing mass volume ratio g/
100ml water meter includes 0.1% glucose, 1% yeast powder, 0.5% tryptone, 0.05% sodium chloride.
In this application, in step (1), the fermentation liquid includes 2% glucose, 1% glycerol, 3% soybean powder, 1%
Glutamic acid.
In this application, wherein in step (2), the acid can be any one of hydrochloric acid, sulfuric acid, acetic acid or more
It is a variety of.
In this application, wherein in step (3), the quality of the diatomite and the bacillus subtilis after adjusting pH
The ratio between volume of fermented liquid can be 1g/ml-3g/ml.
In this application, wherein in step (3), can be settled at 0 DEG C -35 DEG C.
In this application, wherein in step (3), can settle 6-12 hours.
In this application, wherein in step (3), can be settled 6-12 hours at 0 DEG C -35 DEG C.
In this application, wherein in step (4), the film may include ceramic membrane and/or hollow-fibre membrane, wherein
The aperture of the film is 0.1 μm -1 μm.
The application also provides a kind of multidimensional fermentation essence powder: the polyglutamic acid of 50%-95%,
The folic acid of the farnoquinone of levulan LEVEN, 100ppm-300ppm of 5%-50%, 10ppm-100ppm, ppm indicate concentration
Unit, microgram, wherein the molecular weight of the polyglutamic acid be 400D-3,000,000D.
In this application, as mass fraction, multidimensional fermentation essence powder include 50%-80% polyglutamic acid,
The folic acid of the farnoquinone of levulan LEVAN, 100ppm-300ppm of 5%-20%, 10ppm-100ppm, ppm indicate concentration
Unit, microgram, wherein the molecular weight of the polyglutamic acid be 400D-1200,000D.
The application also provides the preparation method of the multidimensional fermentation essence powder, and the preparation method includes: by the multidimensional
The Essence that ferments passes through alcohol precipitation, and dry, obtains the multidimensional fermentation essence powder.
In this application, wherein the alcohol can be selected from any one of methanol, ethyl alcohol, propyl alcohol or more, the alcohol
Volume can for the multidimensional ferment Essence volume 50%-80%.
The application also provides a kind of facial mask, includes multidimensional fermentation Essence or multidimensional fermentation essence powder.
The multidimensional fermentation Essence and multidimensional fermentation essence powder of the application, hence it is evident that increase the aqueous degree of skin, spot desalinates journey
Degree, texture compactness, pore visibility have skin whitening, moisturizing, the effect of dewrinkling spot-eliminating.
Other features and advantage will illustrate in the following description, also, partly become from specification
It obtains it is clear that being understood and implementing the application.The purpose of the application and other advantages can be by specifications, right
Specifically noted structure is achieved and obtained in claim.
Specific embodiment
It, hereinafter will be to embodiments herein for the purposes, technical schemes and advantages of the application are more clearly understood
It is described in detail.It should be noted that in the absence of conflict, the features in the embodiments and the embodiments of the present application can
With mutual any combination.
Embodiment 1
The preparation of bacillus subtilis fermentation liquor:
Bacillus subtilis natto is cultivated 24 hours in LB solid slope culture medium, is then prepared into 5L sterile water
Bacteria suspension.By 5L bacillus subtilis bacteria suspension investment equipped with the 3.5h that spreads cultivation is carried out in the seeding tank of 160LB liquid, to seed liquor concentration
After reaching bacterium of the OD600 between 1.2-2.2 amount living, then putting into 2T, (fermentation liquid includes 2% grape equipped with fermentation liquid 1.4T
Sugar, 1% glycerol, 3% soybean powder, 1% glutamic acid) fermentor in carry out fermentation 23h, during which carry out feed supplement supplement, make bacterium
Body is in high activity state during the fermentation, and assembles the metabolite high concentration in fermentation liquid.
The preparation of multidimensional fermentation Essence:
By bacillus subtilis fermentation liquor, it is 3.90 with 6N salt acid for adjusting pH, 2% diatomite is added after mixing evenly, sink
Drop 12 hours takes supernatant to take off albumen, salt and pigment by ceramic membrane, and is concentrated to get Essence.
Embodiment 2
The preparation of multidimensional fermentation essence powder:
By bacillus subtilis fermentation liquor, it is 3.90 with 6N sulphur acid for adjusting pH, 1.5% diatomite is added, after mixing evenly,
Sedimentation 12 hours takes supernatant to take off albumen, salt and pigment by ceramic membrane, and is concentrated to get Essence, heavy using 50% ethyl alcohol
Afterwards, it is dried in vacuo to obtain multidimensional fermentation essence powder.
Performance test
Essence is added auxiliary material and facial mask is made, and essence powder 4%-10% in mass ratio is dissolved in water, emulsification, filling, packaging,
Facial mask is made, experiences through instrument detection and face, is as a result shown in Tables 1 and 2.Detection method: the application product the 7th is used
It, the 14th day, with skin skin quality analyzer (Canfield company, the U.S., VISIA) measure eight large projects such as spot, ultraviolet light spot
Point, brown spot, general red area, wrinkle, sebum secretion amount, number of pores, skin smoothness；Multi-functional skin quality is recycled to detect
Instrument dark red pigment probe (MX18), elastic force probe (CT-MPA580) surveys its skin melanin, red
Element, elastic force, four project of moisturizing degree amount to the detection of 12 projects.Before tested, subject washes one's face in advance, rest after twenty minutes, into
Enter laboratory, with skin analyzer, Multi-functional skin analyzer, Multi-functional skin analyzer test subject facial skin number
Value (test zone are as follows: right cheek, left cheek, forehead totally three region.Every subject needs sooner or later to produce the application during the experiment
Product are coated on the face.It the 7th day, the 14th day after use, returns in laboratory, carries out using the application product once again in two times
Skin test afterwards.The interference of environment is excluded in test process).Test is tested using skin detector, and mode is tester
After clean face of first removing ornaments and formal dress, under full face element face state, first measure be not used facial mask before skin condition (the aqueous degree of skin, spot amount, tightly
Cause degree, pore visibility, wherein aqueous degree is test moisture-retaining capacity；Spot amount is test nti-freckle ability；Compact degree is that test is tight
Skin ability；Pore visibility is test pore refining ability), it is applied to face using Essence later, after 15 minutes, then does flesh
Skin detection, the reference data contrasted using test front and back, and the cross-reference numerical value of 20 people is done and averagely obtains the above number
According to.Therefore failing the unit data of spottiness amount, compact degree, pore visibility, tester is only capable of testing out front and back correlation data.
VISIA skin detection instrument uses optical imagery, RBX and software science and technology, measures and analyze immediately spot, the hair of epidermis
Hole, wrinkle and dermatoglyph, and the veins beneath the skin generated and pigmentosa lesion due to ultraviolet light irradiation, such as porphyrin (oil
Rouge), moth patch, erythema etc., and disclose such as chloasma, acne, brandy nose and spider shape phlebangioma as caused by them
Potential danger.
Table 1 includes the testing result of the facial mask of multidimensional fermentation Essence
Table 2 includes the testing result of the facial mask of multidimensional fermentation essence powder
| Tested number | 20 people |
| The aqueous degree of skin | Averagely increase by 15% |
| Spot desalinates degree | The averagely spot amount of desalination 6% |
| The compact degree of texture | Averagely increase 9% compact degree |
| Pore visibility | Averagely reduce by the 9% compact degree of pore |
As can be seen from Table 1 and Table 2, in the face using the multidimensional fermentation Essence comprising the application or multidimensional essence powder
After film, the aqueous degree of skin, spot desalination degree, texture compactness, pore visibility are obviously increased, and illustrate the application's
Multidimensional fermentation liquid and multidimensional yeast powder have the function of skin whitening, moisturizing, dewrinkling spot-eliminating.
Although embodiment disclosed by the application is as above, the content only for ease of understanding the application and use
Embodiment is not limited to the application.Technical staff in any the application fields, is taken off not departing from the application
Under the premise of the spirit and scope of dew, any modification and variation, but the application can be carried out in the form and details of implementation
Scope of patent protection, still should be subject to the scope of the claims as defined in the appended claims.
Claims (11)
- Essence, as mass fraction, polyglutamic acid, 0.01%-15.6% comprising 0.1%-30% 1. a kind of multidimensional is fermented The farnoquinone of levulan LEVAN, 0.2ppm-94ppm, 0.02ppm-32ppm folic acid, ppm indicates the unit of concentration, hundred A ten thousandth gram, wherein the molecular weight of the polyglutamic acid be 400D-3,000,000D.
- 2. the preparation method of multidimensional fermentation Essence described in claim 1, the preparation method include:(1) the bacillus subtilis bacteria suspension that mass concentration is 5%-20% the preparation of bacillus subtilis fermentation liquor: is put into dress It spreads cultivation in the seeding tank for having LB liquid, after seed liquor concentration reaches bacterium of the OD600 between 1.2-2.2 amount living, investment dress Have in the fermentor of fermentation liquid, fermentation -48 hours 10 hours is carried out at 35 DEG C -50 DEG C, obtains the fermentation of bacillus subtilis Liquid；(2) bacillus subtilis fermentation liquor is adjusted to pH 3.9-4.5 with acid；(3) diatomite is added, standing sedimentation discards precipitating；(4) supernatant obtained in step (2) is filtered by film, removes protein and salt, and wash decoloration, obtains described more Dimension fermentation Essence.
- 3. preparation method according to claim 2, wherein the bacillus subtilis is divided from natto in step (1) It is wild type Bacillus subtilis natto column (Bacillus subtilis natto) ST188, CGMCC from acquisition No.8400。
- 4. preparation method according to claim 2, wherein in step (2), the acid is hydrochloric acid, in sulfuric acid, acetic acid It is any or more.
- 5. preparation method according to claim 2, wherein in step (3), the quality of the diatomite and adjust pH it The ratio between volume of bacillus subtilis fermentation liquor afterwards is 1g/ml-3g/ml.
- 6. preparation method according to claim 2, wherein in step (3), settled 6-12 hours at 0 DEG C -35 DEG C.
- 7. preparation method according to claim 2, wherein in step (4), the film includes ceramic membrane and/or hollow Tunica fibrosa, wherein the aperture of the film is 0.1 μm -1 μm.
- 8. a kind of multidimensional fermentation essence powder includes as mass fraction: the polyglutamic acid of 50%-95%, the fruit of 5%-50% are poly- The folic acid of the farnoquinone of sugared LEVAN, 100ppm-300ppm, 10ppm-100ppm, ppm indicate the unit of concentration, million/ One gram, wherein the molecular weight of the polyglutamic acid be 400D-3,000,000D.
- 9. the preparation method of multidimensional fermentation essence powder according to any one of claims 8, the preparation method includes: by claim 1 institute The multidimensional fermentation Essence stated passes through alcohol precipitation, and dry, obtains the multidimensional fermentation essence powder.
- 10. preparation method according to claim 9, wherein using any of the alcohol in methanol, ethyl alcohol, propyl alcohol Kind or more, the volume of the alcohol is the 50%-80% of the volume of multidimensional fermentation Essence.
- 11. a kind of cosmetics include multidimensional described in claim 1 fermentation Essence or multidimensional according to any one of claims 8 fermentation Essence powder.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201910024963.8A CN109528555A (en) | 2019-01-11 | 2019-01-11 | A kind of multidimensional fermentation Essence/powder and its preparation method and application |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111544371A (en) * | 2020-06-12 | 2020-08-18 | 洋浦吉商生物科技有限公司 | Whitening and repairing mask containing probiotic active extracting solution and nicotinamide |
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| JPS5638125B2 (en) * | 1978-10-17 | 1981-09-04 | ||
| CN106389249A (en) * | 2016-09-18 | 2017-02-15 | 天津北洋百川生物技术有限公司 | Gamma-polyglutamic acid containing hydrating toner and preparation method thereof |
| CN107970203A (en) * | 2018-01-25 | 2018-05-01 | 广州无添加主义化妆品有限公司 | A kind of biofermentation surfactant and preparation method thereof |
| CN108926521A (en) * | 2017-05-19 | 2018-12-04 | 共荣化学工业株式会社 | Plant fermentation object |
| KR20190001416A (en) * | 2017-06-27 | 2019-01-04 | 주식회사 에이치앤비나인 | A cosmetic composition comprising fermented coffee grounds |
-
2019
- 2019-01-11 CN CN201910024963.8A patent/CN109528555A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5638125B2 (en) * | 1978-10-17 | 1981-09-04 | ||
| CN106389249A (en) * | 2016-09-18 | 2017-02-15 | 天津北洋百川生物技术有限公司 | Gamma-polyglutamic acid containing hydrating toner and preparation method thereof |
| CN108926521A (en) * | 2017-05-19 | 2018-12-04 | 共荣化学工业株式会社 | Plant fermentation object |
| KR20190001416A (en) * | 2017-06-27 | 2019-01-04 | 주식회사 에이치앤비나인 | A cosmetic composition comprising fermented coffee grounds |
| CN107970203A (en) * | 2018-01-25 | 2018-05-01 | 广州无添加主义化妆品有限公司 | A kind of biofermentation surfactant and preparation method thereof |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111544371A (en) * | 2020-06-12 | 2020-08-18 | 洋浦吉商生物科技有限公司 | Whitening and repairing mask containing probiotic active extracting solution and nicotinamide |
| CN111544371B (en) * | 2020-06-12 | 2020-11-20 | 洋浦吉商生物科技有限公司 | Whitening and repairing mask containing probiotic active extracting solution and nicotinamide |
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