CN107966423B - 一种基于锌的功能核酸的耐高盐比色传感器及其应用 - Google Patents
一种基于锌的功能核酸的耐高盐比色传感器及其应用 Download PDFInfo
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CN108841937B (zh) * | 2018-06-20 | 2020-07-28 | 中国农业大学 | 通用隔断超快扩增镁、锌切割型功能核酸可视化检测方法 |
CN109207570B (zh) * | 2018-09-30 | 2022-11-15 | 重庆医科大学 | 一种免DNA提取的快速SNPs基因分型新方法 |
CN109609607B (zh) * | 2018-12-25 | 2022-03-01 | 中国农业大学 | 一种用于锌离子定量检测的方法 |
CN113201582B (zh) * | 2021-04-01 | 2023-08-15 | 南京邮电大学 | 一种基于磁颗粒及等温核酸扩增方法组装的比色传感器及其制备方法和应用 |
CN114152599B (zh) * | 2021-12-01 | 2023-10-13 | 中国农业大学 | 一种基于双劈裂功能核酸变构的孔雀石绿生物传感器 |
CN114606295B (zh) * | 2022-01-26 | 2024-04-12 | 湖南大学 | 基于脱氧核糖核酶的去甲基化酶活性检测方法和应用 |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1850981A (zh) * | 2006-03-10 | 2006-10-25 | 杭州优思达生物技术有限公司 | 切口酶扩增靶核酸序列的方法及用于扩增靶核酸序列的试剂盒及其应用 |
CN105136875A (zh) * | 2015-07-31 | 2015-12-09 | 江苏巨珩新材料科技有限公司 | 利用超级三明治核酸探针纳米孔道传感器高灵敏高特异性检测Zn2+的方法 |
CN106337082A (zh) * | 2016-04-28 | 2017-01-18 | 湖南大学 | 基于酶法生成随机g四链体的细胞凋亡免标记检测方法 |
CN106520927A (zh) * | 2016-09-23 | 2017-03-22 | 广东省生态环境技术研究所 | 一种核酸检测方法及试剂盒 |
CN107012208A (zh) * | 2017-03-08 | 2017-08-04 | 广东省生态环境技术研究所 | 一种免标记铅离子可视化检测方法及检测试剂盒 |
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US7612185B2 (en) * | 2003-03-07 | 2009-11-03 | The Board Of Trustees Of The University Of Illinois | Nucleic acid biosensors |
CN102014902A (zh) * | 2005-01-06 | 2011-04-13 | 默克公司 | 检查点激酶抑制剂 |
US8933210B2 (en) * | 2010-10-06 | 2015-01-13 | The Board Of Trustees Of The University Of Illinois | Label-free functional nucleic acid sensors for detecting target agents |
CN103305612B (zh) * | 2013-06-04 | 2015-01-07 | 西安交通大学 | 一种基于恒温级联核酸扩增的铅离子检测试剂盒及其检测方法 |
US11142544B2 (en) * | 2014-04-28 | 2021-10-12 | Juewen Liu | Phosphorothioate DNAzyme complexes and use thereof |
CN107217100B (zh) * | 2017-06-29 | 2020-11-24 | 中国农业大学 | 基于dna酶嵌合引物的核酸筛查生物传感器 |
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Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1850981A (zh) * | 2006-03-10 | 2006-10-25 | 杭州优思达生物技术有限公司 | 切口酶扩增靶核酸序列的方法及用于扩增靶核酸序列的试剂盒及其应用 |
CN105136875A (zh) * | 2015-07-31 | 2015-12-09 | 江苏巨珩新材料科技有限公司 | 利用超级三明治核酸探针纳米孔道传感器高灵敏高特异性检测Zn2+的方法 |
CN106337082A (zh) * | 2016-04-28 | 2017-01-18 | 湖南大学 | 基于酶法生成随机g四链体的细胞凋亡免标记检测方法 |
CN106520927A (zh) * | 2016-09-23 | 2017-03-22 | 广东省生态环境技术研究所 | 一种核酸检测方法及试剂盒 |
CN107012208A (zh) * | 2017-03-08 | 2017-08-04 | 广东省生态环境技术研究所 | 一种免标记铅离子可视化检测方法及检测试剂盒 |
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