CN107954764A - A kind of preparation method of cucumber organic fertilizer - Google Patents
A kind of preparation method of cucumber organic fertilizer Download PDFInfo
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- CN107954764A CN107954764A CN201711428546.7A CN201711428546A CN107954764A CN 107954764 A CN107954764 A CN 107954764A CN 201711428546 A CN201711428546 A CN 201711428546A CN 107954764 A CN107954764 A CN 107954764A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/60—Biocides or preservatives, e.g. disinfectants, pesticides or herbicides; Pest repellants or attractants
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Pest Control & Pesticides (AREA)
- Biochemistry (AREA)
- Plant Pathology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Soil Sciences (AREA)
- Biotechnology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Fertilizers (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a kind of preparation method of cucumber organic fertilizer, comprise the following steps：Preparing microorganism nutrient powder；Stalk, peanut shell, bagasse, wheat bran and animal excreta are built into heap fermentation；Then mixed with chitosan, expanded vermiculite, fertile soil, hydroscopic high-molecular resin, potassium carbonate, Boratex, ammonium phosphate, N P K ternary compound fertilizer, microbial nutrition powder is mixed after dry.Cucumber organic fertilizer retain water and nutrients are prepared in the method according to the invention, can promote cucumber growth, improve cucumber yield, reduce soil-borne disease of cucumber incidence its increase gas permeability of soil.
Description
Technical field
The present invention relates to a kind of preparation method of cucumber organic fertilizer.
Background technology
Cucumber is the fruits and vegetables of people's often feeding, and cucumber needs substantial amounts of moisture and fertility during plantation, no
When fertilising can cause suddenly it is hardened, pest and disease damage is multiple.Traditional farm manure can overcome soil hardening, chemical residual etc. to ask
Topic, but it contains substantial amounts of harmful microorganism, blindly fertilising can cause the propagation of pathogenic microorganism and parasitic ovum.It is commercially available
Mostly in chemical fertilizer is that water-retaining property is poor containing a variety of inorganic fertilizer, and long-time service can cause the hardened of soil, in addition, can also shadow
Ring the mouthfeel of cucumber.
The content of the invention
To solve above-mentioned technical problem.The present invention provides a kind of preparation method of cucumber organic fertilizer.The cucumber is organic
Fertile retain water and nutrients, can promote cucumber growth, raising cucumber yield, reduce the incidence of soil-borne disease of cucumber and increase soil and is saturating
Gas.
The technical solution that the present invention takes is：
A kind of preparation method of cucumber organic fertilizer, the preparation method comprise the following steps：
1) prepare containing bacillus thuringiensis, bacillus subtilis, the light microbial nutrition powder for intending purple Penicillium notatum；
2) stalk, peanut shell, bagasse are crushed, is uniformly mixed with wheat bran, animal wastes, then spray EM bacterium solutions, protected
Humidity is held to carry out building heap fermentation 5~7 days for 45~60%；
3) by chitosan, expanded vermiculite, fertile soil, hydroscopic high-molecular resin, potassium carbonate, Boratex, ammonium phosphate, N-P-K
Ternary compound fertilizer is added in step 2) and is uniformly mixed；
4) semi-finished product obtained by step 3) are dried to moisture below 7% in 45~60 DEG C, is then obtained with step 1)
To microbial nutrition powder pack after mixing, you can obtain the cucumber organic fertilizer.
The parts by weight of each raw material are as follows：3~12 parts of chitosan, 5~16 parts of expanded vermiculite, 15~30 parts of animal wastes, straw
20~50 parts of stalk, 10~30 parts of peanut shell, 10~25 parts of bagasse, 5~15 parts of wheat bran, 20~40 parts of fertile soil, EM bacterium solutions 1~
6 parts, 4~12 parts of hydroscopic high-molecular resin, 5~15 parts of microbial nutrition powder, 2~5 parts of potassium carbonate, 4~9 parts of Boratex, phosphoric acid
Ammonium 1~6
Further, it is preferable to the parts by weight for each raw material are as follows：5~9 parts of chitosan, 8~14 parts of expanded vermiculite, animal excreta
Just 18~27 parts, 29~41 parts of stalk, 15~25 parts of peanut shell, 14~22 parts of bagasse, 7~11 parts of wheat bran, fertile soil 27~
30 parts, 3~5 parts of EM bacterium solutions, 7~10 parts of hydroscopic high-molecular resin, 7~12 parts of microbial nutrition powder, 3~4 parts of potassium carbonate, boric acid
4~7 parts of sodium, 2~4 parts of ammonium phosphate, 4~6 parts of N-P-K ternary compound fertilizer.
Further, the parts by weight of each raw material are as follows：6 parts of chitosan, 12 parts of expanded vermiculite, 22 parts of animal wastes, straw
35 parts of stalk, 22 parts of peanut shell, 17 parts of bagasse, 8 parts of wheat bran, 28 parts of fertile soil, 5 parts of EM bacterium solutions, 8 parts of hydroscopic high-molecular resin,
10 parts of microbial nutrition powder, 3 parts of potassium carbonate, 6 parts of Boratex, 3 parts of ammonium phosphate, 5 parts of N-P-K ternary compound fertilizer.
Further, the step 1) comprises the following steps：
A. first by bacillus thuringiensis, bacillus subtilis, it is light intend purple Penicillium notatum respectively through fluid nutrient medium culture extremely
Target viable count is 2~1,000,000,000/mL；
B. each strain liquid for and then again obtaining step A is according to weight ratio 1：1~2：0.5~1.5 mixing, with 15~20%
Inoculum concentration by strain mixed liquor be inoculated into intersect antagonism fluid nutrient medium in cultivate 6~8h；
C. the step B bacterium solutions cultivated are inoculated into powder culture medium, adjustment target viable count is 5~1,000,000,000/g.
The component for intersecting antagonism fluid nutrient medium in the step A in fluid nutrient medium and the step B is：Every liter of training
Support in base and contain following material：20~40g of peptone, 30~50g of agar, 35~45g of molasses, 10~20g of glucose, di(2-ethylhexyl)phosphate
Hydrogen 2~4g of potassium, 0.5~1.5g of sodium carbonate, remaining is distilled water, pH 7.0.
The component of powder culture medium is in the step C：30~50 parts of corn flour, 50~70 parts of wheat bran, glucose 15~
20 parts.
The preparation method of the expanded vermiculite is：By vermiculite through 300~400 DEG C of 1~2h of roasting, you can obtain the expansion
Vermiculite.
Further, in the step (2), when building heap fermentation, treat that fermentation temperature rises to 45~55 DEG C, turning once, after
Turning is once when 24 is small in face.
In the preparation method of cucumber fertilizer special for organic provided by the invention, chitosan can improve the structure of soil, can be
Beneficial microorganism growth in soil provides energy, promotes beneficial microorganism amount reproduction, the amount reproduction of microorganism can
Promote the formation of soil aggregate structure, improve the physicochemical property of soil, strengthen gas permeability and fertilizer-preserving ability, in addition chitosan molecule
Contain amino in structure, chelation can be produced with minor metallic element, make the micro- available nutrients in fertilizer
Increase, improves the fertilizer efficiency of organic fertilizer.
Volume, which is expandable to 15~20 times, after vermiculite is fired becomes expanded vermiculite, its surface area is significantly expanded, layer and layer
Between separate thorough, interlayer hole increases, and can dramatically increase the gas permeability of soil, increases the retain water and nutrients ability of soil.
Animal wastes, stalk, peanut shell, these materials of bagasse through EM bacterium solutions fermentation after can produce substantial amounts of organic matter,
Humic acid, amino acid, mineral matter, trace element etc., comprehensive nutrition can be provided for the growth of cucumber by coordinating with other raw materials.
Hydroscopic high-molecular resin has stronger water absorbing capacity, is expanded rapidly after water suction, then slow release, prevents moisture
Rapid evaporation, and the growth of cucumber needs substantial amounts of moisture, and the addition of hydroscopic high-molecular resin can significantly reduce pouring for cucumber
Waterside number, saves the energy, and has effects that fertilizer conservation.
Microbial nutrition powder is through training respectively by bacillus thuringiensis, bacillus subtilis and light purple Penicillium notatum of intending
After supporting, intersecting antagonism culture again, the nutritional powder prepared, for three kinds of strains after intersecting and cultivating, each mutual antagonism of strain is competing
Strive growth, form beneficial microbe colony, in this flora, bacillus subtilis can inhibit and kill harmful bacteria in soil,
Pathogen；Bacillus thuringiensis suppresses and kills the pest in soil and worm's ovum；Light purple Penicillium notatum of intending can effectively reduce nematode
The harm of disease, three kinds of strains act synergistically jointly, can effectively prevent the generation of Cucumber Pests And Diseases, each strain of the above is intended to be limited to
Within the scope of specifying, not so easily because of its flora imbalance, the effect of obtained organic fertilizer, can substantially reduce.
Each raw material collective effect above so that cucumber fertilizer special for organic provided by the invention, has the following advantages：
1. nutrition is comprehensive, in this organic fertilizer in addition to nitrogen, phosphorus, potassium necessary to containing cucumber growth, also it is especially added with
Animal wastes, stalk, the fermentate of peanut shell and bagasse, it gives cucumber to provide rich in nutritional ingredients such as organic matter, humic acid
Comprehensive nutrition supply；
2. suppressing the occurrence probability of pest and disease damage, the microbial nutrition powder in fertilizer is rapid raw in the soil after fertilising
Long breeding, produces a large amount of beneficial bacteriums, and protective barrier is formed around cucumber root, suppresses harmful bacteria and disease, farthest
Reduce the generation of soil-borne disease；
3. improving soil, retain water and nutrients, increase cucumber yield.Made by the fixed nitrogen of beneficial microbe colony, phosphorus decomposing, potassium decomposing
With with reference to expanded vermiculite and loose structure, loose activating soil, mitigates soil hardening, Balancing；In addition macromolecule is combined to inhale
Water-resin is beneficial to storing water and preserving fertility, improves fertilizer efficiency.
Embodiment
Embodiment 1
A kind of preparation method of cucumber organic fertilizer, comprises the following steps：
1) prepare containing bacillus thuringiensis, bacillus subtilis, the light microbial nutrition powder for intending purple Penicillium notatum；
2) stalk, peanut shell, bagasse are crushed, is uniformly mixed with wheat bran, animal wastes, then spray EM bacterium solutions, protected
Humidity is held to carry out building heap fermentation 6 days for 55%；When building heap fermentation, treat that fermentation temperature rises to 50 DEG C, turning once, behind every 24
Hour turning is once；
3) by chitosan, expanded vermiculite, fertile soil, hydroscopic high-molecular resin, potassium carbonate, Boratex, ammonium phosphate, N-P-K
Ternary compound fertilizer is added in step 2) and is uniformly mixed；
4) semi-finished product obtained by step 3) are dried to moisture below 7% in 50 DEG C, is then obtained with step 1)
Microbial nutrition powder is packed after mixing, you can obtains the cucumber organic fertilizer.
The parts by weight of each raw material are as follows in above step：6 parts of chitosan, 12 parts of expanded vermiculite, 22 parts of animal wastes, stalk
35 parts, it is 22 parts of peanut shell, 17 parts of bagasse, 8 parts of wheat bran, 28 parts of fertile soil, 5 parts of EM bacterium solutions, 8 parts of hydroscopic high-molecular resin, micro-
10 parts of biological nutrition powder, 3 parts of potassium carbonate, 6 parts of Boratex, 3 parts of ammonium phosphate, 5 parts of N-P-K ternary compound fertilizer.
The step 1) specifically includes following steps：
A. first by bacillus thuringiensis, bacillus subtilis, it is light intend purple Penicillium notatum respectively through fluid nutrient medium culture extremely
Target viable count is 500,000,000/mL；
B. each strain liquid for and then again obtaining step A is according to weight ratio 1：1：1.5 mixing, with 15% inoculum concentration by bacterium
Kind mixed liquor, which is inoculated into intersect in antagonism fluid nutrient medium, cultivates 8h；
C. the step B bacterium solutions cultivated are inoculated into powder culture medium, adjustment target viable count is 500,000,000/g.
The component for intersecting antagonism fluid nutrient medium in the step A in fluid nutrient medium and the step B is：Every liter of training
Support in base and contain following material：Peptone 20g, agar 50g, molasses 35g, glucose 10g, potassium dihydrogen phosphate 2g, sodium carbonate
1.5g, remaining is distilled water, pH 7.0.
The component of powder culture medium is in the step C：50 parts of corn flour, 50 parts of wheat bran, 15 parts of glucose.
Embodiment 2
A kind of preparation method of cucumber organic fertilizer, comprises the following steps：
1) prepare containing bacillus thuringiensis, bacillus subtilis, the light microbial nutrition powder for intending purple Penicillium notatum；
2) stalk, peanut shell, bagasse are crushed, is uniformly mixed with wheat bran, animal wastes, then spray EM bacterium solutions, protected
Humidity is held to carry out building heap fermentation 6 days for 50%；Treat that fermentation temperature rises to 50 DEG C, turning once, behind the turning one when 24 is small
It is secondary；
3) by chitosan, expanded vermiculite, fertile soil, hydroscopic high-molecular resin, potassium carbonate, Boratex, ammonium phosphate, N-P-K
Ternary compound fertilizer is added in step 2) and is uniformly mixed；
4) semi-finished product obtained by step 3) are dried to moisture below 7% in 50 DEG C, is then obtained with step 1)
Microbial nutrition powder is packed after mixing, you can obtains the cucumber organic fertilizer.
The parts by weight of each raw material are as follows in above step：5 parts of chitosan, 8 parts of expanded vermiculite, 18 parts of animal wastes, stalk
29 parts, it is 15 parts of peanut shell, 14 parts of bagasse, 7 parts of wheat bran, 27 parts of fertile soil, 3 parts of EM bacterium solutions, 7 parts of hydroscopic high-molecular resin, micro-
7 parts of biological nutrition powder, 3 parts of potassium carbonate, 4 parts of Boratex, 2 parts of ammonium phosphate, 4 parts of N-P-K ternary compound fertilizer.
The step 1) specifically includes following steps：
A. first by bacillus thuringiensis, bacillus subtilis, it is light intend purple Penicillium notatum respectively through fluid nutrient medium culture extremely
Target viable count is 300,000,000/mL；
B. each strain liquid for and then again obtaining step A is according to weight ratio 1：2：0.5 mixing, with 20% inoculum concentration by bacterium
Kind mixed liquor, which is inoculated into intersect in antagonism fluid nutrient medium, cultivates 8h；
C. the step B bacterium solutions cultivated are inoculated into powder culture medium, adjustment target viable count is 800,000,000/g.
The component for intersecting antagonism fluid nutrient medium in the step A in fluid nutrient medium and the step B is：Every liter of training
Support in base and contain following material：Peptone 40g, agar 30g, molasses 40g, glucose 20g, potassium dihydrogen phosphate 3g, sodium carbonate
0.5g, remaining is distilled water, pH 7.0.
The component of powder culture medium is in the step C：30 parts of corn flour, 70 parts of wheat bran, 15 parts of glucose.
Embodiment 3
A kind of preparation method of cucumber organic fertilizer, comprises the following steps：
1) prepare containing bacillus thuringiensis, bacillus subtilis, the light microbial nutrition powder for intending purple Penicillium notatum；
2) stalk, peanut shell, bagasse are crushed, is uniformly mixed with wheat bran, animal wastes, then spray EM bacterium solutions, protected
Humidity is held to carry out building heap fermentation 5 days for 60%；Treat that fermentation temperature rises to 55 DEG C, turning once, behind the turning one when 24 is small
It is secondary；
3) by chitosan, expanded vermiculite, fertile soil, hydroscopic high-molecular resin, potassium carbonate, Boratex, ammonium phosphate, N-P-K
Ternary compound fertilizer is added in step 1) and is uniformly mixed；
4) semi-finished product obtained by step 3) are dried to moisture below 7% in 60 DEG C, is then obtained with step 1)
Microbial nutrition powder is packed after mixing, you can obtains the cucumber organic fertilizer.
The parts by weight of each raw material are as follows in above step：9 parts of chitosan, 14 parts of expanded vermiculite, 27 parts of animal wastes, stalk
41 parts, 25 parts of peanut shell, 22 parts of bagasse, 11 parts of wheat bran, 30 parts of fertile soil, 5 parts of EM bacterium solutions, 10 parts of hydroscopic high-molecular resin,
12 parts of microbial nutrition powder, 4 parts of potassium carbonate, 7 parts of Boratex, 4 parts of ammonium phosphate, 6 parts of N-P-K ternary compound fertilizer.
The step 1) specifically includes following steps：
A. first by bacillus thuringiensis, bacillus subtilis, it is light intend purple Penicillium notatum respectively through fluid nutrient medium culture extremely
Target viable count is 1,000,000,000/mL；
B. each strain liquid for and then again obtaining step A is according to weight ratio 1：1.5：1.0 mixing, will with 20% inoculum concentration
Strain mixed liquor, which is inoculated into intersect in antagonism fluid nutrient medium, cultivates 8h；
C. the step B bacterium solutions cultivated are inoculated into powder culture medium, adjustment target viable count is 1,000,000,000/g.
The component for intersecting antagonism fluid nutrient medium in the step A in fluid nutrient medium and the step B is：Every liter of training
Support in base and contain following material：Peptone 30g, agar 40g, molasses 45g, glucose 20g, potassium dihydrogen phosphate 3g, sodium carbonate
1.0g, remaining is distilled water, pH 7.0.
The component of powder culture medium is in the step C：40 parts of corn flour, 60 parts of wheat bran, 15 parts of glucose.
The organic fertilizer that various embodiments of the present invention obtain as base manure when carrying out green cucumber, it is organic compared with the routine of in the market
Fertilizer, cucumber yield can improve 10~15%, and pest and disease damage incidence is extremely low.
The above-mentioned detailed description carried out with reference to embodiment to the preparation method of cucumber organic fertilizer a kind of, be it is illustrative without
It is limited, several embodiments can be included according to limited scope, therefore in the case where not departing from present general inventive concept
Change and modifications, should belong within protection scope of the present invention.
Claims (9)
1. a kind of preparation method of cucumber organic fertilizer, it is characterised in that the preparation method comprises the following steps：
1) prepare containing bacillus thuringiensis, bacillus subtilis, the light microbial nutrition powder for intending purple Penicillium notatum；
2) stalk, peanut shell, bagasse are crushed, is uniformly mixed with wheat bran, animal wastes, then spray EM bacterium solutions, kept wet
Spend and carry out building heap fermentation 5~7 days for 45~60%；
3) by chitosan, expanded vermiculite, fertile soil, hydroscopic high-molecular resin, potassium carbonate, Boratex, ammonium phosphate, N-P-K ternarys
Composite fertilizer is added in step 2) and is uniformly mixed；
4) semi-finished product obtained by step 3) are dried to moisture below 7% in 45~60 DEG C, is then obtained with step 1)
Microbial nutrition powder is packed after mixing, you can obtains the cucumber organic fertilizer.
2. preparation method according to claim 1, it is characterised in that the parts by weight of each raw material are as follows：Chitosan 3~12
Part, 5~16 parts of expanded vermiculite, 15~30 parts of animal wastes, 20~50 parts of stalk, 10~30 parts of peanut shell, bagasse 10~25
Part, 5~15 parts of wheat bran, 20~40 parts of fertile soil, 1~6 part of EM bacterium solutions, 4~12 parts of hydroscopic high-molecular resin, microbial nutrition powder
5~15 parts, 2~5 parts of potassium carbonate, 4~9 parts of Boratex, 1~6 part of ammonium phosphate, 3~8 parts of N-P-K ternary compound fertilizer.
3. preparation method according to claim 1, it is characterised in that the parts by weight of each raw material are as follows：5~9 parts of chitosan,
8~14 parts of expanded vermiculite, 18~27 parts of animal wastes, 29~41 parts of stalk, 15~25 parts of peanut shell, 14~22 parts of bagasse,
7~11 parts of wheat bran, 27~30 parts of fertile soil, 3~5 parts of EM bacterium solutions, 7~10 parts of hydroscopic high-molecular resin, microbial nutrition powder 7~
12 parts, 3~4 parts of potassium carbonate, 4~7 parts of Boratex, 2~4 parts of ammonium phosphate, 4~6 parts of N-P-K ternary compound fertilizer.
4. preparation method according to claim 1, it is characterised in that the parts by weight of each raw material are as follows：It is 6 parts of chitosan, swollen
Swollen 12 parts of vermiculite, 22 parts of animal wastes, 35 parts of stalk, 22 parts of peanut shell, 17 parts of bagasse, 8 parts of wheat bran, 28 parts of fertile soil, EM
5 parts of bacterium solution, 8 parts of hydroscopic high-molecular resin, 10 parts of microbial nutrition powder, 3 parts of potassium carbonate, 6 parts of Boratex, 3 parts of ammonium phosphate, N-P-
5 parts of K ternary compound fertilizer.
5. preparation method according to claim 1, it is characterised in that the step 1) comprises the following steps：
A. first by bacillus thuringiensis, bacillus subtilis, light purple Penicillium notatum of intending respectively through fluid nutrient medium culture to target
Viable count is 2~1,000,000,000/mL；
B. each strain liquid for and then again obtaining step A is according to weight ratio 1：1~2：0.5~1.5 mixing, connects with 15~20%
Strain mixed liquor is inoculated into intersect by kind amount cultivates 6~8h in antagonism fluid nutrient medium；
C. the step B bacterium solutions cultivated are inoculated into powder culture medium, adjustment target viable count is 5~1,000,000,000/g.
6. preparation method according to claim 4, it is characterised in that fluid nutrient medium and the step B in the step A
It is middle intersect antagonism fluid nutrient medium component be：Contain following material in every liter of culture medium：20~40g of peptone, agar 30
~50g, 35~45g of molasses, 10~20g of glucose, 2~4g of potassium dihydrogen phosphate, 0.5~1.5g of sodium carbonate, remaining is distilled water,
PH is 7.0.
7. preparation method according to claim 4, it is characterised in that the component of powder culture medium is in the step C：It is beautiful
30~50 parts of rice flour, 50~70 parts of wheat bran, 15~20 parts of glucose.
8. preparation method according to claim 1, it is characterised in that the preparation method of the expanded vermiculite is：By vermiculite
Through 300~400 DEG C of 1~2h of roasting, you can obtain the expanded vermiculite.
9. preparation method according to claim 1, it is characterised in that in the step (2), when building heap fermentation, wait to ferment
Temperature rises to 45~55 DEG C, turning once, behind turning is once when 24 is small.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113135777A (en) * | 2021-04-22 | 2021-07-20 | 广西壮族自治区农业科学院 | On-site harmless treatment method for fruit and vegetable wastes |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20150307408A1 (en) * | 2012-03-27 | 2015-10-29 | Agrinos AS | Microbial composition comprising liquid fertilizer and processes for agricultural use |
| CN106747933A (en) * | 2016-11-22 | 2017-05-31 | 临沂市农业科学院 | A kind of Biological organic fertilizer special for cucumber and preparation method thereof |
| CN107365207A (en) * | 2017-09-07 | 2017-11-21 | 芜湖皖江知识产权运营中心有限公司 | A kind of solid-state biological organic fertilizer and preparation method thereof |
-
2017
- 2017-12-26 CN CN201711428546.7A patent/CN107954764A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20150307408A1 (en) * | 2012-03-27 | 2015-10-29 | Agrinos AS | Microbial composition comprising liquid fertilizer and processes for agricultural use |
| CN106747933A (en) * | 2016-11-22 | 2017-05-31 | 临沂市农业科学院 | A kind of Biological organic fertilizer special for cucumber and preparation method thereof |
| CN107365207A (en) * | 2017-09-07 | 2017-11-21 | 芜湖皖江知识产权运营中心有限公司 | A kind of solid-state biological organic fertilizer and preparation method thereof |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113135777A (en) * | 2021-04-22 | 2021-07-20 | 广西壮族自治区农业科学院 | On-site harmless treatment method for fruit and vegetable wastes |
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