CN107937454A - 一种固定化酶催化剂生物合成d‑塔格糖的方法 - Google Patents
一种固定化酶催化剂生物合成d‑塔格糖的方法 Download PDFInfo
- Publication number
- CN107937454A CN107937454A CN201711395868.6A CN201711395868A CN107937454A CN 107937454 A CN107937454 A CN 107937454A CN 201711395868 A CN201711395868 A CN 201711395868A CN 107937454 A CN107937454 A CN 107937454A
- Authority
- CN
- China
- Prior art keywords
- immobilized enzyme
- catalysis agent
- enzyme catalysis
- bacillus subtilis
- biosynthesis
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 108010093096 Immobilized Enzymes Proteins 0.000 title claims abstract description 52
- 238000006555 catalytic reaction Methods 0.000 title claims abstract description 52
- 238000000034 method Methods 0.000 title claims abstract description 36
- 230000015572 biosynthetic process Effects 0.000 title claims abstract description 23
- 150000003474 tagatoses Chemical class 0.000 title abstract 8
- 238000006243 chemical reaction Methods 0.000 claims abstract description 116
- 102000004190 Enzymes Human genes 0.000 claims abstract description 82
- 108090000790 Enzymes Proteins 0.000 claims abstract description 82
- 108010018080 L-arabinose isomerase Proteins 0.000 claims abstract description 57
- 244000063299 Bacillus subtilis Species 0.000 claims abstract description 45
- 235000014469 Bacillus subtilis Nutrition 0.000 claims abstract description 45
- 241000726221 Gemma Species 0.000 claims abstract description 36
- 230000000694 effects Effects 0.000 claims abstract description 22
- 230000007704 transition Effects 0.000 claims abstract description 3
- 229940088598 enzyme Drugs 0.000 claims description 81
- LKDRXBCSQODPBY-OEXCPVAWSA-N D-tagatose Chemical compound OCC1(O)OC[C@@H](O)[C@H](O)[C@@H]1O LKDRXBCSQODPBY-OEXCPVAWSA-N 0.000 claims description 72
- 239000003795 chemical substances by application Substances 0.000 claims description 46
- 230000009466 transformation Effects 0.000 claims description 12
- 239000007788 liquid Substances 0.000 claims description 10
- 239000000872 buffer Substances 0.000 claims description 9
- 238000005119 centrifugation Methods 0.000 claims description 9
- 229910021380 Manganese Chloride Inorganic materials 0.000 claims description 8
- 239000011565 manganese chloride Substances 0.000 claims description 8
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 6
- 239000000203 mixture Substances 0.000 claims description 6
- 239000008363 phosphate buffer Substances 0.000 claims description 5
- 238000012545 processing Methods 0.000 claims description 5
- 239000013049 sediment Substances 0.000 claims description 4
- 102000016943 Muramidase Human genes 0.000 claims description 3
- 108010014251 Muramidase Proteins 0.000 claims description 3
- 108010062010 N-Acetylmuramoyl-L-alanine Amidase Proteins 0.000 claims description 3
- 229960000274 lysozyme Drugs 0.000 claims description 3
- 239000004325 lysozyme Substances 0.000 claims description 3
- 235000010335 lysozyme Nutrition 0.000 claims description 3
- 238000002360 preparation method Methods 0.000 claims description 3
- 239000011780 sodium chloride Substances 0.000 claims description 3
- 238000004519 manufacturing process Methods 0.000 abstract description 21
- 238000005516 engineering process Methods 0.000 abstract description 7
- 238000003786 synthesis reaction Methods 0.000 abstract description 4
- 235000021393 food security Nutrition 0.000 abstract description 3
- 230000008569 process Effects 0.000 abstract description 3
- 108090000623 proteins and genes Proteins 0.000 description 23
- 239000000758 substrate Substances 0.000 description 21
- 238000004064 recycling Methods 0.000 description 20
- 239000000243 solution Substances 0.000 description 19
- 239000013612 plasmid Substances 0.000 description 12
- 210000004027 cell Anatomy 0.000 description 11
- 241000276408 Bacillus subtilis subsp. subtilis str. 168 Species 0.000 description 6
- 241000186840 Lactobacillus fermentum Species 0.000 description 6
- 230000008859 change Effects 0.000 description 6
- BJHIKXHVCXFQLS-PQLUHFTBSA-N keto-D-tagatose Chemical compound OC[C@@H](O)[C@H](O)[C@H](O)C(=O)CO BJHIKXHVCXFQLS-PQLUHFTBSA-N 0.000 description 6
- 229940012969 lactobacillus fermentum Drugs 0.000 description 6
- 239000000047 product Substances 0.000 description 6
- 240000001929 Lactobacillus brevis Species 0.000 description 5
- 235000013957 Lactobacillus brevis Nutrition 0.000 description 5
- 101150035354 araA gene Proteins 0.000 description 5
- 239000012530 fluid Substances 0.000 description 5
- 238000010998 test method Methods 0.000 description 5
- 241000193830 Bacillus <bacterium> Species 0.000 description 4
- 240000006024 Lactobacillus plantarum Species 0.000 description 4
- 235000013965 Lactobacillus plantarum Nutrition 0.000 description 4
- 238000012408 PCR amplification Methods 0.000 description 4
- 244000057717 Streptococcus lactis Species 0.000 description 4
- 235000014897 Streptococcus lactis Nutrition 0.000 description 4
- 239000005862 Whey Substances 0.000 description 4
- 102000007544 Whey Proteins Human genes 0.000 description 4
- 108010046377 Whey Proteins Proteins 0.000 description 4
- 230000001580 bacterial effect Effects 0.000 description 4
- 239000007853 buffer solution Substances 0.000 description 4
- 238000009413 insulation Methods 0.000 description 4
- 230000010354 integration Effects 0.000 description 4
- 229940072205 lactobacillus plantarum Drugs 0.000 description 4
- LWIHDJKSTIGBAC-UHFFFAOYSA-K potassium phosphate Substances [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 description 4
- 229930006000 Sucrose Natural products 0.000 description 3
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 3
- 230000003115 biocidal effect Effects 0.000 description 3
- 235000013305 food Nutrition 0.000 description 3
- 239000012634 fragment Substances 0.000 description 3
- 230000004927 fusion Effects 0.000 description 3
- 238000012215 gene cloning Methods 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 238000012163 sequencing technique Methods 0.000 description 3
- 239000005720 sucrose Substances 0.000 description 3
- 239000013598 vector Substances 0.000 description 3
- IXPNQXFRVYWDDI-UHFFFAOYSA-N 1-methyl-2,4-dioxo-1,3-diazinane-5-carboximidamide Chemical compound CN1CC(C(N)=N)C(=O)NC1=O IXPNQXFRVYWDDI-UHFFFAOYSA-N 0.000 description 2
- 101000911390 Homo sapiens Coagulation factor VIII Proteins 0.000 description 2
- 240000002648 Lactobacillus brevis ATCC 367 Species 0.000 description 2
- 235000007048 Lactobacillus brevis ATCC 367 Nutrition 0.000 description 2
- OIRDTQYFTABQOQ-UHTZMRCNSA-N Vidarabine Chemical group C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](CO)[C@@H](O)[C@@H]1O OIRDTQYFTABQOQ-UHTZMRCNSA-N 0.000 description 2
- 239000000654 additive Substances 0.000 description 2
- 230000000996 additive effect Effects 0.000 description 2
- 238000000246 agarose gel electrophoresis Methods 0.000 description 2
- 238000005267 amalgamation Methods 0.000 description 2
- 238000000137 annealing Methods 0.000 description 2
- 239000003054 catalyst Substances 0.000 description 2
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 2
- 230000004087 circulation Effects 0.000 description 2
- 238000010367 cloning Methods 0.000 description 2
- 230000000295 complement effect Effects 0.000 description 2
- 238000010276 construction Methods 0.000 description 2
- 238000013461 design Methods 0.000 description 2
- 230000029087 digestion Effects 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 102000057593 human F8 Human genes 0.000 description 2
- 238000005457 optimization Methods 0.000 description 2
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 description 2
- 235000011009 potassium phosphates Nutrition 0.000 description 2
- 235000013406 prebiotics Nutrition 0.000 description 2
- 229940047431 recombinate Drugs 0.000 description 2
- 230000004044 response Effects 0.000 description 2
- 230000000284 resting effect Effects 0.000 description 2
- 235000010413 sodium alginate Nutrition 0.000 description 2
- 239000000661 sodium alginate Substances 0.000 description 2
- 229940005550 sodium alginate Drugs 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- AEMOLEFTQBMNLQ-UHFFFAOYSA-N 3,4,5,6-tetrahydroxyoxane-2-carboxylic acid Chemical compound OC1OC(C(O)=O)C(O)C(O)C1O AEMOLEFTQBMNLQ-UHFFFAOYSA-N 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 206010009944 Colon cancer Diseases 0.000 description 1
- 241000672609 Escherichia coli BL21 Species 0.000 description 1
- 241000194036 Lactococcus Species 0.000 description 1
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 1
- 208000002720 Malnutrition Diseases 0.000 description 1
- GLFNIEUTAYBVOC-UHFFFAOYSA-L Manganese chloride Chemical compound Cl[Mn]Cl GLFNIEUTAYBVOC-UHFFFAOYSA-L 0.000 description 1
- 206010054949 Metaplasia Diseases 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- 239000004365 Protease Substances 0.000 description 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 1
- 208000007502 anemia Diseases 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- OIRDTQYFTABQOQ-UHFFFAOYSA-N ara-adenosine Natural products Nc1ncnc2n(cnc12)C1OC(CO)C(O)C1O OIRDTQYFTABQOQ-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 235000013361 beverage Nutrition 0.000 description 1
- 239000006227 byproduct Substances 0.000 description 1
- 230000003197 catalytic effect Effects 0.000 description 1
- 230000033077 cellular process Effects 0.000 description 1
- 235000015218 chewing gum Nutrition 0.000 description 1
- 229940112822 chewing gum Drugs 0.000 description 1
- 235000012000 cholesterol Nutrition 0.000 description 1
- 208000029742 colonic neoplasm Diseases 0.000 description 1
- 235000013365 dairy product Nutrition 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 206010012601 diabetes mellitus Diseases 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 230000006353 environmental stress Effects 0.000 description 1
- 239000013604 expression vector Substances 0.000 description 1
- 235000003599 food sweetener Nutrition 0.000 description 1
- 235000015203 fruit juice Nutrition 0.000 description 1
- 235000021474 generally recognized As safe (food) Nutrition 0.000 description 1
- 235000021473 generally recognized as safe (food ingredients) Nutrition 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 238000010353 genetic engineering Methods 0.000 description 1
- 230000006801 homologous recombination Effects 0.000 description 1
- 238000002744 homologous recombination Methods 0.000 description 1
- 210000001822 immobilized cell Anatomy 0.000 description 1
- 230000008676 import Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 229940039696 lactobacillus Drugs 0.000 description 1
- 239000008101 lactose Substances 0.000 description 1
- 230000015689 metaplastic ossification Effects 0.000 description 1
- 235000018343 nutrient deficiency Nutrition 0.000 description 1
- 230000001151 other effect Effects 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 230000003334 potential effect Effects 0.000 description 1
- 239000006041 probiotic Substances 0.000 description 1
- 235000018291 probiotics Nutrition 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 230000006798 recombination Effects 0.000 description 1
- 238000005215 recombination Methods 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000003765 sweetening agent Substances 0.000 description 1
- 238000012549 training Methods 0.000 description 1
- 208000001072 type 2 diabetes mellitus Diseases 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/02—Monosaccharides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/16—Enzymes or microbial cells immobilised on or in a biological cell
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/90—Isomerases (5.)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y503/00—Intramolecular oxidoreductases (5.3)
- C12Y503/01—Intramolecular oxidoreductases (5.3) interconverting aldoses and ketoses (5.3.1)
- C12Y503/01003—Arabinose isomerase (5.3.1.3)
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
Abstract
Description
Claims (9)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201711395868.6A CN107937454B (zh) | 2017-12-21 | 2017-12-21 | 一种固定化酶催化剂生物合成d-塔格糖的方法 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201711395868.6A CN107937454B (zh) | 2017-12-21 | 2017-12-21 | 一种固定化酶催化剂生物合成d-塔格糖的方法 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN107937454A true CN107937454A (zh) | 2018-04-20 |
CN107937454B CN107937454B (zh) | 2020-05-01 |
Family
ID=61941042
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201711395868.6A Active CN107937454B (zh) | 2017-12-21 | 2017-12-21 | 一种固定化酶催化剂生物合成d-塔格糖的方法 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN107937454B (zh) |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN112852702A (zh) * | 2021-03-16 | 2021-05-28 | 江南大学 | 一种重组枯草芽孢杆菌催化高浓度乳糖合成塔格糖的方法 |
CN115161310A (zh) * | 2022-08-19 | 2022-10-11 | 大连工业大学 | 一种d-半乳糖异构催化剂及其制备和应用 |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN112708616B (zh) | 2021-03-29 | 2021-08-20 | 中国科学院天津工业生物技术研究所 | 一种固定化多酶体系生产塔格糖的方法 |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101475954A (zh) * | 2008-12-05 | 2009-07-08 | 江苏大学 | 表面展示具有催化活性的脂肪酶的重组芽孢的制备方法 |
CN102888389A (zh) * | 2012-08-31 | 2013-01-23 | 江苏梁丰食品集团有限公司 | 一种高效诱导l-阿拉伯糖异构酶的方法 |
CN104673817A (zh) * | 2015-02-13 | 2015-06-03 | 江苏大学 | 一种脂肪酶的原核表达及应用和固定化方法 |
CN105132450A (zh) * | 2015-09-09 | 2015-12-09 | 齐鲁工业大学 | 枯草芽孢杆菌芽孢衣壳蛋白Cot表面展示海藻糖合酶的方法 |
-
2017
- 2017-12-21 CN CN201711395868.6A patent/CN107937454B/zh active Active
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101475954A (zh) * | 2008-12-05 | 2009-07-08 | 江苏大学 | 表面展示具有催化活性的脂肪酶的重组芽孢的制备方法 |
CN102888389A (zh) * | 2012-08-31 | 2013-01-23 | 江苏梁丰食品集团有限公司 | 一种高效诱导l-阿拉伯糖异构酶的方法 |
CN104673817A (zh) * | 2015-02-13 | 2015-06-03 | 江苏大学 | 一种脂肪酶的原核表达及应用和固定化方法 |
CN105132450A (zh) * | 2015-09-09 | 2015-12-09 | 齐鲁工业大学 | 枯草芽孢杆菌芽孢衣壳蛋白Cot表面展示海藻糖合酶的方法 |
Non-Patent Citations (5)
Title |
---|
YI LIU,等: "Efficient Production of D‑Tagatose Using a Food-Grade Surface Display System", 《JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY》 * |
余小霞,等: "枯草芽胞杆菌芽胞表面展示外源蛋白的研究进展", 《中国农业科技导报》 * |
徐铮,等: "生物法生产D-塔格糖的研究进展", 《生物加工过程》 * |
朱婧斐: "L-阿拉伯糖异构酶在枯草芽孢杆菌中的芽孢表面展示及酶学性质研究", 《中国优秀硕士学位论文全文数据库》 * |
王贺,等: "利用枯草芽孢衣壳蛋白表面展示β-半乳糖苷酶", 《食品与发酵工业》 * |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN112852702A (zh) * | 2021-03-16 | 2021-05-28 | 江南大学 | 一种重组枯草芽孢杆菌催化高浓度乳糖合成塔格糖的方法 |
CN112852702B (zh) * | 2021-03-16 | 2022-10-11 | 江南大学 | 一种重组枯草芽孢杆菌催化高浓度乳糖合成塔格糖的方法 |
CN115161310A (zh) * | 2022-08-19 | 2022-10-11 | 大连工业大学 | 一种d-半乳糖异构催化剂及其制备和应用 |
Also Published As
Publication number | Publication date |
---|---|
CN107937454B (zh) | 2020-05-01 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
Jayamuthunagai et al. | D-Tagatose production by permeabilized and immobilized Lactobacillus plantarum using whey permeate | |
Lu et al. | Synthesis of galactooligosaccharides by CBD fusion β-galactosidase immobilized on cellulose | |
CN107988286B (zh) | 一种全细胞催化制备塔格糖的方法 | |
KR101944103B1 (ko) | 마이크로박테리움 속 균주 및 이를 이용한 사이코스 생산방법 | |
CN106148265B (zh) | 一种产硫酸软骨素裂解酶的重组枯草芽孢杆菌及其应用 | |
CN107937454A (zh) | 一种固定化酶催化剂生物合成d‑塔格糖的方法 | |
WO2022148008A1 (zh) | 产塔格糖的枯草芽孢杆菌基因工程菌及制备塔格糖的方法 | |
CN104962546B (zh) | 一种含葡萄糖异构酶细胞的固定化方法 | |
CN108060114A (zh) | 一种发酵生产l-丙氨酸的大肠杆菌及其应用 | |
WO2021244005A1 (zh) | 阿洛酮糖3-差向异构酶突变体、表达其的工程菌及其固定化酶和固定化方法 | |
CN101768581B (zh) | 具有高产d-塔格糖能力的l-阿拉伯糖异构酶的突变体酶l20a及其突变方法 | |
CN107287179A (zh) | 一种褐藻胶裂解酶及其应用 | |
Jiang et al. | One-step bioprocess of inulin to product inulo-oligosaccharides using Bacillus subtilis secreting an extracellular endo-inulinase | |
CN103865863A (zh) | 一株明串珠菌突变菌株及其构建方法和应用方法 | |
CN107418903B (zh) | 一种表达葡萄糖氧化酶的低聚果糖合成工程菌株及其构建方法与应用 | |
CN112342232A (zh) | 一种适合二糖苷转移功能的重组右旋糖酐蔗糖酶大肠杆菌的构建方法 | |
CN107881140A (zh) | 一株高产甘露醇的肠膜明串珠菌突变菌株及其应用方法 | |
Sun et al. | Construction and expression of a polycistronic plasmid encoding N-acetylglucosamine 2-epimerase and N-acetylneuraminic acid lyase simultaneously for production of N-acetylneuraminic acid | |
CN105602921B (zh) | 一种壳聚糖酶突变体 | |
EP3633023A1 (en) | Strain in microbacterium and method for producing psicose using same | |
CN108060203B (zh) | 一种全细胞混合转化甘油生产1,3-丙二醇的方法 | |
CN107400667B (zh) | 一种含重组耐高温葡萄糖异构酶细胞的固定化方法 | |
CN112852702B (zh) | 一种重组枯草芽孢杆菌催化高浓度乳糖合成塔格糖的方法 | |
CN106119235B (zh) | 一种来源于伯克霍尔德氏菌的dpe及其应用 | |
CN104651332B (zh) | 嗜热细菌海藻糖合酶c端片段提高海藻糖合酶酶活的方法 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
GR01 | Patent grant | ||
GR01 | Patent grant | ||
TR01 | Transfer of patent right | ||
TR01 | Transfer of patent right |
Effective date of registration: 20230725 Address after: No. 4, Runzhou Garden Area 2, Runzhou District, Zhenjiang City, Jiangsu Province, 212004 Patentee after: Zhenjiang Baitai Biotechnology Co.,Ltd. Address before: Zhenjiang City, Jiangsu Province, 212013 Jingkou District Road No. 301 Patentee before: JIANGSU University |
|
TR01 | Transfer of patent right | ||
TR01 | Transfer of patent right |
Effective date of registration: 20231206 Address after: 442700 Factory Building No. 39, Shuidu Avenue, Shuidu Industrial Park, Economic Development Zone, Danjiangkou City, Shiyan City, Hubei Province Patentee after: Tag Sugar Biotechnology Industry Development Hubei Co.,Ltd. Address before: No. 4, Runzhou Garden Area 2, Runzhou District, Zhenjiang City, Jiangsu Province, 212004 Patentee before: Zhenjiang Baitai Biotechnology Co.,Ltd. |