CN107897126A - Firefly green cultivation technique - Google Patents
Firefly green cultivation technique Download PDFInfo
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- CN107897126A CN107897126A CN201711145692.9A CN201711145692A CN107897126A CN 107897126 A CN107897126 A CN 107897126A CN 201711145692 A CN201711145692 A CN 201711145692A CN 107897126 A CN107897126 A CN 107897126A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
Abstract
The invention discloses a kind of firefly green cultivation technique, including the hatching of ovum, the cultivation of larval phase, the management process in pupa time, the cultivation of the larval phase carries out feeding using nutrient fodder.Firefly green cultivation technique of the present invention, scientific and reasonable nursing make firefly obtain sufficient balanced nutritious supplement, reduce input cost, improve the speed of growth and shorten culturing time, the economic benefit of bigger is brought for raiser.
Description
Technical field
The present invention relates to firefly cultural technique field, and in particular to a kind of firefly green cultivation technique.
Background technology
Firefly (English：Firefly) also known as noctilucence, red-spotted stonecrop, such as it is bright credit, night shine, stream firefly, night candle, credit night, belong to elytrum
Mesh Rhagphthalmidae, is a kind of small-sized beetle, and because its afterbody can send fluorescent, therefore named is firefly.The insect that this afterbody can shine, I
State it is more typical have black firefly, Ji lycid, the window chest firefly etc. several.
Firefly is the common name of Rhagphthalmidae insect, and about 2000 kinds of the whole world, is distributed in the torrid zone, subtropical zone and Temperate Region in China.Root
According to several experts of China statistics it is now discovered that species there are about more than 100 and plant, along with undiscovered species, a total of kind more than 150.
Night will shine, and can be divided into aquatic two kinds of class and terrestrial class.Build is as low as medium-sized, long and flat, and body wall is soft with elytrum.Shirtfront
Backboard is flat, often covers head.It is narrow and small.Eye semicircle is spherical, and male eye is often greater than female.Belly 7-8 is saved, and is had below end
Photophore, the yellow-green fluorescence generated after internal fluorescein and luciferase reaction.
There is a kind of luminescent cell in the photophore of firefly, there is a kind of chemical substance containing squama in luminescent cell, be known as firefly
Light element.It will be chemically reacted when light of firefly element is run into, so as to produce radiance.Different firefly radiance durations are not
Together, for some less than one second, what is had can maintain several minutes.
Firefly nocturnalism, ovum, larva and pupa also tend to shine, and adult, which shines, lures the opposite sex.Larva
Snail and small insect are preyed on, happiness is dwelt in the place that damp warm vegetation is grown prosperity.
Adult only feeds some dew or pollen and Carnis Limax.It will be looked for when firefly will produce in hodmadod resides in
Face, and to have no the snail of defence capability as food.Scientific investigations showed that also there is a kind of firefly, being will be by eating up male firefly
What fireworm survived to multiply and protect offspring.This " fatal sweet heart " there is presently no in China's discovery, their lives mostly
In North America.They are unlike the firefly adult of China, all one's life not feeding, or only eat pollen and dew etc., they
It is the episite of standard.This firefly can be male come " luring " by imitating the female flash of light of other species fireflies, when
Male firefly for oneself to pay court to after obtaining response, when coming to date, will be eaten up by other side.
The content of the invention
Existing in the prior art in order to solve the problems, such as, the present invention provides a kind of firefly green cultivation technique.
Technical solution is as follows：
A kind of firefly green cultivation technique, including the hatching of ovum, the cultivation of larval phase, the management process in pupa time.It is described
The cultivation of larval phase carries out feeding using nutrient fodder.
The preparation method of the nutrient fodder, comprises the following steps：
(1) snail is shelled, takes Carnis Limax to wash with water, removed silt, dry, crushed 1-5 mesh sieves, obtain coarse crushing
Carnis Limax afterwards；Carnis Limax after coarse crushing is dried in vacuo, then crushed 15-25 mesh sieves, obtain it is finely divided after snail
Beef；Carnis Limax after will be finely divided is dried in vacuo, and obtains snail digested tankage；
(2) river snail is shelled, takes spiral shell to wash with water, removed silt, dry, crushed 1-5 mesh sieves, obtain coarse crushing
Spiral shell afterwards；Spiral shell after coarse crushing is dried in vacuo, then crushed 15-25 mesh sieves, obtain it is finely divided after field
Spiral shell meat；Spiral shell after will be finely divided is dried in vacuo, and obtains river snail digested tankage；
(3) conch is shelled, takes sea snail meat to wash with water, removed silt, dry, crushed 1-5 mesh sieves, obtain coarse crushing
Sea snail meat afterwards；Sea snail meat after coarse crushing is dried in vacuo, then crushed 15-25 mesh sieves, obtain it is finely divided after sea
Spiral shell meat；Sea snail meat after will be finely divided is dried in vacuo, and obtains conch digested tankage；
(4) take 55-65 parts by weight snails digested tankage, 25-35 parts by weight river snail digested tankage, 5-15 parts by weight conches digested tankage,
0.005-0.02 parts by weight sodium sorbate, 0.2-0.9 parts by weight chitosan, 0.01-0.05 parts by weight antistaling agents are uniformly mixed, and are gone out
Bacterium, to obtain the final product.
Preferably, the preparation method of the nutrient fodder, comprises the following steps：
(1) snail is shelled, takes Carnis Limax to wash with water, removed silt, dry, crushed 1-5 mesh sieves, obtain coarse crushing
Carnis Limax afterwards；Carnis Limax after coarse crushing is dried in vacuo, then crushed 15-25 mesh sieves, obtain it is finely divided after snail
Beef；Carnis Limax after will be finely divided is dried in vacuo, and obtains snail digested tankage；
(2) river snail is shelled, takes spiral shell to wash with water, removed silt, dry, crushed 1-5 mesh sieves, obtain coarse crushing
Spiral shell afterwards；Spiral shell after coarse crushing is dried in vacuo, then crushed 15-25 mesh sieves, obtain it is finely divided after field
Spiral shell meat；Spiral shell after will be finely divided is dried in vacuo, and obtains river snail digested tankage；
(3) conch is shelled, takes sea snail meat to wash with water, removed silt, dry, crushed 1-5 mesh sieves, obtain coarse crushing
Sea snail meat afterwards；Sea snail meat after coarse crushing is dried in vacuo, then crushed 15-25 mesh sieves, obtain it is finely divided after sea
Spiral shell meat；Sea snail meat after will be finely divided is dried in vacuo, and obtains conch digested tankage；
(4) take 55-65 parts by weight snails digested tankage, 25-35 parts by weight river snail digested tankage, 5-15 parts by weight conches digested tankage,
0.005-0.02 parts by weight sodium sorbate, the part by weight modified chitosans of 0.2-0.9, the mixing of 0.01-0.05 parts by weight antistaling agent are equal
It is even, sterilizing, to obtain the final product.
The preparation method of the modification of chitosan is：It is dilute for 0.0005-0.0015mol/L that chitosan is dissolved in molar concentration
The chitosan solution that mass fraction is 1-2% is configured in hydrochloric acid, is 1- by the sodium alginate mass fraction soluble in water that is configured to
3% sodium alginate soln；It is in mass ratio by chitosan solution, sodium alginate soln (9-13)：1 using rotating speed as 200-500
Rev/min stirring 5-15min be uniformly mixed, add mass concentration be 1-2% calcium chloride water, the calcium chloride water
Addition be sodium alginate soln quality 50-70%, using rotating speed for 200-500 revs/min stir 5-15min be uniformly mixed,
PH value is adjusted to 6.5-7.5 with the sodium hydrate aqueous solution of 0.005-0.015mol/L, after solid floccule to appear, is stood
12-36h, filtering, dries 30-40h at 35-40 DEG C, obtains modification of chitosan.
The antistaling agent is fumaric acid galactolipin methyl esters and/or glucosyl methyl fumarate.In an embodiment party of the invention
In formula, the antistaling agent is made of 75-85wt% fumaric acid galactolipin methyl esters and 15-25wt% glucosyl methyl fumarates.
The vacuum drying vacuum is 0.05-0.15MPa, and temperature is 40-60 DEG C, time 3-7h.
The sterilizing is microwave sterilization or ultraviolet sterilization.
The microwave power of the microwave sterilization is 500-700W, microwave frequency 2400-2500MHz, time 1-4min.
Firefly green cultivation technique of the present invention, scientific and reasonable nursing make firefly obtain sufficient balanced nutritious supplement,
Input cost is reduced, the speed of growth is improved and shortens culturing time, the economic benefit of bigger is brought for raiser.
Embodiment
Protein lost is tested：Nutrient fodder is placed in the environment that temperature is 25 ± 1 DEG C, relative humidity is 60 ± 5%
Under be sealed 6 months.The protein content of nutrient fodder before and after preservation is tested, it is triumphant with reference to GB/T 5009.5-1985
Family name's nitriding carries out, and the model ATN-300's that test equipment is provided using Shanghai flood discipline experimental instruments and equipment limited is full-automatic
Kjeldahl apparatus.Protein lost=[1- (protein content before protein content/preservation after preservation)] × 100%.
Antiseptic property is tested：Nutrient fodder is placed in temperature as 40 ± 1 DEG C, relative humidity opens in the environment of being 85 ± 5%
Storage is opened, staphylococcus aureus (ATCC 6538) total plate count and Escherichia coli (ATYCC 25922) bacterium colony are carried out after 30 days
Sum test, with reference to GBT 4789.2-2008 microbiological test of food hygiene total plate count measure.
River snail is the artificial breeding river snail that Shen Nongshi special cultivations Specialty Co-operative Organization of Xinfeng County provides in embodiment.
The wild big conch that conch provides for Dandong Tai Hong Food Co., Ltd in embodiment, original producton location Liaoning.
Snail is that snail white jade snail cultivates the artificial breeding white jade snail that Specialty Co-operative Organization provides in Xiangshui County in embodiment.
The food-grade chitosan that chitosan provides for Hunan century Hua Xing bioengineering Co., Ltd in embodiment.
Sodium sorbate in embodiment, No. CAS：7757-81-5.
Paper of the fumaric acid galactolipin methyl esters with reference to Zhang Qing, Zhong Liai, Liang Hongdong, week such as golden hair table in embodiment《It is new
The synthesis of preservative fumaric acid galactolipin methyl esters and Antibacterial Property》Synthesized.
The paper that glucosyl methyl fumarate is delivered with reference to Zhang Qing, Zhou Rujin, Jin Feng, beam wild goose Chuang in embodiment《2 kinds of rich horses
Phase-transfer Wittig reaction and the bacteriostatic activity research of sour sugar ester》Synthesized.
Sodium alginate is the food-grade sodium alginate that one hundred emerging bio tech ltd of Wuhan provides in embodiment.
Calcium chloride in embodiment, No. CAS：10043-52-4.
Sodium hydroxide in embodiment, No. CAS：1310-73-2.
Embodiment 1
Firefly green cultivation technique, including：
The hatching of S1, ovum：After the thorough dried disinfecting of loam, scattered ovum grain is placed in the pitting on soil, above may be used
Covered with covering the wet gauze of some upper disinfections or toilet paper, it is desirable to which loam remains 15% or so humidity, loam
On to have more insulating space, allow ovum to be individually insulated, by above-mentioned ovum and hatching utensil and matrix etc. natural shape disposed within
Hatch under state, after the completion of hatching, larva is transferred in breeding box；
The cultivation of S2, larval phase：By nutrient fodder be placed on filter paper or other small appliances in, be placed in above the water surface
Dry place, allows larva fixed point feeding, throws away filter paper after feeding, cleans the utensils such as culture dish, in order to avoid pollute or mouldy
Catch an illness；
S3, the management process in pupa time：When vault space firefly grows to mature larva, larva to be inhabited inside breeding box
Place of pupating is built in region outside object, and specific building course is：Cohesive soil after thickness is disinfected for 2-4cm or so
Earth is layered on cassette bottom, adds water to be tuned into water content 20-40% or so, and all kinds of stones such as some cobblestones are randomly placed on soil, supplies
Mature larva rank soil is pupated on stone.
The preparation method of the nutrient fodder, comprises the following steps：
(1) snail is shelled, takes Carnis Limax to wash with water, remove silt, placed 20min and dry, crushed 2 mesh sieves, obtain
Carnis Limax after to coarse crushing；In vacuum it is 0.1MPa by the Carnis Limax after coarse crushing, temperature is dried in vacuo for 50 DEG C
5h, then crushed 20 mesh sieves, obtain it is finely divided after Carnis Limax；Carnis Limax after will be finely divided is 0.1MPa in vacuum, temperature
Spend and carry out vacuum drying 5h for 50 DEG C, obtain snail digested tankage；
(2) river snail is shelled, takes spiral shell to wash with water, remove silt, placed 20min and dry, crushed 2 mesh sieves, obtain
Spiral shell after to coarse crushing；In vacuum it is 0.1MPa by the spiral shell after coarse crushing, temperature is dried in vacuo for 50 DEG C
5h, then crushed 20 mesh sieves, obtain it is finely divided after spiral shell；Spiral shell after will be finely divided is 0.1MPa in vacuum, temperature
Spend and carry out vacuum drying 5h for 50 DEG C, obtain river snail digested tankage；
(3) conch is shelled, takes sea snail meat to wash with water, remove silt, placed 20min and dry, crushed 2 mesh sieves, obtain
Sea snail meat after to coarse crushing；In vacuum it is 0.1MPa by the sea snail meat after coarse crushing, temperature is dried in vacuo for 50 DEG C
5h, then crushed 20 mesh sieves, obtain it is finely divided after sea snail meat；Sea snail meat after will be finely divided is 0.1MPa in vacuum, temperature
Spend and carry out vacuum drying 5h for 50 DEG C, obtain conch digested tankage；
(4) 60 parts by weight snail digested tankages, 30 parts by weight river snail digested tankages, 10 parts by weight conch digested tankages, 0.01 parts by weight mountain are taken
Pears acid sodium, 0.7 part by weight modified chitosan, 0.02 parts by weight fumaric acid galactolipin methyl esters are using rotating speed as 150 revs/min of stirrings
10min is uniformly mixed, microwave power be 600W, microwave frequency be 2450MHz microwave sterilization 2min, up to nutrient fodder.
The preparation method of the modification of chitosan is：Chitosan is dissolved in molar concentration to match somebody with somebody in 0.001mol/L dilute hydrochloric acid
The chitosan solution that mass fraction is 1.5% is made, by the sodium alginate mass fraction soluble in water that is configured to for 2% alginic acid
Sodium solution；It is 11 in mass ratio by chitosan solution, sodium alginate soln：1 is equal as 300 revs/min of stirring 10min mixing using rotating speed
It is even, the calcium chloride water that mass concentration is 1.5% is added, the addition of the calcium chloride water is sodium alginate soln
The 60% of quality, is uniformly mixed by 300 revs/min of stirring 10min of rotating speed, is adjusted with the sodium hydrate aqueous solution of 0.01mol/L
PH value is to 6.5-7.5, after solid floccule to appear, stands 24h, using 300 mesh filter-cloth filterings, dries 36h at 38 DEG C, obtains
Modification of chitosan.
Embodiment 2
Firefly green cultivation technique, including：
The hatching of S1, ovum：After the thorough dried disinfecting of loam, scattered ovum grain is placed in the pitting on soil, above may be used
Covered with covering the wet gauze of some upper disinfections or toilet paper, it is desirable to which loam remains 15% or so humidity, loam
On to have more insulating space, allow ovum to be individually insulated, by above-mentioned ovum and hatching utensil and matrix etc. natural shape disposed within
Hatch under state, after the completion of hatching, larva is transferred in breeding box；
The cultivation of S2, larval phase：By nutrient fodder be placed on filter paper or other small appliances in, be placed in above the water surface
Dry place, allows larva fixed point feeding, throws away filter paper after feeding, cleans the utensils such as culture dish, in order to avoid pollute or mouldy
Catch an illness；
S3, the management process in pupa time：When vault space firefly grows to mature larva, larva to be inhabited inside breeding box
Place of pupating is built in region outside object, and specific building course is：Cohesive soil after thickness is disinfected for 2-4cm or so
Earth is layered on cassette bottom, adds water to be tuned into water content 20-40% or so, and all kinds of stones such as some cobblestones are randomly placed on soil, supplies
Mature larva rank soil is pupated on stone.
The preparation method of the nutrient fodder, comprises the following steps：
(1) snail is shelled, takes Carnis Limax to wash with water, remove silt, placed 20min and dry, crushed 2 mesh sieves, obtain
Carnis Limax after to coarse crushing；In vacuum it is 0.1MPa by the Carnis Limax after coarse crushing, temperature is dried in vacuo for 50 DEG C
5h, then crushed 20 mesh sieves, obtain it is finely divided after Carnis Limax；Carnis Limax after will be finely divided is 0.1MPa in vacuum, temperature
Spend and carry out vacuum drying 5h for 50 DEG C, obtain snail digested tankage；
(2) river snail is shelled, takes spiral shell to wash with water, remove silt, placed 20min and dry, crushed 2 mesh sieves, obtain
Spiral shell after to coarse crushing；In vacuum it is 0.1MPa by the spiral shell after coarse crushing, temperature is dried in vacuo for 50 DEG C
5h, then crushed 20 mesh sieves, obtain it is finely divided after spiral shell；Spiral shell after will be finely divided is 0.1MPa in vacuum, temperature
Spend and carry out vacuum drying 5h for 50 DEG C, obtain river snail digested tankage；
(3) conch is shelled, takes sea snail meat to wash with water, remove silt, placed 20min and dry, crushed 2 mesh sieves, obtain
Sea snail meat after to coarse crushing；In vacuum it is 0.1MPa by the sea snail meat after coarse crushing, temperature is dried in vacuo for 50 DEG C
5h, then crushed 20 mesh sieves, obtain it is finely divided after sea snail meat；Sea snail meat after will be finely divided is 0.1MPa in vacuum, temperature
Spend and carry out vacuum drying 5h for 50 DEG C, obtain conch digested tankage；
(4) 60 parts by weight snail digested tankages, 30 parts by weight river snail digested tankages, 10 parts by weight conch digested tankages, 0.01 parts by weight mountain are taken
Pears acid sodium, 0.7 parts by weight chitosan, 0.02 parts by weight fumaric acid galactolipin methyl esters are mixed by 150 revs/min of stirring 10min of rotating speed
Close it is uniform, microwave power be 600W, microwave frequency be 2450MHz microwave sterilization 2min, up to nutrient fodder.
Embodiment 3
Firefly green cultivation technique, including：
The hatching of S1, ovum：After the thorough dried disinfecting of loam, scattered ovum grain is placed in the pitting on soil, above may be used
Covered with covering the wet gauze of some upper disinfections or toilet paper, it is desirable to which loam remains 15% or so humidity, loam
On to have more insulating space, allow ovum to be individually insulated, by above-mentioned ovum and hatching utensil and matrix etc. natural shape disposed within
Hatch under state, after the completion of hatching, larva is transferred in breeding box；
The cultivation of S2, larval phase：By nutrient fodder be placed on filter paper or other small appliances in, be placed in above the water surface
Dry place, allows larva fixed point feeding, throws away filter paper after feeding, cleans the utensils such as culture dish, in order to avoid pollute or mouldy
Catch an illness；
S3, the management process in pupa time：When vault space firefly grows to mature larva, larva to be inhabited inside breeding box
Place of pupating is built in region outside object, and specific building course is：Cohesive soil after thickness is disinfected for 2-4cm or so
Earth is layered on cassette bottom, adds water to be tuned into water content 20-40% or so, and all kinds of stones such as some cobblestones are randomly placed on soil, supplies
Mature larva rank soil is pupated on stone.
The preparation method of the nutrient fodder, comprises the following steps：
(1) snail is shelled, takes Carnis Limax to wash with water, remove silt, placed 20min and dry, crushed 2 mesh sieves, obtain
Carnis Limax after to coarse crushing；In vacuum it is 0.1MPa by the Carnis Limax after coarse crushing, temperature is dried in vacuo for 50 DEG C
5h, then crushed 20 mesh sieves, obtain it is finely divided after Carnis Limax；Carnis Limax after will be finely divided is 0.1MPa in vacuum, temperature
Spend and carry out vacuum drying 5h for 50 DEG C, obtain snail digested tankage；
(2) river snail is shelled, takes spiral shell to wash with water, remove silt, placed 20min and dry, crushed 2 mesh sieves, obtain
Spiral shell after to coarse crushing；In vacuum it is 0.1MPa by the spiral shell after coarse crushing, temperature is dried in vacuo for 50 DEG C
5h, then crushed 20 mesh sieves, obtain it is finely divided after spiral shell；Spiral shell after will be finely divided is 0.1MPa in vacuum, temperature
Spend and carry out vacuum drying 5h for 50 DEG C, obtain river snail digested tankage；
(3) conch is shelled, takes sea snail meat to wash with water, remove silt, placed 20min and dry, crushed 2 mesh sieves, obtain
Sea snail meat after to coarse crushing；In vacuum it is 0.1MPa by the sea snail meat after coarse crushing, temperature is dried in vacuo for 50 DEG C
5h, then crushed 20 mesh sieves, obtain it is finely divided after sea snail meat；Sea snail meat after will be finely divided is 0.1MPa in vacuum, temperature
Spend and carry out vacuum drying 5h for 50 DEG C, obtain conch digested tankage；
(4) 60 parts by weight snail digested tankages, 30 parts by weight river snail digested tankages, 10 parts by weight conch digested tankages, 0.01 parts by weight mountain are taken
Pears acid sodium, 0.7 part by weight modified chitosan, 0.02 parts by weight glucosyl methyl fumarate are using rotating speed as 150 revs/min of stirrings
10min is uniformly mixed, microwave power be 600W, microwave frequency be 2450MHz microwave sterilization 2min, up to nutrient fodder.
The preparation method of the modification of chitosan is：Chitosan is dissolved in molar concentration to match somebody with somebody in 0.001mol/L dilute hydrochloric acid
The chitosan solution that mass fraction is 1.5% is made, by the sodium alginate mass fraction soluble in water that is configured to for 2% alginic acid
Sodium solution；It is 11 in mass ratio by chitosan solution, sodium alginate soln：1 is equal as 300 revs/min of stirring 10min mixing using rotating speed
It is even, the calcium chloride water that mass concentration is 1.5% is added, the addition of the calcium chloride water is sodium alginate soln
The 60% of quality, is uniformly mixed by 300 revs/min of stirring 10min of rotating speed, is adjusted with the sodium hydrate aqueous solution of 0.01mol/L
PH value is to 6.5-7.5, after solid floccule to appear, stands 24h, using 300 mesh filter-cloth filterings, dries 36h at 38 DEG C, obtains
Modification of chitosan.
Embodiment 4
Firefly green cultivation technique, including：
The hatching of S1, ovum：After the thorough dried disinfecting of loam, scattered ovum grain is placed in the pitting on soil, above may be used
Covered with covering the wet gauze of some upper disinfections or toilet paper, it is desirable to which loam remains 15% or so humidity, loam
On to have more insulating space, allow ovum to be individually insulated, by above-mentioned ovum and hatching utensil and matrix etc. natural shape disposed within
Hatch under state, after the completion of hatching, larva is transferred in breeding box；
The cultivation of S2, larval phase：By nutrient fodder be placed on filter paper or other small appliances in, be placed in above the water surface
Dry place, allows larva fixed point feeding, throws away filter paper after feeding, cleans the utensils such as culture dish, in order to avoid pollute or mouldy
Catch an illness；
S3, the management process in pupa time：When vault space firefly grows to mature larva, larva to be inhabited inside breeding box
Place of pupating is built in region outside object, and specific building course is：Cohesive soil after thickness is disinfected for 2-4cm or so
Earth is layered on cassette bottom, adds water to be tuned into water content 20-40% or so, and all kinds of stones such as some cobblestones are randomly placed on soil, supplies
Mature larva rank soil is pupated on stone.
The preparation method of the nutrient fodder, comprises the following steps：
(1) snail is shelled, takes Carnis Limax to wash with water, remove silt, placed 20min and dry, crushed 2 mesh sieves, obtain
Carnis Limax after to coarse crushing；In vacuum it is 0.1MPa by the Carnis Limax after coarse crushing, temperature is dried in vacuo for 50 DEG C
5h, then crushed 20 mesh sieves, obtain it is finely divided after Carnis Limax；Carnis Limax after will be finely divided is 0.1MPa in vacuum, temperature
Spend and carry out vacuum drying 5h for 50 DEG C, obtain snail digested tankage；
(2) river snail is shelled, takes spiral shell to wash with water, remove silt, placed 20min and dry, crushed 2 mesh sieves, obtain
Spiral shell after to coarse crushing；In vacuum it is 0.1MPa by the spiral shell after coarse crushing, temperature is dried in vacuo for 50 DEG C
5h, then crushed 20 mesh sieves, obtain it is finely divided after spiral shell；Spiral shell after will be finely divided is 0.1MPa in vacuum, temperature
Spend and carry out vacuum drying 5h for 50 DEG C, obtain river snail digested tankage；
(3) conch is shelled, takes sea snail meat to wash with water, remove silt, placed 20min and dry, crushed 2 mesh sieves, obtain
Sea snail meat after to coarse crushing；In vacuum it is 0.1MPa by the sea snail meat after coarse crushing, temperature is dried in vacuo for 50 DEG C
5h, then crushed 20 mesh sieves, obtain it is finely divided after sea snail meat；Sea snail meat after will be finely divided is 0.1MPa in vacuum, temperature
Spend and carry out vacuum drying 5h for 50 DEG C, obtain conch digested tankage；
(4) 60 parts by weight snail digested tankages, 30 parts by weight river snail digested tankages, 10 parts by weight conch digested tankages, 0.01 parts by weight mountain are taken
Pears acid sodium, 0.7 part by weight modified chitosan, 0.016 parts by weight fumaric acid galactolipin methyl esters, 0.004 parts by weight fumaric acid grape
Sugared methyl esters using rotating speed as 150 revs/min stirring 10min be uniformly mixed, microwave power be 600W, microwave frequency be 2450MHz micro-
Ripple sterilizing 2min, up to nutrient fodder.Nutrient fodder is subjected to protein lost, antiseptic property test, test result：Albumen
Matter loss late is 0.93%, and S. aureus colonies sum is 2.7 × 102Cfu/g, E. coli clones sum are 5.4
×102cfu/g。
The preparation method of the modification of chitosan is：Chitosan is dissolved in molar concentration to match somebody with somebody in 0.001mol/L dilute hydrochloric acid
The chitosan solution that mass fraction is 1.5% is made, by the sodium alginate mass fraction soluble in water that is configured to for 2% alginic acid
Sodium solution；It is 11 in mass ratio by chitosan solution, sodium alginate soln：1 is equal as 300 revs/min of stirring 10min mixing using rotating speed
It is even, the calcium chloride water that mass concentration is 1.5% is added, the addition of the calcium chloride water is sodium alginate soln
The 60% of quality, is uniformly mixed by 300 revs/min of stirring 10min of rotating speed, is adjusted with the sodium hydrate aqueous solution of 0.01mol/L
PH value is to 6.5-7.5, after solid floccule to appear, stands 24h, using 300 mesh filter-cloth filterings, dries 36h at 38 DEG C, obtains
Modification of chitosan.
Test case 1
Nutrient fodder is subjected to protein lost, antiseptic property test, concrete outcome is shown in Table 1.
Table 1：Test result table
By using to chitin modified, nutrient fodder quality is largely increased the embodiment of the present invention 1, its reason may
After being chitin modified, its anti-oxidant, bacteriostasis is improved.
Claims (8)
1. a kind of firefly green cultivation technique, including the hatching of ovum, the cultivation of larval phase, the management process in pupa time, its feature
It is, the cultivation of the larval phase carries out feeding using nutrient fodder.
2. firefly green cultivation technique as claimed in claim 1, it is characterised in that the preparation method of the nutrient fodder,
Comprise the following steps：
(1) snail is shelled, takes Carnis Limax to wash with water, removed silt, dry, 1-5 mesh sieves are crushed, after obtaining coarse crushing
Carnis Limax；Carnis Limax after coarse crushing is dried in vacuo, then crushed 15-25 mesh sieves, obtain it is finely divided after Carnis Limax；
Carnis Limax after will be finely divided is dried in vacuo, and obtains snail digested tankage；
(2) river snail is shelled, takes spiral shell to wash with water, removed silt, dry, 1-5 mesh sieves are crushed, after obtaining coarse crushing
Spiral shell；Spiral shell after coarse crushing is dried in vacuo, then crushed 15-25 mesh sieves, obtain it is finely divided after spiral shell；
Spiral shell after will be finely divided is dried in vacuo, and obtains river snail digested tankage；
(3) conch is shelled, takes sea snail meat to wash with water, removed silt, dry, 1-5 mesh sieves are crushed, after obtaining coarse crushing
Sea snail meat；Sea snail meat after coarse crushing is dried in vacuo, then crushed 15-25 mesh sieves, obtain it is finely divided after sea snail meat；
Sea snail meat after will be finely divided is dried in vacuo, and obtains conch digested tankage；
(4) 55-65 parts by weight snails digested tankage, 25-35 parts by weight river snail digested tankage, 5-15 parts by weight conches digested tankage, 0.005- are taken
0.02 parts by weight sodium sorbate, 0.2-0.9 parts by weight chitosan, 0.01-0.05 parts by weight antistaling agents are uniformly mixed, sterilizing, i.e.,
.
3. firefly green cultivation technique as claimed in claim 1, it is characterised in that the preparation method of the nutrient fodder,
Comprise the following steps：
(1) snail is shelled, takes Carnis Limax to wash with water, removed silt, dry, 1-5 mesh sieves are crushed, after obtaining coarse crushing
Carnis Limax；Carnis Limax after coarse crushing is dried in vacuo, then crushed 15-25 mesh sieves, obtain it is finely divided after Carnis Limax；
Carnis Limax after will be finely divided is dried in vacuo, and obtains snail digested tankage；
(2) river snail is shelled, takes spiral shell to wash with water, removed silt, dry, 1-5 mesh sieves are crushed, after obtaining coarse crushing
Spiral shell；Spiral shell after coarse crushing is dried in vacuo, then crushed 15-25 mesh sieves, obtain it is finely divided after spiral shell；
Spiral shell after will be finely divided is dried in vacuo, and obtains river snail digested tankage；
(3) conch is shelled, takes sea snail meat to wash with water, removed silt, dry, 1-5 mesh sieves are crushed, after obtaining coarse crushing
Sea snail meat；Sea snail meat after coarse crushing is dried in vacuo, then crushed 15-25 mesh sieves, obtain it is finely divided after sea snail meat；
Sea snail meat after will be finely divided is dried in vacuo, and obtains conch digested tankage；
(4) 55-65 parts by weight snails digested tankage, 25-35 parts by weight river snail digested tankage, 5-15 parts by weight conches digested tankage, 0.005- are taken
0.02 parts by weight sodium sorbate, the part by weight modified chitosans of 0.2-0.9,0.01-0.05 parts by weight antistaling agents are uniformly mixed, and are gone out
Bacterium, to obtain the final product.
4. firefly green cultivation technique as claimed in claim 3, it is characterised in that the preparation method of the modification of chitosan
For：It is that the shell that mass fraction is 1-2% is configured in 0.0005-0.0015mol/L dilute hydrochloric acid that chitosan is dissolved in molar concentration
Glycan solution, by sodium alginate it is soluble in water be configured to mass fraction be 1-3% sodium alginate soln；By chitosan solution,
Sodium alginate soln is (9-13) in mass ratio：1 is uniformly mixed by 200-500 revs/min of stirring 5-15min of rotating speed, adds
Mass concentration is the calcium chloride water of 1-2%, and the addition of the calcium chloride water is the 50- of sodium alginate soln quality
70%, it is uniformly mixed by 200-500 revs/min of stirring 5-15min of rotating speed, it is water-soluble with the sodium hydroxide of 0.005-0.015mol/L
Liquid adjusts pH value to 6.5-7.5, after solid floccule to appear, stands 12-36h, filtering, dries 30-40h at 35-40 DEG C, obtain
To modification of chitosan.
5. firefly green cultivation technique as claimed in claim 3, it is characterised in that：The antistaling agent is fumaric acid galactolipin
Methyl esters and/or glucosyl methyl fumarate.
6. firefly green cultivation technique as claimed in claim 3, it is characterised in that：The vacuum drying vacuum is
0.05-0.15MPa, temperature are 40-60 DEG C, time 3-7h.
7. firefly green cultivation technique as claimed in claim 3, it is characterised in that：The sterilizing is microwave sterilization or ultraviolet
Sterilizing.
8. firefly green cultivation technique as claimed in claim 7, it is characterised in that：The microwave power of the microwave sterilization is
500-700W, microwave frequency 2400-2500MHz, time 1-4min.
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| CN111066981A (en) * | 2019-12-12 | 2020-04-28 | 吴爱芳 | Special feed for firefly and application of special feed to efficient firefly breeding |
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| CN201138994Y (en) * | 2007-08-24 | 2008-10-29 | 邰港科技股份有限公司 | Inspection box for raising firefly |
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