CN101300962B - Cultivation method of blowfish with controlled toxicity - Google Patents

Cultivation method of blowfish with controlled toxicity Download PDF

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CN101300962B
CN101300962B CN2008100116623A CN200810011662A CN101300962B CN 101300962 B CN101300962 B CN 101300962B CN 2008100116623 A CN2008100116623 A CN 2008100116623A CN 200810011662 A CN200810011662 A CN 200810011662A CN 101300962 B CN101300962 B CN 101300962B
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fish
toxicity
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CN101300962A (en
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刘烈
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish

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Abstract

The present invention relates to a toxicity controlling cultivation method of blowfish, which is based on the mechanism that the fugutoxin is an exogenous toxin and is formed by exchanging with toxic substance in living environment during growing course, and which is aimed at controlling the environmental factors and food factors forming fugutoxin, thereby the toxin inside the blowfish body can be reduced to meet the safety edible standard. The toxicity controlling cultivation method is characterized by controlling the toxicity source of the blowfish living environment and monitoring and controlling the bait toxicity source, and cutting off and avoiding the source of the fugutoxin from environmental factors and food factors, thus leading the toxin content in blowfish body to be very little and reaching non-toxin standard in fact. The toxicity controlling cultivation method can meet the requirements of good taste, nutrition, diseases prevention and treatment and etc. under safe precondition for consumers, which provides a good and safe aquatic product for living.

Description

Cultivation method of blowfish with controlled toxicity
Technical field:
The present invention relates to the artificial cultivating method of a kind of aquaculture method, particularly a kind of globe fish.
Background technology:
Globe fish is a kind of special fingerling, and because of its delicious flavour is caught people's attention, its nutritive value is better than other aquatic products, is described as the king in the fish.According to surveying and determination, protein content reaches 18.7 grams in the every hectogram filefish flesh of fish, exceeds tens times than other marine product, and fat content only is 0.26 gram, is minimum a kind of of fat content in the fish.Globe fish also contains the trace element of multiple beneficial such as abundant vitamin B1, B12 and selenium, zinc, and the content of the nutrient of human gene---nucleic acid and brain gold content are in the biology the first, and several amino acids content is all on other fish.Because the globe fish meat is pure white, delicate, aromatic taste is tempting, so there be " emit and extremely eat filefish " to say.It is not only delicious because of it that globe fish becomes food, because its energy disease-relieving and physique enhancing often eats globe fish and can prevent and treat diseases such as cardiovascular and cerebrovascular diseases, hypertension, hepatitis, its internal organ and blood can also refine valuable medicinal raw material again.Yet, owing to illegal edible and eat poisoning, the dead case that toxic filefish and dried product thereof cause by mistake and happen occasionally, about globe fish toxicity and to the issuable serious consequence problem of eater people are not dared to inquire to it for a long time, bring serious negative effect for the globe fish industry.At present, the globe fish cultural method mainly adopts cage culture and encloses the sea and culture two kinds of methods in the world, owing to lack the malicious technology of perfect, effective control, the globe fish that existing cultural method is cultured is suitable with the globe fish toxicity of occurring in nature, and all there are certain danger in edible and processing.
Summary of the invention:
The object of the present invention is to provide a kind of cultivation method of blowfish with controlled toxicity that in the globe fish breeding process, can effectively control the globe fish vivotoxin, guarantee its edible safety.
The present invention realizes like this.It is heterotoxin that this kind cultivation method of blowfish with controlled toxicity is based on the globe fish toxin, be in the globe fish process of growth by with living environment in this mechanism of forming of noxious material exchange, the environmental factor and the food factor that form at the globe fish toxin in the globe fish breeding process are controlled, can be for the standard of safe edible thereby its vivotoxin content is reduced to.This cultural method is divided into outdoor ponds and cultures and marine cage culture dual mode.
Implementation step and technical essential are as follows:
1, the pond is built: pond area 3hm 2-5hm 2(hm 2-hectare), the north-south is chosen in free of contamination area and has and independently advance pumping equipment, more than the mean depth of water 2m for well;
2, the sterilization of cleaning up the pond: clean up the pond and to carry out putting seedling first half of the month, be equipped with oxygen increasing equipment, turn over rake at the bottom of to the pond, tan by the sun mid-May, make the rancid abundant oxidation Decomposition of beach mud of the melanism of wherein depositing, simultaneously per hectare is with the quicklime 2000kg sterilization of splashing at the bottom of to the pond, drain after 3 days, airing was intake after one week, and the depth of water is more than 1.5m; Adopt winter the mode of dry freeze, weathering to make at the bottom of the pond gradually to low ammonia nitrogen index recovery, disappear, irrigation and drainage mid-March in 1 year 1-2 time, water washes away at the bottom of the pond and with Humus drains with water;
3, seed is thrown in: select kind pure, no sick and wounded, physique good, the seed of the long 50-60mm of specification homogeneous body is thrown in, at the beginning of the best release time is late May to 6 month, water temperature is more than 15 ℃, injected volume be one age 600-800 tail/mu or two age 30-50 tail/mu, breeding density is 3500-4500 tail/hm 2
4, feed: throwing in fresh bait such as freezing sand lance, shrimp, is auxilliary with artitificial food, and feeding volume is thrown something and fed 4-5 time by the 20%-30% of fish body total amount every day;
5, breeding condition and water quality adjustment: the pond depth of water will remain on 1.2m-1.5m, and water temperature 15-30 ℃, transparency will remain on 0.3-0.4m, intensity of illumination 500lx-1000lx; Changed water once in per 15 days, quantity of exchanged water will reach 40% of total water body, and the oxygen content in the water body remains on more than the 5mg/L, and pH value remains between the 7.8-8.3, salinity 15-32, ammonia-nitrogen content≤1mg/L, nitroso nitrogen≤0.1mg/L;
6, daily management: repeatedly patrol the pool every day, observe the active situation of water quality and fish, check that fry ingests, growth and disease, pinpoint the problems and in time handle; Carry out water quality adjustment and change the water conservancy project work; Long and the body weight of routine test seed body is checked the fry growing state, and the discovery fish disease should in time be picked out;
7, survive the winter: when water temperature is reduced to 12 ℃, move into fry indoor or survive the winter at booth, water temperature remains on more than 12 ℃, prevents freeze injury; The freezing new iced trash fish of throwing something and feeding every day once, a day feeding volume is the 2-3% of fish body weight; The pond fell in every month once, thoroughly clean and sterilize; Change water every day once, quantity of exchanged water is 50%.
8, survive the winter after, water temperature rises to 15 ℃, can shift out the outdoor breeding large size fingerling again, up to marketable fish size.
9, big specification fish culture: select the above healthy fish of body weight 150g/ tail, breeding density is 700-800 tail/hm 2, the freezing new iced trash fish of throwing something and feeding every day 2 times, a day feeding volume is the 2-3% of fish body weight.
Marine cage culture relative complex need be cut tooth and be handled before fry goes out the pond, fish is put into live fish box, is immersed in the seawater and anaesthetizes, cuts tooth, and operation can not injure fish and go up lower jaw, carries out the dipping sterilization with hydrogen peroxide; Transport back indoorly when surviving the winter from the sea, the situation that support in bait and pond is identical.Its advantage is: cultivation water environment is condition cleaning, the safety of pond culture relatively, fast growth.
Be the key point of this method to the control in filefish growing environment poison source and the Monitoring and Controlling in bait poison source in breeding process, the main practice is:
1, filefish growing environment poison source control
1. pond sterilization:
Thoroughly clean up the pond, eliminate benthic mollusca, algae, bacterium, actinomycetes, form before putting in a suitable place to breed in " inanimate object district " at the bottom of the pond.Concrete grammar is to use machine to clean up the pond before the pond water inlet in the beginning of spring, roots out mud at the bottom of the pond, throws in 1 kilogram in bleaching powder, evenly sprays sterilizing for per 5 square metres; Airing was intake after one week, was equipped with in the future to culture;
2. water quality control:
I installs apparatus for ultraviolet disinfection and ozone sterilization device at water inlet, and seawater is disinfected, and reduces the microbial poison source.Increase the seawater bacterial digestion simultaneously and cultivate, form advantage group, thereby reduce the intoxicating micro organism quantity;
II Chi Shui can produce dehydration-TTX and actinomyces thereof every several days microscopy monitoring seawater suspicious bacterial species, especially pseudomonas bacteriums in the filefish enteron aisle; The next biological calcareous algae of supervision filefish food chain, conch, decorative pattern are liked toxic biologies such as clean crab, bluish green spotted parrot fish;
The III pond depth of water will remain on 1.2m-1.5m, and transparency will remain on more than the 40cm; Once changed water in every 7-10 days, quantity of exchanged water will reach more than 30% of total water body, and the oxygen content in the water body remains on 5mg/L, and pH value remains between the 7.8-8.3.
2, filefish bait poison source Monitoring and Controlling
1. feed source is definite:
Select freezing sand lance, trash fish and the shrimp of globe fish happiness food, avoid the iced trash fish of throwing something and feeding.Refrigerating process can reduce various mushroom existence amounts in a large number, reduces the tetraodotoxin quantity that microorganism is synthesized, accumulates in the enteron aisle;
2. feed: throw in fresh bait such as freezing sand lance, trash fish, shrimp, feeding volume is thrown something and fed 4-5 time by the 20%-30% of fish body gross weight every day;
3. filefish liver, ovary severe toxicity organ toxicity monitoring:
Made regular check on liver, ovary content of toxins in every month;
4. develop total man worker's feed, solve the palatability problem, further reduce food source property toxin source.
Cultivation method of blowfish with controlled toxicity provided by the invention cuts off and avoids the source of globe fish toxin from environmental factor and food factor two aspects, make that globe fish vivotoxin content is very little, reach the standard of actual non-poisonous material, utilize this method to provide a kind of good and safe aquatic products for people live in the demand of guaranteeing to satisfy under the safe prerequisite aspects such as eater's cuisines, nutrition, disease preventing and treating.
Embodiment:
In recent years, certain aquatic products industry development company has carried out the tentative breed work of globe fish (comprising three kinds of red fin east Puffer, false fine east Puffer, yellowfin east Puffer) according to cultivation method of blowfish with controlled toxicity provided by the invention, therebetween implementation step and aforementioned method is followed in the equal strictness of Monitoring and Controlling of malicious source and specification requirement is carried out.Measure through health ministry and province's authoritative department, every gram is cultured the toxin that the filefish that grows up to only contains 5 MU (mouse dosage unit), and the toxin (toxin that is equivalent to 1 ton of globe fish) of edible 10000 MU of people's a meal could be poisoned, and the thing of at every turn being eaten got final product metabolism in 24 hours and finishes, thereby do not exist toxin to accumulate.The foodsafety toxicology actual result of being done through certain province disease prevention and control center is judged, this true border of globe fish product non-poisonous material.Practice shows, and is very little by the globe fish vivotoxin content of artificial controlled poison manization breed, add and note poisonous position taking-up in the filefish body before edible, or process technology method according to the rules is processed into manufactured goods, eats saferly, can relievedly eat fully.

Claims (2)

1. cultivation method of blowfish with controlled toxicity, it is characterized in that: this cultural method is the outdoor ponds breeding way, and implementation step is as follows:
(1), the pond is built: the pond area is 3-5km 2, the north-south is chosen in free of contamination area and has independently advances pumping equipment, more than the mean depth of water 2m;
(2), the sterilization of cleaning up the pond: clean up the pond and to carry out putting seedling first half of the month, be equipped with oxygen increasing equipment, turn over rake at the bottom of to the pond, tan by the sun mid-May, make the rancid abundant oxidation Decomposition of beach mud of the melanism of wherein depositing, simultaneously per hectare is with the quicklime 2000kg sterilization of splashing at the bottom of to the pond, drain after 3 days, airing was intake after one week, and the depth of water is more than 1.5m; Adopt winter the mode of dry freeze, weathering to make at the bottom of the pond gradually to low ammonia nitrogen index recovery, disappear, irrigation and drainage mid-March in 1 year 1-2 time, water washes away at the bottom of the pond and with Humus drains with water;
(3), seed is thrown in: select kind pure, no sick and wounded, physique good, the seed of the long 50-60mm of specification homogeneous body is thrown in, at the beginning of release time is late May to 6 month, water temperature is more than 15 ℃, injected volume be one age 600-800 tail/mu or two age 30-50 tail/mu, breeding density is 3500-4500 tail/hm2;
(4), feed: throwing in the freezing fresh bait of sand lance, shrimp, is auxilliary with artitificial food, and feeding volume is by the 20%-30% of fish body total amount, and throw something and feed 4-5 every day;
(5), breeding condition and water quality adjustment: the pond depth of water will remain on 1.2m-1.5m, and water temperature 15-30 ℃, transparency will remain on 0.3-0.4m, intensity of illumination 500lx-1000lx; Changed water once in per 15 days, quantity of exchanged water will reach 40% of total water body, and the oxygen content in the water body remains on more than the 5mg/L, and pH value remains between the 7.8-8.3, salinity 15-32, ammonia-nitrogen content≤1mg/L, nitroso nitrogen≤0.1mg/L;
(6), daily management: repeatedly patrol the pool every day, observe the active situation of water quality and fish, check that fry ingests, growth and disease, pinpoint the problems and in time handle; Carry out water quality adjustment and change the water conservancy project work; Long and the body weight of routine test seed body is checked the fry growing state, and the discovery fish disease should in time be picked out;
(7), survive the winter: when water temperature is reduced to 12 ℃, move into fry indoor or survive the winter at booth, water temperature remains on more than 12 ℃ and prevents freeze injury; The freezing new iced trash fish of throwing something and feeding every day once, a day feeding volume is the 2-3% of fish body weight; The pond fell in every month once, thoroughly clean and sterilize; Change water every day once, quantity of exchanged water is 50%;
(8), survive the winter after, water temperature rises to 15 ℃, shifts out the outdoor breeding large size fingerling again, up to marketable fish size;
(9), big specification fish culture: select the above healthy fish of body weight 150g/ tail, breeding density is 700-800 tail/hm 2, the freezing new iced trash fish of throwing something and feeding every day 2 times, a day feeding volume is the 2-3% of fish body weight.
2. cultivation method of blowfish with controlled toxicity according to claim 1 is characterized in that:
Be the key point of this method to the control in filefish growing environment poison source and the Monitoring and Controlling in bait poison source in breeding process, the main practice is:
(1), filefish growing environment poison source control
1. pond sterilization:
Thoroughly clean up the pond, eliminate benthic mollusca, algae, bacterium, actinomycetes, form before putting in a suitable place to breed in " inanimate object district " at the bottom of the pond, concrete grammar is to use machine to clean up the pond before the water inlet of pond the beginning of spring, root out mud at the bottom of the pond, throw in 1 kilogram in bleaching powder, evenly spray sterilizing for per 5 square metres; Airing was intake after one week, was equipped with in the future to culture;
2. water quality control:
I is installed apparatus for ultraviolet disinfection and ozone sterilization device at water inlet, and seawater is disinfected, and reduces the microbial poison source, increases the seawater bacterial digestion simultaneously and cultivates, and forms advantage group, thereby reduces the intoxicating micro organism quantity;
II Chi Shui was every several days suspicious bacterial species of microscopy monitoring seawater, and the pseudomonas bacterium can produce dehydration-TTX and actinomyces thereof in the filefish enteron aisle; The next biological calcareous algae of supervision filefish food chain, conch, decorative pattern are liked clean crab, the toxic biology of bluish green spotted parrot fish;
The III pond depth of water will remain on 1.2m-1.5m, and transparency will remain on more than the 0.3-0.4m; Once changed water in per 15 days, quantity of exchanged water will reach more than 40% of total water body, and the oxygen content in the water body remains on 5mg/L, and pH value remains between the 7.8-8.3;
(2), filefish bait poison source Monitoring and Controlling
1. feed source is definite:
Select freezing sand lance, trash fish and the shrimp of globe fish happiness food, avoid the iced trash fish of throwing something and feeding, refrigerating process can reduce various mushroom existence amounts in a large number, and the interior microorganism of minimizing enteron aisle is synthetic, the tetraodotoxin quantity of accumulation;
2. feed: throw in the freezing fresh bait of sand lance, trash fish, shrimp, feeding volume is by the 20%-30% of fish body gross weight, and throw something and feed 4-5 every day;
3. filefish liver, ovary severe toxicity organ toxicity monitoring;
Made regular check on liver, ovary content of toxins in every month;
4. develop total man worker's feed, solve the palatability problem, further reduce food source property toxin source.
CN2008100116623A 2008-06-04 2008-06-04 Cultivation method of blowfish with controlled toxicity Expired - Fee Related CN101300962B (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103300414A (en) * 2013-06-04 2013-09-18 江苏中洋集团股份有限公司 Preparing method of quick-freezing globefish porridge

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CN103651191B (en) * 2012-09-24 2015-03-25 上海市水产研究所 Micro-ecology regulating and controlling method of sea horse circulating water breeding system
CN106106295B (en) * 2016-07-26 2019-10-11 江苏中洋集团股份有限公司 A kind of fugu obscurus marketable fish stereo ecological cultural method
CN107821242A (en) * 2017-10-31 2018-03-23 广西沙田仙人滩农业投资有限公司 A kind of cage culture method of filefish
CN110050731A (en) * 2019-05-29 2019-07-26 江苏中洋集团股份有限公司 The high globe fish cultural method of toxin
CN113016705A (en) * 2021-03-15 2021-06-25 上海海洋大学 Control method of biological heat source in container culture tail water

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CN103300414A (en) * 2013-06-04 2013-09-18 江苏中洋集团股份有限公司 Preparing method of quick-freezing globefish porridge

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