CN107881131A - Compound bacteria, preparation method and its application in preparation sweet potato fertilizer - Google Patents
Compound bacteria, preparation method and its application in preparation sweet potato fertilizer Download PDFInfo
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- CN107881131A CN107881131A CN201711140155.5A CN201711140155A CN107881131A CN 107881131 A CN107881131 A CN 107881131A CN 201711140155 A CN201711140155 A CN 201711140155A CN 107881131 A CN107881131 A CN 107881131A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05C—NITROGENOUS FERTILISERS
- C05C9/00—Fertilisers containing urea or urea compounds
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
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Abstract
The invention discloses a kind of compound bacteria, preparation method and its application of sweet potato fertilizer is being prepared, is belonging to fertilizer preparing technical field.Described compound bacteria, in units of parts by weight, including following raw material：20 40 parts of colloid bacillus cereus, 15 30 parts of nitrobacteria, 58 parts of photosynthetic bacteria, 69 parts of rhizobium, 36 parts of phosphobacteria, 48 parts of B. mucilaginocus.The compound bacteria is through overactivation, expanded made of the steps such as culture.The compound bacteria of the present invention is applied to sweet potato can be promoted quickly to grow up healthy and sound in sweet potato fertilizer, improves sweet potato per mu yield.
Description
【Technical field】
The invention belongs to fertilizer preparing technical field, and in particular to a kind of compound bacteria, preparation method and its prepare sweet potato
The application of fertilizer.
【Background technology】
Sweet potato also known as sweet potato, pachyrhizus, sweet potato etc., rich in protein, starch, pectin, cellulose, amino acid, vitamin and
Several mineral materials, there is the reputation of " life prolonging food ".
Sweet potato contains nutritional ingredient necessary to the human bodies such as abundant starch, vitamin, cellulose, also containing abundant magnesium,
The mineral elements such as phosphorus, calcium and linoleic acid etc..These materials can keep blood vessel elasticity, extremely have to preventing and treating senility habitual constipation
Effect.Sweet potato contains a large amount of celluloses and pectin for being not easy to be inhaled zymigen destruction, energy liquid that stimulates digestion secretion and enterogastric peristalsis,
So as to play defecating feces excretion.In addition, the beta carotene of its rich content is a kind of effective antioxidant, help to remove body
Interior free radical.
Eco-fertilizer is primarily referred to as microbial manure, and it is to cause crop to obtain specific fertilizer effect with the vital movement of microorganism
A kind of product answered, it is one kind that fertilizer is used in agricultural production.It has the history of nearly 50 years in China, from rhizobium
Agent --- bacterial fertilizer --- microbial manure, from differentiation nominally it is stated that the mistake that China's microbial manure progressively develops
Journey.But for a long time, because some bacterial manure effects are unstable, bacterium number is few, invalid and harmful miscellaneous bacteria is more, bacterium in soil into
Motility rate is low, causes bacterial manure undesirable to the effect of crop yield.
A kind of Chinese patent application document " sweet potato fertilizer and preparation method thereof (publication number：CN107188750A) " disclose
A kind of sweet potato fertilizer and preparation method thereof.The sweet potato fertilizer of the invention is mainly by turf 40-50 parts, sugarcane bagasse 30-40 parts, biology
Charcoal 10-20 parts, potassium fulvate 10-20 parts, kaolin 10-15 parts, urea 5-15 parts, mannitol 1-3 parts, sodium molybdate 0.3-0.7
Part and composite bacteria 1-3 parts are passed through mixed fermentation, are made.The sweet potato fertilizer of the invention has noresidue, free of contamination spy
Point, and its preparation method is simple, easy to implement, cost is low, has preferable popularizing application prospect.It is but red there is this is applied
The problem of sweet potato per mu yield obtained after potato fertilizer is relatively low.
【The content of the invention】
The present invention provides a kind of compound bacteria, preparation method and its is preparing the application of sweet potato fertilizer, to solve in China specially
A kind of sharp application documents " sweet potato fertilizer and preparation method thereof (publication number：CN107188750A fertilizer formula disclosed in) " and method
On, how to optimize component, dosage, method, improve sweet potato per mu yield.
To solve above technical problem, the present invention uses following technical scheme：
A kind of compound bacteria, in units of parts by weight, including following raw material：Colloid bacillus cereus 20-40 parts, nitrobacteria
15-30 parts, photosynthetic bacteria 5-8 parts, rhizobium 6-9 parts, phosphobacteria 3-6 parts, B. mucilaginocus 4-8 parts.
The present invention also provides a kind of preparation method of compound bacteria, comprises the following steps：
A, respectively by colloid bacillus cereus, nitrobacteria, photosynthetic bacteria, rhizobium, phosphobacteria, B. mucilaginocus in proportion in temperature
To be activated at 23-25 DEG C, expanding culture, with standby；
B, the Bacillus after culture, nitrobacteria, saccharomycete, photosynthetic bacteria, rhizobium, phosphobacteria, potassium will be expanded to step a
Bacterium is mixed and adds biological growth stimulant, biological nutritive agent, 500-800 part water are well mixed, in the case where temperature is 24-26 DEG C
After cultivating 15-18h, water removal is centrifuged in the case where rotating speed is 2000-3000r/min, compound bacteria is made；The biological nutritive agent with
Parts by weight are unit, including following raw material：Yeast extract 52-60 parts, sucrose 45-50 parts, peptone 20-30 parts, potassium acetate 22-26
Part, sodium phosphate 12-18 parts；The biological growth stimulant is in units of parts by weight, including following raw material：Cerium chloride 50-60 parts,
Aluminum sulfate 30-40 parts.
The present invention also provide a kind of compound bacteria prepare sweet potato fertilizer application, described sweet potato fertilizer, using parts by weight as
Unit, including following raw material：Turf 55-70 parts, sugarcane bagasse 42-50 parts, poplar charcoal 24-30 parts, potassium fulvate 15-25
Part, kaolin 16-20 parts, urea 12-18 parts, mannitol 4-6 parts, sodium molybdate 0.8-1.2 parts, compound bacteria 3.5-5.5 parts, polyoxy
Change ethene 2-4 parts, brassinosteroid 0.6-0.9 parts, chitosan oligosaccharide 0.3-0.5 parts.
Preferably, the preparation method of described sweet potato fertilizer, comprises the following steps：
S1：By turf, sugarcane bagasse, poplar charcoal, potassium fulvate, kaolin, urea, mannitol, sodium molybdate, compound bacteria
Mix, fermented under ul-trasonic irradiation by weight proportion, zymotic fluid is made；
S2：Zymotic fluid made from step S1 is stood and abandons precipitation, and remaining ferment liquid is concentrated, concentrate is obtained, by institute
Concentrate drying is stated, mixture is made；
S3：Mixture made from step S2 is crushed, polyethylene glycol oxide, brassinosteroid, chitosan oligosaccharide are by weight after sifting out
Amount ratio is well mixed, and particle is made, and sweet potato fertilizer is made.
Preferably, the condition fermented described in step S1 under ul-trasonic irradiation：It is 150-200W in ultrasonic power,
Temperature is to be fermented at 50-55 DEG C, is fermented 4-6 days.
Preferably, remaining zymotic fluid concentrates at 54-58 DEG C described in step S2.
Preferably, concentrate described in step S2 is dried at 70-75 DEG C.
Preferably, the drying to water content is 3%-6%.
Preferably, sieve described in step S3 is 60-100 mesh.
Preferably, the particle diameter of particle described in step S3 is 0.1-0.3mm.
The invention has the advantages that：
(1) from embodiment 1-3 and the data of comparative example 6, using the sweet potato average yield per mu of embodiment 1-3 sweet potato fertilizer
Amount significantly improves；Simultaneously from embodiment 1-3 data, embodiment 2 is optimum embodiment.
(2) from embodiment 2 and comparative example 1-5 data, compound bacteria, polyethylene glycol oxide, brassinosteroid, chitosan oligosaccharide
Synergy is served in sweet potato fertilizer is prepared, collaboration improves sweet potato per mu yield, and this is probably：
1) fertilizer made of compound bacteria has volume increase, the function of improving quality, also substantially reduces nitrate in plant,
Nitrite and content of beary metal, improve the effect such as chemical fertilizer utilization ratio and fertilizing soil.
2) polyethylene glycol oxide is water-wet side, can be combined with fertilizer particle, and polyethylene glycol oxide has good water resistance, and its
Strand can stretch well in water, uniformly be arranged in interface, increase steric hindrance, be quickly dispersed in fertilizer particle
In water.
3) division of the brassinosteroid to cell has obvious facilitation, can play swollen megalocarpous effect；Can be saturating
The differentiation of bud is led, promotes side shoot generation, increases branch number, increase and spend number, improve becoming pregnant property of pollen, increase fruit number, improve production
Amount.
4) amino in chitosan oligosaccharide structure has the gentle control property of complexing, ion exchangeable, can be with fertilizer intermediate ion base
Group combines, and can not only be continuously plant and provide nutrient, long action time, moreover it is possible to excites defensin gene in plant, produces tool
There are chitinase, dextranase, guarantor's element and PR albumen of resistant effect etc., and with activating cell effect, contribute to aggrieved plant
Recovery, promoting root growth strong sprout, strengthen the resistance of crop, promote plant growing cycle must through the various stages, such as light and effect rank
Section etc., plant is obtained the amount of product by photosynthesis etc. in itself and maintain autologous growth enough.Chitosan oligosaccharide also have antivirus,
Bactericidal, fungicidal action, not only there is extremely strong preventing and treating and eradicant action to fungi, bacterium, virus, protect plant, make
The disease resistance of plant, the enhancing of anti-tolerance.Chitosan oligosaccharide is used cooperatively with brassinosteroid can improve plant in rape element
The absorption of ester, can promote Fruit, and and can promotes plant growth, flower and fruit protecting, prevents dehiscent fruit shedding, effectively change
The real quality of good fruit, improves yield.
【Embodiment】
For ease of more fully understanding the present invention, it is illustrated by following examples, these embodiments belong to the present invention's
Protection domain, but do not limit the scope of the invention.
The sweet potato fertilizer, in units of parts by weight, including following raw material：Turf 55-70 parts, sugarcane bagasse 42-50 parts, poplar
Wooden charcoal 24-30 parts, potassium fulvate 15-25 parts, kaolin 16-20 parts, urea 12-18 parts, mannitol 4-6 parts, sodium molybdate
0.8-1.2 parts, compound bacteria 3.5-5.5 parts, polyethylene glycol oxide 2-4 parts, brassinosteroid 0.6-0.9 parts, chitosan oligosaccharide 0.3-0.5
Part；
Described compound bacteria is in units of parts by weight, including following raw material：Colloid bacillus cereus 20-40 parts, nitrobacteria
15-30 parts, photosynthetic bacteria 5-8 parts, rhizobium 6-9 parts, phosphobacteria 3-6 parts, B. mucilaginocus 4-8 parts；
The preparation method of described sweet potato fertilizer, comprises the following steps：
S1：By turf, sugarcane bagasse, poplar charcoal, potassium fulvate, kaolin, urea, mannitol, sodium molybdate, compound bacteria
Mix by weight proportion, be 150-200W in ultrasonic power, temperature is to be fermented at 50-55 DEG C, ferments 4-6 days, is made
Zymotic fluid；
The preparation method of compound bacteria, comprises the following steps described in step S1：
A, respectively by colloid bacillus cereus, nitrobacteria, photosynthetic bacteria, rhizobium, phosphobacteria, B. mucilaginocus in proportion in temperature
To be activated at 23-25 DEG C, expanding culture, with standby；
B, the Bacillus after culture, nitrobacteria, saccharomycete, photosynthetic bacteria, rhizobium, phosphobacteria, potassium will be expanded to step a
Bacterium is mixed and adds biological growth stimulant, biological nutritive agent, 500-800 part water are well mixed, in the case where temperature is 24-26 DEG C
After cultivating 15-18h, water removal is centrifuged in the case where rotating speed is 2000-3000r/min, compound bacteria is made；The biological nutritive agent with
Parts by weight are unit, including following raw material：Yeast extract 52-60 parts, sucrose 45-50 parts, peptone 20-30 parts, potassium acetate 22-26
Part, sodium phosphate 12-18 parts；The biological growth stimulant is in units of parts by weight, including following raw material：Cerium chloride 50-60 parts,
Aluminum sulfate 30-40 parts；
S2：Zymotic fluid made from step S1 is stood and abandons precipitation, and remaining ferment liquid is concentrated at 54-58 DEG C, is obtained
Concentrate, it is 3%-6% that the concentrate is dried to water content at 70-75 DEG C, and mixture is made；
S3：Mixture made from step S2 is crushed, cross 60-100 mesh sieve after with polyethylene glycol oxide, brassinosteroid,
Chitosan oligosaccharide is well mixed by weight proportion, and the particle that particle diameter is 0.1-0.3mm is made, sweet potato fertilizer is made.
It is illustrated below by more specifically embodiment.
Embodiment 1
A kind of sweet potato fertilizer, in units of parts by weight, including following raw material：58 parts of turf, 44 parts of sugarcane bagasse, poplar biology
It is 24 parts of charcoal, 15 parts of potassium fulvate, 16 parts of kaolin, 12 parts of urea, 4 parts of mannitol, 0.8 part of sodium molybdate, 3.5 parts of compound bacteria, poly-
2 parts of ethylene oxide, 0.6 part of brassinosteroid, 0.3 part of chitosan oligosaccharide；
Described compound bacteria is in units of parts by weight, including following raw material：20 parts of colloid bacillus cereus, nitrobacteria 15
Part, 5 parts of photosynthetic bacteria, 6 parts of rhizobium, 3 parts of phosphobacteria, 4 parts of B. mucilaginocus；
The preparation method of described sweet potato fertilizer, comprises the following steps：
S1：By turf, sugarcane bagasse, poplar charcoal, potassium fulvate, kaolin, urea, mannitol, sodium molybdate, compound bacteria
Mix by weight proportion, be 150W in ultrasonic power, temperature is to be fermented at 50 DEG C, ferments 6 days, zymotic fluid is made；
The preparation method of compound bacteria, comprises the following steps described in step S1：
A, respectively by colloid bacillus cereus, nitrobacteria, photosynthetic bacteria, rhizobium, phosphobacteria, B. mucilaginocus in proportion in temperature
To be activated at 23 DEG C, expanding culture, with standby；
B, the Bacillus after culture, nitrobacteria, saccharomycete, photosynthetic bacteria, rhizobium, phosphobacteria, potassium will be expanded to step a
Bacterium is mixed and adds biological growth stimulant, biological nutritive agent, 500 parts of water are well mixed, and 18h is cultivated at being 24 DEG C in temperature
Afterwards, water removal is centrifuged in the case where rotating speed is 2000r/min, compound bacteria is made；The biological nutritive agent is in units of parts by weight, bag
Include following raw material：52 parts of yeast extract, 45 parts of sucrose, 20 parts of peptone, 22 parts of potassium acetate, 12 parts of sodium phosphate；The biological growth
Stimulant is in units of parts by weight, including following raw material：50 parts of cerium chloride, 30 parts of aluminum sulfate；
S2：Zymotic fluid made from step S1 is stood and abandons precipitation, and remaining ferment liquid is concentrated at 54 DEG C, is concentrated
Thing, it is 3% that the concentrate is dried to water content at 70 DEG C, and mixture is made；
S3：Mixture made from step S2 is crushed, it is few with polyethylene glycol oxide, brassinosteroid, shell after 60 mesh sieve excessively
Sugar is well mixed by weight proportion, and the particle that particle diameter is 0.1mm is made, sweet potato fertilizer is made.
Embodiment 2
A kind of sweet potato fertilizer, in units of parts by weight, including following raw material：63 parts of turf, 48 parts of sugarcane bagasse, poplar biology
26 parts of charcoal, 20 parts of potassium fulvate, 19 parts of kaolin, 16 parts of urea, 5 parts of mannitol, 1 part of sodium molybdate, 4.5 parts of compound bacteria, polyoxy
Change 3.2 parts of ethene, 0.8 part of brassinosteroid, 0.4 part of chitosan oligosaccharide；
Described compound bacteria is in units of parts by weight, including following raw material：30 parts of colloid bacillus cereus, nitrobacteria 25
Part, 7 parts of photosynthetic bacteria, 8 parts of rhizobium, 5 parts of phosphobacteria, 6 parts of B. mucilaginocus；
The preparation method of described sweet potato fertilizer, comprises the following steps：
S1：By turf, sugarcane bagasse, poplar charcoal, potassium fulvate, kaolin, urea, mannitol, sodium molybdate, compound bacteria
Mix by weight proportion, be 180W in ultrasonic power, temperature is to be fermented at 52 DEG C, ferments 5 days, zymotic fluid is made；
The preparation method of compound bacteria, comprises the following steps described in step S1：
A, respectively by colloid bacillus cereus, nitrobacteria, photosynthetic bacteria, rhizobium, phosphobacteria, B. mucilaginocus in proportion in temperature
To be activated at 24 DEG C, expanding culture, with standby；
B, the Bacillus after culture, nitrobacteria, saccharomycete, photosynthetic bacteria, rhizobium, phosphobacteria, potassium will be expanded to step a
Bacterium is mixed and adds biological growth stimulant, biological nutritive agent, 700 parts of water are well mixed, and 17h is cultivated at being 25 DEG C in temperature
Afterwards, water removal is centrifuged in the case where rotating speed is 2500r/min, compound bacteria is made；The biological nutritive agent is in units of parts by weight, bag
Include following raw material：56 parts of yeast extract, 48 parts of sucrose, 25 parts of peptone, 25 parts of potassium acetate, 17 parts of sodium phosphate；The biological growth
Stimulant is in units of parts by weight, including following raw material：55 parts of cerium chloride, 35 parts of aluminum sulfate；
S2：Zymotic fluid made from step S1 is stood and abandons precipitation, and remaining ferment liquid is concentrated at 57 DEG C, is concentrated
Thing, it is 5% that the concentrate is dried to water content at 72 DEG C, and mixture is made；
S3：Mixture made from step S2 is crushed, it is few with polyethylene glycol oxide, brassinosteroid, shell after 80 mesh sieve excessively
Sugar is well mixed by weight proportion, and the particle that particle diameter is 0.2mm is made, sweet potato fertilizer is made.
Embodiment 3
A kind of sweet potato fertilizer, in units of parts by weight, including following raw material：68 parts of turf, 47 parts of sugarcane bagasse, poplar biology
It is 30 parts of charcoal, 25 parts of potassium fulvate, 20 parts of kaolin, 18 parts of urea, 6 parts of mannitol, 1.2 parts of sodium molybdate, 5.5 parts of compound bacteria, poly-
4 parts of ethylene oxide, 0.9 part of brassinosteroid, 0.5 part of chitosan oligosaccharide；
Described compound bacteria is in units of parts by weight, including following raw material：40 parts of colloid bacillus cereus, nitrobacteria 30
Part, 8 parts of photosynthetic bacteria, 9 parts of rhizobium, 6 parts of phosphobacteria, 8 parts of B. mucilaginocus；
The preparation method of described sweet potato fertilizer, comprises the following steps：
S1：By turf, sugarcane bagasse, poplar charcoal, potassium fulvate, kaolin, urea, mannitol, sodium molybdate, compound bacteria
Mix by weight proportion, be 200W in ultrasonic power, temperature is to be fermented at 55 DEG C, ferments 4 days, zymotic fluid is made；
The preparation method of compound bacteria, comprises the following steps described in step S1：
A, respectively by colloid bacillus cereus, nitrobacteria, photosynthetic bacteria, rhizobium, phosphobacteria, B. mucilaginocus in proportion in temperature
To be activated at 25 DEG C, expanding culture, with standby；
B, the Bacillus after culture, nitrobacteria, saccharomycete, photosynthetic bacteria, rhizobium, phosphobacteria, potassium will be expanded to step a
Bacterium is mixed and adds biological growth stimulant, biological nutritive agent, 800 parts of water are well mixed, and 15h is cultivated at being 26 DEG C in temperature
Afterwards, water removal is centrifuged in the case where rotating speed is 3000r/min, compound bacteria is made；The biological nutritive agent is in units of parts by weight, bag
Include following raw material：60 parts of yeast extract, 50 parts of sucrose, 30 parts of peptone, 26 parts of potassium acetate, 18 parts of sodium phosphate；The biological growth
Stimulant is in units of parts by weight, including following raw material：60 parts of cerium chloride, 40 parts of aluminum sulfate；
S2：Zymotic fluid made from step S1 is stood and abandons precipitation, and remaining ferment liquid is concentrated at 58 DEG C, is concentrated
Thing, it is 3% that the concentrate is dried to water content at 75 DEG C, and mixture is made；
S3：Mixture made from step S2 is crushed, it is few with polyethylene glycol oxide, brassinosteroid, shell after 100 mesh sieve excessively
Sugar is well mixed by weight proportion, and the particle that particle diameter is 0.3mm is made, sweet potato fertilizer is made.
Comparative example 1
It is essentially identical with the preparation technology of embodiment 2, have only the difference is that lacking in the raw material of preparation sweet potato fertilizer compound
Bacterium, polyethylene glycol oxide, brassinosteroid, chitosan oligosaccharide.
Comparative example 2
It is essentially identical with the preparation technology of embodiment 2, have only the difference is that lacking in the raw material of preparation sweet potato fertilizer compound
Bacterium.
Comparative example 3
It is essentially identical with the preparation technology of embodiment 2, have only the difference is that lacking polyoxy in the raw material of preparation sweet potato fertilizer
Change ethene.
Comparative example 4
It is essentially identical with the preparation technology of embodiment 2, have only the difference is that lacking rape in the raw material of preparation sweet potato fertilizer
Plain lactone.
Comparative example 5
It is essentially identical with the preparation technology of embodiment 2, have only the difference is that lacking shell widow in the raw material of preparation sweet potato fertilizer
Sugar.
Comparative example 6
Using a kind of Chinese patent application document " sweet potato fertilizer and preparation method thereof (publication number：CN107188750A it is) " real
The technique for applying a 1-3 prepares sweet potato fertilizer.
With identical method, the sweet potato of same breed is planted in Guangxi town, respectively using embodiment 1-3 and comparative example 1-
Fertilizer in 6, per 1m20.5 kilogram of fertilizer is respectively applied in soil, after sweet potato maturation, detects per mu yield, testing result such as following table
It is shown.
| Test group | Per mu yield (kg) |
| Embodiment 1 | 2843.15 |
| Embodiment 2 | 2901.42 |
| Embodiment 3 | 2816.79 |
| Comparative example 1 | 2314.56 |
| Comparative example 2 | 2784.97 |
| Comparative example 3 | 2812.67 |
| Comparative example 4 | 2847.16 |
| Comparative example 5 | 2821.54 |
| Comparative example 6 | 2016.42-2201.43 |
As seen from the above table：(1) from embodiment 1-3 and the data of comparative example 6, using embodiment 1-3 sweet potato fertilizer
Sweet potato per mu yield significantly improves；Simultaneously from embodiment 1-3 data, embodiment 2 is optimum embodiment.
(2) from embodiment 2 and comparative example 1-5 data, compound bacteria, polyethylene glycol oxide, brassinosteroid, chitosan oligosaccharide
Synergy is served in sweet potato fertilizer is prepared, collaboration improves sweet potato per mu yield, and this is probably：
1) fertilizer made of compound bacteria has volume increase, the function of improving quality, also substantially reduces nitrate in plant,
Nitrite and content of beary metal, improve the effect such as chemical fertilizer utilization ratio and fertilizing soil.
2) polyethylene glycol oxide is water-wet side, can be combined with fertilizer particle, and polyethylene glycol oxide has good water resistance, and its
Strand can stretch well in water, uniformly be arranged in interface, increase steric hindrance, be quickly dispersed in fertilizer particle
In water.
3) division of the brassinosteroid to cell has obvious facilitation, can play swollen megalocarpous effect；Can be saturating
The differentiation of bud is led, promotes side shoot generation, increases branch number, increase and spend number, improve becoming pregnant property of pollen, increase fruit number, improve production
Amount.
4) amino in chitosan oligosaccharide structure has the gentle control property of complexing, ion exchangeable, can be with fertilizer intermediate ion base
Group combines, and can not only be continuously plant and provide nutrient, long action time, moreover it is possible to excites defensin gene in plant, produces tool
There are chitinase, dextranase, guarantor's element and PR albumen of resistant effect etc., and with activating cell effect, contribute to aggrieved plant
Recovery, promoting root growth strong sprout, strengthen the resistance of crop, promote plant growing cycle must through the various stages, such as light and effect rank
Section etc., plant is obtained the amount of product by photosynthesis etc. in itself and maintain autologous growth enough.Chitosan oligosaccharide also have antivirus,
Bactericidal, fungicidal action, not only there is extremely strong preventing and treating and eradicant action to fungi, bacterium, virus, protect plant, make
The disease resistance of plant, the enhancing of anti-tolerance.Chitosan oligosaccharide is used cooperatively with brassinosteroid can improve plant in rape element
The absorption of ester, can promote Fruit, and and can promotes plant growth, flower and fruit protecting, prevents dehiscent fruit shedding, effectively change
The real quality of good fruit, improves yield.
Above content is it cannot be assumed that present invention specific implementation is confined to these explanations, for the technical field of the invention
Those of ordinary skill for, under the premise of present inventive concept is not departed from, some simple deduction or replace can also be made, all should
When being considered as belonging to the scope of patent protection that is determined by the claims submitted of the present invention.
Claims (10)
- A kind of 1. compound bacteria, it is characterised in that in units of parts by weight, including following raw material：Colloid bacillus cereus 20-40 parts, Nitrobacteria 15-30 parts, photosynthetic bacteria 5-8 parts, rhizobium 6-9 parts, phosphobacteria 3-6 parts, B. mucilaginocus 4-8 parts.
- 2. a kind of preparation method of compound bacteria according to claim 1, it is characterised in that comprise the following steps：A, it is in temperature in proportion by colloid bacillus cereus, nitrobacteria, photosynthetic bacteria, rhizobium, phosphobacteria, B. mucilaginocus respectively Activated at 23-25 DEG C, expand culture, with standby；B, the Bacillus after culture, nitrobacteria, saccharomycete, photosynthetic bacteria, rhizobium, phosphobacteria, B. mucilaginocus will be expanded to step a Mix and add biological growth stimulant, biological nutritive agent, 500-800 part water are well mixed, temperature be 24-26 DEG C at cultivate After 15-18h, water removal is centrifuged in the case where rotating speed is 2000-3000r/min, compound bacteria is made；The biological nutritive agent is with weight Part it is unit, including following raw material：Yeast extract 52-60 parts, sucrose 45-50 parts, peptone 20-30 parts, potassium acetate 22-26 parts, Sodium phosphate 12-18 parts；The biological growth stimulant is in units of parts by weight, including following raw material：Cerium chloride 50-60 parts, sulphur Sour aluminium 30-40 parts.
- 3. a kind of compound bacteria according to claim 1 is preparing the application of sweet potato fertilizer, it is characterised in that described sweet potato Fertilizer, in units of parts by weight, including following raw material：Turf 55-70 parts, sugarcane bagasse 42-50 parts, poplar charcoal 24-30 parts, Potassium fulvate 15-25 parts, kaolin 16-20 parts, urea 12-18 parts, mannitol 4-6 parts, sodium molybdate 0.8-1.2 parts, compound bacteria 3.5-5.5 parts, polyethylene glycol oxide 2-4 parts, brassinosteroid 0.6-0.9 parts, chitosan oligosaccharide 0.3-0.5 parts.
- 4. compound bacteria according to claim 3 is preparing the application of sweet potato fertilizer, it is characterised in that described sweet potato fertilizer Preparation method, comprise the following steps：S1：By turf, sugarcane bagasse, poplar charcoal, potassium fulvate, kaolin, urea, mannitol, sodium molybdate, compound bacteria by weight Amount ratio mixes, and is fermented under ul-trasonic irradiation, and zymotic fluid is made；S2：Zymotic fluid made from step S1 is stood and abandons precipitation, and remaining ferment liquid is concentrated, obtains concentrate, will be described dense Contracting thing is dried, and mixture is made；S3：Mixture made from step S2 is crushed, polyethylene glycol oxide, brassinosteroid, chitosan oligosaccharide be by weight after sifting out Example is well mixed, and particle is made, and sweet potato fertilizer is made.
- 5. compound bacteria according to claim 3 is preparing the application of sweet potato fertilizer, it is characterised in that surpasses described in step S1 The condition fermented under sound wave effect：It is 150-200W in ultrasonic power, temperature is to be fermented at 50-55 DEG C, is fermented 4-6 days.
- 6. compound bacteria according to claim 3 is preparing the application of sweet potato fertilizer, it is characterised in that remaining described in step S2 Zymotic fluid concentrates at 54-58 DEG C.
- 7. compound bacteria according to claim 3 is preparing the application of sweet potato fertilizer, it is characterised in that dense described in step S2 Contracting thing is dried at 70-75 DEG C.
- 8. compound bacteria according to claim 7 is preparing the application of sweet potato fertilizer, it is characterised in that the drying is to aqueous Measure as 3%-6%.
- 9. compound bacteria according to claim 3 is preparing the application of sweet potato fertilizer, it is characterised in that is sieved described in step S3 Son is 60-100 mesh.
- 10. compound bacteria according to claim 3 is preparing the application of sweet potato fertilizer, it is characterised in that described in step S3 The particle diameter of particle is 0.1-0.3mm.
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| CN116004415A (en) * | 2022-05-27 | 2023-04-25 | 黄冈师范学院 | Composite microbial inoculum, biomass bacterial fertilizer special for sweet potatoes and preparation method of biomass bacterial fertilizer |
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