CN107805618B - Microbial agent for modifying saline-alkali soil tillage property and preparation method thereof - Google Patents

Microbial agent for modifying saline-alkali soil tillage property and preparation method thereof Download PDF

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CN107805618B
CN107805618B CN201711255889.8A CN201711255889A CN107805618B CN 107805618 B CN107805618 B CN 107805618B CN 201711255889 A CN201711255889 A CN 201711255889A CN 107805618 B CN107805618 B CN 107805618B
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CN107805618A (en
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李峰
蒋洁
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Abstract

The invention provides a microbial preparation for modifying the tillage property of saline-alkali soil, which is a mixture formed by culturing thiobacillus thiooxidans, streptomyces cellulosae, klebsiella oxytoca and rhodotorula glutinis and then adding potassium fulvate and sulfur in proportion, wherein the mixture is used for modifying the saline-alkali soil, can buffer and intelligently adjust the pH value of the soil, reconstruct a soil microecological community, improve the micro-environments of moisture, nutrients, and the like around the root system of a crop, and improve the stress resistance of the crop, so that the modified saline-alkali soil can be suitable for planting various crops; meanwhile, the invention also provides a preparation method of the microbial preparation for improving the tillage property of the saline-alkali soil.

Description

Microbial agent for modifying saline-alkali soil tillage property and preparation method thereof
Technical Field
The invention relates to a microbial agent for improving the plowing property of saline-alkali soil, belonging to a new soil remediation technology.
Background
The main current methods for improving saline-alkali soil are hydraulic saline-alkali, buried pipe salt elimination, plant planting and the like, but the methods need a large amount of water resources and invest a large amount of engineering, and the improvement period is long. Most cases of the prior improvement are changed into greening by improving the saline-alkali soil, plant varieties for greening are special improved varieties, the selectivity is low, the economy is not strong, and common crops cannot be planted, so the economic enthusiasm of people for saline-alkali soil improvement is restricted, the conventional indexes (pH value and salinity) of the saline-alkali soil can be improved only by a conventional improvement method, but the tiltability can not meet the requirement of planting common agricultural products, so that the comprehensive performance of soil is required to be improved when the saline-alkali soil is required to be improved to meet the requirement of planting the crops, and the tiltability of the saline-alkali soil is improved. The invention comprehensively improves the saline-alkali soil from the aspects of soil saline-alkali indexes, soil microbial activity, soil organic matters and the like, leads the soil to reach the degree of improving crops planted in the same year, has less economic investment and convenient and simple use, and is worthy of large-area popularization.
Disclosure of Invention
The technical problem to be solved by the invention is to provide a microbial preparation for modifying the tillage property of saline-alkali soil, which is a mixture formed by culturing thiobacillus thiooxidans, cellulose streptomyces, Klebsiella oxytoca and Rhodotorula glutinis and then adding potassium fulvate and sulfur in proportion. The mixture is used for saline-alkali soil transformation, can intelligently adjust the pH value of soil through buffering, reconstructs a soil micro-ecological community, improves the micro-environments of moisture, nutrients and the like around the root system of crops, improves the stress resistance of the crops, and can be suitable for planting various crops.
In order to solve the technical problems, the invention adopts the following technical scheme:
a microbial preparation for modifying the tillage property of saline-alkali soil comprises the following raw materials in parts by weight: 300 kg of solid fermented thiobacillus thiooxidans powder, 100 kg of solid fermented streptomyces cellulosae powder, 100 kg of solid fermented Klebsiella oxytoca powder, 100 kg of solid fermented Rhodotorula glutinis powder, 200 kg of potassium fulvate and 200 kg of sulfur.
The invention aims to solve another technical problem of providing a preparation method of a microbial preparation for modifying the plowing property of saline-alkali soil.
In order to solve the technical problem, the technical scheme adopted by the invention comprises the following steps:
1) inoculating thiobacillus thiooxidans to ferment by taking 30 wt% of corn straw powder, 65 wt% of humus and 5 wt% of sulfur as raw materials to prepare thiobacillus thiooxidans powder; inoculating cellulose streptomyces to ferment by taking 80% of corn straw powder, 10% of soybean meal and 10% of humus as raw materials in weight ratio to prepare cellulose streptomyces powder; inoculating klebsiella oxytoca to raw materials of 80% bran powder, 5% soybean meal powder and 15% clay in weight ratio, and fermenting to prepare klebsiella oxytoca powder; the rhodotorula glutinis powder is prepared by inoculating rhodotorula glutinis to raw materials of corn flour 70%, soybean meal 15% and bran powder 15% according to the weight ratio for fermentation.
2) Weighing 300 kg of thiobacillus thiooxidans powder prepared in the step 1), 100 kg of streptomyces cellulosae powder, 100 kg of klebsiella oxytoca powder and 100 kg of rhodotorula glutinis powder, and then weighing 200 kg of potassium fulvate and 200 kg of sulfur.
3) Uniformly mixing the materials weighed in the step 2), sealing and subpackaging to obtain the microbial preparation for modifying the tillage property of the saline-alkali soil.
The invention has the advantages that: through the metabolism of the thiobacillus thiooxidans, according to the change condition of the pH value of the soil, the sulfur is oxidized into sulfuric acid by the buffered intelligent utilization, the pH value of the soil is adjusted, and the pH value of the soil is not severely fluctuated. Cellulose streptomyces, acid-producing Klebsiella and Rhodotorula glutinis, aerobic and anaerobic bacteria, fungi, bacteria and actinomycetes cooperate with each other to regulate microbial communities in soil, accelerate soil curing and organic matter conversion capability, and improve soil aggregate structure. The microbial diversity of the soil is activated, the acid-producing klebsiella oxytoca can produce secreted mucus similar to a snail, the water and fertilizer retention of the soil is improved, and the stress resistance of crops is improved; through the matching, the sulfuric acid converted by sulfur stabilizes the pH value of the soil, improves the physical and chemical properties of the soil according to the index of the salinity and alkalinity, improves the growth performance of crops through the synergistic effect of microorganisms, and can fundamentally improve the utilization efficiency of the saline-alkali soil.
Detailed Description
The present invention will be described in further detail with reference to specific embodiments for further understanding of the features and technical means of the invention and the functions achieved.
Examples
A microbial preparation for modifying the tillage property of saline-alkali soil comprises the following raw materials in parts by weight: 300 kg of solid fermented thiobacillus thiooxidans powder, 100 kg of solid fermented streptomyces cellulosae powder, 100 kg of solid fermented Klebsiella oxytoca powder, 100 kg of solid fermented Rhodotorula glutinis powder, 200 kg of potassium fulvate and 200 kg of sulfur.
The invention aims to solve another technical problem of providing a preparation method of a microbial preparation for modifying the plowing property of saline-alkali soil.
In order to solve the technical problem, the technical scheme adopted by the invention comprises the following steps:
1) inoculating thiobacillus thiooxidans to ferment by taking 30 wt% of corn straw powder, 65 wt% of humus and 5 wt% of sulfur as raw materials to prepare thiobacillus thiooxidans powder; inoculating cellulose streptomyces to ferment by taking 80% of corn straw powder, 10% of soybean meal and 10% of humus as raw materials in weight ratio to prepare cellulose streptomyces powder; inoculating klebsiella oxytoca to raw materials of 80% bran powder, 5% soybean meal powder and 15% clay in weight ratio, and fermenting to prepare klebsiella oxytoca powder; the rhodotorula glutinis powder is prepared by inoculating rhodotorula glutinis to raw materials of corn flour 70%, soybean meal 15% and bran powder 15% according to the weight ratio for fermentation.
2) Weighing 300 kg of thiobacillus thiooxidans powder prepared in the step 1), 100 kg of streptomyces cellulosae powder, 100 kg of klebsiella oxytoca powder and 100 kg of rhodotorula glutinis powder, and then weighing 200 kg of potassium fulvate and 200 kg of sulfur.
3) Uniformly mixing the materials weighed in the step 2), sealing and subpackaging to obtain the microbial preparation for modifying the tillage property of the saline-alkali soil.
Experimental example:
subject: control tests for improving the application of the saline-alkali soil are respectively carried out on the potato planted in the saline-alkali soil of the inner Mongolia Bayankee city and the tomato planted in the saline-alkali soil of the Xinjiang Tuyankee city.
The experimental method comprises the following steps: the method comprises the steps of adopting an experimental group and a blank control group, wherein the experimental group uses a microbial agent for improving the plowing property of the saline-alkali soil, the control group uses a mixture of humic acid and sulfur with equal dosage, and index change of the saline-alkali soil, the death rate of seedlings and the yield of potatoes and tomatoes are recorded.
And (3) recording in a table:
Figure DEST_PATH_IMAGE001
the experimental results are as follows:
the experimental result shows that the PH value of the contrast soil index without the microbial agent for improving the tillage property of the saline-alkali soil is far from the ideal of an experimental group and is not suitable for farming economic crops, so that the survival rate of seedlings is quite low, and the yield and the economic benefit are directly influenced. The plot of the microbial agent for improving the plowing property of the saline-alkali soil can achieve the seedling survival rate and the yield of a fertile field, the PH value of the soil can be maintained at a neutral level, and the plot is very suitable for plowing various economic crops.
The present invention has been described in detail, and it should be understood that the detailed description and specific examples, while indicating the preferred embodiment of the invention, are intended for purposes of illustration only and are not intended to limit the scope of the invention.

Claims (1)

1. The microbial preparation for improving the tillage property of the saline-alkali soil is characterized by comprising the following raw materials in parts by weight: 300 kg of solid fermented thiobacillus thiooxidans powder, 100 kg of solid fermented streptomyces cellulosae powder, 100 kg of solid fermented Klebsiella oxytoca powder, 100 kg of solid fermented Rhodotorula glutinis powder, 200 kg of potassium fulvate and 200 kg of sulfur; the preparation method of the microbial preparation for improving the tillage property of the saline-alkali soil comprises the following steps:
1) inoculating thiobacillus thiooxidans to ferment by taking 30 wt% of corn straw powder, 65 wt% of humus and 5 wt% of sulfur as raw materials to prepare thiobacillus thiooxidans powder; inoculating cellulose streptomyces to ferment by taking 80% of corn straw powder, 10% of soybean meal and 10% of humus as raw materials in weight ratio to prepare cellulose streptomyces powder; inoculating klebsiella oxytoca to raw materials of 80% bran powder, 5% soybean meal powder and 15% clay in weight ratio, and fermenting to prepare klebsiella oxytoca powder; inoculating rhodotorula glutinis to ferment by taking 70 percent of corn flour, 15 percent of soybean meal and 15 percent of bran powder in weight ratio as raw materials to prepare rhodotorula glutinis powder;
2) weighing 300 kg of thiobacillus thiooxidans powder prepared in the step 1), 100 kg of streptomyces cellulosae powder, 100 kg of klebsiella oxytoca powder and 100 kg of rhodotorula glutinis powder, and then weighing 200 kg of potassium fulvate and 200 kg of sulfur;
3) uniformly mixing the materials weighed in the step 2), sealing and subpackaging to obtain the microbial preparation for modifying the tillage property of the saline-alkali soil.
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Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2158901A2 (en) * 2003-07-03 2010-03-03 Gilead Sciences, Inc. Alpha aztreonam lysinate
CN101675069A (en) * 2004-07-02 2010-03-17 梅坦诺米克斯有限公司 Process for the production of fine chemicals
CN105439779A (en) * 2016-01-29 2016-03-30 山东和田旺生物科技有限公司 Alga bio-fertilizer and preparation method thereof
CN105969398A (en) * 2016-05-31 2016-09-28 山东胜伟园林科技有限公司 Method for greening original soil of slight alkaline land through water-retaining agent containing cellulose degradation bacteria

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2158901A2 (en) * 2003-07-03 2010-03-03 Gilead Sciences, Inc. Alpha aztreonam lysinate
CN101675069A (en) * 2004-07-02 2010-03-17 梅坦诺米克斯有限公司 Process for the production of fine chemicals
CN105439779A (en) * 2016-01-29 2016-03-30 山东和田旺生物科技有限公司 Alga bio-fertilizer and preparation method thereof
CN105969398A (en) * 2016-05-31 2016-09-28 山东胜伟园林科技有限公司 Method for greening original soil of slight alkaline land through water-retaining agent containing cellulose degradation bacteria

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
利用硫氧化细菌改良盐碱土;张静等;《吉林大学学报(地球科学版),》;20091031;第147-151页 *
解盐促生菌在棉花根部定殖的扫描电镜观察;岳海涛等;《石河子大学学报(自然科学版)》;20070831;第416-420页 *

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