CN107751856A - 一种牡蛎保健食品及其制备方法 - Google Patents
一种牡蛎保健食品及其制备方法 Download PDFInfo
- Publication number
- CN107751856A CN107751856A CN201710960244.8A CN201710960244A CN107751856A CN 107751856 A CN107751856 A CN 107751856A CN 201710960244 A CN201710960244 A CN 201710960244A CN 107751856 A CN107751856 A CN 107751856A
- Authority
- CN
- China
- Prior art keywords
- parts
- oyster
- health food
- preparation
- fruit
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 241000237502 Ostreidae Species 0.000 title claims abstract description 64
- 235000020636 oyster Nutrition 0.000 title claims abstract description 64
- 235000013402 health food Nutrition 0.000 title claims abstract description 32
- 238000002360 preparation method Methods 0.000 title claims abstract description 26
- 235000013399 edible fruits Nutrition 0.000 claims abstract description 30
- 244000163122 Curcuma domestica Species 0.000 claims abstract description 17
- 235000003392 Curcuma domestica Nutrition 0.000 claims abstract description 17
- 235000002722 Dioscorea batatas Nutrition 0.000 claims abstract description 17
- 235000006536 Dioscorea esculenta Nutrition 0.000 claims abstract description 17
- 240000001811 Dioscorea oppositifolia Species 0.000 claims abstract description 17
- 235000003416 Dioscorea oppositifolia Nutrition 0.000 claims abstract description 17
- 244000046052 Phaseolus vulgaris Species 0.000 claims abstract description 17
- 235000010627 Phaseolus vulgaris Nutrition 0.000 claims abstract description 17
- 240000004713 Pisum sativum Species 0.000 claims abstract description 17
- 235000010582 Pisum sativum Nutrition 0.000 claims abstract description 17
- 240000001341 Reynoutria japonica Species 0.000 claims abstract description 17
- 235000018167 Reynoutria japonica Nutrition 0.000 claims abstract description 17
- 125000003118 aryl group Chemical group 0.000 claims abstract description 17
- 235000003373 curcuma longa Nutrition 0.000 claims abstract description 17
- 235000013976 turmeric Nutrition 0.000 claims abstract description 17
- 244000241872 Lycium chinense Species 0.000 claims abstract description 16
- 235000015468 Lycium chinense Nutrition 0.000 claims abstract description 16
- 235000011293 Brassica napus Nutrition 0.000 claims abstract description 15
- 235000000540 Brassica rapa subsp rapa Nutrition 0.000 claims abstract description 15
- 240000006079 Schisandra chinensis Species 0.000 claims abstract description 14
- 239000000284 extract Substances 0.000 claims abstract description 12
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 53
- 239000012676 herbal extract Substances 0.000 claims description 19
- 239000013049 sediment Substances 0.000 claims description 19
- 238000007664 blowing Methods 0.000 claims description 16
- 238000000605 extraction Methods 0.000 claims description 15
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 claims description 14
- 240000008100 Brassica rapa Species 0.000 claims description 14
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 14
- 239000007788 liquid Substances 0.000 claims description 13
- 239000000047 product Substances 0.000 claims description 13
- 239000006228 supernatant Substances 0.000 claims description 12
- 238000001556 precipitation Methods 0.000 claims description 11
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 claims description 8
- 238000010992 reflux Methods 0.000 claims description 8
- 239000000203 mixture Substances 0.000 claims description 7
- LRHPLDYGYMQRHN-UHFFFAOYSA-N N-Butanol Chemical compound CCCCO LRHPLDYGYMQRHN-UHFFFAOYSA-N 0.000 claims description 6
- 239000000463 material Substances 0.000 claims description 6
- 238000000926 separation method Methods 0.000 claims description 6
- 238000003756 stirring Methods 0.000 claims description 6
- 238000003801 milling Methods 0.000 claims description 5
- 239000003960 organic solvent Substances 0.000 claims description 4
- 238000005292 vacuum distillation Methods 0.000 claims description 3
- 238000003809 water extraction Methods 0.000 claims description 2
- 230000000694 effects Effects 0.000 abstract description 19
- 230000002929 anti-fatigue Effects 0.000 abstract description 12
- 230000036039 immunity Effects 0.000 abstract description 12
- 230000003796 beauty Effects 0.000 abstract description 5
- 239000004480 active ingredient Substances 0.000 abstract description 2
- 240000002791 Brassica napus Species 0.000 abstract 1
- 239000008280 blood Substances 0.000 description 16
- 210000004369 blood Anatomy 0.000 description 16
- 229920002527 Glycogen Polymers 0.000 description 13
- 229940096919 glycogen Drugs 0.000 description 13
- 208000003351 Melanosis Diseases 0.000 description 10
- 230000000052 comparative effect Effects 0.000 description 10
- 230000006870 function Effects 0.000 description 10
- 206010008570 Chloasma Diseases 0.000 description 8
- 239000003814 drug Substances 0.000 description 7
- 210000004185 liver Anatomy 0.000 description 7
- 241000699666 Mus <mouse, genus> Species 0.000 description 6
- 229940079593 drug Drugs 0.000 description 5
- 150000004676 glycans Chemical class 0.000 description 5
- 239000004615 ingredient Substances 0.000 description 5
- 229920001282 polysaccharide Polymers 0.000 description 5
- 239000005017 polysaccharide Substances 0.000 description 5
- 210000000952 spleen Anatomy 0.000 description 5
- 210000002784 stomach Anatomy 0.000 description 5
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 4
- 235000013305 food Nutrition 0.000 description 4
- 238000003304 gavage Methods 0.000 description 4
- 210000003734 kidney Anatomy 0.000 description 4
- 230000004060 metabolic process Effects 0.000 description 4
- 210000005036 nerve Anatomy 0.000 description 4
- 235000016709 nutrition Nutrition 0.000 description 4
- 229910052760 oxygen Inorganic materials 0.000 description 4
- 239000001301 oxygen Substances 0.000 description 4
- 230000009182 swimming Effects 0.000 description 4
- 235000019640 taste Nutrition 0.000 description 4
- 241001465754 Metazoa Species 0.000 description 3
- 235000009508 confectionery Nutrition 0.000 description 3
- 230000002496 gastric effect Effects 0.000 description 3
- 230000036541 health Effects 0.000 description 3
- 238000001802 infusion Methods 0.000 description 3
- 150000002632 lipids Chemical class 0.000 description 3
- 239000006210 lotion Substances 0.000 description 3
- 210000000056 organ Anatomy 0.000 description 3
- 230000001151 other effect Effects 0.000 description 3
- 239000002904 solvent Substances 0.000 description 3
- 238000005728 strengthening Methods 0.000 description 3
- GVJHHUAWPYXKBD-UHFFFAOYSA-N (±)-α-Tocopherol Chemical compound OC1=C(C)C(C)=C2OC(CCCC(C)CCCC(C)CCCC(C)C)(C)CCC2=C1C GVJHHUAWPYXKBD-UHFFFAOYSA-N 0.000 description 2
- FPIPGXGPPPQFEQ-UHFFFAOYSA-N 13-cis retinol Natural products OCC=C(C)C=CC=C(C)C=CC1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-UHFFFAOYSA-N 0.000 description 2
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 2
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 2
- 208000002193 Pain Diseases 0.000 description 2
- FPIPGXGPPPQFEQ-BOOMUCAASA-N Vitamin A Natural products OC/C=C(/C)\C=C\C=C(\C)/C=C/C1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-BOOMUCAASA-N 0.000 description 2
- 230000009471 action Effects 0.000 description 2
- FPIPGXGPPPQFEQ-OVSJKPMPSA-N all-trans-retinol Chemical compound OC\C=C(/C)\C=C\C=C(/C)\C=C\C1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-OVSJKPMPSA-N 0.000 description 2
- 230000002457 bidirectional effect Effects 0.000 description 2
- 230000033228 biological regulation Effects 0.000 description 2
- 229910052791 calcium Inorganic materials 0.000 description 2
- 239000011575 calcium Substances 0.000 description 2
- 235000001465 calcium Nutrition 0.000 description 2
- 230000007812 deficiency Effects 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 230000002124 endocrine Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 230000002708 enhancing effect Effects 0.000 description 2
- OVBPIULPVIDEAO-LBPRGKRZSA-N folic acid Chemical compound C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-LBPRGKRZSA-N 0.000 description 2
- 230000011132 hemopoiesis Effects 0.000 description 2
- 230000036737 immune function Effects 0.000 description 2
- 208000037805 labour Diseases 0.000 description 2
- 210000004072 lung Anatomy 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 210000003205 muscle Anatomy 0.000 description 2
- 230000001737 promoting effect Effects 0.000 description 2
- 230000008929 regeneration Effects 0.000 description 2
- 238000011069 regeneration method Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 239000013589 supplement Substances 0.000 description 2
- XOAAWQZATWQOTB-UHFFFAOYSA-N taurine Chemical compound NCCS(O)(=O)=O XOAAWQZATWQOTB-UHFFFAOYSA-N 0.000 description 2
- 229930003231 vitamin Natural products 0.000 description 2
- 235000013343 vitamin Nutrition 0.000 description 2
- 239000011782 vitamin Substances 0.000 description 2
- 229940088594 vitamin Drugs 0.000 description 2
- 235000019155 vitamin A Nutrition 0.000 description 2
- 239000011719 vitamin A Substances 0.000 description 2
- 229940045997 vitamin a Drugs 0.000 description 2
- 150000003722 vitamin derivatives Chemical class 0.000 description 2
- 206010002660 Anoxia Diseases 0.000 description 1
- 241000976983 Anoxia Species 0.000 description 1
- 201000001320 Atherosclerosis Diseases 0.000 description 1
- 206010010071 Coma Diseases 0.000 description 1
- 206010010075 Coma hepatic Diseases 0.000 description 1
- 206010010774 Constipation Diseases 0.000 description 1
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 1
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 description 1
- 206010012289 Dementia Diseases 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 206010021143 Hypoxia Diseases 0.000 description 1
- 206010023126 Jaundice Diseases 0.000 description 1
- 206010067125 Liver injury Diseases 0.000 description 1
- 244000241838 Lycium barbarum Species 0.000 description 1
- 235000015459 Lycium barbarum Nutrition 0.000 description 1
- 241000237852 Mollusca Species 0.000 description 1
- 241000699670 Mus sp. Species 0.000 description 1
- OVBPIULPVIDEAO-UHFFFAOYSA-N N-Pteroyl-L-glutaminsaeure Natural products C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)NC(CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-UHFFFAOYSA-N 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- 208000013738 Sleep Initiation and Maintenance disease Diseases 0.000 description 1
- 229930182558 Sterol Natural products 0.000 description 1
- 102000019197 Superoxide Dismutase Human genes 0.000 description 1
- 108010012715 Superoxide dismutase Proteins 0.000 description 1
- 206010044565 Tremor Diseases 0.000 description 1
- 229930003268 Vitamin C Natural products 0.000 description 1
- 229930003427 Vitamin E Natural products 0.000 description 1
- 229930003448 Vitamin K Natural products 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 206010048232 Yawning Diseases 0.000 description 1
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 1
- 210000001015 abdomen Anatomy 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000003044 adaptive effect Effects 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- OFCNXPDARWKPPY-UHFFFAOYSA-N allopurinol Chemical compound OC1=NC=NC2=C1C=NN2 OFCNXPDARWKPPY-UHFFFAOYSA-N 0.000 description 1
- 235000001014 amino acid Nutrition 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 208000007502 anemia Diseases 0.000 description 1
- 230000007953 anoxia Effects 0.000 description 1
- 230000003712 anti-aging effect Effects 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 230000002785 anti-thrombosis Effects 0.000 description 1
- 230000000259 anti-tumor effect Effects 0.000 description 1
- 239000003146 anticoagulant agent Substances 0.000 description 1
- 230000036528 appetite Effects 0.000 description 1
- 235000019789 appetite Nutrition 0.000 description 1
- 229910052788 barium Inorganic materials 0.000 description 1
- DSAJWYNOEDNPEQ-UHFFFAOYSA-N barium atom Chemical compound [Ba] DSAJWYNOEDNPEQ-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000017531 blood circulation Effects 0.000 description 1
- 230000036772 blood pressure Effects 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- 230000037396 body weight Effects 0.000 description 1
- 210000000988 bone and bone Anatomy 0.000 description 1
- 210000001185 bone marrow Anatomy 0.000 description 1
- 230000011712 cell development Effects 0.000 description 1
- 230000004663 cell proliferation Effects 0.000 description 1
- 230000007969 cellular immunity Effects 0.000 description 1
- 210000003169 central nervous system Anatomy 0.000 description 1
- 230000002490 cerebral effect Effects 0.000 description 1
- 230000004087 circulation Effects 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 229910052802 copper Inorganic materials 0.000 description 1
- 239000010949 copper Substances 0.000 description 1
- 230000034994 death Effects 0.000 description 1
- 230000006837 decompression Effects 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
- 238000004332 deodorization Methods 0.000 description 1
- 230000001066 destructive effect Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 230000009977 dual effect Effects 0.000 description 1
- 239000000686 essence Substances 0.000 description 1
- 239000003925 fat Substances 0.000 description 1
- 235000019197 fats Nutrition 0.000 description 1
- 229930003935 flavonoid Natural products 0.000 description 1
- 150000002215 flavonoids Chemical class 0.000 description 1
- 235000017173 flavonoids Nutrition 0.000 description 1
- 229960000304 folic acid Drugs 0.000 description 1
- 235000019152 folic acid Nutrition 0.000 description 1
- 239000011724 folic acid Substances 0.000 description 1
- WIGCFUFOHFEKBI-UHFFFAOYSA-N gamma-tocopherol Natural products CC(C)CCCC(C)CCCC(C)CCCC1CCC2C(C)C(O)C(C)C(C)C2O1 WIGCFUFOHFEKBI-UHFFFAOYSA-N 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 125000002791 glucosyl group Chemical group C1([C@H](O)[C@@H](O)[C@H](O)[C@H](O1)CO)* 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 230000002607 hemopoietic effect Effects 0.000 description 1
- 201000001059 hepatic coma Diseases 0.000 description 1
- 231100000234 hepatic damage Toxicity 0.000 description 1
- 230000002440 hepatic effect Effects 0.000 description 1
- 208000007386 hepatic encephalopathy Diseases 0.000 description 1
- 230000004727 humoral immunity Effects 0.000 description 1
- 210000000987 immune system Anatomy 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 206010022437 insomnia Diseases 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 210000000629 knee joint Anatomy 0.000 description 1
- 229930013686 lignan Natural products 0.000 description 1
- 235000009408 lignans Nutrition 0.000 description 1
- 150000005692 lignans Chemical class 0.000 description 1
- 230000008818 liver damage Effects 0.000 description 1
- 230000033001 locomotion Effects 0.000 description 1
- WPBNNNQJVZRUHP-UHFFFAOYSA-L manganese(2+);methyl n-[[2-(methoxycarbonylcarbamothioylamino)phenyl]carbamothioyl]carbamate;n-[2-(sulfidocarbothioylamino)ethyl]carbamodithioate Chemical compound [Mn+2].[S-]C(=S)NCCNC([S-])=S.COC(=O)NC(=S)NC1=CC=CC=C1NC(=S)NC(=O)OC WPBNNNQJVZRUHP-UHFFFAOYSA-L 0.000 description 1
- 230000003340 mental effect Effects 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 239000013642 negative control Substances 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- 230000036407 pain Effects 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- SHUZOJHMOBOZST-UHFFFAOYSA-N phylloquinone Natural products CC(C)CCCCC(C)CCC(C)CCCC(=CCC1=C(C)C(=O)c2ccccc2C1=O)C SHUZOJHMOBOZST-UHFFFAOYSA-N 0.000 description 1
- 230000003389 potentiating effect Effects 0.000 description 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 235000018102 proteins Nutrition 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 239000002516 radical scavenger Substances 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000007420 reactivation Effects 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 230000003014 reinforcing effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000001624 sedative effect Effects 0.000 description 1
- 210000002460 smooth muscle Anatomy 0.000 description 1
- 150000003432 sterols Chemical class 0.000 description 1
- 235000003702 sterols Nutrition 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 229960003080 taurine Drugs 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 230000035922 thirst Effects 0.000 description 1
- 239000003053 toxin Substances 0.000 description 1
- 231100000765 toxin Toxicity 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 238000002604 ultrasonography Methods 0.000 description 1
- 210000001835 viscera Anatomy 0.000 description 1
- 235000019154 vitamin C Nutrition 0.000 description 1
- 239000011718 vitamin C Substances 0.000 description 1
- 235000019165 vitamin E Nutrition 0.000 description 1
- 229940046009 vitamin E Drugs 0.000 description 1
- 239000011709 vitamin E Substances 0.000 description 1
- 235000019168 vitamin K Nutrition 0.000 description 1
- 239000011712 vitamin K Substances 0.000 description 1
- 150000003721 vitamin K derivatives Chemical class 0.000 description 1
- 229940046010 vitamin k Drugs 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- 230000002087 whitening effect Effects 0.000 description 1
- 230000037303 wrinkles Effects 0.000 description 1
- 239000011701 zinc Substances 0.000 description 1
- 229910052725 zinc Inorganic materials 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L17/00—Food-from-the-sea products; Fish products; Fish meal; Fish-egg substitutes; Preparation or treatment thereof
- A23L17/50—Molluscs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/56—Materials from animals other than mammals
- A61K35/618—Molluscs, e.g. fresh-water molluscs, oysters, clams, squids, octopus, cuttlefish, snails or slugs
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/23—Apiaceae or Umbelliferae (Carrot family), e.g. dill, chervil, coriander or cumin
- A61K36/236—Ligusticum (licorice-root)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/25—Araliaceae (Ginseng family), e.g. ivy, aralia, schefflera or tetrapanax
- A61K36/254—Acanthopanax or Eleutherococcus
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/31—Brassicaceae or Cruciferae (Mustard family), e.g. broccoli, cabbage or kohlrabi
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/57—Magnoliaceae (Magnolia family)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/70—Polygonaceae (Buckwheat family), e.g. spineflower or dock
- A61K36/704—Polygonum, e.g. knotweed
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/79—Schisandraceae (Schisandra family)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/81—Solanaceae (Potato family), e.g. tobacco, nightshade, tomato, belladonna, capsicum or jimsonweed
- A61K36/815—Lycium (desert-thorn)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/894—Dioscoreaceae (Yam family)
- A61K36/8945—Dioscorea, e.g. yam, Chinese yam or water yam
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/906—Zingiberaceae (Ginger family)
- A61K36/9066—Curcuma, e.g. common turmeric, East Indian arrowroot or mango ginger
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/53—Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization
Landscapes
- Health & Medical Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Veterinary Medicine (AREA)
- Animal Behavior & Ethology (AREA)
- Botany (AREA)
- Public Health (AREA)
- Medicinal Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Mycology (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Medical Informatics (AREA)
- Alternative & Traditional Medicine (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Nutrition Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
本发明提供一种牡蛎保健食品及其制备方法，所述牡蛎保健食品包括以下重量份的组分：牡蛎30～50份、芜菁20～30份、刺五加10～15份、枸杞子20～25份、白豆10～20份、何首乌10～20份、川穹15～20份、郁金5～10份、五味子10～15份、山药5～15份。所述制备方法将各个组分的有效成分充分提取出来，提供抗疲劳、增强免疫力和养颜的作用。
Description
技术领域
本发明涉及保健食品技术领域，特别涉及一种牡蛎保健食品及其制备方法。
背景技术
牡蛎属软体动物门，含有丰富的糖原、蛋白质、氨基酸、脂肪、微量元素和维生素等，具有很高的使用和药用价值，在我国既是药品又可作为食品的保健疗效品，牡蛎是种营养非常丰富的食物，将其加工成保健食品，将营养成分提取制备出来，牡蛎具有降血脂，促进新陈代谢，提高免疫力等功效，满足人们的需求。牡蛎多糖具有增强机体细胞免疫和体液免疫功能，抗白细胞下降，抗肿瘤，抗血栓，降血脂等药用价值。糖原是一种仅有葡萄糖组成的特殊多糖，是动物体内能源物质的贮存形式，由于其独特的分子结构，使其在生理活性上不同于一般多糖，牡蛎糖原具有较好的抗疲劳活性，因此对于牡蛎糖原及其活性的研究具有重要的意义。通过研究与中药材的特殊搭配，使得与牡蛎结合发挥出最佳疗效，具有很好的保健效果。
发明内容
鉴以此，本发明提出一种牡蛎保健食品，提供抗疲劳、增强免疫力的作用。
本发明的技术方案是这样实现的：一种牡蛎保健食品，包括以下重量份的组分：牡蛎30～50份、芜菁20～30份、刺五加10～15份、枸杞子20～25份、白豆10～20份、何首乌10～20份、川穹15～20份、郁金5～10份、五味子10～15 份、山药5～15份。
优选地，包括以下重量份的组分：牡蛎40份、芜菁25份、刺五加12份、枸杞子23份、白豆16份、何首乌15份、川穹18份、郁金8份、五味子13份、山药11份。
本发明还包括一种牡蛎保健食品的制备方法，包括以下步骤：
(1)清洗：将牡蛎清洗干净，破碎进行干蒸备用；
(2)提取：牡蛎按料液比为1～3：15～20与水混合，超声波辅助水提取，提取温度为60～75℃，提取时间为2～3h，离心分离，取上清液；
(3)药材提取液的制备：称取芜菁、刺五加、枸杞子、白豆、何首乌、川穹、郁金、五味子和山药，粉碎，用乙醇回流提取，药材与乙醇用量的重量比为1～2：5～8，取提取液减压蒸馏，得药材提取液；
(4)调配：将上清液和药材提取液按重量比5～10：3～6进行混合均匀，得混合液；
(5)沉淀分离：将混合液浓缩加入乙醇，搅拌1～2h后，离心分离，取沉淀，加入有机溶剂20～30min后进行离心分离，取下层沉淀；
(6)纯化：将下层沉淀先用丙酮洗涤2～3次，再用乙醇洗涤3～5次；
(7)冷冻干燥：下层沉淀进行冷冻干燥，磨粉，制备成成品。
优选地，所述步骤(1)中，干蒸温度为90～110℃，干蒸时间为10～15min。
优选地，所述步骤(2)中，所述超声波工作功率为180～200W，工作每 15～20min后，暂停8～10min。
优选地，所述步骤(3)中，用85～90％乙醇回流提取2～3次，每次2～3h。
优选地，所述步骤(3)中，减压蒸馏中压力为80～100Pa，温度为30～35℃。
优选地，所述步骤(5)中，所述有机溶剂为氯仿、正丁醇和乙醇中的一种或两种。
优选地，所述步骤(6)中，将下层沉淀先用丙酮洗涤2次，再分别用80％、 85％、90％、95％的乙醇洗涤4次。
与现有技术相比，本发明的有益效果是：
牡蛎具有丰富的营养物质，含有丰富的糖原，其糖原对治疗抗疲劳、增强免疫力和美容养颜等具有重要的作用，与其它营养成分搭配，制备出的保健食品疗效好。以牡蛎、芜菁、刺五加和枸杞子为主料，增加糖原的含量，补充各种营养物质，对气血具有双向调节作用，能抵抗疲劳、增加免疫力和养颜的作用；何首乌、川穹为臣药，补气益血，健胃生精的作用，而郁金、五味子和山药为佐药，平补气阴，补五脏，安神等效果，白豆调和诸药，促进人体新陈代谢，健脾和胃，补气养血，达到抗疲劳、增强免疫力和养颜的作用。
牡蛎：味甘、咸，性平，能滋阴益血，养心安神，含糖元、牛磺酸、多种人本必须氨基酸，维生素A、B1、B2、D、E，铜、锌、锰、钡、磷、钙等成分，用于虚损劳疾，阴虚血亏，失眠心悸。
芜菁：芜菁富含维生素A，叶酸，维生素C，维生素K和钙。开胃下气，利湿解毒。治食积不化，黄疸，消渴，热毒风肿。
刺五加：益气健脾，补肾安神。用于脾肾阳虚、体虚乏力、食欲不振、腰膝酸痛、失眠多梦。补气：可提高人体的氧气吸收量。生血：可改善大脑血量，对血压具有双向调节作用。同时还可以抗疲劳、抗辐射、补虚弱、增强骨髓造血功能，并具有活血作用。安神：人体在氧气吸收不足时，容易昏沉、打哈欠、产生卷怠感，刺五加为有氧物质，可以调节如中枢神经系统的兴奋和抑制过程，使二者趋于平衡，有益智和安神作用。强身：可以提升最大耗氧量，增强运动的持续时间。
枸杞子：可以增加肌糖原和肝糖原的含量，提高活力，抗疲劳，能够增强机体免疫力和对各种有害刺激的适应力，抵御病症，固本培元。枸杞还可以有效增强骨髓的造血功能，提高机体血液和肝的超氧化歧化酶活性含量。有助于清除活性氧，有效抵抗衰老和疲劳。
白豆：甘咸，平，调中益气，健脾益肾。补五脏，调中，助十二经脉。
何首乌：苦、甘、涩，温。归肝、心、肾经。促进造血功能，增强免疫功能，降血脂与抗动脉粥样硬化，保肝，延缓衰老，现代调查有维生素E制止皱纹再生，对内分泌的影响，润肠通便。
川穹：活血行气、祛风止痛，镇静，对心血管、平滑肌、抗菌具有良好疗效。
郁金：辛、苦，寒。归肝、心、肺经，凉心热，散肝郁，行气解郁，凉血破瘀，治胸腹胁肋诸痛，失心癫狂，热病神昏。
五味子：温；酸、甘；归肺、心、肾经，五味子含有丰富的有机酸、维生素、类黄酮、植物固醇及有强效复原作用的木酚素，保肝及再生肝脏组织，具有消炎作用，以制止肝脏损伤，能增强耐力与力气并加速体力复原。
山药：健脾益胃、助消化，降低血糖，抗肝昏迷，助五脏，强筋骨，长志安神。
具体实施方式
为了更好理解本发明技术内容，下面提供具体实施例，并对本发明做进一 步的说明。
实施例1
一种牡蛎保健食品，包括以下重量的组分：牡蛎30g、芜菁20g、刺五加10g、枸杞子20g、白豆10g、何首乌10g、川穹15g、郁金5g、五味子10g、山药5g。
实施例2
一种牡蛎保健食品，包括以下重量的组分：牡蛎50g、芜菁30g、刺五加15g、枸杞子25g、白豆20g、何首乌20g、川穹20g、郁金10g、五味子15g、山药15g。
实施例3
一种牡蛎保健食品，包括以下重量的组分：牡蛎40g、芜菁25g、刺五加12g、枸杞子23g、白豆16g、何首乌15g、川穹18g、郁金8g、五味子13g、山药11g。
上述实施例1～3所述牡蛎保健食品的制备方法，包括以下步骤：
(1)清洗：将牡蛎清洗干净，破碎进行干蒸备用，干蒸温度为100℃，干蒸时间为13min。
(2)提取：牡蛎按料液比为2：18与水混合，超声波辅助水提取，所述超声波工作功率为190W，工作每18min后，暂停9min。提取温度为70℃，提取时间为2.5h，离心分离，取上清液。
(3)药材提取液的制备：称取芜菁、刺五加、枸杞子、白豆、何首乌、川穹、郁金、五味子和山药，粉碎，用85％乙醇回流提取3次，每次2h，药材与乙醇用量的重量比为2：7，取提取液减压蒸馏，压力为90Pa，温度为32℃，得药材提取液。
(4)调配：将上清液和药材提取液按重量比8：5进行混合均匀，得混合液。
(5)沉淀分离：将混合液浓缩加入乙醇，搅拌1.5h后，离心分离，取沉淀，加入氯仿和正丁醇有机溶剂25min后进行离心分离，取下层沉淀。
(6)纯化：将下层沉淀先用丙酮洗涤2次，再分别用80％、85％、90％、 95％的乙醇洗涤4次。
(7)冷冻干燥：下层沉淀进行冷冻干燥，磨粉，制备成成品。
实施例4
该实施例与实施例3的区别在于：所述牡蛎保健食品的制备方法，包括以下步骤：
(1)清洗：将牡蛎清洗干净，破碎进行干蒸备用，干蒸温度为90℃，干蒸时间为10min。
(2)提取：牡蛎按料液比为1：15与水混合，超声波辅助水提取，所述超声波工作功率为180W，工作每15min后，暂停8min。提取温度为60℃，提取时间为2h，离心分离，取上清液。
(3)药材提取液的制备：称取芜菁、刺五加、枸杞子、白豆、何首乌、川穹、郁金、五味子和山药，粉碎，用85％乙醇回流提取2次，每次2h，药材与乙醇用量的重量比为1：5，取提取液减压蒸馏，压力为80Pa，温度为30℃，得药材提取液。
(4)调配：将上清液和药材提取液按重量比5：3进行混合均匀，得混合液。
(5)沉淀分离：将混合液浓缩加入乙醇，搅拌1h后，离心分离，取沉淀，加入氯仿有机溶剂20min后进行离心分离，取下层沉淀。
(6)纯化：将下层沉淀先用丙酮洗涤2次，再分别用80％、85％、95％的乙醇洗涤3次。
(7)冷冻干燥：下层沉淀进行冷冻干燥，磨粉，制备成成品。
实施例5
该实施例与实施例3的区别在于：所述牡蛎保健食品的制备方法，包括以下步骤：
(1)清洗：将牡蛎清洗干净，破碎进行干蒸备用，干蒸温度为110℃，干蒸时间为15min。
(2)提取：牡蛎按料液比为3：20与水混合，超声波辅助水提取，所述超声波工作功率为200W，工作每20min后，暂停10min。提取温度为75℃，提取时间为3h，离心分离，取上清液。
(3)药材提取液的制备：称取芜菁、刺五加、枸杞子、白豆、何首乌、川穹、郁金、五味子和山药，粉碎，用90％乙醇回流提取3次，每次3h，药材与乙醇用量的重量比为2：8，取提取液减压蒸馏，压力为100Pa，温度为35℃，得药材提取液。
(4)调配：将上清液和药材提取液按重量比10：6进行混合均匀，得混合液。
(5)沉淀分离：将混合液浓缩加入乙醇，搅拌2h后，离心分离，取沉淀，加入乙醇有机溶剂30min后进行离心分离，取下层沉淀。
(6)纯化：将下层沉淀先用丙酮洗涤3次，再分别用80％、85％、85％、 90％、95％乙醇洗涤5次。
(7)冷冻干燥：下层沉淀进行冷冻干燥，磨粉，制备成成品。
其中，干蒸为的是将牡蛎进行去腥、使牡蛎变软，从而加速挥发出有害物质。超声波辅助水提取，使得原料易破碎，从而有助于多糖的溶出，提高多糖的得率，先工作后暂停是为了减少提取时间，维持提取温度的作用。减压蒸馏中避免加热引起生物活性破坏。将上清液与药材提取液按照一定比例调配，使得有效成分含量高。
本发明还设置以下对比例实验：
对比例1
该对比例与实施例3的区别在于：所述牡蛎保健食品的制备方法，包括以下步骤：
(1)清洗：将牡蛎清洗干净，进行破碎备用。
(2)提取：牡蛎按料液比为2：18与水混合，进行水提取，所述提取温度为70℃，提取时间为2.5h，离心分离，取上清液。
对比例2
该对比例与实施例3的区别在于：所述牡蛎保健食品的制备方法，包括以下步骤：
(3)药材提取液的制备：称取白豆、何首乌、川穹、郁金、五味子和山药，粉碎，用85％乙醇回流提取3次，每次2h，药材与乙醇用量的重量比为2：7，取提取液减压蒸馏，压力为90Pa，温度为32℃，得药材提取液。
(4)调配：将上清液和药材提取液按重量比12：8进行混合均匀，得混合液。
对比例3
该对比例与实施例3的区别在于：所述牡蛎保健食品的制备方法，包括以下步骤：
(5)沉淀分离：将混合液浓缩加入乙醇，搅拌1.5h后，离心分离，取下层沉淀。
(6)纯化：将下层沉淀用85％的乙醇洗涤1次。
一、抗疲劳效果实验：
1、受试动物：健康小鼠，体重20～25g，雌雄各半。
2、给药方式：
对照组：蒸馏水1.5mL/10g灌胃给药，一天两次，连续30天；
阳性组：市售牡蛎保健食品；
实验组：实施例1～5制备的成品配置成溶液1.5mL/10g灌胃给药，一天两次，连续30天。
对比组：对比例1～3制备的成品配置成溶液1.5mL/10g灌胃给药，一天两次，连续30天。
3、实验方法及结果分析
3.1抗疲劳实验-游泳法
末次灌胃后30min，负重投入水中，观察记录小鼠游泳力竭时间如下表1 所示：
| 组别 | 动物数 | 游泳时间(min) |
| 对照组 | 10 | 5.8 |
| 阳性组 | 10 | 7.1 |
| 实验组1 | 10 | 7.5 |
| 实验组2 | 10 | 7.8 |
| 实验组3 | 10 | 8.2 |
| 实验组4 | 10 | 7.4 |
| 实验组5 | 10 | 8.0 |
| 对比组1 | 10 | 6.0 |
| 对比组2 | 10 | 6.2 |
| 对比组3 | 10 | 6.4 |
由上表可知，与空白对照组比较，实验组明显提高游泳时间，而对比组相对于实验组其游泳时间较短，实验组的效果最优。
3.2抗缺氧实验
末次灌胃后30min，将小鼠投入密闭容器中密闭，记录小鼠存活时间如下表 2所示：
由表1和表2可知，本发明制备的牡蛎保健食品具有良好的抗疲劳效果。
二、祛斑美白实验
1、随机选取240名22～40岁长有黄褐斑、皮肤黄的女性志愿者，每组30 名，受试者身体状况良好，在服药期间没有不良嗜好。
2、祛斑效果判断
(1)痊愈：黄褐斑颜色明显变浅，甚至消失，且不产生新的黄褐斑，皮肤具有光泽红润；
(2)显效：黄褐斑颜色变浅，面积减小了一半，不产生新的黄褐斑，皮肤有光泽；
(3)有效：黄褐斑颜色变浅，面积开始减小，不产生新的黄褐斑，皮肤有光泽红润；
(4)无效：黄褐斑没有消失，皮肤暗黄。
3、将实施例1～5和对比例1～3制备的成品，验证无毒性后，早晚一次，连续口服5个月后，记录实验结果如下表3所示：
相对于对比组，实验组祛斑养颜的治愈率和有效率均比对比组的高，本发明制备的成品具有很好的祛色斑及面色红润有光泽的效果。
三、免疫力功能实验
1、将实施例1～5和对比例1～3制备的成品配置成溶液进行灌胃，阴性对照组给予等体积水，实验每组20只小鼠，每天灌胃1次，连续60d，实验终末，处死小鼠，对小鼠细胞免疫、体液免疫和NK细胞活性进行分析检测。
2、检测实验结果如下表4所示：
| 组别 | 淋巴细胞增殖能力(OD值) | 抗体积数 | NK细胞活性(％) |
| 对照组1 | 0.33 | 192.0 | 35.43 |
| 实验组1 | 0.63 | 235.4 | 49.43 |
| 实验组2 | 0.61 | 231.9 | 50.28 |
| 实验组3 | 0.68 | 254.3 | 52.36 |
| 实验组4 | 0.58 | 246.5 | 50.28 |
| 实验组5 | 0.62 | 238.4 | 51.67 |
| 对比组1 | 0.42 | 200.3 | 38.2 |
| 对比组2 | 0.38 | 198.4 | 38.1 |
| 对比组3 | 0.43 | 201.6 | 40.2 |
由上表可知，实验组细胞增殖能力、抗体积数和NK细胞活性均高于对照组，表明了本发明具有全面提高疗效的作用，牡蛎等结合其它组分的有效成分起到调节内分泌，营养机体，促进机体新陈代谢，促进细胞生长发育等功能，且增强整个免疫系统的功能。具有抗疲劳、美容养颜和增强免疫力的作用。
牡蛎具有丰富的营养物质，含有丰富的糖原，其糖原对治疗抗疲劳、增强免疫力和美容养颜等具有重要的作用，与其它营养成分搭配，制备出的保健食品疗效好。以牡蛎、芜菁、刺五加和枸杞子为主料，增加糖原的含量，补充各种营养物质，对气血具有双向调节作用，能抵抗疲劳、增加免疫力和养颜的作用；何首乌、川穹为臣药，补气益血，健胃生精的作用，而郁金、五味子和山药为佐药，平补气阴，补五脏，安神等效果，白豆调和诸药，促进人体新陈代谢，健脾和胃，补气养血，达到抗疲劳、增强免疫力和养颜的作用。
以上所述仅为本发明的较佳实施例而已，并不用以限制本发明，凡在本发明的精神和原则之内，所作的任何修改、等同替换、改进等，均应包含在本发明的保护范围之内。
Claims (9)
1.一种牡蛎保健食品，其特征在于，包括以下重量份的组分：牡蛎30～50份、芜菁20～30份、刺五加10～15份、枸杞子20～25份、白豆10～20份、何首乌10～20份、川穹15～20份、郁金5～10份、五味子10～15份、山药5～15份。
2.根据权利要求1所述的一种牡蛎保健食品，其特征在于，所述牡蛎保健食品包括以下重量份的组分：牡蛎40份、芜菁25份、刺五加12份、枸杞子23份、白豆16份、何首乌15份、川穹18份、郁金8份、五味子13份、山药11份。
3.根据权利要求1或2所述的一种牡蛎保健食品的制备方法，其特征在于，包括以下步骤：
(1)清洗：将牡蛎清洗干净，破碎进行干蒸备用；
(2)提取：牡蛎按料液比为1～3：15～20与水混合，超声波辅助水提取，提取温度为60～75℃，提取时间为2～3h，离心分离，取上清液；
(3)药材提取液的制备：称取芜菁、刺五加、枸杞子、白豆、何首乌、川穹、郁金、五味子和山药，粉碎，用乙醇回流提取，药材与乙醇用量的重量比为1～2：5～8，取提取液减压蒸馏，得药材提取液；
(4)调配：将上清液和药材提取液按重量比5～10：3～6进行混合均匀，得混合液；
(5)沉淀分离：将混合液浓缩加入乙醇，搅拌1～2h后，离心分离，取沉淀，加入有机溶剂20～30min后进行离心分离，取下层沉淀；
(6)纯化：将下层沉淀先用丙酮洗涤2～3次，再用乙醇洗涤3～5次；
(7)冷冻干燥：下层沉淀进行冷冻干燥，磨粉，制备成成品。
4.根据权利要求3所述的一种牡蛎保健食品的制备方法，其特征在于，所述步骤(1)中，干蒸温度为90～110℃，干蒸时间为10～15min。
5.根据权利要求3所述的一种牡蛎保健食品的制备方法，其特征在于，所述步骤(2)中，所述超声波工作功率为180～200W，工作每15～20min后，暂停8～10min。
6.根据权利要求3所述的一种牡蛎保健食品的制备方法，其特征在于，所述步骤(3)中，用85～90％乙醇回流提取2～3次，每次2～3h。
7.根据权利要求3所述的一种牡蛎保健食品的制备方法，其特征在于，所述步骤(3)中，减压蒸馏中压力为80～100Pa，温度为30～35℃。
8.根据权利要求3所述的一种牡蛎保健食品的制备方法，其特征在于，所述步骤(5)中，所述有机溶剂为氯仿、正丁醇和乙醇中的一种或两种。
9.根据权利要求3所述的一种牡蛎保健食品的制备方法，其特征在于，所述步骤(6)中，将下层沉淀先用丙酮洗涤2次，再分别用80％、85％、90％、95％的乙醇洗涤4次。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710960244.8A CN107751856A (zh) | 2017-10-16 | 2017-10-16 | 一种牡蛎保健食品及其制备方法 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710960244.8A CN107751856A (zh) | 2017-10-16 | 2017-10-16 | 一种牡蛎保健食品及其制备方法 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107751856A true CN107751856A (zh) | 2018-03-06 |
Family
ID=61268765
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710960244.8A Pending CN107751856A (zh) | 2017-10-16 | 2017-10-16 | 一种牡蛎保健食品及其制备方法 |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107751856A (zh) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109984317A (zh) * | 2019-04-25 | 2019-07-09 | 温州科技职业学院 | 一种芜菁保健食品及其制备方法 |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1745790A (zh) * | 2005-09-01 | 2006-03-15 | 赵东科 | 一种治疗虚劳的中药制剂及制备方法 |
| KR100863490B1 (ko) * | 2007-03-30 | 2008-10-15 | 한국식품연구원 | 레토르트 매생이탕의 제조방법 |
| CN102048902A (zh) * | 2010-12-22 | 2011-05-11 | 中国人民解放军第三○二医院 | 一种中药组合物、提取物及制备方法、应用和剂型 |
| CN102973685A (zh) * | 2012-12-25 | 2013-03-20 | 刘国林 | 一种具有抗疲劳的中药制剂及其制备方法 |
| CN105327238A (zh) * | 2014-07-04 | 2016-02-17 | 浙江爱生药业有限公司 | 一种健脾养胃的中药组合物及其制备方法和用途 |
| CN106668562A (zh) * | 2015-11-10 | 2017-05-17 | 重庆都好生物科技有限公司 | 一种养肝护肝冲剂 |
-
2017
- 2017-10-16 CN CN201710960244.8A patent/CN107751856A/zh active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1745790A (zh) * | 2005-09-01 | 2006-03-15 | 赵东科 | 一种治疗虚劳的中药制剂及制备方法 |
| KR100863490B1 (ko) * | 2007-03-30 | 2008-10-15 | 한국식품연구원 | 레토르트 매생이탕의 제조방법 |
| CN102048902A (zh) * | 2010-12-22 | 2011-05-11 | 中国人民解放军第三○二医院 | 一种中药组合物、提取物及制备方法、应用和剂型 |
| CN102973685A (zh) * | 2012-12-25 | 2013-03-20 | 刘国林 | 一种具有抗疲劳的中药制剂及其制备方法 |
| CN105327238A (zh) * | 2014-07-04 | 2016-02-17 | 浙江爱生药业有限公司 | 一种健脾养胃的中药组合物及其制备方法和用途 |
| CN106668562A (zh) * | 2015-11-10 | 2017-05-17 | 重庆都好生物科技有限公司 | 一种养肝护肝冲剂 |
Non-Patent Citations (2)
| Title |
|---|
| 李志: "牡蛎多糖的分离纯化及生物学活性研究", 《中国优秀硕士学位论文全文数据库·医药卫生科技辑》 * |
| 高溥超: "《肝炎康复食谱》", 31 March 2000, 合肥：安徽人民出版社 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109984317A (zh) * | 2019-04-25 | 2019-07-09 | 温州科技职业学院 | 一种芜菁保健食品及其制备方法 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN107296949A (zh) | 药食同源复合制剂及其制备方法和应用 | |
| CN101904503A (zh) | 五行营养餐及其制备工艺 | |
| CN103783382A (zh) | 一种防脱发的保健粥及其制备方法 | |
| CN103859375A (zh) | 一种有助于增强免疫力的养生保健食品配方及其制备方法 | |
| CN106072527A (zh) | 一种排毒养颜的纳豆组方及其制备方法 | |
| CN106360286A (zh) | 一种营养组合物及其制备方法和应用 | |
| CN108991078A (zh) | 一种防控富贵病的桑叶食品及其制备方法 | |
| CN105994748A (zh) | 老青海青稞酥油茶 | |
| CN101113401A (zh) | 一种黑豆养生醋 | |
| CN104109609A (zh) | 糯米酒荞麦果汁饮料及其制备方法 | |
| CN103960426B (zh) | 一种中药减肥茶 | |
| CN102813206A (zh) | 一种具有补脑益智功效的食品组合物及其制备方法 | |
| CN109275856A (zh) | 一种灵芝食品 | |
| CN104522572A (zh) | 一种补气益脾的葛根粉 | |
| CN107751856A (zh) | 一种牡蛎保健食品及其制备方法 | |
| CN105559078A (zh) | 一种具有补血功效的组合物及其制备方法 | |
| CN109527577A (zh) | 一种具有解酒护肝功能的组合物 | |
| CN107319534A (zh) | 一种抗疲劳和养颜的保健食品及其制备方法 | |
| CN106135465A (zh) | 一种螺旋藻滋补固本营养油及其制备方法 | |
| CN104207121B (zh) | 一种提高免疫力降低三高的食疗保健品 | |
| CN105876257A (zh) | 一种含有葛的鱼饲料添加剂及其鱼饲料以及使用方法 | |
| CN109363028A (zh) | 一种缓解体力疲劳的西洋参饮品制备方法 | |
| CN105533522A (zh) | 一种增强免疫力祛除口臭和狐臭的食品的制作方法 | |
| CN105214068B (zh) | 一种治疗小儿多发性抽动症的营养组合物及其制备方法 | |
| KR20210084942A (ko) | 체질 맞춤형의 학습 능력 증진용 건강 기능성 식품 조성물 제조방법 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20180306 |
|
| RJ01 | Rejection of invention patent application after publication |