CN107750170B - 转分化的方法和其使用方法 - Google Patents

转分化的方法和其使用方法 Download PDF

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Publication number
CN107750170B
CN107750170B CN201580077116.8A CN201580077116A CN107750170B CN 107750170 B CN107750170 B CN 107750170B CN 201580077116 A CN201580077116 A CN 201580077116A CN 107750170 B CN107750170 B CN 107750170B
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cells
cell
expression
transdifferentiation
insulin
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CN107750170A (zh
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S·费贝尔
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Tel HaShomer Medical Research Infrastructure and Services Ltd
Orgenesis Ltd
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Tel HaShomer Medical Research Infrastructure and Services Ltd
Orgenesis Ltd
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CN201580077116.8A 2014-12-30 2015-12-30 转分化的方法和其使用方法 Expired - Fee Related CN107750170B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201462098050P 2014-12-30 2014-12-30
US62/098,050 2014-12-30
PCT/IL2015/051267 WO2016108237A1 (en) 2014-12-30 2015-12-30 Methods of transdifferentiation and methods of use thereof

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CN107750170A CN107750170A (zh) 2018-03-02
CN107750170B true CN107750170B (zh) 2021-10-08

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US (3) US10179151B2 (https=)
EP (2) EP3240576B1 (https=)
JP (1) JP6817943B2 (https=)
KR (1) KR102487883B1 (https=)
CN (1) CN107750170B (https=)
AU (1) AU2015373129B2 (https=)
BR (1) BR112017014280A2 (https=)
CA (1) CA2972569A1 (https=)
CO (1) CO2017006937A2 (https=)
EA (1) EA035360B1 (https=)
IL (1) IL253234B (https=)
MA (1) MA41296A (https=)
MX (1) MX378321B (https=)
SG (1) SG11201705344VA (https=)
WO (1) WO2016108237A1 (https=)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP6650393B2 (ja) 2013-06-13 2020-02-19 オージェネシス リミテッド 細胞の集団、分化転換の方法及びその使用方法
SG11201602232UA (en) 2013-09-24 2016-04-28 Giner Inc System for gas treatment of a cell implant
MA41296A (fr) 2014-12-30 2017-11-07 Orgenesis Ltd Procédés de transdifférenciation et procédés d'utilisation de ceux-ci
JP6987080B2 (ja) 2016-05-05 2021-12-22 サウスウェスト リサーチ インスティテュート 細胞増殖のための三次元バイオリアクター及び関連用途
AU2017361260B2 (en) 2016-11-15 2023-06-08 Giner, Inc. Percutaneous gas diffusion device suitable for use with a subcutaneous implant
CN110831656A (zh) 2017-05-04 2020-02-21 吉纳生命科学公司 稳健的可植入气体递送装置和方法、系统以及包括该装置的装置
EP3635106A4 (en) * 2017-05-08 2021-01-06 Orgenesis Ltd. POPULATIONS OF TRANSDIFFERENTIATED CELLS AND THEIR METHODS OF USE
KR20200018506A (ko) * 2017-05-29 2020-02-19 오르제네시스 엘티디. 세포 대체 요법을 제공하기 위한 조성물 및 방법
JP2020537522A (ja) 2017-10-13 2020-12-24 イーエムベーアー−インスティテュート フュール モレクラレ バイオテクノロジー ゲゼルシャフト ミット ベシュレンクテル ハフツング 体細胞の増強された再プログラミング
WO2019171377A1 (en) * 2018-03-06 2019-09-12 Orgenesis Inc Three dimensional clusters of transdifferentiated cells, compositions and methods thereof
US11149244B2 (en) 2018-04-04 2021-10-19 Southwest Research Institute Three-dimensional bioreactor for T-cell activation and expansion for immunotherapy
JP7376576B2 (ja) 2018-09-24 2023-11-08 サウスウェスト リサーチ インスティテュート 三次元バイオリアクター
EP3816276A1 (en) * 2019-10-31 2021-05-05 Unicyte Islet AG Viable pancreatic islet-like cell structures and a method of preparing thereof
AU2022377625B2 (en) 2021-10-28 2025-01-02 Southwest Research Institute Devices for cell separation

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008013737A2 (en) * 2006-07-19 2008-01-31 University Of Florida Research Foundation, Inc. Compositions for reprogramming a cell and uses therefor
WO2010022395A2 (en) * 2008-08-22 2010-02-25 President And Fellows Of Harvard College Methods of reprogramming cells
WO2014207578A3 (en) * 2013-06-13 2015-09-11 Orgenesis Ltd. Cell populations, methods of transdifferention and methods of use thereof

Family Cites Families (68)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4522811A (en) 1982-07-08 1985-06-11 Syntex (U.S.A.) Inc. Serial injection of muramyldipeptides and liposomes enhances the anti-infective activity of muramyldipeptides
US4980286A (en) 1985-07-05 1990-12-25 Whitehead Institute For Biomedical Research In vivo introduction and expression of foreign genetic material in epithelial cells
US4737323A (en) 1986-02-13 1988-04-12 Liposome Technology, Inc. Liposome extrusion method
DE122007000007I2 (de) 1986-04-09 2010-12-30 Genzyme Corp Genetisch transformierte Tiere, die ein gewünschtes Protein in Milch absondern
US4837028A (en) 1986-12-24 1989-06-06 Liposome Technology, Inc. Liposomes with enhanced circulation time
US4873316A (en) 1987-06-23 1989-10-10 Biogen, Inc. Isolation of exogenous recombinant proteins from the milk of transgenic mammals
US4992417A (en) 1987-07-17 1991-02-12 Mount Sinai School Of Medicine Superactive human insulin analogues
US5082670A (en) 1988-12-15 1992-01-21 The Regents Of The University Of California Method of grafting genetically modified cells to treat defects, disease or damage or the central nervous system
US5703055A (en) 1989-03-21 1997-12-30 Wisconsin Alumni Research Foundation Generation of antibodies through lipid mediated DNA delivery
US5328470A (en) 1989-03-31 1994-07-12 The Regents Of The University Of Michigan Treatment of diseases by site-specific instillation of cells or site-specific transformation of cells and kits therefor
US5427940A (en) 1991-06-03 1995-06-27 Board Of Regents, The University Of Texas System Engineered cells producing insulin in response to glucose
ES2157225T3 (es) 1992-10-09 2001-08-16 Advanced Tissue Sciences Inc Celulas hepaticas de reserva.
US5523226A (en) 1993-05-14 1996-06-04 Biotechnology Research And Development Corp. Transgenic swine compositions and methods
US5424286A (en) 1993-05-24 1995-06-13 Eng; John Exendin-3 and exendin-4 polypeptides, and pharmaceutical compositions comprising same
JP3802051B2 (ja) 1993-08-16 2006-07-26 リサーチ ディベロップメント ファンデーション 膵臓島細胞におけるインシュリン表現を刺激する新しいホメオボックス因子
US5858973A (en) 1994-02-23 1999-01-12 The General Hospital Corporation Transcription factor and uses therefor
IL114193A (en) 1994-06-20 2000-02-29 Teva Pharma Ophthalmic pharmaceutical compositions based on sodium alginate
US5849989A (en) 1994-10-07 1998-12-15 Ontogeny, Inc. Insulin promoter factor, and uses related thereto
JP2000501290A (ja) 1995-11-30 2000-02-08 リサーチ ディベロップメント ファンデーション ソマトスタチン及びインシュリン生成を刺激する化合物のスクリーニング・アッセイ
IL118376A0 (en) 1996-05-22 1996-09-12 Univ Ben Gurion Polysaccharide sponges for cell culture and transplantation
JP2000513227A (ja) 1996-06-24 2000-10-10 ビーボウアールエックス ファーマスーティカルズ,インコーポレイテッド インスリン分泌細胞の単離および増殖方法
EP0996736A1 (en) 1997-08-11 2000-05-03 Chiron Corporation Methods for genetically modifying t cells
CA2221195A1 (en) 1997-11-14 1999-05-14 Chantal E. Holy Biodegradable polymer matrix
US6143293A (en) 1998-03-26 2000-11-07 Carnegie Mellon Assembled scaffolds for three dimensional cell culturing and tissue generation
IL124957A0 (en) 1998-06-16 1999-01-26 Univ Ben Gurion Active ingredient delivery systems and devices based on porous matrices
US6242666B1 (en) 1998-12-16 2001-06-05 The Scripps Research Institute Animal model for identifying a common stem/progenitor to liver cells and pancreatic cells
US8778899B2 (en) 1999-06-01 2014-07-15 Sarah Ferber Methods of inducing regulated pancreatic hormone production in non-pancreatic islet tissues
US6774120B1 (en) 1999-06-01 2004-08-10 Sarah Ferber Methods of inducing regulated pancreatic hormone production in non-pancreatic islet tissues
US6716824B1 (en) 1999-10-22 2004-04-06 F. Charles Brunicardi Treatment of pancreatic adenocarcinoma by cytotoxic gene therapy
US6759039B2 (en) 2000-06-30 2004-07-06 Amcyte, Inc. Culturing pancreatic stem cells having a specified, intermediate stage of development
US7214371B1 (en) 2000-09-01 2007-05-08 Ben-Gurion University Of The Negev Research & Development Authority Tissue engineered biografts for repair of damaged myocardium
WO2003020894A2 (en) 2001-08-31 2003-03-13 Joslin Diabetes Center, Inc. Insulin related transcription factor and uses thereof
CA2463914A1 (en) 2001-10-18 2003-04-24 Ixion Biotechnology, Inc. Conversion of liver stem and progenitor cells to pancreatic functional cells
US7722894B2 (en) 2001-10-22 2010-05-25 Massachusetts Institute Of Technology Biodegradable polymer
IL162131A0 (en) 2001-12-07 2005-11-20 Geron Corp Islet cells from human embryonic stem cells
AU2003217658A1 (en) * 2002-02-22 2003-09-09 University Of Florida Cellular trans-differentiation
GB0206357D0 (en) 2002-03-18 2002-05-01 Univ Bath Cells
JP4136434B2 (ja) 2002-04-17 2008-08-20 進 清野 インスリン産生細胞の誘導
WO2003102171A1 (en) 2002-05-28 2003-12-11 Becton, Dickinson And Company Expansion and transdifferentiation of human acinar cells
KR101180896B1 (ko) 2003-05-05 2012-09-07 벤-구리온 유니버시티 오브 더 네게브 리서치 앤드 디벨럽먼트 어쏘러티 주사용 가교결합된 폴리머 제제 및 그의 용도
AU2004236573B2 (en) 2003-05-12 2009-10-22 Sarah Ferber Methods of inducing regulated pancreatic hormone production in non-pancreatic islet tissues
WO2005005607A2 (en) 2003-06-30 2005-01-20 Lifescan, Inc. Seeding pancreatic cells on porous matrices
CA2536067A1 (en) 2003-08-27 2005-03-10 Stemcells California, Inc. Enriched pancreatic stem cell and progenitor cell populations, and methods for identifying, isolating and enriching for these populations
CA2548464C (en) 2003-12-09 2013-02-12 Ben-Gurion University Of The Negev Research And Development Authority Pulse-medium perfusion bioreactor with improved mass transport for multiple 3-d cell constructs
EP1784481A1 (en) 2004-08-25 2007-05-16 Technion Research And Development Foundation, Ltd. Methods of generating embryoid bodies using three dimensional scaffolds
WO2006092799A1 (en) 2005-03-02 2006-09-08 Polyrizon Ltd. Method for removal of toxins from mucosal membranes
EP1856246B1 (en) 2005-03-07 2015-07-15 Technion Research & Development Foundation Limited Natural tissue-derived decellularized matrix and methods of generating and using same
US7517856B2 (en) 2005-10-11 2009-04-14 Ben-Gurion University Of The Negev Research And Development Authority Bioconjugates comprising sulfated polysaccharides and their uses
WO2008075339A2 (en) 2006-12-18 2008-06-26 Ben Gurion University Of The Negev Scaffolding for tissue regeneration or repair
WO2009007979A2 (en) 2007-07-11 2009-01-15 Technion Research & Development Foundation Ltd. Encapsulated mesenchymal stem cells and uses thereof
IL187707A0 (en) 2007-11-27 2008-11-03 Univ Ben Gurion Alginate scaffold in hepatectomy
WO2009126927A2 (en) * 2008-04-11 2009-10-15 Joslin Diabetes Center, Inc. Methods of generating insulin-producing cells
CN102858380B (zh) 2009-08-02 2016-03-23 学校法人东京女子医科大学 胰岛细胞片、制造方法及其利用方法
KR101874463B1 (ko) * 2009-10-31 2018-08-02 뉴 월드 레보러토리즈 인코포레이티드. 세포의 재프로그램화 방법 및 이의 용도
JP2011223993A (ja) 2010-03-30 2011-11-10 Kumamoto Univ 幹細胞の分化誘導方法
WO2011159726A2 (en) 2010-06-14 2011-12-22 The Scripps Research Institute Reprogramming of cells to a new fate
EP2741753A2 (en) 2011-08-09 2014-06-18 Yeda Research and Development Co. Ltd. Downregulation of mir-7 for promotion of beta cell differentiation and insulin production
EP2816964B1 (en) 2012-02-21 2023-12-27 Ben-Gurion University of The Negev Research and Development Authority Hydrogel system comprising spatially separated bioactive polypeptides
US9993497B2 (en) 2012-12-30 2018-06-12 Hadasit Medical Research Services And Development Ltd. Use of alginate compositions in preventing or reducing liver damage caused by a hepatotoxic agent
JP6490669B2 (ja) 2013-05-08 2019-03-27 インリージェン 単離腎細胞を含有するオルガノイド及びその使用
EP3107549B1 (en) 2014-02-20 2020-07-01 B.G. Negev Technologies and Applications Ltd. Anionic polyplexes for use in the delivery of nucleic acids
EP3149067B1 (en) 2014-05-30 2021-03-17 The Secant Group, LLC Water-mediated preparations of polymeric materials
EP3149026B1 (en) 2014-06-02 2025-03-05 B.G. Negev Technologies and Applications Ltd. Site-selective modification of polysaccharides and applications thereof
MA41296A (fr) 2014-12-30 2017-11-07 Orgenesis Ltd Procédés de transdifférenciation et procédés d'utilisation de ceux-ci
WO2017118979A1 (en) 2016-01-07 2017-07-13 B. G. Negev Technologies And Applications Ltd., At Ben-Gurion University Compositions and methods for generating immunotolerant responses
WO2017175229A1 (en) 2016-04-07 2017-10-12 B.G. Negev Technologies And Applications Ltd. Polysaccharide compositions and uses thereof
US20170290954A1 (en) 2016-04-07 2017-10-12 B. G. Negev Technologies And Applications Ltd., At Ben-Gurion University Compositions and methods for treating spinal cord injury
EP3635106A4 (en) 2017-05-08 2021-01-06 Orgenesis Ltd. POPULATIONS OF TRANSDIFFERENTIATED CELLS AND THEIR METHODS OF USE

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008013737A2 (en) * 2006-07-19 2008-01-31 University Of Florida Research Foundation, Inc. Compositions for reprogramming a cell and uses therefor
CN101553245A (zh) * 2006-07-19 2009-10-07 佛罗里达大学研究基金会有限公司 用于细胞重编程的组合物及其用途
WO2010022395A2 (en) * 2008-08-22 2010-02-25 President And Fellows Of Harvard College Methods of reprogramming cells
WO2014207578A3 (en) * 2013-06-13 2015-09-11 Orgenesis Ltd. Cell populations, methods of transdifferention and methods of use thereof

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
Human liver cells expressing albumin and mesenchymal characteristics give rise to insulin-producing cells;Meivar-Levy, Irit等;《Journal of transplantation》;20110824;全文 *
The role of the Wnt signaling pathway in incretin hormone production and function;Yu-ting A., Wilfred Ip, and Tianru Jin等;《Frontiers In physiology》;20141207;全文 *
Transdifferentiation And Its Implementation In Autologous Cell Replacement Therapy For Diabetes;Sarah Ferber PhD;《Sheba Medical ctr,Tel Aviv university and Orgenesis Ltd.》;20140529;全文 *

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