CN107674882A - 植物中经空间修饰的基因表达 - Google Patents

植物中经空间修饰的基因表达 Download PDF

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CN107674882A
CN107674882A CN201710806695.6A CN201710806695A CN107674882A CN 107674882 A CN107674882 A CN 107674882A CN 201710806695 A CN201710806695 A CN 201710806695A CN 107674882 A CN107674882 A CN 107674882A
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多米尼克·洛克
亨利克·韦彼·斯盖勒
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Abstract

本发明提供了工程改造植物的方法，所述植物具有基本上集中在植物的木质部组织导管的木质素沉积或木聚糖沉积。本发明也提供了工程改造植物以增加所需的生物合成产物的生产量的方法，例如，以获得增加的次生细胞壁沉积或增加的蜡/角质积累。本发明的经工程改造的植物可用于生物能生产，例如，通过提高源自所述植物的生物质的密度和可消化性和改善水利用要求。

Description

植物中经空间修饰的基因表达
相关申请的交叉引用
本申请要求2011年1月28日提交的美国临时申请号61/437,569的权益，其通过引用并入本文用于所有目的。
关于在联邦资助的研究和开发下做出的发明权的声明
本发明在美国能源部授予的合同号DE-AC02-05CH11231的政府支持下完成。美国政府具有本发明的某些权利。
背景技术
植物细胞壁是造纸工业的唯一纤维素来源，并且是木质纤维素生物燃料的有前途的糖来源。植物将太阳能转化成可运输的和可储存的能量的利用，将对环境具有积极影响，因为使用植物可以帮助急剧减少化石衍生的燃料的利用，可以减少向大气中的碳排放，并且甚至可以促进碳隔离。但是，即使木质纤维素生物燃料对环境有益，生产它们的成本仍然是没有成本效益的，主要是由于源自植物细胞壁的昂贵粗糖。低密度、对酶促水解的不顺从和中等纤维素含量是糖成本的主要促成因素，因为它们会影响运输成本并需要大量能量和化学试剂。因此，提高粗生物质的密度和可消化性将对木质纤维素生物燃料生产成本产生重要的有益影响。
细胞壁不顺从主要由木质素的存在造成，所述木质素嵌入多糖聚合物中，并降低它们的可提取性和水解酶的可达性。植物细胞壁的木质素含量和糖化效率经常高度负相关(Vinzant等人,1997；Chen等人,2007；Jorgensen等人,2007)。不幸的是，大多数降低植物木质素含量的尝试导致了严重的生物质产量下降(Voelker等人,2010；Shadle等人,2007；Franke等人,2002)，并且因此，不容易获得具有显著木质素下降的作物。该细胞壁-生长关系不是木质素独有的；它经常被观察到，并与导管塌缩有关，并且大部分发生在半纤维素或纤维素生物合成所涉及的次生细胞壁基因有缺陷的情况下(Voelker等人,2010；Anterola和Lewis,2002；Brown等人,2005)。这些导管是给地上组织供给通过根系统吸收的水和营养物所必需的(Gomez等人,2008,Boyce等人,2004)。因此，使用沉默策略来减少植物中的木质素，所述沉默策略在酶促步骤抑制水平和生物质产量之间折中。
在木质组织中产生新细胞壁(即所谓的次生细胞壁)，并且所述新细胞壁是当除去水时促成生物质密度的主要组分。优化细胞壁沉积会增加生物质密度并由此增加能量密度。该提高将有益于降低生物质的运输成本，所述运输成本是在生物精炼厂门处递送的生物质价格的重要组分(Searcy等人,2007；Aden等人,2002；Kumar等人,2005)。因此，开发出允许木质组织细胞壁或髓心变厚而不改变植物生长的策略，可以增加生物质和能量密度，并且将有利于木质纤维素生物能生产的成本效益。
另外需要以特定方式工程改造路径中的不同生物合成途径，使得可以在目标组织中靶向生物合成产物的生产。
本发明解决了这些需要。
发明内容
不同的生物学过程存在于从原核生物至真核生物的生物体中，它们由小数目的转录因子调节。在一个方面，本发明提供了一种正反馈回路来增加目标产物在生物体(例如，植物)中的表达。根据本发明的人工正反馈回路(AFPL)采用转录因子/启动子构建体，典型地，其中所述转录因子是调控靶向的生物合成途径的所有或大部分组分的表达的“主要”转录因子。在转录因子诱导或增加基因的表达的情况下，在该途径下游的基因的启动子与编码所述转录因子的核酸可操作地连接，从而导致增加的转录因子表达。AFPL可以用于植物中的任何生物合成方法中，例如，用于控制细胞壁沉积、蜡/角质积累或脂质积累等。
在一个方面，本发明提供了一种工程改造植物以增加生物合成产物在期望的组织中的生产量的方法，所述方法包括:将表达盒引入植物中，其中所述表达盒包含与异源启动子可操作地连接的编码转录因子的多核苷酸，所述转录因子调节生物合成产物的生产，其中所述异源启动子是诱导一定基因的基因表达的启动子，所述一定基因是所述转录因子在期望的组织中的下游靶标；和在表达所述转录因子的条件下，培养所述植物。所述方法可以应用于任何植物，包括单子叶植物和双子叶植物。在某些实施方案中，所述植物是拟南芥属、杨树、桉树、水稻、玉米、柳枝稷、高粱、粟、芒属、甘蔗、松树、苜蓿、小麦、大豆、大麦、草坪草、烟草、大麻、罂粟、竹、油菜、向日葵、柳树或短柄草属。
在某些实施方案中，所述启动子是组织特异性的次生壁启动子，并且所述转录因子会诱导次生壁生物合成产物的表达。例如，所述转录因子可以是NAC次生壁增厚促进因子1(NST1)、NST2、NST3、次生壁相关的NAC结构域蛋白2(SND2)、SND3、MYB结构域蛋白103(MYB103)、MBY85、MYB46、MYB83、MYB58或MYB63。在某些实施方案中，所述组织特异性的次生壁启动子是IRX1、IRX3、IRX5、IRX8、IRX9、IRX14、IRX7、IRX10、GAUT13、GAUT14或CESA4启动子。
在工程改造植物以增加生物合成产物在期望的组织中的生产量的方法的某些实施方案中，所述转录因子会诱导蜡和/或角质的表达。在某些实施方案中，所述转录因子是：shine(SHN)转录因子，其选自SHN1(也被称作WIN1)、SHN2、SHN3、SHN4或SHN5；或MYB 96。在某些实施方案中，所述启动子是CER1、CER2、CER3、CER4、CER5、CER6、CER10、WSD1、Mah1、WBC11、KCS1、KCS2、FATB、LACS1、LACS2、CYP864A、CYP86A7、CYP86A5、KCS10或KCS5启动子。
在另一个方面，本发明提供了一种包含表达盒的植物，所述表达盒包含与异源启动子可操作地连接的编码转录因子的多核苷酸，所述转录因子调节生物合成产物的生产，其中所述异源启动子是诱导一定基因的基因表达的启动子，所述一定基因是所述转录因子在期望的组织中的下游靶标；和在表达所述转录因子的条件下，培养所述植物。所述植物可以是任意植物，包括单子叶植物和双子叶植物。在某些实施方案中，所述植物是拟南芥属、杨树、桉树、水稻、玉米、柳枝稷、高粱、粟、芒属、甘蔗、松树、苜蓿、小麦、大豆、大麦、草坪草、烟草、大麻、罂粟、竹、油菜、向日葵、柳树或短柄草属。
在某些实施方案中，所述植物包含表达构建体，其中启动子是组织特异性的次生壁启动子，且由所述构建体编码的转录因子会诱导次生壁生物合成产物的表达。例如，所述转录因子可以是NAC次生壁增厚促进因子1(NST1)、NST2、NST3、次生壁相关的NAC结构域蛋白2(SND2)、SND3、MYB结构域蛋白103(MYB103)、MBY85、MYB46、MYB83、MYB58或MYB63。在某些实施方案中，所述组织特异性的次生壁启动子是IRX1、IRX3、IRX5、IRX8、IRX9、IRX14、IRX7、IRX10、GAUT13、GAUT14或CESA4启动子。
在某些实施方案中，由所述表达建体编码的转录因子会诱导蜡和/或角质的表达。在某些实施方案中，所述转录因子是：shine(SHN)转录因子，其选自SHN1(也被称作WIN1)、SHN2、SHN3、SHN4或SHN5；或MYB96。在某些实施方案中，所述启动子是CER1、CER2、CER3、CER4、CER5、CER6、CER10、WSD1、Mah1、WBC11、KCS1、KCS2、FATB、LACS1、LACS2、CYP864A、CYP86A7、CYP86A5、KCS10或KCS5启动子。
在一个方面，本发明提供了工程改造植物的方法，所述植物具有基本上集中在植物木质部组织导管的木质素沉积。在某些实施方案中，所述方法包括：
将表达盒引入植物中，其中所述植物被修饰成具有降低的木质素生物合成酶表达水平；且进一步，其中所述表达盒包含与异源的导管特异性的启动子可操作地连接的编码木质素生物合成酶的多核苷酸；和
在表达木质素生物合成酶的条件下，培养所述植物。
在某些实施方案中，所述木质素生物合成酶是PAL、C4H、4CL、HCT、C3H或CCR1。在某些实施方案中，所述木质素生物合成酶是C4H。
在某些实施方案中，所述启动子是VND1、VND2、VND3、VND4、VND5、VND6、VND7、VNI2、REF4或RFR1，例如，与VND1、VND2、VND3、VND4、VND5、VND6、VND7、VNI2、REF4或RFR1启动子基本上相同的启动子；或天然的VND1、VND2、VND3、VND4、VND5、VND6、VND7、VNI2、REF4或RFR1启动子。
在某些实施方案中，如下降低所述经修饰的植物中的木质素生物合成酶的活性水平：使所述植物与反义寡核苷酸接触，所述反义寡核苷酸会沉默编码木质素生物合成酶的基因的表达。在某些实施方案中，所述经修饰的植物(在其中表达与异源启动子可操作地连接的多核苷酸)具有在编码木质素合成酶的基因中的突变，所述突变会减少该酶的表达。
在某些实施方案中，所述植物选自：拟南芥属、杨树、桉树、水稻、玉米、柳枝稷、高粱、粟、芒属、甘蔗、松树、苜蓿、小麦、大豆、大麦、草坪草、烟草、大麻、竹、油菜、向日葵、柳树和短柄草属。
在某些实施方案中，本发明提供了这样的植物、植物细胞、种子、花、叶、果实或生物质：其包含经工程改造成具有基本上集中在植物木质部组织导管的木质素沉积的植物组织。
在另一个方面，本发明提供了在糖化反应中获得增加的量的来自植物的可溶性糖的方法。在某些实施方案中，所述方法包括：使经工程改造成具有基本上集中在植物木质部组织导管的木质素沉积的植物进行糖化反应，由此与野生型植物相比，增加可从植物得到的可溶性糖的量。
在另一个方面，本发明提供了工程改造植物的方法，所述植物具有增加的次生细胞壁沉积。在某些实施方案中，所述方法包括：
将表达盒引入植物中，其中所述表达盒包含与异源启动子可操作地连接的编码转录因子的多核苷酸，所述转录因子调节次生细胞壁在木质组织中的生产量，其中所述启动子与一定基因的天然启动子基本上相同，所述一定基因是所述转录因子的下游靶标；和
在表达所述转录因子的条件下，培养所述植物。在某些实施方案中，所述启动子和所述转录因子、或者所述启动子或所述转录因子来自与在其中建立人工正反馈回路的宿主细胞不同的植物种。在其它实施方案中，所述转录因子和所述启动子来自不同的植物种。
在某些实施方案中，所述转录因子是NST1、NST2、NST3、MYB103、MYB85、MYB46、MYB83、MYB58或MYB63。在某些实施方案中，所述转录因子是NST1。
在某些实施方案中，所述启动子是IRX1、IRX3、IRX5、IRX8、IRX9、IRX14、IRX7或IRX10启动子。在某些实施方案中，所述启动子是天然IRX1、IRX3、IRX5、IRX8、IRX9、IRX14、IRX7或IRX10启动子。
在某些实施方案中，在其中表达与异源启动子可操作地连接的多核苷酸的植物是野生型植物。在某些实施方案中，在其中表达与异源启动子可操作地连接的多核苷酸的植物是具有基本上集中在植物木质部组织导管的木质素沉积的经工程改造的植物。
在某些实施方案中，所述植物选自：拟南芥属、杨树、桉树、水稻、玉米、柳枝稷、高粱、粟、芒属、甘蔗、松树、苜蓿、小麦、大豆、大麦、草坪草、烟草、大麻、竹、油菜、向日葵、柳树和短柄草属。
在某些实施方案中，本发明提供了植物、植物细胞、种子、花、叶、果实或生物质，其包含经工程改造成具有增加的次生细胞壁沉积的植物组织。
在另一个方面，本发明提供了增加从源自植物的生物质实现的生物能生产量的方法。在某些实施方案中，所述方法包括：从经工程改造成具有增加的次生细胞壁沉积的植物收获生物质；和使所述生物质进行转化反应，由此与野生型植物相比增加生物能生产量。
在另一个方面，本发明提供了增加茎/秆/木料强度的方法，所述方法可以减少倒伏和增加来自植物的木材密度。因而，本发明提供了一种在生长过程中增加植物的茎、秆或木料强度的方法，所述方法包括:培养经工程改造成具有增加的次生细胞壁沉积的植物，由此与野生型植物相比提高抗倒伏性。还可以培养具有增加的次生壁沉积的植物，以提供与野生型植物相比具有增加的机械应力抵抗力的植物、或得自这样的植物的生物质。
在另一个方面，本发明提供了工程改造植物的方法，所述植物具有基本上集中在植物木质部组织导管的木聚糖沉积。在某些实施方案中，所述方法包括：
将表达盒引入植物中，其中所述植物被修饰成具有降低的木聚糖生物合成酶活性水平；且进一步，其中所述表达盒包含与异源的导管特异性的启动子可操作地连接的编码木聚糖生物合成酶的多核苷酸；和
在表达所述木聚糖生物合成酶的条件下，培养所述植物。在某些实施方案中，在其中引入表达盒的植物被修饰成具有降低的木聚糖生物合成酶表达水平。
在某些实施方案中，所述木聚糖生物合成酶是不规则的木质部8(IRX8)、IRX14、IRX14-like、IRX9、IRX9-like、IRX7、IRX10、IRX10-like、IRX15、IRX15-like、F8H或PARVUS。
在某些实施方案中，所述启动子是VND1、VND2、VND3、VND4、VND5、VND6、VND7、VNI2、REF4或RFR1，例如，与VND1、VND2、VND3、VND4、VND5、VND6、VND7、VNI2、REF4或RFR1启动子基本上相同的启动子；或天然的VND1、VND2、VND3、VND4、VND5、VND6、VND7、VNI2、REF4或RFR1启动子。
在某些实施方案中，如下降低经修饰的植物中的木聚糖生物合成酶的活性水平：使所述植物与反义寡核苷酸接触，所述反义寡核苷酸会沉默编码木聚糖生物合成酶的基因的表达。在某些实施方案中，所述经修饰的植物(在其中表达与异源启动子可操作地连接的多核苷酸)具有在编码木聚糖合成酶的基因中的突变，所述突变会减少该酶的表达。在某些实施方案中，如下降低经修饰的植物中的木聚糖生物合成酶的活性：使所述植物与突变的木聚糖生物合成基因接触，所述基因编码具有显性阴性突变的蛋白并造成木聚糖生物合成的减少。
在某些实施方案中，所述植物选自：拟南芥属、杨树、桉树、水稻、玉米、柳枝稷、高粱、粟、芒属、甘蔗、松树、苜蓿、小麦、大豆、大麦、草坪草、烟草、大麻、竹、油菜、向日葵、柳树和短柄草属。
在某些实施方案中，本发明提供了植物、植物细胞、种子、花、叶、果实或生物质，其包含经工程改造成具有基本上集中在植物木质部组织导管的木聚糖沉积的植物组织。
在另一个方面，本发明提供了在糖化反应中获得增加的量的来自植物的可溶性糖的方法。在某些实施方案中，所述方法包括：使经工程改造成具有基本上集中在植物木质部组织导管的木聚糖沉积的植物进行糖化反应，由此与野生型植物相比，增加可从植物得到的可溶性糖的量。
在另一个方面，本发明提供了工程改造植物的方法，所述植物具有基本上集中在植物木质部组织导管的木聚糖O-乙酰化。在某些实施方案中，所述方法包括：
将表达盒引入植物中，其中所述植物被修饰成具有降低的负责木聚糖O-乙酰化的酶的表达水平；且进一步，其中所述表达盒包含与异源的导管特异性的启动子可操作地连接的编码木聚糖O-乙酰化酶的多核苷酸；和
在表达木聚糖O-乙酰化酶的条件下，培养所述植物。
在某些实施方案中，所述木聚糖O-乙酰化酶是RWA蛋白。
在某些实施方案中，所述木聚糖O-乙酰化酶是毛状体双折射样(TrichomeBirefringence Like)蛋白家族(PF03005家族也被称作未知功能结构域(Domain ofUnknown Function)231)的成员。
在某些实施方案中，所述启动子是VND1、VND2、VND3、VND4、VND5、VND6、VND7、VNI2、REF4或RFR1，例如，与VND1、VND2、VND3、VND4、VND5、VND6、VND7、VNI2、REF4或RFR1启动子基本上相同的启动子；或天然的VND1、VND2、VND3、VND4、VND5、VND6、VND7、VNI2、REF4或RFR1启动子。
在某些实施方案中，如下降低经修饰的植物中的木聚糖O-乙酰化酶的表达水平：使所述植物与反义寡核苷酸接触，所述反义寡核苷酸会沉默编码木聚糖O-乙酰化酶的基因的表达。在某些实施方案中，所述经修饰的植物(在其中表达与异源启动子可操作地连接的多核苷酸)具有在编码木聚糖O-乙酰化酶的基因中的突变，所述突变会减少该酶的表达。
在某些实施方案中，所述植物选自：拟南芥属、杨树、桉树、水稻、玉米、柳枝稷、高粱、粟、芒属、甘蔗、松树、苜蓿、小麦、大豆、大麦、草坪草、烟草、大麻、竹、油菜、向日葵、柳树和短柄草属。
在某些实施方案中，本发明提供了植物、植物细胞、种子、花、叶、果实或生物质，其包含经工程改造成具有基本上集中在植物木质部组织导管的木聚糖沉积的植物组织。
在另一个方面，本发明提供了在糖化反应中获得增加的量的来自植物的可溶性糖的方法。在某些实施方案中，所述方法包括：使经工程改造成具有基本上集中在植物木质部组织导管的木聚糖O-乙酰化的植物进行糖化反应，由此与野生型植物相比，增加可从植物得到的可溶性糖的量。
附图说明
图1.苯丙氨酸氨裂合酶(PAL)比对。使用ClustalW，比对了得自下述植物的PAL的蛋白序列：拟南芥(Arabidopsis thaliana)(“AtPAL1”(SEQ ID NO:2))、小立碗藓(Physcomitrella patens)(藓)(“PpPAL3”(SEQ ID NO:97))、水稻(Oryza sativa)(稻)(“OsPAL”(SEQ ID NO:98))、玉米(Zea mays)(玉米)(“ZmPAL”(SEQ ID NO:99))、两色高梁(Sorghum bicolor)(高粱)(“SbPAL”(SEQ ID NO:100))、马尾松(Pinus massoniana)(松树)(“PlPAL”(SEQ ID NO:101))、紫苜蓿(苜缩属sativa)(苜蓿)(“MsPAL”(SEQ ID NO:102))、小麦(Triticum aestivum)(小麦)(“TaPAL”(SEQ ID NO:103))、大豆(Glycine max)(大豆)(“GmPAL2”(SEQ ID NO:104))、饲用甜菜(Beta vulgaris)(糖用甜菜)(“BvPAL”(SEQID NO:105))、红花烟草(Nicotiniana tabacum)(烟草)(“NtPAL1”(SEQ ID NO:106))、马铃薯(Solanum tuberosum)(马铃薯)(“StPAL1”(SEQ ID NO:107))、绿竹(Bambusa oldhamii)(竹)(“BoPAL”(SEQ ID NO:108))、芫菁(Brassica rapa)(“BnPAL1”(SEQ ID NO:109))、向日葵(Helianthus annuus)(向日葵)(“HaPAL”(SEQ ID NO:110))、蓖麻(Ricinuscommunis)(“RcPAL”(SEQ ID NO:111))、葡萄(Vitis vinifera)(葡萄)(“VvPAL”(SEQ IDNO:112))、麻疯树(麻风树属curcas)(“JcPAL”(SEQ ID NO:113))、一品红(Euphorbiapulcherrima)(一品红)(“EpPAL”(SEQ ID NO:114))、红车轴草(Trifolium pratense)(三叶草)(“TpPAL”(SEQ ID NO:115))、日本百脉根(Lotus japonicus)(“LjPAL5”(SEQ ID NO:116))和江南卷柏(Selaginella moellendorffii)(卷柏)(“SmPAL”(SEQ ID NO:117))。大多数(共有)＝SEQ ID NO:96。
图2.肉桂酸4-羟化酶(C4H)比对。使用ClustalW，比对了得自下述植物的C4H的蛋白序列：拟南芥(“AtC4H”(SEQ ID NO:4))、火炬松(Pinus taeda)(松树)(“PtC4H”(SEQ IDNO:119))、水稻(稻)(“OsC4H”(SEQ ID NO:120))、玉米(玉米)(“ZmC4H”(SEQ ID NO:121))、两色高梁(高粱)(“SbC4H”(SEQ ID NO:122))、蒺藜状苜蓿(苜缩属truncatula)(“MtC4H”(SEQ ID NO:123))、小麦(小麦)(“TaC4H”(SEQ ID NO:124))、大豆(大豆)(“GmC4H”(SEQ IDNO:125))、红花烟草(烟草)(“NtC4H”(SEQ ID NO:126))、马铃薯(马铃薯)(“StC4H”(SEQ IDNO:127))、绿竹(竹)(“BoC4H”(SEQ ID NO:128))、甘蓝型油菜(Brassica napus)(“BnC4H1”(SEQ ID NO:129))、向日葵(向日葵)(“HaC4H”(SEQ ID NO:130))、蓖麻(“RcC4H”(SEQ IDNO:131))、葡萄(葡萄)(“VvC4H”(SEQ ID NO:132))、一品红(一品红)(“EpC4H”(SEQ ID NO:133))、红车轴草(三叶草)(“TpC4H”(SEQ ID NO:134))和江南卷柏(卷柏)(“SmC4H”(SEQ IDNO:135))。大多数(共有)＝SEQ ID NO:118。
图3.4-香豆酸-CoA连接酶(4CL)比对。使用ClustalW，比对了得自下述植物的4CL的蛋白序列：拟南芥(“At4CL2”(SEQ ID NO:6)和“At4CL1”(SEQ ID NO:137))、红花烟草(烟草)(“Nt4CL1”(SEQ ID NO:138)和“Nt4CL2”(SEQ ID NO:144))、赤桉(Eucalyptuscamaldulensis)(“Ec4CL”(SEQ ID NO:139)、“Ec4CL1”(SEQ ID NO:142)和“Ec4CL2”(SEQID NO:143))、火炬松(松树)(“Pt4CL”(SEQ ID NO:145)和“Pt4CL1”(SEQ ID NO:140))、大豆(大豆)(“Gm4CL1”(SEQ ID NO:141))、水稻(稻)(“Os4CL3”(SEQ ID NO:146)和“Os4CL4”(SEQ ID NO:150))、两色高梁(高粱)(“Sb4CL”(SEQ ID NO:147))、玉米(玉米)(“Zm4CL”(SEQ ID NO:148))、柳枝稷(柳枝稷)(“Pv4CL”(SEQ ID NO:149))、黑麦草(Loliumperenne)(黑麦草)(“Lp4CL3”(SEQ ID NO:151))、江南卷柏(卷柏)(“Sm4CL1”(SEQ ID NO:152))和小立碗藓(藓)(“Pp4CL1”(SEQ ID NO:153))。大多数(共有)＝SEQ ID NO:136。
图4.羟基肉桂酰辅酶A:莽草酸羟基肉桂酰基转移酶(HCT)比对。使用ClustalW，比对了得自下述植物的HCT的蛋白序列：拟南芥(“AtHCT”(SEQ ID NO:8))、琴叶拟南芥(Arabidopsis lyrata)(“AlHCT”(SEQ ID NO:155))、火炬松(松树)(“PtHCT”(SEQ ID NO:156))、蓖麻(“RcHCT”(SEQ ID NO:157))、中果咖啡(Coffea canephora)(“CcHCT”(SEQ IDNOS:158和162))、葡萄(葡萄)(“VvHCT”(SEQ ID NO:159))、红花烟草(烟草)(“NtHCT”(SEQID NO:160))、红车轴草(三叶草)(“TpHCT”(SEQ ID NO:161))、水稻(稻)(“OsHCT”(SEQ IDNO:163)和“OsHCT3”(SEQ ID NO:164))、两色高梁(高粱)(“SbHCT”(SEQ ID NO:165))、玉米(玉米)(“ZmHCT”(SEQ ID NO:166)和“ZmHCT2”(SEQ ID NO:167))、燕麦(燕麦)(“AsHCT”(SEQ ID NO:168))和江南卷柏(卷柏)(“SmHCT1”(SEQ ID NO:169)和“SmHCT2”(SEQ ID NO:170))。大多数(共有)＝SEQ ID NO:154。
图5.香豆酰基莽草酸3-羟化酶(C3H)比对。使用ClustalW，比对了得自下述植物的C3H的蛋白序列：拟南芥(“AtC3H”(SEQ ID NO:10))、蓝桉(Eucalyptus globulus)(“EgC3H”(SEQ ID NO:172))、蓖麻(“RcC3H”(SEQ ID NO:173))、葡萄(葡萄)(“VvC3H”(SEQ ID NO:174))、大豆(大豆)(“GmC3H”(SEQ ID NO:175))、红车轴草(三叶草)(“TpC3H”(SEQ ID NO:176))、蒺藜状苜蓿(“MtC3H”(SEQ ID NO:177))、中果咖啡(“CcC3H”(SEQ ID NO:178))、罗勒(罗勒)(“ObC3H”(SEQ ID NO:179))、火炬松(松树)(“PtC3H”(SEQ ID NOS:180和181))、红花烟草(烟草)(“NtC3H”(SEQ ID NO:182))、银杏树(Ginkgo biloba)(“GbC3H”(SEQ IDNO:183))、两色高梁(高粱)(“SbC3H”(SEQ ID NO:184))、玉米(玉米)(“ZmC3H”(SEQ ID NO:185))、水稻(稻)(“OsC3H”(SEQ ID NOS:186和188))、小麦(小麦)(“TaC3H”(SEQ ID NO:187))、江南卷柏(卷柏)(“SmC3H”(SEQ ID NO:189))和小立碗藓(藓)(“FpC3H”(SEQ ID NO:190))。大多数(共有)＝SEQ ID NO:171。
图6.肉桂酰基辅酶A还原酶(CCR)比对。使用ClustalW，比对了得自下述植物的CCR的蛋白序列：拟南芥(“AtCCR1”(SEQ ID NO:12))、番茄(Solanum lycopersicum)(番茄)(“SlCCR”(SEQ ID NO:192))、一品红(一品红)(“EpCCR”(SEQ ID NO:193))、马铃薯(马铃薯)(“StCCR”(SEQ ID NO:194))、冈尼桉(Eucalyptus gunnii)(“EgCCR”(SEQ ID NO:195))、葡萄(葡萄)(“VvCCR”(SEQ ID NO:196))、蓖麻(“RcCCR”(SEQ ID NO:197))、火炬松(松树)(“PtCCR”(SEQ ID NOS:198和199))、大豆(大豆)(“GmCCR”(SEQ ID NO:200))、挪威云杉(Picea abies)(云杉)(“PaCCR”(SEQ ID NO:201))、马尾松(松树)(“PmCCR”(SEQ IDNO:202))、水稻(稻)(“OsCCR”(SEQ ID NO:203))、黑麦草(黑麦草)(“LpCCR”(SEQ ID NO:204))、柳枝稷(柳枝稷)(“PvCCR”(SEQ ID NOS:205和207))、两色高梁(高粱)(“SbCCR”(SEQID NO:206))、甘蔗(Saccharum officiunarum)(甘蔗)(“SoCCR”(SEQ ID NO:208))、大麦(Hordeum vulgare)(大麦)(“HvCCR”(SEQ ID NO:209))、玉米(玉米)(“ZmCCR”(SEQ ID NO:210))和江南卷柏(卷柏)(“SmCCR”(SEQ ID NO:211))。大多数(共有)＝SEQ ID NO:191。
图7.IRX8序列比对。拟南芥属IRX8(GAUT12)和同源蛋白的氨基酸序列比对。用COBALT(Papadopoulos JS和Agarwala R(2007)COBALT：constraint-based alignmenttool for multiple protein sequences，Bioinformatics 23:1073-79)进行所述比对。通过它们的GenBank蛋白ID来鉴别蛋白。gi15239707：得自拟南芥的IRX8(SEQ ID NO:212)；gi2241262287：得自毛果杨(Populus trichocarpa)的同系物(SEQ ID NO:213)；gi224117396：得自毛果杨的同系物(SEQ ID NO:214)；gi224141469：得自毛果杨的同系物(SEQ ID NO:215)；gi224077712：得自毛果杨的同系物(SEQ ID NO:216)；gi302803855：得自江南卷柏的同系物(SEQ ID NO:217)；gi30678270：得自拟南芥的GAUT13(SEQ ID NO:218)；gi30685369：得自拟南芥的GAUT14(SEQ ID NO:219)；gi115489272：得自水稻的同系物(SEQ ID NO:220)；gi224131384：得自毛果杨的同系物(SEQ ID NO:221)；gi22331857：得自拟南芥的GAUT15(SEQ ID NO:222)。
图8.IRX14比对。拟南芥属IRX14和同源蛋白的氨基酸序列比对。用COBALT(Papadopoulos JS和Agarwala R(2007)COBALT：constraint-based alignment tool formultiple protein sequences，Bioinformatics 23:1073-79)进行所述比对。通过它们的GenBank蛋白ID来鉴别蛋白。gi|30690793：得自拟南芥的IRX14(SEQ ID NO:223)；gi|15240245：得自拟南芥的IRX14-like(SEQ ID NO:224)；gi|224096716和gi|224081752：得自毛果杨的同系物(SEQ ID NOS:225和226)；gi|302797519：得自江南卷柏的同系物(SEQID NO:227)；gi|115469624：得自水稻的同系物(SEQ ID NO:228)。
图9.IRX9比对。拟南芥属IRX9和同源蛋白的氨基酸序列比对。用COBALT(Papadopoulos JS和Agarwala R(2007)COBALT：constraint-based alignment tool formultiple protein sequences，Bioinformatics 23:1073-79)进行所述比对。通过它们的GenBank蛋白ID来鉴别蛋白。gi|15228084：得自拟南芥的IRX9(SEQ ID NO:229)；gi|224140167和gi|224069352：得自毛果杨的同系物(SEQ ID NOS:230和231)；gi|297600755和gi|115461821：得自水稻的同系物(SEQ ID NOS:232和233)；gi|224092304：得自毛果杨的同系物(SEQ ID NO:234)；gi|302759368：得自江南卷柏的同系物(SEQ ID NO:235)；gi|42571663：得自拟南芥的IRX9-like(SEQ ID NO:236)；gi|224063335：得自毛果杨的同系物(SEQ ID NO:237)；gi|115439133，gi|115474279，gi|115465403，gi|115481434和gi|115456794：得自水稻的同系物(SEQ ID NOS:238-242)。
图10.IRX7比对。拟南芥属IRX7(FRA8)和同源蛋白的氨基酸序列比对。用COBALT(Papadopoulos JS和Agarwala R(2007)COBALT：constraint-based alignment tool formultiple protein sequences，Bioinformatics 23:1073-79)进行所述比对。通过它们的GenBank蛋白ID来鉴别蛋白。gi|42570324：得自拟南芥的IRX7(SEQ ID NO:243)；gi|224106838：得自毛果杨的同系物(SEQ ID NO:244)；gi|42568020：得自拟南芥的IRX7-like(F8H)(SEQ ID NO:245)；gi|115450193：得自水稻的同系物(SEQ ID NO:246)；gi|302786830和gi|302826405：得自江南卷柏的同系物(SEQ ID NOS:247和248)。
图11.IRX10比对。拟南芥属IRX10和同源蛋白的氨基酸序列比对。用COBALT(Papadopoulos JS和Agarwala R(2007)COBALT：constraint-based alignment tool formultiple protein sequences，Bioinformatics 23:1073-79)进行所述比对。通过它们的GenBank蛋白ID来鉴别蛋白。gi|18424516：得自拟南芥的IRX10-like(GUT1)(SEQ ID NO:249)；gi|224119858：得自毛果杨的同系物(SEQ ID NO:250)；gi|15223522：得自拟南芥的IRX10(GUT2)(SEQ ID NO:251)；gi|224053575和gi|224075447：得自毛果杨的同系物(SEQID NOS:252和253)；gi|115441967：得自水稻的Os01g0926600(SEQ ID NO:254)；gi|302783378：得自江南卷柏的GT47D1(SEQ ID NO:255)；gi|115458146：得自水稻的Os04g0398600(SEQ ID NO:256)；gi|115441965：得自水稻的Os01g0926400(SEQ ID NO:257)；gi|115481310：得自水稻的Os10g0180000(SEQ ID NO:258)；gi|224106838：得自毛果杨的同系物(SEQ ID NO:259)。
图12。Parvus序列比对。拟南芥属PARVUS(GATL1)和同源蛋白的氨基酸序列比对。用COBALT(Papadopoulos JS和Agarwala R(2007)COBALT：constraint-based alignmenttool for multiple protein sequences，Bioinformatics 23:1073-79)进行所述比对。通过它们的GenBank蛋白ID来鉴别蛋白。gi|18394719：得自拟南芥的PARVUS(SEQ ID NO:260)。其它蛋白是得自拟南芥(SEQ ID NOS:265、269-273和275-277)、毛果杨(SEQ ID NOS:261-264、266和267)和水稻(SEQ ID NOS:268、274和278-280)的一些同系物，和得自江南卷柏的单一同系物(gi|302807664)(SEQ ID NO:281)。
图13.NAC次生壁增厚促进因子(NST)比对。使用ClustalW，比对了得自下述植物的NST的蛋白序列：拟南芥(“AtNST1”(SEQ ID NO:14)、“AtNST2”(SEQ ID NO:283)和“SND1”(SEQ ID NO:284))、火炬松(松树)(“PtNAC023”(SEQ ID NO:285)、“PtNAC065”(SEQ ID NO:288)和“PtNAC”(SEQ ID NOS:296和297))、蒺藜状苜蓿(“MtNAC1”(SEQ ID NO:286))、大豆(大豆)(“GmNAM1”(SEQ ID NO:287))、葡萄(葡萄)(“VvNST”(SEQ ID NO:289))、蓖麻(“RcNST”(SEQ ID NO:290))、冈尼桉(“EgNST”(SEQ ID NO:291))、玉米(玉米)(“ZmNST”(SEQ ID NO:292))、两色高梁(高粱)(“SbNST”(SEQ ID NO:293、295和298))、水稻(稻)(“OsNAC7”(SEQ ID NOS:294和302)和“OsNST”(SEQ ID NO:301))、西加云杉(Piceasitchensis)(云杉)(“PsNST”(SEQ ID NO:299))、苹果(“AppleT”(SEQ ID NO:300))和江南卷柏(卷柏)(“SmNST1”)(SEQ ID NO:303)。大多数(共有)＝SEQ ID NO:282。
图14.调节次生细胞壁生物合成的转录网络。呈现了调节导管元件和纤维中的次生细胞壁沉积的主要转录因子，以及在次生细胞壁生物合成过程中诱导的几个下游靶基因。呈现的转录因子能够诱导在纤维素、半纤维素和/或木质素生物合成中涉及的基因的表达。该图取自Zhong等人,2007。
图15.经工程改造的植物系的细胞壁的木质素分析。A.使用乙酰基溴方法对得自野生型(W)和经工程改造的(“Eng Lig I”)(ref3-2+pVND6:C4H)植物的衰老茎的木质素定量。B.从左至右分别是同龄野生型(W)和2种经工程改造的Eng Lig I植物的、用间苯三酚染色的茎横截面的亮光图像。
图16.Eng Lig I系的分析。A.在2个不同的生长阶段对比了Eng Lig I的植物生长表型。上图描绘了营养体阶段，下图描绘了成熟体阶段(抽苔阶段)。在A-D中，野生型植物显示在左侧，经工程改造的Eng Lig I植物显示在右侧。B.从干燥茎释放的糖，所述干燥茎用NaOH预处理并与纤维素酶混合液一起温育0、24或48小时。C.从干燥茎释放的糖，所述干燥茎用热水预处理并与纤维素酶混合液一起温育0、24或48小时。D.从干燥茎释放的糖，所述干燥茎用稀酸预处理并与纤维素酶混合液一起温育0、24或48小时。
图17.Eng Lig II系的分析。A.在2个不同的生长阶段对比了Eng Lig II(ref3-2+pVND6:C4H+pIRX8:NST1)的植物生长表型。上图描绘了营养体阶段，下图描绘了成熟体阶段(抽苔阶段)。野生型植物显示在左侧，经工程改造的Eng Lig II植物显示在右侧。B.从左至右分别是同龄野生型(W)、ref3-2突变体和经工程改造的Eng Lig II植物的、用间苯三酚染色的茎横截面的亮光图像。C.使用乙酰基溴方法对得自野生型(W)、经工程改造的Eng LigI和经工程改造的Eng Lig II植物的衰老茎的木质素定量。
图18.穿过野生型(A,C)和经工程改造的(ref3-2+pVND6:C4H+pIRX8:NST1)(B,D)植物的横截面的透射电子显微照片。A-B.植物的木质部组织。C-D.植物的维管束间组织。“Ve,”“Xf,”和“If”分别代表导管、木质纤维和维管束间纤维。
图19.Eng Lig I和Eng Lig II系的糖化效率。A.从干燥茎释放的糖，所述干燥茎用热水预处理并与纤维素酶混合液一起温育0-144小时。得自野生型(野生型；蓝色)植物、经工程改造的Eng Lig I(橙色)植物或Eng Lig II(红色)植物的茎。B.从干燥茎释放的糖，所述干燥茎用NaOH预处理并与纤维素酶混合液一起温育0-144小时。得自野生型(野生型；蓝色)植物、经工程改造的Eng Lig I(橙色)植物或Eng Lig II(红色)植物的茎。
图20.启动子活性表征。A.从左至右分别是得自野生型(WT)、cadc/d突变体、用pVND6:CADc转化的cadc/d突变体和用pC4H:CADc转化的cadc/d突变体的5-10cm茎的基部的茎横截面的明视野图像。由于CAD活性缺乏而产生红色。B.从左至右分别是得自野生型(WT)、f5h突变体、用pVND6:F5H转化的f5h突变体和用pC4H:F5H转化的f5h突变体的5-10cm茎的基部的Maule染色的茎横截面的明视野图像。由于芥子醇的存在而产生红色，并且所述红色代表在Maule染色反应过程中发生反应的木质素中的芥子醇的量。通过天然F5H基因的表达，恢复了f5h突变体中的芥子醇的生产。
图21.木质部塌缩。A.同龄成体ref3-2突变体(纯合子c4h突变体)和野生型植物(wt)(分别为右图和左图)。B.相同生长龄ref3-2突变体(纯合子c4h突变体)和野生型植物(分别为右图和左图)。C.上图和下图分别描绘了放大20和40倍的间苯三酚染色的茎横截面的明视野图像，所述茎得自在与A所示相同龄取样的野生型和ref3-2(分别为左图和右图)。黄色箭头指向ref3-2突变体中的一些塌缩导管。
图22.NST1的表达分析。通过半定量RT-PCR分析了NST1表达。pIRX8:NST1：使用特异性的NST1引物来证实由pIRX8启动子驱动的NST1的表达。NST1：使用特异性的NST1引物来证实各自由pIRX8和pNST1启动子驱动的2个NST1基因的表达。pVND6:C4H:使用特异性的C4H引物来证实由pVND6驱动的C4H基因的表达。C4H:使用特异性的C4H引物来证实由pVND6或pC4H启动子驱动的C4H基因(野生型和ref3-2突变体等位基因)的表达。微管蛋白：使用特异性的微管蛋白引物来证实用于RT-PCR的RNA的质量和数量。泳道1-4显示了独立的Eng LigII(ref3-2+pVND6:C4H+pIRX8:NST1)植物；泳道5显示了一种野生型植物；泳道6和7显示了独立的Eng Lig I(ref3-2+pVND6:C4H)植物；且泳道8显示了一种ref3-2突变体植物。
图23.细胞壁厚度。A-D.在得自Col0(WT)(A)、ref3-2(c4h突变体)(B)、Eng Lig I(C)和Eng Lig II(D)植物中的维管束内区域的20个独立纤维细胞上测量的细胞壁厚度和细胞直径。通过将细胞壁厚度总和(μm)除以细胞直径(μm)来测量细胞壁比率。E.细胞壁厚度和细胞直径测量方法。绿色条(a)和黄色条(b)各自代表细胞壁厚度测量结果，且粉红色条代表细胞直径。通过将细胞壁厚度总和(μm)除以细胞直径(μm)来测量细胞壁比率：(a+b)/细胞直径。
图24.化学水解后从细胞壁释放的糖。A-B.TFA水解后的半纤维素组成。A.释放的主要糖的定量(mg糖/mg干燥细胞壁)。B.每种糖在释放的总量中的百分比。C.在H2SO4水解后释放的总糖。
图25.SHN蛋白序列的比对。使用ClustalW，比对了得自下述植物的SHN多肽的蛋白序列：拟南芥(“At”(SEQ ID NOS:37、305和306))、毛果杨(“Pt”(SEQ ID NOS:307-311))、蒺藜状苜蓿(“Mt”(SEQ ID NO:312-316))、水稻(“Os”(SEQ ID NO:317))、紫短柄草(Brachypodium distachyon)(“Bd”(SEQ ID NOS:318和319))、玉米(“Zm”(SEQ ID NO:320))、两色高梁(“Sb”(SEQ ID NOS:321和322))、大麦(“Hv”(SEQ ID NO:323))、西加云杉(“Ps”(SEQ ID NO:324))、江南卷柏(“Sm”(SEQ ID NO:325))和小立碗藓(“Pp(SEQ ID NO:326))。大多数(共有)＝SEQ ID NO:304。
图26.Myb96蛋白序列的比对。使用ClustalW，比对了得自下述植物的Myb96多肽的蛋白序列：拟南芥(“At”(SEQ ID NOS:80和81))、小盐芥(Thellungiella halophila)(“Th”(SEQ ID NO:82))、蒺藜状苜蓿(“Mt”(SEQ ID NOS:85和86))、毛果杨(“Pt”(SEQ ID NO:84))、葡萄(“Vv”(SEQ ID NO:83))、大叶来檬(Citrus macrophylla)(“Cm”(SEQ ID NO:87))、紫短柄草(“Bd”(SEQ ID NOS:88和89))、小麦(“Ta”(SEQ ID NO:90))、水稻(“Os”(SEQID NOS:91和92))和玉米(“Zm”(SEQ ID NO:93))。大多数(共有)＝SEQ ID NO:327。
图27.细胞壁人工正反馈回路的表示。图27描绘了一种示例性的细胞壁致密化策略。
图28.靶组织中的蜡生物合成途径的诱导。图28描绘了用于诱导靶组织中的蜡生物合成途径的示例性人工正反馈回路。
图29.经工程改造的细胞壁植物系的植物生长表型。野生型、c4h突变体植物和经工程改造的植物系的生长对比，在所述经工程改造的植物系中，ref3-2突变被pREF4:C4H(A)或pRFR1:C4H(B)DNA构建体补充。
图30.经工程改造的细胞壁植物系的木质素分布和含量。木质素分布显示在上图中。木质素定量显示在下图中。
图31.木质素工程改造的植物系的糖化效率。图A和B显示了从干燥茎释放的糖，所述茎使用热水(图A)或碱(图B)预处理，随后与纤维素酶混合液一起温育。图C提供了糖化结果的总结。
图32.细胞壁致密化反馈回路。图A解释了含有DNA构建体pCesA4:NST1的拟南芥属野生型植物中的细胞壁致密化。图B显示了使用pAtlRX8:AtNST1DNA构建体对短柄草属野生型植物细胞壁的致密化，其中所述启动子和转录因子都得自拟南芥属。
图33.木聚糖工程改造化的例子。野生型、突变体和用由pVND6或pVND7驱动的突变的IRX7、IRX8或IRX9基因的野生型形式补充的突变体植物的生长对比。
图34.转化体的后代的生长。通过用pVND7:IRX7表达构建体转化irx7突变体制备的4个单个转化体的后代的生长。
图35.转化体的后代的生长。通过用pVND7:IRX9表达构建体转化irx9突变体制备的2个单个转化体的后代的生长。
图36.从转化体制备的非纤维质单糖组成。从4个单个转化体制备的细胞壁的非纤维质单糖组成，所述转化体通过用pVND7:IRX7表达构建体转化irx7突变体来制备。
图37.从转化体制备的非纤维质单糖组成。从4个单个转化体制备的细胞壁的非纤维质单糖组成，所述转化体通过用pVND6:IRX8表达构建体转化irx8突变体来制备。
图38.从单个转化体制备的茎细胞壁的非纤维质单糖组成。从4个单个转化体的后代制备的茎细胞壁的非纤维质单糖组成，所述转化体通过用pVND7:IRX9表达构建体转化irx9突变体来制备。
图39.细胞壁的糖化分析。从2个单个转化体的后代制备的细胞壁的糖化分析，所述转化体通过用pVND6:IRX9表达构建体转化irx9突变体来制备。
图40.被转化以建立人工正反馈回路的植物中的蜡沉积。用不同构建体转化的拟南芥属植物的视觉分析表明了与对照植物相比增加的叶片亮度。
具体实施方式
I.定义
本文中使用的术语“木质素生物合成酶”表示，调节植物中的木质素单体(对-香豆酰基(4-羟基肉桂基)醇、松柏基(3-甲氧基4-羟基肉桂基)醇、和芥子基(3,5-二甲氧基4-羟基肉桂基)醇)的合成的蛋白。该术语包括本文所述的具体酶的多态变体、等位基因、突变体和种间同系物。编码木质素生物合成酶的核酸表示基因、前-mRNA、mRNA等，包括编码本文所述的具体序列的多态变体、等位基因、突变体和种间同系物的核酸。因而，在某些实施方案中，木质素生物合成核酸(1)具有这样的核酸序列：其与SEQ ID NO:1、3、5、7、9或11中的任一个的核酸序列具有大于约50％核苷酸序列同一性、55％、60％、65％、70％、75％、80％、85％、90％、优选地91％、92％、93％、94％、95％、96％、97％、98％或99％或更高的核苷酸序列同一性，优选地在至少约10个、15个、20个、25个、50个、100个、200个、500个或更多个核苷酸的区域内或在整个多核苷酸的长度内；或(2)编码这样的多肽：所述多肽的氨基酸序列与由SEQ ID NO:1、3、5、7、9或11中的任一个的核酸序列编码的多肽、或与SEQ ID NO:2、4、6、8、10或12中的任一个的氨基酸序列、或与图1-6中的任一个所示的任意序列具有大于约50％氨基酸序列同一性、55％、60％、65％、70％、75％、80％、85％、90％、优选地91％、92％、93％、94％、95％、96％、97％、98％或99％或更大的氨基酸序列同一性，优选地在至少约25个、50个、100个、200个或更多个氨基酸的区域内或在整个多肽的长度内。在某些实施方案中，木质素生物合成酶或木质素生物合成多肽具有这样的氨基酸序列：其与SEQ ID NO:2、4、6、8、10或12中的任一个的氨基酸序列、或与图1-6中的任一个所示的任意氨基酸序列具有大于约50％氨基酸序列同一性、55％、60％、65％、70％、75％、80％、85％、90％、优选地91％、92％、93％、94％、95％、96％、97％、98％或99％或更大的氨基酸序列同一性，优选地在至少约25个、50个、100个、200个或更多个氨基酸的区域内或在整个多肽的长度内。
通过名称(例如，肉桂酸4-羟化酶)、基因符号(例如，C4H)或登录号(例如，NM_128601(对于核酸)或NP_180607(对于蛋白))，可以鉴别木质素生物合成酶。应当理解，所有这些标识符表示相同的生物标记，因而是等效的。在某些实施方案中，所述木质素生物合成酶是苯丙氨酸氨裂合酶(PAL)(登录号NM_129260或NP_181241)、肉桂酸4-羟化酶(C4H)(登录号NM_128601或NP_180607)、4-香豆酸-CoA连接酶(4CL)(登录号NM_113019或NP_188761)、羟基肉桂酰辅酶A:莽草酸羟基肉桂酰基转移酶(HCT)(登录号NM_124270或NP_199704)、香豆酰基莽草酸3-羟化酶(C3H)(登录号NM_119566或NP_850337)或肉桂酰基辅酶A还原酶1(CCR1)(登录号NM_101463或NP_173047)。
本文中使用的术语“木聚糖生物合成酶”表示在木聚糖合成中涉及的酶。本文中使用的该术语还可以表示修饰木聚糖的酶，例如，乙酰化木聚糖的酶。该术语包括本文所述的具体多肽的多态变体、等位基因、突变体和种间同系物。编码木聚糖生物合成酶的核酸表示基因、前-mRNA、mRNA等，包括编码本文所述的具体氨基酸序列的多态变体、等位基因、突变体和种间同系物的核酸。因而，在某些实施方案中，木聚糖生物合成酶编码这样的多肽：所述多肽的氨基酸序列与图7-12中的任一个所示的任意序列具有大于约50％氨基酸序列同一性、55％、60％、65％、70％、75％、80％、85％、90％、优选地91％、92％、93％、94％、95％、96％、97％、98％或99％或更大的氨基酸序列同一性，优选地在至少约25个、50个、100个、200个或更多个氨基酸的区域内或在整个多肽的长度内。在图7-12中提供的登录号下可得到木聚糖生物合成酶的例子的核酸序列。在某些实施方案中，木聚糖生物合成酶具有这样的氨基酸序列：其与图7-12中的任一个所示的任意序列具有大于约50％氨基酸序列同一性、55％、60％、65％、70％、75％、80％、85％、90％、优选地91％、92％、93％、94％、95％、96％、97％、98％或99％或更大的氨基酸序列同一性，优选地在至少约25个、50个、100个、200个或更多个氨基酸的区域内或在整个多肽的长度内。在某些实施方案中，所述木聚糖生物合成酶是不规则的木质部8(IRX8)、IRX14、IRX14-like、IRX9、IRX9-like、IRX7、IRX10、IRX10-like、F8H、PARVUS或RWA1、RWA2、RWA3或RWA4。
当在描述具有基本上集中在特定组织的木质素沉积和/或木聚糖沉积的植物的背景下使用时，术语“基本上集中”表示，与通常具有高木质素和/或木聚糖含量的其它细胞类型(诸如维管束间纤维或韧皮纤维)相比，在特定目标细胞类型中以明显更高的量产生的木质素沉积和/或木聚糖沉积。在某些实施方案中，当在特定目标细胞类型中的木质素沉积和/或木聚糖沉积的量是在通常具有高木质素和/或木聚糖含量的其它细胞类型中的木质素沉积和/或木聚糖沉积的量的至少2倍、3倍、4倍、5倍、6倍、7倍、8倍、9倍、10倍或更多时，木质素沉积和/或木聚糖沉积是基本上集中在特定目标细胞类型。在某些实施方案中，当在特定目标细胞类型中的木质素沉积和/或木聚糖沉积的量是在维管束间纤维或韧皮纤维中的木质素沉积和/或木聚糖沉积的量的至少2倍、3倍、4倍、5倍、6倍、7倍、8倍、9倍、10倍或更多时，木质素沉积和/或木聚糖沉积基本上集中在特定目标细胞类型。在某些实施方案中，当在除了特定目标细胞类型以外的细胞类型中不存在可检测的木质素沉积和/或木聚糖沉积时，木质素沉积和/或木聚糖沉积基本上集中在特定目标细胞类型。在某些实施方案中，木聚糖O-乙酰化类似地基本上集中在特定细胞类型，而木聚糖含量一般不一定以不同于天然(即，野生型)情形的方式基本上集中。使用本领域已知的任意方法，可以评估木质素沉积和/或木聚糖沉积，所述方法包括、但不限于使用乙酰基溴试剂的分光光度法、组织化学染色(例如，用间苯三酚)和免疫组织化学(例如，用LM10单克隆抗体)。使用免疫组织化学(例如，用LM23单克隆抗体)，用乙酰酯的生化测定，或通过确定水解酶的作用，可以评估木聚糖O-乙酰化。
本文中使用的术语“调节生物合成途径的组分的产生的转录因子”或“主要转录因子”表示，调节生物合成途径中的一个或多个基因的表达的转录因子。
本文中使用的术语“调节次生细胞壁的产生的转录因子”表示，通过调控转录而调节在木质素生物合成和/或多糖(纤维素和半纤维素)生物合成中涉及的一个或多个基因的表达的多肽和所述多肽的变体、突变体和同系物。在某些实施方案中，编码这样的转录因子的核酸：(1)具有这样的核酸序列，其与SEQ ID NO:13、15、17、19、21、23、25、27、29、31或33中的任一个的核酸序列具有大于约50％核苷酸序列同一性、55％、60％、65％、70％、75％、80％、85％、90％、优选地91％、92％、93％、94％、95％、96％、97％、98％或99％或更高的核苷酸序列同一性，优选地在至少约10个、15个、20个、25个、50个、100个、200个、500个或更多个核苷酸的区域内或在整个多核苷酸的长度内；(2)编码这样的多肽，所述多肽的氨基酸序列与由SEQ ID NO:13、15、17、19、21、23、25、27、29、31或33中的任一个的核酸序列编码的多肽、或与SEQ ID NO:14、16、18、20、22、24、26、28、30、32或34中的任一个的氨基酸序列、或与图13所示的任一个氨基酸序列具有大于约50％氨基酸序列同一性、55％、60％、65％、70％、75％、80％、85％、90％、优选地91％、92％、93％、94％、95％、96％、97％、98％或99％或更大的氨基酸序列同一性，优选地在至少约25个、50个、100个、200个或更多个氨基酸的区域内或在整个多肽的长度内。在某些实施方案中，调节次生细胞壁产生的转录因子多肽:(1)具有这样的氨基酸序列，其与SEQ ID NO:14、16、18、20、22、24、26、28、30、32或34中的任一个的氨基酸序列、或与图13所示的任一个氨基酸序列具有大于约50％氨基酸序列同一性、55％、60％、65％、70％、75％、80％、85％、90％、优选地91％、92％、93％、94％、95％、96％、97％、98％或99％或更大的氨基酸序列同一性，优选地在至少约25个、50个、100个、200个或更多个氨基酸的区域内或在整个多肽的长度内。
在某些实施方案中，所述转录因子是NAC次生壁增厚促进因子1(NST1)(ANAC043；登录号NM_130243或NP_182200)、NST2(ANAC066；登录号NM_116056或NP_191750)、NST3(SND1/ANAC012；登录号NM_103011或NP_174554)、次生壁相关的NAC结构域蛋白2(SND2)(ANAC073；登录号NM_118992或NP_194579)、SND3(ANAC010；登录号NM_102615或NP_564309)、MYB结构域蛋白103(MYB103)(登录号NM_105065或NP_176575)、MBY85(登录号NM_118394或NP_567664)、MYB46(登录号NM_121290或NP_196791)、MYB83(登录号NM_111685或NP_187463)、MYB58(登录号NM_101514或NP_173098)或MYB63(登录号NM_106569或NP_178039)。
当在调节目标生物合成途径的组分的转录因子的下游靶标的背景下使用时，术语“下游靶标”表示这样的基因或蛋白：其表达直接地或间接地由所述转录因子调节。在某些实施方案中，所述下游靶标是被所述转录因子直接地或间接地增量调节的基因或蛋白。在某些实施方案中，所述下游靶标是被所述转录因子直接地或间接地减量调节的基因或蛋白。
在次生壁产生的背景下，下游靶标可以是，例如，IRX1、IRX3、IRX5、IRX8、IRX9、IRX14、IRX14-L、IRX7或IRX10。关于下游靶标的登录号和序列的例子，参见例如图7-12。在本领域中还描述了下游靶基因；参见，例如，Oikawa等人,2010,PLoS ONE 5(11):e15481。如本领域理解的和在下文中进一步解释的，一些下游靶标(例如，IRX9-Like和RWA2)本身不可在次生壁组织中表达，但是可以与次生壁特异性的启动子或导管特异性的启动子(所述启动子受调节次生壁产生的转录因子调节)连接，并且然后可以起将木聚糖或木聚糖乙酰化基本上集中在次生壁的作用。
本文中使用的术语调节“蜡和/或角质”组分(例如，蜡酯、烷烃、脂肪醇和脂肪酯)产生的转录因子表示，通过调控转录而调节在蜡和/或角质生物合成中涉及的一个或多个基因的表达的多肽和所述多肽的变体、突变体和同系物。在某些实施方案中，编码这样的转录因子的核酸：编码具有特定氨基酸序列的多肽，所述特定氨基酸序列与由SEQ ID NO:80-93中的任一个的核酸序列编码的多肽、或与SEQ ID NO:80-93中的任一个的氨基酸序列具有大于约50％氨基酸序列同一性、55％、60％、65％、70％、75％、80％、85％、90％、优选地91％、92％、93％、94％、95％、96％、97％、98％或99％或更大的氨基酸序列同一性，优选地在至少约25个、50个、100个、200个或更多个氨基酸的区域内或在整个多肽的长度内。
当在调节蜡/角质产生的转录因子的背景下使用时，“下游靶标”表示在蜡/角质产生中涉及的非编码RNA、基因或蛋白，其表达直接地或间接地由所述转录因子调节。在某些实施方案中，所述下游靶标是直接地或间接地由所述转录因子增量调节的非编码RNA、基因或蛋白。在某些实施方案中，所述下游靶标是直接地或间接地由所述转录因子减量调节的非编码RNA、基因或蛋白。这样的基因的例子包括下述的(所述基因的同义词在括号中列出)：CER1，醛脱羰酶；CER2(VC2)，BAHD-型酰基–转移酶；CER3(WAX2)，甾醇去饱和酶；CER4(FAR3)，脂肪酰基辅酶A还原酶；CER5(WBC12)，ABC转运蛋白；CER6(CUT1)，极长链脂肪酸缩合酶；CER10(ECR)，烯酰辅酶A还原酶；WSD1，蜡酯合酶；MAH1，中链烷烃水解酶；WBC11(ABCG11，DSO，COF1)，ABC转运蛋白；KCS1，极长链脂肪酸缩合酶；KCS2(DAISY)，极长链脂肪酸缩合酶；FATB，酰基载体；LACS1，长链酰基辅酶A合酶；LACS2，长链酰基辅酶A合酶；CYP86A4，细胞色素P450依赖性的脂肪酸羟化酶；CYP86A7，细胞色素P450依赖性的脂肪酸羟化酶；LCR(CYP86A5)，细胞色素P450依赖性的脂肪酸羟化酶；KCS10(FDH)，极长链脂肪酸缩合酶；和CER60(KCS5)，极长链脂肪酸缩合酶。在示例性的蜡/角质基因列表中提供了登录号的例子。
术语“降低的活性水平”、“减少的活性”和“降低的活性”可互换地表示，与在野生型(即，天然存在的)植物中的活性量相比，在经工程改造的植物中的蛋白(例如，目标细胞壁生物合成酶或目标木聚糖生物合成酶基因或蛋白)的活性量的下降。在某些实施方案中，减少的活性源自降低的表达水平。降低的活性水平或降低的表达水平可以是蛋白(例如，细胞壁生物合成酶基因或蛋白或木聚糖生物合成酶基因或蛋白)的活性或表达的量的下降了至少10％、20％、30％、40％、50％、60％、70％、80％或90％或更大。在某些实施方案中，所述降低的活性水平或降低的表达水平是酶(例如，目标细胞壁生物合成酶基因或蛋白或目标木聚糖生物合成酶基因或蛋白)在经工程改造的植物的所有组织中的活性或表达的量的下降。在某些实施方案中，所述蛋白或基因(例如，目标细胞壁生物合成酶基因或蛋白或目标木聚糖生物合成酶基因或蛋白)的活性或表达的量的下降集中在经工程改造的植物的一个或多个组织。在某些实施方案中，所述生物合成酶的量没有下降，但是氨基酸序列经过修饰，使得酶活性直接地或间接地下降(例如，通过抑制性蛋白的表达)。通过测量由目标基因编码的RNA的水平的下降和/或蛋白表达水平或目标蛋白活性的下降，可以评估基因或蛋白的表达量的下降。
术语“多核苷酸”和“核酸”互换使用，并表示从5'末端至3'末端读出的脱氧核糖核苷酸或核糖核苷酸碱基的单链或双链聚合物。本发明的核酸通常含有磷酸二酯键，尽管在某些情况下，可使用可能具有替代主链的核酸类似物，其包括例如，氨基磷酸酯、硫代磷酸酯、二硫代磷酸酯或O-甲基亚磷酰胺连接(参见Eckstein,Oligonucleotides andAnalogues:A Practical Approach,Oxford University Press)；带正电主链、非离子主链和非核糖主链。因此，核酸或多核苷酸还可能包含经修饰的核苷酸，其允许被聚合酶正确阅读。“多核苷酸序列”或“核酸序列”包括作为单独单链或在双链体中的核酸的有义链和反义链。本领域技术人员会理解，对单链的描述还限定了互补链的序列；因此本文中所描述的序列也提供它的互补序列。除非另外说明，具体的核酸序列也隐含地涵盖了其变体(例如简并密码子取代物)和互补序列，明确说明的序列。核酸可以是DNA，包括基因组和cDNA、RNA或杂交体，其中核酸可能包含脱氧核糖核苷酸和核糖核苷酸的组合，以及碱基的组合，其包括尿嘧啶、腺嘌呤、胸腺嘧啶、胞嘧啶、鸟嘌呤、肌苷、黄嘌呤次黄嘌呤、异胞嘧啶、异鸟嘌呤等
在2个核酸或多肽的背景下使用的术语“基本上相同的”表示，与参照序列具有至少50％序列同一性的序列。同一性百分比可以是从50％至100％的任意整数。一些实施方案至少包括：使用本文描述的程序，优选BLAST(使用如下所述的标准参数)，与参照序列相比，50％、55％、60％、65％、70％、75％、80％、85％、90％、91％、92％、93％、94％、95％、96％、97％、98％或99％。例如，编码木质素生物合成酶的多核苷酸可以具有这样的序列，其与SEQID NO:1、SEQ ID NO:3、SEQ ID NO:5、SEQ ID NO:7、SEQ ID NO:9或SEQ ID NO:11的序列具有至少50％、55％、60％、65％、70％、75％、80％、85％、90％、91％、92％、93％、94％、95％、96％、97％、98％或99％同一性。
如果两个序列中的核苷酸序列或氨基酸残基序列在如下所述进行最大对应性比对时是相同的，则这两个核酸序列或多肽序列被称为“相同的”。在2个或更多个核酸或多肽序列的背景下，术语“相同的”或“同一性”百分比表示，当在对比窗中对比和比对最大对应性时，使用下述序列对比算法之一或通过手工比对和目检测得，2个或多个序列或子序列是相同的，或具有指定百分比的相同的氨基酸残基或核苷酸。当关于蛋白或肽的序列同一性百分比使用时，将认识到：不相同的残基位置常常相差保守的氨基酸置换，其中氨基酸残基被其它具有相似化学性质(例如电荷或疏水性)的氨基酸残基替代，因此不会改变分子的功能性质。当序列差别在于保守置换时，可上调序列同一性百分比，以针对置换的保守性质进行校正。进行这样的调节的方法是本领域技术人员众所周知的。典型地，这包括：将保守置换计分为部分错配而非完全错配，由此增加序列同一性百分比。因此，例如，当相同的氨基酸被给予1分且非保守置换被给予0分时，保守置换被给予0至1之间的得分。根据例如Meyers和Miller,Computer Applic.Biol.Sci.4:11-17(1988)的算法，计算保守置换的得分，例如，在程序PC/GENE(Intelligenetics,Mountain View,California,USA)中实现。
对于序列对比，通常一个序列作为参照序列，测试序列与其进行对比。当使用序列对比算法时，将测试序列和参照序列输入到计算机中，指定子序列坐标，如有必要，指定序列算法程序参数。可以使用默认程序参数，或者可以指定替代参数。序列对比算法然后基于程序参数，计算测试序列相对于参照序列的序列同一性百分比。
本文中使用的“对比窗”包括对选自20至600、经常约50至约200、更经常约100至约150的连续位置数目中的任一个数目的区段的提及，在所述区段中，可以在将序列与具有相同连续位置数目的参照序列最佳比对后，将这两条序列进行对比。为了对比而比对序列的方法是本领域众所周知。用于对比的最佳序列比对可以通过如下方法进行，例如，Smith和Waterman,Adv.Appl.Math.2:482(1981)的局部同源性算法，Needleman和Wunsch,J.Mol.Biol.48:443(1970)的同源性比对算法，通过Pearson和Lipman,Proc.Nat'l.Acad.Sci.USA 85:2444(1988)的相似性检索方法，通过这些算法的计算机化实现(GAP、BESTFIT、FASTA和TFASTA，在Wisconsin Genetics Software Package中，GeneticsComputer Group,575Science Dr.,Madison,WI)，或通过手工比对和目检。
适合用于确定序列同一性百分比和序列相似性的算法是BLAST和BLAST 2.0算法，它们分别描述在Altschul等人(1990)J.Mol.Biol.215:403-410和Altschul等人(1977)Nucleic Acids Res.25:3389-3402中。用于执行BLAST分析的软件在国家生物技术信息中心(http://www.ncbi.nlm.nih.gov/)是公众可获得的。这个算法包括，首先通过在查询序列中鉴定长度W的短字来鉴定高分序列对(HSP)，当与数据库序列中相同长度的字比对时，高分序列对匹配或满足某些正评估的阈值分T。T被称为邻近字分数阈值(Altschul等人,出处同上)。这些最初的邻近字采样充当了开始搜索以发现含有它们的更长的HSP的种子。然后沿着每个序列的两个方向延伸所述字采样，直到累积的比对计分提高。对于核苷酸序列，使用参数M(匹配的残基对的奖励分；总是>0)和N(错配残基的罚分；总是<0)来计算累积的计分。对于氨基酸序列，使用计分矩阵来计算累积的分数。在下述情况时停止在每个方向上字采样的延伸：累积的比对分数从其达到的最大值下降了数值X；由于一个或多个负分数残基比对的积累，累积的计分达到零或低于零；或到达任何一个序列的结尾。BLAST算法的参数W、T和X决定了比对的敏感性和速度。BLASTN程序(对于核苷酸序列)默认使用的是字长度(W)28，期望值(E)10，M＝1，N＝-2，并对比两个链。对于氨基酸序列，BLASTP程序默认使用的是，字长度(W)3，期望值(E)10，和BLOSUM62计分矩阵(参见Henikoff和Henikoff,Proc.Natl.Acad.Sci.USA 89:10915(1989))。
BLAST算法还进行两个序列之间的相似性统计分析(参见，例如，Karlin和Altschul,Proc.Nat'l.Acad.Sci.USA 90:5873-5787(1993))。BLAST算法提供的一种相似性测量法是最小总和概率(P(N))，其提供了在两个核苷酸或氨基酸序列之间可能偶然出现匹配的概率指示。例如，如果在与参照核酸对比测试核酸时最小总和概率小于约0.01、更优选地小于约10-5和最优选地小于约10-20，那么认为核酸与参照序列相似。
与参照序列基本上相同的核酸或蛋白序列包括“经保守修饰的变体”。对于特定的核酸序列，经保守修饰的变体指编码相同或基本相同氨基酸序列的那些核酸，或当核酸不编码氨基酸序列时，指基本相同的序列。由于遗传密码的简并性，大量功能相同的核酸编码任意给定的蛋白质。例如，密码子GCA、GCC、GCG和GCU都编码氨基酸丙氨酸。因此，在丙氨酸由密码子确定的每一个位点，密码子可以变化成任何所述的相应的密码子而不改变编码的多肽。这样的核酸变异是“沉默变异”，它们是一类保守修饰的变异。本文中编码多肽的每种核酸序列也描述核酸的每种可能的沉默变异。技术人员将认识到核酸中的每种密码子(除了AUG，该密码子通常是甲硫氨酸的唯一密码子)都可被修饰，而能生成功能相同的分子。因此，编码多肽的核酸的每种沉默变异都包含在各所述序列中。
关于氨基酸序列，技术人员将认识到当改变导致氨基酸被化学相似的氨基酸置换时，在核酸、肽、多肽或蛋白质序列中改变被编码序列中的单个氨基酸或一小部分氨基酸的各种置换是“保守修饰的变异”。提供功能相似氨基酸的保守置换表是本领域公知的。
以下六组每组都包含相互之间为保守置换的氨基酸：
1)丙氨酸(A)、丝氨酸(S)、苏氨酸(T)；
2)天冬氨酸(D)、谷氨酸(E)；
3)天冬酰胺(N)、谷氨酰胺(Q)；
4)精氨酸(R)、赖氨酸(K)；
5)异亮氨酸(I)、亮氨酸(L)、蛋氨酸(M)、缬氨酸(V)；和
6)苯丙氨酸(F)、酪氨酸(Y)、色氨酸(W)。
(参见，例如，Creighton,Proteins(1984))。
核苷酸序列基本上相同的另一种表示是，在严谨条件下，2种分子彼此杂交或与第三种核酸杂交。严谨条件是序列依赖性的，并且在不同的情况下是不同的。通常，选择的严谨条件比特定序列在确定的离子强度和pH下的热熔点(Tm)低约5℃。Tm是这样的温度(在确定的离子强度和pH下)：在该温度，50％的靶序列与完美匹配的探针杂交。通常，严谨条件是这样的条件：其中盐浓度为约0.02摩尔，在pH 7，且温度为至少约60℃。例如，用于杂交(诸如在印迹技术中的RNA-DNA杂交)的严谨条件是包括在0.2X SSC中在55℃洗涤至少1次20分钟的条件或等效条件。
本文中使用的术语“启动子”表示，能够驱动细胞中的DNA序列的转录的多核苷酸序列。因而，在本发明的多核苷酸构建体中使用的启动子包括，在调节或调控基因转录的时机和/或速率中涉及的顺式-和反式-作用转录控制元件和调节序列。例如，启动子可以是顺式作用转录控制元件，包括在转录调节中涉及的增强子、启动子、转录终止子、复制起点、染色体整合序列、5'和3'非翻译区或内含子序列。这些顺式作用序列通常与蛋白或其它生物分子相互作用以影响(开启/关闭、调节、调控等)基因转录。启动子位于转录基因的5'侧，且如本文中使用的，包括在翻译起始密码子的5'侧的序列(即，包括mRNA的5'非翻译区，通常包含100-200bp)。最常见地，核心启动子序列位于翻译起始位点的1-2kb内，更经常在1kbp内，且经常在翻译起始位点的500bp内。按照惯例，启动子序列经常被提供为在它控制的基因的编码链上的序列。在该应用的背景下，通常用启动子天然地调节其表达的基因的名称来表示所述启动子。通过基因的名称来表示在本发明的表达构建体中使用的启动子。通过名称对启动子的提及包括野生型天然启动子以及所述启动子的保留诱导表达的能力的变体。通过名称对启动子的提及不限于特定植物物种，而是也包括得自其它植物种内的对应基因的启动子。
“组成型启动子”在本发明范围内表示能够在几乎所有细胞类型中启动转录的启动子，而“细胞类型特异性的启动子”或“组织特异性的启动子”仅在一种或几种特定细胞类型中或在形成组织的细胞群中启动转录。在某些实施方案中，如果启动子在特定细胞类型或组织中启动的转录水平是所述启动子在非导管组织中启动的转录水平的至少2倍、3倍、4倍、5倍、6倍、7倍、8倍、9倍、10倍、50倍、100倍、500倍、1000倍或更高，那么该启动子是组织特异性的。在某些实施方案中，所述启动子是导管特异性的。本文中使用的“导管特异性的”启动子表示这样的启动子：与植物的其它非导管细胞相比，其在导管中启动明显更高的转录水平。本文中使用的术语“导管”表示木质导管，即植物中的维管组织的传导组件，其在水、营养物和信号传递分子在植物中的运输中起作用。在某些实施方案中，如果启动子在导管组织中启动的转录水平是所述启动子在非导管组织中启动的转录水平的至少2倍、3倍、4倍、5倍、6倍、7倍、8倍、9倍、10倍、50倍、100倍、500倍、1000倍或更高，那么该启动子是导管特异性的。导管特异性的启动子的非限制性例子包括，编码维管相关的NAC-结构域蛋白1(VND1)、VND2、VND3、VND4、VND5、VND6、VND7的任意基因的天然启动子。参见，例如，Kubo等人,Genes Dev.19:1855-1860(2005)，它通过引用并入本文。导管特异性的启动子的另一个例子包括REF4和RFR1的天然启动子(参见，例如，Bonawitz等人,“The REF4 and RFR1subunits of the eukaryotic transcriptional coregulatory complex Mediator arerequired for phenylpropanoid homeostasis in Arabidopsis.”doi:10.1074/jbc.M111.312298(2012))。
在人工正反馈回路的背景下，在目标生物合成途径的基因的下游的“诱导型”启动子表示这样的启动子：其中所述基因的表达被增强，即，其表达可以直接地或间接地被在人工正反馈回路中采用的转录因子活化(开启和/或增加)。因而，当提及在人工反馈回路构建体中采用的启动子时，应当理解，所述启动子被转录因子“诱导”，不论是否明确地阐明所述启动子是诱导型启动子。
在下述情况下，多核苷酸相对于生物体或第二种多核苷酸序列而言是“异源”：所述多核苷酸源自外来物种，或者，如果源自相同物种，其从它的原始形式经过修饰。例如，当将编码多肽序列的多核苷酸说成与异源启动子可操作地连接时，是指，编码所述多肽的多核苷酸编码序列源自一个物种，而所述启动子序列源自另一个不同的物种；或者，如果二者源自相同的物种，所述编码序列天然地不与所述启动子结合(例如，是遗传工程改造的编码序列，例如，从相同物种的不同基因改成，或得自不同生态型或变种的等位基因)。
术语“可操作地连接”表示，2个2个或更多个多核苷酸(例如，DNA)区段之间的功能关联。通常，它表示转录调节序列与被转录的序列的功能关联。例如，在下述情况下，启动子或增强子序列与DNA或RNA序列可操作地连接：其在适当的宿主细胞或其它表达系统中刺激或调控所述DNA或RNA序列的转录。通常，与被转录的序列可操作地连接的启动子转录调节序列与所述被转录的序列是物理上连续的，即，它们顺式地起作用。但是，有些转录调节序列(诸如增强子)不需要与它们增强其转录的编码序列在物理上连续或位于紧邻处。
术语“表达盒”或“DNA构建体”或“表达构建体”表示这样的核酸构建体：当被引入宿主细胞中时，其分别导致RNA或多肽的转录和/或翻译。该定义明确地包括未翻译或不可翻译的反义或有义构建体。在表达转基因和抑制内源基因(例如，通过反义、RNAi或有义抑制)的情况下，技术人员会认识到，插入的多核苷酸序列不需要是相同的，但是可以仅仅与它的来源基因的序列基本上相同。如本文中解释的，对特定核酸序列的提及明确地涵盖这些基本上相同的变体。表达盒的一个例子是，包含与异源启动子可操作地连接的转录因子的多核苷酸构建体，所述异源启动子是得自由所述转录因子调节的基因的启动子。
本文中使用的术语“植物”可以表示完整植物或植物的部分，例如，种子，且包括多种倍性水平的植物，包括非整倍体、多倍体、二倍体和单倍体。本文中使用的术语“植物部分”表示枝条营养器官和/或结构(例如，叶、茎和块茎)、枝、根、花和花器(例如，苞片、萼片、花瓣、雄蕊、心皮、花药)、胚珠(包括卵细胞和中央细胞)、种子(包括合子、胚、胚乳和种皮)、果实(例如，成熟的子房)、幼苗和植物组织(例如，维管组织、基本组织等)、以及单个植物细胞、植物细胞群(例如，培养的植物细胞)、原生质体、植物提取物和种子。在本发明的方法中可以使用的植物的种类通常宽泛至顺从转化技术的高等和低等植物种类，包括被子植物(单子叶和双子叶植物)、裸子植物、蕨类植物、苔藓植物和多细胞的藻类。
本文中使用的术语“生物质”表示这样的植物材料：其被加工以提供产品，例如，生物燃料诸如乙醇、或家畜饲料、或用于纸和纸浆工业产品的纤维素。这样的植物材料可以包括完整植物或植物的部分，例如，茎、叶、枝、芽、根、块茎等。
术语“增加的次生细胞壁沉积”表示，与野生型(即，天然存在的)植物相比，在本发明的经工程改造的植物中产生增加的量的次生细胞壁，例如，增加的密度或厚度和/或增加的细胞直径和细胞壁厚度之比。“次生细胞壁”主要由纤维素、半纤维素和木质素组成，且沉积在植物的一些(但并非所有)组织(诸如木质组织)中。在下述情况下，将次生细胞壁沉积说成与野生型植物相比在经工程改造的植物中增加：与在野生型植物中的次生细胞壁的一种或多种组分的量相比，在经工程改造的植物中的次生细胞壁的一种或多种组分(例如，纤维素、半纤维素或木质素)的量，或细胞直径和细胞壁厚度之比，增加了至少10％、至少20、30％、40％、50％、60％、70％、80％、90％或更多。使用本领域已知的任意方法，可以评估存在的次生细胞壁的组分的量，所述方法包括、但不限于显微术(例如，电子显微术、RAMAN-显微术)、组织化学染色(例如，间苯三酚)和酶或化学反应(例如，多糖水解或TFA水解)。
术语“糖化反应”表示，将生物质(经常是纤维质或木质纤维素生物质)转化成单体糖(诸如葡萄糖和木糖)的过程。
术语“可溶性糖”表示，从生物质的糖化产生的单体糖、二聚体糖或三聚体糖。
当表示从本发明的经工程改造的植物得到的糖或可溶性糖的量时，术语“增加的量”表示，与得自野生型(即，天然存在的)植物的对应生物质相比，从单位量的起始原料的生物质糖化得到的糖的量或产量的增加。在本发明范围内，“得自野生型植物的对应生物质”表示这样的植物材料：其得自与具有降低的木质素生物合成酶表达水平和/或木聚糖生物合成酶的植物的生物质相同的植物部分。如本领域理解的,增加的量或增加的产量是基于相同量的对应植物材料的对比。
本文中使用的术语“转化反应”表示，将生物质转化成生物能形式的反应。转化反应的例子包括、但不限于：燃烧(烧)、气化、热解和多糖水解(酶法或化学法)。
当表示从本发明的经工程改造的植物得到的生物能生产量时，术语“增加的生产量”表示，与从得自野生型(即，天然存在的)植物的对应生物质产生的生物能的量相比，当对得自经工程改造的植物的生物质进行转化反应(例如，燃烧、气化、热解或多糖水解)时产生的生物能的量增加。
II.简介
在一个方面，本发明涉及下述发现：可以在植物中建立人工正反馈回路(APFL)，以调节期望的生物合成途径中的基因表达，例如，以调控一个或多个期望的组织中的基因表达。因此，本发明提供了植物中的APFL，其中所述APFL包含编码转录因子的基因，所述转录因子控制目标生物合成途径的表达，所述基因与所述生物合成途径中的诱导型下游基因的启动子可操作地连接，其中所述下游基因的表达由所述转录因子控制。可以由这样的系统调节的生物合成途径的例子包括：次生细胞壁沉积、蜡/角质生物合成、脂质生物合成、生物碱生物合成和萜类化合物生物合成。因而，根据本发明的APFL的一个例子涉及，增加特定组织中的细胞壁沉积，其中将编码本文所述的控制次生细胞壁生物合成的转录因子的核酸与在次生壁生物合成中涉及的下游诱导型基因的启动子可操作地连接，其中所述下游基因的表达由所述转录因子诱导。本发明的APFL的第二个例子包含：编码本文所述的控制蜡和/或角质的表达生物合成的转录因子的核酸，所述核酸与在蜡和/或角质生物合成中涉及的下游诱导型基因的启动子可操作地连接，其中所述下游基因的表达由所述转录因子诱导。本发明的APFL的另一个例子包含：编码本文所述的调节脂质生物合成和例如在种子和其它组织中的积累的转录因子的核酸，所述核酸与在脂质生物合成中涉及的下游诱导型基因的启动子可操作地连接，其中所述下游基因的表达由所述转录因子诱导。
在不同的实施方案中，本发明提供了包含本发明的AFPL的核酸、表达构建体和植物以及使用这样的组合物的方法。
在一个方面，本发明部分地基于下述发现：将木质素沉积集中在植物导管中，同时降低植物别处的木质素和/或木聚糖含量，会克服通常与具有降低的木质素或木聚糖含量的植物有关的问题，特别是导管塌缩和植物发育迟缓。尽管就诸如给导管(其在植物中供给水和营养物)提供结构支持等目的而言细胞壁组分(诸如木质素和木聚糖)对植物是有益的，但是这些细胞壁组分(例如，木质素和木聚糖)也是细胞壁不顺从酶促降解和多糖可提取性的主要原因。因此，木质素和木聚糖在导管中的特异性集中代表了这样的方法：通过该方法，可以使得植物的细胞壁更易于酶促降解和多糖可提取性，从而改善从植物的糖化和例如生物燃料生产；并且，也给纸和纸浆工业提供改进的底物。因此，在一个方面，本发明提供了工程改造植物的方法，所述植物具有基本上集中在植物木质部组织导管的木质素和/或木聚糖沉积和/或木聚糖O-乙酰化。如下完成导管特异性的木质素和/或木聚糖沉积和/或木聚糖O-乙酰化：减少木质素和/或木聚糖生物合成酶和/或木聚糖O-乙酰化酶，并在导管特异性的启动子控制下表达基本上相同的酶(例如，在所述植物中减少的酶的直系同源物或旁系同源物，或具有相同生化功能的酶)，所述启动子不是木质素和/或木聚糖生物合成酶和/或木聚糖O-乙酰化酶的天然启动子。本发明的植物或包含本发明的植物的生物质适合用在糖化反应中，以得到与从野生型植物可以得到的量相比增加的量的可溶性糖，或者用在造纸工业中。
本发明也部分地基于下述发现：特异性地增加在木质组织中的细胞壁沉积会产生这样的植物，所述植物具有被细胞壁聚合物填充的细胞。增加的细胞壁沉积是有益的，因为它会增加植物的生物质密度，后者又可以增加可以从所述植物得到的生物能生产量。因此，在另一个方面，本发明提供了使用AFPL工程改造植物的方法，所述植物具有增加的细胞壁沉积。在作为转录因子下游靶标的诱导型基因的启动子的控制下，在植物中表达调节次生细胞壁产生的转录因子。所述转录因子的表达会增加由所述下游启动子驱动的表达，因为所述启动子与编码所述转录因子的基因可操作地连接，这又会增加所述转录因子的表达，从而产生正反馈回路，该回路会增强次生细胞壁途径的下游基因的表达，并由此增加次生细胞壁沉积。所述转录因子和启动子可以得自与宿主植物不同的植物种，或者所述转录因子或启动子可以得自不同的植物种。类似地，所述转录因子和启动子不需要得自相同的植物种。本发明的植物或包含本发明的植物的生物质适合用在生物质转化反应中，以与野生型植物的生物能生产量相比增加生物能生产量。
本发明的方法可以进一步彼此联合使用。因而，在某些实施方案中，本发明提供了制备植物的方法，所述植物具有增加的基本上集中在植物木质部组织导管的木质素沉积，且具有增加的次生细胞壁沉积。在某些实施方案中，本发明提供了制备植物的方法，所述植物具有增加的基本上集中在植物木质部组织导管的木聚糖沉积，且具有增加的次生细胞壁沉积。在某些实施方案中，本发明提供了制备植物的方法，所述植物具有增加的基本上集中在植物木质部组织导管的木聚糖O-乙酰化沉积，且具有增加的次生细胞壁沉积。在某些实施方案中，本发明提供了制备植物的方法，所述植物具有增加的基本上集中在植物木质部组织导管的木质素沉积，且具有增加的基本上集中在植物木质部组织导管的木聚糖沉积。在某些实施方案中，本发明提供了制备植物的方法，所述植物具有基本上集中在植物木质部组织导管的木质素沉积，且具有增加的基本上集中在植物木质部组织导管的木聚糖O-乙酰化沉积。
在另一个方面，本发明提供了一种增加期望的组织中的蜡/角质产生的方法。在作为转录因子下游靶标的诱导型基因的启动子的控制下，在植物中表达调节蜡/角质层产生的转录因子。所述转录因子的表达会增加由所述下游启动子驱动的表达，因为所述启动子与编码所述转录因子的基因可操作地连接，这又会增加所述转录因子的表达，从而产生增加蜡/角质产生的正反馈回路。所述转录因子和启动子、或者所述转录因子或启动子可以得自与在其中建立人工正反馈回路的宿主植物细胞不同的物种。在某些实施方案中，所述转录因子和启动子得自不同的物种。根据本发明的该方面制备的植物具有增加的干旱耐受性和降低的水消耗。
III.具有经空间修饰的基因表达的植物
A.木质素或木聚糖生物合成酶的表达的修饰
在一个方面，本发明提供了工程改造植物的方法，所述植物具有基本上集中在植物木质部组织导管的木质素沉积。在某些实施方案中，所述方法包括：
将表达盒引入植物中，其中所述植物被修饰成具有降低的木质素生物合成酶表达水平；且其中所述表达盒包含与异源的导管特异性的启动子可操作地连接的编码木质素生物合成酶的多核苷酸；和
在表达木质素生物合成酶的条件下，培养所述植物。
在另一个方面，本发明提供了工程改造植物的方法，所述植物具有基本上集中在植物木质部组织导管的木聚糖沉积。在某些实施方案中，所述方法包括：
将表达盒引入植物中，其中所述植物被修饰成具有降低的木聚糖生物合成酶表达水平；且其中所述表达盒包含与异源的导管特异性的启动子可操作地连接的编码木聚糖生物合成酶的多核苷酸；和
在表达所述木聚糖生物合成酶的条件下，培养所述植物。
当被引入修饰成具有降低的木质素或木聚糖生物合成酶表达水平的植物中时，在本文中描述的表达盒会产生具有精细调节的木质素或木聚糖沉积的植物，其中木质素仍然在导管组织中表达，从而防止导管塌缩，但是其中木质素或木聚糖在其它组织中不高度表达，从而减少细胞壁不顺从。
本领域技术人员会理解，被表达盒引入植物中的木质素生物合成酶和/或木聚糖生物合成酶不一定与在引入所述表达盒之前在植物中修饰的木质素生物合成酶和/或木聚糖生物合成酶相同。在某些实施方案中，被表达盒引入植物中的木质素生物合成酶和/或木聚糖生物合成酶与在引入所述表达盒之前在植物中修饰的木质素生物合成酶和/或木聚糖生物合成酶基本上相同(例如，至少50％、至少55％、至少60％、至少65％、至少70％、至少75％、至少80％、至少85％、至少90％、至少91％、至少92％、至少93％、至少94％、至少95％、至少96％、至少97％、至少98％或至少99％同一)。在某些实施方案中，被表达盒引入植物中的木质素生物合成酶和/或木聚糖生物合成酶是在引入所述表达盒之前在植物中修饰的木质素生物合成酶和/或木聚糖生物合成酶的同系物(例如，图1-12中的任一个比对所示的同系物，或具有相同生化功能的酶，例如，旁系同源物)。
1.木质素生物合成酶
在某些实施方案中，所述表达盒包含编码木质素生物合成酶的多核苷酸。基于调节木质醇单体产生并因此调节木质素生物合成，可以选择用于本发明中的木质素生物合成酶。在某些实施方案中，所述木质素生物合成酶是苯丙氨酸氨裂合酶(PAL)、肉桂酸4-羟化酶(C4H)、4-香豆酸-CoA连接酶(4CL)、羟基肉桂酰辅酶A:莽草酸羟基肉桂酰基转移酶(HCT)、香豆酰基莽草酸3-羟化酶(C3H)或肉桂酰基辅酶A还原酶1(CCR1)。
已经在拟南芥属中表征了木质素生物合成酶PAL、C4H、4CL、HCT、C3H和CCR1，并且已经证实它们会介导从苯丙氨酸合成木质素单体(木质醇单体)。参见，例如，Bonawitz和Chapple,Annu.Rev.Genet.44:337-63(2010)。因而，在某些实施方案中，所述编码木质素生物合成酶的多核苷酸与SEQ ID NO:1、3、5、7、9或11中的任一个多核苷酸序列基本上相同。在某些实施方案中，所述木质素生物合成酶与SEQ ID NO:2、4、6、8、10或12中的任一个多肽序列基本上相同。另外，在木质素生物合成中涉及的许多酶在物种之间是保守的。因而，在某些实施方案中，所述编码木质素生物合成酶的多核苷酸包含SEQ ID NO:1、3、5、7、9或11中的任一个多核苷酸序列的同系物。在某些实施方案中，所述木质素生物合成酶包含SEQID NO:2、4、6、8、10或12中的任一个多肽序列或图1-6中的任一图所示的任一个多肽序列的同系物。
在某些实施方案中，所述编码木质素生物合成酶的多核苷酸包含与SEQ ID NO:1、3、5、7、9或11中的任一个基本上相同(例如，至少50％、至少55％、至少60％、至少65％、至少70％、至少75％、至少80％、至少85％、至少90％、至少91％、至少92％、至少93％、至少94％、至少95％、至少96％、至少97％、至少98％或至少99％同一)的多核苷酸序列。在某些实施方案中，所述编码木质素生物合成酶的多核苷酸包含编码特定多肽序列的多核苷酸序列，所述多肽序列与SEQ ID NO:2、4、6、8、10或12中的任一个或图1-6中的任一图所示的任一个多肽序列基本上相同(例如，至少50％、至少55％、至少60％、至少65％、至少70％、至少75％、至少80％、至少85％、至少90％、至少91％、至少92％、至少93％、至少94％、至少95％、至少96％、至少97％、至少98％或至少99％同一)。在某些实施方案中，所述木质素生物合成酶包含与SEQ ID NO:2、4、6、8、10或12中的任一个或图1-6中的任一图所示的任一个多肽序列基本上相同(例如，至少50％、至少55％、至少60％、至少65％、至少70％、至少75％、至少80％、至少85％、至少90％、至少91％、至少92％、至少93％、至少94％、至少95％、至少96％、至少97％、至少98％或至少99％同一)的氨基酸序列。
在本文的序列表中描述了PAL、C4H、4CL、HCT、C3H和CCR1的基因和蛋白序列和/或登录号。在图1-6中显示了木质素生物合成酶的氨基酸序列比对，其中显示了得自多个植物种的这些蛋白中的每一种的氨基酸序列。另外，在本领域中已知并描述了这些蛋白的基因和蛋白序列以及获得所述基因或蛋白的方法。参见，例如，Schilmiller等人,2009,PlantJ.,doi:10.1111/j.1365-313X.2009.03996.x。本领域技术人员会认识到，可以修饰本领域中已知的和/或在本文中描述的这些基因或蛋白序列以制备基本上相同的木质素生物合成酶，例如，通过在一个或多个氨基酸残基处产生保守置换。技术人员还会认识到，已知的序列(例如，本文中提供的比对)会提供关于可以改变哪个氨基酸来制备基本上相同的木质素生物合成酶的指导。例如，使用图1-6所示的任意比对，技术人员会认识到哪个氨基酸残基不是高度保守的，并因而可能进行改变，而不对木质素生物合成酶的功能产生显著影响。
2.木聚糖生物合成酶
本发明的方法也可以采用木聚糖生物合成酶。在木聚糖生物合成中涉及的几种酶是已知的。已经证实在木聚糖生物合成中涉及属于GT43家族(被称作IRX9、IRX9-like、IRX14和IRK14-like)的糖基转移酶(GT)。在这里使用的GT家族的命名法是根据CAZy数据库(www.cazy.org)(Cantarel等人,2009)。还已经证实在木聚糖生物合成中涉及GT47家族的其它GT：IRX10、IRX10-like、IRX7和F8H。另外，已经证实在木聚糖生物合成中涉及GT8中的GT：IRX8(GAUT12)和PARVUS(GATL1)。已知在木聚糖生物合成中涉及所有提及的酶，因为其中基因已经被突变的植物是木聚糖缺陷型(Brown,2009；Wu等人,2010)(Lee等人,2009)(Pena等人,2007；Persson等人,2007；Liepman等人,2010；Scheller和Ulvskov,2010)。在木聚糖生物合成中还涉及术语DUF579家族(也被称作IRX15)的蛋白，尽管它们似乎不是GT(Brown等人,2011)。已经鉴别出负责向木聚糖主链添加葡糖醛酸残基的GT，并且将其称作PGSIP或GUX，但是，这些基因的灭活不会导致木聚糖缺乏(Mortimer等人,2010；Oikawa等人,2010)。已经在文献中将参与向木聚糖主链添加阿拉伯糖残基的GT鉴别为GT61酶家族的成员(Anders等人.2012)。已经鉴别出在多糖(包括木聚糖)的O-乙酰化中涉及的蛋白，并将其命名为RWA蛋白(Manabe等人,2011)，并且已经证实在木糖葡聚糖和甘露聚糖的O-乙酰化中涉及的蛋白是DUF231家族的成员(Gille等人.2011)。最可能的是，木聚糖O-乙酰化需要大DUF231家族的其它成员。
在图7-12中显示了各种IRX蛋白和Parvus蛋白的蛋白序列和登录号。图7-12提供了指定的蛋白的氨基酸序列比对。另外，在本领域中已知并描述了这些蛋白的基因和蛋白序列以及获得所述基因或蛋白的方法。本领域技术人员会认识到，可以修饰本领域中已知的和/或在本文中描述的这些基因或蛋白序列以制备基本上相同的木质素生物合成酶，例如，通过在一个或多个氨基酸残基处产生保守置换。技术人员还会认识到，已知的序列(例如，本文中提供的比对)会提供关于可以改变哪个氨基酸来制备基本上相同的木质素生物合成酶的指导。例如，使用图7-12所示的任意比对，技术人员会认识到哪个氨基酸残基不是高度保守的，并因而可能进行改变，而不对木质素生物合成酶的功能产生显著影响。
除了木聚糖合成基因(例如，在上文中列出的那些)，还可以使用类似的策略通过RWA基因表达来调节多糖O-乙酰化表达谱。RWA蛋白通常在乙酰化(包括在木聚糖O-乙酰化中)中起作用。因而，将RWA的特异性表达与RWA敲除/减量调节相组合，还可以使用本文中描述的技术生产具有非常低的乙酸盐含量、但是仍然具有优良生长性能的植物。在拟南芥属中存在4个RWA基因，且3个(RWA1、RWA3和RWA4)主要在具有次生壁的组织中表达(Manabe等人,2011；)。这些RWA基因中的2个或更多个的减量调节或灭活会导致降低的木聚糖O-乙酰化和受损的维管组织功能(Scheller等人,2010；WO/2010/096488)。因而，可以在植物中减量调节RWA，例如，使用在WO2010/096488中描述的方法和组成，然后将RWA基因重新引入植物中，其中所述RWA基因处于本文所述的启动子/转录因子控制下。作为靶向RWA蛋白的替代方案，可以靶向在木聚糖O-乙酰化中涉及的一种或多种DUF231蛋白。
尽管主要使用拟南芥研究了如上文所述使用的基因和蛋白，在其它植物种中可容易地鉴别出直系同源物。例如，对于许多基因，已经通过互补实验证实，沉默或从其它植物直系同源产生的RNAi具有与拟南芥蛋白相同的功能(Zhou等人,2006；Zhou等人,2007；Lee等人,2009)。
在本文中描述了IRX8、IRX14、IRX14-like、IRX9、IRX9-like、IRX7、IRX10、IRX10-like、IRX15、IRX15-like、F8H和PARVUS的基因和蛋白序列和/或登录号。在图7-12中还显示了木聚糖生物合成酶的氨基酸序列比对，其显示了得自多个植物种的这些蛋白中的每一种的氨基酸序列。另外，如上面所讨论的，在本领域中已知并描述了这些蛋白的基因和蛋白序列以及获得所述基因或蛋白的方法。本领域技术人员会认识到，可以修饰本领域中已知的和/或在本文中描述的这些基因或蛋白序列以制备基本上相同的木质素生物合成酶，例如，通过在一个或多个氨基酸残基处产生保守置换。技术人员还会认识到，已知的序列(例如，本文中提供的比对)会提供关于可以改变哪个氨基酸来制备基本上相同的木聚糖生物合成酶的指导。例如，使用图7-12所示的任意比对，技术人员会认识到哪个氨基酸残基不是高度保守的，并因而可能进行改变，而不对木聚糖生物合成酶的功能产生显著影响。
3.导管特异性的启动子
在某些实施方案中，所述编码木质素生物合成酶或木聚糖生物合成酶的多核苷酸与导管特异性的启动子可操作地连接。所述导管特异性的启动子相对于编码木质素生物合成酶或木聚糖生物合成酶的多核苷酸而言是异源的(即，不是与木质素生物合成酶或木聚糖生物合成酶有关的天然启动子)。在下述情况下，启动子适合用作导管特异性的启动子：所述启动子在植物的导管细胞中强烈表达，但是与其表达被修饰的木质素生物合成酶或木聚糖生物合成酶的天然启动子的表达水平相比，在植物的纤维细胞中以更低的水平表达。
在某些实施方案中，所述启动子与编码维管相关的NAC-结构域1(VND1)、VND2、VND3、VND4、VND5、VND6、VND7或VND-相互作用2(VNI2)的基因的天然启动子基本上相同(例如，至少50％、至少55％、至少60％、至少65％、至少70％、至少75％、至少80％、至少85％、至少90％、至少91％、至少92％、至少93％、至少94％、至少95％、至少96％、至少97％、至少98％或至少99％同一)。在某些实施方案中，所述启动子与编码REF4或RFR1的基因的天然启动子基本上相同(例如，至少50％、至少55％、至少60％、至少65％、至少70％、至少75％、至少80％、至少85％、至少90％、至少91％、至少92％、至少93％、至少94％、至少95％、至少96％、至少97％、至少98％或至少99％同一)。
在某些实施方案中，所述导管特异性的启动子包含SEQ ID NO:36、94或95。在某些实施方案中，所述导管特异性的启动子包含SEQ ID NO:36、94或95的子序列或其变体。在某些实施方案中，所述导管特异性的启动子包含SEQ ID NO:36、94或95的子序列，所述子序列包含所述序列的约50个至约1000个或更多个连续核苷酸。在某些实施方案中，所述导管特异性的启动子包含SEQ ID NO:36、94或95的子序列，所述子序列包含所述序列的50-1000个、50-900个、50-800个、50-700个、50-600个、50-500个、50-400个、50-300个、50-200个、50-100个、75-1000个、75-900个、75-800个、75-700个、75-600个、75-500个、75-400个、75-300个、75-200个、100-1000个、100-900个、100-800个、100-700个、100-600个、100-500个、100-400个、100-300个或100-200个连续核苷酸。
在本领域中还描述了导管特异性的启动子。参见，例如，Yamaguchi等人,2010,Plant Cell；Kubo等人,2009,Genes Dev.；和Yamaguchi等人,2008,Plant J.；它们中的每一篇通过引用整体并入本文。
本领域技术人员会明白，启动子区域可以耐受显著的变异而不减少活性。因而，在某些实施方案中，所述导管特异性的启动子与SEQ ID NO:36、SEQ ID NO:94或SEQ ID NO:95基本上相同(例如，至少50％、至少55％、至少60％、至少65％、至少70％、至少75％、至少80％、至少85％、至少90％、至少91％、至少92％、至少93％、至少94％、至少95％、至少96％、至少97％、至少98％或至少99％同一)。
4.植物的遗传背景
在某些实施方案中，在其中引入包含木质素或木聚糖生物合成酶的表达盒的植物具有这样的遗传背景：其被修饰成具有降低的木质素或木聚糖生物合成酶活性水平。在某些实施方案中，所述植物被修饰成具有在整个植物中降低的木质素或木聚糖生物合成酶活性水平。在某些实施方案中，所述植物被修饰成具有仅在植物的细胞或组织子集中降低的木质素或木聚糖生物合成酶活性水平。根据本领域已知的任意方法，诸如反义、siRNA、微RNA、dsRNA、有义抑制、诱变或使用显性阴性抑制策略，可以修饰植物的遗传背景。在某些实施方案中，降低了蛋白的表达水平。在某些实施方案中，然后使用具有降低的木质素和/或木聚糖生物合成酶活性水平或表达的经修饰的植物来表达表达盒，所述表达盒表达相同的木质素和/或木聚糖生物合成酶，但是在导管特异性的启动子(而不是它的天然启动子)控制下。在某些实施方案中，被表达盒引入植物中的木质素和/或木聚糖生物合成酶与在植物中减少的木质素和/或木聚糖生物合成酶基本上相同的，但是并非完全相同，以便避免被表达盒引入的木质素和/或木聚糖生物合成酶的沉默(例如，可以在被表达盒引入的木质素和/或木聚糖生物合成酶中产生沉默性的核苷酸变化，使得氨基酸序列与在植物中减少的木质素和/或木聚糖生物合成酶相同，但是核苷酸序列不同)。
a)基因沉默技术
在某些实施方案中，通过反义寡核苷酸来抑制木质素或木聚糖生物合成酶的表达。在反义技术中，克隆得自目标基因的核酸区段，并与启动子可操作地连接，使得RNA的反义链会被转录。然后将该表达盒转化进植物中，并产生RNA的反义链。在植物细胞中，已经提出，反义RNA会通过阻止编码目标酶的mRNA的积累来抑制基因表达，参见，例如，Sheehy等人,Proc.Nat.Acad.Sci.USA,85:8805-8809(1988)；Pnueli等人,The Plant Cell 6:175-186(1994)；和Hiatt等人,美国专利号4,801,340。
被转化进植物中的反义核酸序列与要抑制的一个或多个内源基因的至少一部分基本上相同。但是，所述序列不一定完全相同才能抑制表达。因而，仅编码木质素或木聚糖生物合成酶-编码序列的一部分的反义或有义核酸分子可以是用于生产这样的植物：其中木质素或木聚糖生物合成酶的表达受到抑制。对于反义抑制，被引入的序列也不需要相对于初级转录产物或完全加工过的mRNA而言是全长的。通常，可以使用较高的同源性来补偿较短序列的应用。此外，被引入的序列不需要具有相同的内含子或外显子模式，并且非编码区段的同源性可以是同样有效的。在某些实施方案中，可以使用这样的序列：其中至少例如20个、25个、30个、50个、100个、200个或更多个连续核苷酸(至多mRNA全长)与内源木质素或木聚糖生物合成酶mRNA或其补体基本上相同。
还可以使用催化RNA分子或核酶来抑制编码木质素或木聚糖生物合成酶的基因的表达。可能设计这样的核酶：其与基本上任意靶RNA特异性地配对，并在特定位置处切割磷酸二酯主链，由此在功能上灭活靶RNA。在实现该切割时，所述核酶本身没有改变，因而能够重复利用和切割其它分子，使它成为真正酶。核酶序列在反义RNA内的包含会给它们赋予RNA切割活性，由此增加构建体的活性。
已经鉴别出许多类核酶。一类核酶源自许多能够在植物中自我切割和复制的小环状RNA。所述RNA要么单独复制(类病毒RNA)，要么与辅助病毒一起复制(卫星RNA)。例子包括：得自鳄梨日斑病类病毒的RNA，和得自烟草环斑病毒、紫花苜蓿短暂条纹病毒、绒毛烟草斑驳病毒、茄属植物斑驳病毒和地下三叶草斑驳病毒的卫星RNA。在Haseloff等人.Nature,334:585-591(1988)中描述了靶RNA特异性的核酶的设计和应用。
可以用于抑制编码木质素或木聚糖生物合成酶的基因的表达的另一种方法是通过有义抑制(也被称作共抑制)。已经证实，表达盒(其中相对于启动子以有义取向构造核酸)的引入是用于阻断靶基因的转录的有效方式。关于该方法用于调控内源基因表达的例子，参见Napoli等人,The Plant Cell 2:279-289(1990)；Flavell,Proc.Natl.Acad.Sci.,USA 91:3490-3496(1994)；Kooter and Mol,Current Opin.Biol.4:166-171(1993)；和美国专利号5,034,323、5,231,020和5,283,184。
通常，在希望抑制表达的情况下，发生被引入的序列的一些转录。可能发生这样的效应：其中被引入的序列不含有编码序列本身，而是仅含有与在内源序列的初级转录物中存在的序列同源的内含子或非翻译序列。被引入的序列通常与意图抑制的内源序列基本上相同。该最小同一性通常是大于约65％，但是较高的同一性可以造成内源序列表达的更有效抑制。在某些实施方案中，使用具有实质上更大同一性的序列，例如，使用至少约80％、至少约95％或100％同一性。与在下面进一步讨论的反义调节一样，可以设计和试验该效应，以应用于表现出同源性或实质同源性的类似基因家族内的任意其它蛋白。
对于有义抑制，与初级转录产物或完全加工过的mRNA相比，在表达盒中的被引入的序列(需要小于绝对同一性)也不需要是全长的。此外，被引入的序列不需要具有相同的内含子或外显子模式，且非编码区段的同一性是同样有效的。在某些实施方案中，使用具有在上面关于反义调节指出的大小范围(即，30-40个、或至少约20个、50个、100个、200个、500个或更多个核苷酸)的序列。
借助于RNA干扰(RNAi)(实际上，共抑制可以视作一类RNAi)，也可以抑制内源基因表达，所述RNA干扰使用具有与靶基因序列相同或类似的序列的双链RNA。RNAi是这样的现象：其中当将具有与靶基因序列相同或类似的序列的双链RNA引入细胞中时，插入的外源基因和靶内源基因二者的表达被抑制。所述双链RNA可以从2个单独的互补RNA形成，或者可以是具有内部互补序列(其形成双链RNA)的单个RNA。尽管RNAi的机制的整个细节仍然是未知的，认为引入的双链RNA首先被切割成小片段，它们然后以某种方式充当靶基因的索引，由此降解靶基因。已知RNAi在植物中也是有效的(参见，例如，Chuang,C.F.和Meyerowitz,E.M.,Proc.Natl.Acad.Sci.USA 97:4985(2000)；Waterhouse等人,Proc.Natl.Acad.Sci.USA 95:13959-13964(1998)；Tabara等人.Science 282:430-431(1998)；Matthew,Comp Funct Genom 5:240–244(2004)；Lu,等人,Nucleic Acids Res.32(21):e171(2004))。
因而，在某些实施方案中，使用RNAi技术来完成编码木质素或木聚糖生物合成酶的基因的抑制。例如，为了使用RNAi来抑制编码蛋白的DNA的表达，向目标植物中引入双链RNA，其具有编码所述蛋白的DNA的序列或其基本上类似的序列(包括经工程改造成不翻译所述蛋白的那些)或其片段。本文中使用的RNAi和dsRNA都表示，通过双链RNA分子的引入而诱导的基因特异性的沉默(参见例如，美国专利号6,506,559和6,573,099)，并且包括对具有双链区域的分子(例如，短发夹RNA分子)的提及。然后可以针对与靶蛋白有关的表型筛选得到的植物，例如，针对与野生型植物相比得自植物的糖的可提取性增加进行筛选，和/或通过监测编码所述蛋白的转录物的稳态RNA水平。尽管用于RNAi的基因不需要与靶基因完全相同，它们与靶基因序列可以是至少70％、80％、90％、95％或更多相同。参见，例如，美国专利公开号2004/0029283。还可以使用构建体来抑制靶基因表达，所述构建体编码具有茎-环结构的RNA分子，所述茎-环结构与靶基因无关且位于对目标基因特异性的序列的远端。参见，例如，美国专利公开号2003/0221211。
RNAi多核苷酸可以包括全长靶RNA，或者可以与靶RNA的片段对应。在某些情况下，所述片段将具有少于100个、200个、300个、400个、500个、600个、700个、800个、900个或1,000个与靶序列对应的核苷酸。另外，在某些实施方案中，这些片段的长度是至少，例如，50个、100个、150个、200个或更多个核苷酸。在某些情况下，用于RNAi的片段与在所述生物的其它蛋白中不存在的靶蛋白区域至少基本上类似，或者可以选择成与其它生物转录物具有尽可能少的相似性，例如，通过在分析公众可得到的序列数据库时与序列对比来选择。
以瞬时和稳定转染的方式连续表达siRNA的表达载体已经被工程改造成表达小发夹RNA，所述小发夹RNA在体内被加工成能够实现基因特异性沉默的siRNA分子(Brummelkamp等人,Science 296:550-553(2002),和Paddison,等人,Genes&Dev.16:948-958(2002))。Hammond等人.Nature Rev Gen 2:110-119(2001),Fire等人.Nature 391:806-811(1998)和Timmons和Fire Nature 395:854(1998)进一步详细讨论了双链RNA的转录后基因沉默。
抑制内源植物基因表达的另一种方式是，通过抑制靶标(例如，编码木质素或木聚糖生物合成酶的基因)的微RNA的重组表达。人工微RNA是单链RNA(例如，18-25聚体，通常21聚体)，其通常不存在于植物中，并且从内源miRNA前体加工得到。根据植物miRNA靶标选择的决定簇来设计它们的序列，使得人工微RNA会特异性地沉默它的目标靶基因，并且一般地描述在Schwab等人,The Plant Cell 18:1121-1133(2006)中，以及基于因特网的设计其中描述的这类微RNA的方法。也参见,美国专利公开号2008/0313773。
降低一个或多个目标基因的基因表达产物水平的方法的另一个例子采用核糖开关技术(参见，例如，美国专利申请公开号US20100286082,和US20110245326)。
在本领域中已经描述了抑制植物的一种或多种木质素和/或木聚糖生物合成酶的基因表达的方法，包括具有受抑制的RWA表达的植物。参见，例如，Coleman等人,PlantPhysiol.148:1229-37(2008)(杨树中的C3'H RNAi)；Kitin等人,Plant Physiol.154:887-98(2010)(杨树中的4CL反义)；Coleman等人,Proc.Acad.Natl.Sci.USA 105:4501-06(2008)(杨树中的C3'H RNAi)；和Voelker等人,Plant Physiol.154:874-86(2010)(杨树中的4CL反义),和WO2010/096488(RWA抑制)，它们中的每一篇通过引用整体并入本文。
本领域技术人员会明白，靶向了在木质部和纤维中高度表达的异形体。例如，使用拟南芥属用于例证目的，IRX7、IRX8、IRX9、PARVUS、IRX15在木质部和纤维中高度表达，并因此被靶向。对于IRX10和IRX14，这两种异形体(拟南芥属具有2种异形体)通常被靶向，因为它们二者在木质部和纤维中表达。类似地，为了制备其Rwa表达受到抑制的植物，靶向在木质部和纤维中表达的异形体。例如，再次使用拟南芥属来例证，RWA1、RWA3和RWA4之一(通常2个或更多个)被靶向(RWA2不在木质部和纤维中表达)。
本领域进一步理解，在本发明方法的使用导管特异性的启动子(例如VND6)将活性引回木聚糖缺陷型或木质素缺陷型植物的步骤中，不一定表达与为了抑制而靶向的异形体相同的异形体。例如，可以采用具有极少木聚糖的irx9突变体植物，但是不一定在该植物中表达组织特异性的IRX9异形体，相反，还可以容易地采用在那些组织中通常不表达的IRX9同系物。许多植物(包括拟南芥属)具有第二个IRX9-like基因，其主要在除了木质部和纤维以外的组织中表达。类似的关联对于IRX7/F8H、IRX14/IRX14-like和IRX15/IRX15-like也是真实的。同样地，可以工程改造RWA1/RWA3/RWA4突变体以在导管特异性的启动子(例如，VND6启动子)控制下表达Rwa2。
b)具有突变体背景的植物
在某些实施方案中，通过制备具有在编码木质素或木聚糖生物合成酶的基因中的突变的植物，降低木质素或木聚糖生物合成酶的表达水平。用于消除或减少编码木质素或木聚糖生物合成酶的基因的表达的一种方法是，通过使用根瘤土壤杆菌的T-DNA的插入诱变。在制备插入突变体以后，可以筛选突变体以鉴别在目标基因中含有插入的那些。可以杂交含有在目标基因处的单突变事件的突变体，以制备所述突变的纯合植物(Koncz等人(1992)Methods in Arabidopsis Research.World Scientific)。
可替换地，可以使用随机诱变方案，以制备将产生截短的或有缺陷的(无功能的或活性较差的)酶或不稳定的RNA的新等位基因，或者以破坏或“敲除”编码木质素或木聚糖生物合成酶的基因的表达(其中使用化学或插入诱变或辐照)。诱变和突变体鉴别的一种方法被称作TILLING(用于靶向基因组中的诱导的局部损伤)。在该方法中，在目标植物的种子中诱导突变，例如，使用EMS处理。培养得到的植物并自我授粉，并评估后代。例如，可以如下评估植物：使用PCR来鉴别突变的植物是否具有在目标基因中的突变，或通过评价所述植物是否在表达目标基因的植物部分中具有降低的木质素含量。TILLING可以鉴别出这样的突变：其可能改变特定基因的表达或由这些基因编码的蛋白的活性(参见Colbert等人(2001)Plant Physiol 126:480-484；McCallum等人(2000)Nature Biotechnology 18:455-457)。
在本领域中已经描述了制备特定植物的方法，所述植物具有一种或多种木质素和/或木聚糖生物合成酶的突变体背景。参见，例如，Schilmiller等人,Plant J.60:771-82(2009)(C4H的拟南芥属突变体)；和Weng等人,Plant Cell 22:1033-45(2010)(F5H的卷柏属突变体)，它们中的每一篇通过引用整体并入本文。制备具有RWA突变体背景的植物的方法已经描述在例如WO2010/096488中。
在某些要将包含木质素生物合成酶和木聚糖生物合成酶的表达盒引入植物中的实施方案中，所述植物具有这样的遗传背景：其被修饰成具有降低的木质素生物合成酶和木聚糖生物合成酶表达水平。这样的植物可以使用本文的应用部分中描述的已知方法来制备，所述应用部分描述了修饰植物以抑制或减少目标产物的表达。
B.使用调节次生细胞壁产生的转录因子来修饰表达
在另一个方面，本发明提供了工程改造植物的方法，所述植物具有增加的次生细胞壁沉积。在某些实施方案中，所述方法包括：
将表达盒引入植物中，其中所述表达盒包含编码转录因子的多核苷酸，所述转录因子调节次生细胞壁在木质组织中的生产量，所述多核苷酸与诱导型异源启动子可操作地连接，其中所述启动子与一定基因的天然启动子基本上相同，所述一定基因在生物合成途径中是所述转录因子的下游靶标；和
在表达所述转录因子的条件下，培养所述植物。所述下游靶标可以是所述转录因子的直接或间接靶标。
当被引入植物中时，在本文中描述的表达盒会产生正反馈回路，其允许维持在次生细胞壁生物合成中涉及的基因的表达或过表达，因为所述转录因子会直接地或间接地诱导所述下游靶基因的启动子驱动的表达，所述启动子又与编码所述转录因子的多核苷酸可操作地连接，从而导致增加的转录因子表达。该正反馈回路会导致次生细胞壁组分(诸如纤维素、半纤维素和木质素)的持续产生或过度产生。
1.调节次生细胞壁产生的转录因子
在某些实施方案中，所述表达盒包含编码转录因子的多核苷酸，所述转录因子调节次生细胞壁产生。基于它诱导在木质素生物合成和/或多糖(纤维素和半纤维素)生物合成中涉及的一个或多个基因，可以选择用于本发明中的转录因子。可替换地或额外地，基于在植物中的过表达或功能缺失表型(例如，过表达表现出增加的细胞壁增厚或次生细胞壁沉积表型的转录因子的植物，或具有表现出减少的细胞壁增厚或次生细胞壁沉积表型的转录因子的显性抑制或功能缺失突变的植物)，可以选择使用的转录因子。在某些实施方案中，所述转录因子是NAC次生壁增厚促进因子1(NST1)、NST2、NST3、次生壁相关的NAC结构域蛋白2(SND2)、SND3、MYB结构域蛋白103(MYB103)、MBY85、MYB46、MYB83、MYB58或MYB63。
已经在拟南芥属中表征了转录因子NST1、NST2、NST3、SND2、SND3、MYB103、MBY85、MYB46、MYB83、MYB58和MYB63，并且已经证实它们会调节该物种中的次生细胞壁产生。参见，例如，Mitsuda等人,Plant Cell 17:2993-3006(2005)；Mitsuda等人,Plant Cell 19:270-80(2007)；Ohashi-Ito等人,Plant Cell 22:3461-73(2010)；Zhong等人,Plant Cell 20:2763-82(2008)；Zhong等人,Plant Cell 19:2776-92(2007)；Ko等人,Plant J.60:649-65(2009)；和McCarthy等人,Plant Cell Physiol.50:1950-64(2009)。因而，在某些实施方案中，编码调节次生细胞壁产生的转录因子的多核苷酸与SEQ ID NO:13、15、17、19、21、23、25、27、29、31或33中的任一个多核苷酸序列基本上相同。另外，已经在多种其它植物中鉴别出这些转录因子，所述植物包括水稻、高粱、杨树、葡萄、藓类植物、玉米和柳枝稷。此外，次生细胞壁生物合成的一般机制不仅在单子叶植物和双子叶植物之间是保守的，而且在这些组内也是保守的。因而，在某些实施方案中，编码调节次生细胞壁产生的转录因子的多核苷酸包含下述序列的同系物：SEQ ID NO:13、15、17、19、21、23、25、27、29、31或33中的任一个多核苷酸序列，SEQ ID NO:14、16、18、20、22、24、26、28、30、32或34中的任一个氨基酸序列，或图13中的任意氨基酸序列。
在某些实施方案中，编码调节次生细胞壁在木质组织中的生产量的转录因子的多核苷酸包含，与SEQ ID NO:13、15、17、19、21、23、25、27、29、31或33中的任一个基本上相同(例如，至少50％、至少55％、至少60％、至少65％、至少70％、至少75％、至少80％、至少85％、至少90％、至少91％、至少92％、至少93％、至少94％、至少95％、至少96％、至少97％、至少98％或至少99％同一)的多核苷酸序列。在某些实施方案中，编码调节次生细胞壁在木质组织中的生产量的转录因子的多核苷酸包含这样的多核苷酸序列：其编码与SEQ ID NO:14、16、18、20、22、24、26、28、30、32或34中的任一个基本上相同(例如，至少50％、至少55％、至少60％、至少65％、至少70％、至少75％、至少80％、至少85％、至少90％、至少91％、至少92％、至少93％、至少94％、至少95％、至少96％、至少97％、至少98％或至少99％同一)的多肽序列。在某些实施方案中，所述调节木质中的次生细胞壁产生的转录因子包含这样的氨基酸序列：其与SEQ ID NO:14、16、18、20、22、24、26、28、30、32或34中的任一个或与图13中的任一个氨基酸序列基本上相同(例如，至少50％、至少55％、至少60％、至少65％、至少70％、至少75％、至少80％、至少85％、至少90％、至少91％、至少92％、至少93％、至少94％、至少95％、至少96％、至少97％、至少98％或至少99％同一)。
在本文的序列表中描述了NST1、NST2、NST3、SND2、SND3、MYB103、MBY85、MYB46、MYB83、MYB58和MYB63的基因和蛋白序列和/或登录号。另外，在图1-6中显示了所述转录因子的氨基酸序列比对，其中显示了得自多个植物种的这些蛋白中的每一种的氨基酸序列。在本领域中也已知并描述了这些蛋白的基因和蛋白序列以及获得所述基因或蛋白的方法。参见，例如，Goiocoechea等人,2005,Plant J.43:553-67；McCarthy等人,2009,Plant CellPhysiol.50:1950-64；Shen等人,2009,Bioenerg.Res.2:217-32；和Zhong等人,2010,Trends in Plant Sciences,http://dx.doi.org/10.1016/j.tplants.2010.08.007。本领域技术人员会认识到，可以修饰本领域中已知的和/或在本文中描述的这些基因或蛋白序列以制备基本上相同的转录因子，例如，通过在一个或多个氨基酸残基处产生保守置换。技术人员还会认识到，已知的序列(例如，本文中提供的比对)会提供关于可以改变哪个氨基酸来制备基本上相同的转录因子的指导。例如，使用图1-6所示的任意比对，技术人员会认识到哪个氨基酸残基不是高度保守的，并因而可能进行改变，而不对所述转录因子的功能产生显著影响。
2.作为调节次生细胞壁产生的转录因子的下游靶标的启动子
在某些实施方案中，所述编码调节次生细胞壁产生的转录因子的多核苷酸与作为所述转录因子的下游靶标的启动子可操作地连接。所述启动子相对于编码调节次生细胞壁产生的转录因子的多核苷酸而言是异源的(即，不是与调节次生细胞壁产生的转录因子有关的天然启动子)。在下述情况下，启动子适合与调节次生细胞壁产生的转录因子一起使用：如果所述启动子的表达直接地或间接地被要表达的转录因子诱导，和如果所述启动子在目标位置(例如，植物的茎)表达，但是在植物的叶子中不强烈表达。
在某些实施方案中，所述启动子与作为所述转录因子的下游靶标的基因的天然启动子基本上相同(例如，至少50％、至少55％、至少60％、至少65％、至少70％、至少75％、至少80％、至少85％、至少90％、至少91％、至少92％、至少93％、至少94％、至少95％、至少96％、至少97％、至少98％或至少99％同一)。在某些实施方案中，所述启动子与IRX1、IRX3、IRX5、IRX8、IRX9、IRX14、IRX7或IRX10的天然启动子基本上相同。在某些实施方案中，所述转录因子选自NST1、NST2、NST3、SND2、SND3、MYB103、MBY85、MYB46、MYB83、MYB58和MYB63，且所述启动子与选自IRX1、IRX3、IRX5、IRX8、IRX9、IRX14、IRX7、IRX10、GAUT13或GAUT14的天然启动子基本上相同。参见图14。还可以使用替代启动子。例如，可以如下鉴别替代启动子：通过共表达分析，例如，使用Atted II数据库和已知的启动子作为诱饵；或通过鉴别候选基因的启动子中的目标功能基序。还可以使用受所述转录因子调节的其它基因的启动子。
在某些实施方案中，所述启动子包含SEQ ID NO:35的子序列或其变体。在某些实施方案中，所述启动子包含SEQ ID NO:35的子序列，所述子序列包含SEQ ID NO:35的约50个至约1000个或更多个连续核苷酸。在某些实施方案中，所述启动子包含SEQ ID NO:35的子序列，所述子序列包含SEQ ID NO:35的50-1000个、50-900个、50-800个、50-700个、50-600个、50-500个、50-400个、50-300个、50-200个、50-100；75-1000个、75-900个、75-800个、75-700个、75-600个、75-500个、75-400个、75-300个、75-200；100-1000个、100-900个、100-800个、100-700个、100-600个、100-500个、100-400个、100-300个或100-200个连续核苷酸。
在本领域中还描述了作为本文所述的转录因子的下游靶标的启动子。参见，例如，Oikawa等人,2010,PLoS ONE；Taylor等人,2000,Plant Cell；Betancur等人,2010,J.Integrative Plant Biol.；Persson等人,2007,Plant Physiol.；Wu等人,2010,PlantPhysiol.；Zhong等人,2005,Plant Cell；和Wu等人,2009,Plant J.；它们中的每一篇通过引用整体并入本文。
本领域技术人员会明白，启动子区域可以耐受显著的变异而不减少活性。因而，在某些实施方案中，所述启动子与SEQ ID NO:35基本上相同(例如，至少50％、至少55％、至少60％、至少65％、至少70％、至少75％、至少80％、至少85％、至少90％、至少91％、至少92％、至少93％、至少94％、至少95％、至少96％、至少97％、至少98％或至少99％同一)。
C.使用调节蜡/角质产生的转录因子来修饰表达
为了减少植物固定每吨CO2的水消耗和提高植物干旱胁迫耐受性，提高的植物水利用效率是一个重要的首选因素。通过减少细胞的氧化性应激，将会改善或维持在水限制条件下的生物质产量，这也会造成光合作用效率的下降。开发出可以减少植物的水损耗且不减少生物质产量的策略，会减少水需求，提高干旱胁迫耐受性，并且与已经开发的干旱胁迫耐受性技术相容。植物损耗的水的一部分是通过在叶表皮表面上的角质层(也称为上角质层)的水蒸发而发生。已经鉴别出了控制蜡/角质生物合成的转录因子。尽管这些转录因子中的一些在植物中的过表达会提高对干旱胁迫的抗性和降低水损耗，用于增加这些转录因子的表达的表达策也会造成蜡或/和角质在敏感组织中的沉积，从而对植物生长和发育产生不希望的作用(Aharoni等人,The Plant Cell 16:2463-2480,2004；Zhang等人,PlantJ.42:689-797,2005)。除了水利用效率以外，修饰上角质层的蜡组成和含量具有几个其它潜在优点，因为上角质层是许多病原体、昆虫和化学试剂的第一屏障。因此，本发明提供了一种人工正反馈回路系统，其用于增加植物表皮上的蜡和/或角质沉积，以便提高植物水利用效率和干旱胁迫耐受性。
因而，在另一个方面，本发明提供了工程改造植物的方法，所述植物具有改变的(例如，增加的)蜡和/或角质产生。在某些实施方案中，所述方法包括：
将表达盒引入植物中，其中所述表达盒包含编码转录因子的多核苷酸，所述转录因子调节蜡/角质组分的产生，所述多核苷酸与异源诱导型启动子连接，其中所述启动子与一定基因的天然启动子基本上相同，所述一定基因是所述转录因子的下游靶标；和
在表达所述转录因子的条件下，培养所述植物。所述下游靶标可以是所述转录因子的直接或间接靶标。
当被引入植物中时，在本文中描述的表达盒会产生正反馈回路，其允许维持在蜡和/或角质生物合成中涉及的基因的表达或过表达，因为所述转录因子会直接地或间接地诱导所述下游靶基因的启动子驱动的表达，所述启动子又与编码所述转录因子的多核苷酸可操作地连接，从而导致增加的转录因子表达。该正反馈回路会导致蜡和/或角质的持续产生或过度产生。
1.调节蜡/角质产生的转录因子
在某些实施方案中，所述表达盒包含编码转录因子的多核苷酸，所述转录因子调节用于生产蜡(和/或角质)的蜡和/或角质组分的产生。基于它诱导在蜡生物合成途径中涉及的一个或多个基因(通常多个基因)，可以选择用于本发明中的转录因子。可替换地或额外地，可以基于在植物中的过表达或功能缺失表型来选择使用的转录因子(例如，过表达表现出增加的蜡生产量表型的转录因子的植物，或具有表现出减少的蜡生产量表型的转录因子的显性抑制或功能缺失突变的植物)。在某些实施方案中，所述转录因子是shine(SHN)转录因子，诸如SHN1(也被称作WIN1)、SHN2、SHN3、SHN4、SHN5或MYB 96。
已经在拟南芥属中表征了转录因子SHN1、SHN2、SHN3、SHN4、SHN5和MYB96，并且已经证实它们会在拟南芥属和其它植物种中调节蜡和/或角质生物合成。参见，例如，Shi等人,PLoS Genet.7,e1001388(2011)；Seo等人,Plant Cell 23:1138-1152(2011)；Kannangara等人,Plant Cell 19:1278-1294(2007)；Zhang等人,Plant J.42:689-707(2005),Aharoni等人,Plant Cell 16:2463-2480(2004)；Broun等人,Proc.Natl.Acad SciUSA 101:4706-4711(2004)；和Suh等人,Plant Physiol.139:1649-1665(2005)。另外，已经在多种其它植物中鉴别出了SHN转录因子序列，所述植物包括杨树、苜缩属、水稻、草例如短柄草属、玉米、高粱、大麦、云杉、卷柏和苔藓植物。类似地，已经在多种其它植物中鉴别出了Myb96转录因子序列，所述植物包括盐芥属、苜缩属、杨树、葡萄藤、柑橘、短柄草属、小麦、大麦、水稻和高粱。此外，蜡/角质生物合成的一般机制不仅在单子叶植物和双子叶植物之间是保守的，而且在这些组内也是保守的。
在某些实施方案中，编码调节蜡/角质产生的转录因子的多核苷酸会编码SHN转录因子。在某些实施方案中，所述多核苷酸编码SEQ ID NO:37-59中的任一个的SHN转录因子或其变体。因而，在某些实施方案中，编码调节蜡/角质合成生产量的转录因子的多核苷酸会编码与SEQ ID NO:37-59中的任一个基本上相同的蛋白。
在某些实施方案中，编码调节蜡角质合成生产量的转录因子的多核苷酸包含这样的多核苷酸序列：其编码与SEQ ID NO:37-59中的任一个具有至少50％、至少55％、至少60％、至少65％、至少70％、至少75％、至少80％、至少85％、至少90％、至少91％、至少92％、至少93％、至少94％、至少95％、至少96％、至少97％、至少98％或至少99％同一性的氨基酸序列。
在某些实施方案中，编码调节蜡/角质产生的转录因子的多核苷酸会编码Myb96转录因子。在某些实施方案中，所述多核苷酸会编码SEQ ID NO:80-93中的任一个的Myb96转录因子或其变体。因而，在某些实施方案中，编码调节蜡/角质合成生产量的转录因子的多核苷酸会编码与SEQ ID NO:80-93中的任一个基本上相同的蛋白。
在某些实施方案中，编码调节蜡角质合成生产量的转录因子的多核苷酸包含这样的多核苷酸序列：其编码与SEQ ID NO:80-93中的任一个具有至少50％、至少55％、至少60％、至少65％、至少70％、至少75％、至少80％、至少85％、至少90％、至少91％、至少92％、至少93％、至少94％、至少95％、至少96％、至少97％、至少98％或至少99％同一性的氨基酸序列。
在本文中提供了SHN1、SHN2、SHN3、SHN4、SHN5或MYB 96的示例性的蛋白序列和/或登录号。另外，在图25和26中显示了所述转录因子的氨基酸序列比对，其中显示了得自多个植物种的这些蛋白中的每一种的氨基酸序列,。在本领域中已知并描述了这些蛋白的基因和蛋白序列以及获得所述基因或蛋白的方法(参见，例如，在上文中引用的参考文献)。本领域技术人员会认识到，可以修饰本领域中已知的和/或在本文中描述的这些基因或蛋白序列以制备变体转录因子，例如，通过在一个或多个氨基酸残基处产生保守置换。技术人员还会认识到，已知的序列(例如，本文中提供的比对)会提供关于可以改变哪个氨基酸来制备基本上相同的转录因子的指导。例如，使用在图25和26中提供的比对，技术人员会认识到哪个氨基酸残基不是高度保守的，并因而可能进行改变，而不对所述转录因子的功能产生显著影响。类似地，技术人员可以鉴别在所有或几乎所有的转录因子中保守的高度保守的结构域，并在鉴别用于本发明中的变体中使用该信息。
2.作为调节蜡和/或角质产生的转录因子的下游靶标的启动子
在某些实施方案中，所述编码调节蜡和/或角质产生的转录因子的多核苷酸与作为所述转录因子的下游靶标的启动子可操作地连接。所述启动子相对于编码调节蜡和/或角质产生的转录因子的多核苷酸而言是异源的(即，不是与所述转录因子有关的天然启动子)。在下述情况下，启动子适合与所述转录因子一起使用：如果所述启动子的表达直接地或间接地被要表达的转录因子诱导，和如果所述启动子在植物中的目标位置(例如，植物的叶)表达。
在某些实施方案中，所述启动子与作为所述转录因子的下游靶标的基因的天然启动子基本上相同(例如，至少50％、至少55％、至少60％、至少65％、至少70％、至少75％、至少80％、至少85％、至少90％、至少91％、至少92％、至少93％、至少94％、至少95％、至少96％、至少97％、至少98％或至少99％同一)。在某些实施方案中，所述启动子是CER1、CER2、CER3、CER4、CER5、CER6、CER10、WSD1、Mah1、WBC11、KCS1、KCS2、FATB、LACS1、LACS2、CYP864A、CYP86A7、CYP86A5、KCS10或KCS5启动子，或它们的与天然启动子基本上相同的变体。在某些实施方案中，所述转录因子选自SHN1、SHN2、SHN3、SHN4、SHN5或MYB 96，且所述启动子与选自CER1、CER2、CER3、CER4、CER5、CER6、CER10、WSD1、Mah1、WBC11、KCS1、KCS2、FATB、LACS1、LACS2、CYP864A、CYP86A7、CYP86A5、KCS10或KCS5的天然启动子基本上相同。还可以使用替代启动子。例如，可以如下鉴别替代启动子：通过共表达分析，例如，使用Atted II数据库和已知的启动子作为诱饵；或通过鉴别候选基因的启动子中的目标功能基序。还可以使用受所述转录因子调节的其它基因的启动子。
在某些实施方案中，所述启动子包含SEQ ID NO:60-79中的任一个的子序列(例如，得自WBC11或CER1的序列)或其变体。在某些实施方案中，所述启动子包含SEQ ID NO:60-79中的任一个的子序列，所述子序列包含约50个至约1000个或更多个连续核苷酸。在某些实施方案中，所述启动子包含SEQ ID NO:60-79中的任一个的子序列，所述子序列包含所述序列的50-1000个、50-900个、50-800个、50-700个、50-600个、50-500个、50-400个、50-300个、50-200个、50-100；75-1000个、75-900个、75-800个、75-700个、75-600个、75-500个、75-400个、75-300个、75-200个、100-1000个、100-900个、100-800个、100-700个、100-600个、100-500个、100-400个、100-300个或100-200个连续核苷酸。
在本领域中还描述了作为本文所述的转录因子的下游靶标的启动子。参见，例如，植物中的蜡生物合成的综述和其中引用的参考文献(Schreiber,Trends Plant Sci.,2010；Kunst和Samuels,Curr.Opinion Plant Biol.12:721-727,2009；Samuels等人,Annu.Rev.Plant Biol.59:683-707,2008；Nawrath,I9:281-287,2006；Kunst和Samuels,Progress in Lipid Res.42:51-80,2003；Lemieux,Trends in Plant Sci.1:312,1996)。描述在拟南芥属中分析的蜡突变体的参考文献包括：Bourdenx等人,Plant Physiol 156,29–45(2011)；Panikashvili等人.Mol Plant 3,563–575(2010)；Weng,等人,Planta 231,1089–1100(2010)；Lee等人.Plant J 60,462–475(2009)；Li等人,Plant Physiol 148,97–107(2008)；Greer等人,Plant Physiol 145,653–667(2007)；Rowland等人,FEBS Lett581,3538–3544(2007)；Rowland等人,Plant Physiol 142,866–877(2006)；Costaglioli等人,Biochim Biophys Acta 1734,247–258(2005)；Sturaro等人,Plant Physiol 138,478–489(2005)；Schnurr等人,Plant Cell 16,629–642(2004)；Pighin等人,Science 306,702–704(2004)；Bonaventure等人,Plant Cell 15,1020–1033(2003)；Chen等人,Plant Cell15,1170–1185(2003)；Fiebig等人,Plant Cell 12,2001–2008(2000)；和Millar等人,Plant Cell 11,825–838(1999)。蜡生物合成途径在植物物种之间也是保守的(参见，例如，Wang等人,Plant Mol Biol 78,275–288(2011)；Mao等人,Planta 235,39–52(2012)；Yu等人,Planta 228,675–685(2008)；Tacke等人,Plant J 8,907–917(1995)；Islam等人,PlantMol Biol 70,443–456(2009)；Post-Beittenmiller Plant Physiol Bioch 36,157–166(1998)；和Park等人,Plant Mol Biol 74,91–103(2010))。
D.人工正反馈回路
在另一个方面，本发明提供了用于调节植物中的基因表达的人工正反馈回路。APFL会过度诱导或增加特定转录因子和它的下游途径的终生表达。在上面关于纤维茎中的次生壁沉积和关于蜡沉积描述了这样的系统的例子。在图27和28中显示了作为该策略的基础原理的细胞壁致密化和蜡沉积的示例性例子。适合用于APFL中的转录因子通常在控制目标途径的多种组分的表达中起作用。使用细胞类型特异性的启动子(其中由转录因子驱动表达)作为APFL构建体中的启动子。如下建立APFL：将表达构建体引入植物细胞中，其中所述构建体包含编码目标转录因子的多核苷酸，所述多核苷酸与期望的启动子可操作地连接。在表达天然转录因子后，与由APFL构建体编码的引入的转录因子的表达一起诱导下游基因的表达。
可以采用APFL的生物合成途径的其它例子包括脂质生物合成途径。例如，已知的是，在种子和其它组织中的脂质生物合成和积累发生在特定细胞类型中，并由诸如WRL1(WRINKLED；At3g54320)、LEC1(At1g21970)或LEC2(At1g28300)等转录因子调节。因此，可以使用这些转录因子来建立AFPL以增加脂质在期望的组织(诸如种子)中的积累。还可以为其它生物合成途径鉴别出用于APFL中的其它转录因子和适当启动子。在例如下述文献中讨论了脂质生物合成途径：Ohlrogge和Browse,Plant Cell 7:957,1995；Hildebrand,等人,Plant Lipids:Biology,Utilisation and Manipulation,67-102(2005)；和Dyer&Mullen,Seed Sci.Res.15:255-267(2005)。
可以进行工程改造以建立APFL的其它生物合成途径包括萜类化合物途径。例如，可以建立APFL以增加萜类吲哚生物碱生物合成。可以用于这样的APFL的转录因子包括CrMYC2、ORCA2或ORCA3。可以将编码所述转录因子的核酸与诱导型启动子(诸如pSTR)可操作地连接，所述诱导型启动子控制异胡豆苷合酶从长春花(catharanthus roseus)的表达。萜类吲哚生物碱途径是已知的(参见，例如，Peebles,等人,Metab Eng 11:76–86(2009)；Liu,等人,J Integr Plant Biol 49:961–974(2007)；Menke,等人,.EMBO J 18:4455–4463(1999)，所述参考文献各自通过引用并入)。
APFL的另一个例子是用于增加青蒿素(倍半萜)生物合成的APFL。可以用于这样的APFL的示例性转录因子是AaWRK1(得自黄花蒿)。可以将编码所述转录因子的核酸与诱导型启动子(诸如pADS)可操作地连接，所述诱导型启动子控制紫穗槐-4,11-二烯合酶从黄花蒿的表达。该生物合成途径是已知的(参见，例如，Ma,等人,Plant Cell Physiol 50:2146–2161(2009)，其通过引用并入)。
APFL的另一个例子是用于增加小檗碱(一种生物碱)生物合成的APFL。可以用于这样的APFL的示例性转录因子是CjWRK1(得自日本黄连)。可以将编码所述转录因子的核酸与诱导型启动子(诸如pCYP719A1)可操作地连接，所述诱导型启动子控制四氢小檗碱合酶从日本黄连的表达。该生物合成途径是已知的(参见，例如，Kato,等人,Plant Cell Physiol488–18(2007)，其通过引用并入)。
E.在其中引入人工反馈回路的植物的遗传背景
在某些实施方案中，在其中表达如本文所述的编码转录因子的多核苷酸的植物是野生型(即，天然存在的)植物，所述多核苷酸与下游基因的启动子连接，其中表达由所述转录因子驱动。在某些实施方案中，在其中表达如本文所述的编码转录因子的多核苷酸的植物是突变体植物。本文中使用的“突变体植物”包括：具有任意一个或多个目标基因的任何功能缺失或功能获得突变的植物，以及在其中使用已知方法(例如，通过反义、siRNA、微RNA、dsRNA或有义抑制)抑制或减少任意一个或多个目标基因的内源表达的植物或。例如，在某些实施方案中，使用已知的技术诸如核糖开关技术(参见，例如，美国专利申请公开号US20100286082,和US20110245326)，可以减少一个或多个目标基因的基因表达产物水平。
在某些实施方案中，在其中表达如本文所述的编码转录因子的多核苷酸的植物是这样的植物：其具有如上所述的木质素生物合成酶和/或木聚糖生物合成酶的经空间修饰的基因表达。在某些实施方案中，所述植物已经被修饰成至少在除了木质部组织以外的组织中具有降低的木质素生物合成酶表达水平和/或木聚糖生物合成酶，且另外包含表达盒，所述表达盒包含编码木质素生物合成酶(例如，PAL、C4H、4CL、HCT、C3'H或CCR1)和/或木聚糖生物合成酶(例如，IRX8、IRX14、IRX9、IRX7、IRX10、F8H、PARVUS、RWA1、RWA2、RWA3或RWA4)的多核苷酸，所述多核苷酸与异源的导管特异性的启动子(例如，pVND1、pVND2、pVND3、pVND4、pVND5、pVND6、pVND7、pVNI2、pREF4或pRFR1)可操作地连接。
F.重组表达载体的制备
得到启动子序列和目标基因的编码序列(例如，木质素生物合成酶、木聚糖生物合成酶、或调节次生细胞壁产生的转录因子)后，可以使用所述序列来制备用于在转基因植物中表达目标基因的表达盒。通常，植物转化载体包括一个或多个克隆的植物编码序列(基因组或cDNA)，所述编码序列编码目标蛋白(诸如转录因子)，并在5'和3'调节序列的转录控制下。载体也通常包含显性选择标记。在典型实施方案中，这样的植物转化载体也含有目标启动子(例如，本文所述的导管特异性的启动子或其表达受调节次生细胞壁产生的转录因子调节的启动子)、转录起始起始位点、RNA加工信号(诸如内含子剪接位点)、转录终止位点、和/或多腺苷酸化信号。
所述植物表达载体可以包括RNA加工信号，其可以位于编码序列的内部、上游或下游。另外，所述表达载体可以包括植物基因的3'-非翻译区的调节序列，例如，增加mRNA的mRNA稳定性的3'终止子区域，诸如马铃薯的PI-II终止子区域或章鱼碱或胭脂碱合酶3'终止子区域。
植物表达载体常规地也包括显性选择标记基因以允许容易地选择转化体。这样的基因包括编码抗生素抗性基因(例如，对潮霉素、卡那霉素、博来霉素、G418、链霉素或大观霉素的抗性)的那些基因、除草剂抗性基因(例如，草胺膦乙酰基转移酶)和编码阳性选择酶(例如甘露糖异构酶)的基因。
一旦已经构建出本文所述的包含多核苷酸的表达盒，所述多核苷酸编码木质素生物合成酶、木聚糖生物合成酶、或调节次生细胞壁产生的转录因子，并且与启动子可操作地连接，就可以使用标准技术来将所述多核苷酸引入植物中，以便修饰基因表达。参见，例如，在下述文献中描述的方案：Ammirato等人(1984)Handbook of Plant Cell Culture--CropSpecies.Macmillan Publ.Co.Shimamoto等人(1989)Nature 338:274-276；Fromm等人(1990)Bio/Technology 8:833-839；和Vasil等人(1990)Bio/Technology 8:429-434。
植物的转化和再生是本领域已知的，并且最适当的转化技术的选择将由从业人员确定。合适的方法可以包括、但不限于：植物原生质体的电穿孔；脂质体介导的转化；聚乙二醇(PEG)介导的转化；使用病毒的转化；植物细胞的显微注射；植物细胞的微粒轰击；真空渗入；和根瘤土壤杆菌介导的转化。转化是指，以特定方式将核苷酸序列引入植物中，以造成所述序列的稳定或瞬时表达。在不同植物中的这些方法的例子包括：美国专利号5,571,706、5,677,175、5,510,471、5,750,386、5,597,945、5,589,615、5,750,871、5,268,526、5,780,708、5,538,880、5,773,269、5,736,369和5,610,042。
转化后，使用掺入转化载体中的显性选择标记，可以选择植物。通常，这样的标记会给转化的植物赋予抗生素或除草剂抗性或在特定底物上生长的能力，并且可以通过将所述植物暴露于适当浓度的抗生素、除草剂或底物来完成转化体的选择。
根据本领域已知的任意方法，可以得到编码木质素生物合成酶、木聚糖生物合成酶或调节次生细胞壁产生的转录因子的多核苷酸，以及包含导管特异性启动子或作为调节次生细胞壁产生的转录因子的下游靶标的启动子的启动子序列的多核苷酸。这样的方法可以包括扩增反应诸如PCR和其它基于杂交的反应，或者可以直接合成。
G.在其中可以修饰基因表达的植物
可以在不同种类的植物中表达如本文所述的包含多核苷酸的表达盒，所述多核苷酸包含编码木质素生物合成酶、木聚糖生物合成酶、或调节次生细胞壁产生的转录因子，且与启动子可操作地连接。所述植物可以是单子叶植物或双子叶植物。在本发明的某些实施方案中，所述植物是绿色田野植物。在某些实施方案中，所述植物是裸子植物或针叶树。
在某些实施方案中，所述植物是适合用于制备生物质的植物。合适的植物的例子包括、但不限于：拟南芥属、杨树、桉树、水稻、玉米、柳枝稷、高粱、粟、芒属、甘蔗、松树、苜蓿、小麦、大豆、大麦、草坪草、烟草、大麻、竹、油菜、向日葵、柳树、麻风树属和短柄草属。
在某些实施方案中，在其中引入表达盒的植物是与所述启动子相同的植物物种，和/或与编码木质素生物合成酶、木聚糖生物合成酶、或转录因子的多核苷酸相同的植物物种(例如，在拟南芥属植物中表达得自拟南芥属的导管特异性的启动子、木质素生物合成酶、木聚糖生物合成酶、和/或转录因子)。在某些实施方案中，在其中引入表达盒的植物是与所述启动子不同的植物物种，和/或与编码木质素生物合成酶、木聚糖生物合成酶、或转录因子的多核苷酸不同的植物物种(例如，在杨树植物中表达得自拟南芥属的导管特异性的启动子、木质素生物合成酶、木聚糖生物合成酶、和/或转录因子)。参见，例如，McCarthy等人,Plant Cell Physiol.51:1084-90(2010)；和Zhong等人,Plant Physiol.152:1044-55(2010)。
H.筛选具有修饰的基因表达的植物
在选择转化的植物以后，可以评价所述植物或植物部分以确定一个或多个目标基因的表达谱是否已经被修饰，例如，通过评价RNA或蛋白的水平，通过评价植物或植物部分中的木质素含量、木聚糖含量和/或次生细胞壁沉积量，或通过确定可以从所述植物提取的可溶性糖的量。这些分析可以使用本领域已知的任意数目的方法来实现。
在某些实施方案中，通过评价RNA或蛋白的水平来筛选植物。测量RNA表达的方法是本领域已知的，且包括，例如，PCR、RNA印迹分析、逆转录酶聚合酶链式反应(RT-PCR)和微阵列。测量蛋白水平的方法也是本领域已知的，且包括，例如，质谱法或基于抗体的技术诸如ELISA、蛋白质印迹法、流式细胞计量术、免疫荧光和免疫组织化学。
在某些实施方案中，通过评价木质素含量、木聚糖含量和/或次生细胞壁沉积的量来筛选植物。例如，通过分光光度法、显微术、克拉松木素测定、乙酰基溴试剂或通过组织化学染色(例如，用间苯三酚)，可以评估木质素含量。例如，通过免疫组织化学(例如，用LM10单克隆抗体)，可以评估木聚糖含量。例如，通过组织化学染色(例如，间苯三酚或Maule试剂)或酶促或化学反应(例如，多糖水解或TFA水解)，可以评估次生细胞壁沉积量。
IV.使用具有经空间修饰的基因表达的植物的方法
得自具有木质素生物合成酶、木聚糖生物合成酶和/或调节次生细胞壁产生的转录因子中的一种或多种的经空间修饰的基因表达的植物的植物、植物部分或植物生物质材料可以用于多种方法。在某些实施方案中，所述植物、植物部分或植物生物质材料被用于转化反应中以制备与野生型植物相比增加的量的生物能。例如，所述植物、植物部分或植物生物质材料可以用于燃烧反应、气化、热解或多糖水解(酶法或化学法)。在某些实施方案中，所述植物、植物部分或植物生物质材料被用于糖化反应，例如，酶促糖化，以制备与野生型植物相比增加的量的可溶性糖。在某些实施方案中，所述植物、植物部分或植物生物质材料被用于与野生型植物相比增加生物质产量或简化木材工业(诸如造纸、制浆和建筑)的下游加工。在某些实施方案中，所述植物、植物部分或植物生物质材料被用于提高用于建筑目的的木材的质量。
在某些实施方案中，使用细胞壁(组成或含量)的修饰来增加秆/茎强度以减少谷类(小麦、大麦、玉米….)的倒伏和种子损失。
转化(例如生物质气化)的方法是本领域已知的。简而言之，在气化中，将植物或植物生物质材料(例如，叶和茎)粉碎成小颗粒，并与受控量的空气或氧和蒸汽一起输入气化器中。该反应的热和压力会断裂生物质的化学键，从而形成合成气，所述合成气随后被净化以除去杂质诸如硫、汞、微粒和痕量物质。然后可以将合成气转化成产品，诸如乙醇或其它生物燃料。
酶促糖化方法也是本领域已知的。简而言之，任选地用热水或稀酸预处理植物或植物生物质材料(例如，叶和茎)，随后使用纤维素和β-葡萄糖苷酶在缓冲液中的混合物进行酶促糖化，并将植物或植物生物质材料与所述酶混合物一起温育。温育后，可以如下容易地确定糖化反应的收率：使用标准的糖检测方法，例如本领域技术人员众所周知的二硝基水杨酸方法，测量释放的还原糖的量。根据本发明工程改造的植物会提供与野生型植物相比更高的糖收率。
实施例
提供下述实施例来例证、而不是限制要求保护的发明。
实施例1:重新工程改造植物中的次生细胞壁沉积
该研究合并了2个方案，用于克服细胞壁不顺从和给纤维细胞填充细胞壁聚合物且不改变植物发育。第一个方案允许减少除了导管以外的地方的木质素，而第二个方案会特异性地增加在木质组织中的细胞壁沉积。该组合方案策略使用合成生物学来精细调整木质素生物合成和建立新反馈回路从而重新工程改造次生细胞壁沉积的控制。
材料和方法
质粒的构建
从拟南芥cDNA扩增C4H(ref3)基因(AT2G30490)、F5H(At4g36220)和CADc基因(AT3G19450)的蛋白编码区，并用适当引物(参见表1)扩增在VND6基因(At5g62380)的翻译起始位点的5'上游的2756bp区域，作为基因组DNA的pVND6。
表1.用于质粒构建和基因分型的引物(SEQ ID NOS:328-339)
pVND6-F3-KpnI 5'-cccgggtaccTCCTTTACGATGTTGTTATGGGTTA-3'
pVND6-R3-SpeI 5'-cccgactagtGTGTGCGAGACTTTGGATTTGATCTTTTTAATTTTA-3'
FY100908-C4h-GW-F 5'-ggggacaagtttgtacaaaaaagcaggcttcATGGACCTCCTCTTGCTGGA-3'
FY100908-C4h-GW-R 5'-ggggaccactttgtacaagaaagctgggtcACAGTTCCTTGGTTTCATAACG-3'
DL-F5G3-At3g19450-GW 5'-ggggacaagtttgtacaaaaaagcaggcttcATGGGAAGTGTAGAAGCAGGAGAA-3'
DL-R5G3-At3g19450-GW 5'-ggggaccactttgtacaagaaagctgggtcGTTTGTAGTTGTTGCAGCCTCCTC-3'
FY081508-F5h-1-GW-F 5'-ggggacaagtttgtacaaaaaagcaggcttcATGGAGTCTT CTATATCACA A-3'
FY081508-F5h-1-GW-R 5'-ggggaccactttgtacaagaaagctgggtcAAGAGCACAGATGAGGCGCGT-3'
ref3-2F1 5'-TTC CGT ATC ATG TTC GAT AG-3'
ref3-2R1 5'-AAT GTC AAT TTC CCA AAA TC-3'
pcr-pVND6F1 5'-CAAATTGCCACATTGCAGAA-3'
pcr-REF3-R1 5'-CGACGAGATTACGGTGGTTGA-3'
将入口片段(Invitrogen)引入pCAMBIA1390中，并使用KpnI-SpeI/AvrII位点克隆VND6启动子，然后通过入口系统将C4H和CADc基因引入表达载体中，以获得最终的表达载体pCAMBIA1390-pVND6:C4H、pCAMBIA1390-pVND6:F5H和pCAMBIA1390-pVND6:CADc。
植物生长和转化
使拟南芥属植物在土壤中在22℃生长，每天8小时光照(短光照条件)持续4-5周，和每天16小时光照(短光照条件)持续4-5周。
通过电穿孔将表达载体pCAMBIA1390-pVND6:C4H、pCAMBIA1390-pVND6:F5H或pCAMBIA1390-pVND6:CADc引入根瘤土壤杆菌菌株GV3101中，并用于使用花浸法分别转染拟南芥属f5h、cadc/d纯合子ref3-2(c4h突变体)杂合子、f5h纯合子和cadc/d纯合子突变体植物(Clough和Bent,1998)。
拟南芥属植物的基因型分析
播种ref3-2杂合子突变体的种子，通过CTAB方法提取植物的基因组DNA，并用引物ref3-2F1和ref3-2R1(参见表1)通过PCR分析基因型。用HinfI消化PCR产物。预期的PCR产物是188bp和106bp片段(对于野生型植物)和294bp片段(对于ref3-2纯合子)。
用引物pcr-pVND6F1和pcr-REF3-R1通过PCR鉴别pVND6:C4H的转化体。转化体的PCR产物是238bp。使用DyNAzyme DNA聚合酶(Finnzymes,USA)进行上述的PCR反应。
RNA分离和cDNA合成
使用RNeasy Plant Mini Kit(Qiagen,Valencia,CA)，从在短光照条件下保持4周的拟南芥属植物的叶子分离出总RNA。使用Transcriptor High Fidelity cDNA SynthesisKit(Roche Applied Science,Indianapolis,IN)，合成cDNA。
显微术分析
为了研究茎细胞的木质素含量和解剖学，从突变体、野生型和转基因系的茎的基部制备横切面(当植物高度是30-35cm(对于健康植物)、15-20cm(对于突变体植物)时)。将成熟植物的茎基部包埋进7％琼脂糖中，然后使用振动切片机(Leica VT1000S)切片至100μm的厚度。将切片在水中固定，并在明视野下检查。还在紫外照明下观察木质化的细胞壁。木质素是紫外吸收剂，所以木质化的细胞壁在紫外照明下会发射蓝色自发荧光。将2％(w/v)的间苯三酚溶解在乙醇和浓HCl的2:1混合物中的溶液直接施加于茎切片，以检测所有木质素(Adler,1977)。还用卡尔科弗卢尔(对诸如纤维素等β-葡聚糖特异性的染料)将茎切片染色，以确定细胞的一般解剖学(Mori,1996)。将新鲜切片浸入0.5％卡尔科弗卢尔中保持5分钟，随后用水洗涤2次各5分钟，以除去任何多余的未结合的卡尔科弗卢尔。立即使用荧光显微镜(Leica DM4000B)观察切片。使用Leica DC500照相机记录图像。
醇不溶性的残余物(AIR)的制备
收集植物茎，干燥，并研磨成粉末，然后根据Goubet等人(2009)制备醇不溶性的残余物(AIR)。将研磨的茎粉末与1mL 95％乙醇一起混合，并在100℃温育30min。离心后，除去上清液，并用1mL 70％乙醇洗涤沉淀物2～3次，并彻底干燥。
木质素测量
通过乙酰基溴方法(Fukushima,2004)，分析了5mg AIR样品进行木质素测定。在具有螺旋盖的2mL埃彭道夫管中将AIR样品与200uL丙酮溴化物溶液(25％v/v的乙酰基溴在冰醋酸中的溶液)混合，在50℃在600rpm摇动2小时，然后用乙酸稀释至1mL的总体积。离心后，将100uL上清液转移至新试管，并分别与500uL乙酸、300uL 0.3M氢氧化钠和100uL盐酸羟胺混合，然后用乙酸稀释至2mL的总体积。将360uL该溶液转移至紫外特异性的96-孔平板(Greiner,Monroe,NC)，并在280nm读出吸光度。基于公开的消光系数(Fukushima,2004；Foster,2010)，计算乙酰基溴可溶性的木质素的百分比(％ABSL)。
糖化和DNS测定
用170uL水、稀碱(1％ NaOH，在30℃30min，在100℃30min)或稀酸(1.2％ H2SO4，在30℃30min，在120℃1小时)预处理5mg AIR样品。加入HCl或NaOH来中和最后的预处理样品，然后给所述样品加入8uL 5mg/mL四环素、25uL 1M柠檬酸盐缓冲液pH 6.2、2uL稀释的酶混合液(Novozyme酶NS50013(纤维素酶)和NS50010(β-葡萄糖苷酶)，分别在0.1M柠檬酸盐缓冲液pH 5.0中1:10和1:100稀释)，并用水稀释至500ul的终体积。将样品在50℃在850rpm摇动24小时。糖化后，通过DNS测定来分析糖量。使用0、0.125、0.25、0.5、0.75、1和2mg/mL的在柠檬酸盐缓冲液pH 5.0中的葡萄糖作为标准品。将DNS试剂加入样品和标准品中，在95℃温育10min，然后在540nm读出吸光度进行测定。
半纤维素组成分析
在120℃在1ml 2M TFA中将大约5mg AIR水解1h。通过在真空下干燥，除去TFA。随后如以前所述(Obro,2004；Christensen,2010)，使用PA20柱(Dionex,Sunnyvale,CA)通过水解的材料的HPAEC-PAD来确定单糖组成。单糖标准品包括L-Fuc、L-Rha、L-Ara、D-Gal、D-Glc、D-Xyl、D-GalA和D-GlcA，并得自Sigma。为了验证应答因子，在分析每批样品之前进行标准校准。
结果
导管特异性的启动子pVND6的表征
由于导管组织在向光合器官运输水和营养物中的重要性，良好的植物发育需要导管的完整性。VND-型转录因子已经被表征为导管形成的主要调节剂，这提示它们具有限于导管的表达谱(Kubo等人,2005)。为了将这些转录因子的时空表达与木质素生物合成相关联，使用启动子pVND6来补充CAD突变体(参见Sibout等人,2005)(图20中A)。木质部的红色消失和导管完整性的恢复是使用该启动子的验收准则。
为了对比启动子pVND6和启动子pC4H的强度，使用两种启动子来补充f5h突变体(Meyer等人,1998)。通过使用Maule染色作为读出来测量掺入木质素中的芥子醇单元的量，对比所述启动子的活性(图20中B)。在Maule染色后，与使用pC4H的系相比，在VND6启动子下表达F5H基因的系的茎横截面表现出远远更低的红色。这些结果指示，在pVND6:F5H系中芥子醇在木质素中的积累是由于与pC4H:F5H系相比更低的且更受限的F5H活性，该发现与上述的cadc/d互补相一致(图20中A)。
木质素生物合成的限制
木质素生物合成途径得到了充分表征，并且木质素生物合成途径的几个基因中的任一个的功能缺失都会导致有害的生长效应和不育。因此，控制这些基因之一的表达应当会提供控制木质醇单体产生的机会。我们选择了C4H基因(木质素生物合成途径中的一个早期基因)作为靶基因来控制产生木质醇单体的途径的通量。为了控制C4H的表达，我们使用了ref3-2突变体(Schilmiller等人,2009)，并且用含有pVND6:C4H基因构建体的二元载体转化了杂合子系(由于不育)。选择转化体，并关于ref3-2等位基因的纯合体型进行基因分型。令人感兴趣的是，携带pVND6:C4H片段的ref3-2纯合子，其被称作“EngSCW1g”(经工程改造的次生细胞壁第1代)，与同时培养的Col0野生型植物相比没有表现出生长差异。这些转化的植物能够产生大花结和高茎，并且是能育的(图16中A)。但是，由于花青苷仅积累在导管中，得自转化的植物的叶子是紫色，与此相比，野生型叶子在高光下变成完全紫色。该结果证实了pVND6启动子与pC4H相比的受限活性。
通过乙酰基溴方法进行的EngSCW1g植物的木质素含量分析表明，在衰老茎中的木质素含量接近在相同条件下同时培养的Col0茎植物的木质素含量的大约2/3。为了证实茎中的木质素分布，使用间苯三酚和Maule染色方法，分析了约15-20cm老茎的横截面。与在它的天然C4H启动子控制下表达C4H基因的野生型植物相比，经工程改造的系的横截面表现出维管束间纤维的木质素染色的减少。与纯合子ref3-2突变体相比，EngCW1g植物的木质部组织表现出强烈的间苯三酚染色且没有表现出导管塌缩，这类似于野生型植物(图15中B和图21)。
细胞壁沉积的增加
控制导管和纤维中的次生细胞壁沉积的转录网络已经得到了充分研究。次生细胞壁沉积由2个独立的网络控制，尽管这2个网络会导致下游次生壁生物合成基因的相同集合的活化来调节纤维素、半纤维素和木质素的合成。几个研究组已经证实，在拟南芥属中用组成活性的35S启动子过表达次生细胞壁转录因子会产生异位次生细胞壁和到处木质化，包括在伸长细胞和光合组织中，其结果是抑制植物生长(Zhong等人,2008；Mitsuda等人,2005；Goicoechea等人,2005)。令人感兴趣的是，尽管具有受限的发育，所述植物在纤维细胞中表现出增强的次生细胞壁厚度(Zhong等人,2008)，这提示，增加次生细胞壁转录因子的表达可以成为增加细胞壁沉积(并因此增加生物质密度)的一个途径。
因此，我们用IRX8启动子在EngCW1g植物中过表达了NST1cDNA。因为IRX8是在NST1转录因子的下游(即，由其控制)的基因(Mitsuda等人,2005；Zhong等人,2010)，该pIRX8:NST1构建体会建立正反馈回路，用于仅在次生细胞壁组织中过表达NST1cDNA。选择EngCW1g植物用于转化，因为VND6启动子不是NST1的下游靶标，并且因此在EngCW1g中在pVND6控制下的木质素生物合成将与NST1调节断开。制备的植物，其被称作“EngSCW2g”(经工程改造的次生细胞壁第2代)，与同时培养的Col0和EngSCW1g植物相比没有表现出生长差异。EngSCW2g植物能够产生大花结和高茎，并且是能育的(图17中A)。象EngSCW1g植物一样，由于花青苷仅积累在导管中，得自EngSCW2g系的叶子是紫色，与此相比，野生型叶子在高光下变成完全紫色。通过半定量PCR验证了NST1基因(天然的和cDNA)的表达的证实，并揭示，天然NST1在野生型、EngSCW1g和EngSCW2g系中以相同水平表达。但是，仅在EngSCW2g系中检测到新NST1拷贝的表达，从而导致NST1基因(天然的和cDNA)在茎中更高的总体表达水平(图22)。
为了证实NST1过表达对茎中细胞壁沉积的影响，使用间苯三酚染色法分析了老茎的茎横截面中的木质素分布。EngSCW2g系的横截面仍然表现出与野生型相比维管束间纤维的木质素染色的减少，而木质部组织表现出强烈的间苯三酚染色且没有表现出导管塌缩，这类似于野生型和EngSCW1g系(图15中B和17中B)。通过透射电子显微术(TEM)，对得自xxxcm老茎的基部的横截面分析了细胞壁增厚。在得自维管束间纤维的纤维细胞和木质部中观察到EngSCW2g系与野生型相比强烈的细胞壁增厚，但是在导管中没有观察到(图18和23)，这与NST转录因子的过表达相一致(Zhong等人,2008)。在野生型茎横截面中，观察到常见的4个独特层(S1、S2和S3和胞间层)，与此相比，在EngSCW2g系中，观察到具有不同强度的额外层，所述层几乎填满整个细胞空间。
为了生物能精细调节次生细胞壁沉积
用金标记的CBM对得自EngSCW2g植物的细胞壁横截面的分析揭示，所述额外细胞壁层含有纤维素，这提示，已经增加了纤维素的量。为了证实纤维素的增加，使用H2SO4对得自EngSCW2g的衰老茎进行了完全多糖水解(Suilter等人2008,Technical report NREL/TP-510-4218)。从茎细胞壁释放的葡萄糖和其它糖的量在野生型、EngSCW1g和EngSCW2g系之间是类似的。木糖和葡糖醛酸的量也增加，这提示，这些植物中的半纤维素沉积也增加。通过三氟乙酸(TFA)水解对得自EngSCW1g和EngSCW2g系的成熟茎进行的半纤维素组成分析没有表现出与同时培养的野生型植物相比的重大差异(图24)。
为了分析EngSCW2g系的糖化效率，对得自EngSCW2g系的5mg球磨茎进行2种不同的温和预处理：热水和稀碱，随后进行糖化动力学。在每种预处理后，在有纤维素酶混合液存在下，葡萄糖远远更快地从茎释放，并且当在120小时糖化之前分别进行碱和热水预处理时，EngSCW2g植物是对照植物的2-3倍(图19中A-B)。
还用EngSCW1g系观察到糖化改善；对于那些植物，在热水或稀碱预处理以后在有相同量的纤维素酶存在下水解的糖分别是热水或稀碱预处理后对照植物的2.3和1.5倍。NST1转录因子在EngSCW2g系中的过表达增加了细胞壁沉积，但是没有降低糖化效率，这可以解释为，因为该系与亲本EngSCW1g系相比增加的多糖含量，该系释放出更高的葡萄糖量。
被修饰成表达C4H的其它ref3-2突变体植物的分析
还使用启动子pREF4或pRFR1，将Ref3-2突变体植物工程改造成表达C4H。将该突变体植物修饰成含有pREF4:C4H或prFR1:C4H以表达C4H。分析了经工程改造的细胞壁植物系的植物生长和表型。图29显示了植物的照片。在用任一种构建体转化的突变体植物中恢复了生长。在图30中显示了植物的木质素分布。结果表明，在经工程改造的植物中，木质素在导管中产生，但是在纤维中减少，这导致与野生型植物相比总木质素减少了>35％，且没有影响植物生长。图31提供的数据表明了经工程改造的系的糖化效率。这些结果表明，木质素在纤维中的减少极大地提高了糖化效率。因此，这些结果证实，可以使用启动子pREF4和pRFR1来工程改造象“EngSCW1g”植物(用pVND6:C4H构建体补充的ref3-2)一样含有低木质素的植物，并用作次生细胞壁正反馈回路的遗传背景。
实施例2.在拟南芥属(双子叶植物)和短柄草属(单子叶植物)中经工程改造的正 反馈回路
图27解释了细胞壁沉积正反馈回路。细胞壁致密化是基于，建立人工正反馈回路来增强纤维特异性的转录因子的表达。通过表达在木聚糖或纤维素生物合成的下游诱导型启动子控制下的纤维特异性的转录因子(例如，NST1)的新拷贝来建立它。该方案与木聚糖和木质素工程改造策略相容。
图31中的A显示了被遗传修饰成含有pCesA4:NST1表达构建体的野生型拟南芥属(双子叶植物)和野生型拟南芥属的茎横截面的紫外图像。含有次生细胞壁纤维素启动子(pCesA4)和次生细胞壁转录因子(NST1)的正反馈回路的建立会增强纤维细胞中的次生细胞壁沉积。
图31中的B显示了被遗传修饰成含有pAtIRX8:AtNST1表达构建体的野生型短柄草属(单子叶植物)和野生型短柄草属的茎横截面的紫外图像。含有次生细胞壁纤维素启动子(pAtIRX8)和次生细胞壁转录因子(AtNST1)的正反馈回路的建立会增强短柄草属中的次生细胞壁沉积。
本实施例证实，该途径在单子叶植物和双子叶植物中都是保守的，并且可以建立正反馈回路来增强次生细胞壁沉积。
实施例3.工程改造木聚糖生物合成酶
从拟南芥属生物资源中心(Arabidopsis Biological Resource Center)得到拟南芥属突变体irx7-1(At2g28110,salk_120296)、irx8-1(At5g54690,salk_008642)、irx9-1(At2g37090,salk_058238)、irx9-2(salk_057033C)、parvus(At1g19300,CS16279)。将野生型IRX7、IRX8、IRX9和PARVUS基因克隆进Gateway入口克隆中，并如上面关于木质素生物合成基因所述与pVND6或pVND7启动子一起重组进Gateway目标载体中。
通过电穿孔将表达载体pCAMBIA1390-pVND6:IRX7、pCAMBIA1390-pVND7:IRX7、pCAMBIA1390-pVND6:IRX8、pCAMBIA1390-pVND7:IRX8、pCAMBIA1390-pVND6:IRX9、pCAMBIA1390-pVND7:IRX9、pCAMBIA1390-pVND6:PARVUS、pCAMBIA1390-pVND7:PARVUS引入根瘤土壤杆菌菌株GV3101中。使用花浸法(Clough和Bent,1998)，使用表达IRX7、IRX8、IRX9和PARVUS的构建体转化拟南芥属杂合子突变体植物(分别是irx7-1、irx8-1、irx9-1和parvus)。还使用表达IRX9的构建体转化irx9-2的纯合突变体。
将转化的irx7、irx8、parvus、irx9-1和irx9-2植物的种子播种在补充了潮霉素的生长培养基上。回收湿霉素抗性的植物，并转移至土壤。所述植物表现出健康的生长表型，这不同于未转化的纯合的突变体，后者的生长明显受到影响。
选择转化的irx7、irx8、irx9-2、parvus和irx9-1突变体。通过PCR表征回收的转化的突变体以确保它们关于原始突变的纯合表型，并确保pVND6或pVND7驱动的转基因的存在。将所述植物的生长与野生型和纯合突变体的生长进行对比，并通过花序茎的糖组成分析来确定它们的木聚糖含量。通过乙酰基溴方法来确定木质素。通过免疫荧光显微术使用LM10抗体确定木聚糖沉积的集中，并通过显微术和在紫外照明和间苯三酚染色下自发荧光的确定来确定木质素的沉积。如上所述确定糖化。
图33提供的数据证实，在IRX7、IRX8或IRX9基因中的突变体表现出强烈的生长减少。构建体(其中突变基因的野生型形式由pVND6或pVND7启动子驱动)对所述突变体的转化恢复了生长。用pVND6:IRX9和pVND7:IRX7得到了类似的结果。
图34提供的数据表明了通过用pVND7:IRX7构建体转化irx7突变体制备的4个单个转化体的后代的生长。通过测量花结直径来定量生长。2个植物系与野生型(Col0)相同地生长，而1个植物系比野生型植物稍微更好地生长，并且对于1种植物，生长仅部分地恢复。
图35提供的数据表明了通过用pVND7:IRX9构建体转化irx9突变体制备的2个单个转化体的后代的生长。通过测量花结直径来定量生长。转化的植物系与野生型(Col0)相同地生长。用pVND6:IRX9转化的植物得到了类似的结果。
图36提供的数据表明了通过用pVND7:IRX7构建体转化irx7突变体制备的4个单个转化体所制备的细胞壁的非纤维质单糖组成分析。所有转化体仍然表现出原始irx7突变体的低木聚糖含量，但是恢复了生长。
图37提供的数据表明了通过用pVND6:IRX8构建体转化irx8突变体制备的4个单个转化体的后代所制备的细胞壁的非纤维质单糖组成分析。所有转化体仍然表现出原始irx8突变体的低木聚糖含量，但是恢复了生长。
图38提供的数据表明了通过用pVND7:IRX9构建体转化irx9突变体制备的4个单个转化体和含有pVND6:IRX9构建体的单个转化体的后代所制备的茎细胞壁的非纤维质单糖组成分析。所有转化体仍然表现出原始irx9突变体的低木聚糖含量，但是恢复了生长。
图39提供的数据表明了通过用pVND6:IRX9构建体转化irx9突变体制备的2个单个转化体和通过用pVND7:IRX9构建体转化irx9突变体制备的3个单个转化体的后代所制备的细胞壁的糖化分析。所有转化体表现出与原始irx9突变体类似的糖化改善，但是恢复了生长。
实施例4.蜡-APFL在表皮细胞中的产生和在物种之间的保守
蜡类是高能的(highly energetic)，且含有大量具有潜在燃料应用的长链烷烃和脂肪酸。因此，使用蜡-APFL来制备能够在茎的非必需组织(诸如髓心和纤维)中产生和积累大量蜡类的植物，会提供产生具有高能量密度并且还有效地利用水的生物能作物的新机会。
图28解释了蜡沉积的人工正反馈回路。
本实施例采用拟南芥属作为模型植物来开发蜡-APFL，以增加表皮细胞中的蜡生物合成和积累。设计了8个DNA构建体来在表皮细胞(其产生一些蜡)中产生蜡AFPL。使用pAtCER1或pAtWBC11作为启动子来制备这些构建体，以从拟南芥属表达AtSHN1(NP_172988)和分别从水稻、短柄草属和卷柏属表达选择的同系物OsSHN1(NP_001046226),BdSHN1(XP_003563662)或SmSHN1(XP_002969836)。使用土壤杆菌转化，将所有构建体单个地转移进野生型拟南芥属中。对于每种蜡-APFL，回收几个转基因植物。
象在许多植物种中一样，在拟南芥属中，蜡生物合成主要发生在得自叶和茎的表皮细胞中。几项研究还已经报道，使用组成型或化学诱导型启动子过表达SHN基因的植物会产生叶或/和茎表面的光泽表型，这归因于蜡沉积或/和组成的修饰(McNevin等人1993；Broun等人2004；Kannangara等人2007；Shi等人.2011)。用不同构建体转化的拟南芥属植物的视觉分析表现出增加的叶光亮(图40)。
对纯合系进行了其它分析，包括叶和茎表皮蜡的组成分析。植物发育、叶表皮光亮的其它评估、叶绿素浸取测定、蜡积累和组成分析、基因表达分析、以及对干旱胁迫和水损耗的生物影响，是用于表征植物中的蜡-APFL的主要标准。叶绿素浸取测定是鉴别角质层的乙醇渗透性的修饰的一般测定，并通过监测在有乙醇存在下对完整叶的叶绿素提取来进行(Aharoni等人,Plant Cell 2004出处同上；Seo等人,Plant Cell 2011,出处同上)。在通过将完整叶或茎短时间浸入氯仿(其含有一些正-三十烷作为标准品)中进行提取以后，分析了上角质层的蜡积累和组成。通过TLC平板，使用在90:7.5:1溶剂系统中的己烷:乙醚:乙酸，预分析了所述提取物的一般组成，并用99:1的N,O双(三甲基甲硅烷基)三氟乙酰胺):三甲基氯硅烷衍生化，用于GC/MS分析(Aharoni等人.Plant Cell,2004,出处同上；Kannangara等人,Plant Cell,2007,出处同上)。为了评价增强的蜡沉积对植物水利用效率的影响，通过监测重量减轻对分离的叶进行了水损耗测定。最后，通过5-6周龄植物在7-15天脱水期和随后的1周供水恢复期以后的植物存活计数，研究了蜡沉积修饰对植物干旱胁迫耐受性的影响。
讨论
修饰木质素含量始终是作物或树的一项挑战，因为减少得越严重，生物质产量受到的影响越多。该减少也经常与导管组织的完整性丧失有关，所述导管组织负责水和营养物从根向地上器官的运输和分布。木质素是植物细胞壁多糖的有效酶促水解的主要抑制因子之一。因此，我们的策略聚焦于减少除了导管以外(为了维持导管完整性)的大部分组织中的木质素，并聚焦于木质素生物合成与关键次生细胞壁转录因子开关的断开，以便操纵所述转录因子的表达而不影响木质素沉积。
我们的重新工程改造次生细胞壁生物合成的策略证实，我们可以在木质组织中减少木质素含量和增加细胞壁增厚，且不改变植物生长。将控制木质素生物合成中的必需步骤的基因的启动子替换为另一个具有更受限的时空表达谱的启动子，会提供比单独的沉默方案更好的木质素沉积控制。该精细调节会避免每个组织中的木质素沉积的减少，并允许使它保持在诸如导管等必需组织中，与此相比，沉默方案会影响每个组织，并因此限制了这样的策略的功效。pVND6启动子用于控制C4H的活性的用途，允许木质素生物合成与控制纤维细胞中的次生细胞壁沉积的一般转录因子网络部分地断开，并首次允许在没有过度木质化情况下增加多糖沉积。为了仅在具有自我诱导的木质组织中增加次生细胞壁沉积，我们使用pIRX8启动子制备了人工正反馈回路，以表达主要转录因子NST1的第二拷贝。该启动子在产生次生细胞壁的组织中具有特异性活性，并且在纤维细胞中已经处于NST1转录因子控制下。因此，这样的嵌合基因允许通过自我诱导来过表达NST1，还增加在多糖生物合成中涉及的下游靶基因的表达。另外，使用NST1的下游启动子来表达它自身的新拷贝，可能已经增加了NST1转录因子的时间依赖性表达，因此增加纤维细胞中的次生细胞壁沉积的时间，从而增加细胞壁厚度。
据我们所知，在植物中仅建立了一个人工负反馈回路来调节发育过程，并且它对应于衰老的延迟(Gan和Amasino,Science 1995)。该策略对应于使用早期衰老诱导型启动子(pSAG12)在衰老过程开始时表达编码异戊烯基转移酶的IPT基因，以便产生在该阶段特异性的细胞分裂素。已知该激素会抑制衰老过程和更长久地保持植物光合活性(Gan和Amasino,Science 1995)。由于衰老过程的调节机制和基因网络在物种之间的保守，和尤其是激素细胞分裂素对衰老过程的延迟，将该合成构建体转移进不同的作物(草和双子叶植物)中，并且可以由于植物的寿命的增加而提高生物质产量(McCabe等人,2001；Lin等人,Acta Botanica Sinica 2002,44:1333-1338；Robson等人,2004；Li等人,2004；Swartzberg等人,2006；Calderini等人,2007；Li等人,Plant Physiology 2010；和Chen等人,Molecular Breeding 2001)。
次生细胞壁生物合成与保守调节网络属于相同类别，因为该生物学过程在维管植物内相当保守(Zhong等人,2010)。例如，在次生细胞壁生物合成中涉及的转录网络和基因相当保守。该网络的保守允许我们利用植物拟南芥属模型，从而实现该方案的快速试验和稳健性。因为增加的多糖含量具有从生物能至造纸工业的多种应用(包括饲料作物)，该策略的可转移性需要是通用的。本文所述的方案应当是相容的，且可快速地从模型物种转移至生物能作物(双子叶植物和单子叶植物)。以前已经证实，在物种之间过表达次生细胞壁转录因子会产生类似的表型和功能，这提示，启动子调节元件也是相当保守的。参见，例如，Shen等人,2009 Bioenerg.Res 2:217-232；Zhong等人,2010 Plant Physiol 152:1044-1055；Goicoechea等人2005Plant J 43:553-567；Franke等人,2000,Plant J.22:223-234。因此，应当不需要靶作物的基因组序列，并且可以使用得自其它物种(诸如拟南芥属或作物相关的物种)的盒启动子(例如，pIRX5)和转录因子(例如，NST1)来转化靶植物。
不同于酵母、大肠杆菌、立碗藓属和少数其它物种，仍然必须开发通过体内重组在植物中实现的启动子替换；因此，为了操纵组织特异性的木质素沉积，需要突变体。由于突变的有害效应，在作物中难以得到在木质素生物合成途径的必需基因中的天然功能缺失突变体。另外，尚未在植物中开发出组织/细胞特异性的基因表达抑制。因此，经常使用一般的沉默策略来修饰基因表达，以便降低作物中的酶活性，这至少需要在靶向的生物合成途径中涉及的基因的EST序列。与木质素生物合成途径有关的一个担心是，在基因抑制水平、植物健康和所需表型之间的折中经常是相矛盾的。例如，通过抑制木质醇单体生物合成中涉及的基因来改善糖化，极其常见地会影响导管完整性，从而影响水和营养物运输，并因此影响植物生长。为了将所述技术转移至作物，可以使用遗传密码的简并性(密码子选择的灵活性)来制备沉默的抗性的木质素基因，其与沉默构建体一起将用得自拟南芥属或靶作物的相关物种的导管特异性启动子表达，以减少或消除对应的天然基因的表达。例如，在杨树中用导管特异性的启动子(诸如VND6)表达不同的4CL编码序列，会恢复4CL反义系的生长和生物质产量(Kitin等人,2010；Voelker等人,2010)，并保持良好的糖化效率。可替换地，可以开发绕过缺陷性酶促步骤的策略。例如，可以在表达C3H RNAi的杨树中用导管特异性的启动子表达得自卷柏属的SmF5H基因，以恢复导管的完整性和正常的植物生长(Coleman等人,2008a,2008b)。最近在拟南芥属中证实，该SmF5H基因能够恢复HCT和C3H缺陷型突变体的生长(Li等人,2010Plant Cell 22:1620-1632)和分别缺乏产生莽草酸对香豆酰酯和将莽草酸对香豆酰酯偏位羟基化(它们是木质素生物合成中的必需步骤)的能力的木质素突变体的生长(Weng等人2010)。与该SmF5H策略类似地，通过使用将酪氨酸转化成对羟基香豆酸的酪氨酸氨裂合酶(TAL)基因，可以绕过两个将苯丙氨酸转化成对香豆酸的酶促步骤。
总之，我们已经证实，2个方案(1个用于增加细胞生物质密度，1个用于将木质素生物合成限制在含有导管的必需组织中)是相容的，并允许制备具有大量非反抗性细胞壁的健康植物，从而允许在没有严重预处理的情况下有效地酶促转化成可发酵的糖。这些方案开启了作物优化的新领域，并且将有利于木质纤维素生物燃料、造纸和饲料工业。
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Lee C,Teng Q,Huang W,Zhong R,Ye ZH(2009)Down-regulation of PoGT47Cexpression in poplar results in a reduced glucuronoxylan content and anincreased wood digestibility by cellulase.Plant Cell Physiol 50:1075-1089
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应当理解，本文所述的实施例和实施方案仅用于例证目的，并且提示本领域技术人员可以以此为依据进行各种变型或改变，且它们包括在本申请的精神和范围和待批权利要求范围内。在本文中引用的所有出版物、专利、登录号和专利申请特此通过引用整体并入用于所有目的。
在蜡/角质生物合成中涉及的示例性基因：包括登录号和同义的基因命名。
AtCER1:At1g02205:醛脱羰酶
AtCER2:VC2:At4g24510:BAHD-型酰基-转移酶
AtCER3:WAX2:At5g57800:甾醇去饱和酶
AtCER4:FAR3:At4g33790:脂肪酰基辅酶A还原酶
AtCER5:WBC12:ABCG12:At1g51500:ABC转运蛋白
AtCER6:CUT1:KCS6:At1g68530:极长链脂肪酸缩合酶
AtCER10:ECR:At3g55360:烯酰辅酶A还原酶
AtWSD1:At5g37300:蜡酯合酶
AtMAH1:CYP96A15:At1g57750:中链烷烃水解酶
AtWBC11:ABCG11:DSO:COF1:At1g17840:ABC转运蛋白
AtKCS1:At1g01120:极长链脂肪酸缩合酶
AtKCS2:DAISY:At1g04220:极长链脂肪酸缩合酶
AtFATB:At1g08510:酰基载体
AtLACS1:At2g47240:长链酰基辅酶A合酶
AtLACS2:At1g49430:长链酰基辅酶A合酶
AtCYP86A4:At1g01600:细胞色素P450依赖性的脂肪酸羟化酶
AtCYP86A7:At1g63710:细胞色素P450依赖性的脂肪酸羟化酶
AtLCR:CYP86A5:At2g45970:细胞色素P450依赖性的脂肪酸羟化酶
AtKCS10:FDH:At2g26250:极长链脂肪酸缩合酶
AtCER60:KCS5:At1g25450:极长链脂肪酸缩合酶
序列表
<110> 加利福尼亚大学董事会(THE REGENTS OF THE UNIVERSITY OF CALIFORNIA)
<120> 植物中经空间修饰的基因表达
<130> 77429-829402
<140> WO PCT/US12/23182
<141> 2012-01-30
<150> US 61/437,569
<151> 2011-01-28
<160> 339
<170> SIPOSequenceListing 1.0
<210> 1
<211> 2178
<212> DNA
<213> 拟南芥()
<220>
<223> 苯丙氨酸氨裂合酶 (PAL1), At2g37040
<400> 1
atggagatta acggggcaca caagagcaac ggaggaggag tggacgctat gttatgcggc 60
ggagacatca agacaaagaa catggtgatc aacgcggagg atcctctcaa ctggggagct 120
gcagcggagc aaatgaaagg tagccatttg gatgaagtga agagaatggt tgctgagttt 180
aggaagccag ttgtgaatct tggtggtgag actctgacca ttggacaagt ggctgcgatc 240
tcaactattg gtaacagtgt gaaggtggag ctatcggaga cagctagagc cggtgtgaat 300
gctagtagtg attgggttat ggagagtatg aacaaaggca ctgatagtta tggtgttact 360
actggttttg gtgctacttc tcatcggaga accaaaaacg gtgtcgcact tcagaaggaa 420
cttattagat tccttaacgc cggaatattc ggaagcacga aagaaacaag ccacacattg 480
ccacactccg ccacaagagc cgccatgctt gtacgaatca acactctcct ccaaggattt 540
tccggtatcc gatttgagat tctcgaagca attaccagtt tcctcaacaa caacatcact 600
ccatctctcc ccctccgtgg tacaatcacc gcctccggag atctcgttcc tctctcctac 660
atcgccggac ttctcaccgg tcgtcccaat tccaaagcta ctggtcccaa cggtgaagct 720
ttaacagcag aggaagcttt caaattagca ggaatcagct ccggattctt tgatctccag 780
cctaaggaag gtctcgcgct agtcaatggc acggcggttg gatctggaat ggcgtcaatg 840
gtgttattcg aaacgaatgt tctctctgtt ttggctgaga ttttgtcggc ggttttcgca 900
gaggtgatga gtggtaagcc tgagttcacc gatcatctca ctcacagact taaacatcat 960
cccggtcaaa tcgaagcggc ggcgataatg gagcatatcc tcgacggaag ctcgtacatg 1020
aaattagctc agaagcttca cgagatggat ccgttacaga aacctaaaca agatcgttac 1080
gctcttcgta cttctcctca atggttaggt cctcaaatcg aagtgatccg ttacgcaacg 1140
aaatcgatcg agcgtgagat taactccgtc aacgataatc cgttgatcga tgtttcgagg 1200
aacaaggcga ttcacggtgg taacttccaa ggaacaccaa tcggagtttc aatggataac 1260
acgagattgg cgatagcagc gattggtaaa ctcatgtttg ctcaattctc agagcttgtg 1320
aatgatttct acaacaatgg tttaccctcg aatctaaccg cttcgaggaa tccaagtttg 1380
gattatggat tcaagggagc tgagattgca atggcttctt attgttcaga gcttcaatac 1440
ttagctaatc ctgtgactag ccatgttcaa tcagcagagc aacataacca agatgtcaac 1500
tctttgggac taatctcgtc tcgcaaaact tctgaagctg ttgatattct caagcttatg 1560
tcaacaacgt tcctcgttgc gatttgtcaa gctgtggatt tgagacattt ggaggagaat 1620
ttgagacaga ctgtgaagaa cactgtctct caagtggcga agaaagttct tactactgga 1680
gtcaatggtg agcttcatcc ttctcgcttc tgcgaaaagg atttactcaa agttgtagac 1740
cgtgaacaag tctacacata cgcggatgat ccttgtagcg caacgtaccc gttgattcag 1800
aagctgagac aagttattgt tgaccatgct ttgatcaatg gtgagagtga gaagaatgca 1860
gtgacttcaa tcttccataa gattggagct ttcgaggagg agcttaaggc agtgctaccg 1920
aaagaagtgg aagcagcaag agcagcctac gataacggaa catcggctat cccgaacagg 1980
atcaaggaat gtaggtcgta tccattgtat agattcgtga gggaagagct tggaacagag 2040
cttttgaccg gagagaaagt gacgtcgcct ggagaagagt tcgacaaggt tttcacggcg 2100
atttgtgaag gtaaaatcat tgatccgatg atggaatgtc tcaacgagtg gaacggagct 2160
cccattccaa tatgttaa 2178
<210> 2
<211> 725
<212> PRT
<213> 拟南芥()
<220>
<223> 苯丙氨酸氨裂合酶 (PAL1, AtPAL1)
<400> 2
Met Glu Ile Asn Gly Ala His Lys Ser Asn Gly Gly Gly Val Asp Ala
1 5 10 15
Met Leu Cys Gly Gly Asp Ile Lys Thr Lys Asn Met Val Ile Asn Ala
20 25 30
Glu Asp Pro Leu Asn Trp Gly Ala Ala Ala Glu Gln Met Lys Gly Ser
35 40 45
His Leu Asp Glu Val Lys Arg Met Val Ala Glu Phe Arg Lys Pro Val
50 55 60
Val Asn Leu Gly Gly Glu Thr Leu Thr Ile Gly Gln Val Ala Ala Ile
65 70 75 80
Ser Thr Ile Gly Asn Ser Val Lys Val Glu Leu Ser Glu Thr Ala Arg
85 90 95
Ala Gly Val Asn Ala Ser Ser Asp Trp Val Met Glu Ser Met Asn Lys
100 105 110
Gly Thr Asp Ser Tyr Gly Val Thr Thr Gly Phe Gly Ala Thr Ser His
115 120 125
Arg Arg Thr Lys Asn Gly Val Ala Leu Gln Lys Glu Leu Ile Arg Phe
130 135 140
Leu Asn Ala Gly Ile Phe Gly Ser Thr Lys Glu Thr Ser His Thr Leu
145 150 155 160
Pro His Ser Ala Thr Arg Ala Ala Met Leu Val Arg Ile Asn Thr Leu
165 170 175
Leu Gln Gly Phe Ser Gly Ile Arg Phe Glu Ile Leu Glu Ala Ile Thr
180 185 190
Ser Phe Leu Asn Asn Asn Ile Thr Pro Ser Leu Pro Leu Arg Gly Thr
195 200 205
Ile Thr Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr Ile Ala Gly Leu
210 215 220
Leu Thr Gly Arg Pro Asn Ser Lys Ala Thr Gly Pro Asn Gly Glu Ala
225 230 235 240
Leu Thr Ala Glu Glu Ala Phe Lys Leu Ala Gly Ile Ser Ser Gly Phe
245 250 255
Phe Asp Leu Gln Pro Lys Glu Gly Leu Ala Leu Val Asn Gly Thr Ala
260 265 270
Val Gly Ser Gly Met Ala Ser Met Val Leu Phe Glu Thr Asn Val Leu
275 280 285
Ser Val Leu Ala Glu Ile Leu Ser Ala Val Phe Ala Glu Val Met Ser
290 295 300
Gly Lys Pro Glu Phe Thr Asp His Leu Thr His Arg Leu Lys His His
305 310 315 320
Pro Gly Gln Ile Glu Ala Ala Ala Ile Met Glu His Ile Leu Asp Gly
325 330 335
Ser Ser Tyr Met Lys Leu Ala Gln Lys Leu His Glu Met Asp Pro Leu
340 345 350
Gln Lys Pro Lys Gln Asp Arg Tyr Ala Leu Arg Thr Ser Pro Gln Trp
355 360 365
Leu Gly Pro Gln Ile Glu Val Ile Arg Tyr Ala Thr Lys Ser Ile Glu
370 375 380
Arg Glu Ile Asn Ser Val Asn Asp Asn Pro Leu Ile Asp Val Ser Arg
385 390 395 400
Asn Lys Ala Ile His Gly Gly Asn Phe Gln Gly Thr Pro Ile Gly Val
405 410 415
Ser Met Asp Asn Thr Arg Leu Ala Ile Ala Ala Ile Gly Lys Leu Met
420 425 430
Phe Ala Gln Phe Ser Glu Leu Val Asn Asp Phe Tyr Asn Asn Gly Leu
435 440 445
Pro Ser Asn Leu Thr Ala Ser Arg Asn Pro Ser Leu Asp Tyr Gly Phe
450 455 460
Lys Gly Ala Glu Ile Ala Met Ala Ser Tyr Cys Ser Glu Leu Gln Tyr
465 470 475 480
Leu Ala Asn Pro Val Thr Ser His Val Gln Ser Ala Glu Gln His Asn
485 490 495
Gln Asp Val Asn Ser Leu Gly Leu Ile Ser Ser Arg Lys Thr Ser Glu
500 505 510
Ala Val Asp Ile Leu Lys Leu Met Ser Thr Thr Phe Leu Val Ala Ile
515 520 525
Cys Gln Ala Val Asp Leu Arg His Leu Glu Glu Asn Leu Arg Gln Thr
530 535 540
Val Lys Asn Thr Val Ser Gln Val Ala Lys Lys Val Leu Thr Thr Gly
545 550 555 560
Val Asn Gly Glu Leu His Pro Ser Arg Phe Cys Glu Lys Asp Leu Leu
565 570 575
Lys Val Val Asp Arg Glu Gln Val Tyr Thr Tyr Ala Asp Asp Pro Cys
580 585 590
Ser Ala Thr Tyr Pro Leu Ile Gln Lys Leu Arg Gln Val Ile Val Asp
595 600 605
His Ala Leu Ile Asn Gly Glu Ser Glu Lys Asn Ala Val Thr Ser Ile
610 615 620
Phe His Lys Ile Gly Ala Phe Glu Glu Glu Leu Lys Ala Val Leu Pro
625 630 635 640
Lys Glu Val Glu Ala Ala Arg Ala Ala Tyr Asp Asn Gly Thr Ser Ala
645 650 655
Ile Pro Asn Arg Ile Lys Glu Cys Arg Ser Tyr Pro Leu Tyr Arg Phe
660 665 670
Val Arg Glu Glu Leu Gly Thr Glu Leu Leu Thr Gly Glu Lys Val Thr
675 680 685
Ser Pro Gly Glu Glu Phe Asp Lys Val Phe Thr Ala Ile Cys Glu Gly
690 695 700
Lys Ile Ile Asp Pro Met Met Glu Cys Leu Asn Glu Trp Asn Gly Ala
705 710 715 720
Pro Ile Pro Ile Cys
725
<210> 3
<211> 1518
<212> DNA
<213> 拟南芥()
<220>
<223> 肉桂酸4-羟化酶 (C4H), At2g30490
<400> 3
atggacctcc tcttgctgga gaagtcttta atcgccgtct tcgtggcggt gattctcgcc 60
acggtgattt caaagctccg cggcaagaaa ttgaagctac ctccaggtcc tataccaatt 120
ccgatcttcg gaaactggct tcaagtcgga gatgatctca accaccgtaa tctcgtcgat 180
tacgctaaga aattcggcga tctcttcctc ctccgtatgg gtcagcgaaa cctagtcgtc 240
gtctcctcac cggatctaac aaaggaagtg ctcctcactc aaggcgttga gtttggatcc 300
agaacgagaa acgtcgtgtt cgacattttc accgggaaag gtcaagatat ggtgttcact 360
gtttacggcg agcattggag gaagatgaga agaatcatga cggttccttt cttcaccaac 420
aaagttgttc aacagaatcg tgaaggttgg gagtttgaag cagctagtgt tgttgaagat 480
gttaagaaga atccagattc tgctacgaaa ggaatcgtgt tgaggaaacg tttgcaattg 540
atgatgtata acaatatgtt ccgtatcatg ttcgatagaa gatttgagag tgaggatgat 600
cctcttttcc ttaggcttaa ggctttgaat ggtgagagaa gtcgattagc tcagagcttt 660
gagtataact atggagattt cattcctatc cttagaccat tcctcagagg ctatttgaag 720
atttgtcaag atgtgaaaga tcgaagaatc gctcttttca agaagtactt tgttgatgag 780
aggaagcaaa ttgcgagttc taagcctaca ggtagtgaag gattgaaatg tgccattgat 840
cacatccttg aagctgagca gaagggagaa atcaacgagg acaatgttct ttacatcgtc 900
gagaacatca atgtcgccgc gattgagaca acattgtggt ctatcgagtg gggaattgca 960
gagctagtga accatcctga aatccagagt aagctaagga acgaactcga cacagttctt 1020
ggaccgggtg tgcaagtcac cgagcctgat cttcacaaac ttccatacct tcaagctgtg 1080
gttaaggaga ctcttcgtct gagaatggcg attcctctcc tcgtgcctca catgaacctc 1140
catgatgcga agctcgctgg ctacgatatc ccagcagaaa gcaaaatcct tgttaatgct 1200
tggtggctag caaacaaccc caacagctgg aagaagcctg aagagtttag accagagagg 1260
ttctttgaag aagaatcgca cgtggaagct aacggtaatg acttcaggta tgtgccattt 1320
ggtgttggac gtcgaagctg tcccgggatt atattggcat tgcctatttt ggggatcacc 1380
attggtagga tggtccagaa cttcgagctt cttcctcctc caggacagtc taaagtggat 1440
actagtgaga aaggtggaca attcagcttg cacatcctta accactccat aatcgttatg 1500
aaaccaagga actgttaa 1518
<210> 4
<211> 505
<212> PRT
<213> 拟南芥()
<220>
<223> 肉桂酸4-羟化酶 (C4H, AtC4H)
<400> 4
Met Asp Leu Leu Leu Leu Glu Lys Ser Leu Ile Ala Val Phe Val Ala
1 5 10 15
Val Ile Leu Ala Thr Val Ile Ser Lys Leu Arg Gly Lys Lys Leu Lys
20 25 30
Leu Pro Pro Gly Pro Ile Pro Ile Pro Ile Phe Gly Asn Trp Leu Gln
35 40 45
Val Gly Asp Asp Leu Asn His Arg Asn Leu Val Asp Tyr Ala Lys Lys
50 55 60
Phe Gly Asp Leu Phe Leu Leu Arg Met Gly Gln Arg Asn Leu Val Val
65 70 75 80
Val Ser Ser Pro Asp Leu Thr Lys Glu Val Leu Leu Thr Gln Gly Val
85 90 95
Glu Phe Gly Ser Arg Thr Arg Asn Val Val Phe Asp Ile Phe Thr Gly
100 105 110
Lys Gly Gln Asp Met Val Phe Thr Val Tyr Gly Glu His Trp Arg Lys
115 120 125
Met Arg Arg Ile Met Thr Val Pro Phe Phe Thr Asn Lys Val Val Gln
130 135 140
Gln Asn Arg Glu Gly Trp Glu Phe Glu Ala Ala Ser Val Val Glu Asp
145 150 155 160
Val Lys Lys Asn Pro Asp Ser Ala Thr Lys Gly Ile Val Leu Arg Lys
165 170 175
Arg Leu Gln Leu Met Met Tyr Asn Asn Met Phe Arg Ile Met Phe Asp
180 185 190
Arg Arg Phe Glu Ser Glu Asp Asp Pro Leu Phe Leu Arg Leu Lys Ala
195 200 205
Leu Asn Gly Glu Arg Ser Arg Leu Ala Gln Ser Phe Glu Tyr Asn Tyr
210 215 220
Gly Asp Phe Ile Pro Ile Leu Arg Pro Phe Leu Arg Gly Tyr Leu Lys
225 230 235 240
Ile Cys Gln Asp Val Lys Asp Arg Arg Ile Ala Leu Phe Lys Lys Tyr
245 250 255
Phe Val Asp Glu Arg Lys Gln Ile Ala Ser Ser Lys Pro Thr Gly Ser
260 265 270
Glu Gly Leu Lys Cys Ala Ile Asp His Ile Leu Glu Ala Glu Gln Lys
275 280 285
Gly Glu Ile Asn Glu Asp Asn Val Leu Tyr Ile Val Glu Asn Ile Asn
290 295 300
Val Ala Ala Ile Glu Thr Thr Leu Trp Ser Ile Glu Trp Gly Ile Ala
305 310 315 320
Glu Leu Val Asn His Pro Glu Ile Gln Ser Lys Leu Arg Asn Glu Leu
325 330 335
Asp Thr Val Leu Gly Pro Gly Val Gln Val Thr Glu Pro Asp Leu His
340 345 350
Lys Leu Pro Tyr Leu Gln Ala Val Val Lys Glu Thr Leu Arg Leu Arg
355 360 365
Met Ala Ile Pro Leu Leu Val Pro His Met Asn Leu His Asp Ala Lys
370 375 380
Leu Ala Gly Tyr Asp Ile Pro Ala Glu Ser Lys Ile Leu Val Asn Ala
385 390 395 400
Trp Trp Leu Ala Asn Asn Pro Asn Ser Trp Lys Lys Pro Glu Glu Phe
405 410 415
Arg Pro Glu Arg Phe Phe Glu Glu Glu Ser His Val Glu Ala Asn Gly
420 425 430
Asn Asp Phe Arg Tyr Val Pro Phe Gly Val Gly Arg Arg Ser Cys Pro
435 440 445
Gly Ile Ile Leu Ala Leu Pro Ile Leu Gly Ile Thr Ile Gly Arg Met
450 455 460
Val Gln Asn Phe Glu Leu Leu Pro Pro Pro Gly Gln Ser Lys Val Asp
465 470 475 480
Thr Ser Glu Lys Gly Gly Gln Phe Ser Leu His Ile Leu Asn His Ser
485 490 495
Ile Ile Val Met Lys Pro Arg Asn Cys
500 505
<210> 5
<211> 1671
<212> DNA
<213> 拟南芥()
<220>
<223> 4-香豆酸-CoA连接酶 (4CL2), At3g21240
<400> 5
atgacgacac aagatgtgat agtcaatgat cagaatgatc agaaacagtg tagtaatgac 60
gtcattttcc gatcgagatt gcctgatata tacatcccta accacctccc actccacgac 120
tacatcttcg aaaatatctc agagttcgcc gctaagccat gcttgatcaa cggtcccacc 180
ggcgaagtat acacctacgc cgatgtccac gtaacatctc ggaaactcgc cgccggtctt 240
cataacctcg gcgtgaagca acacgacgtt gtaatgatcc tcctcccgaa ctctcctgaa 300
gtagtcctca ctttccttgc cgcctccttc atcggcgcaa tcaccacctc cgcgaacccg 360
ttcttcactc cggcggagat ttctaaacaa gccaaagcct ccgcggcgaa actcatcgtc 420
actcaatccc gttacgtcga taaaatcaag aacctccaaa acgacggcgt tttgatcgtc 480
accaccgact ccgacgccat ccccgaaaac tgcctccgtt tctccgagtt aactcagtcc 540
gaagaaccac gagtggactc aataccggag aagatttcgc cagaagacgt cgtggcgctt 600
cctttctcat ccggcacgac gggtctcccc aaaggagtga tgctaacaca caaaggtcta 660
gtcacgagcg tggcgcagca agtcgacggc gagaatccga atctttactt caacagagac 720
gacgtgatcc tctgtgtctt gcctatgttc catatatacg ctctcaactc catcatgctc 780
tgtagtctca gagttggtgc cacgatcttg ataatgccta agttcgaaat cactctcttg 840
ttagagcaga tacaaaggtg taaagtcacg gtggctatgg tcgtgccacc gatcgtttta 900
gctatcgcga agtcgccgga gacggagaag tatgatctga gctcggttag gatggttaag 960
tctggagcag ctcctcttgg taaggagctt gaagatgcta ttagtgctaa gtttcctaac 1020
gccaagcttg gtcagggcta tgggatgaca gaagcaggtc cggtgctagc aatgtcgtta 1080
gggtttgcta aagagccgtt tccagtgaag tcaggagcat gtggtacggt ggtgaggaac 1140
gccgagatga agatacttga tccagacaca ggagattctt tgcctaggaa caaacccggc 1200
gaaatatgca tccgtggcaa ccaaatcatg aaaggctatc tcaatgaccc cttggccacg 1260
gcatcgacga tcgataaaga tggttggctt cacactggag acgtcggatt tatcgatgat 1320
gacgacgagc ttttcattgt ggatagattg aaagaactca tcaagtacaa aggatttcaa 1380
gtggctccag ctgagctaga gtctctcctc ataggtcatc cagaaatcaa tgatgttgct 1440
gtcgtcgcca tgaaggaaga agatgctggt gaggttcctg ttgcgtttgt ggtgagatcg 1500
aaagattcaa atatatccga agatgaaatc aagcaattcg tgtcaaaaca ggttgtgttt 1560
tataagagaa tcaacaaagt gttcttcact gactctattc ctaaagctcc atcagggaag 1620
atattgagga aggatctaag agcaagacta gcaaatggat taatgaacta g 1671
<210> 6
<211> 556
<212> PRT
<213> 拟南芥()
<220>
<223> 4-香豆酸-CoA连接酶 (4CL2, At4CL2)
<400> 6
Met Thr Thr Gln Asp Val Ile Val Asn Asp Gln Asn Asp Gln Lys Gln
1 5 10 15
Cys Ser Asn Asp Val Ile Phe Arg Ser Arg Leu Pro Asp Ile Tyr Ile
20 25 30
Pro Asn His Leu Pro Leu His Asp Tyr Ile Phe Glu Asn Ile Ser Glu
35 40 45
Phe Ala Ala Lys Pro Cys Leu Ile Asn Gly Pro Thr Gly Glu Val Tyr
50 55 60
Thr Tyr Ala Asp Val His Val Thr Ser Arg Lys Leu Ala Ala Gly Leu
65 70 75 80
His Asn Leu Gly Val Lys Gln His Asp Val Val Met Ile Leu Leu Pro
85 90 95
Asn Ser Pro Glu Val Val Leu Thr Phe Leu Ala Ala Ser Phe Ile Gly
100 105 110
Ala Ile Thr Thr Ser Ala Asn Pro Phe Phe Thr Pro Ala Glu Ile Ser
115 120 125
Lys Gln Ala Lys Ala Ser Ala Ala Lys Leu Ile Val Thr Gln Ser Arg
130 135 140
Tyr Val Asp Lys Ile Lys Asn Leu Gln Asn Asp Gly Val Leu Ile Val
145 150 155 160
Thr Thr Asp Ser Asp Ala Ile Pro Glu Asn Cys Leu Arg Phe Ser Glu
165 170 175
Leu Thr Gln Ser Glu Glu Pro Arg Val Asp Ser Ile Pro Glu Lys Ile
180 185 190
Ser Pro Glu Asp Val Val Ala Leu Pro Phe Ser Ser Gly Thr Thr Gly
195 200 205
Leu Pro Lys Gly Val Met Leu Thr His Lys Gly Leu Val Thr Ser Val
210 215 220
Ala Gln Gln Val Asp Gly Glu Asn Pro Asn Leu Tyr Phe Asn Arg Asp
225 230 235 240
Asp Val Ile Leu Cys Val Leu Pro Met Phe His Ile Tyr Ala Leu Asn
245 250 255
Ser Ile Met Leu Cys Ser Leu Arg Val Gly Ala Thr Ile Leu Ile Met
260 265 270
Pro Lys Phe Glu Ile Thr Leu Leu Leu Glu Gln Ile Gln Arg Cys Lys
275 280 285
Val Thr Val Ala Met Val Val Pro Pro Ile Val Leu Ala Ile Ala Lys
290 295 300
Ser Pro Glu Thr Glu Lys Tyr Asp Leu Ser Ser Val Arg Met Val Lys
305 310 315 320
Ser Gly Ala Ala Pro Leu Gly Lys Glu Leu Glu Asp Ala Ile Ser Ala
325 330 335
Lys Phe Pro Asn Ala Lys Leu Gly Gln Gly Tyr Gly Met Thr Glu Ala
340 345 350
Gly Pro Val Leu Ala Met Ser Leu Gly Phe Ala Lys Glu Pro Phe Pro
355 360 365
Val Lys Ser Gly Ala Cys Gly Thr Val Val Arg Asn Ala Glu Met Lys
370 375 380
Ile Leu Asp Pro Asp Thr Gly Asp Ser Leu Pro Arg Asn Lys Pro Gly
385 390 395 400
Glu Ile Cys Ile Arg Gly Asn Gln Ile Met Lys Gly Tyr Leu Asn Asp
405 410 415
Pro Leu Ala Thr Ala Ser Thr Ile Asp Lys Asp Gly Trp Leu His Thr
420 425 430
Gly Asp Val Gly Phe Ile Asp Asp Asp Asp Glu Leu Phe Ile Val Asp
435 440 445
Arg Leu Lys Glu Leu Ile Lys Tyr Lys Gly Phe Gln Val Ala Pro Ala
450 455 460
Glu Leu Glu Ser Leu Leu Ile Gly His Pro Glu Ile Asn Asp Val Ala
465 470 475 480
Val Val Ala Met Lys Glu Glu Asp Ala Gly Glu Val Pro Val Ala Phe
485 490 495
Val Val Arg Ser Lys Asp Ser Asn Ile Ser Glu Asp Glu Ile Lys Gln
500 505 510
Phe Val Ser Lys Gln Val Val Phe Tyr Lys Arg Ile Asn Lys Val Phe
515 520 525
Phe Thr Asp Ser Ile Pro Lys Ala Pro Ser Gly Lys Ile Leu Arg Lys
530 535 540
Asp Leu Arg Ala Arg Leu Ala Asn Gly Leu Met Asn
545 550 555
<210> 7
<211> 1302
<212> DNA
<213> 拟南芥()
<220>
<223> 羟基肉桂酰辅酶A:莽草酸羟基肉桂酰基转移酶 (HCT), At5g48930
<400> 7
atgaaaatta acatcagaga ttccaccatg gtccggcctg ccaccgagac accaatcact 60
aatctttgga actccaacgt cgaccttgtc atccccagat tccatacccc tagtgtctac 120
ttctacagac ccaccggcgc ttccaatttc tttgaccctc aggtcatgaa ggaagctctt 180
tccaaagccc ttgtcccttt ttaccctatg gctggtcgct tgaagagaga cgatgatggt 240
cgtattgaga tcgattgtaa cggtgctggt gttctcttcg ttgtggctga tactccttct 300
gttatcgatg attttggtga ttttgctcct acccttaatc tccgtcagct tattcccgaa 360
gttgatcact ccgctggcat tcactctttc ccgcttctcg ttttgcaggt gactttcttt 420
aaatgtgggg gagcttcact tggggttggg atgcaacatc acgcggcaga tggtttctct 480
ggtcttcatt ttatcaacac atggtctgat atggctcgtg gtcttgacct aaccattcca 540
cctttcattg atcgaacact cctccgagct agggacccgc cacagcctgc ttttcatcat 600
gttgaatatc agcctgcacc aagtatgaag atacctcttg atccgtctaa atcaggacct 660
gagaatacca ctgtctctat attcaaatta acacgagacc agcttgttgc tcttaaggcg 720
aaatccaagg aggatgggaa cactgtcagc tacagctcat acgagatgtt ggcagggcat 780
gtgtggagat cagtgggaaa ggcgcgaggg cttccaaacg accaagagac gaaactgtac 840
attgcaactg atggaaggtc tagactacgt ccgcagctgc ctcctggtta ctttgggaat 900
gtgatattca ctgcaacacc attggctgtt gcaggggatt tgttatctaa gccaacatgg 960
tatgctgcag gacagattca tgatttcttg gttcgtatgg atgataacta tctgaggtca 1020
gctcttgact acctggagat gcagcctgat ctgtcagccc ttgtccgcgg tgcacatacc 1080
tacaagtgcc caaatttggg aatcacaagc tgggttagat tacctattta tgatgcagac 1140
tttggttggg gtcgtcctat ctttatggga cctggtggaa ttccatacga gggtttgtct 1200
tttgtgctac caagtcctac taatgatggc agcttatccg ttgccattgc cctccaatct 1260
gaacacatga aactgtttga gaagtttttg tttgagatat ga 1302
<210> 8
<211> 433
<212> PRT
<213> 拟南芥()
<220>
<223> 羟基肉桂酰辅酶A:莽草酸羟基肉桂酰基转移酶 (HCT, AtHCT)
<400> 8
Met Lys Ile Asn Ile Arg Asp Ser Thr Met Val Arg Pro Ala Thr Glu
1 5 10 15
Thr Pro Ile Thr Asn Leu Trp Asn Ser Asn Val Asp Leu Val Ile Pro
20 25 30
Arg Phe His Thr Pro Ser Val Tyr Phe Tyr Arg Pro Thr Gly Ala Ser
35 40 45
Asn Phe Phe Asp Pro Gln Val Met Lys Glu Ala Leu Ser Lys Ala Leu
50 55 60
Val Pro Phe Tyr Pro Met Ala Gly Arg Leu Lys Arg Asp Asp Asp Gly
65 70 75 80
Arg Ile Glu Ile Asp Cys Asn Gly Ala Gly Val Leu Phe Val Val Ala
85 90 95
Asp Thr Pro Ser Val Ile Asp Asp Phe Gly Asp Phe Ala Pro Thr Leu
100 105 110
Asn Leu Arg Gln Leu Ile Pro Glu Val Asp His Ser Ala Gly Ile His
115 120 125
Ser Phe Pro Leu Leu Val Leu Gln Val Thr Phe Phe Lys Cys Gly Gly
130 135 140
Ala Ser Leu Gly Val Gly Met Gln His His Ala Ala Asp Gly Phe Ser
145 150 155 160
Gly Leu His Phe Ile Asn Thr Trp Ser Asp Met Ala Arg Gly Leu Asp
165 170 175
Leu Thr Ile Pro Pro Phe Ile Asp Arg Thr Leu Leu Arg Ala Arg Asp
180 185 190
Pro Pro Gln Pro Ala Phe His His Val Glu Tyr Gln Pro Ala Pro Ser
195 200 205
Met Lys Ile Pro Leu Asp Pro Ser Lys Ser Gly Pro Glu Asn Thr Thr
210 215 220
Val Ser Ile Phe Lys Leu Thr Arg Asp Gln Leu Val Ala Leu Lys Ala
225 230 235 240
Lys Ser Lys Glu Asp Gly Asn Thr Val Ser Tyr Ser Ser Tyr Glu Met
245 250 255
Leu Ala Gly His Val Trp Arg Ser Val Gly Lys Ala Arg Gly Leu Pro
260 265 270
Asn Asp Gln Glu Thr Lys Leu Tyr Ile Ala Thr Asp Gly Arg Ser Arg
275 280 285
Leu Arg Pro Gln Leu Pro Pro Gly Tyr Phe Gly Asn Val Ile Phe Thr
290 295 300
Ala Thr Pro Leu Ala Val Ala Gly Asp Leu Leu Ser Lys Pro Thr Trp
305 310 315 320
Tyr Ala Ala Gly Gln Ile His Asp Phe Leu Val Arg Met Asp Asp Asn
325 330 335
Tyr Leu Arg Ser Ala Leu Asp Tyr Leu Glu Met Gln Pro Asp Leu Ser
340 345 350
Ala Leu Val Arg Gly Ala His Thr Tyr Lys Cys Pro Asn Leu Gly Ile
355 360 365
Thr Ser Trp Val Arg Leu Pro Ile Tyr Asp Ala Asp Phe Gly Trp Gly
370 375 380
Arg Pro Ile Phe Met Gly Pro Gly Gly Ile Pro Tyr Glu Gly Leu Ser
385 390 395 400
Phe Val Leu Pro Ser Pro Thr Asn Asp Gly Ser Leu Ser Val Ala Ile
405 410 415
Ala Leu Gln Ser Glu His Met Lys Leu Phe Glu Lys Phe Leu Phe Glu
420 425 430
Ile
<210> 9
<211> 780
<212> DNA
<213> 拟南芥()
<220>
<223> 豆酰基莽草酸3-羟化酶 (C3H), At4g34050
<400> 9
atggcgacga caacaacaga agcaacgaag acatcatcga ccaatggaga agatcagaag 60
cagtctcaga atcttcgaca tcaagaagtt ggtcacaaga gtctcttaca gagcgatgat 120
ctctaccagt atatactgga gacaagtgtg tatcctagag aaccagaatc aatgaaggaa 180
ctcagggaag tgacagcaaa acatccatgg aacataatga ccacatcagc tgatgaagga 240
cagttcttaa acatgcttat caagctcgtt aacgccaaga acacaatgga gatcggagtt 300
tacactggct actctcttct cgccaccgct cttgctctcc ctgaagacgg caaaattctg 360
gctatggatg tcaacagaga gaattacgaa ttgggtttac cgatcattga gaaagccggc 420
gttgctcaca agatcgactt cagggaaggc cctgctcttc ccgttcttga tgaaatcgtt 480
gctgacgaga agaaccatgg aacatatgac tttatattcg ttgatgctga caaagacaac 540
tacatcaact accacaagcg tttgatcgat cttgtgaaaa ttggaggagt gattggctac 600
gacaacactc tgtggaatgg ttctgtcgtg gctcctcctg atgcaccaat gaggaagtac 660
gttcgttact acagagactt tgttcttgag cttaacaagg ctcttgctgc tgaccctcgg 720
atcgagatct gtatgctccc tgttggtgat ggaatcacta tctgccgtcg gatcagttga 780
<210> 10
<211> 508
<212> PRT
<213> 拟南芥()
<220>
<223> 豆酰基莽草酸3-羟化酶 (C3H, AtC3H)
<400> 10
Met Ser Trp Phe Leu Ile Ala Val Ala Thr Ile Ala Ala Val Val Ser
1 5 10 15
Tyr Lys Leu Ile Gln Arg Leu Arg Tyr Lys Phe Pro Pro Gly Pro Ser
20 25 30
Pro Lys Pro Ile Val Gly Asn Leu Tyr Asp Ile Lys Pro Val Arg Phe
35 40 45
Arg Cys Tyr Tyr Glu Trp Ala Gln Ser Tyr Gly Pro Ile Ile Ser Val
50 55 60
Trp Ile Gly Ser Ile Leu Asn Val Val Val Ser Ser Ala Glu Leu Ala
65 70 75 80
Lys Glu Val Leu Lys Glu His Asp Gln Lys Leu Ala Asp Arg His Arg
85 90 95
Asn Arg Ser Thr Glu Ala Phe Ser Arg Asn Gly Gln Asp Leu Ile Trp
100 105 110
Ala Asp Tyr Gly Pro His Tyr Val Lys Val Arg Lys Val Cys Thr Leu
115 120 125
Glu Leu Phe Thr Pro Lys Arg Leu Glu Ser Leu Arg Pro Ile Arg Glu
130 135 140
Asp Glu Val Thr Ala Met Val Glu Ser Val Phe Arg Asp Cys Asn Leu
145 150 155 160
Pro Glu Asn Arg Ala Lys Gly Leu Gln Leu Arg Lys Tyr Leu Gly Ala
165 170 175
Val Ala Phe Asn Asn Ile Thr Arg Leu Ala Phe Gly Lys Arg Phe Met
180 185 190
Asn Ala Glu Gly Val Val Asp Glu Gln Gly Leu Glu Phe Lys Ala Ile
195 200 205
Val Ser Asn Gly Leu Lys Leu Gly Ala Ser Leu Ser Ile Ala Glu His
210 215 220
Ile Pro Trp Leu Arg Trp Met Phe Pro Ala Asp Glu Lys Ala Phe Ala
225 230 235 240
Glu His Gly Ala Arg Arg Asp Arg Leu Thr Arg Ala Ile Met Glu Glu
245 250 255
His Thr Leu Ala Arg Gln Lys Ser Ser Gly Ala Lys Gln His Phe Val
260 265 270
Asp Ala Leu Leu Thr Leu Lys Asp Gln Tyr Asp Leu Ser Glu Asp Thr
275 280 285
Ile Ile Gly Leu Leu Trp Asp Met Ile Thr Ala Gly Met Asp Thr Thr
290 295 300
Ala Ile Thr Ala Glu Trp Ala Met Ala Glu Met Ile Lys Asn Pro Arg
305 310 315 320
Val Gln Gln Lys Val Gln Glu Glu Phe Asp Arg Val Val Gly Leu Asp
325 330 335
Arg Ile Leu Thr Glu Ala Asp Phe Ser Arg Leu Pro Tyr Leu Gln Cys
340 345 350
Val Val Lys Glu Ser Phe Arg Leu His Pro Pro Thr Pro Leu Met Leu
355 360 365
Pro His Arg Ser Asn Ala Asp Val Lys Ile Gly Gly Tyr Asp Ile Pro
370 375 380
Lys Gly Ser Asn Val His Val Asn Val Trp Ala Val Ala Arg Asp Pro
385 390 395 400
Ala Val Trp Lys Asn Pro Phe Glu Phe Arg Pro Glu Arg Phe Leu Glu
405 410 415
Glu Asp Val Asp Met Lys Gly His Asp Phe Arg Leu Leu Pro Phe Gly
420 425 430
Ala Gly Arg Arg Val Cys Pro Gly Ala Gln Leu Gly Ile Asn Leu Val
435 440 445
Thr Ser Met Met Ser His Leu Leu His His Phe Val Trp Thr Pro Pro
450 455 460
Gln Gly Thr Lys Pro Glu Glu Ile Asp Met Ser Glu Asn Pro Gly Leu
465 470 475 480
Val Thr Tyr Met Arg Thr Pro Val Gln Ala Val Ala Thr Pro Arg Leu
485 490 495
Pro Ser Asp Leu Tyr Lys Arg Val Pro Tyr Asp Met
500 505
<210> 11
<211> 1035
<212> DNA
<213> 拟南芥()
<220>
<223> 肉桂酰基辅酶A还原酶1 (CCR1), At1g15950
<400> 11
atgccagtcg acgtagcctc accggccgga aaaaccgtct gcgtcaccgg agctggtgga 60
tacatcgctt cttggattgt taagatactt ctcgagagag gttacacagt caaaggaacc 120
gtacggaatc cagatgatcc gaagaacaca catttgagag aactagaagg aggaaaggag 180
agactgattc tgtgcaaagc agatcttcag gactacgagg ctcttaaggc ggcgattgat 240
ggttgcgacg gcgtctttca cacggcttct cctgtcaccg acgatccgga acaaatggtg 300
gagccggccg tgaatggagc caagtttgta attaatgctg cggctgaggc caaggtcaag 360
cgcgtggtca tcacctcctc cattggtgcc gtctacatgg acccgaaccg tgaccctgag 420
gctgtcgttg acgaaagttg ttggagtgat cttgacttct gcaaaaacac caagaattgg 480
tattgttacg gcaagatggt ggcggaacaa gcggcgtggg agacagcaaa ggagaaaggt 540
gttgacttgg tggtgttgaa tccggtgctg gttcttggac cgccgttaca gccgacgatc 600
aacgccagtc tttaccacgt cctcaaatat ctaaccggct cggctaagac ttatgctaat 660
ttgactcaag cttatgtgga tgttcgcgat gtcgcgctgg ctcatgttct ggtctatgag 720
gcaccctcgg cctccggacg ttatctccta gccgagagtg ctcgccaccg cggggaagtt 780
gttgagattc tggctaagct attcccggag tatcctcttc cgaccaagtg caaggacgag 840
aagaacccta gagccaagcc atacaaattc actaaccaga agattaagga cttaggctta 900
gagttcactt ccaccaagca aagcctctac gacacagtca agagcttaca agagaaaggc 960
catcttgctc ctcctcctcc tcctccttca gcatcgcaag aatccgtgga aaatggcatt 1020
aagatcgggt cttga 1035
<210> 12
<211> 344
<212> PRT
<213> 拟南芥()
<220>
<223> 肉桂酰基辅酶A还原酶1 (CCR1, AtCCR1)
<400> 12
Met Pro Val Asp Val Ala Ser Pro Ala Gly Lys Thr Val Cys Val Thr
1 5 10 15
Gly Ala Gly Gly Tyr Ile Ala Ser Trp Ile Val Lys Ile Leu Leu Glu
20 25 30
Arg Gly Tyr Thr Val Lys Gly Thr Val Arg Asn Pro Asp Asp Pro Lys
35 40 45
Asn Thr His Leu Arg Glu Leu Glu Gly Gly Lys Glu Arg Leu Ile Leu
50 55 60
Cys Lys Ala Asp Leu Gln Asp Tyr Glu Ala Leu Lys Ala Ala Ile Asp
65 70 75 80
Gly Cys Asp Gly Val Phe His Thr Ala Ser Pro Val Thr Asp Asp Pro
85 90 95
Glu Gln Met Val Glu Pro Ala Val Asn Gly Ala Lys Phe Val Ile Asn
100 105 110
Ala Ala Ala Glu Ala Lys Val Lys Arg Val Val Ile Thr Ser Ser Ile
115 120 125
Gly Ala Val Tyr Met Asp Pro Asn Arg Asp Pro Glu Ala Val Val Asp
130 135 140
Glu Ser Cys Trp Ser Asp Leu Asp Phe Cys Lys Asn Thr Lys Asn Trp
145 150 155 160
Tyr Cys Tyr Gly Lys Met Val Ala Glu Gln Ala Ala Trp Glu Thr Ala
165 170 175
Lys Glu Lys Gly Val Asp Leu Val Val Leu Asn Pro Val Leu Val Leu
180 185 190
Gly Pro Pro Leu Gln Pro Thr Ile Asn Ala Ser Leu Tyr His Val Leu
195 200 205
Lys Tyr Leu Thr Gly Ser Ala Lys Thr Tyr Ala Asn Leu Thr Gln Ala
210 215 220
Tyr Val Asp Val Arg Asp Val Ala Leu Ala His Val Leu Val Tyr Glu
225 230 235 240
Ala Pro Ser Ala Ser Gly Arg Tyr Leu Leu Ala Glu Ser Ala Arg His
245 250 255
Arg Gly Glu Val Val Glu Ile Leu Ala Lys Leu Phe Pro Glu Tyr Pro
260 265 270
Leu Pro Thr Lys Cys Lys Asp Glu Lys Asn Pro Arg Ala Lys Pro Tyr
275 280 285
Lys Phe Thr Asn Gln Lys Ile Lys Asp Leu Gly Leu Glu Phe Thr Ser
290 295 300
Thr Lys Gln Ser Leu Tyr Asp Thr Val Lys Ser Leu Gln Glu Lys Gly
305 310 315 320
His Leu Ala Pro Pro Pro Pro Pro Pro Ser Ala Ser Gln Glu Ser Val
325 330 335
Glu Asn Gly Ile Lys Ile Gly Ser
340
<210> 13
<211> 1098
<212> DNA
<213> 拟南芥()
<220>
<223> 转录因子NAC次生壁增厚促进因子1 (NST1), ANAC043
<400> 13
atgatgtcaa aatctatgag catatcagtg aacggacaat ctcaagtgcc tcctgggttt 60
aggtttcatc cgaccgagga agagctgttg cagtattatc tccggaagaa agttaatagc 120
atcgagatcg atcttgatgt cattcgcgac gttgatctca acaagctcga gccttgggac 180
attcaagaga tgtgtaaaat aggaacaacg ccacaaaacg actggtattt ctttagccac 240
aaggacaaaa aatatccgac gggaacgaga actaacagag ccactgcggc tggattttgg 300
aaagcaactg gccgcgacaa gatcatatat agcaatggcc gtagaattgg gatgagaaag 360
actcttgttt tctacaaagg ccgagctcct cacggccaaa aatctgattg gatcatgcat 420
gaatatagac tcgatgacaa cattatttcc cccgaggatg tcaccgttca tgaggtcgtg 480
agtattatag gggaagcatc acaagacgaa ggatgggtgg tgtgtcgtat tttcaagaag 540
aagaatcttc acaaaaccct aaacagtccc gtcggaggag cttccctgag cggcggcgga 600
gatacgccga agacgacatc atctcagatc ttcaacgagg atactctcga ccaatttctt 660
gaacttatgg ggagatcttg taaagaagag ctaaatcttg accctttcat gaaactccca 720
aacctcgaaa gccctaacag tcaggcaatc aacaactgcc acgtaagctc tcccgacact 780
aatcataata tccacgtcag caacgtggtc gacactagct ttgttactag ctgggcggct 840
ttagaccgcc tcgtggcctc gcagcttaac ggacccacat catattcaat tacagccgtc 900
aatgagagcc acgtgggcca tgatcatctc gctttgcctt ccgtccgatc tccgtacccc 960
agcctaaacc ggtccgcttc gtaccacgcc ggtttaacac aggaatatac accggagatg 1020
gagctatgga atacgacgac gtcgtctcta tcgtcatcgc ctggcccatt ttgtcacgtg 1080
tcgaatggta gtggataa 1098
<210> 14
<211> 365
<212> PRT
<213> 拟南芥()
<220>
<223> 转录因子NAC次生壁增厚促进因子1 (NST1, AtNST1), ANAC043
<400> 14
Met Met Ser Lys Ser Met Ser Ile Ser Val Asn Gly Gln Ser Gln Val
1 5 10 15
Pro Pro Gly Phe Arg Phe His Pro Thr Glu Glu Glu Leu Leu Gln Tyr
20 25 30
Tyr Leu Arg Lys Lys Val Asn Ser Ile Glu Ile Asp Leu Asp Val Ile
35 40 45
Arg Asp Val Asp Leu Asn Lys Leu Glu Pro Trp Asp Ile Gln Glu Met
50 55 60
Cys Lys Ile Gly Thr Thr Pro Gln Asn Asp Trp Tyr Phe Phe Ser His
65 70 75 80
Lys Asp Lys Lys Tyr Pro Thr Gly Thr Arg Thr Asn Arg Ala Thr Ala
85 90 95
Ala Gly Phe Trp Lys Ala Thr Gly Arg Asp Lys Ile Ile Tyr Ser Asn
100 105 110
Gly Arg Arg Ile Gly Met Arg Lys Thr Leu Val Phe Tyr Lys Gly Arg
115 120 125
Ala Pro His Gly Gln Lys Ser Asp Trp Ile Met His Glu Tyr Arg Leu
130 135 140
Asp Asp Asn Ile Ile Ser Pro Glu Asp Val Thr Val His Glu Val Val
145 150 155 160
Ser Ile Ile Gly Glu Ala Ser Gln Asp Glu Gly Trp Val Val Cys Arg
165 170 175
Ile Phe Lys Lys Lys Asn Leu His Lys Thr Leu Asn Ser Pro Val Gly
180 185 190
Gly Ala Ser Leu Ser Gly Gly Gly Asp Thr Pro Lys Thr Thr Ser Ser
195 200 205
Gln Ile Phe Asn Glu Asp Thr Leu Asp Gln Phe Leu Glu Leu Met Gly
210 215 220
Arg Ser Cys Lys Glu Glu Leu Asn Leu Asp Pro Phe Met Lys Leu Pro
225 230 235 240
Asn Leu Glu Ser Pro Asn Ser Gln Ala Ile Asn Asn Cys His Val Ser
245 250 255
Ser Pro Asp Thr Asn His Asn Ile His Val Ser Asn Val Val Asp Thr
260 265 270
Ser Phe Val Thr Ser Trp Ala Ala Leu Asp Arg Leu Val Ala Ser Gln
275 280 285
Leu Asn Gly Pro Thr Ser Tyr Ser Ile Thr Ala Val Asn Glu Ser His
290 295 300
Val Gly His Asp His Leu Ala Leu Pro Ser Val Arg Ser Pro Tyr Pro
305 310 315 320
Ser Leu Asn Arg Ser Ala Ser Tyr His Ala Gly Leu Thr Gln Glu Tyr
325 330 335
Thr Pro Glu Met Glu Leu Trp Asn Thr Thr Thr Ser Ser Leu Ser Ser
340 345 350
Ser Pro Gly Pro Phe Cys His Val Ser Asn Gly Ser Gly
355 360 365
<210> 15
<211> 1005
<212> DNA
<213> 拟南芥()
<220>
<223> 转录因子NAC次生壁增厚促进因子2 (NST2), ANAC066, At3g61910
<400> 15
atgaacatat cagtaaacgg acagtcacaa gtacctcctg gctttaggtt tcacccaacc 60
gaggaagagc tcttgaagta ttacctccgc aagaaaatct ctaacatcaa gatcgatctc 120
gatgttattc ctgacattga tctcaacaag ctcgagcctt gggatattca agagatgtgt 180
aagattggaa cgacgccgca aaacgattgg tacttttata gccataagga caagaagtat 240
cccaccggga ctagaaccaa cagagccacc acggtcggat tttggaaagc gacgggacgt 300
gacaagacca tatataccaa tggtgataga atcgggatgc gaaagacgct tgtcttctac 360
aaaggtcgag cccctcatgg tcagaaatcc gattggatca tgcacgaata tagactcgac 420
gagagtgtat taatctcctc gtgtggcgat catgacgtca acgtagaaac gtgtgatgtc 480
ataggaagtg acgaaggatg ggtggtgtgt cgtgttttca agaaaaataa cctttgcaaa 540
aacatgatta gtagtagccc ggcgagttcg gtgaaaacgc cgtcgttcaa tgaggagact 600
atcgagcaac ttctcgaagt tatggggcaa tcttgtaaag gagagatagt tttagaccct 660
ttcttaaaac tccctaacct cgaatgccat aacaacacca ccatcacgag ttatcagtgg 720
ttaatcgacg accaagtcaa caactgccac gtcagcaaag ttatggatcc cagcttcatc 780
actagctggg ccgctttgga tcggctcgtt gcctcacagt taaatgggcc caactcgtat 840
tcaataccag ccgttaatga gacttcacaa tcaccgtatc atggactgaa ccggtccggt 900
tgtaataccg gtttaacacc agattactat ataccggaga ttgatttatg gaacgaggca 960
gatttcgcga gaacgacatg ccacttgttg aacggtagtg gataa 1005
<210> 16
<211> 334
<212> PRT
<213> 拟南芥()
<220>
<223> 转录因子NAC次生壁增厚促进因子2 (NST2), ANAC066
<400> 16
Met Asn Ile Ser Val Asn Gly Gln Ser Gln Val Pro Pro Gly Phe Arg
1 5 10 15
Phe His Pro Thr Glu Glu Glu Leu Leu Lys Tyr Tyr Leu Arg Lys Lys
20 25 30
Ile Ser Asn Ile Lys Ile Asp Leu Asp Val Ile Pro Asp Ile Asp Leu
35 40 45
Asn Lys Leu Glu Pro Trp Asp Ile Gln Glu Met Cys Lys Ile Gly Thr
50 55 60
Thr Pro Gln Asn Asp Trp Tyr Phe Tyr Ser His Lys Asp Lys Lys Tyr
65 70 75 80
Pro Thr Gly Thr Arg Thr Asn Arg Ala Thr Thr Val Gly Phe Trp Lys
85 90 95
Ala Thr Gly Arg Asp Lys Thr Ile Tyr Thr Asn Gly Asp Arg Ile Gly
100 105 110
Met Arg Lys Thr Leu Val Phe Tyr Lys Gly Arg Ala Pro His Gly Gln
115 120 125
Lys Ser Asp Trp Ile Met His Glu Tyr Arg Leu Asp Glu Ser Val Leu
130 135 140
Ile Ser Ser Cys Gly Asp His Asp Val Asn Val Glu Thr Cys Asp Val
145 150 155 160
Ile Gly Ser Asp Glu Gly Trp Val Val Cys Arg Val Phe Lys Lys Asn
165 170 175
Asn Leu Cys Lys Asn Met Ile Ser Ser Ser Pro Ala Ser Ser Val Lys
180 185 190
Thr Pro Ser Phe Asn Glu Glu Thr Ile Glu Gln Leu Leu Glu Val Met
195 200 205
Gly Gln Ser Cys Lys Gly Glu Ile Val Leu Asp Pro Phe Leu Lys Leu
210 215 220
Pro Asn Leu Glu Cys His Asn Asn Thr Thr Ile Thr Ser Tyr Gln Trp
225 230 235 240
Leu Ile Asp Asp Gln Val Asn Asn Cys His Val Ser Lys Val Met Asp
245 250 255
Pro Ser Phe Ile Thr Ser Trp Ala Ala Leu Asp Arg Leu Val Ala Ser
260 265 270
Gln Leu Asn Gly Pro Asn Ser Tyr Ser Ile Pro Ala Val Asn Glu Thr
275 280 285
Ser Gln Ser Pro Tyr His Gly Leu Asn Arg Ser Gly Cys Asn Thr Gly
290 295 300
Leu Thr Pro Asp Tyr Tyr Ile Pro Glu Ile Asp Leu Trp Asn Glu Ala
305 310 315 320
Asp Phe Ala Arg Thr Thr Cys His Leu Leu Asn Gly Ser Gly
325 330
<210> 17
<211> 1077
<212> DNA
<213> 拟南芥()
<220>
<223> 转录因子NAC次生壁增厚促进因子3 (NST3), SND1/ANAC012, At1g32770
<400> 17
atggctgata ataaggtcaa tctttcgatt aatggacaat caaaagtgcc tccaggtttc 60
agattccatc ccaccgaaga agaacttctc cattactatc tccgtaagaa agttaactct 120
caaaagatcg atcttgatgt cattcgtgaa gttgatctaa acaagcttga gccttgggat 180
attcaagagg aatgtagaat cggttcaacg ccacaaaacg actggtactt cttcagccac 240
aaggacaaga agtatccaac cgggaccagg acgaaccggg caacagtcgc tggattctgg 300
aaagctaccg gacgtgacaa aatcatctgc agttgtgtcc ggagaattgg actgaggaag 360
acactcgtgt tctacaaagg aagagctcct cacggtcaga aatccgactg gatcatgcat 420
gagtatcgcc tcgacgatac tccaatgtct aatggctatg ctgatgttgt tacagaagat 480
ccaatgagct ataacgaaga aggttgggtg gtatgtcgag tgttcaggaa gaagaactat 540
caaaagattg acgattgtcc taaaatcact ctatcttctt tacctgatga cacggaggaa 600
gagaaggggc ccacctttca caacactcaa aacgttaccg gtttagacca tgttcttctc 660
tacatggacc gtaccggttc taacatttgc atgcccgaga gccaaacaac gactcaacat 720
caagatgatg tcttattcat gcaactccca agtcttgaga cacctaaatc cgagagcccg 780
gtcgaccaaa gtttcctgac tccaagcaaa ctcgatttct ctcccgttca agagaagata 840
accgaaagac cggtttgcag caactgggct agtcttgacc ggctcgtagc ttggcaattg 900
aacaatggtc atcataatcc gtgtcatcgt aagagttttg atgaagaaga agaaaatggt 960
gatactatga tgcagcgatg ggatcttcat tggaataatg atgataatgt tgatctttgg 1020
agtagtttca ctgagtcttc ttcgtcttta gacccacttc ttcatttatc tgtatga 1077
<210> 18
<211> 358
<212> PRT
<213> 拟南芥()
<220>
<223> 转录因子NAC次生壁增厚促进因子3 (NST3), SND1/ANAC012
<400> 18
Met Ala Asp Asn Lys Val Asn Leu Ser Ile Asn Gly Gln Ser Lys Val
1 5 10 15
Pro Pro Gly Phe Arg Phe His Pro Thr Glu Glu Glu Leu Leu His Tyr
20 25 30
Tyr Leu Arg Lys Lys Val Asn Ser Gln Lys Ile Asp Leu Asp Val Ile
35 40 45
Arg Glu Val Asp Leu Asn Lys Leu Glu Pro Trp Asp Ile Gln Glu Glu
50 55 60
Cys Arg Ile Gly Ser Thr Pro Gln Asn Asp Trp Tyr Phe Phe Ser His
65 70 75 80
Lys Asp Lys Lys Tyr Pro Thr Gly Thr Arg Thr Asn Arg Ala Thr Val
85 90 95
Ala Gly Phe Trp Lys Ala Thr Gly Arg Asp Lys Ile Ile Cys Ser Cys
100 105 110
Val Arg Arg Ile Gly Leu Arg Lys Thr Leu Val Phe Tyr Lys Gly Arg
115 120 125
Ala Pro His Gly Gln Lys Ser Asp Trp Ile Met His Glu Tyr Arg Leu
130 135 140
Asp Asp Thr Pro Met Ser Asn Gly Tyr Ala Asp Val Val Thr Glu Asp
145 150 155 160
Pro Met Ser Tyr Asn Glu Glu Gly Trp Val Val Cys Arg Val Phe Arg
165 170 175
Lys Lys Asn Tyr Gln Lys Ile Asp Asp Cys Pro Lys Ile Thr Leu Ser
180 185 190
Ser Leu Pro Asp Asp Thr Glu Glu Glu Lys Gly Pro Thr Phe His Asn
195 200 205
Thr Gln Asn Val Thr Gly Leu Asp His Val Leu Leu Tyr Met Asp Arg
210 215 220
Thr Gly Ser Asn Ile Cys Met Pro Glu Ser Gln Thr Thr Thr Gln His
225 230 235 240
Gln Asp Asp Val Leu Phe Met Gln Leu Pro Ser Leu Glu Thr Pro Lys
245 250 255
Ser Glu Ser Pro Val Asp Gln Ser Phe Leu Thr Pro Ser Lys Leu Asp
260 265 270
Phe Ser Pro Val Gln Glu Lys Ile Thr Glu Arg Pro Val Cys Ser Asn
275 280 285
Trp Ala Ser Leu Asp Arg Leu Val Ala Trp Gln Leu Asn Asn Gly His
290 295 300
His Asn Pro Cys His Arg Lys Ser Phe Asp Glu Glu Glu Glu Asn Gly
305 310 315 320
Asp Thr Met Met Gln Arg Trp Asp Leu His Trp Asn Asn Asp Asp Asn
325 330 335
Val Asp Leu Trp Ser Ser Phe Thr Glu Ser Ser Ser Ser Leu Asp Pro
340 345 350
Leu Leu His Leu Ser Val
355
<210> 19
<211> 918
<212> DNA
<213> 拟南芥()
<220>
<223> 次生壁相关的NAC结构域蛋白2 (SND2), ANAC073, At4g28500
<400> 19
atgacttggt gcaatgaccg tagcgatgtt cagaccgttg aaagaatcat tccctccccg 60
ggggcggctg agtcccccgt agcctcactt ccggtctctt gtcacaaaac ttgcccttct 120
tgtggccata acttcaagtt tcacgaacag gctgggatcc atgacttgcc gggacttcct 180
gctggagtaa aatttgatcc gacggatcaa gaggtcttgg agcatcttga aggcaaggta 240
agagatgacg caaaaaagct tcatcctctc attgatgagt ttatccgtac catcgatggt 300
gaaaacggca tttgttatac ccatcctgaa aaattgccag gagtgaacaa ggacgggacg 360
gtgcgtcatt tcttccaccg accgtcgaag gcatacacga cgggaacaag aaagcgacgt 420
aaagtccaca ctgattctga cgtcggtgga gagacacggt ggcacaagac aggaaaaaca 480
cggccagttc ttgctggagg aagagtgaga ggctacaaga aaatcctagt gctctacaca 540
aactacggca aacaaaaaaa acccgagaag actaattggg taatgcatca atatcatctt 600
ggcactagcg aggaagagaa agaaggtgag ctcgtcgtct ccaaagtctt ttaccagact 660
caaccacgtc aatgcggtgg ctccgttgct gctgcagcca ccgctaagga ccgaccttac 720
ctccacggcc tcggtggagg tggtggccgc caccttcatt accatcttca tcataacaac 780
ggtaacggta agagcaacgg cagtggggga accgccggag ccggtgagta ttatcacaat 840
attccggcta ttatctcgtt caatcagacc gggatacaga accacttggt tcatgactct 900
caacctttta tcccttaa 918
<210> 20
<211> 305
<212> PRT
<213> 拟南芥()
<220>
<223> 次生壁相关的NAC结构域蛋白2 (SND2), ANAC073
<400> 20
Met Thr Trp Cys Asn Asp Arg Ser Asp Val Gln Thr Val Glu Arg Ile
1 5 10 15
Ile Pro Ser Pro Gly Ala Ala Glu Ser Pro Val Ala Ser Leu Pro Val
20 25 30
Ser Cys His Lys Thr Cys Pro Ser Cys Gly His Asn Phe Lys Phe His
35 40 45
Glu Gln Ala Gly Ile His Asp Leu Pro Gly Leu Pro Ala Gly Val Lys
50 55 60
Phe Asp Pro Thr Asp Gln Glu Val Leu Glu His Leu Glu Gly Lys Val
65 70 75 80
Arg Asp Asp Ala Lys Lys Leu His Pro Leu Ile Asp Glu Phe Ile Arg
85 90 95
Thr Ile Asp Gly Glu Asn Gly Ile Cys Tyr Thr His Pro Glu Lys Leu
100 105 110
Pro Gly Val Asn Lys Asp Gly Thr Val Arg His Phe Phe His Arg Pro
115 120 125
Ser Lys Ala Tyr Thr Thr Gly Thr Arg Lys Arg Arg Lys Val His Thr
130 135 140
Asp Ser Asp Val Gly Gly Glu Thr Arg Trp His Lys Thr Gly Lys Thr
145 150 155 160
Arg Pro Val Leu Ala Gly Gly Arg Val Arg Gly Tyr Lys Lys Ile Leu
165 170 175
Val Leu Tyr Thr Asn Tyr Gly Lys Gln Lys Lys Pro Glu Lys Thr Asn
180 185 190
Trp Val Met His Gln Tyr His Leu Gly Thr Ser Glu Glu Glu Lys Glu
195 200 205
Gly Glu Leu Val Val Ser Lys Val Phe Tyr Gln Thr Gln Pro Arg Gln
210 215 220
Cys Gly Gly Ser Val Ala Ala Ala Ala Thr Ala Lys Asp Arg Pro Tyr
225 230 235 240
Leu His Gly Leu Gly Gly Gly Gly Gly Arg His Leu His Tyr His Leu
245 250 255
His His Asn Asn Gly Asn Gly Lys Ser Asn Gly Ser Gly Gly Thr Ala
260 265 270
Gly Ala Gly Glu Tyr Tyr His Asn Ile Pro Ala Ile Ile Ser Phe Asn
275 280 285
Gln Thr Gly Ile Gln Asn His Leu Val His Asp Ser Gln Pro Phe Ile
290 295 300
Pro
305
<210> 21
<211> 945
<212> DNA
<213> 拟南芥()
<220>
<223> 次生壁相关的NAC结构域蛋白3 (SND3), ANAC010, At1g28470
<400> 21
atgagttggt gtgatggttc agatgataac tacgatctta atcttgaaag agtatcgaac 60
actgatcatc catcggttca actcaaagac caatctcaat catgtgtaac gagccgtcca 120
gattccaaga ttagcgctga aactcccatc acgacttgtc cttcttgcgg acacaagctc 180
catcatcacc aagacgacca ggttggtagc atcaaagatt taccaagctt accggcagga 240
gtcaaattcg atccgtcgga taaagagatc cttatgcatt tggaggcgaa ggtatcatcc 300
gataagcgaa aacttcatcc gttgattgat gaatttatac ctacgcttga aggagagaat 360
ggaatttgtt atacgcatcc tgagaaactt cctggagtaa gcaaggacgg gcaagtacgg 420
cacttcttcc accggccatc aaaggcttat acgaccggaa cacgaaaacg aagaaaagtg 480
agcacagatg aggaaggcca tgaaacaagg tggcacaaaa caggcaagac tcgacctgtt 540
ttgtctcaat caggagaaac cggtttcaag aagatcctag tgctctacac caactatggt 600
cgccagaaga agcctgagaa gacgaattgg gtgatgcatc agtatcattt aggtagcagc 660
gaggacgaaa aagacggtga accagtcctc tctaaagtct tctaccaaac acagcctagg 720
caatgcggtt cgatggaacc taaaccgaaa aatctcgtaa acctaaaccg gtttagttat 780
gaaaatattc aggccggttt cgggtatgag catggtggta aaagtgaaga gacgacgcag 840
gtgattcgag agttggtagt tcgtgaaggc gatgggtcat gttcgtttct tagttttact 900
tgtgatgcaa gtaagggtaa agaaagcttc atgaagaatc aatag 945
<210> 22
<211> 314
<212> PRT
<213> 拟南芥()
<220>
<223> 次生壁相关的NAC结构域蛋白3 (SND3), ANAC010
<400> 22
Met Ser Trp Cys Asp Gly Ser Asp Asp Asn Tyr Asp Leu Asn Leu Glu
1 5 10 15
Arg Val Ser Asn Thr Asp His Pro Ser Val Gln Leu Lys Asp Gln Ser
20 25 30
Gln Ser Cys Val Thr Ser Arg Pro Asp Ser Lys Ile Ser Ala Glu Thr
35 40 45
Pro Ile Thr Thr Cys Pro Ser Cys Gly His Lys Leu His His His Gln
50 55 60
Asp Asp Gln Val Gly Ser Ile Lys Asp Leu Pro Ser Leu Pro Ala Gly
65 70 75 80
Val Lys Phe Asp Pro Ser Asp Lys Glu Ile Leu Met His Leu Glu Ala
85 90 95
Lys Val Ser Ser Asp Lys Arg Lys Leu His Pro Leu Ile Asp Glu Phe
100 105 110
Ile Pro Thr Leu Glu Gly Glu Asn Gly Ile Cys Tyr Thr His Pro Glu
115 120 125
Lys Leu Pro Gly Val Ser Lys Asp Gly Gln Val Arg His Phe Phe His
130 135 140
Arg Pro Ser Lys Ala Tyr Thr Thr Gly Thr Arg Lys Arg Arg Lys Val
145 150 155 160
Ser Thr Asp Glu Glu Gly His Glu Thr Arg Trp His Lys Thr Gly Lys
165 170 175
Thr Arg Pro Val Leu Ser Gln Ser Gly Glu Thr Gly Phe Lys Lys Ile
180 185 190
Leu Val Leu Tyr Thr Asn Tyr Gly Arg Gln Lys Lys Pro Glu Lys Thr
195 200 205
Asn Trp Val Met His Gln Tyr His Leu Gly Ser Ser Glu Asp Glu Lys
210 215 220
Asp Gly Glu Pro Val Leu Ser Lys Val Phe Tyr Gln Thr Gln Pro Arg
225 230 235 240
Gln Cys Gly Ser Met Glu Pro Lys Pro Lys Asn Leu Val Asn Leu Asn
245 250 255
Arg Phe Ser Tyr Glu Asn Ile Gln Ala Gly Phe Gly Tyr Glu His Gly
260 265 270
Gly Lys Ser Glu Glu Thr Thr Gln Val Ile Arg Glu Leu Val Val Arg
275 280 285
Glu Gly Asp Gly Ser Cys Ser Phe Leu Ser Phe Thr Cys Asp Ala Ser
290 295 300
Lys Gly Lys Glu Ser Phe Met Lys Asn Gln
305 310
<210> 23
<211> 1113
<212> DNA
<213> 拟南芥()
<220>
<223> MYB结构域蛋白103 (MYB103), At1g63910
<400> 23
atgggtcatc actcatgctg caaccagcaa aaggtgaaga gagggctttg gtcaccggaa 60
gaagatgaga agcttattag atatatcaca actcatggct atggatgttg gagtgaagtc 120
cctgaaaaag cagggcttca aagatgtgga aaaagttgta gattgcgatg gataaactat 180
cttcgacctg atatcaggag aggaaggttc tctccagaag aagagaaatt gatcataagc 240
cttcatggag ttgtgggaaa caggtgggct catatagcta gtcatttacc gggaagaaca 300
gataacgaga ttaaaaacta ttggaattca tggattaaga aaaagatacg aaaaccgcac 360
catcattaca gtcgtcatca accgtcagta actactgtga cattgaatgc ggacactaca 420
tcgattgcca ctaccatcga ggcctctacc accacaacat cgactatcga taacttacat 480
tttgacggtt tcactgattc tcctaaccaa ttaaatttca ccaatgatca agaaactaat 540
ataaagattc aagaaacttt tttctcccat aaacctcctc tcttcatggt agacacaaca 600
cttcctatcc tagaaggaat gttctctgaa aacatcatca caaacaataa caagaacaat 660
gatcatgatg acacgcaaag aggaggaaga gaaaatgttt gtgaacaagc atttctaaca 720
actaacacgg aagaatggga tatgaatctt cgtcagcaag agccgtttca agttcctaca 780
ctggcgtcac atgtgttcaa caactcttcc aattcaaata ttgacacggt tataagttat 840
aatctaccgg cgctaataga gggaaatgtc gataacatcg tccataatga aaacagcaat 900
gtccaagatg gagaaatggc gtccacattc gaatgtttaa agaggcaaga actaagctat 960
gatcaatggg acgattcaca acaatgctct aactttttct tttgggacaa ccttaatata 1020
aacgtggaag gttcatctct tgttggaaac caagacccat caatgaattt gggatcatct 1080
gccttatctt cttctttccc ttcttcgttt taa 1113
<210> 24
<211> 370
<212> PRT
<213> 拟南芥()
<220>
<223> MYB结构域蛋白103 (MYB103)
<400> 24
Met Gly His His Ser Cys Cys Asn Gln Gln Lys Val Lys Arg Gly Leu
1 5 10 15
Trp Ser Pro Glu Glu Asp Glu Lys Leu Ile Arg Tyr Ile Thr Thr His
20 25 30
Gly Tyr Gly Cys Trp Ser Glu Val Pro Glu Lys Ala Gly Leu Gln Arg
35 40 45
Cys Gly Lys Ser Cys Arg Leu Arg Trp Ile Asn Tyr Leu Arg Pro Asp
50 55 60
Ile Arg Arg Gly Arg Phe Ser Pro Glu Glu Glu Lys Leu Ile Ile Ser
65 70 75 80
Leu His Gly Val Val Gly Asn Arg Trp Ala His Ile Ala Ser His Leu
85 90 95
Pro Gly Arg Thr Asp Asn Glu Ile Lys Asn Tyr Trp Asn Ser Trp Ile
100 105 110
Lys Lys Lys Ile Arg Lys Pro His His His Tyr Ser Arg His Gln Pro
115 120 125
Ser Val Thr Thr Val Thr Leu Asn Ala Asp Thr Thr Ser Ile Ala Thr
130 135 140
Thr Ile Glu Ala Ser Thr Thr Thr Thr Ser Thr Ile Asp Asn Leu His
145 150 155 160
Phe Asp Gly Phe Thr Asp Ser Pro Asn Gln Leu Asn Phe Thr Asn Asp
165 170 175
Gln Glu Thr Asn Ile Lys Ile Gln Glu Thr Phe Phe Ser His Lys Pro
180 185 190
Pro Leu Phe Met Val Asp Thr Thr Leu Pro Ile Leu Glu Gly Met Phe
195 200 205
Ser Glu Asn Ile Ile Thr Asn Asn Asn Lys Asn Asn Asp His Asp Asp
210 215 220
Thr Gln Arg Gly Gly Arg Glu Asn Val Cys Glu Gln Ala Phe Leu Thr
225 230 235 240
Thr Asn Thr Glu Glu Trp Asp Met Asn Leu Arg Gln Gln Glu Pro Phe
245 250 255
Gln Val Pro Thr Leu Ala Ser His Val Phe Asn Asn Ser Ser Asn Ser
260 265 270
Asn Ile Asp Thr Val Ile Ser Tyr Asn Leu Pro Ala Leu Ile Glu Gly
275 280 285
Asn Val Asp Asn Ile Val His Asn Glu Asn Ser Asn Val Gln Asp Gly
290 295 300
Glu Met Ala Ser Thr Phe Glu Cys Leu Lys Arg Gln Glu Leu Ser Tyr
305 310 315 320
Asp Gln Trp Asp Asp Ser Gln Gln Cys Ser Asn Phe Phe Phe Trp Asp
325 330 335
Asn Leu Asn Ile Asn Val Glu Gly Ser Ser Leu Val Gly Asn Gln Asp
340 345 350
Pro Ser Met Asn Leu Gly Ser Ser Ala Leu Ser Ser Ser Phe Pro Ser
355 360 365
Ser Phe
370
<210> 25
<211> 801
<212> DNA
<213> 拟南芥()
<220>
<223> MYB结构域蛋白85 (MYB85), At4g22680
<400> 25
atggggagac agccatgctg tgacaagcta ggggtgaaga aagggccgtg gacggtggag 60
gaagataaga agcttataaa cttcatacta accaatggcc attgttgctg gcgtgctttg 120
ccgaagctgg ccggtctccg tcgctgtgga aagagctgcc gcctccggtg gactaactat 180
ctccggcctg acttaaaacg aggccttctc tcgcatgatg aagaacaact tgtcatagat 240
cttcatgcta atctcggcaa taagtggtct aagatagctt caagattacc tggaagaaca 300
gataacgaaa taaaaaacca ttggaatact catatcaaga agaaacttct taagatggga 360
atcgatccta tgacccatca acccctaaat caagaacctt ctaatatcga taattccaaa 420
accattccgt ccaatccaga cgatgtctca gtggaaccaa agacaactaa cacgaaatac 480
gtggagataa gtgtcacgac aacagaagaa gaaagtagta gcacggttac tgatcaaaac 540
agttcgatgg ataatgaaaa tcatctaatt gacaacattt atgatgatga tgaattgttt 600
agttacttat ggtccgacga aactactaaa gatgaggcct cttggagtga tagtaacttt 660
ggtgttggtg gaacattata tgaccacaat atctccggcg ccgatgcaga ttttccgata 720
tggtcaccgg aaagaatcaa tgacgagaag atgtttttgg attattgtca agactttggt 780
gttcatgatt ttgggttttg a 801
<210> 26
<211> 266
<212> PRT
<213> 拟南芥()
<220>
<223> MYB结构域蛋白85 (MYB85)
<400> 26
Met Gly Arg Gln Pro Cys Cys Asp Lys Leu Gly Val Lys Lys Gly Pro
1 5 10 15
Trp Thr Val Glu Glu Asp Lys Lys Leu Ile Asn Phe Ile Leu Thr Asn
20 25 30
Gly His Cys Cys Trp Arg Ala Leu Pro Lys Leu Ala Gly Leu Arg Arg
35 40 45
Cys Gly Lys Ser Cys Arg Leu Arg Trp Thr Asn Tyr Leu Arg Pro Asp
50 55 60
Leu Lys Arg Gly Leu Leu Ser His Asp Glu Glu Gln Leu Val Ile Asp
65 70 75 80
Leu His Ala Asn Leu Gly Asn Lys Trp Ser Lys Ile Ala Ser Arg Leu
85 90 95
Pro Gly Arg Thr Asp Asn Glu Ile Lys Asn His Trp Asn Thr His Ile
100 105 110
Lys Lys Lys Leu Leu Lys Met Gly Ile Asp Pro Met Thr His Gln Pro
115 120 125
Leu Asn Gln Glu Pro Ser Asn Ile Asp Asn Ser Lys Thr Ile Pro Ser
130 135 140
Asn Pro Asp Asp Val Ser Val Glu Pro Lys Thr Thr Asn Thr Lys Tyr
145 150 155 160
Val Glu Ile Ser Val Thr Thr Thr Glu Glu Glu Ser Ser Ser Thr Val
165 170 175
Thr Asp Gln Asn Ser Ser Met Asp Asn Glu Asn His Leu Ile Asp Asn
180 185 190
Ile Tyr Asp Asp Asp Glu Leu Phe Ser Tyr Leu Trp Ser Asp Glu Thr
195 200 205
Thr Lys Asp Glu Ala Ser Trp Ser Asp Ser Asn Phe Gly Val Gly Gly
210 215 220
Thr Leu Tyr Asp His Asn Ile Ser Gly Ala Asp Ala Asp Phe Pro Ile
225 230 235 240
Trp Ser Pro Glu Arg Ile Asn Asp Glu Lys Met Phe Leu Asp Tyr Cys
245 250 255
Gln Asp Phe Gly Val His Asp Phe Gly Phe
260 265
<210> 27
<211> 843
<212> DNA
<213> 拟南芥()
<220>
<223> MYB结构域蛋白46 (MYB46), At5g12870
<400> 27
atgaggaagc cagaggtagc cattgcagct agtactcacc aagtaaagaa gatgaagaag 60
ggactttggt ctcctgagga agactcaaag ctgatgcaat acatgttaag caatggacaa 120
ggatgttgga gtgatgttgc gaaaaacgca ggacttcaaa gatgtggcaa aagctgccgt 180
cttcgttgga tcaactatct tcgtcctgac ctcaagcgtg gcgctttctc tcctcaagaa 240
gaggatctca tcattcgctt tcattccatc ctcggcaaca ggtggtctca gattgcagca 300
cgattgcctg gtcggaccga taacgagatc aagaatttct ggaactcaac aataaagaaa 360
aggctaaaga agatgtccga tacctccaac ttaatcaaca actcatcctc atcacccaac 420
acagcaagcg attcctcttc taattccgca tcttctttgg atattaaaga cattatagga 480
agcttcatgt ccttacaaga acaaggcttc gtcaaccctt ccttgaccca catacaaacc 540
aacaatccat ttccaacggg aaacatgatc agccacccgt gcaatgacga ttttacccct 600
tatgtagatg gtatctatgg agtaaacgca ggggtacaag gggaactcta cttcccacct 660
ttggaatgtg aagaaggtga ttggtacaat gcaaatataa acaaccactt agacgagttg 720
aacactaatg gatccggaaa cgcacctgag ggtatgagac cagtggaaga attttgggac 780
cttgaccagt tgatgaacac tgaggttcct tcgttttact tcaacttcaa acaaagcata 840
tga 843
<210> 28
<211> 280
<212> PRT
<213> 拟南芥()
<220>
<223> MYB结构域蛋白46 (MYB46)
<400> 28
Met Arg Lys Pro Glu Val Ala Ile Ala Ala Ser Thr His Gln Val Lys
1 5 10 15
Lys Met Lys Lys Gly Leu Trp Ser Pro Glu Glu Asp Ser Lys Leu Met
20 25 30
Gln Tyr Met Leu Ser Asn Gly Gln Gly Cys Trp Ser Asp Val Ala Lys
35 40 45
Asn Ala Gly Leu Gln Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Ile
50 55 60
Asn Tyr Leu Arg Pro Asp Leu Lys Arg Gly Ala Phe Ser Pro Gln Glu
65 70 75 80
Glu Asp Leu Ile Ile Arg Phe His Ser Ile Leu Gly Asn Arg Trp Ser
85 90 95
Gln Ile Ala Ala Arg Leu Pro Gly Arg Thr Asp Asn Glu Ile Lys Asn
100 105 110
Phe Trp Asn Ser Thr Ile Lys Lys Arg Leu Lys Lys Met Ser Asp Thr
115 120 125
Ser Asn Leu Ile Asn Asn Ser Ser Ser Ser Pro Asn Thr Ala Ser Asp
130 135 140
Ser Ser Ser Asn Ser Ala Ser Ser Leu Asp Ile Lys Asp Ile Ile Gly
145 150 155 160
Ser Phe Met Ser Leu Gln Glu Gln Gly Phe Val Asn Pro Ser Leu Thr
165 170 175
His Ile Gln Thr Asn Asn Pro Phe Pro Thr Gly Asn Met Ile Ser His
180 185 190
Pro Cys Asn Asp Asp Phe Thr Pro Tyr Val Asp Gly Ile Tyr Gly Val
195 200 205
Asn Ala Gly Val Gln Gly Glu Leu Tyr Phe Pro Pro Leu Glu Cys Glu
210 215 220
Glu Gly Asp Trp Tyr Asn Ala Asn Ile Asn Asn His Leu Asp Glu Leu
225 230 235 240
Asn Thr Asn Gly Ser Gly Asn Ala Pro Glu Gly Met Arg Pro Val Glu
245 250 255
Glu Phe Trp Asp Leu Asp Gln Leu Met Asn Thr Glu Val Pro Ser Phe
260 265 270
Tyr Phe Asn Phe Lys Gln Ser Ile
275 280
<210> 29
<211> 1032
<212> DNA
<213> 拟南芥()
<220>
<223> MYB结构域蛋白83 (MYB83), At3g08500
<400> 29
atgatgatga ggaaaccgga cattactacg atcagagaca aaggcaagcc aaatcatgca 60
tgtggtggta ataacaacaa accgaagcta agaaaaggac tttggtcgcc tgatgaagat 120
gagaagctga taagatacat gttgactaat ggacaaggat gttggagtga catcgctaga 180
aatgctggtc ttttacgttg tggtaaaagt tgtcgccttc gctggatcaa ttacttgagg 240
cctgatctta aacgtggatc cttctctcct caggaggagg atctcatctt ccatttgcat 300
tccattcttg gtaacaggtg gtctcaaata gctactcggc ttccaggtag aacagacaac 360
gagatcaaaa acttttggaa ctcgacattg aagaagcggc ttaagaacaa cagcaacaac 420
aatacttcat caggatcatc acctaacaat agtaatagta attccttgga cccaagagat 480
caacatgtgg atatgggagg caactcaact tcattgatgg atgactatca tcatgatgaa 540
aacatgatga cagtggggaa caccatgcgc atggactctt cctccccatt caatgttgga 600
ccaatggtta atagtgtggg cttaaaccaa ctttatgatc ccttgatgat atcagtgccg 660
gataacggat atcaccaaat gggaaacaca gtgaatgtgt tcagcgttaa tggtttagga 720
gattatggaa acacaattct tgatccaatt agcaagagag tatcagtaga aggtgatgat 780
tggttcattc ccccctcgga gaataccaac gtcattgctt gtagtacaag caacaaccta 840
aacttacagg cccttgatcc ttgcttcaat agcaaaaatc tttgtcattc agaaagcttc 900
aaggtaggga atgtgttggg gatagagaat ggttcttggg aaatagaaaa ccctaaaatc 960
ggagattggg atttggatgg tctcatcgat aacaactctt cttttccctt ccttgatttc 1020
caagtcgatt ga 1032
<210> 30
<211> 343
<212> PRT
<213> 拟南芥()
<220>
<223> MYB结构域蛋白83 (MYB83)
<400> 30
Met Met Met Arg Lys Pro Asp Ile Thr Thr Ile Arg Asp Lys Gly Lys
1 5 10 15
Pro Asn His Ala Cys Gly Gly Asn Asn Asn Lys Pro Lys Leu Arg Lys
20 25 30
Gly Leu Trp Ser Pro Asp Glu Asp Glu Lys Leu Ile Arg Tyr Met Leu
35 40 45
Thr Asn Gly Gln Gly Cys Trp Ser Asp Ile Ala Arg Asn Ala Gly Leu
50 55 60
Leu Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Ile Asn Tyr Leu Arg
65 70 75 80
Pro Asp Leu Lys Arg Gly Ser Phe Ser Pro Gln Glu Glu Asp Leu Ile
85 90 95
Phe His Leu His Ser Ile Leu Gly Asn Arg Trp Ser Gln Ile Ala Thr
100 105 110
Arg Leu Pro Gly Arg Thr Asp Asn Glu Ile Lys Asn Phe Trp Asn Ser
115 120 125
Thr Leu Lys Lys Arg Leu Lys Asn Asn Ser Asn Asn Asn Thr Ser Ser
130 135 140
Gly Ser Ser Pro Asn Asn Ser Asn Ser Asn Ser Leu Asp Pro Arg Asp
145 150 155 160
Gln His Val Asp Met Gly Gly Asn Ser Thr Ser Leu Met Asp Asp Tyr
165 170 175
His His Asp Glu Asn Met Met Thr Val Gly Asn Thr Met Arg Met Asp
180 185 190
Ser Ser Ser Pro Phe Asn Val Gly Pro Met Val Asn Ser Val Gly Leu
195 200 205
Asn Gln Leu Tyr Asp Pro Leu Met Ile Ser Val Pro Asp Asn Gly Tyr
210 215 220
His Gln Met Gly Asn Thr Val Asn Val Phe Ser Val Asn Gly Leu Gly
225 230 235 240
Asp Tyr Gly Asn Thr Ile Leu Asp Pro Ile Ser Lys Arg Val Ser Val
245 250 255
Glu Gly Asp Asp Trp Phe Ile Pro Pro Ser Glu Asn Thr Asn Val Ile
260 265 270
Ala Cys Ser Thr Ser Asn Asn Leu Asn Leu Gln Ala Leu Asp Pro Cys
275 280 285
Phe Asn Ser Lys Asn Leu Cys His Ser Glu Ser Phe Lys Val Gly Asn
290 295 300
Val Leu Gly Ile Glu Asn Gly Ser Trp Glu Ile Glu Asn Pro Lys Ile
305 310 315 320
Gly Asp Trp Asp Leu Asp Gly Leu Ile Asp Asn Asn Ser Ser Phe Pro
325 330 335
Phe Leu Asp Phe Gln Val Asp
340
<210> 31
<211> 825
<212> DNA
<213> 拟南芥()
<220>
<223> MYB结构域蛋白58 (MYB58), At1g16490
<400> 31
atgggcaaag gaagagcacc atgttgtgac aaaaccaaag tgaagagagg accatggagc 60
catgatgaag acttgaaact catctctttc attcacaaga atggtcatga gaattggaga 120
tctctcccaa agcaagctgg attgttgagg tgtggcaaga gttgtcgtct gcgatggatt 180
aattacctca gacctgatgt gaaacgtggc aatttcagtg cagaggaaga agacaccatc 240
atcaaacttc accagagctt tggtaacaag tggtcgaaga ttgcttctaa gctgcctgga 300
agaacagaca atgagatcaa gaatgtgtgg catacacatc tcaagaaaag attgagctcg 360
gaaactaacc ttaatgccga tgaagcgggt tcaaaaggtt ctttgaatga agaagagaac 420
tctcaagagt catctccaaa tgcttcaatg tcttttgctg gttccaacat ttcaagcaaa 480
gacgatgatg cacagataag tcaaatgttt gagcacattc taacttatag cgagtttacg 540
gggatgttac aagaggtaga caaaccagag ctgctggaga tgccttttga tttagatcct 600
gacatttgga gtttcataga tggttcagac tcattccaac aaccagagaa cagagctctt 660
caagagtctg aagaagatga agttgataaa tggtttaagc acctggaaag cgaactcggg 720
ttagaagaaa acgataacca acaacaacaa caacagcata aacagggaac agaagatgaa 780
cattcatcat cactcttgga gagttacgag ctcctcatac attaa 825
<210> 32
<211> 274
<212> PRT
<213> 拟南芥()
<220>
<223> MYB结构域蛋白58 (MYB58)
<400> 32
Met Gly Lys Gly Arg Ala Pro Cys Cys Asp Lys Thr Lys Val Lys Arg
1 5 10 15
Gly Pro Trp Ser His Asp Glu Asp Leu Lys Leu Ile Ser Phe Ile His
20 25 30
Lys Asn Gly His Glu Asn Trp Arg Ser Leu Pro Lys Gln Ala Gly Leu
35 40 45
Leu Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Ile Asn Tyr Leu Arg
50 55 60
Pro Asp Val Lys Arg Gly Asn Phe Ser Ala Glu Glu Glu Asp Thr Ile
65 70 75 80
Ile Lys Leu His Gln Ser Phe Gly Asn Lys Trp Ser Lys Ile Ala Ser
85 90 95
Lys Leu Pro Gly Arg Thr Asp Asn Glu Ile Lys Asn Val Trp His Thr
100 105 110
His Leu Lys Lys Arg Leu Ser Ser Glu Thr Asn Leu Asn Ala Asp Glu
115 120 125
Ala Gly Ser Lys Gly Ser Leu Asn Glu Glu Glu Asn Ser Gln Glu Ser
130 135 140
Ser Pro Asn Ala Ser Met Ser Phe Ala Gly Ser Asn Ile Ser Ser Lys
145 150 155 160
Asp Asp Asp Ala Gln Ile Ser Gln Met Phe Glu His Ile Leu Thr Tyr
165 170 175
Ser Glu Phe Thr Gly Met Leu Gln Glu Val Asp Lys Pro Glu Leu Leu
180 185 190
Glu Met Pro Phe Asp Leu Asp Pro Asp Ile Trp Ser Phe Ile Asp Gly
195 200 205
Ser Asp Ser Phe Gln Gln Pro Glu Asn Arg Ala Leu Gln Glu Ser Glu
210 215 220
Glu Asp Glu Val Asp Lys Trp Phe Lys His Leu Glu Ser Glu Leu Gly
225 230 235 240
Leu Glu Glu Asn Asp Asn Gln Gln Gln Gln Gln Gln His Lys Gln Gly
245 250 255
Thr Glu Asp Glu His Ser Ser Ser Leu Leu Glu Ser Tyr Glu Leu Leu
260 265 270
Ile His
<210> 33
<211> 885
<212> DNA
<213> 拟南芥()
<220>
<223> MYB结构域蛋白63 (MYB63), At1g79180
<400> 33
atggggaagg gaagagcacc ttgttgtgac aagaccaaag tgaagagagg tccatggagc 60
ccagaagaag acattaaact catctctttc attcaaaagt ttggtcatga gaactggaga 120
tctctcccca aacaatctgg gctattgagg tgtgggaaga gttgtcgtct aaggtggatt 180
aactatctta ggccagatct gaagcgtggc aacttcactt cagaggagga agaaacaatc 240
attaagcttc accacaacta tgggaacaag tggtcgaaaa tcgcttctca acttccaggt 300
agaacagata acgagatcaa gaatgtgtgg cacactcatc taaagaaaag actggctcag 360
agctcaggaa ctgcagatga accggcctcg ccttgttcga gtgattctgt ttctcgtggg 420
aaagatgata agtcatctca cgtagaagat tctttgaaca gagagactaa tcataggaat 480
gagttgtcta catctatgtc ttctgggggt tccaaccaac aagatgatcc aaagatagac 540
gaactcaggt ttgagtatat agaagaagct tatagcgagt ttaacgacat tattattcaa 600
gaggtagaca aacccgatct gctggagata ccatttgatt cagatcctga catttggagt 660
ttcttagata cttcaaactc atttcaacaa tccactgcaa atgagaacag ctcaggctca 720
agagcaacaa cagaagaaga gtctgatgag gatgaggtta agaaatggtt caagcaccta 780
gaaagcgaac tcgggttaga agaagacgat aatcaacaac aatacaaaga agaagaatca 840
tcatcatcat cactcttgaa gaactacgag ctcatgatac attga 885
<210> 34
<211> 294
<212> PRT
<213> 拟南芥()
<220>
<223> MYB结构域蛋白63 (MYB63)
<400> 34
Met Gly Lys Gly Arg Ala Pro Cys Cys Asp Lys Thr Lys Val Lys Arg
1 5 10 15
Gly Pro Trp Ser Pro Glu Glu Asp Ile Lys Leu Ile Ser Phe Ile Gln
20 25 30
Lys Phe Gly His Glu Asn Trp Arg Ser Leu Pro Lys Gln Ser Gly Leu
35 40 45
Leu Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Ile Asn Tyr Leu Arg
50 55 60
Pro Asp Leu Lys Arg Gly Asn Phe Thr Ser Glu Glu Glu Glu Thr Ile
65 70 75 80
Ile Lys Leu His His Asn Tyr Gly Asn Lys Trp Ser Lys Ile Ala Ser
85 90 95
Gln Leu Pro Gly Arg Thr Asp Asn Glu Ile Lys Asn Val Trp His Thr
100 105 110
His Leu Lys Lys Arg Leu Ala Gln Ser Ser Gly Thr Ala Asp Glu Pro
115 120 125
Ala Ser Pro Cys Ser Ser Asp Ser Val Ser Arg Gly Lys Asp Asp Lys
130 135 140
Ser Ser His Val Glu Asp Ser Leu Asn Arg Glu Thr Asn His Arg Asn
145 150 155 160
Glu Leu Ser Thr Ser Met Ser Ser Gly Gly Ser Asn Gln Gln Asp Asp
165 170 175
Pro Lys Ile Asp Glu Leu Arg Phe Glu Tyr Ile Glu Glu Ala Tyr Ser
180 185 190
Glu Phe Asn Asp Ile Ile Ile Gln Glu Val Asp Lys Pro Asp Leu Leu
195 200 205
Glu Ile Pro Phe Asp Ser Asp Pro Asp Ile Trp Ser Phe Leu Asp Thr
210 215 220
Ser Asn Ser Phe Gln Gln Ser Thr Ala Asn Glu Asn Ser Ser Gly Ser
225 230 235 240
Arg Ala Thr Thr Glu Glu Glu Ser Asp Glu Asp Glu Val Lys Lys Trp
245 250 255
Phe Lys His Leu Glu Ser Glu Leu Gly Leu Glu Glu Asp Asp Asn Gln
260 265 270
Gln Gln Tyr Lys Glu Glu Glu Ser Ser Ser Ser Ser Leu Leu Lys Asn
275 280 285
Tyr Glu Leu Met Ile His
290
<210> 35
<211> 1577
<212> DNA
<213> 拟南芥()
<220>
<221> 启动子
<222> (1)..(.1577)
<223> 木聚糖生物合成酶不规则的木质部8 (IRX8) (GAUT12)组织特异性的次生壁启动子
<400> 35
acgagctgac ttgtaccgat gagctggctc ttctgggcga gctggctgat cttgacgagc 60
agacttctcc cgacgagctg acttgtgtcg atgagctggc tcttctgggc gagttggctg 120
atcttgacga gcagacttct cccgacgagc tgacttgtgt cgatgagctg gctcttctgg 180
gcgaactggc tgatcttgac gagcagactt ctcccgacga gctgacttgt gctatccttt 240
ctccaggtct cgaaaaagtc ccctttcccg agactttcta ttccttattt atacccgtcc 300
gtatagtagg gtacgcaagg tgaattctcg agagtgcccc ttttctacgc agccgaactc 360
acatcctgac caggccgggc ttcggcctgg tgggccggct cgagttctaa agtgatggtc 420
ggggctgggt cgttattcct tgaaatgggc cggttgatca ctgaggccca attgatgtat 480
caacatgtgg tttttataaa aagagtcgtg agaagagttt tctctaaaaa tcccttgtgt 540
ttggtaatca aacttcattc aaccaacgaa ttccaaaaaa acaactaaat tgttcgggta 600
tataaaatga ttggtaatga tatatcccat agaggccgta gacataggcc caaaaagttt 660
ccataactag cagaaattga aacttgcaag ttgcaaatat tattacactg gaaaggcaac 720
aagtcttgaa gtacaaacta caaagacttc ttgtttggat ggggacgact gacgagtttg 780
aataacttaa gagaaaaggg tcgcaatcga aattagacaa gaaattagtc ctcaaaaagt 840
aaattctgaa gttgaagctc caatgtcttt gttcaaagac tttatttaga tgtaaagtta 900
tgtcttgtaa ccaccaaaca gctccttttc atctacactc ccaatttttt taacatctat 960
gttttgcatt gcctttgact tgtctttctc tctccaactt ctctccttca acataaagcc 1020
aaatcctaaa tccaaatccc ttaaaccgaa ccgaattaaa ccgaagctgt tgaactatcg 1080
caaaatttca gatcttacta atcataaaca tgtgacgttt aattcatttt aagagtttca 1140
tgatttgcac tgaatggtat tccgagtcca ccggaaaaaa acttttccta caagtagaaa 1200
aaggataacc ccataaatcc aaataaccta accgatcaaa catataccaa tataaaccaa 1260
aacaagattc agattcatcg gtttagtaat cgaagtaatg tactaatgtg taatattgat 1320
tccaccacca gcttagagat tcgaaccaaa aaccgaatag cgcataaccg agaaaaccca 1380
aagcttccta acaaatacat aaaaccgtgg tgtttctaat tctaaccaac acacgtttcc 1440
tttttattca caagaaacat cagagttatg atctgccatt aataacctaa acacaaagca 1500
aggttaggta aatgatatgg acccctaatg aataatcata caatacataa caacgtaaga 1560
tccagtttcc ctcttcg 1577
<210> 36
<211> 2619
<212> DNA
<213> 拟南芥()
<220>
<221> 启动子
<222> (1)..(.2619)
<223> 维管相关的NAC-结构域蛋白6 (VND6) 导管特异性的启动子
<400> 36
cgccaaaaag atgttatgat gtgatgcatt ctttaatata gattaaacta ttggtgattt 60
gtttttctat agttaatcac tagcaagaac atttttcttt tatgtttaca gtttttagat 120
tataaaaaaa tggtgtaata agaacatgtc aaatcaaatg tatttaattt gttaatatag 180
atttgtgtat aaacaattgg tagttttgat aattcaattt ttcagcaatc atcataataa 240
tactttacaa gaaaagctaa atcatctgaa aattttatat gaatacgtac ggtttaattc 300
ccaatctaca acttttttag ttggatttat tataaccgtt tttctaaaac aaaacacatt 360
aaaatttata agtgaagatc caatgggttc aaacctttta attctcaata aatatacata 420
gattctcgaa gatatcctat caacttgtaa aagttgttta atcaatcttt tgtttgatga 480
aatcttgttc aactgttgat ttggttaagt tttatagctg aaatgtgtat aagtgtttct 540
gaaccttttt aattactgct aaatcaattt atgtcttaca aacttgccga tgtatcatgt 600
atgttcatta ggggtgtcaa aatgagccag ctcgctcagc tcagctcatg atgaacttaa 660
atcttttatg agccagctca gctcaactca tttattatat gagcttcaaa atacaaactc 720
ggactcagat catctagatc acgagctaaa tgaggtagtt cgcgagctaa tgcaaataaa 780
tgaaaaaatt taaattttct aaaattttta tgtaaattat atatttttaa aaatatattt 840
ctatttttat agattatata aatatttatg ttttgattgt tgactttttg tgtttattac 900
taatatttat tttcagaaat tattatgtaa cttatatatt ttctaaaata tatttctatt 960
atttatagaa aaatatatat atactttgat tgttagtttt ttgtatatat tacttcaaaa 1020
aagccaaact catgagctag ctcatgttca ttaaagctcg ctcatataac tcgtgagcta 1080
aataaagttc gatcactaaa ctcatttatt aaatgatcct aaaaaataga attcgcgctc 1140
atgaataact gagtcgagtt gagccagctc atgagctatc agctcatttt gacaccatta 1200
atgttcatat aataatcgta atccatcatg accaattagg caattaagac atactataac 1260
aaaacatttt tttttttttc gtcaaacatt gttttgttaa aggtttcaaa aagaacatgc 1320
gtatcaattt caccaaacga atctaataaa tagcacgacc atcggatatt atattgctat 1380
ttgacgatca acgtacgttc gattaaaaaa agtacgggga ttgtttagtt taatccaagt 1440
gtagtgttat ttagacttcg agtaacatca acgcgggaga agaaacgcat gggatgaatg 1500
tgtaaagtgg ttaactttca acaatgtttc ttaattgcta agatgtttaa aacgagttta 1560
catgaattct tgtgttactt atcgaattat cttttatgag ataattagta cactacctta 1620
ttagggctcc atttcttttt ctatctaggc ctaggtcgat cagtactgtg tatgttacac 1680
atatgatatt aaaaaaaaaa ttgattctat aataatcttc ataactaaag gcaagtatag 1740
tttatttgaa aacgtctcat tcaatagttg gtagagatag ttctaaaaga tcacattact 1800
tctctatcac acagagtgta gaaaagtgaa aacttatgca acacttcagg tagaaagaga 1860
aaagacagtg atagcttagt tataattaaa gacccccaaa atccaaatag aatcttctct 1920
taaataaact attgaaaaaa tattcacaaa aaaataaaaa gcacatttcc ttttgcttgc 1980
atcacgagag cttttgtctc tctttacatt tatgtgccta tatataagcc tagagaatcc 2040
accatcatcg gagtactcgt tgtctttcat gatctcaaca taaacaagac aaaacgcttt 2100
tgtctcttgt ctatctaaat taaatctacg aggaaacgaa gagaagcaac aaaattcgat 2160
attttagaag tcttgcataa ataagaagaa ggtttcaagt aagtttcttt cagtacatag 2220
agaaaccatg tatatgattt atgattgtca agatgttaca cagtcgtata tataattata 2280
agtatttcat aaaagtaatt tatgaaacca tgcacataac taacttttgt ttttgatttt 2340
gtaaattgag tagtttttgt ttttattatc atttttatgt gtttatagtt ggttcagccg 2400
agatattatg agtaaccaaa cgtaaccttt ttcataatga aacggatcaa atatacttta 2460
atctttttcc tacatatgct tagttacttg aaaacttgat ttcacattac ttctatgcat 2520
atcttttcta tgtaccgcgc gatgataaag tatgtgttac aaattgccac attgcagaaa 2580
atataaaatt aaaaagatca aatggaaagt ctcgcacac 2619
<210> 37
<211> 199
<212> PRT
<213> 拟南芥()
<220>
<223> 转录因子shine (SHN1, WIN1), AtSHN1, At1g15360
<400> 37
Met Val Gln Thr Lys Lys Phe Arg Gly Val Arg Gln Arg His Trp Gly
1 5 10 15
Ser Trp Val Ala Glu Ile Arg His Pro Leu Leu Lys Arg Arg Ile Trp
20 25 30
Leu Gly Thr Phe Glu Thr Ala Glu Glu Ala Ala Arg Ala Tyr Asp Glu
35 40 45
Ala Ala Val Leu Met Ser Gly Arg Asn Ala Lys Thr Asn Phe Pro Leu
50 55 60
Asn Asn Asn Asn Thr Gly Glu Thr Ser Glu Gly Lys Thr Asp Ile Ser
65 70 75 80
Ala Ser Ser Thr Met Ser Ser Ser Thr Ser Ser Ser Ser Leu Ser Ser
85 90 95
Ile Leu Ser Ala Lys Leu Arg Lys Cys Cys Lys Ser Pro Ser Pro Ser
100 105 110
Leu Thr Cys Leu Arg Leu Asp Thr Ala Ser Ser His Ile Gly Val Trp
115 120 125
Gln Lys Arg Ala Gly Ser Lys Ser Asp Ser Ser Trp Val Met Thr Val
130 135 140
Glu Leu Gly Pro Ala Ser Ser Ser Gln Glu Thr Thr Ser Lys Ala Ser
145 150 155 160
Gln Asp Ala Ile Leu Ala Pro Thr Thr Glu Val Glu Ile Gly Gly Ser
165 170 175
Arg Glu Glu Val Leu Asp Glu Glu Glu Lys Val Ala Leu Gln Met Ile
180 185 190
Glu Glu Leu Leu Asn Thr Asn
195
<210> 38
<211> 189
<212> PRT
<213> 拟南芥()
<220>
<223> 转录因子shine (SHN2), AtSHN2, At5g11190
<400> 38
Met Val His Ser Arg Lys Phe Arg Gly Val Arg Gln Arg Gln Trp Gly
1 5 10 15
Ser Trp Val Ser Glu Ile Arg His Pro Leu Leu Lys Arg Arg Val Trp
20 25 30
Leu Gly Thr Phe Glu Thr Ala Glu Ala Ala Ala Arg Ala Tyr Asp Gln
35 40 45
Ala Ala Leu Leu Met Asn Gly Gln Asn Ala Lys Thr Asn Phe Pro Val
50 55 60
Val Lys Ser Glu Glu Gly Ser Asp His Val Lys Asp Val Asn Ser Pro
65 70 75 80
Leu Met Ser Pro Lys Ser Leu Ser Glu Leu Leu Asn Ala Lys Leu Arg
85 90 95
Lys Ser Cys Lys Asp Leu Thr Pro Ser Leu Thr Cys Leu Arg Leu Asp
100 105 110
Thr Asp Ser Ser His Ile Gly Val Trp Gln Lys Arg Ala Gly Ser Lys
115 120 125
Thr Ser Pro Thr Trp Val Met Arg Leu Glu Leu Gly Asn Val Val Asn
130 135 140
Glu Ser Ala Val Asp Leu Gly Leu Thr Thr Met Asn Lys Gln Asn Val
145 150 155 160
Glu Lys Glu Glu Glu Glu Glu Glu Ala Ile Ile Ser Asp Glu Asp Gln
165 170 175
Leu Ala Met Glu Met Ile Glu Glu Leu Leu Asn Trp Ser
180 185
<210> 39
<211> 189
<212> PRT
<213> 拟南芥()
<220>
<223> 转录因子shine (SHN3), AtSHN3, At5g25390
<400> 39
Met Val His Ser Lys Lys Phe Arg Gly Val Arg Gln Arg Gln Trp Gly
1 5 10 15
Ser Trp Val Ser Glu Ile Arg His Pro Leu Leu Lys Arg Arg Val Trp
20 25 30
Leu Gly Thr Phe Asp Thr Ala Glu Thr Ala Ala Arg Ala Tyr Asp Gln
35 40 45
Ala Ala Val Leu Met Asn Gly Gln Ser Ala Lys Thr Asn Phe Pro Val
50 55 60
Ile Lys Ser Asn Gly Ser Asn Ser Leu Glu Ile Asn Ser Ala Leu Arg
65 70 75 80
Ser Pro Lys Ser Leu Ser Glu Leu Leu Asn Ala Lys Leu Arg Lys Asn
85 90 95
Cys Lys Asp Gln Thr Pro Tyr Leu Thr Cys Leu Arg Leu Asp Asn Asp
100 105 110
Ser Ser His Ile Gly Val Trp Gln Lys Arg Ala Gly Ser Lys Thr Ser
115 120 125
Pro Asn Trp Val Lys Leu Val Glu Leu Gly Asp Lys Val Asn Ala Arg
130 135 140
Pro Gly Gly Asp Ile Glu Thr Asn Lys Met Lys Val Arg Asn Glu Asp
145 150 155 160
Val Gln Glu Asp Asp Gln Met Ala Met Gln Met Ile Glu Glu Leu Leu
165 170 175
Asn Trp Thr Cys Pro Gly Ser Gly Ser Ile Ala Gln Val
180 185
<210> 40
<211> 206
<212> PRT
<213> 毛果杨()
<220>
<223> 转录因子shine (SHN1), PtSHN1
<400> 40
Met Val Gln Ser Lys Lys Phe Arg Gly Val Arg Gln Arg His Trp Gly
1 5 10 15
Ser Trp Val Ser Glu Ile Arg His Pro Leu Leu Lys Arg Arg Val Trp
20 25 30
Leu Gly Thr Phe Glu Thr Ala Glu Glu Ala Ala Arg Ala Tyr Asp Gln
35 40 45
Ala Ala Ile Leu Met Ser Gly Arg Asn Ala Lys Thr Asn Phe Pro Ile
50 55 60
Pro Gln Thr Ser Asn Glu Glu Asp Pro Lys Ser Ser Asp Glu Ala Ser
65 70 75 80
Leu Pro Thr Pro Pro Asn Gly Leu Ser Glu Ile Leu His Ala Lys Leu
85 90 95
Arg Lys Cys Ser Lys Ala Pro Ser Pro Ser Met Thr Cys Leu Arg Leu
100 105 110
Asp Thr Glu Asn Ser Leu Ile Gly Val Trp Gln Lys Arg Ala Gly Glu
115 120 125
Arg Ser Asp Ser Asn Trp Val Met Arg Val Gln Leu Gly Gln Arg Glu
130 135 140
Ser Gln Val Ser Glu Ser Thr Leu Pro Leu Pro Gln Ser Ser Gly Gly
145 150 155 160
Val Ser Glu Pro Glu Leu Arg Ala Glu Met Gly Glu Asp Glu Arg Ile
165 170 175
Ala Leu Gln Met Ile Glu Glu Leu Leu Asn Arg Asn Cys Pro Ser Pro
180 185 190
Ser Phe Gly Val Gln Asp His Gly Asp Gly Ser Leu Phe Leu
195 200 205
<210> 41
<211> 208
<212> PRT
<213> 毛果杨()
<220>
<223> 转录因子shine (SHN2), PtSHN2
<400> 41
Met Val Pro Ser Lys Lys Phe Arg Gly Val Arg Gln Arg Arg Trp Gly
1 5 10 15
Ser Trp Val Ser Glu Ile Arg His Pro Leu Val Lys Arg Arg Val Trp
20 25 30
Leu Gly Thr Phe Glu Thr Ala Glu Glu Ala Ala Arg Ala Tyr Asp Gln
35 40 45
Ala Ala Ile Leu Met Ser Gly Arg Asn Ala Lys Thr Asn Phe Pro Met
50 55 60
Pro Gln Thr Ser Asn Glu Asp Asp Pro Lys Ser Ser Asp His Gln Pro
65 70 75 80
Ser Leu Thr Thr Pro Pro Asn Gly Leu Ser Gln Ile Leu His Ala Lys
85 90 95
Leu Arg Lys Cys Ser Lys Ala Pro Ser Pro Ser Met Thr Cys Leu Arg
100 105 110
Leu Asp Ala Glu Asn Ser Ile Gly Val Trp Gln Gln Arg Ala Gly Gln
115 120 125
Arg Ser Asp Ser Asn Trp Val Met Thr Val Gln Leu Gly Lys Arg Asp
130 135 140
Glu Ser Gln Val Ser Glu Ser Ala Leu Pro Leu Pro Asp Gln Ser Pro
145 150 155 160
Gly Gly Ile Ser Gly Pro Glu Trp Arg Glu Glu Met Asp Lys Glu Glu
165 170 175
Arg Val Ala Leu Gln Met Val Glu Glu Leu Leu Asn Arg Asn Cys Pro
180 185 190
Ser Pro Pro Phe Gly Val Gln Asp His Asp Asp Asp Ser Phe Phe Leu
195 200 205
<210> 42
<211> 179
<212> PRT
<213> 毛果杨()
<220>
<223> 转录因子shine (SHN3), PtSHN3
<400> 42
Met Val Gln Ser Lys Lys Phe Arg Gly Val Arg Gln Arg His Trp Gly
1 5 10 15
Ser Trp Val Ser Glu Ile Arg His Pro Leu Leu Lys Arg Arg Val Trp
20 25 30
Leu Gly Thr Phe Asp Thr Ala Glu Glu Ala Ala Arg Ala Tyr Asp Glu
35 40 45
Ala Ala Ile Leu Met Ser Gly Arg Asn Ala Lys Thr Asn Phe Pro Val
50 55 60
Val Ala Asn Gln Thr Arg Asn Gly Gln Asn Ser Pro Ser Ser Ser Ser
65 70 75 80
Ala Leu Ser Ala Lys Leu Arg Lys Tyr Cys Arg Ser Pro Tyr Pro Ser
85 90 95
Leu Thr Cys Leu Arg Leu Asp Ala Glu Asn Cys His Ile Gly Val Trp
100 105 110
Gln Lys Arg Ala Gly Pro Arg Ser Val Ser Asn Trp Ile Met Thr Val
115 120 125
Glu Leu Gly Lys Lys Asp Gly Arg Gln Ala Pro Glu Gln Lys Ile Leu
130 135 140
Ile Ser Asp Thr Ser Asp Met Ala Gly Gln Glu Gly Gly Ser Asp Asp
145 150 155 160
Gly Pro Asp Asp Glu Glu Arg Val Ala Leu Gln Met Ile Glu Glu Leu
165 170 175
Leu Asn Arg
<210> 43
<211> 178
<212> PRT
<213> 毛果杨()
<220>
<223> 转录因子shine (SHN4), PtSHN4
<400> 43
Met Val Gln Ser Lys Lys Phe Arg Gly Val Arg Gln Arg Gln Trp Gly
1 5 10 15
Ser Trp Val Ser Glu Ile Arg His Pro Leu Leu Lys Arg Arg Val Trp
20 25 30
Leu Gly Thr Phe Glu Thr Ala Glu Ala Ala Ala Arg Ala Tyr Asp Gln
35 40 45
Ala Ala Ile Leu Met Asn Gly Gln Asn Ala Lys Thr Asn Phe Pro Thr
50 55 60
Ser His Leu Asp Gln Asp Thr Asn Leu Gly Lys Asp Asn Asn Ser Pro
65 70 75 80
Leu Pro Ala Lys Ala Leu Ala Glu Leu Leu Asn Ser Lys Leu Arg Lys
85 90 95
Cys Cys Gly Lys Asp Pro Ser Pro Ser Leu Thr Cys Leu Arg Leu Asp
100 105 110
Asn Asp Asn Ser His Ile Gly Val Trp Gln Lys Lys Ala Gly Ser Arg
115 120 125
Ser Ser Ser Asn Trp Val Met Lys Val Glu Leu Gly Asn Tyr Asn Lys
130 135 140
Lys Thr Glu Ser Ser Pro Thr Val Glu Ile Glu Pro Glu Asn Gly Thr
145 150 155 160
Glu Glu Glu Asp Arg Ile Ala Met Gln Met Ile Glu Glu Leu Leu Asn
165 170 175
Arg Asn
<210> 44
<211> 192
<212> PRT
<213> 毛果杨()
<220>
<223> 转录因子shine (SHN5), PtSHN5
<400> 44
Met Val Gln Ser Lys Lys Phe Arg Gly Val Arg Gln Arg Gln Trp Gly
1 5 10 15
Ser Trp Val Ser Glu Ile Arg His Pro Leu Leu Lys Arg Arg Val Trp
20 25 30
Leu Gly Thr Phe Glu Thr Ala Glu Ala Ala Ala Arg Ala Tyr Asp Gln
35 40 45
Ala Ala Ile Leu Met Asn Gly Gln Asn Ala Lys Thr Asn Phe Pro Ala
50 55 60
Ser His Leu Asp Gln Asp Thr Lys Leu Gly Lys Asp Asn Asn Ser Pro
65 70 75 80
Leu Pro Ala Lys Ala Leu Ala Glu Leu Leu Tyr Ser Lys Leu Arg Lys
85 90 95
Cys Cys Gly Lys Asp Pro Ser Pro Ser Leu Thr Cys Leu Arg Leu Asp
100 105 110
Asn Asp Asn Ser His Ile Gly Val Trp Gln Lys Lys Ala Gly Ser Cys
115 120 125
Ser Ser Ser Asn Trp Val Met Arg Val Glu Leu Gly Asn Ser Asn Arg
130 135 140
Lys Ser Thr Gln Val Met Glu Glu Leu Arg Pro Ser Leu Ser Ser Glu
145 150 155 160
Ser Ser Ser Arg Val Glu Ile Glu Pro Glu Ile Asn Gly Thr Asp Glu
165 170 175
Glu Asp Lys Ile Ala Met Gln Met Ile Asp Glu Leu Leu Asn Cys Asn
180 185 190
<210> 45
<211> 214
<212> PRT
<213> 蒺藜状苜蓿()
<220>
<223> 转录因子shine (SHN1), MtSHN1
<400> 45
Met Val Gln Ser Lys Lys Phe Arg Gly Val Arg Gln Arg His Trp Gly
1 5 10 15
Ser Trp Val Ser Glu Ile Arg His Pro Leu Leu Lys Arg Arg Val Trp
20 25 30
Leu Gly Thr Phe Glu Thr Ala Glu Glu Ala Ala Arg Ala Tyr Asp Gln
35 40 45
Ala Ala Ile Leu Met Ser Gly Arg Asn Ala Lys Thr Asn Phe Pro Ile
50 55 60
Thr Gln Thr Ser Glu Gly Asp Pro Lys Ser Ile Thr Ser Asn Glu Asn
65 70 75 80
Lys Pro Ser Thr Ser Lys Asp Leu Glu Glu Ile Leu His Ala Lys Leu
85 90 95
Arg Lys Cys Ser Lys Val Pro Ser Pro Ser Met Thr Cys Leu Arg Leu
100 105 110
Asp Thr Glu Asn Ser His Ile Gly Val Trp Gln Lys Arg Ala Gly Lys
115 120 125
Cys Ser Glu Ser Asn Trp Val Met Thr Val Gln Leu Gly Lys Lys Met
130 135 140
Ser Val Thr Gln Asp Ser Gly Ser Ser Ser Ser Ser Val Ala Pro Ser
145 150 155 160
Ser Ala Val Ala Thr Glu Glu Glu Ile Val Arg Gly Glu Ile Asp Glu
165 170 175
Glu Asp Arg Ile Ala Leu Gln Met Ile Glu Glu Leu Leu Asn Asp Lys
180 185 190
Asn Cys Pro Ser Pro Ser Ile Asn Asn Ile Lys Gln Gly Asp Asp Ile
195 200 205
Asp Asn Ser Phe Phe Leu
210
<210> 46
<211> 175
<212> PRT
<213> 蒺藜状苜蓿()
<220>
<223> 转录因子shine (SHN2), MtSHN2
<400> 46
Met Val His Ser Lys Lys Phe Arg Gly Val Arg Gln Arg His Trp Gly
1 5 10 15
Ser Trp Val Ser Glu Ile Arg His Pro Leu Leu Lys Arg Arg Val Trp
20 25 30
Leu Gly Thr Phe Glu Thr Ala Glu Glu Ala Ala Lys Ala Tyr Asp Glu
35 40 45
Ala Ala Ile Leu Met Ser Gly Arg Asn Ala Lys Thr Asn Phe Pro Ile
50 55 60
Asn Val Glu Asn Gln Thr Asn Ser Ile Ser Ser Ser Ser Thr Ser Ser
65 70 75 80
Lys Ala Phe Ser Ala Val Leu Ser Ala Lys Leu Arg Lys Cys Cys Lys
85 90 95
Phe Pro Ser Pro Ser Leu Thr Cys Leu Arg Leu Asp Ala Glu Asn Ser
100 105 110
His Ile Gly Val Trp Gln Lys Gly Ala Gly Pro Arg Ser Glu Ser Asn
115 120 125
Trp Ile Met Met Val Glu Leu Glu Arg Lys Lys Ser Ala Ser Val Pro
130 135 140
Glu Lys Ala Lys Pro Glu Glu Leu Ser Lys Asn Gly Leu Asp Asp Glu
145 150 155 160
Gln Lys Ile Ala Leu Gln Met Ile Glu Glu Leu Leu Asn Arg Asn
165 170 175
<210> 47
<211> 196
<212> PRT
<213> 蒺藜状苜蓿()
<220>
<223> 转录因子shine (SHN3), MtSHN3
<400> 47
Met Val Lys Ser Lys Lys Phe Arg Gly Val Arg Gln Arg His Trp Gly
1 5 10 15
Ser Trp Val Ser Glu Ile Arg His Pro Leu Leu Lys Arg Arg Val Trp
20 25 30
Leu Gly Thr Phe Glu Thr Ala Glu Glu Ala Ala Arg Ala Tyr Asp Glu
35 40 45
Ala Ala Ile Leu Met Thr Asn Ser Asn Asn Lys Thr Phe Ala Thr Ser
50 55 60
Ser Ser Thr Ser Thr Lys Pro Asn Thr Ser Leu Ser Ala Ile Leu Ser
65 70 75 80
Ala Lys Leu Arg Lys Cys Cys Lys Ser Pro Ser Pro Ser Leu Thr Cys
85 90 95
Leu Arg Leu Asp Thr Glu Asn Ser His Phe Gly Val Trp Gln Lys Arg
100 105 110
Ala Gly Pro Arg Ser Asp Ser Ser Trp Ile Met Met Val Glu Leu Glu
115 120 125
Arg Lys Lys Lys Glu Gln Glu Glu Glu Ser Glu Val Leu Pro Asn Ser
130 135 140
Asp Ser Glu Thr Leu Ala Ser Val Val Asp Asn Glu Asp Ser Glu Lys
145 150 155 160
Ala Val Lys Pro Glu Asn Glu Asp Glu Glu Gly Asn Asp Lys Asn Lys
165 170 175
Gly Leu Asp Glu Glu Gln Arg Ile Ala Leu Gln Met Ile Glu Glu Leu
180 185 190
Leu Asn Arg Asn
195
<210> 48
<211> 208
<212> PRT
<213> 蒺藜状苜蓿()
<220>
<223> 转录因子shine (SHN4), MtSHN4
<400> 48
Met Val Gln Gln Thr Lys Lys Phe Arg Gly Val Arg Gln Arg Gln Trp
1 5 10 15
Gly Ser Trp Val Ser Glu Ile Arg His Pro Leu Leu Lys Arg Arg Val
20 25 30
Trp Leu Gly Thr Phe Glu Thr Ala Glu Ala Ala Ala Arg Ala Tyr Asp
35 40 45
Gln Ala Ala Ile Leu Met Asn Gly Gln Ser Ala Lys Thr Asn Phe Pro
50 55 60
Val Thr Lys Asn Gln Gly Glu Glu Val Ala Ser Asp Thr Pro Tyr Asn
65 70 75 80
Gly Gly Gly Gly Asp Asp Ser Phe Leu Ser Pro Lys Ala Leu Ser Glu
85 90 95
Leu Leu Ser Thr Lys Leu Arg Lys Tyr Cys Lys Asp Pro Ser Pro Ser
100 105 110
Leu Thr Cys Leu Arg Leu Asp Asn Asp Asn Ser His Ile Gly Val Trp
115 120 125
Gln Lys Arg Ala Gly Pro His Ser Asp Ser Asn Trp Val Met Arg Val
130 135 140
Glu Leu Gly Gly Lys Lys Lys Thr Ile Glu Ser Glu Glu Ile Gly Ser
145 150 155 160
Lys Gln His Thr Ile Asp Gly Gly Asn Asn Ser Asn Ala Asp Asn Glu
165 170 175
Asn Arg Val Val Val Glu Glu Glu Glu Arg Val Ala Leu Gln Met Ile
180 185 190
Glu Glu Leu Leu Asn Trp Asn Tyr Pro Cys Gly Ser Thr Ser Ser Asn
195 200 205
<210> 49
<211> 197
<212> PRT
<213> 蒺藜状苜蓿()
<220>
<223> 转录因子shine (SHN5), MtSHN5
<400> 49
Met Val Gln Arg Asn Lys Phe Arg Gly Val Arg Gln Arg Gln Trp Gly
1 5 10 15
Ser Trp Val Ser Glu Ile Arg His Pro Leu Leu Lys Arg Arg Val Trp
20 25 30
Leu Gly Thr Phe Glu Thr Ala Glu Ala Ala Ala Arg Ala Tyr Asp Gln
35 40 45
Ala Ala Ile Leu Met Asn Gly Lys Asn Ala Lys Thr Asn Phe Pro Ile
50 55 60
Pro Lys Asp Gln Thr Glu Asp Ala Asn Ser Leu Thr Pro Asn Cys Asp
65 70 75 80
Asp Asn Asn Asn Ser Phe His Thr Ser Asn Ala Leu Ser His Leu Leu
85 90 95
Lys Gln Lys Leu Thr Lys Cys Cys Gln Lys Gln Ser Gln Ser Leu Thr
100 105 110
Cys Leu Arg Leu Asp Ala Asp Asn Ser His Ile Gly Val Trp Gln Lys
115 120 125
Gly Ala Gly Ser His Ser Asp Ser Asn Trp Ile Leu Arg Val Glu Leu
130 135 140
Gly Lys Lys His Glu Asp Ser His Glu Ser Asn Tyr Val Ser Ser Ser
145 150 155 160
Glu Lys Ser Ala Pro Asn Asn Ser Thr Ile Val Gly Asp Cys Ala Glu
165 170 175
Lys Asn Gly Ile Glu His Glu Glu Asp Ile Val Thr Met Gln Met Ile
180 185 190
Glu Glu Leu Leu Asn
195
<210> 50
<211> 205
<212> PRT
<213> 水稻()
<220>
<223> 转录因子shine (SHN1), OsSHN1
<400> 50
Met Val Gln Pro Lys Lys Lys Phe Arg Gly Val Arg Gln Arg His Trp
1 5 10 15
Gly Ser Trp Val Ser Glu Ile Arg His Pro Leu Leu Lys Arg Arg Val
20 25 30
Trp Leu Gly Thr Phe Glu Thr Ala Glu Glu Ala Ala Arg Ala Tyr Asp
35 40 45
Glu Ala Ala Val Leu Met Ser Gly Arg Asn Ala Lys Thr Asn Phe Pro
50 55 60
Val Gln Arg Asn Ser Thr Gly Asp Leu Ala Thr Ala Ala Asp Gln Asp
65 70 75 80
Ala Arg Ser Asn Gly Gly Ser Arg Asn Ser Ser Ala Gly Asn Leu Ser
85 90 95
Gln Ile Leu Ser Ala Lys Leu Arg Lys Cys Cys Lys Ala Pro Ser Pro
100 105 110
Ser Leu Thr Cys Leu Arg Leu Asp Pro Glu Lys Ser His Ile Gly Val
115 120 125
Trp Gln Lys Arg Ala Gly Ala Arg Ala Asp Ser Asn Trp Val Met Thr
130 135 140
Val Glu Leu Asn Lys Glu Val Glu Pro Thr Glu Pro Ala Ala Gln Pro
145 150 155 160
Thr Ser Thr Ala Thr Ala Ser Gln Val Thr Met Asp Asp Glu Glu Lys
165 170 175
Ile Ala Leu Gln Met Ile Glu Glu Leu Leu Ser Arg Ser Ser Pro Ala
180 185 190
Ser Pro Ser His Gly Glu Gly Glu Gly Ser Phe Val Ile
195 200 205
<210> 51
<211> 225
<212> PRT
<213> 紫短柄草()
<220>
<223> 转录因子shine (SHN1), BdSHN1
<400> 51
Met Val Gln Ser Lys Lys Lys Phe Arg Gly Val Arg Gln Arg His Trp
1 5 10 15
Gly Ser Trp Val Ser Glu Ile Arg His Pro Leu Leu Lys Arg Arg Val
20 25 30
Trp Leu Gly Thr Phe Glu Thr Ala Glu Glu Ala Ala Arg Ala Tyr Asp
35 40 45
Glu Ala Ala Ile Leu Met Ser Gly Arg Asn Ala Lys Thr Asn Phe Pro
50 55 60
Val Pro Arg Ser Ala Thr Gly Glu Ile Ile Val Ala Pro Ala Ala Ala
65 70 75 80
Arg Asp Ser Arg Gly Gly Gly Leu Gly Ser Ser Ser Gly Ala Gly Ser
85 90 95
Leu Ser Gln Ile Leu Ser Ala Lys Leu Arg Lys Cys Cys Lys Thr Pro
100 105 110
Ser Pro Ser Leu Thr Cys Leu Arg Leu Asp Thr Glu Lys Ser His Ile
115 120 125
Gly Val Trp Gln Lys Arg Ala Gly Thr Arg Ala Asp Ser Ser Trp Val
130 135 140
Met Thr Val Glu Leu Asn Lys Glu Pro Ala Ala Ala Ala Thr Thr Thr
145 150 155 160
Thr Leu Ser Asp Ser Val Ala Pro Thr Thr Pro Ser Thr Ser Ser Thr
165 170 175
Ser Ala Ser Thr Ala Gly Ser Pro Pro Val Gly Met Asp Asp Glu Glu
180 185 190
Arg Ile Ala Leu Gln Met Ile Glu Glu Leu Leu Gly Gly Ser Ser Pro
195 200 205
Asn Ser Pro Ser His Gly Leu Leu Gln Gly Glu Glu Gly Ser Phe Val
210 215 220
Ile
225
<210> 52
<211> 211
<212> PRT
<213> 紫短柄草()
<220>
<223> 转录因子shine (SHN2), BdSHN2
<400> 52
Met Val Gln Pro Lys Lys Lys Phe Arg Gly Val Arg Gln Arg His Trp
1 5 10 15
Gly Ser Trp Val Ser Glu Ile Arg His Pro Leu Leu Lys Arg Arg Val
20 25 30
Trp Leu Gly Thr Phe Glu Thr Ala Glu Glu Ala Ala Arg Ala Tyr Asp
35 40 45
Glu Ala Ala Val Leu Met Ser Gly Arg Asn Ala Lys Thr Asn Phe Pro
50 55 60
Val Gln Arg Ser Ser Thr Gly Asp Pro Ala Pro Ala Ala Gly Arg Asp
65 70 75 80
Val Arg Gly Gly Asn Gly Gly Gly Ser Ser Ser Ser Ser Met Ser Asn
85 90 95
Leu Ser Gln Ile Leu Ser Ala Lys Leu Arg Lys Cys Cys Lys Ala Pro
100 105 110
Ser Pro Ser Leu Thr Cys Leu Arg Leu Asp Pro Glu Lys Ser His Ile
115 120 125
Gly Val Trp Gln Lys Arg Ala Gly Ala Arg Ala Asp Ser Asn Trp Val
130 135 140
Met Thr Val Glu Leu Asn Lys Gly Val Gly Leu Pro Ser Asp Val Glu
145 150 155 160
Ala Gln Ser Thr Ile Ser Thr Ala Thr Thr Ser Ser Ser Val Ser Thr
165 170 175
Met Asp Asp Glu Glu Lys Leu Thr Leu Gln Met Ile Glu Glu Leu Leu
180 185 190
Ser Arg Ser Gly Pro Val Ser Pro Ser His Gly Glu Asp Glu Gly Asp
195 200 205
Phe Val Val
210
<210> 53
<211> 204
<212> PRT
<213> 玉米()
<220>
<223> 转录因子shine (SHN1), ZmSHN1
<400> 53
Met Val Gln Pro Lys Lys Phe Arg Gly Val Arg Gln Arg His Trp Gly
1 5 10 15
Ser Trp Val Ser Glu Ile Arg His Pro Leu Leu Lys Arg Arg Val Trp
20 25 30
Leu Gly Thr Phe Glu Thr Ala Glu Glu Ala Ala Arg Ala Tyr Asp Glu
35 40 45
Ala Ala Val Leu Met Ser Gly Arg Asn Ala Lys Thr Asn Phe Pro Ile
50 55 60
Gln Arg Ser Ser Thr Gly Glu Pro Thr Pro Ala Ala Gly Arg Asp Ala
65 70 75 80
Arg Ser Asn Phe Ser Ser Gly Ser Ser Thr Thr Asn Leu Ser Gln Ile
85 90 95
Leu Ser Ala Lys Leu Arg Lys Cys Cys Lys Ala Pro Ser Pro Ser Leu
100 105 110
Thr Cys Leu Arg Leu Asp Pro Glu Lys Ser His Ile Gly Val Trp Gln
115 120 125
Lys Arg Ala Gly Ala Arg Ala Asp Ser Asn Trp Val Met Thr Val Glu
130 135 140
Leu Asn Lys Asp Ala Ala Ser Thr Asp Ala Ala Ser Gln Ser Thr Ser
145 150 155 160
Ala Thr Thr Ala Pro Pro Ala Thr Pro Met Asp Glu Glu Glu Arg Ile
165 170 175
Ala Leu Gln Met Ile Glu Glu Leu Leu Ser Ser Ser Ser Pro Ala Ser
180 185 190
Pro Ser Asn Gly Asp Asp Gln Gly Arg Phe Ile Ile
195 200
<210> 54
<211> 204
<212> PRT
<213> 两色高梁()
<220>
<223> 转录因子shine (SHN1), SbSHN1
<400> 54
Met Val Gln Pro Lys Lys Phe Arg Gly Val Arg Gln Arg His Trp Gly
1 5 10 15
Ser Trp Val Ser Glu Ile Arg His Pro Leu Leu Lys Arg Arg Val Trp
20 25 30
Leu Gly Thr Phe Glu Thr Ala Glu Glu Ala Ala Arg Ala Tyr Asp Glu
35 40 45
Ala Ala Val Leu Met Ser Gly Arg Asn Ala Lys Thr Asn Phe Pro Val
50 55 60
Gln Arg Ser Ser Thr Gly Glu Pro Thr Pro Ala Ala Gly Arg Asp Ala
65 70 75 80
His Ser Asn Ala Gly Ser Gly Ser Ser Thr Ala Asn Leu Ser Gln Ile
85 90 95
Leu Ser Ala Lys Leu Arg Lys Cys Cys Lys Ala Pro Ser Pro Ser Leu
100 105 110
Thr Cys Leu Arg Leu Asp Pro Glu Lys Ser His Ile Gly Val Trp Gln
115 120 125
Lys Arg Ala Gly Ala Arg Ala Asp Ser Asn Trp Val Met Thr Val Glu
130 135 140
Leu Asn Lys Gly Ala Ala Ser Thr Asp Ala Ala Ser Gln Ser Thr Ser
145 150 155 160
Ala Thr Thr Ala Pro Pro Ala Thr Pro Met Asp Asp Glu Glu Arg Ile
165 170 175
Ala Leu Gln Met Ile Glu Glu Leu Leu Ser Ser Ser Ser Pro Ala Ser
180 185 190
Pro Ser His Gly Asp Asp Gln Gly Arg Phe Ile Ile
195 200
<210> 55
<211> 235
<212> PRT
<213> 两色高梁()
<220>
<223> 转录因子shine (SHN2), SbSHN2
<400> 55
Met Val Gln Ser Lys Lys Lys Phe Arg Gly Val Arg Gln Arg His Trp
1 5 10 15
Gly Ser Trp Val Ser Glu Ile Arg His Pro Leu Leu Lys Arg Arg Val
20 25 30
Trp Leu Gly Thr Phe Glu Thr Ala Glu Glu Ala Ala Arg Ala Tyr Asp
35 40 45
Glu Ala Ala Val Leu Met Ser Gly Arg Asn Ala Lys Thr Asn Phe Pro
50 55 60
Val Pro Arg Thr Ala Thr Gly Glu Leu Ala Pro Val Pro Ala Ala Arg
65 70 75 80
Asp Ala Arg Gly Gly Gly Gly Ser Ser Ser Ala Ala Ala Ala Pro Gly
85 90 95
Gly Gly Thr Ser Asn Leu Ser Gln Ile Leu Ser Ala Lys Leu Arg Lys
100 105 110
Cys Cys Lys Thr Pro Ser Pro Ser Leu Thr Cys Leu Arg Leu Asp Pro
115 120 125
Glu Lys Ser His Ile Gly Val Trp Gln Lys Arg Ala Gly Ala Arg Ala
130 135 140
Asp Ser Ser Trp Val Met Thr Val Gln Leu Asn Lys Asp Val Pro Pro
145 150 155 160
Pro Ala Ser Ser Ser Gly Glu Glu Pro Val Pro Ser Asp Gly Gly Ala
165 170 175
Ala Ala Thr Thr Pro Thr Ser Thr Ser Thr Ser Ser Thr Val Thr Thr
180 185 190
Thr Gly Ser Pro Pro Pro Ala Met Met Met Asp Asp Glu Glu Arg Ile
195 200 205
Ala Leu Gln Met Ile Glu Glu Leu Leu Gly Ser Ser His Ser His Gly
210 215 220
Met Phe Gln Gly Ala Ala Gly Ser Ile Val Ile
225 230 235
<210> 56
<211> 171
<212> PRT
<213> 大麦()
<220>
<223> 转录因子shine (SHN1), HvSHN1
<400> 56
Met Val Gln Ser Lys Lys Lys Phe Arg Gly Val Arg Gln Arg His Trp
1 5 10 15
Gly Ser Trp Val Ser Glu Ile Arg His Pro Leu Leu Lys Arg Arg Val
20 25 30
Trp Leu Gly Thr Phe Glu Thr Ala Glu Glu Ala Ala Arg Ala Tyr Asp
35 40 45
Glu Ala Ala Ile Leu Met Ser Gly Arg Asn Ala Lys Thr Asn Phe Pro
50 55 60
Val Pro Arg Ser Ala Asn Gly Glu Ile Ile Val Ala Pro Ala Ala Ala
65 70 75 80
Ala Arg Asp Ile Arg Gly Gly Val Gly Ser Ser Ser Ser Gly Ala Ala
85 90 95
Gly Ala Ser Ser Leu Ser Gln Ile Leu Ser Ala Lys Leu Arg Lys Cys
100 105 110
Cys Lys Thr Pro Ser Pro Ser Leu Thr Cys Leu Arg Leu Asp Thr Glu
115 120 125
Lys Ser His Ile Gly Val Trp Gln Lys Arg Ala Gly Ala Arg Ala Asp
130 135 140
Ser Ser Trp Val Met Thr Val Glu Leu Asn Lys Glu Pro Ala Ala Ala
145 150 155 160
Ala Pro Pro Thr Pro Ser Asp Ser Thr Val Ser
165 170
<210> 57
<211> 209
<212> PRT
<213> 西加云杉()
<220>
<223> 转录因子shine (SHN1), PsSHN1
<400> 57
Met Ala Arg Pro Gln Arg Tyr Arg Gly Val Arg Gln Arg His Trp Gly
1 5 10 15
Ser Trp Val Ser Glu Ile Arg His Pro Leu Leu Lys Thr Arg Ile Trp
20 25 30
Leu Gly Thr Phe Glu Thr Ala Glu Asp Ala Ala Arg Ala Tyr Asp Glu
35 40 45
Ala Ala Arg Met Met Cys Gly Pro Arg Ala Arg Thr Asn Phe Pro Phe
50 55 60
Asn Pro Asn Ala Pro Gln Ser Pro Ser Ser Lys Val Leu Ser Ser Thr
65 70 75 80
Leu Thr Ala Lys Leu His Arg Cys Tyr Met Ala Ser Met Gln Gly Pro
85 90 95
Arg Ser Gly Ser Ser Lys Lys Asp Ser Met Ala Arg Ala Asp Lys Asn
100 105 110
Asn Asn Ile His Ser Gly Asn Gln Ser Leu Thr Cys Leu Arg Leu Asp
115 120 125
Asn Glu Arg Ser Asn Asn Ile Gly Ile Trp Gln Lys Lys Ser Gly Ser
130 135 140
Lys Gln Ser Glu Ser Asn Trp Leu Met Lys Leu Glu Leu Asp His Asp
145 150 155 160
Gln His Gly Asn Ser Thr Leu Lys Arg Glu Thr Asp Asp Asp Ile Ala
165 170 175
Gln Met Ile Glu Glu Leu Leu Asp Cys Gly Ser Leu Glu Ile Cys Ser
180 185 190
Pro Ile Ala Ser Ala Asp Ser Asn Ile Asn Ser Ala Glu Ser Met Leu
195 200 205
Asn
<210> 58
<211> 224
<212> PRT
<213> 江南卷柏()
<220>
<223> 转录因子shine (SHN1), SmSHN1
<400> 58
Met Gly Arg Pro Gln Arg Tyr Arg Gly Val Arg Gln Arg His Trp Gly
1 5 10 15
Ser Trp Val Ser Glu Ile Arg His Pro Leu Leu Lys Thr Arg Val Trp
20 25 30
Leu Gly Thr Phe Glu Thr Ala Glu Asp Ala Ala Arg Ala Tyr Asp Glu
35 40 45
Ala Ala Arg Leu Met Gly Gly Pro Arg Ala Arg Thr Asn Phe Pro Tyr
50 55 60
Asp Pro Asn Ala Pro Pro His Pro Ser Ser Ser Thr Leu Leu Ser Thr
65 70 75 80
Leu Ser Ala Lys Leu Asn Arg Cys Phe Ser Ser Ser Ser Ser Ser Ser
85 90 95
Ser Cys Ser Thr Asp Pro His Lys Lys Asp Pro Arg Val Ser Gln Ser
100 105 110
Leu Thr Cys Leu Arg Leu Asp Pro Glu Gln Ser Asn Leu Gly Ile Trp
115 120 125
Gln Lys Lys Ser Gly Arg Gln Pro Glu Ser Asn Trp Val Met Lys Val
130 135 140
His Phe Gly Ser Gln Gly Gly Gly Gly Val Ser Ser Asp Ile Val Leu
145 150 155 160
Pro Thr Asp Asn Pro Ala Pro Pro Gln Pro Ile Glu His Lys Lys Met
165 170 175
Lys Ser Glu Glu Asp Leu Ala Thr Glu Met Ile Glu Glu Leu Leu Asn
180 185 190
Phe Pro Asp Ser Ser Ser Pro Ser Ser Ser Thr Ser Ser Ser Glu Ala
195 200 205
Lys Asn Pro Asn Phe Ser Ser Ser Asp Leu Leu His His Ile Leu Val
210 215 220
<210> 59
<211> 212
<212> PRT
<213> 小立碗藓()
<220>
<223> 转录因子shine (SHN1), PpSHN1
<400> 59
Met Gly Arg Pro Gln Arg Tyr Arg Gly Val Arg Gln Arg His Trp Gly
1 5 10 15
Ser Trp Val Ser Glu Ile Arg His Pro Leu Leu Lys Thr Arg Val Trp
20 25 30
Leu Gly Thr Phe Glu Thr Ala Glu Asp Ala Ala His Ala Tyr Asp Glu
35 40 45
Ala Ala Arg Leu Met Cys Gly Val Arg Ala Arg Thr Asn Phe Pro Tyr
50 55 60
Asp Pro Asn Ala Ser Lys Arg Pro Asn Ser Gln Met Leu Ser Ala Thr
65 70 75 80
Leu Ser Ala Lys Leu His Arg Trp Tyr Leu His Ser Gln Gln Arg Asp
85 90 95
Gly Gln Glu Gly Lys Ser Lys Asp Ala Arg Met Thr Gln Ser Leu Thr
100 105 110
Cys Leu Cys Leu Asp Ala Glu Gln Ser Asn Leu Gly Ile Trp Gln Lys
115 120 125
Lys Thr Gly Arg Gln Ala Glu Ala Asn Trp Val Arg Lys Val Gln Phe
130 135 140
Gly Asp Asn Asn Ser Pro Gln Thr Asp Ser Pro Gln Pro Glu Asn Ser
145 150 155 160
Ser Glu Ser Cys Met Ser Glu Glu Asp Lys Phe Ala Ala Glu Met Ile
165 170 175
Glu Glu Leu Leu Gly Tyr Ser Pro Gly Gln Phe Ser Asn Phe Gly Ser
180 185 190
Pro Ala Met Ser Asp Ser Ser Cys Ser Ser Ser Cys Ser Ala Val Thr
195 200 205
Thr Ala Phe Glu
210
<210> 60
<211> 3000
<212> DNA
<213> 拟南芥()
<220>
<221> 启动子
<222> (1)..(.3000)
<223> 醛脱羰酶(CER1)蜡/角质启动子, pAtCER1, At1g02205
<400> 60
tgggatcctc tccatcgttt ccatcatggg atacattact tacattgact tcatgaacaa 60
catgggacat tgcaacttcg agcttttccc taagcgtctc ttccacctct tccctcccct 120
caagttcctc tgttacaccc cctcgtacgt cgtttgtaaa aacactacta tttatttgga 180
tttgaatata tgcatgcatt tataacatgg catcaaatat gttgaaggtt tcactcgctc 240
caccacacgc agttccggac caactactct ctgttcatgc caatatacga cttcatctac 300
gggacgactg ataatttgac cgactcacta tacgagaggt cgttggagat agaggaagaa 360
tcacccgacg tcatccacct cactcacctc accacacaca actccatcta ccaaatgcgc 420
cttggtttcc cgtccctctc ctcttgcccc ttgtggtctc gacccccatg gtacctcaca 480
tgcttcatgt ggcccttcac tctcctctgc tcatttgccc tcacttcagc tatccctttg 540
cgcacctttg tctttgagcg aaaccgtctc cgtgacctca ccgttcactc tcacctcctt 600
cccaagtttt cctttcacgt aagcatttta cacgcatgca tatgcacttt ccctctctct 660
cgctctcttg acacaaacgg gtttacttat tatgttgtac ttcacaccac tattttccaa 720
actttcacac acaaaaaaaa cattgattct cacttaactt gtacgtacta cgtaggaagg 780
acataacctt ttatacaaaa agaaaattaa aacaacctaa aaatgaacaa tgaacatttt 840
cttgtgggta taccttttgt aagttgcatc taaagtttat taataatttt gcctcataaa 900
catgcggcgg tagtataaat cacagcgcca ccatgagtcc atcaacacta tcatagagga 960
agctatcctt gaagcagatg aaaagggtgt gaaagtaatg agtcttggcc tgatgaacaa 1020
tgtaagttta tttacttcaa atccccctaa atatatatga atcactattg gataagtact 1080
gtaactaatt acacacgctg gcaaaagaaa aaagtaacta atcaacacac aattaattaa 1140
tgagagggag gaactaaacg ggtctggaga aatgtacgtg caaaagtatc cgaagttgaa 1200
gataagacta gtggacggga gcagcatggc agctacggtg gttatcaaca atattccaaa 1260
ggaagccacg gaaattgtct ttagaggaaa tctcacaaag gtggcttcgg ctgttgtctt 1320
tgctctgtgc caaaagggcg tcaaggtttg tgaatatatg agggaattgg aatatatata 1380
ctcagcttct catatcaaac aaaaaataat gtagtaatgt gtatatatag gtggtcgtgt 1440
tacgcgagga ggaacacagc aaactcatca aatctggggt tgacaagaat ctggtactgt 1500
ctacaagcaa tagttattac tccccaaagg tgtggttggt gggggatgga atagagaacg 1560
aagagcagat gaaagcaaaa gaaggaaccc tctttgttcc cttttctcac tttccgccca 1620
acaaactccg caaggactgt ttctaccagt ccactccagc tatgcgtgtt cccaagtctg 1680
cccaaaacat cgactcctgt gaggtacatc tttgaattct tatagatata tctgtaactt 1740
ttatattata taagctgata gatgtgttca tctataatga atgaatggtt gttatatata 1800
tatagaactg gctggggagg agggtgatga gtgcatggaa aataggaggt atagtgcatg 1860
cacttgaggg ttgggaggag catgactgcg gcaacacttg caacgtcctc cgtctccacg 1920
ccatatggga agctgctctt cgccatgatt tccaacctct cccaccatct cctctatgag 1980
cttttttcat attcatacat ctatgtcccc tttcttgatt atatctactt cccttccatc 2040
attgtttgct gtttactatg tttttctatc gacaatatat aagtaccctt gttacccttg 2100
gtgccacgtg cttcatatat gttagaaagg gcaaaaaaat tcgtcgtatg atatgcttag 2160
ttaaatttta taaaactcaa taaaaatctt cagaaacagt gctatgatca ttacatctta 2220
actaagtgat atatatctgc gtgcctattt aacaaaataa acaaaaaaac aaaacaaaat 2280
atatttgggt gcatcatcaa atcaaagtag ttgcaaaaac tggacgaggt ttttacttaa 2340
atggtcctta ccccgcatgg tccacttgct acctaattaa ggattggtag ggtgcgtata 2400
cgtatataaa ttgtggcggt gggagatgga gttactaaaa acgaaacgta caagtattat 2460
tcatagctct cgtataaggg gttagtcctt agatctagat attttcactt ttctttcatt 2520
tatgtcggag caacagacac tagctggcgc ttcaacgtgc atgatcttga ttggctagta 2580
aattccaagc atcaatacct aacacatgcc caacttggtt cattagtatt ctttcattgg 2640
taaaataccc ttacctttca ataatatcca gaaataaata tatgaagcca tccatcaacc 2700
ggtgcatttc ctcaaggcat ggatatgata tcagaacatc gatgaaggtg ggagggggta 2760
attagctgag tgtcataaat gaggatccat gtggagatca tcgaatggta gtagtacatg 2820
tttggtctta gctggcccca ccacaaggaa ttggactggt gggaagatag gggtggttac 2880
gtcattccac atatctacca attaaggagt ttaatataaa ccttgctata taatgtacct 2940
tggctcacaa gagttgaaga gacacagtga cgacacaaac atattacatt cgacggtata 3000
<210> 61
<211> 3000
<212> DNA
<213> 拟南芥()
<220>
<221> 启动子
<222> (1)..(.3000)
<223> BAHD-型酰基-转移酶(CER2, VC2),蜡/角质启动子, pAtCER2, At4g24510
<400> 61
atcgtactaa aaatctggga agtacactga tccgatggaa atgaaggaga agattgtaca 60
ttttatcgtg atgattcgtc ataaactgag actggagaga gcgaagctga acagctttag 120
cagctgaagc tgcttcctct tctggcttag aagcattcct tggacgaccg tgcattcttc 180
tccgacgact gtgagagatg cgacagtgtg attcaacttt tccggttaaa tctgatcaga 240
aattacgaaa acagttaaaa caaacactag atcgaggaaa gaaaaaacgt gatccaaatc 300
tagaaacaac agagatgacg aaatggagaa taagaagcag atcacctgaa aggagcacgt 360
tgcggcgtcg atcgagagag agagactttt gttttcgggc aattgagcga ctgagagaag 420
gttctatcgg aaaatttaat gggccggttt aatgatttca atcgcttaaa ccagtaaacc 480
gggtttaatt gaaaaatacg gtcgaacact aacccggttt tggaagtctt cgtttatgta 540
gtgaaaggct tcgtcatcat cgttgtttca cagttgaagg ttttcacgag aaagtgaaga 600
tttttggaga aaccatactt ttgtgaaggt tcgtttcacc ttcaatttcg tttttccaat 660
ttgggtatcc gataattgta agaatttggg gttttaaggt cagattgatt atgtatctaa 720
tgcttttaca ttgtagtagc tcgtgttttg gattttgaag gattgatttc taaatttcct 780
tttgatcttg tagttgaatc acaatggaag attcagagaa aagaaaacag atgttaaaag 840
caatgcgaat ggaagctgca gcgcagaatg atgatgatgc tactacaggt actgaaacat 900
ctatgagcac aggtcacctc tccaatccat tggcagagac atccaatcac cagcaggatt 960
catttgaaac gcaaaggttt gattattata ccgatcccat ggctgcttat tctagtttca 1020
agaaaaacaa gacccctaag caacaataca tctcatctcc tagtcatcaa ggaagctctc 1080
ctgtaccacc tcagtttcca ccatcagttc ctccaggtgc ttgtttctgc cttgatagtc 1140
aatgactctg tcttaatttt tgttttgtct atattcttgt gaggccaaag aaagtagtcc 1200
actgataaag aagattgcct aatatcatga gagtttaact tacagtatgt tagttggtcc 1260
ttggtgagta taggtatagg attgaacaga atcaggaatt cactttggta tttggttgtt 1320
gggctttcgt agagttaggt gatgattctc ttctacaaat ctatatgaaa tttttgttct 1380
ttgtttgggt ttagtcatgt gttcttgatt ataatctaca aatcaagatc aatggaggaa 1440
gaaatttgtc tctcagtctc aggcttcaaa cttattagct ttagctactt taatttgata 1500
tatctcttta agtttcactt tctaggaatt gacagtatga aaatcacatc aattatggaa 1560
ttcagttagg tttgtgtcct gtgccttgca atgaacagta atccagttct gatccttgca 1620
ttataacttt gaacaggatc attatgtagt gagtatcagg cacagaccaa tcatggtggc 1680
tttcatgcag ctcattatga accaagaggg atggcacatc tttcaccctc acacagaggt 1740
ccacccgccg gttggaataa caactttagg cctccaccag ttaaccattc aggtccacct 1800
caatgggtac ctcgcccttt tccattctct caagaaatgc cgaacatggg gaataataga 1860
tttggtggtc gaggcagcta caacaatact cctccacagt tttccaatta tggacgacaa 1920
aatgcaaact ggggtggaaa cacgtatcct aactcaggaa gaggcagaag tcgaggacgc 1980
ggtatgaaca caagctttgg gagagatgga ggaagaagac ccatggaacc aggggcagaa 2040
cgattttact ccaactctat ggctgaagat ccatggaagc atcttaagcc agtcttatgg 2100
aagaattgct cagatgcttc gagcagcagc tcaacaggtc aagcctggct tcccaagtct 2160
atagcaccaa aaaaatctgt gacctcagaa gctacccaca aaactagcag taatcagcag 2220
agccttgctg agtaccttgc tgcttctcta gatggtgcta catgcgatga gtcaagcaat 2280
taatcaggac aactggccta gtgaaaatac accaagtcca agcatctgag gtacctggaa 2340
agatcaccgt tgactctttc gaactaggta ttgttcagtg acttgtgttt attttcttaa 2400
caagacacaa cagcgaatga tgaacatctc tgagggcgca attaggagta gattggttgg 2460
caatagggat gttctctacc aaaaatttta ctgttttttc gcaagattta gttatcgtac 2520
aattatgtaa aatcattatc aggaaatttg ttgcatgatt gtgtttgagg tggaaatgaa 2580
ccgcatccgt attaagatca tttttgctgg tggaaacaat gttaccagga aactgaactt 2640
ggttttttat agattaatgt gacttgttag gtaccgtaat ataatactag ttggctacga 2700
cacgtacatg tgcgtttatt gcttgaagcc aataaggaca aggtggacgt aataaagtgt 2760
gcttgttgtt ggatggatct gaatatgatg actcaactgt ccaactctaa tgttgttgct 2820
aaagacccaa atcccaccca catttaatgt tgccgtcacg gaaacagttt tcccaactgt 2880
cctaaatcag tgatacccat gcctattctg aactcaactc tctttcgaaa ctcaatcctt 2940
atataacaca tcccatttaa gcctataagc tacacatatc agctctctca caaaaataaa 3000
<210> 62
<211> 3000
<212> DNA
<213> 拟南芥()
<220>
<221> 启动子
<222> (1)..(.3000)
<223> 甾醇去饱和酶(CER3, WAX2),蜡/角质启动子, pAtCER3, At5g57800
<400> 62
attttaagaa attaaatctt tttttttggt atctgtgtgt tactttttat aaatgtattt 60
tgaaaagtat tatttcaatt tatagataaa tataattatg actctcaagt ggattttttt 120
ttcacggtca attggagttt ggctgtgaat cttgtgaata atatcaacta attaagagtc 180
ggccgatact ccgtatcttt gatcactttc acccatatct tgaaatttac caaatgtttt 240
ctttcattgg ggtttctata cctttttttt ttggttcttt ctgtaatttg ggttttaaag 300
aaaaaaccta taattacaaa aaaggttaaa agttaaaact aagcacgttc aaatatgttc 360
tttcgagatc ccaccttcct gtctatctta taagaagtcg aacacgtgtg ctcgttgtgt 420
gaattagagt ggttatttca acatatataa ttaaactatg tatgtatcat aaatacactg 480
aatatgttgg agttgggtct tgtggtaggc taacttgttc gcttcacttt ggtttagttt 540
agccttgtaa tcggtggttt catatactga aatttttaat tttctaatat aatccagatg 600
acaaaaaaaa atcataaata cactgcaatg gccattttta aaaaaacttt aaacgaaata 660
tatatacatc accaagtatc aagtaagtaa tttggttgtt ttgatttttc aaaaaaacat 720
ttaggtgatt cttttaacaa ccaatcatat atgtagcaaa gattaattat gtcaatcttt 780
taacgtcact tatatagttt agctgtagta ttattgtaag ccaattgtac ggacttcgta 840
accgtttcga caaagataca aactaatcaa tgtttaagat ggcataatat tccgacttcc 900
cctgccactc ggtagtactg ctgcgccatc ctacaagaag cactcgtaaa tgagtggtta 960
gacggccaaa aattagcagg attcaatcct tgattaagag atggttcaga taagccctct 1020
aggcctctac tcgtcacaat tcatatatta attgatcaca ctgcatgaaa tgataagatc 1080
aagtcaatta cctcaaaatt aattgaaaga taaaatgata aagcaacaag taatgatcat 1140
atatggatgt aacaaccttc ttcatccata cgaatatgat aattcttttg tcgatcattt 1200
gccacatcta tttaactaag tattttgtac attaagatca ttttgcgagt attcccgcgt 1260
tcccaacaaa aacgaattga aaaactacat agggtgaatt ttgctctcgt ggcattaaag 1320
agagaggctg acattataga ttatgatata tttattattt ttcttagtag atttcttgac 1380
cagcctttag atatgcttgc tctgtcatgg cataaagcct taaaccctaa agccctttac 1440
atttgtacag taccaacaat tgtacccaat gttaaatgaa tgcggattaa tttgtgatat 1500
gagaccatca aaataaaata cttataagat agtagatata tagaaccgta acaaaaagat 1560
gagaagaaaa agacaaattg aatagaaact ggaccaaaag ataaaagagt atatgaagaa 1620
gcaagccacc aagaagtgta accatcgttg cttcaactgt aaaccgcaca ttaaatgtgt 1680
gtgagagaaa gagagataga ctccagctgt gagagctacg tccattgcat caattgggtt 1740
atatgtcgtt aacaattttt taaaaataca taaataaatc aataaaatat ttagaaaaaa 1800
tcaatattat aatgatattt tgaagaagtt aggtccataa tggaaatata gagacttagt 1860
gtctatttat caagaaatag tgtctattta tcaagaaata atgtctattt ggtccacaat 1920
tatcatattt tttgtggcca ctcaaactac gaaaataatg aatattgtta atttttgtct 1980
tatttaagtc tataactaac ttactaattc ggttgaaatt gctaatatgc attttccttg 2040
gtccactatt acagtgctac tgctgtctta ttcttgaaca caaaataaaa aagatttcaa 2100
acacaaaata tgcatttttt tgttccacca ttacaaacct ttatttggag aagtatacag 2160
ttggatatta aatattttaa ttaaaaaatt gaaaatattg aagttctttt tctccagtat 2220
tttggctgga gcaaaactaa ataaattcca aattaaaagc tcgaacggaa aacatttact 2280
atgcaccaaa ataaatgttg atatgaaatt tgcatcagca tatacagtat gtatgtagta 2340
caattgaaca gagtcaaaat taattgtcac tgaaacagtc agattttatt tctgttaaaa 2400
aatattttca gacaaaaaat gattttttaa cgttttttta tataatttta ttaaacctca 2460
aaacattcct cagcagattg ttttataatg ttattattat tttttcttaa ataattttat 2520
ttgaatttcg ttatagagag ataaataaaa gatttttggg gtctagttgg ctctcatagt 2580
ttgaatgctt atataaggaa acagagctcg aggctctcaa taatgccaca taaaacacaa 2640
caccaaagcc tttataactc tttctggggt ttttaattct ctcaacgaac aaaaaaaaaa 2700
actcaactaa tattctccat cctctttctt cagatttaag agaaacctta tcgttatatt 2760
ctctcttctc ttttcttttt gactcccaca agaagaaagc aaatttcttt ttctcaggat 2820
tgcttgaaaa ctgtagaaat ctttaaggtt tttttacacc aataagagtc agagagagag 2880
agagagagag aaagagagat ctattcataa aaaagacagg taatctcaac tccgaggaga 2940
taactgatat aacaatttga gagaataaga agaagaagaa gaagaagacc aagctaaaga 3000
<210> 63
<211> 3000
<212> DNA
<213> 拟南芥()
<220>
<221> 启动子
<222> (1)..(.3000)
<223> 脂肪酰基辅酶A还原酶(CER4, FAR3),蜡/角质启动子, pAtCER4, At4g33790
<400> 63
ttcttcaagg aacaaagatc tatcttttgg ttgcgtttac atagtgttta cgttccatgt 60
tataattatt agctcatatt agaaacccag acgtaaacac aaacaaataa tcatgtcact 120
atacagaatt tacctagtat agaagtccat gatttttgtt ctttttacct tttaatagaa 180
aagttttgtt ctttagtttt ttgggattta acattcgttt taatatatct tgtagatgtt 240
gcagaaaagt gttggtggta ataagataga gcaaaccttt ccagaaagta cattggatta 300
ctcacaaggc ttctcggcta ctcagtttca ggtttctctc tcttcactat ctctctgtta 360
ggttctgttg agtacacgtg acttgtctca agaatctcac tatatatgtc tttgggtgtt 420
gaaacaagca ggacaaaaca gcattccaag agcagtgtgg ttatttgcat atggaaacca 480
gattctaaat aaccagttca gtaatttttt attattattt ctgtagatgt tcaactactt 540
tgtttattct tactttataa agaatatatc aaatctgact aaaattaact ttgaaattcg 600
ctggtgaaga tcaaactgat gaagagcagc agctattttg gacatttgtg aagcttttat 660
ggattcaact tgaatcattt caagcagtga ttaaagtgaa actttaacgt cccaaatatg 720
aaaaatagga aggagtttta agcaaaagac tgatatagaa cgaaaaaaaa aaagggttct 780
caaatctcaa ctggactgat tcttttctct cttttgcttt gtaatttcgt cacaaacaag 840
ttttccttgt agccgccttt atgtaactcg tggtttacaa attacaacac ttcacttcca 900
ttgcaatttt ctttcatagt ataacaaatt tcaatgcctg aataagcttt ttttttgtgt 960
ggggaaaaaa acttccaaga aatatctagc aaaacaaatg atcttaatgg ttcttttcag 1020
agctgctaaa ttctcaaaag aataattcga agtagatgac aaagagggca tattcaaaac 1080
ttataaagaa actaataaaa cgtattactt taaatagagc acgaaaacct atatataacc 1140
aaaatgctaa ccctaaaagt aacacttgaa gaatgcgtaa cactaacgca ataaaagggg 1200
aaaatagaaa cttatcaaaa tctggttatc ggcttgtttt tatagtcact cttcatatgc 1260
atcagtgttt gagattctgc agattgttgt agcagatcat gtttaaagtc cgttatttgt 1320
agctcttttg agttactagc gattttggag gtgttaaccg gcccaagcat gaataggcct 1380
gatggtattc tggtccatct gagcttggcc gaagttaatt gcaaaattaa aattaagttt 1440
atattattat gcttttgtct atctcttgac tttaattaat cttttaaggt taattggtta 1500
ttggtagttg agggtgtttc agttaacctt aaataaaaaa cttaattgac ttaaattata 1560
gtaaaaacca aaccaaacca aatagagatc caaatttaaa tgtacataat ctaccacttt 1620
cataatgtaa aaatccaagt gttatcagct gccgcatttg ttctgtcttc tatatgtcga 1680
atacgagtac ataaactaca actttgataa tctaatttta ggattttaat atgctaggct 1740
gttttgcgtt tgtcatgagt agggtcgaat catgccttag ccagtcgtac tagtatagat 1800
ggttgtaagt tggatttgat gacaactgct ttggatttga tgacaagtaa ctacttaaaa 1860
gcaatggtta ggcagacatt aaataggtgg tgatctaatg tctaacatct aaaacgtttt 1920
aattggaatt taaacgagtt tagatggtct aattaagcgt ttattatgta cataatagaa 1980
cgtataaaat atcatgattc acagaagtaa atttaaaatg ttttaatttt agaagtaaat 2040
ttaaaattca aactacatca aaaattcaat agaaatcaac ttaaagataa ccataaaatc 2100
aattcaattg acatcaaata atagaagcca tctacttttt atgttataaa acttataatg 2160
aattttatgt cattataaat cgaaaaacta cactgaaatg aacaaaaagt caaatgacaa 2220
cagattaatg attatgtaaa ctgtataatg tcgattaaaa ccacataatg ccatcaatta 2280
tttagcacct atcaaataat tgtcacggtt gtatatatgg cgcgcctaga ccgccactta 2340
aagcgctatt tcaagtattg ttacaaacga agaaggtagg cggtctaatt tttaagtcta 2400
aaattagata tctttggacg cctcatatta cttattgttt tactctgtcg gatcctatct 2460
cacccaacca tacatttgtg gcaacagaaa ttgcctccaa atgcaaaaaa aaaaatcatt 2520
ctttctactg ttaatacttc gaaatataca atttttaaag ttaatatgtg tttaaatacc 2580
attttgatct aaattttgtg ttttgtgttt ctttctatat atatatgtac gttttccatt 2640
atatcagaat cgaatcatat ctaaccatat atgaattagt ctcggtagtt gtaatttgga 2700
atatttattt cgcttttcaa cgttggtagg ttcaaaaata cttaagttaa gccacgttta 2760
atttaaatac gttcatattt agaatatgta gagttgaaat ttataactta agtatatgtt 2820
atggaatttg ttgacagcta ttttaaagta gttgggacaa aacaaaaatg taggtaggtg 2880
ctcacattaa tttcaccttg taaagcctaa aactataaat cttagtaaca catatgtgat 2940
atgtcagttg tactctgttg cattatttct ctcactcaaa cgtatataca aacataaatt 3000
<210> 64
<211> 3000
<212> DNA
<213> 拟南芥()
<220>
<221> 启动子
<222> (1)..(.3000)
<223> ABC转运蛋白(CER5, WBC12, ABCG12),蜡/角质启动子, pAtCER5, At1g51500
<400> 64
tattgtgaag ctaatttttt tgcttagtta tgttgatatc attttgcctt ttggtttgtt 60
atagtgaagc ttgatttgtt ggattgttgt catgatgtaa tttagagatg aatgcaatac 120
tgttttgtcg tagatgttga ctttactctt tgacatgctt tgtgttaatc aatgtaatac 180
tgaatatctg aaattgagag ccaaaaatga taaatttatc tttgcaatgt tacattgctt 240
ttaagaggga gatgtaatat tctgtaaagt gcaatgctgt tccctaagga cacttcgttt 300
aagttacttc aaagtgtcag aaatgacggg tcgaataaga taagctgtct tctagatgcg 360
agtttaggaa tcaaatatta tttttcttta gtttgcttga gttctcatgg aagtggaagt 420
aacactttgc ttttgttttt tttgataagg gatatgcttt gatagaatat gagaagaagg 480
aagaagcaca gagcgctatt tcagcaatga atggtgctga gcttctaaca cagaatgtta 540
gcgttgactg ggccttcagc agcggtccca gtggtggtga atcttacagg agaaagaact 600
cgaggtttgt atcctaatct cttttctgtt ccgctacgac ttgtctgatc ttgtgccgat 660
atgaagtcaa gagtgtcaaa aagtatcaat taagatatag acttagaagt tttaacagtt 720
gacaaggtag tatattgtac taagcaccta ccaaacttta ctgtctttat gctaatgaat 780
cgaaatcact tgtgacttgt caagtagtta tacctttctt taaatcatca tccattgatc 840
attggggttt tacaggtatg ggaggtcgca acgttcaaga agtccgagaa gacgttactg 900
attgtatgat ttggtggaga gttctcccca tatctctctg tattagggat tccctctctt 960
atctgtaagg tactgatgca agacgctgag tgttgaacaa atttttttct ggggtttttg 1020
tagggtttgg ctgcttttgc attggtttat ctctttatgc atttgcatgg gattgttgcg 1080
tttaaacacc atgagatctg ttttaatgct ttgtattggt aaaagaagag aaacattgga 1140
tgaggaagaa tgttttaatt ttcattctct tattgattca tatttaggtc agacagaatt 1200
tgaaaatcaa agaccttcca ttacactaga aaaccaaata tgattatata gaaaaaatca 1260
aagaattttc aaccaatggc attatttcac actggtttat tattctaggc gtaactattg 1320
gtaacctaaa tatgttaaca gaaatcatca aatgactatt aaatattttg gctgaaattt 1380
tccaatcaat ggcaatattc acatcgtttg tttttatagg cgtaactatt cgtatcctaa 1440
atatgttaaa agaaatcatc aaaggtttga tgatttgaga tagagcatgt actttcttgc 1500
tactcacagg ttgcatgatt tcatttgtac gaatcaatca tctatacttt gtgcattgag 1560
ccagtcccac attatgttta tgtatccatc tattttaagg gctctcatat actaacttac 1620
agctgaaaag tataaatatg aaacagttgg aacagttata taagttctgc ttttgttgtc 1680
accaacaaaa aaaatgatat gcttttacat gattccaaca aaaaaaaaac agttttgtat 1740
tcttgttaat gatggtgcca aaggcttata agaccatacc ttagattctg ttgaatttag 1800
aatctgggtt tctatctctt ttctggaaat cttgtttctt tttgttttct ttttatacca 1860
aaactttgtg aaatcttagg tacttttgtt tagtatcagt ttactcgttt aaaaagaacc 1920
aataaaccta ctagatataa atagtaagaa gtctataact tagggttgtg ttaaatacta 1980
aacaatgctt aaaaatgtgg ttagacatag cgctatacta aagctaaaaa gctaaagata 2040
accataaaat catgaagata ataaatggac ggatagacca aaaagaaata atgaagcata 2100
atacaaaaaa aaaactatat cggttccaac taccacacat ttaattcctt tcccttttca 2160
tttaacacct gtgaagactc gttgcgaggg ttctctacct ctttatgtca ttcaaactta 2220
tcaattatca agtctcttga ttttacatgt tcaaccaaac acgatttttc aaacttaatt 2280
ttatatatct tcttcattaa ttatcgaata tcacttctct tgcttatagt tacaaaaact 2340
aatacaatga taattatata attctatgtg tgaacaaaac gataaatttg tcaaagtatc 2400
actatcaatc ccttggaatt agtagacaac caaatatgga tcctttaatt gatttgtgat 2460
gtttctattc atttcgaact aaatgaaaac atctcatcgt aaataatcac actgttaaat 2520
ttcatttcac aaaaatatca tttgatttct gtgtggacga aatttcttgt tagttggcaa 2580
gaaccgacat aaaaaatata tttcatacgt ataatagtat gtcaataagt aggtggttgc 2640
ttactagtta ctactacaca tggacgattg tataaatatt agacctcatt cacacaactc 2700
tcactctctt acatttaatg tgcaagaaaa taaaaaaaac tatatactat agacgtgtat 2760
ctatatatgg ttatgatatt aaagccatcc attaaatgta attagttgtt tgttgattgt 2820
tgctctcaag tctcacctaa agaaaacctt aggcctacaa aaattaatca ctatctttat 2880
aattccatct ccaaccaata taataaaaat aacctagacg aagaacaaac aaaactctct 2940
ctttctctct ctctctctaa taagtaaggt tttgatcttt ttcaagatca aacaaaaaca 3000
<210> 65
<211> 3000
<212> DNA
<213> 拟南芥()
<220>
<221> 启动子
<222> (1)..(.3000)
<223> 极长链脂肪酸缩合酶 (CER6, CUT1, KCS6),蜡/角质启动子, pAtCER6,At1g68530
<400> 65
actaaaaatt aagggaactt gtctttgata ttttatttac tagttatata tagtccttac 60
cgaacctcgt cggcaacatc atcagtgcga tcaagaatcg atcaccatta aaatgcttta 120
ttgagcacga tcagtgttca ccatgtaaga agattattgt ctcacaattc aaatcaaacc 180
tagcctaggc aacaaaaatt aaatttcttt aaacaataga acaaaactcg tccgccccaa 240
ccatcctctt cacaacattc cttaatctta caatatctat ataactaacg catcatttgg 300
atttcactat gtaactaagt cgaactggtg tgaaataatt ataaatttaa ataaataaaa 360
aggtgaaaat atggttgtac acgaaggtat gtgaaataat tatgaataac taaaatgtac 420
tgaattattt attttatttt aatagcaatg gaatctaaat taaatcagga tataatgaaa 480
attttgtaaa attgtgatta aattgtcatt ccaaagaaat cagagaactg tctttaaaaa 540
tttggaagcg agtttaatga tatagcctat atgaatggta cagtgtgaat gtctctgaga 600
aaaattacaa ttccacattt ttattacaga ctagatgaga aaaaaacatt gatgagtagg 660
tcaaattaaa aaactatatt cacgttttcg gtgatacatt ggaaaaccat ataaaacgat 720
catttttcaa aggaagaata gaaactttat ttacatattt aattattatg gaagatgaag 780
gaagaacttt atcatagact acgaaatcgg tgtggctaat gaaatacaga caccttatat 840
attacatatt aaatgtacat ttcaatctgg tggatagttc caaactaatc ttcttcatca 900
aatattgttg gcattaatga tctcactatg gaacacgaaa attcagcaat gttcattttt 960
agttaagaaa caaatcctct tacgaactct gtaagatctt gaaggatcaa aagcctttga 1020
tctcaatttc tgtatagttt ttattagcct agtgctttat atatgtttga tacttctgtt 1080
tggcaatatc aatcatagta gaaaagatat ggacttcatt tgaggttttt ggtggattgt 1140
gtctatatgt gaaatcatgg gatctcaaga tttgtctgca ttcagtttcc aagtcaaaca 1200
tcgtaactac tgtttgattt tccctcatgc ttgcagtttt catggatatc tcaagatttg 1260
tcttcttgca ctttccaagt caaacataaa gtaactactg attgatattc cctcgtgtat 1320
taccctcttt caaatgacac aattgggccc aagtagagga atttcatagt gaattcaaaa 1380
gattaactgt attccaccgt cgtattttga taacatttag ttattccttt tctttttttt 1440
cttctgcaac agtttttttt taatacattt agtgttggtt tggttcaatg aaatattata 1500
tgttacttct ttttttggaa ataaattatt cattctttct actataaaag gaattgttca 1560
tgcttttttg atacaatagt ataccatttc aaaagatacc atagaccagt tattacatga 1620
atcgccaaaa caacactaaa atcagaaaat cagtatattt tggtatagtc tccaacatac 1680
aatcataaaa cctctgtgaa atttaaaatc tatatttgac atttcaaagt ttaacaacat 1740
agttctaaat aattacctaa attttaagtc aaatgtgaat tatattttac tcttcgatat 1800
cggttgttga cgattaacca tgcaaaaaag aaacattaat tgcgaatgta aataacaaaa 1860
catgtaactc ttgtagatat acatgtatcg acatttaaac ccgaatatat atgtatacct 1920
ataatttctc tgattttcac gctacctgcc acgtacatgg gtgataggtc caaactcaca 1980
agtaaaagtt tacgtacagt gaattcgtct ttttgggtat aaacgtacat ttaatttaca 2040
cgtaagaaag gattaccaat tctttcattt atggtaccag acagagttaa ggcaaacaag 2100
agaaacatat agagttttga tatgttttct tggataaata ttaaattgat gcaatattta 2160
gggatggaca caaggtaata tatgcctttt aaggtatatg tgctatatga atcgtttcgc 2220
atgggtacta aaattatttg tccttacttt atataaacaa attccaacaa aatcaagttt 2280
ttgctaaaac tagtttattt gcgggttatt taattaccta tcatattact tgtaatatca 2340
ttcgtatgtt aacgggtaaa ccaaaccaaa ccggatattg aactattaaa aatcttgtaa 2400
atttgacaca aactaatgaa tatctaaatt atgttactgc tatgataacg accatttttg 2460
tttttgagaa ccataatata aattacaggt acgtgacaag tactaagtat ttatatccac 2520
ctttagtcac agtaccaata ttgcgcctac cgggcaacgt gaacgtgatc atcaaatcaa 2580
agtagttacc aaacgctttg atctcgataa aactaaaagc tgacacgtct tgctgtttct 2640
taatttattt ctcttacaac gacaattttg agaaatatga aatttttata tcgaaaggga 2700
acagtcctta tcatttgctc ccatcacttg cttttgtcta gttacaactg gaaatcgaag 2760
agaagtatta caaaaacatt tttctcgtca tttataaaaa aatgacaaaa aattaaatag 2820
agagcaaagc aagagcgttg ggtgacgttg gtctcttcat taactcctct catctacccc 2880
ttcctctgtt cgcctttata tccttcacct tccctctctc atcttcatta actcatcttc 2940
aaaaataccc taatcacatt ttgtaacaat aatacaatta tacattaaaa ctctccgacg 3000
<210> 66
<211> 3000
<212> DNA
<213> 拟南芥()
<220>
<221> 启动子
<222> (1)..(.3000)
<223> 烯酰辅酶A还原酶(CER10, ECR),蜡/角质启动子, pAtCER10, At3g55360
<400> 66
cctttgcatg gcgagatttt ttatcccgaa agttcagtct aagaatgatg taaaattcct 60
ctgttaagaa aaaaatgcct ttaacaaaat aaagaattcg atgttgaaga agaagaatat 120
atatattttg gtgctaattt gcagcacaag atataaaaat tagaaagtat aagccaaaaa 180
atcaaatcat gattcgacaa ggttgttttt aattctttcg tgattcttga tgttataccc 240
ctaatataac ttaccgataa cattgaatta gtgaaaaata tttcgaatta tgatcaccag 300
tctctatatt atatttgatg attttatggt tacgaatgtg aggggtacgt aagattagta 360
aagacggaca tgttgtagga aggtgtccca gtccgcgatt tctaaaagaa agatttgtgg 420
tccaaacgta aaatttaatc agaccttctt tttaatggac ctcactatgt cgacacacaa 480
catcaaacat atccctaact atatagttca taatcaagat gttttttacc agaaaaatat 540
aacatcaaac atgtctctct ctaatcatta tatttataag tttttcgtat cagactaaga 600
aagtatatat caacctgaga caaaatgatc cggtaagaag aacttcaatt tagatgacaa 660
aacttccaat taatttgaat tacttagaaa gggatgaagt tgagacacaa agaaagaata 720
taatagatat attgccttag cttgtaattc acagtttagt tgtttcaaag agcaatatca 780
ttggaatttc gtagtaacca atatatattg tggttatata tttgtgggga gtgtgtatat 840
gtatttgata tgttgatatt tttcttcaaa agacagatga tagagctaaa acgatttttt 900
gtcgatatgt tgcgcttcga agtagctgaa cctgagtgat aatgtcttca tctattggac 960
aagtagggaa gaagctagct cgccgtatgc aaatacataa agttccatgt ttcacttctt 1020
ctaggggtga atagacgagg acacacgata cagtttgtca tgttaatctt tcaagctcaa 1080
aaaatataaa aattgtatga aaatctgata cggtaaatct atatattctt ttgcatgttt 1140
ttaaaggagc acttatcttt atttgttata gaaatttatt atttaaaaga aattcgtctt 1200
tatagaaatg tgcatgtgtt ttttttatta gtaacacatt ttcatgcaac aggaatcttc 1260
gcttatctgt agtgtacatc tcatgagccc atattcatag ttgtaaagca acaccttctc 1320
tgcagctttt tctttcctat tgttttttgt atccatagtt cacatctaca cactatataa 1380
ggctattaca aacaatgata caatatatgt tctcatataa tttgatctta attttcaaga 1440
tatttaaggt gggaaaatat cgagatagta tacaatggaa atcgttacca cgtgcatgcc 1500
aatctttagt agaagaagat tcctagattc caacaagaac atgcgcattt tttcacgaaa 1560
ggctggaaag tttaatatat aatggtcgaa aaaataggca taatctattt aagtactagc 1620
ttaagtgttt tacgctagat ctggtgttga cacttgtaca cctggtttta aagttgcaga 1680
gaaaactaga ttaatgctca aattgtagta agtagattgg ataatttaca ctcggaaaaa 1740
acaagttttt tcagaagaat tagactgttg ctcacttgag aaaaatctag ctagctttgt 1800
taattgagga tagttagtta tagtatttga atgacattaa ggtgtttgaa tatcgcatga 1860
attgtcgcct tggtatttgg gcaattaccg gtcattattt tgatctttgt gatgtcaact 1920
aatcgatccc aaccatatat atcataaact actgtctctt aattgatcca ttgtgttttt 1980
aattttatca ttactttttt taactagttg agagaatgat tatctaaccc ctttcatatt 2040
cagaaaactt aggttttgag tttttgcttt atttgctgct ctcttgaatt aatagagctc 2100
tcgtatgcga ttgactttgg gtgatttttt tttgttggaa aagcaaattt cattgaccaa 2160
tataactaaa ctaacacact acttccatgt ggttaagggc cgggatcatt tattatttgc 2220
atcttttata cgacaatgac aaggattcgt tctcattact ccatcccatt ttgtggtatg 2280
taaaatccta acttttttac tgcattgata caacatttta ttttagcatt agatcaatac 2340
gatggacata tgtcatcaag tgagggggta gaagtttgca actaatgttt ttgtgctcat 2400
gaatcaatct cttctaggta acattatttc actcttttct atagcgaata ctgcaatgct 2460
tttacgtttt aactaaacat gactaaacca aataccaaaa aaaaaacgag taacactttg 2520
gtggttccta tctaagttga ccggatcact cgatttgata aaaaaaatta tagtaaaagt 2580
ttttgttttt gaatacaaat tatatatttg actaaaattc gaaacttaac cgaaactaaa 2640
accttagcag ataatctata gtatagtaga ttcataaacc gaaaaaacaa aaatctaaac 2700
ctaaactgag taattactaa tggtggatcc ttaaaacaaa gccataattt gcaattgcaa 2760
caaatgtttt tgtttggagt aaaaaccaaa aaaaaaatga tggggagcaa ctaaaaatgt 2820
aaaatggtcc caatctaatc gcataagttg ggaagatagt tagtagttct cctactccca 2880
actacatcct cttcaatgct caatctcgtc ttcttcttcc tctctattta atttcccttg 2940
tctctctatc tctctcaatt tcctcatctg ggtcttcctc gtttgctccg cttaagcacc 3000
<210> 67
<211> 3000
<212> DNA
<213> 拟南芥()
<220>
<221> 启动子
<222> (1)..(.3000)
<223> 蜡酯合酶(WSD1),蜡/角质启动子, pAtWSD1, At5g37300
<400> 67
atcacaaatg atcagttcca gtgtcattag acttgagcgt tagggagttt ttaagaggac 60
aaaatctatt ctgtcttccc gaaaaccact atgaccgtga gggggaaaca acttcgggaa 120
gatggttaaa gggacatacc gttcatcggt ggtctaatcg atctttttct cgcaggttat 180
tctcgagttc tcgcacacat aaaagttttt aatcgtatga aagttcccga aaatttgaca 240
aaaacttaat tttttacaat tataaaattt catttaaaaa aaaacttgtt tataatttgc 300
tttcttccta ttaatccttt gattcctaga cttattttgc tttcttgctc ctaaattaat 360
tgagttgctc tcttggttcg atttaaattg tttgatcgac atcgatctgg agtgaatttg 420
gaggaagtgt agaccctaaa aggtcgtatc tcaaactcga tgttggcatg tgatgcaaga 480
atttgactta caagtgggtg tagatcaagg gttgatgtta atctactatc cgatgtaatt 540
cgatacgttc cattgatgtg aggaggggcc aatgttcatg cttaagcctc tagtgttatc 600
accgaactca agacaattga atgctgcaag attgtgtact aagagtatgc agactgattt 660
tacgtgtcaa gactttccac tgcgatttca accacagtgt acgtatgttt tcagtgacaa 720
taatacggta ttattaatac ccgactcttt cacaaacttt ggaaattagt ttgagttcaa 780
ttcaaaatcg catttaaaaa aataataaaa aaattacatg aaacccacaa tatttggtat 840
gaaatttaat atggtagtta gtaacctcgt aggtacaaca ttcatcttct tgctgccgac 900
atcataattg ttcataaata tttttgaaca cgtacatata atgttcatgg accaaatctg 960
atcagactcc taattagatg aattgtgtgg tcgattttga gatgtacttt ttgaatcata 1020
attctttcaa ctactcgaaa taaatttccc tcaccatccc aagactacta gaatagcata 1080
ttcttaaata taaattatta tataaccaaa ctattattct ttttaagatt atcactggat 1140
gttacgttga agttataaga catgatatat atcactcaat ttcagaaatg agtagagttc 1200
ttagtgcata gtttttctta aacctacaag gctacaatga ataaagcacg acaccatact 1260
gttctcaaaa tatttgtata tagtggaact cggttgaaac atatatatac cagtaaagtt 1320
atttttgttt ttgttaataa aagagtaact cgtgaacttg aaatcaagga gctaataaaa 1380
aattattttt gtttgaattt aactttttca aaacctaaca cgaaaatcag aaattctgag 1440
gggttagaat ggttctcact tgtttacgtt tctacgtcca aaatactcat tgagttttca 1500
cattctcaag ttttggaaaa attaacaatt tcgtttatac cactaacatg tttaaaacta 1560
taattattct aaaactttaa ttatcaagtt catttcgttt agctaaaatt tattctgtta 1620
tatagttatt tattggatat aaaccctaaa tttagtgttt tgatgccaca cgtaagctga 1680
ttttcaaatc acaactttat aaattccctc attaatttat cttctaatgc atctatcaac 1740
aataacgtta tttatatatg ttgtctatat ctattaatct aaatttgtta caaacaattt 1800
aagtcagcac ttatcggttt tatgtctaca tcatacatta tgtaggttgt acgatgacgt 1860
gggttgtagc tagttaggta taaaaattta cttttaaaag aaaaacataa tctcctcatc 1920
aaaagctttt aattgaattt aatagccagg gccaaatatg ccaacaatat acaccaaaaa 1980
acatatttgt cgctagaaca gtgctaatta agggactcac atactattag acattagtgc 2040
atgtcagcca ataggcttat tgtattttca acatgttggc ccatgtcagc caataggctt 2100
atagtatttg ctaaaaataa aaatatatat atttgctcaa gtgttggtgt tgcatggttc 2160
aatatttatc aaaactcaaa agaattgttg actaacttta catattaaca agactaatca 2220
tttttctggt ttgtccttaa ttttaaatat gtaaaccact catttatgat ttctccaatt 2280
tagaaagagt gagatgtgaa gtttctccca aagaaagaaa aaaactacag gtaaataaag 2340
tgtatactgc tttccaaaat atgtcgaatt atcacaaaaa taaaacgaat ttataattaa 2400
acatctcgtt atggattttg tatagaaatt tattggccta caactgttaa tagacatatt 2460
atttggcatt gtacgtaatc tagattagcc aaactatata tagaacatca tgtttagctg 2520
atttttcaaa aaaaaaaaaa aaaaaatcat gcgtagacga aatgcaagtt ttaatttaat 2580
tttattgctt tctctggtta gctctagagg tctttaatcc accgatctta aaaaaaaatc 2640
caactttcta ctttctcttt ctaaatgaaa caattccata tttttcattt catttataca 2700
tcatacactt aattaatgat attctctttc tagtaattag tacattaatt taaatttatc 2760
aagaaaatgc tgttgaacat atataatcaa ttattatgaa atgaaattta taatgaaatg 2820
atttgtttga tagaaagaat aaattataaa ctacttaaga gaccgacgaa gatagcaacg 2880
acaaaaccct aaccttatac caaccctcat ttaataccaa taaaaaaaga gggacaaaaa 2940
aaatcaatgc tatataatcg aagtatccac tacaaaggtt ggagaatcct taaactgact 3000
<210> 68
<211> 3000
<212> DNA
<213> 拟南芥()
<220>
<221> 启动子
<222> (1)..(.3000)
<223> 中链烷烃水解酶(MAH1, CYP96A15),蜡/角质启动子, pAtMAH1, At1g57750
<400> 68
gtacaaaaaa aagaacaaaa tctatcatca tttaattcgt tgctttattt aaataaaaaa 60
cacaaaaact tgaaatcata tactttatat gataaattta aaatctatat tagtagtata 120
aatttaaaat aatttaaggg acggtatacc acgagtggga tcctaaaatg attggattta 180
ttatgataat aatttatttt tttgaatttt ataacactaa taatttagct aacgagataa 240
tatatacgga tttatatggg atgacgggtt ttgatacata ttgataaaga aaagtttatc 300
acggatgaaa tcttaaaatt aacaatcaaa atacaattat acaaccttaa acacaaaaaa 360
aaatattcaa tattaacaaa acaaatataa cttaaaattt aaaaacttta gctataaaaa 420
atcggtccac aatatattgc ggataaaata ttcaaaattt taaatgtata aaaatatatc 480
ttttgtatta atacttcaaa aagtgaagcc taaacaatag taattatata ctaaataata 540
aagaaataga tatctattta aaatacaaat taatatgata tatttttagt taatctttaa 600
aaaaaaatat tacatttaaa aaacaatatt acattttaaa gaaaaatata gtcccgcggt 660
ataccgcggg ttaatttcta gttttagatt ataactgaag ttgttactta caaaacaaat 720
acagatttaa aaaaaaaaaa ctaattatcc agacgaaaca aagaggatca taattattag 780
tacaaaaatc taagatataa acagtagtag ataaatatta cccactcata gtataaacag 840
tagaggataa cttttggcat tgatggacgt aataaaaacc aaaatgttgt catactaacc 900
aattaactac taactagaaa ccagactagc caggctgtag ggaagaacaa cattgattga 960
tgtagtgcta catatcgatt aactactaac tagaaaccag actaaccagt ccatcattaa 1020
tatgtataaa tacatattgt atatacatat ttttaacaaa tgataagata atagtagtat 1080
tagttttttt tggtgagtaa tagactaata gtattagttt ttacaacagt ttaaattgtt 1140
caaacaaaat aaaacgatgt agtctatata aataagcaaa acaaggttcc cggcggtata 1200
atatacgcat ggtcgatcaa tgaaaattat ggatctaatc agtaaaacat tattatttct 1260
tacgtaatta caatagtaat ttatctcaaa ggtaaaaaat tattataaat aagtaaaata 1320
caaaatctaa atagcaatga aaagaagaaa aaaaaaacat atttctcata aaacaattta 1380
cgtagttaag aagatttttt tttttcacaa aaaagtagtt aagaagattt tatctttcat 1440
ttaattgtag tggaaagatg ttttttctcg cataacatgt catttttatg tgagtggcta 1500
atttattaaa ttggttagat tttagtagtt tagatttagg ggttagtttt gttaaaaaat 1560
tatatgataa attagagggc cacaaaaaac atattttgaa atttaatacg tcatgactct 1620
taaaacagta aaatttaata cgtcatgact cttaaattta gccgttggat gatgaatatg 1680
cacgactttt gtgatgtaca ttattaacaa gttatgaact aattagcaat gacaatatac 1740
gtcataatat acggtataaa ataattcaga catattttac ctaacaatat agcgatgaat 1800
gatgtatgtg gtaacttatt gacaaattta atatgtcggt aaatataatc agtcactaat 1860
ataacaacgg atatgcgatg ataataggtt ctaggtcttt agcaatgaga taagcgagat 1920
atttatgtaa cattagtctt taaattttat atatatgtct tttctatttt attttatttt 1980
ttgaatgaaa atgtcttctt tattcgtaat tttaaactca ctggtggtgg atatattgtt 2040
atgtccccaa ttcgtctggc aactctcgta tattagtgag aaaaatttgt ccattattta 2100
ctgcactatt accctgtgtt aattttttgt attgaaattg ttttttagta attcacgtca 2160
tatagcgaat gattctttaa ttttaaaaat tcagtcttaa gtttacaaat taaataacgc 2220
tactgtaacc aactctgtac gaccaacatg ttcgagtttt tgtatatacg gccatatatg 2280
tacatatttt actataaagc gaaaaaatcc ataaattatt taattaatat ataaaggtgc 2340
cattctattt ccaatgtgct taggaaaatg cagaacctcg tgctatatct ctgtcgccac 2400
gtgcaaatat aacaatatga aatagaacta gcaaatcttg aaatctaact cttaagacta 2460
attcaagcac atacgtagag aaagttgacc aacggttatc agcattttaa catggacctt 2520
atcaacattt taacaaagtc cacaaacaac cagtcttaca atcgcattgg tacaagataa 2580
tcgaattcat cttccatata acaaaaccta aaccttggtg tgaaaaggag aagatatgta 2640
tgttaaaggc cgcctatgcc tctggtttgg ggtatatgat tctaagatta gggtttgaat 2700
attttcgtta gcctgccatg agatatattt atgtgataat tagagcctct tatgcattaa 2760
tgcataaccg actagatcat gtggtattca gctaatcagt acacacaaga caaagtagta 2820
aatgagtttg atgaagactg tggtctgata attcctatca acgttaaatc tgtcggggcc 2880
aggcagccag caacattttg cctaacaacg ctctgaattc aattgaacct aggctatata 2940
atagcaggct aacttaacta agagttttgt atctatactc ataaatcctt ttgtctaaaa 3000
<210> 69
<211> 3000
<212> DNA
<213> 拟南芥()
<220>
<221> 启动子
<222> (1)..(.3000)
<223> ABC转运蛋白(WBC11, ABCG11, DSO, COF1),蜡/角质启动子, pAtWBC11,At1g17840
<400> 69
gatgcgacgg atacaaataa acgtatcatt tcacttttct cgtctttgcg gtggtttaac 60
ttttgtttgt gtgataacta atctatcata ttgaatgatt ttaagaacga aaaggtaagt 120
aaaagctatt tttgaaaaat acagtagtat gtattcgttt cttcttcctc agtctgtaag 180
taaacacgga ttcaatagtg aaaagaaaaa agaaaacacg gattcaatta ttgagcacta 240
attggttaaa ggcccattat gttccatttt gttttttggg ctttgactct tttgtcacac 300
atgcgttgac gtagtagaat tttataaaca ttttagccca atataacatt ctttcagcat 360
cttgatcctt cctgtatgat aaaattaaaa atcgccatga atctttggaa atcaacaaag 420
ggccagaact tataagaata tttgttttga ttattaaggt ctataaatgt ctgtcaaaca 480
aaaatgcatg tattgtctat atcatcaaag tatgaatatc caatactgtt aaaatattta 540
tttatccgca taaatacaaa tacctctgaa tatagttttt atttggatta aatactagaa 600
tttaattaag aattaattgt ctaaataacc ctaaaaaata aaataaaatt acaaacaggc 660
atttacgtaa cgtaatgact tacagaaaca agcactttta tctttgcaaa ataacggatt 720
taccttcatt catccacaat attacaataa tagctgagac ttttgacgac gactatggtg 780
aactccggcc atagaaactc aaatgacggt gtgataaacg aaaaatcgtg aaagacatga 840
ctcgaatctg aaacagttgt gtaaggatct ctctgtcacc gttggcatat aaataccatt 900
gtttctctcc tttagattcc aaatttctgt tttaatcaca ctctctgttg ttgcagttta 960
gatctatttc tgaaatctca atagaaatct ttcccgattc gtttgtatgt gatctggttt 1020
ttgtaaatag ccattatcgg gagaaattat gttgaaatca atttctcctt tgtcacgtcg 1080
agatcaactt ctctgatatt ttccttcatc atttcctatt acatctcaca actctaagaa 1140
tatcttctct gcaatctcaa tgtcaatcac aattcatcaa ttccaaacac aaatcttttt 1200
cttctcctaa ctatcacaaa attagatgag tgtttatgat tagaaccctt gggttccata 1260
tatactatct cattagggtt tagattttgc ttgtattctc ttaattttgt ttgattaaaa 1320
tcgatttcaa ggtttctgta aataaataaa tacaaattga agaagaagaa cgatggagaa 1380
gaagcatgaa caatgaaaca ggggcaaatc gtaagttggc atatctgaaa gtttcttttt 1440
tcaaatccca tctcaaaaat ggttaccgag aggaagcaaa tgtttttggt cgacggagta 1500
acgtggtggt ggtggtcgag aaagaagaaa gaaggagatg atacagatga caggggtaaa 1560
acagtctttt tccctagcac aaagtactct ttttcaaaat ccaacacaga aaaacctaaa 1620
ttcaaagatt tggcaaaaaa aagccctgtt ttgacaataa accctttaat tagttgaaac 1680
aattatagaa ataaaaggaa tatttggcca caaaataaat aaatagtttt agcaaaggtg 1740
tgttttcccc tgtgcattac tcaatcataa attttacaaa aatttatagt caatgagatc 1800
cataagaata cagagaaatt ttgaattggt tagaaatatg tttgtttttt tcgataactt 1860
aactaacaaa atcgaaagtc ttatgaattc aaagatatta tttagtttcc aataattatt 1920
ttacttttga tttttttttt gttgaattaa tcattaatga caaatggacc tacgaagaat 1980
gccatcaagg gtccttatta acaagtacca acaaatcaac aataggctcg tactcctttc 2040
taatttttcg gcactttttt gtcaacaata tttcagcatt tatatacatg gttttataat 2100
taaaagacaa attattattt gtaacatatg tctcctacta caccgaaaat taccggaaaa 2160
aaaaacataa tgtacatatc tcaaaagaca aaaaattaaa taaaaacttc aatttcaaag 2220
tagtaacttt cattttaaca aaaaaaaaaa gtagtaactt tcattcaagc atataatttt 2280
tctgtaagat gcttcataac taacttttaa tcattttcat taccattaag caaaagctat 2340
aaaatcccat aattcatata cataacaaaa taacaatctt ccatcaaata acttttatct 2400
atgatattaa aaaaaaagag aggccagatt tgggggtggt ctattcatac taaatgcaaa 2460
ccatctatcg actaccagac tgcattaaat gtaacaagta acaacaacca aaaaaaaaga 2520
tgaaaattca ccacatcaac agtattggac cccactttct tcttaatttt tcattcaatt 2580
cttctcccaa ccatctcttt gctgtcttca taactatctc atttcttgta cgaatcaaga 2640
attttttttc atttttgaaa ccaaataaat aaataaaaat ctcattatct tgttagagat 2700
aaaaagagaa acaaccaaaa agaaagaaga agaagtgttt gttctgtttc agtcattcaa 2760
gttcatgaga atagaaacga gtccaagttt gttgttcatg caaaattctc atgactctgt 2820
tacactcctg aatttcttct acttatattc cattcaataa cttttcttct tctatcagtt 2880
caacgttcac acactttttc catttcctcg acaaactcct ttttttctct cacttttctc 2940
gaattctttt tttttttttt tctctcaacg aaacataaat cctctgtttt ggtttaagag 3000
<210> 70
<211> 3000
<212> DNA
<213> 拟南芥()
<220>
<221> 启动子
<222> (1)..(.3000)
<223> 极长链脂肪酸缩合酶 (KCS1),蜡/角质 启动子, pAtKCS1, At1g01120
<400> 70
tatgcttaaa agttaaatct cttttgcagt tctacaaaaa catctggatt ggaggatgta 60
gtctggaata ctgatgcagc cgttgaagaa caaaagcaaa aaaaaaaaaa aatagcaagt 120
tgttcaattc aaagttttta actaaagttg aatgtataat gaatagcaaa aaatgctgga 180
gttggcaaaa tagaactatt ctttacaaaa agccaaacct agaagttgct acagatttag 240
atccatatgt tagccaatag ccatatgcga tcatcctcaa aactcgggtt ggcttacttt 300
gagttagtca gacctatgag tcaaagttca aacaattcaa ttagtcaaaa atactcggga 360
agttcgatat ttgagtatag ttgaatacaa agttgtcact ttatggctac ggttggcaaa 420
gcgttacagt gacacaatat cgacaacaat ttggaaacac acaatgatag aataatagag 480
agaagcaaaa aagttatgtt agctataaat ctacgaacca agtatattct gagtataaag 540
ttttgttttg ttataaatgg gattggttaa taatttgcca atcaagtagt gattttacta 600
caaaatacat aatgctggat atatagccaa gaatatggat atagatggtc catgtctacg 660
acaaaactag caatgaaatt gtggcggata aaaagacttg atcatttgtc gctattatgt 720
tgctatttgt taatccatag atttattcaa aacaaatgac tacgatttgg caacagaaac 780
aattgacaga atttatttat ctggtgatat gatgatattg acattttcaa tatcgaattc 840
gtagttggat tcggattaat cgtgaccaaa tatttataat tccatatatt agcattaatc 900
tttcttattc gagattaaga aaaaattcga gtaattaaaa aaaaaaattg tttgaaaaac 960
cattaaaatc aataatattg tattatatac agttatatac tgaaattcaa atacaagacc 1020
acgacaaaat gtttactcta atgtttcaat ttaatgaatc attcggtatt ttgaagcatg 1080
ttagagcctg gtagtactgt gcatgttata cagccaatat caaccttttt cgctagtttt 1140
atgtaaggct atatcgttta tttatgtagc aaccatttaa attgttagtc ctttttaaaa 1200
ttattgcaag atgtgagtgt gtacattcat acattgatga cggactggtt tctagttagt 1260
tggtattaag ccgaccaaca ttaattgttg gtttatgtct acacatgggt actagtctac 1320
catttattat catcattatt tcatttatct tgcaaattaa tactttgatc tgtttattga 1380
ttaattctct ctattgttca ttatgctgta cataaaccta aatatatgaa ttattagcat 1440
ctgatgaact ggatctttcc aatattgaag tttgagagtt ggtatgcgta ctgttgaaac 1500
atgtgtttca tgcagtaatc acgatattat ggaaacgacg tcgctgcctt ttgtctttgt 1560
tttctttttc tctattattt cccagtaatc acgatattag aaatgattta actattaaaa 1620
gcattacatt ttaccaacaa ctttatccca atgaaaacaa aacagggaca ataagtccac 1680
tttgggacct caaatccgaa cattttattt acctacaaag ctacaagcta caactacccg 1740
tccgaggtct attttggaca ccttggcaca atcaatatga aataactaga aaaatactcg 1800
aaaactaact agatatgcaa ctccaaaact aattacttct ttatcagatt tataatatga 1860
aataaattca ttatgtaaag ggtcccttat ataacaaacg tcttaacgaa gacagcaaat 1920
ttataaatga caatgactac aatactggaa cagctaatag ggatggtata taatggagaa 1980
acaaatcgaa atgtgctgct attaagaagt catagtaatt cattatcatc aatttaccat 2040
ggcccatatt aaggagtttg tatgcatgtg ggatacacat ttatacaaag aaataacaaa 2100
tttgaatatt tgggacacgc tttcgtctac ccttgttctt gcaatttgga cagtgaacgc 2160
ttctcatgaa tgagaggtac gtttctcgaa cacgttctcc ctttaaattt actatttaca 2220
acatattata tcaacttacc cgtcgctgac ccagtgcatt tgcactacgt gcagccaacc 2280
tgcttataat attcaattga tatataattt tataacttgc cctcactcaa ttttggataa 2340
cctacataca cttgctaata aaaacatcat ggatgtctaa aacgtttaag tttaattata 2400
aggaaatata taacaaacat gtcatatttt gttgtgttgt agtggtataa ttaacaagtt 2460
atgttgttta tcaaaaactt caaaaaaaaa aaaaattagg ttacaccaaa atagaatgtt 2520
atgatgccca aatatatgtg tgaaaaaaaa gataaaccaa atttatgatc taccatatga 2580
cttatgagat tcagctaggt tttgttagac ttttgtaact tgcatttcgt gtgaacaaaa 2640
catgaaaaat aatctgacta cttaatttaa tatcaaggtc ccatataagt agttgagcta 2700
atagtattat ctcggaacca cccatcatta aattcgaata ttattccatc tttttttttt 2760
ttttgttaag tcattactat tattaatctt taaaatattt ttatctttct tgcactctat 2820
atatctctcc atccctactt gccctcattt caccaaaatc ctcctaattc accaatttta 2880
gaattcccat tctcattttc tctctctttc tatttctcca aatttgccta aagattttac 2940
attttatctt caatccttcc ttgatttacg tttgaaatat ttcgacccaa aactatactg 3000
<210> 71
<211> 3000
<212> DNA
<213> 拟南芥()
<220>
<221> 启动子
<222> (1)..(.3000)
<223> 极长链脂肪酸缩合酶 (KCS2, DAISY), 蜡/角质启动子, pAtKCS2, At1g04220
<400> 71
cgttgcgttt cataaaaatt gtaaacagct ttccaacaga cttttttttc ttaataggct 60
ttaaagccca acgtcataac tgtctttatt aaacatgata aacggtagca taaaatctag 120
acgaaaaatc aacattgtta gacatgtaaa tcatatgtta atagattttt gattattggg 180
tacagaattt gtggttgtta tagctacatg catttatata tgtagtacta ttcaactttt 240
cttgttgtta catgatttct atacgcttga ttcgcattaa tcacacttgt tctattcatt 300
tgttccttgt ttagctatca ccattcgatc tcgtatatgt acccaaccca agtactgagt 360
aactattttt ataaatttat ccaagtacga gtgaatcaag tatttatttt tttagaccaa 420
cttttattct ctacatatac ttgaatcttg ggatacgtat aatataaacc aaccctcttt 480
agattttagg attgtatttt tgtgcataca cagaaatctg agcatagaca aatggataga 540
aaccacattt gctttatttc tatagagttt ggacattttt tggctatgat tttgtaaaat 600
tgttgtgtgc tccgtgttag cgtttgccga atgtgacact aattttgatc gaacaaacaa 660
aaaactgaac ataaattaat taatttgcac acatgtttaa catctataaa aaaattaaaa 720
aaaattgcac acataaatat agacaaatgt ttggaataag taaaacatga atccaagtgt 780
tattaaaatc agtattttgt tactacaatt gcaccaacaa aattgtatgt tcaaaaattt 840
agcgcagtac gaatcattaa taacttgtcc ctaactacta actatattca gatgtatgcc 900
taacttgtaa atgttactca tggttgagta gttgaatgtt gaagagctta cgttataatt 960
aaatgctcgc tttgattgat gcaaacaatt tcttcactat aataagcgaa aataatagaa 1020
ataataaaga tggaaaacta acaattaata gtagaactat atatatatat atccacatat 1080
aaattctttt tttttttgag gggtggttta aaactagcta tttagctgga tgctcggcta 1140
ttcggatttg tgtaaaatac ttctaaatat tgagcaatag gtaaatatag tgaatattta 1200
accatgacaa acaataaaac acacacacat cagaggaaac ttgggaatga acgtgaacaa 1260
taaaaaaaaa attaataatg ataataatag tgggcataga gatggttctt caacgaatta 1320
tttcttgccc aagtagcacc gtttggttag aaattcttcg acatttatag ttcaattaaa 1380
aattactact ccataatttt gggtgactta ccacacaatc tttccacaaa tcttcgacac 1440
gcaattcaca acagtatcat agtttattat cacactttag taatttatgc caaacctcac 1500
tacgcaagca aactatgatt cattagatgt gtggacaatt acgacgacag caaaaaaata 1560
atatgtgtgg acacacaatg acaaactcac attgaactat cttgaaataa atttatacta 1620
aatcgtagcg aatatataat cagtaaatag aagttaaact gaagatcata atcaactaaa 1680
atgaatatcg taatcaaatt gatgataata atgatttcag acacaataat ggtgacggtg 1740
ttgatgatga aaaggatctg tggcataaca actgattgaa ttcaatgcat cttcatgggt 1800
tttattccgt tagctttatc tttcacgtct ttatattctt taaactttgt tatgttattg 1860
acatttaaat tcatttgtat gcatgttatg aagagatcgt ataactacaa aaatataaag 1920
gtaaatcttt cattttaaaa agaaaatgtt tctatacgtg taaataatta gtaaataaca 1980
catatttcct cctgcgcgta gcagttcaac aaactctctt cttcgcgtag ctagaagctg 2040
caataacata aagaaatgat atatttataa attatgtgaa aatgaaaaaa aatattagtc 2100
cgcaatatcc acgtatcatt atgcgccacg taaacaaagt ggacccagaa taacccttgt 2160
ttccagtaga aggaatcaat cagtggcaag aaagaaaata atgaatttca ttaaatcgcc 2220
actctcgagc catcaaagta tcaaacaatt gattggagtt agttgtatcc ttcgcctaca 2280
aatttttcct tactctgttt tataagtata taatagttcc actaaaatca ttttggatac 2340
aacctcaata gatctcagcg tattgtttcc tgtatgttca atgaatccat cctccatgca 2400
atctggagac tggagtataa aataacacgt atggtatggt atttatttct catagctagt 2460
tccttaacag ttggaagaat ttatacatga cccggccctt taaaacctat ccctactaca 2520
agacatttgg gttactttcc atcgactttt gaatgtgcat caggcatgaa aatcctttaa 2580
ttatgcatat tttataatca atgcttaaat tactataaac acaaaaattg tagtgatata 2640
ttagaaagat aatttaaatt gttacattga aaagataata aaaaattata aatagacatc 2700
tgatataaaa atggatgaag tatagcatat taaaaaaaca tatgtttttg gtcaaaacag 2760
aatcaatgca tagttagctc accgctacaa caataaccat agggactacg taccatccat 2820
aactacattt tcttaaattg catcctcttt ctaaaatttt gcctataaat tcacaataac 2880
acttcaactt tttaggccat aagttatctc tttctctaca ataagcaata aatctcacct 2940
cccttttttt tttttttgtc tcgctacttt tgattatcat ttaaaaccaa aaaacctacc 3000
<210> 72
<211> 3000
<212> DNA
<213> 拟南芥()
<220>
<221> 启动子
<222> (1)..(.3000)
<223> 酰基载体(FATB),蜡/角质启动子, pAtFATB, At1g08510
<400> 72
cactatccac attaagattt caataaaatg tctcaaaatt tattagtatg aatttctaat 60
atgatattta ttgttttata aatttattaa ttaacctaga aaaataatta aagaagtttt 120
tcttattaat tggagagtta cgtttttatt caatttatat cttttaggaa caaatagata 180
gcgaaggatt aaattttgca taataaatca tgaaatctca aggtttaatt aagtcaagag 240
tatgggaaat gcgaattcaa tcgcttggat gtagtaaaca agtggtaact aatatagacc 300
acaaacatta ttagctttga ctataaaatt gatcctcaag gtgaaaagta ggctaactcc 360
tataaatctc caaggcaaca tccttaattt tgagagtcat tcataagaag aattgtgttc 420
ataacttttt ttacttgatc agaaaataaa ctatgcaata agtaataact aggttttcaa 480
aaggcataat catctgtact tctttgtata tgaaaaataa atatgcaaat gcaactaaag 540
gttttgaatc atcttttttg tttaattaag actttttaat gtaaaccaaa ttaaacacga 600
tatatattca aaatgaaaac agaattatca acgctgctgt ttctcctgac ttaattcttg 660
actcgtctcc aagcaaatta aagcgtccaa atgagagatt taataataat caaagataag 720
taatatcatc aatgacgaaa tagtcaacat aaaataaagt ttattgtgat ctagtcgatg 780
tctactgtac tttctcaatg gttatactat tattggtccc aggctcctct tgtagcgtat 840
gcgaggacta ataggctgct ctcatggctc attttagaat tgtaagtacg tttcgcttaa 900
gtctccttct ccatatggct cttcggaagt actagtagat cacatgctct acaaagtttt 960
ataccgtctc cttcaagacg tatgctaagc atgcttgtgc gagtgtgcat ctcccacacg 1020
tctctcgatc gtggtagtta tctatgtccc tagtatatgt gttgtcaaac ttttattgat 1080
ctacgcttgc catcatctca tgcatgcatg tgtctcacat ttattatgat cgtcaaattt 1140
aaccacgaat atggagcaaa caagagacgg atatggtcct tgtaaagatt ggcaatcata 1200
gtcaattcca tgaacctggt cctcaatagc ttatcatatc ttcttgttag cctcttatac 1260
gctaccaaaa tgatagctga aggcaaaatt gatctctcca aactggtaat tatatattgt 1320
gatgttcttg ttgggaagtg tatctaccta ctctcctaaa catttggatt ggctatattg 1380
tttcgatttg aaaaaaataa cacgttgctt ttgtataata acattatttg tatagattac 1440
ttggtacgta aaattttgaa gaagttaatt taaattatgt tttcggtgga ataaatatat 1500
aaatccgaag gctgacttta cacgttcgac aaagttttat ggttaaacta aaaaaatgag 1560
aaaaagctag agttaggttt agtttaaatg tagttttcgt tagtcttaat aaatacaagt 1620
aagatagaga ttgatgcaca tgtaccttgt tgtctctgcc cacttattat tacacagcgt 1680
cggtgccagt attgacaaca agcttgctca cacattcgga tatgtgtctt tttctctcca 1740
attacaaatt tgattggttc gatttttcaa aaaaaaattt ccaagtccaa aggaaatttc 1800
cacggaagcg caaatcaagt tttgcgtgct aaatgaaatt caaagaaaat ttgtagtcca 1860
tatgaataaa aattgaataa atttgcctaa ttaggttaac caatcttgta acaagaaaag 1920
ggcaaaatgg gaaatataga aaatgaaata aaaatgtgtc gttgttgttt tgaaactatg 1980
gagaagaaga ggagaggcaa ttatataaaa accgactctc atatccacat atatctctct 2040
ctcacccacc ttatagaatt ttctcgccct atttttctct caacaccaaa aatttgtatt 2100
ttttttttac tattgtttac cccaaaaagt attgagaatc gcaaaaaaac tgaattgtaa 2160
aaaatatttg agaaagagaa atacaaaaaa aaacaaaaaa aaagttttaa agaaaaggcg 2220
aaggagagat cgtgagagac agagaggctg ccgaagaaag gagaatttag ggtttggaga 2280
cttttgttga aatttggcgt tacgtttttt ttgtatttcc tcaacaaatt tctcatctgc 2340
tctcttcata ttggtaagat ctctctctct ttcatttttt tgtttttttt gggttcttct 2400
tctctttgtg taatttctga atctcttcgt cgctggttcg gattcttcat ctgattgatg 2460
tttgattgaa gaatgatgtt tgaattacat tttttctaat acaagttttt gtttttcttt 2520
tgcgtttcta cgatctggtt ttaccggctt tagctttttc tcgcttctgt tctttgttgt 2580
tttgtatttc agatctggtg ttttttttct tacctgcatc aaattggttt ctaccaaaac 2640
ttcggaaacc tcttttggca aatgttgaat ctttgaatac aatgacgatt tacataatag 2700
tctcagtggc cgaactggat tatcttacaa tttacgcaat aacaaaaagt tttttttttt 2760
tttttttttt tgtgtgtgtg tgtggtgtgt tgaagatttt tagtgtttgt ttacttcgtt 2820
tatggaagtc cttttcctct tctgccattt ttgtagttaa ctacaaatta tacctacttt 2880
aggaagatcc tcctgctagt agctaaaaga tgtagcattt attttattat cactcacttg 2940
agctaacttt tttcgatctt tatttggtgg cagtgtcttt gaacgcttca tctcctcgtc 3000
<210> 73
<211> 3000
<212> DNA
<213> 拟南芥()
<220>
<221> 启动子
<222> (1)..(.3000)
<223> 长链酰基辅酶A合酶(LACS1),蜡/角质启动子, pAtLACS1, At2g47240
<400> 73
aggttggaaa aatttaatga tacattgtac gttttctagg ttttgttcta aatgctcata 60
aacctacttg gatttggaaa taaatgagag cttacatata tagaacaata ctctatttcc 120
acaaaaatta tttgctactc atatacaaat gtaggtttag aaaaaatcca ctttagcaaa 180
aagagatggg aaattaatga aaattcactt gactttcttt gttttgtgag caattttcga 240
tttcaaagta atggtcggac ttagactttt tcctgttctt ttttgctgac ttaataataa 300
gctgaaactt aacagttaga agttggaaca agatataaat aaaaaagcta agttgtcaaa 360
cataaagttg aaaatgattt ggtgatgtca attaaataaa aagttgaaaa ctaatcattt 420
gtatttgacc ggcggttact tggaataaga ctaagaatta ttgtactttc tatttaatgg 480
gtttgctaag ttttttatca gatcatttta agcaaaatat cgataacttt tcgaagagat 540
tagttgaaaa tactaagcat tatatatatt taaatatgat ggatcagtat atatagtttt 600
gaaaatcaca ctgcaggatc cgttggaatt ggtggctgag aacttttatg atcttcttaa 660
aattttaatc cgtaaataaa ttatacgagt tggattggtt cgcgtcttct aatgcgtatt 720
gacatctata ataaaagcta tcagaaaagt tgctgatctt tttctgtatc ttattaaata 780
tgtagacccg agcagagaaa tgtggatcaa ttaataaata tcatgtacgg atgaacaaga 840
gagaagaaaa aagtataata atactataga gcgaaaagta ttgcatgatg gagaaactct 900
caacaatttc aaacatgaac gacttcttac aaaacaatgt atctttagtt gtcacaatct 960
ttgaaattct acaaatagag aagacggaaa aagatgaaaa tgaaattagt gtttttcttt 1020
ggcaaggtcc ttggatctct taaaagtgat tagatcaacg gtaatgtttt agatatccta 1080
catgtgcatt gattcgataa aagcatttaa atagtgaata ttacaaacaa aaatagatac 1140
ttacatcatg aaatagaaac aaaatcaaac tgggttgcta cttaccagta atcacgtgac 1200
ggtaactggc taactgcaat agtatatcca aaacaacttt aagaaatgga tacgaaatgt 1260
tttagttttg ggaaaatgtc aaataaagat ttcgtcacaa atccatcaat ccagtataga 1320
acacttttat caaaatgatt ttgcaaattg attcttagag tacgatacaa gttgcaaact 1380
ggaaaaataa ttttgcagtt gcaacaaata ataataaatg attgtgcaaa ttgattctca 1440
aattaagtag gaaacaagtt gtagtagata tagactgttt ttacatgatg ttaattccaa 1500
aaggattcat tgccatacaa atggttttct catagaggac gtaatatcaa ttaatggaaa 1560
atagtagttg aagatttata gaagaaaaaa aaaactttcc gcaaacaaca ctatatatat 1620
atatattgga tcaaaaataa aaagagttaa atgaaagaaa tacacagacc tggcctgtat 1680
caaccaactc atatattatt atataaacca acatccattt ctcagctcct catcaaatta 1740
tcttctttct ctttctctct gatcatttcc aaattatttc taagctaaga aaatctttaa 1800
aaataacaaa gcttctaaaa taataatccg acctaccgga ttcaaaccat tgatctgaag 1860
atcatctata aaccagtcct tgatctataa actaaaataa ggtcggttgc ttaatctttg 1920
ttaccctttt atttttgtgg ttataattat attatcttca ttttgtttcc ttttatgatt 1980
aatcgatcat atcaggatag atatttcgtt aagtttatag tttaaatcca gaattttatg 2040
tttagaaaag agaatgggaa gagttgaagt gtttaacgtc tatgaatatt cctcacgtca 2100
agaaaaaaga aaaaaaacta tgaatggtcc tctctcgatg tccttacatt ggttagagag 2160
cgttgcaact acaatacata catcactttc atcatattta ttgtcttttt cagaggtttt 2220
catatttcga ctgaattctt caatttgcaa tattatgaat aatagagaag atgatgaaaa 2280
aaggataaag agagtgaagc cagggttatg catgctctca cgtttagggc gtgactctaa 2340
actagcgatt tagtttacgg tattattaaa aataatatta gtcacataat atttgtaatt 2400
ttagtcgaaa ttaatgacag aaaaatacta gacgctagga tttgtacttc cttgaatcct 2460
tggaaaatat acgtatgtaa ttaatattat tcattatcaa accacaaata cggaaaccaa 2520
gtttgtgtca aatccaaatg gagtagtttg gtatcttagg ttgttgttta tcataaatga 2580
gaaactttgg ggttgcactc gatcggtatg gttaggtgga ccggactggt gacaattaat 2640
gccattgatc tataactgtt agattataat ctaaaggttc cattgcagaa caattcatca 2700
aactttaata tctatgtatt tatgtatgtt cccaactcac agaatttcac ggaattgcaa 2760
aactatgata aattctgcta ccttaatgaa tacactatga acttgactaa aaaaacaaga 2820
ataaagtatg ataaattccc tcattttata tttctcttat ctatttcaca tttttgcttg 2880
aaaatttgta ccattaatct ctctattcgg cagtgttaaa ttttattatt taactccgtc 2940
ggttggattc attttaaatt tctcttcagg gaagaaaaaa acaagagaga gaaatagaga 3000
<210> 74
<211> 3000
<212> DNA
<213> 拟南芥()
<220>
<221> 启动子
<222> (1)..(.3000)
<223> 长链酰基辅酶A合酶(LACS2),蜡/角质启动子, pAtLACS2, At1g49430
<400> 74
cgatggtttc gatgagctta atagctccac gtcctttgga acagagtttg tccatcactt 60
tctcaggact gcagcaaacc actttgattg tgaccgtgtt ggtttcttct tcgaactttt 120
ggtctcttac ttcttttgtt ttcccaaaaa aatcacataa aacaaattat tattatgagt 180
ttttaatttc aaaccaaaag agacgtaaaa ggagagaaag atataacaat aacatacgag 240
gtatgctcga aagagccttc ttgactttcg agaaattctt ttcgttacta agaggctccg 300
tcttcaattt catccatgtt acctagttta atttcatcat ataatgttct catagctttt 360
aatacaactt ttgtaacaat tagtagtaac ggtttcaaag agaagcacaa aaaagaaaaa 420
gaagagagcg tttatttacc ttctccttac ccttctcggc catggctttg aaaagaaaaa 480
tattttcagt gtaattgctt gtatatacta acactactat gcagctatgc tatatatttg 540
gagtttcacg agagggtttc caaggaaaaa gaagttgaat attgtacaat ttacttttct 600
cttagaatgt ataatttcca tagctattat tccatattac attgtcctaa aacaatgtaa 660
attttctttt ctttttttgt ttaaagtttt tcctatcttg tttttttagt tgaaatttca 720
aatttccttt tgttaacaaa aagaaaatat taccaaaaaa caaatgatgg ggcttagtgg 780
gggttctaaa gagagaaaca aagcatagtt ctattctttt ggaagaggac taaaccctgc 840
tgtttttttc ggagcgagga tttacgagtt ttggccaaga actttcggtt tttttttttt 900
ttttgaatag tcctagaact ttcggttttt taaaaaataa tactaatgta atagaaaaaa 960
aatggtgttt cttcggagaa aaatgacaac gggtggcaaa ccaccttcga agctatccct 1020
tgagcatgtg cacgccagtg tacttaagtg agacattaag ttccaaatca atgcgaacga 1080
gctgagagga ggagatccca ttacgactgt gtcgactcaa atttctcttc ttccataagt 1140
aatagatcac tgattggaac caccttgtga tacaaaacat tataatccca tttgatgtat 1200
tttacagaga ttagatgaga aaccgtgtct gaccaatgat agtcggctag aatgtgaatg 1260
tgtaagaaca cacaaaacaa aataaacctt ggtggaaaaa ttattattca agcgttaatc 1320
gtgagaagaa aataatattc tttcttaaag tattgtgaaa acataaactt ttcaaaatct 1380
aaaatatgtt aaatatttat ttgaatttac tttgtagttt aaccgtgtaa aaacttttta 1440
cctataaaaa agaaaaaaaa ataataggga taagtttttt ttatatatac ccataataat 1500
taaaacatcg ttcaaatttg gatattttcc ttataaaaag ttaaagaaaa agcatgtttt 1560
tgtaaattta attcatagaa aatgtctgaa attttccatt atttataaag attatagatt 1620
cgccgaaaat taaattgtta aggctgggga agaattattg ttcacgccaa caatcacgtt 1680
atgacataag attcgaaagt tagcgttaaa tttgatgttt ccactgttac caagcaaaaa 1740
aataaacatg tcaaacaaaa gaaaatggac tacaaatttg atagaaaaaa atgtgaataa 1800
aataactgta gtcgtttatt tacttagagt tgtatacata ttgactaaac acaataagaa 1860
aacaatttta tagaatacta taggtcttaa aagatagtat attttatttt catttgatga 1920
aattacatat ttgactatgt ttctaattga aaaaaaaaaa atttattaaa atatccataa 1980
aaatgaaatg ataaaattta ttaaaattaa atgggctgac cttgtaataa tatagggagc 2040
aggtcggaga ctcagagagt gagcttttct tgctcgtcca tttattaata atttcattaa 2100
agagaaaatc tgtagagttg acacttttta aagtctaatt attagggata tatacgtaat 2160
ttggtttgaa aataaatatc aaaatctagc taacttgata tcagtatggg taaaagatag 2220
gagtaccttt tgttgttgat ttaattgacg atctagtgtt aacccagaga attctaatta 2280
gattagcctc tttctctcat ccgaaagttg aagtttaata agttaatctg atatttgtaa 2340
ataacttgaa cagtaaatca taaacaaaga aaaactgatt tggttgcaca atttaattag 2400
tgaggtgagg tctatcacaa tcactggtta ctatagtgat aaagatcctg attatgacag 2460
tgactgtgag ctggtgaggt ctaagagtac aaaaacaaat gatctaagtt atcaaactat 2520
aaactatata attcgaaata tattaatttt gttaaatcca aaaccaaatt gcatgcaaat 2580
ggtcagtttg taaatagtct acgtttggtc caattactct ttactcttaa ggttctttac 2640
tatattcaaa cccactaaat ctacttgcat ataacacata gagacacaaa aattagtatt 2700
taaaacattt agataacaaa caaaagaaag agaggctcct aataaaggcg caattaaaaa 2760
ccggtcagaa atacaccaat tgtgagactc aagaagttat tgctcaacta cttcctctaa 2820
ctcataacca caatctccaa acttcaccac cgattcacag agacttttat taaccttcaa 2880
aacattattt attattccaa tcttcaaaga gagacactca cactccttat cttcttcatt 2940
cctccatcct atttttcttc ttcgccaaag tgttattcct caactttata tattacacaa 3000
<210> 75
<211> 3000
<212> DNA
<213> 拟南芥()
<220>
<221> 启动子
<222> (1)..(.3000)
<223> 细胞色素P450依赖性的脂肪酸羟化酶(CYP86A4), 蜡/角质启动子,pAtCYP86A4, At1g01600
<400> 75
acgatatgct ttcttcatct tcttctgatc aaatcaatcg cctcgctgtt tccgtcgagg 60
gaccctacgg ccctagttcc actgatttcc tacggtaatt cacttaaatc acaaacagag 120
aagttcaaca ttctgtttct tccgacacaa gtaacattga tgctctgttt ttcttccaac 180
acaagtaaaa tgctctgttt ttctttaagt gcagacatga atctctggtg atggtgagtg 240
gaggcagtgg aattacaccg tttatctcca tagtccgcga cctattctac atgagttcaa 300
cgcacaaatg taaaatcccc aagatgaccc tgatctgcgc gttcaaaaac tcttccgacc 360
tatccatgct cgacctaatt ctacccacct ctggtctcac caccgacatg gcctcgtttg 420
tagatatcca gatcaaagcc ttcgtcaccc gagaagagaa aacatcagta aaagaatcaa 480
ctcacaatag gaatatcatc aaaacccgac atttcaaacc aaacgtctca gaccagccca 540
tctcaccaat cctcgggccc aactcttggc tctgtcttgc agccatcctc tcttcttcct 600
tcatgatctt tattgtcatc atcgcaatca tcacgagata tcacattcac ccaatcgatc 660
aaaactcgga aaagtacact tgggcttata aatcgctcat ttaccttgta tcaatctcca 720
ttaccgtggt gactacttct acggctgcta tgttgtggaa caaaaaaaag tactatgcga 780
aaaatgacca atatgtcgac aacttatctc cggtgattat cgagagctcg ccgcagcaat 840
tgatatcgca atctaccgac attcattacg gagagaggcc taaccttaac agtaagtcta 900
tttataacat tttcatagag taaaatgctg agttctaact ctgtttattc tattgtgtga 960
aacagagctt ctagttggtt taaagggttc gagcgtggga attcttgtgt gtggtccgaa 1020
gaagatgaga caaaaggtag cgaagatctg ttcttttggt tctgctgaga atcttcactt 1080
tgaatctatt agttttagct ggtgaatttt aaattcagac tgatgtttta tcatagatgt 1140
ttgggttaca aaagaactag gtggcttcga gagttcataa ctctaaacat gtcctagtac 1200
taatttatcc gtctccggtc tcctagtaga ctagtactaa tattaaaatg tgaagcgata 1260
tatgagtcac atgttggcag gatttaaagt ttgacagatc tatatgcggt ggatacgagt 1320
tcggttagct gaaatttgta gaacaagcat ttatgtcatg aacgtttctg aatggtttta 1380
taatggtaat tagtgaatgg tttaagacat tctttaggca gtcatgtagt tttgtaatat 1440
caaggaaaat tggcttggct ctgaatatga tttttttttc tgaattggct tgttcgaatc 1500
ctcaagatga ttttttgaaa tgtaaagttg cattttctga tataattttt ttttgttgcc 1560
ttttataaat aatcatcttt tctttaacat ttggttgcct tttattaaaa atcatctttt 1620
ctgatttttt ttggtcaact aatgtaacac cttttcttta acattttttt taaaaaaata 1680
aaacgcaaat gaagtcatgg taaccctttt gactttcaca ataccattca gtgacatctt 1740
tcttacttaa tattccgctg tcatgtccca ctttggttaa catttgagga atttcatcag 1800
taaaaatcga aaagatataa aggcatcaaa ggagaaacct taaaaagtca aaatagagac 1860
acaagaagtg tttctttgta ggcttagatg agaagaagaa actaaggaaa ctgtattcct 1920
aaagtaaata ctacttcctt cattcctatg ataatcaatt attaaaagat atggagtcgc 1980
ttaccaaatc ataaaaccgg taacccaaat ctaggaaatt ttattgtgga ataaataaca 2040
aagcctttat tttatttaaa tagaacaaga taaaacacca aaaataatag ctatgttctg 2100
aagtcttgac caaactactg atacgggatc tcacctagat tacttacaat gtaggtgtaa 2160
accgagtttt taaccaaact aaaacgaatt aagcgacaat tagttacgga agcttaaact 2220
ctacatagat tgatagatta aaaaaccctt gaacaaaaaa agaatccaaa ttcagcttga 2280
agtagattca accaaaccga aataattttt taaaaatatg aattttgttt cagtttgaat 2340
ttcatttgat tttccaaaaa ttcacaaacc aaaccaaaaa ctgaatatca cttgtagctg 2400
tacctataaa ttattagagc attaaatcta tctcaccacc gccaaactca acgtgtgtta 2460
atcttttaac cctaaacttc taaattaaaa aatagacgac ttttttactt atttcttctt 2520
cttccccacc atcttttttt gtgctctatg ttctcaacta atctcattta attcaccaca 2580
tacagctgca tcaaacatat atatatatgc atgtatttat gtacttatac acatacatac 2640
atatgatcat ttaatgaaat tcattaaatg aaactattcc tttcgctgca aaattaggta 2700
ccagtaaagc ataaacacca ctttaccact tcttgaattc caaatctgaa agctctgtca 2760
ttatatatgt ataaaccaat ttaaacatat acatgtctca ccgataacaa cggaggttca 2820
ttaatgaggg tttgattatg atacttacga gtgacagatt gtgaatatca aagagaagct 2880
tcttcctccc ttttttctcc ctataaattt ccgtagaaaa atcattcatt cgcatctctt 2940
ctcttctcaa aaatcaccac atttctttac ttctctggga aacaataata aaagagctac 3000
<210> 76
<211> 3000
<212> DNA
<213> 拟南芥()
<220>
<221> 启动子
<222> (1)..(.3000)
<223> 细胞色素P450依赖性的脂肪酸羟化酶(CYP86A7), 蜡/角质启动子,pAtCYP86A7, At1g63710
<400> 76
cttcctttta tagctccacc ttcatctcca gcttcatttt tccaatcaga gccaccttct 60
gctacacaat cacctgttgg aatcctctct tttagtcctt tgccttgtaa caaccgtcct 120
tccatcttcg ccattggacc ttacgctcat gaaactcaat tggtatctcc tccggttttc 180
tcaacttaca ctactgaacc atcttcagct ccaatcacac ctcctcttga tgactcatct 240
atctacttaa ccaccacaac accttcttca cctgaagtgc cttttgctca gctctttaac 300
tcgaaccatc aaaccggtag ctatggttat aagttcccaa tgtcttctag ttatgagttt 360
cagttttacc aacttcctcc tggtagtcca cttggtcagc ttatttcccc gagccctggt 420
tctggtccaa cttctccttt tcccgatgga gaaacctcgc tgttccctca ctttcaagtc 480
tctgatcctc caaaactgtt gagtccaaag actgctggtg ttacaactcc ttgtaaagag 540
cagaagattg taagaccgca taaaccggtt tcattcgatc ttgatgctga tcatgtcatt 600
agatgcgtgg atcagaagct aagaacaacg ttccctgaag catcatcaga tcaagaatca 660
atgaatcatt cgtctctcgg gtccaataag gaattcaatt tcggcacgga tgagaaacat 720
ttgaccgttg atgaacatag atcagcttcg ccgaagaaca gcaatgattg gtcttttttc 780
cctgtgatgc agtcaggtac actaagctaa ccttcatcag aagaatagaa atctgaaatt 840
tagatatcga ttcggacaaa tatcttgttc aagattcaag aacaattata gaatttttag 900
atgattctgt tcaggatctt aaggatattt tcttgtctct ctttttggtt ttgtaataaa 960
tatttggcat cgttagttgt tgtatatggc tactctttat gtagtttttt gtttttgtga 1020
atacacattt gatcgcattt gtaaataaaa tttaatcagt ttcttcggag aaattccatt 1080
aaatttatag gtattgttta ctatttgatc ttctgttctg tgtattgttg tgactagagt 1140
tttgttattg ttagagccaa taaaaggtga gatctatagc ataaaggtag tgaatgtttc 1200
tccttatttg ttatcttaga ttcaaccatg atatgcaatg taaaggagaa aaagaaaaaa 1260
gagagtaaac atttgagtat acaattaaca agcaactcaa gactgttaac aagttttatt 1320
caccaaatcg atataccggt caggttttat aagaaacatc acatcacaac cactaagtcc 1380
actacaattt gaagctttga gccctgtaaa caaggattat acatgacaag ttagaatggg 1440
ccagcttaaa ttactaagtc cactctcagc attccgcaag cccacttgga atccagcttc 1500
aagttctcat aggcgctttt ggttcaatta atcccaccta aaaccagtat gcaattggag 1560
agttaataaa tcttgtaggt ttaactcttg ttaggaggat aaattagatg atttaaattt 1620
ccttatgtct ttttaaaaaa aaaaaataca attgagtctt gtcaatatgt atctcttttt 1680
tttttttttt tttttttaca gcaacgttaa ctactagtca gcaaaaaaca cataggttaa 1740
aatatgtcga gtctcataat gtgaaaacaa aagaaaatac aaagattgaa aaagaaacaa 1800
cgacccccac ttttaaaccc aattaatgcc gtattttaag ttgaagtgcc gctttcctca 1860
aatctcacta ttgtttctct tattctctat cattcatatt tcatagccaa caaagattat 1920
ggtttagagc atttactgat tttatgtcaa ttataaacat gtctttttta tcaaacgttg 1980
tgaataatcg attacttatt atatggtgct atttataaac ttactgcatt tctttttctt 2040
caaatattcc gttataaagg tgttgagaat tttgtttata aaattttaga attactgatt 2100
actgtacata catgtgtttg tggtattttg ttaattattg tcaattatca aaacacgaaa 2160
tgttgtctta ttaacccttt ttctgaatta ttgtgttctt gtggaggata tattatatag 2220
gtcgacataa tttattcgga tcacgcgtgg aaattatatt ttgccaaaac gaaagtagga 2280
attatatgat tcatgtatca tgttattgct atttttattt gatactgtat tttttataaa 2340
taaagttttt tggctcacca acttaagtgg cactaatggc attggcccct ttaagctgta 2400
agttaccatt aatttatgtt gccaaaatat atagctgtca ttatgttgcc ttagtatgaa 2460
tatatttttc tatcacacaa tatcaataga tatatggtta tgaccatatc aacttgtccc 2520
atcaacatct caatcaaaac atttgacatt atataattaa ccaattgatt ttaggatagg 2580
actctacaaa cttttattta aacaattttc gcctgaataa aatcatgtgt ttaattagga 2640
aggtgattat gcacaaactc ataagtgagg aaataaaagg ttgagatata gtaggtgaat 2700
taatatatac aatattaggt tattataaac acaatgacaa ccacaaaact ctacacaaac 2760
aaacacatga catgcaaatg caactatacg tccccaattt tttggttggg aagaataatc 2820
aacaacaaac ttagttgcct tattaattgc ttatactttt cttttctctt tttagatttc 2880
attttcacat tttttaatct ataaaacatt aatatgatta tcttccattt cacacacaca 2940
ctcacacttc aattctcttc ttatagattc tgctctcacc tccagactaa aatataaaaa 3000
<210> 77
<211> 3000
<212> DNA
<213> 拟南芥()
<220>
<221> 启动子
<222> (1)..(.3000)
<223> 细胞色素P450依赖性的脂肪酸羟化酶(LCR, CYP86A5), 蜡/角质启动子,pAtLCR, At2g45970
<400> 77
ccatttcaca aagatggtaa aaaccgtaaa atgctgatga aatcgcaaaa tcattttttt 60
tactaccaaa gagaaccatt gttacaccga tacaagcttt agctttagca tgtgtcgcag 120
tagtcgagac aaaatataaa tccgtcgttc tttagtttcg gtggtaaacg agagaataaa 180
tgactaatga tatagataat taagtaaaca tgtgattatg cgtattgaat tattagcctc 240
tagagtggtt gagtgttgtc tgaattacta ttaattacca tgtgaataag cgacgtggta 300
actaactatc ataatgattc attcattatt ttagctactt ttcaatatga ttttttttat 360
atctcctttt gattctatat atggtggtct taaatgccaa atgagacaag taacaagtaa 420
agtgaccaaa acaatttcac ttaaatgacc agttttacgt gggacaaccc acggaatcta 480
aagatttcta tgttgtttat tacttaacgt gataggttaa gtttgtttgt taagtcgtaa 540
gaatcgtaac catggactca ttaccattaa tgtgccaaat tccgaataga ttttgtagtc 600
atataaagtc attgcgttta ttcaataaac aagtcatagc tatcgagaat tccttcattt 660
tattttattt ttttggtaaa tagtgcatgg gatggatata cttgatgcac ctaatctaac 720
tattttattg tctattattt atgtaacttc ccgtacgtaa ctgtcaattt caagtataaa 780
tattaattaa tttgtaaaga tattattatt taaaatgatc tacaaagtgt attgtatatg 840
tttttatttt gccgttgatg ggaaggtaac ctaattagat aactttatat tctctatcga 900
tttgtgtcct ttgtcttaag ttttaaccta atattgcata tttaaccaca taaatacact 960
aacatgtgta aacattcaca ttctgcaagg atttttttgt ttttaaaaga acttgaaaca 1020
aaataaaaga aaaggatcaa ctgaaaacca actgtcaatc aagttcattt aaaactcacc 1080
ttgtttcata cagctgtaat gaaaaataat agtaatggat ttcgaagtat tttaaagttc 1140
caaccgggta ttctgcaatg cttggtttga aatttgaaaa agttgactat ggttggtcaa 1200
tgcattcatg ctttgatgac aaaaatcaaa attatatata taaaaaatag ttttcttcct 1260
tcacaattat ccactatagg gtttatatat actatatact ctttaattaa ttctccgata 1320
gttgttcctt ttgaaaaggg ttgaattttc tgatagtttc gttaacttaa tcgtttttct 1380
tctatgcatt aatttttcga aaattgaact ccaatgcagt acatgactac atcgtatttt 1440
aatattttcc cacagcttct aataatatta caacgggggg tttaaattca aaatttaaaa 1500
gattttaatc gtcgacatgt gaaaatgtag tattaggagt gtgtttatat atcccatgta 1560
cacatcacga gttctcgtat gagtatgtgt tggttcttac attaatcaat ttatcacgat 1620
cacgcattaa gaaggcgaca cattattata ttgtagacat tgaatacttg taacgtcctt 1680
tttaaaaata tattgacaaa tatgtcacca aacccaaaag caacctacat atgttgtgta 1740
gttgagcaaa actatcattg gtgagtttgt tcacctatac cctaacaatc tcaaatctaa 1800
aaccctaatt actcatccct atactctatt gtatcacaaa aataggattc tataatttta 1860
tacacgcaca tatactttgt caaagcgtat cataaaaaaa tgaaataaaa atcaacgaaa 1920
taacgtgata tggctagtaa tatagtacgt agtcttacga caatagttta acttatacgt 1980
gaaaaagtgg gataacatga gttttcatat ttttgtcatc aaatgatatg aaatagaata 2040
accgaacata gatcattttt tcactaattg agaagaagag aattagagac tatcgactcg 2100
tacgagtggt gaaacccctg tcattttgcc gttatacaat ctactgacca tgtccatgac 2160
taataataca tctatcaaca accatcatgt cccttgaacc gacaccgaat tacaaaaccc 2220
taacttaata tcttcaaaac atgcaattaa tagacacgag aataagataa aatgaggagg 2280
caacatccca tgcttacttt gtgacttctt ttacacgatt ttctaataca ttttaaaaat 2340
actacttgac atctctccat ttttgggggg ttttgaatta aattgtatat ataaatggtt 2400
catgcttgga cattgaaaac ctgtgggtca tttagtcata gatctctctc tatcttcctt 2460
cctcctcctc ttactaggct cctcaactaa acgtcttcaa tgctctctct ctctctctct 2520
ccattctttt acgacctcat agacttgaaa atttaccatt ctatttttca aaacctttat 2580
attgatgaga ttaatatata aagtctccaa ccacctagct accagcctag agcaggaaca 2640
ttaatggtgg tgcacaccat ctcatgcttc acatacccta aagttcttca ctccaagttt 2700
atgaggagat ctagagagat aatcatatag agaaagagag atctccagag aaaatccatt 2760
gaagggaaaa aaacagtctg tctttcataa ttgctctttc tataaatctt ctctcttgtg 2820
ttcttacctt tcttctaaaa ccattctctc tctctttaac acttcatcac tctttacgga 2880
aaatttctta ttttcaccac ctgagccatc tctattttcg tcaaaacttc tcaacttttg 2940
tgccaaaacg tcatttcttc tgagggaata aacacaaaag aacaattttc ccggaaaatc 3000
<210> 78
<211> 3000
<212> DNA
<213> 拟南芥()
<220>
<221> 启动子
<222> (1)..(.3000)
<223> 极长链脂肪酸缩合酶 (KCS10, FDH), 蜡/角质启动子, pAtKCS10, At2g26250
<400> 78
gtcgtgtttc gtttgtggat tttgttttct catgattttt aaaatcccat gtttacacga 60
tcataacgtt tcgtcaattt taaaacgata actctataag taatagcgtg taccttcatc 120
gtatttcttt cgacgtcgta attatgatca ccattcacca ttttccttct tttttctttt 180
tagggtatat aacgcaacta tataaagcac acgtacttaa agaaacccat ttgggcatgt 240
gttgtccttg ctgtggatca catgcataag tctgcttttt ctttttaata tgaattgttt 300
ttgacttcat atataggacc aattcaaaca tttatattta aagaatattt tactcagtat 360
tattactttc ttttgtaatg atttacaatt ttgtgccact cgtatgtata gcgttttaaa 420
gaggttactt accagtagtt tttgtccaaa ttttatatgt gaaataaccc ttctcggaag 480
gctaccacat ttccattaaa ttattggaaa acttttacaa aatgtgacaa ttcgatcgga 540
aggctactat ctcataggta ttgaggcagg atagtcacta agttaatgat tctgcatcgg 600
tcacaaacgt ggtgatcgac gtgaaaagat tgagccctcg caaaagtgag atggtgcttt 660
gtcaaacttt tgcgataagt gatcatgata attacaaatt aaacttcatt tttgccgttt 720
atgtgtcact taaaattatg ttttatttta gttttcttaa atgaaaaaag agagtttata 780
tatatatata tatatatatg aatcaaaatg ttagttagtg gatcttttga ttctgaaaaa 840
ttgttggata tttgtttcct cgcttaaatt ggtatttata gtagagtttg aatcgaggat 900
ttgaataggt tcaagaatta ttttaccctt tggaagcgtc atctcgcttt gagttctact 960
acgatgttat ggttagactc ccacggtggg agttcccatt tactcttaaa tgcaactgaa 1020
cgggctttac tatttgatgc aatgatacaa agaaccttca gatttaaaca atgagacgat 1080
cgcaacacaa gagaataatg ttactgatcc ttctacaaca aacactgcac caaggcctag 1140
tttgcttgat tgaatgaagt atacaaagca tagacactta tcacatttaa agtatttgta 1200
atatggcaat ttcgtatgtg ttaacacatt tctcaaattt taaaaatcgt ttatatggat 1260
cttataaaat atggtagtaa taatttaggt gggggattgg gtcattattt atttctcttc 1320
tattttgcta ctgcattaac taatattagg ctaaaagttc cttaaataat aaagcttttt 1380
tatgcgtaat gtagccgccc tcatttatat aggtctcacc accaatcgat taattgtaag 1440
taacagaaat tgttgattat agttaaatga gtcgttgata atttgtttaa atttgtaaac 1500
tcttcaggca aaatttatat tatgtgaaca aaattgccat atatttcata tcatgaatga 1560
atttggtcta aacatactcc atactgtcaa ggtttaaaaa acattatatt catacaatca 1620
taccatgaat gaatttgtgt aaagctgtaa actcttcttg catcctagct attttcctct 1680
tcttcttttt tctttttctg actagtcata tagatttttt ttttgatttt tatttgatcg 1740
ttggagaatc ttcaaaagat gactccactc atcttttcaa aaactaccta gaccatatac 1800
agggctctcc gtctacaaca attagcgtcc ggaaaacgta aagaagtcta caacaattag 1860
cgtcctcgtg gatcagtatc gtgacatttg tgtcatggaa cctatccttg gcagtcgaag 1920
ttaggctcac tcccaacggt ctaacttcta agtgctcact aaaatatgaa aaactaatgt 1980
tctcgaacaa acaccaccta taatgactca accctccata aaattcgaaa actgatctta 2040
aacaaaaata tatataagtg acgtaaaaca taaatttatg tatatctatt aaataataaa 2100
ttagtgaagt aaaacataaa cttaatcgca taaaagttta actttatttt gcttctcaat 2160
agattttgac tacataatag acgtggattt tagtcaaaca caagcgatat tgcaaaattt 2220
ccgatatata tatatacacg tctttttcac taatagaaaa gtaaacatat cagctcatct 2280
tttctctatt ggcagttctc atcatcttaa ttgaaaattt atgtttccac tttttttagc 2340
ttagatacaa actcaacttt gtaaaagatt ttcttgttgc atattaaatt ttacatcaca 2400
acaaaaagaa aaaaagaaat aaacaaagga gatcaaatag gttgtacata aattaagcaa 2460
tcatacaaca tcaaatcaac agctaaaaag ttcagtacaa tctttacttc cgaatttact 2520
tcattgtctt gacatcatgt tctaaacaaa aaccaaacca aagttctcca caaacctgtc 2580
ggtttttaac caaaccaaag ttactagctt aattcaaaaa aaaaaaaaaa cagaactgat 2640
tcagaaaccc caaattgaca aattgtcaaa ttgtcaaaaa gtggtcatct ttatcctttg 2700
acatctggtt agtataaaaa attgtaataa atgcaaacat aagatccaaa actacacctt 2760
attagaacgg aggaattaag agtgacataa ctcactccaa ctacgaccag tacaagtagt 2820
tggcgacatt aactacctct caccaaccac caaacccaat ccccacaata ttaccattac 2880
tctcatataa ctacacatat tcatatttac attttttgcc aacacaactc cttataagat 2940
atacacttca tcaacctata gatctcactc acataatcaa cctacaaaac aaaaacaaga 3000
<210> 79
<211> 3000
<212> DNA
<213> 拟南芥()
<220>
<221> 启动子
<222> (1)..(.3000)
<223> 极长链脂肪酸缩合酶 (CER60, KCS5), 蜡/角质启动子, pAtCER60,At1g25450
<400> 79
ggaaaacaaa aaaaagcttc aataaacata agccagttta ttttatcaaa aatgttatga 60
caaataatgt aaacatgaga tatgttttga ccagattgtt gttgtccaga cgaacagaaa 120
ctcgagatgc gatgtggaag actccatcaa caccgttgac tgcttcgtca aagcttccct 180
ctatcgttag atcagcttcg aagatcttca acctttcttt tgctcctttt aagtcccaca 240
agaaacccac cttttcttca tcttcttaat aacaaggaaa gaaaattaca gaatcattta 300
agaaaccaaa aaaaaaaact tgtggaagca aacctaagaa tctttggagg attctaggta 360
aagtacctga gtctctaaca gttgttctta cgtaatgacc aaattctaga agtgacttga 420
taacatgaga ggctataaag ctggttcctc cggtaaccaa gtactctgcc attttattag 480
gctcctctga ttttgtgtaa cattgacaaa ctagatcatg cgattagatg gtctaatttt 540
attttatttt gtgttagaag gatgttcaat cctggttgat ttagtttatg tttgactttt 600
ttttatgtag tctttgaata atagtagtta catctaactt tcataaagac ttggattagt 660
ataacctaat tataagcttt gggcttgaag caaatataat gaactgaatg tgggctttta 720
aagtatccaa agaggtttat gaaatgtgat atattttttt tggtcttcaa tctcttgaaa 780
attggttagc aatccattgg cagttgaagg ctctctcaga gaccttgact agaaggcctc 840
aaaggcaaag gaaggtggaa ggaaaaatca aaattcccgg agctgaaagt tgacacaaag 900
gaatcaacct agttctttct tgtcttagct tttttgaagg gaaatgaaaa ttgacacaaa 960
agaaggtgga aggaaaaata tgatgcttgt cctttattgg acatatcatt ttgcagaata 1020
tatattcttc ctagcatttt gcatgcattg tgtctaggtt gtcatgttgt ggctggttta 1080
ctaagttcct agtacaatga acaaagcgcc agagctgaaa gttgaaataa caatcgtcaa 1140
catcccaacc gccttaacat taaaaccaac caaaaaaaca aaaacacatc ccaaccgaaa 1200
gcagcaatcc aggaagatta gtttatgtaa atgacaaaca taaatatgaa accgtcacat 1260
agttgagagt cgccgtgaat atttactttt taaataaaaa gatatggtta gccgctggtt 1320
ggtctgacac cccatgtatc attatactca ttatccgtat gaaggaccat tttttcatat 1380
aaggatatac acgtttgtaa cgtatgcaga gccaacacca agtatataac ttgtactgtc 1440
gtctctgatt ctgttaaagc ggttaactag attattgact atgataattc aatgtaacac 1500
caatttatgc cccggacaaa aaaaaaatgg tgaaaccaaa atatttgatt tgcatcccag 1560
ttactatttc cctgcaatga tatgggggcg gcggtttgga gatatcgcca atgccacaat 1620
ttgtcgtaaa aacagttaaa cattttactc actagtcact ccccacaaaa aaaacgatac 1680
ttagttccac tctctactac tacaaaactt gtatacatta aaaaacgatt tttattgagt 1740
agacattaat gatcggctta taagtctaaa caagtaatgt gacagaattc acgtgaacaa 1800
atgctcgagt gggatgtttt tattgcttct caagttggtg atctttaata tcgccaagat 1860
taggttgtgc attaaatttg gattactttt tataaattaa atcaagatat ggatatgata 1920
aactttctct cttttctttt agttaacata ctttatagac tttatactac attagattaa 1980
ttgcagattt aaacaatgtt taaaattaca aaaacgttaa atgtcatatt ttccatataa 2040
attttgtttt acgtaaaagc aatttacatg aattttgata aaatcatgaa aaaacagaag 2100
cggtgtccaa gattggggat gaatgagaaa acaccgcact gatagtaact gaaaagaatg 2160
taaccagtct cacgatgggg atggcggaga gaaagagaga gacaagacag tagagaaaag 2220
aaggtacgac ggagagatga aggttggaca gagagaacaa aacgagaacg gcaatggccg 2280
ggaaaaaagg agagtaggga ggaaaaaaaa aagaattttg tttttttagc ttttgtaggg 2340
ttttttgtta gtcgtaaaat ataaattata atatcaaaga aaataatgag ttaattttaa 2400
agaaggttag atgatatttt tcacaagttt ttgttgatat agtaaaccca caattttatt 2460
ttgtcattca aagtaaattt tcaacaaaat atagctgttg ctggtcacat tttaacggga 2520
aaaaataatt cttgtacctg gatgtataac ctctgaacct tgtattacaa atttacaaaa 2580
cacattgatc tcaatcagac cgaacatgta tatcgagtca actgacaaat ctcttttcct 2640
actcttgtta catattcttt ctaggaaaaa aaaatgcttt caggctcaat gaaatgaatt 2700
aaaacagcaa aaggttggta aaagtctttt tccccataat catttgctcc ataattgacg 2760
tttgtctggc aacaactgga aacacagata tagaaaaaaa gattggacag taaatcataa 2820
aaaaaaaaaa gcaaaattaa atagaaagac gaagcgagag aaagagcgtt gagtggcgtg 2880
agagtttgag tccgaaaatt aactcctctc ccatctgcca actttcctct gtttctcctt 2940
tatatcccaa acctattctt cctccacacc tcatcatagg catagcaaaa ctcttccaaa 3000
<210> 80
<211> 352
<212> PRT
<213> 拟南芥()
<220>
<223> MYB结构域蛋白96 (Myb96), At5g62470
<400> 80
Met Gly Arg Pro Pro Cys Cys Glu Lys Ile Gly Val Lys Lys Gly Pro
1 5 10 15
Trp Thr Pro Glu Glu Asp Ile Ile Leu Val Ser Tyr Ile Gln Glu His
20 25 30
Gly Pro Gly Asn Trp Arg Ser Val Pro Thr His Thr Gly Leu Arg Arg
35 40 45
Cys Ser Lys Ser Cys Arg Leu Arg Trp Thr Asn Tyr Leu Arg Pro Gly
50 55 60
Ile Lys Arg Gly Asn Phe Thr Glu His Glu Glu Lys Thr Ile Val His
65 70 75 80
Leu Gln Ala Leu Leu Gly Asn Arg Trp Ala Ala Ile Ala Ser Tyr Leu
85 90 95
Pro Glu Arg Thr Asp Asn Asp Ile Lys Asn Tyr Trp Asn Thr His Leu
100 105 110
Lys Lys Lys Leu Lys Lys Ile Asn Glu Ser Gly Glu Glu Asp Asn Asp
115 120 125
Gly Val Ser Ser Ser Asn Thr Ser Ser Gln Lys Asn His Gln Ser Thr
130 135 140
Asn Lys Gly Gln Trp Glu Arg Arg Leu Gln Thr Asp Ile Asn Met Ala
145 150 155 160
Lys Gln Ala Leu Cys Glu Ala Leu Ser Leu Asp Lys Pro Ser Ser Thr
165 170 175
Leu Ser Ser Ser Ser Ser Leu Pro Thr Pro Val Ile Thr Gln Gln Asn
180 185 190
Ile Arg Asn Phe Ser Ser Ala Leu Leu Asp Arg Cys Tyr Asp Pro Ser
195 200 205
Ser Ser Ser Ser Ser Thr Thr Thr Thr Thr Thr Ser Asn Thr Thr Asn
210 215 220
Pro Tyr Pro Ser Gly Val Tyr Ala Ser Ser Ala Glu Asn Ile Ala Arg
225 230 235 240
Leu Leu Gln Asp Phe Met Lys Asp Thr Pro Lys Ala Leu Thr Leu Ser
245 250 255
Ser Ser Ser Pro Val Ser Glu Thr Gly Pro Leu Thr Ala Ala Val Ser
260 265 270
Glu Glu Gly Gly Glu Gly Phe Glu Gln Ser Phe Phe Ser Phe Asn Ser
275 280 285
Met Asp Glu Thr Gln Asn Leu Thr Gln Glu Thr Ser Phe Phe His Asp
290 295 300
Gln Val Ile Lys Pro Glu Ile Thr Met Asp Gln Asp His Gly Leu Ile
305 310 315 320
Ser Gln Gly Ser Leu Ser Leu Phe Glu Lys Trp Leu Phe Asp Glu Gln
325 330 335
Ser His Glu Met Val Gly Met Ala Leu Ala Gly Gln Glu Gly Met Phe
340 345 350
<210> 81
<211> 333
<212> PRT
<213> 拟南芥()
<220>
<223> MYB结构域蛋白94 (AtMyb94), At3g47600
<400> 81
Met Gly Arg Pro Pro Cys Cys Asp Lys Ile Gly Val Lys Lys Gly Pro
1 5 10 15
Trp Thr Pro Glu Glu Asp Ile Ile Leu Val Ser Tyr Ile Gln Glu His
20 25 30
Gly Pro Gly Asn Trp Arg Ser Val Pro Thr His Thr Gly Leu Arg Arg
35 40 45
Cys Ser Lys Ser Cys Arg Leu Arg Trp Thr Asn Tyr Leu Arg Pro Gly
50 55 60
Ile Lys Arg Gly Asn Phe Thr Glu His Glu Glu Lys Met Ile Leu His
65 70 75 80
Leu Gln Ala Leu Leu Gly Asn Arg Trp Ala Ala Ile Ala Ser Tyr Leu
85 90 95
Pro Glu Arg Thr Asp Asn Asp Ile Lys Asn Tyr Trp Asn Thr His Leu
100 105 110
Lys Lys Lys Leu Lys Lys Met Asn Asp Ser Cys Asp Ser Thr Ile Asn
115 120 125
Asn Gly Leu Asp Asn Lys Asp Phe Ser Ile Ser Asn Lys Asn Thr Thr
130 135 140
Ser His Gln Ser Ser Asn Ser Ser Lys Gly Gln Trp Glu Arg Arg Leu
145 150 155 160
Gln Thr Asp Ile Asn Met Ala Lys Gln Ala Leu Cys Asp Ala Leu Ser
165 170 175
Ile Asp Lys Pro Gln Asn Pro Thr Asn Phe Ser Ile Pro Asp Leu Gly
180 185 190
Tyr Gly Pro Ser Ser Ser Ser Ser Ser Thr Thr Thr Thr Thr Thr Thr
195 200 205
Thr Arg Asn Thr Asn Pro Tyr Pro Ser Gly Val Tyr Ala Ser Ser Ala
210 215 220
Glu Asn Ile Ala Arg Leu Leu Gln Asn Phe Met Lys Asp Thr Pro Lys
225 230 235 240
Thr Ser Val Pro Leu Pro Val Ala Ala Thr Glu Met Ala Ile Thr Thr
245 250 255
Ala Ala Ser Ser Pro Ser Thr Thr Glu Gly Asp Gly Glu Gly Ile Asp
260 265 270
His Ser Leu Phe Ser Phe Asn Ser Ile Asp Glu Ala Glu Glu Lys Pro
275 280 285
Lys Leu Ile Asp His Asp Ile Asn Gly Leu Ile Thr Gln Gly Ser Leu
290 295 300
Ser Leu Phe Glu Lys Trp Leu Phe Asp Glu Gln Ser His Asp Met Ile
305 310 315 320
Ile Asn Asn Met Ser Leu Glu Gly Gln Glu Val Leu Phe
325 330
<210> 82
<211> 355
<212> PRT
<213> 小盐芥()
<220>
<223> MYB结构域蛋白96 (ThMyb96)
<400> 82
Met Gly Arg Pro Pro Cys Cys Glu Lys Thr Gly Val Lys Lys Gly Pro
1 5 10 15
Trp Thr Pro Glu Glu Asp Ile Ile Leu Val Ser Tyr Ile Gln Glu His
20 25 30
Gly Pro Gly Asn Trp Arg Ser Val Pro Thr Asn Thr Gly Leu Lys Arg
35 40 45
Cys Ser Lys Ser Cys Arg Leu Arg Trp Thr Asn Tyr Leu Arg Pro Gly
50 55 60
Ile Lys Arg Gly Asn Phe Thr Glu His Glu Glu Lys Met Ile Val His
65 70 75 80
Leu Gln Ala Leu Leu Gly Asn Arg Trp Ala Ala Ile Ala Ser Tyr Leu
85 90 95
Pro Glu Arg Thr Asp Asn Asp Ile Lys Asn Tyr Trp Asn Thr His Leu
100 105 110
Lys Lys Lys Leu Lys Lys Ile Asn Glu Phe Gly Glu Glu Asp Asn Asp
115 120 125
Gly Phe Ser Ser Ser Asn Thr Ser Ser Gln Lys Gln His Gln Ser Ser
130 135 140
Asn Lys Gly Gln Trp Glu Arg Arg Leu Gln Thr Asp Ile Asn Met Ala
145 150 155 160
Lys Gln Ala Leu Cys Glu Ala Leu Ser Leu Asp Lys Pro Ser Ser Ser
165 170 175
Thr Leu Ser Pro Ser Ser Ser Pro Leu Ser Pro Val Ile Val Pro Gln
180 185 190
Asn Ile Pro Ser Phe Ser Ser Ala Leu Leu Asp Arg Cys Tyr Asp Leu
195 200 205
Ser Ser Ser Ser Ser Ser Thr Thr Thr Thr Thr Thr Thr Thr Ile Thr
210 215 220
Ser Asn Thr Thr Thr Asn Pro Tyr Pro Ser Gly Val Tyr Ala Ser Ser
225 230 235 240
Ala Glu Asn Ile Ala Arg Leu Leu Gln Asp Phe Met Lys Asp Thr Pro
245 250 255
Lys Ala Leu Thr Leu Thr Ser Ser Ser Pro Val Ser Glu Thr Gly Pro
260 265 270
Leu Ser Ala Ala Ala Cys Glu Glu Gly Gly Glu Gly Phe Glu Gln Ser
275 280 285
Phe Phe Ser Phe Asn Ser Met Glu Glu Thr Gln Asn Leu Thr Gln Glu
290 295 300
Thr Arg Phe Phe His Asp Gln Glu Ser Lys Pro Val Ile Ser Met Asp
305 310 315 320
Gln Asp His Gly Leu Ile Ser Gln Gly Ser Leu Ser Leu Leu Glu Lys
325 330 335
Trp Leu Phe Asp Glu Asn Met Val Gly Met Ala Leu Glu Gly Gln Glu
340 345 350
Ala Met Phe
355
<210> 83
<211> 307
<212> PRT
<213> 葡萄()
<220>
<223> MYB结构域蛋白30 (VvMyb30)
<400> 83
Met Gly Arg Pro Pro Cys Cys Asp Lys Ile Gly Val Lys Lys Gly Pro
1 5 10 15
Trp Thr Pro Glu Glu Asp Ile Ile Leu Val Ser Tyr Ile Gln Glu His
20 25 30
Gly Pro Gly Asn Trp Arg Ala Val Pro Thr Ser Thr Gly Leu Leu Arg
35 40 45
Cys Ser Lys Ser Cys Arg Leu Arg Trp Thr Asn Tyr Leu Arg Pro Gly
50 55 60
Ile Lys Arg Gly Asn Phe Thr Asp Gln Glu Glu Lys Thr Ile Ile His
65 70 75 80
Leu Gln Ala Leu Leu Gly Asn Arg Trp Ala Ala Ile Ala Ser Tyr Leu
85 90 95
Pro Gln Arg Thr Asp Asn Asp Ile Lys Asn Tyr Trp Asn Thr His Leu
100 105 110
Lys Lys Lys Leu Lys Lys Phe Pro Thr Gly Val Asp Asp His Asn Gln
115 120 125
Asp Gly Phe Ser Ile Ser Lys Gly Gln Trp Glu Arg Arg Leu Gln Thr
130 135 140
Asp Ile His Met Ala Lys Gln Ala Leu Cys Glu Ala Leu Ser Ile Asp
145 150 155 160
Thr Ser Ser Ser Leu Pro Asp Leu Lys Ser Ser Asn Gly Tyr Asn Pro
165 170 175
Asn Thr Arg Pro Val Gln Ala Ser Thr Tyr Ala Ser Ser Ala Glu Asn
180 185 190
Ile Ala Lys Leu Leu Glu Gly Trp Met Arg Asn Ser Pro Lys Ser Thr
195 200 205
Arg Thr Asn Ser Glu Ala Thr Gln Asn Ser Lys Asn Ser Ser Glu Gly
210 215 220
Ala Thr Thr Pro Asp Ala Leu Asp Ser Leu Phe Ser Phe Asn Ser Ser
225 230 235 240
Asn Ser Asp Leu Ser Leu Ser Asn Asp Glu Thr Ala Asn Phe Thr Pro
245 250 255
Glu Thr Ile Leu Phe Gln Asp Glu Ser Lys Pro Asn Leu Glu Thr Gln
260 265 270
Val Pro Leu Thr Met Ile Glu Lys Trp Leu Phe Asp Glu Gly Ala Ala
275 280 285
Thr Gln Glu Gln Glu Asp Leu Ile Asp Met Ser Leu Glu Asp Thr Ala
290 295 300
Gln Leu Phe
305
<210> 84
<211> 322
<212> PRT
<213> 毛果杨()
<220>
<223> MYB结构域蛋白081 (PtMyb081)
<400> 84
Met Gly Arg Pro Pro Cys Cys Asp Lys Ile Gly Val Lys Lys Gly Pro
1 5 10 15
Trp Thr Pro Glu Glu Asp Ile Ile Leu Val Ser Tyr Ile Gln Glu His
20 25 30
Gly Pro Gly Asn Trp Arg Ala Val Pro Thr Ser Thr Gly Leu Leu Arg
35 40 45
Cys Ser Lys Ser Cys Arg Leu Arg Trp Thr Asn Tyr Leu Arg Pro Gly
50 55 60
Ile Lys Arg Gly Asn Phe Thr Asp His Glu Glu Lys Met Ile Ile His
65 70 75 80
Leu Gln Ala Leu Leu Gly Asn Arg Trp Ala Ala Ile Ala Ser Tyr Leu
85 90 95
Pro Gln Arg Thr Asp Asn Asp Ile Lys Asn Phe Trp Asn Thr His Leu
100 105 110
Lys Lys Lys Leu Arg Lys Leu Gln Ala Gly Gln Glu Gly Gln Ser Arg
115 120 125
Asp Gly Leu Ser Ser Thr Gly Ser Gln Gln Ile Ser Arg Gly Gln Trp
130 135 140
Glu Arg Arg Leu Gln Thr Asp Ile Asn Met Ala Arg Gln Ala Leu Cys
145 150 155 160
Glu Ala Leu Ser Pro Gly Lys Pro Ser Ser Leu Leu Thr Gly Leu Lys
165 170 175
Pro Ser Cys Gly Tyr Glu Lys Pro Ala Thr Glu Pro Ile Tyr Ala Ser
180 185 190
Ser Thr Glu Asn Ile Ser Arg Leu Leu Lys Gly Trp Met Ile Ser Gly
195 200 205
Pro Lys Gln Ser Leu Lys Asn Ser Thr Thr Gln Asn Ser Phe Ile Asp
210 215 220
Thr Ala Gly Ala Asp Ser Leu Ser Ser Glu Gly Thr Pro Asp Lys Ala
225 230 235 240
Asp Lys Asn Gly Thr Gly Leu Ser Gln Ala Phe Glu Ser Leu Phe Gly
245 250 255
Phe Asp Ser Phe Asp Ser Ser Asn Ser Asp Phe Ser Gln Ser Met Ser
260 265 270
Pro Asp Thr Gly Leu Phe Gln Asp Glu Ser Lys Pro Asn Ser Ser Ala
275 280 285
Gln Val Pro Leu Ser Leu Ile Glu Arg Trp Leu Phe Asp Glu Gly Ala
290 295 300
Met Gln Gly Lys Asp Tyr Ile Asn Glu Val Thr Ile Asp Glu Asp Asn
305 310 315 320
Leu Phe
<210> 85
<211> 333
<212> PRT
<213> 蒺藜状苜蓿()
<220>
<223> MYB结构域蛋白(MtMyb), MTR4g108430
<400> 85
Met Gly Arg Pro Pro Cys Cys Glu Lys Leu Gly Ile Lys Lys Gly Pro
1 5 10 15
Trp Thr Pro Glu Glu Asp Ile Ile Leu Val Ser Tyr Ile Gln Gln His
20 25 30
Gly Pro Gly Asn Trp Arg Ser Val Pro Thr Asn Thr Gly Leu Met Arg
35 40 45
Cys Ser Lys Ser Cys Arg Leu Arg Trp Thr Asn Tyr Leu Arg Pro Gly
50 55 60
Ile Lys Arg Gly Asn Phe Thr Asp His Glu Glu Lys Met Ile Val His
65 70 75 80
Leu Gln Ala Leu Leu Gly Asn Arg Trp Ala Ala Ile Ala Ser Tyr Leu
85 90 95
Pro Gln Arg Thr Asp Asn Asp Ile Lys Asn Tyr Trp Asn Thr His Leu
100 105 110
Lys Arg Lys Met Asn Lys Asp Gln Ser Ser Thr Asp Glu Gly Val Asp
115 120 125
Gln Glu Ser Arg Ser Gln Leu Pro Asn Lys Gly Gln Trp Glu Arg Arg
130 135 140
Leu Gln Thr Asp Ile His Met Ala Lys Gln Ala Leu Ser Glu Ala Leu
145 150 155 160
Ser Leu Gln His Asn Pro Thr Thr Leu Gly Thr Leu Pro Asp Gln Met
165 170 175
Lys Pro Ser Ser Ser Phe Ser His Ser His Glu His Pro Pro Asn Leu
180 185 190
Asn Ile Pro Ser Pro Tyr Ala Ser Ser Tyr Glu Asn Ile Ser Arg Leu
195 200 205
Met Glu Thr Trp Met Lys Ser Pro Asn Ser Ser Ala Glu Thr Asn Ser
210 215 220
Ser Ser Ile Phe Ser Asn Met Gln Gly Ser Ser Cys Ser Glu Gly Ala
225 230 235 240
Gln Ser Thr Thr Gln Asp His His Gly Leu Asn Ser Ser Lys Ser Asp
245 250 255
Tyr Ala Ser Arg Phe Arg Ser Ser His Glu Gly Asn Asn Ser Phe Asn
260 265 270
Leu Asn Thr Lys Glu Gly Leu Phe Phe His Gln Glu Glu Arg Ile Asn
275 280 285
Ile Lys Ala Asn Met Glu Thr His Val Pro Leu Thr Leu Leu Glu Lys
290 295 300
Trp Leu Phe Glu Asp Gly Gly Ala Ser His Glu Cys His Glu Glu Leu
305 310 315 320
Ile Asn Met Ser Leu Glu Gly Thr Thr Ser Asp Phe Phe
325 330
<210> 86
<211> 310
<212> PRT
<213> 蒺藜状苜蓿()
<220>
<223> MYB结构域蛋白(MtMyb), MTR_3g039990
<400> 86
Met Val Arg Pro Pro Cys Cys Glu Lys Ile Gly Val Lys Lys Gly Pro
1 5 10 15
Trp Thr Pro Glu Glu Asp Ile Ile Leu Val Ser Tyr Ile Gln Glu His
20 25 30
Gly Pro Gly Asn Trp Arg Ser Val Pro Thr Thr Thr Gly Leu Met Arg
35 40 45
Cys Ser Lys Ser Cys Arg Leu Arg Trp Thr Asn Tyr Leu Arg Pro Gly
50 55 60
Ile Lys Arg Gly Asn Phe Asn Asp His Glu Glu Lys Met Ile Ile His
65 70 75 80
Leu Gln Ala Leu Leu Gly Asn Arg Trp Ala Ala Ile Ala Ser Tyr Leu
85 90 95
Pro Glu Arg Thr Asp Asn Asp Ile Lys Asn Tyr Trp Asn Thr His Leu
100 105 110
Arg Lys Lys Leu Lys Lys Ile Asn Gln Thr Gly Asp Glu Asn Glu Val
115 120 125
Glu Glu Asn Ser Ile Pro Gln Val Lys Gly Gln Trp Glu Arg Arg Leu
130 135 140
Gln Thr Asp Ile His Met Ala Lys Gln Ala Leu Cys Glu Ala Leu Ser
145 150 155 160
Leu Asp Lys Pro Thr Pro Ile Leu Ala Glu Asn Gln Thr Ser Pro Tyr
165 170 175
Ala Ser Asn Thr Glu Asn Ile Ala Arg Leu Leu Glu Lys Trp Met Lys
180 185 190
Lys Pro Glu Asn Ser Val Glu Thr Thr Asn Ser Gly Asn Ser Ile Met
195 200 205
Val Val Thr Gly Ser Gly Ser Arg Glu Gly Gly Gln Asn Thr Ile Ala
210 215 220
Cys Lys Gln Lys Asp Gln Ala Phe Asp Ser Leu Val Ser Phe Asn Ser
225 230 235 240
Leu Asn Ser Asp Cys Ser Gln Ser Val Ser Val Glu Glu Lys Asn Phe
245 250 255
Leu Ala Met Asp Ser Cys Phe Phe Gln Tyr Gln Ser Lys Pro Asn Gln
260 265 270
Glu Thr Gln Asp Pro Leu Met Phe Met Glu Asn Trp Pro Phe Asp Asp
275 280 285
Glu Ala Ala Gln Cys Asn Glu Asp Leu Met Asn Val Ser Met Glu Glu
290 295 300
Asn Thr Pro Gly Leu Phe
305 310
<210> 87
<211> 351
<212> PRT
<213> 大叶来檬()
<220>
<223> MYB结构域蛋白60 (CmMyb60)
<400> 87
Met Gly Arg Pro Pro Cys Cys Asp Lys Ile Gly Ile Lys Lys Gly Pro
1 5 10 15
Trp Thr Pro Glu Glu Asp Ile Ile Leu Val Ser Tyr Ile Gln Glu His
20 25 30
Gly Pro Gly Asn Trp Arg Ala Val Pro Thr Asn Thr Gly Leu Leu Arg
35 40 45
Cys Ser Lys Ser Cys Arg Leu Arg Trp Thr Asn Tyr Leu Arg Pro Gly
50 55 60
Ile Lys Arg Gly Asn Phe Thr Asp Gln Glu Glu Lys Met Ile Ile His
65 70 75 80
Leu Gln Ala Leu Leu Gly Asn Arg Trp Ala Ala Ile Ala Ser Tyr Leu
85 90 95
Pro Gln Arg Thr Asp Asn Asp Ile Lys Asn Tyr Trp Asn Thr His Leu
100 105 110
Lys Lys Lys Val Lys Lys Leu Gln Leu Ala Ala Ala Gly Cys Ser Glu
115 120 125
Asp Asn Ser Gln Tyr Arg Asp Glu Leu Ala Ser Ala Ser Ser Gln Gln
130 135 140
Ile Ser Arg Gly Gln Trp Glu Arg Arg Leu Gln Thr Asp Ile His Met
145 150 155 160
Ala Lys Gln Ala Leu Cys Ala Ala Leu Ser Pro Asp Lys Ala Ser Ile
165 170 175
Leu Ser Glu Leu Lys Pro Ala Asn Gly Phe Ile Ser Tyr Thr Lys Pro
180 185 190
Ala Val Gln Ala Pro Thr Tyr Ala Ser Ser Thr Glu Asn Ile Ala Lys
195 200 205
Leu Leu Lys Gly Trp Ala Arg Asn Ala Gln Lys Ser Ala Ser Ser Asn
210 215 220
Ser Gly Val Thr Asp Gln Asn Ser Ile Asn Asn Asn Val Asn His Ile
225 230 235 240
Ala Gly Ala Glu Ser Ala Ser Ser Glu Glu Thr Pro Ser Lys Val Ala
245 250 255
Ser Asn Ser Thr Gly Ile Glu Leu Ser Glu Ala Phe Glu Ser Leu Phe
260 265 270
Gly Phe Glu Ser Phe Asp Ser Ser Asn Ser Thr Asp Leu Ser Gln Ser
275 280 285
Val Thr Pro Glu Ser Ser Thr Phe Gln Asp Tyr Glu Ser Lys Gln Leu
290 295 300
Leu Leu Asp Pro Ser Ala Ser Ala Asp Asp Asp Gln Met Pro Gln Leu
305 310 315 320
Ser Leu Ile Glu Lys Trp Leu Phe Asp Asp Gln Gly Ala Lys Asp Tyr
325 330 335
Leu Asn Asp Leu Lys Leu Asp Asp His Glu Asp Thr Asp Met Phe
340 345 350
<210> 88
<211> 303
<212> PRT
<213> 紫短柄草()
<220>
<223> MYB结构域蛋白(BdMyb)
<400> 88
Met Gly Arg Pro Pro Cys Cys Asp Gln Ala Gly Val Lys Lys Gly Pro
1 5 10 15
Trp Thr Pro Glu Glu Asp Leu Met Leu Val Ser Tyr Ile Gln Glu His
20 25 30
Gly Pro Gly Asn Trp Arg Ala Val Pro Thr Asn Thr Gly Leu Met Arg
35 40 45
Cys Ser Lys Ser Cys Arg Leu Arg Trp Thr Asn Tyr Leu Arg Pro Gly
50 55 60
Ile Lys Arg Gly Asn Phe Thr Asp Gln Glu Glu Lys Leu Ile Val His
65 70 75 80
Leu Gln Ala Leu Leu Gly Asn Arg Trp Ala Ala Ile Ala Ser Tyr Leu
85 90 95
Pro Glu Arg Thr Asp Asn Asp Ile Lys Asn Tyr Trp Asn Thr His Leu
100 105 110
Lys Arg Lys Leu Lys Lys Met Ser Ala Ala Ala Gly Glu Asp Gly Ala
115 120 125
Ala Ala Ala Thr Ala Gly Gly Ala Glu Ala Lys Ser Arg Ala Thr Ala
130 135 140
Pro Lys Gly Gln Trp Glu Arg Arg Leu Gln Thr Asp Ile His Thr Ala
145 150 155 160
Arg Gln Ala Leu Arg Asp Ala Leu Ser Leu Asp Thr Thr Ala Pro Ala
165 170 175
Pro Pro Lys Pro Ala Pro Met Glu Arg Ser Ser Lys Gly Ala Val Tyr
180 185 190
Ala Ser Ser Ala Glu Asn Ile Ala Arg Leu Leu Glu Gly Trp Met Arg
195 200 205
Pro Gly Glu Gly Lys Ala Ser Ser Gly Gly Ser Gly Ser Gly Ser Arg
210 215 220
Ser Ser Ala Ser Val Val Ser Ala Glu Gly Ala Ser Ala Ser His Ser
225 230 235 240
Gly Thr Ala Pro Thr Pro Glu Gly Ser Thr Val Thr Ser Lys Thr Lys
245 250 255
Asp Glu Val Ala Val Ala Ala Pro Pro Ala Phe Ser Met Leu Glu Asn
260 265 270
Trp Leu Phe Asp Asp Gly Met Gly Met Gly His Asn Gly Ile Gly Asp
275 280 285
Val Gly Leu Asp Asp Val Pro Leu Gly Asp Pro Ser Glu Phe Phe
290 295 300
<210> 89
<211> 269
<212> PRT
<213> 紫短柄草()
<220>
<223> MYB结构域蛋白(BdMyb)
<400> 89
Met Gly Arg Pro Pro Cys Cys Asp Lys Asp Gly Val Lys Lys Gly Pro
1 5 10 15
Trp Thr Pro Glu Glu Asp Ile Ile Leu Val Ser Tyr Val Gln Asp His
20 25 30
Gly Pro Gly Asn Trp Arg Ala Val Pro Pro Asn Thr Gly Leu Met Arg
35 40 45
Cys Ser Lys Ser Cys Arg Leu Arg Trp Thr Asn Tyr Leu Arg Pro Gly
50 55 60
Ile Arg Arg Gly Asn Phe Ser Glu Gln Glu Glu Lys His Ile Val Gln
65 70 75 80
Leu Gln Ala Leu Leu Gly Asn Arg Trp Ala Ala Ile Ala Ser Tyr Leu
85 90 95
Pro Asp Arg Thr Asp Asn Asp Ile Lys Asn Tyr Trp Asn Thr His Leu
100 105 110
Lys Lys Lys Leu Leu His Arg Thr Ser Thr Ala Thr Pro Ala Pro Ala
115 120 125
Pro Thr Thr His Lys Asp Gln Asn Asn Asn Lys Gly Gln Trp Glu Arg
130 135 140
Arg Leu Gln Thr Asp Ile His Leu Ala Arg Gln Ala Leu Arg Glu Ala
145 150 155 160
Leu Ser Leu Asp Thr Ala Ser Thr Ser Ala Thr Pro Gly Pro Ala Ala
165 170 175
Tyr Ala Leu Ser Ala Gln Asn Val Ser Arg Met Leu Asp Asp Trp Ala
180 185 190
Val Ala Ala Asp Ser Ala Ser Ser Glu Val Thr Glu Cys Ser Gly Gly
195 200 205
Ser Thr Ala Ser Asn Gly Thr Leu Trp Ser Ser Leu Leu Gly Arg Glu
210 215 220
Ser Thr Gly Ala Ala Ala Ala Gly Val Glu Asp Pro Ala Ala Leu Ser
225 230 235 240
Ala Ile Glu Ser Trp Leu Leu Leu Asp Asp Gly Thr Asp Arg Gln Gln
245 250 255
Pro Pro Glu Gln Glu Gln Ser Gly Gly Gln Leu Leu Pro
260 265
<210> 90
<211> 314
<212> PRT
<213> 小麦()
<220>
<223> MYB结构域蛋白(TaMyb)
<400> 90
Met Gly Arg Pro Pro Cys Cys Asp Lys Glu Gly Val Lys Lys Gly Pro
1 5 10 15
Trp Thr Pro Glu Glu Asp Leu Val Leu Val Ser Tyr Val Gln Glu His
20 25 30
Gly Pro Gly Asn Trp Arg Ala Val Pro Thr Arg Thr Gly Leu Met Arg
35 40 45
Cys Ser Lys Ser Cys Arg Leu Arg Trp Thr Asn Tyr Leu Arg Pro Gly
50 55 60
Ile Lys Arg Gly Asn Phe Thr Asp Gln Glu Glu Lys Leu Ile Val His
65 70 75 80
Leu Gln Ala Leu Leu Gly Asn Arg Trp Ala Ala Ile Ala Ser Tyr Leu
85 90 95
Pro Glu Arg Thr Asp Asn Asp Ile Lys Asn Tyr Trp Asn Thr His Leu
100 105 110
Lys Arg Asn Leu Gln Ala Gly Gly Asp Ala Ala Ala Lys Pro Ala Ala
115 120 125
Gln Arg Pro Ala Ser Ser Ser Lys Gly Gln Trp Glu Arg Arg Leu Gln
130 135 140
Thr Asp Ile Asn Met Ala Arg Arg Ala Leu Arg Glu Ala Leu Thr Thr
145 150 155 160
Leu Asp Asp Ile Lys Arg Gln Gln Pro Asp Ala Ala Asp Gly Val Asn
165 170 175
Gly Pro Ala Ala Ala Gly Ala Asp Ser Gly Ser Pro Ala Ala Ser Ser
180 185 190
Ser Ser Ala Ala Ser Leu Ser Gln Cys Ser Pro Ser Ala Ala Gly Pro
195 200 205
Tyr Val Leu Thr Thr Ala Asn Ile Ser Arg Met Leu Asp Gly Trp Ala
210 215 220
Ser Lys Gly Arg Ser Ala Val Pro Ala Ala Asp Ser Pro Ser Gly Ser
225 230 235 240
Ser Ala Ser Glu Val Ser Tyr Gly Ser Gly Ala Ala Ala Arg Ala Leu
245 250 255
Gly Ser Ala Phe Glu Tyr Asp Arg Lys Pro Ala Val Leu Ala Pro Asp
260 265 270
Gln Thr Gln Leu Asn Ala Ile Glu Thr Trp Leu Phe Ala Asp Asp Asn
275 280 285
Ser Asn Asn Asp His His Gly His Gly Gly Gly Gly Ser Gly Leu Leu
290 295 300
Gly Val Pro Ala Thr Leu Gly Tyr Pro Phe
305 310
<210> 91
<211> 321
<212> PRT
<213> 水稻()
<220>
<223> MYB结构域蛋白(OsMyb), Os09g0414300
<400> 91
Met Gly Arg Pro Pro Cys Cys Asp Lys Val Gly Val Lys Lys Gly Pro
1 5 10 15
Trp Thr Pro Glu Glu Asp Leu Met Leu Val Ser Tyr Ile Gln Glu His
20 25 30
Gly Ala Gly Asn Trp Arg Ala Val Pro Thr Asn Thr Gly Leu Met Arg
35 40 45
Cys Ser Lys Ser Cys Arg Leu Arg Trp Thr Asn Tyr Leu Arg Pro Gly
50 55 60
Ile Lys Arg Gly Asn Phe Thr Glu Gln Glu Glu Lys Leu Ile Val His
65 70 75 80
Leu Gln Ala Leu Leu Gly Asn Arg Trp Ala Ala Ile Ala Ser Tyr Leu
85 90 95
Pro Glu Arg Thr Asp Asn Asp Ile Lys Asn Tyr Trp Asn Thr His Leu
100 105 110
Lys Lys Lys Leu Lys Lys Met Gln Ala Ala Gly Gly Gly Glu Asp Ser
115 120 125
Gly Ala Ala Ser Glu Gly Gly Gly Gly Arg Gly Asp Gly Asp Gly Gly
130 135 140
Gly Lys Ser Val Lys Ala Ala Ala Pro Lys Gly Gln Trp Glu Arg Arg
145 150 155 160
Leu Gln Thr Asp Ile His Thr Ala Arg Gln Ala Leu Arg Asp Ala Leu
165 170 175
Ser Leu Asp His Pro Asp Pro Ser Pro Ala Thr Ala Ala Ala Ala Ala
180 185 190
Thr Pro Ala Gly Ser Ser Ala Ala Tyr Ala Ser Ser Ala Asp Asn Ile
195 200 205
Ala Arg Leu Leu Gln Gly Trp Met Arg Pro Gly Gly Gly Gly Gly Gly
210 215 220
Asn Gly Lys Gly Pro Glu Ala Ser Gly Ser Thr Ser Thr Thr Ala Thr
225 230 235 240
Thr Gln Gln Gln Pro Gln Cys Ser Gly Glu Gly Ala Ala Ser Ala Ser
245 250 255
Ala Ser Ala Ser Gln Ser Gly Ala Ala Ala Ala Ala Thr Ala Gln Thr
260 265 270
Pro Glu Cys Ser Thr Glu Thr Ser Lys Met Ala Thr Gly Gly Gly Ala
275 280 285
Gly Gly Pro Ala Pro Ala Phe Ser Met Leu Glu Ser Trp Leu Leu Asp
290 295 300
Asp Gly Gly Met Gly Leu Met Asp Val Val Pro Leu Gly Asp Pro Ser
305 310 315 320
Phe
<210> 92
<211> 310
<212> PRT
<213> 水稻()
<220>
<223> MYB结构域蛋白(OsMyb), Os08g0437200
<400> 92
Met Gly Arg Pro Pro Cys Cys Val Lys Ala Glu Val Lys Lys Gly Pro
1 5 10 15
Trp Thr Pro Glu Glu Asp Leu Met Leu Val Ala Tyr Val Gln Glu His
20 25 30
Gly Pro Gly Asn Trp Arg Ala Val Pro Thr Asn Thr Gly Leu Met Arg
35 40 45
Cys Ser Lys Ser Cys Arg Leu Arg Trp Thr Asn Tyr Leu Arg Pro Gly
50 55 60
Ile Lys Arg Gly Asn Phe Thr Asp Gln Glu Glu Lys Leu Ile Val His
65 70 75 80
Leu Gln Ala Leu Leu Gly Asn Arg Trp Ala Ala Ile Ala Ser Tyr Leu
85 90 95
Pro Glu Arg Thr Asp Asn Asp Ile Lys Asn Tyr Trp Asn Thr His Leu
100 105 110
Lys Lys Lys Leu Lys Lys Met Ser Ala Thr Gly Gly Gly Gly Asp Asp
115 120 125
Gly Glu Gly Gly Gly Ala Gly Glu Val Lys Thr Arg Ala Ala Ala Pro
130 135 140
Lys Gly Gln Trp Glu Arg Arg Leu Gln Thr Asp Ile His Thr Ala Arg
145 150 155 160
Gln Ala Leu Arg Asp Ala Leu Ser Leu Asp Pro Ser Pro Pro Ala Lys
165 170 175
Pro Leu Asp Ser Ser Ser Gly Ala Thr Ala Pro Pro Ser Ser Gln Ala
180 185 190
Ala Thr Ser Tyr Ala Ser Ser Ala Glu Asn Ile Ala Arg Leu Leu Glu
195 200 205
Gly Trp Met Arg Pro Gly Gly Gly Gly Gly Lys Thr Thr Thr Thr Pro
210 215 220
Ser Ser Gly Ser Arg Ser Ser Ala Ala Ser Val Leu Ser Gly Glu Ala
225 230 235 240
Ser His Ser Gly Gly Ala Thr Ala Pro Thr Pro Asp Gly Ser Thr Val
245 250 255
Thr Ser Lys Thr Lys Asp Glu Glu Thr Ala Gly Ala Pro Pro Pro Pro
260 265 270
Pro Pro Pro Ala Phe Ser Met Leu Glu Ser Trp Leu Leu Asp Asp Gly
275 280 285
Met Gly His Gly Glu Val Gly Leu Met Asp Val Val Val Pro Leu Gly
290 295 300
Asp Pro Ser Glu Phe Phe
305 310
<210> 93
<211> 296
<212> PRT
<213> 玉米()
<220>
<223> MYB结构域蛋白(ZmMyb)
<400> 93
Met Gly Arg Pro Pro Cys Cys Glu Lys Ala Gly Leu Lys Lys Gly Pro
1 5 10 15
Trp Ser Pro Glu Glu Asp Leu Leu Leu Val Ser Tyr Val Gln Glu His
20 25 30
Gly Pro Gly Asn Trp Arg Ala Val Pro Cys Ser Thr Gly Leu Met Arg
35 40 45
Cys Ser Lys Ser Cys Arg Leu Arg Trp Thr Asn Tyr Leu Arg Pro Gly
50 55 60
Ile Lys Arg Gly Ser Phe Ser Asp Gln Glu Glu Lys Leu Ile Ile His
65 70 75 80
Leu Gln Glu Leu Leu Gly Asn Lys Trp Ser Ala Ile Ala Ser Tyr Leu
85 90 95
Pro Glu Arg Thr Asp Asn Asp Ile Lys Asn Tyr Trp Asn Thr His Leu
100 105 110
Lys Lys Lys Leu Ala Lys Thr Gly Ala Arg Glu Ser Gly Ala Ser Ala
115 120 125
Lys Thr Thr Lys Lys Ser Asp Arg Ala Ala Ala Pro Lys Gly Gln Trp
130 135 140
Glu Arg Arg Leu Gln Thr Asn Ile His Thr Ala Arg Gln Ala Leu Arg
145 150 155 160
Glu Ala Leu Ser Met Asp Asp Thr Ala Pro Pro Ala Ile Lys Pro Glu
165 170 175
Pro Leu Pro Leu Pro Leu Gly Gln Leu Pro Ala Pro Ala Ser Gln Ala
180 185 190
Met Tyr Ala Ser Ser Ile Asp Asn Ile Ala Arg Leu Leu Glu Gly Trp
195 200 205
Met Arg Pro Ser Val Ser Gly Asn Ala Ser Ala Glu Ser Met Ser Ser
210 215 220
Phe Ser Ala Phe Ser Gly Gly Gly Asp Gly Ala Ser Ala Ser His Ile
225 230 235 240
Gly Thr Ala His Thr Pro Glu Gly Phe Thr Gly Thr Arg Lys Glu Glu
245 250 255
Gly Ala Gly Pro Gly Pro Ala Ser Leu Pro Met Phe Glu Asn Trp Leu
260 265 270
Leu Asp Asp Gly Met Gly Asn Gly Asp Ala Ser Leu Ile Cys Val Pro
275 280 285
Leu Ala Asp Pro Cys Glu Phe Phe
290 295
<210> 94
<211> 3000
<212> DNA
<213> 拟南芥()
<220>
<221> 启动子
<222> (1)..(.3000)
<223> 导管特异性的REF4 启动子, At2g48110
<400> 94
tttcgcaggc cctaattaag acattcaaga aaacaagaag aagcataaga agaggcctaa 60
atgcccagag aattaaacaa atgggccttt aacaatattt taaacaacac tgaacaatag 120
atgagatctc aacttcgaaa agctaaagcc ttaatataaa accaaacatc aatctctcgc 180
cgttaaggtt acatctgtcg ccgccgctga gaccgccact taaggccgtc tcctccgctc 240
tatccatatc caaaacagct ataaagataa acttccagag cttggtgaag gagcagcaac 300
cctagttttc aatccctaaa ggtgaagttt ttgttcttct ttttctttga ttctactctt 360
ttttcgtcac aatagcgcag tagcgatctg ggttggcgtt tttattgacg gattttggtg 420
ggtttgggtt cgtgttagat tttactttca cagattcagg tatgtatttg atcttattta 480
gctttggtaa tactcaaacc aactagtatt tgatcttaat taggtgacgt tgatattttg 540
tgtgttgtgg gtcaatcatc cactctaggt tgatctgcgt ctatgtttag tcctaatttt 600
gaagaatcat tctaattgct gatgtttatt tatgacaatt ttatgtctta cttcgaattt 660
agctaagata ataaatctag acgagtgcct ttataaacag tttatgtatg aacaatgctt 720
cgcttgtggg attgttgatt tcactacatt tgattaagaa tgtacatact attacaaatg 780
ttatcgatta aaaacgtcat tttcagatat tagttctcca gcgagttgac acaaaacccg 840
attacgtttc atccggcgac tcgctttgat attccatgga ttctcagttg aatccttcca 900
agagacgcaa gataaggttc gcgtctctca tcttttcccc agtttttttt cttattcgaa 960
tcacttaagg cttgagaaat tagtggttag ggtgtgtaaa ttgcgtttga tatagtgtca 1020
ggcacagatg cgttgcgtgc tataagatgt tcaatagacg agaacacctc gttgagcaca 1080
tgaagatttc ctaccactca cttcaccagc ctcgctgtgg ggtttgcctc aagcactgta 1140
aatccttcga atccgtgagg gaacacctta acggtatgtt tgtatttgta ttcgttttcc 1200
accacacatc gtatgaatat ggtggttgtt gatgtttgtt tgattccgtc tttccaacta 1260
tttcagttcc agaccatctt tccaaaggaa actgcaaagc cattttcact aaacgaggct 1320
gtactctctg tcttcaaatc tttgaggagg cctttgctct cgccgagcat aaaaacaagt 1380
gtcacctctc cccacctcgt cctcttgtaa gttttgttgg gaattattta gataatgtgg 1440
actatatatg ctctgccgcc tccaatattc ccgagtctat tctttctgat tattgaaata 1500
tcagcagttt tccccttaaa tgatctgatt agtgctttat tcatatcagg gaacatctac 1560
ccaaaggaat ccttctagtt cacttgctgg ttcacgtctc aaggctatgg cacttgactg 1620
tgaaatggtt ggtggtggtg ctgatgggac tattgatcag tgcgcatcgg tttgcctggt 1680
tgacgatgac gagaatgtga tcttctccac tcacgttcaa ccactgctcc ctgtcaccga 1740
ttacaggttc tgcttgtgga ccatttgtgc ttgttttgtt ttataattct tccttttaaa 1800
tctcaccccg cctcatgttc aggcacgaga taactggatt gactaaggaa gatttgaagg 1860
atggtatgcc acttgagcat gtacgagaga gagttttttc gttcttgtgc ggtggacaga 1920
atgatggggc tggaaggctt cttcttgttg gtcatgacct taggcatgat atgagttgct 1980
tgaagcttga ataccctagc catttgttga ggtaactaac tgacccgttt ttgttgactc 2040
tttgcttgaa atctaatgta attgctatgc ttcacctcag agacacagca aaatacgtgc 2100
cgttgatgaa gacaaatctt gtaagccaat cgctcaagta cctcacaaag tcatatctcg 2160
ggtaagttat gcttgggctt tgatttgttg acattggatt atgaaaactt ggaacatgag 2220
atagaaactg gttttgtttg tatgtgtaga tacaagatcc agtgcgggaa gcacgaggtt 2280
tacgaggact gtgtatctgc gatgagactg tacaagagaa tgcgggatca agagcatgtt 2340
tgtagtggaa aggcagaagg gaatggtctg aactcgcgga aacagagcga tctagagaag 2400
atgaatgcgg aagagctgta ccaaaaatca acgtcagagt accggtgctg gtgccttgac 2460
cgactcagca atccatgaaa tgaaatccca atttctcttt taaaagaaag ctccttctct 2520
tcctccctta agtctctttt tgttcgatgg gaggattaag aagacttgtt aagagctttt 2580
ccggctagtt tttggataat caaatatgat atggcttcat tcaacaacaa ctctcccttt 2640
gggaactcgt ataaaaagtt actacttaag cttcaaatct gtgtaactaa aatggataaa 2700
gtctgatgca tctgaggtct tggaatctgt tgctcatagt tgtggatacc agcaacatca 2760
aacattatta cttgtttacc aaacccactc tcagcttcca tttcatcagt ttgaagtcaa 2820
gtggataaaa agactgtcta cataactcaa atactgtagt attatattca catataaatg 2880
agaaattgac atttcctcta gaaaaagaaa agcaatcgtg tgagaagaaa caagtaaaag 2940
gctgaggaag aagacgacta ttagtccccg tcgaacttgt ttctcccggt cgtccaacaa 3000
<210> 95
<211> 3000
<212> DNA
<213> 拟南芥()
<220>
<221> 启动子
<222> (1)..(.3000)
<223> 导管特异性的RFR1 启动子, At3g23590
<400> 95
aaatgatttg ttttgtgaat agttgattcc agctaatgtg gtgaaagaca atcatcttac 60
gtttggtgat tgtatcaact acattgatga agaagggaac ttagtaggac aaagagggaa 120
taagcagatg ataggatttg cttcaaagga tgcatggcga cataatttgc agaaggatta 180
gacataataa catgaagata ctgtcaagta tgagctcctt catgaactta agtacgttca 240
taattccact tgatccacaa ctttgggcta ttggtataaa aagctaactt tatctagatt 300
acatgctaca tctcaattca agatgtccat ttattaaagc aaaggcatga ctttatcttc 360
gactagaaac tggtgatgga ccatcctcaa cctccactag catcatctcc acctactcct 420
aaccactaac ccaattatcc ttttcacctt ttcctctcaa cactatccta tttttaggtg 480
atttagggaa aagagaaata ctacactttg atgtaatttt ttttttaatt gaattgagct 540
ttgaatacta tggttaatat ggatgaaaga aaacacacgt tcctactaat ttctttttct 600
aaatgatgac gttttctcaa gttacttatc gtagtctaat cggttattga caaaaatgat 660
ctagacgttt gaaaatcatt ggccaacctc ttcactagat agaattttta tttctaacca 720
ttacctacct gatacacaca ccaagttctg aatctagaga tgatcttgtc tcatttgttt 780
atagactaaa gagtatataa aagctggatt catgtgggtc atccatcatt aatcgtgttc 840
ttcaattcac caacttcgta ggttccgcat cttttgtact ttctttctat ttttaatctt 900
gcaatatcaa aaataaatat atacaccttt taaaaaaatt ctgaatatgc atatggtaat 960
ggtatcatca gaaaacattg aatattgaat cgtctatcta ccaatctctt caagttgtga 1020
attttattta tcaaaaaaaa aaaaaaaatc aactgaaacg tcaatgttat gttttgcttc 1080
aatctaaaga aagttgtacc aaaaataaag tactttttta ccaagaaatt tcggattgta 1140
gccaaaatat gtcttgtttt attttttttg ttcaccaaaa atatgttaaa ctagtttttt 1200
gactttttcc cccatgcgtt gttgttgtta ttcggacgac caatatttaa tacagtattc 1260
aagtgtagct aagctactga ttccagataa agtaaaaaaa tgtctttgat accaacttca 1320
ctttgaccaa ttatcgaact ctttaatttc ataatatata gctttgacaa atatcccatc 1380
agaattcata aatcgccaca tgaatcttat aattgtcaac tatgtgcagt ccaatattaa 1440
tatcatggaa aactagatta ctagcattta atttcttaat tgtatttagg ttatagactt 1500
aacatggttt ttcttctaga gcattttcac tacatttttt tctttctatg ttattatttt 1560
atgttttact tattgttaca tccatttaca acgatgttgg ataaaatatt tttaactgtt 1620
cagtccgtaa atgactagct aaatttttac ggatatattt ttacataaaa aaagaaatta 1680
tttttacgat ttaccaattt aactcttctt tctctctaag ttttcattta ttaaaaatga 1740
tagttttttt aagaaagtta tttgtattta tttatttaaa attggatgca aaaaattaca 1800
aaacttggaa gaaatattgt taacccatct ttatcagtat agaatttctg attactccct 1860
gcgttgaaaa taagaagatc catagaacgt ggtaatgttt tttttttttt ttttctggca 1920
agaaatggta atgttaatca attagactca tgactaaagc tggaaaattt gactaaaatg 1980
gtattatctt gcattattac ctaaaagctc ttcttgcatt actctttcat gcaaaattgc 2040
ctccaaaagt catacttcga aatcatggct ttgtaactca cccttgccac catctatcta 2100
aacttattat tgtttaaaac aaaattgatt ttcaaagaaa aaaaaaatat aaactcccac 2160
cggtcaataa cttttaattt tcacttcaat tattcataaa ataaatatat acaccttgat 2220
tctttcttaa taactggagt tattttgaat ttaaattact tttgaaatac aaaagaaagt 2280
aagataattt tgccatttaa aatgtctaca acaattatgt taacacttaa catacatttt 2340
tagaaccaaa tggtaatggt ccactagtta aaagatttct tatgaatgtc tttttatgca 2400
gagttcgaat tttctcacaa cgattttaac cattttcgtc actcgcaaaa tttttagtgg 2460
ataacaaaaa aaaaaaaaag ttgaaattta atggaaattt aggagatttg tccaattttt 2520
aattttcccg attaatttaa aaaatctact ttacgccttt tattgacaaa gatgcatatt 2580
aactatatat atatatatat atatatatat atatatatat atatattata aactaaattt 2640
tgtagaaccc atcagaagaa atttgtactt ttaactttaa aatcaattaa aaaatttcaa 2700
ttcaaataaa aaaaaatcca aaattgttta taaattcaag aaatataaca cacaagttga 2760
cataaacagt ggagagattt gtgaaatgtg aactcttttg acgtctttct catatttcgt 2820
ctcgggacaa ttcacacgca ctgaatcatc tctctcacga ggactattca gccatttgca 2880
aacgcacaca caaacgcaca cgcgtttatt tttttttcgc caaatcaaat ctgaagagtt 2940
cctgtatctt ttaaccgctc ttcttcttct tcagagagct tcgttgattg aacggaaaaa 3000
<210> 96
<211> 711
<212> PRT
<213> 人工序列()
<220>
<223> 合成的苯丙氨酸氨裂合酶 (PAL) 共有序列（大多数）
<220>
<221> 变体
<222> (2)..(.2)
<223> Xaa = Glu, Ala, Val, Ser, Pro或Asp
<220>
<221> 变体
<222> (7)..(.7)
<223> Xaa = His, Asn, Met, Lys, Ala, Gly或Ser
<220>
<221> 变体
<222> (14)..(.14)
<223> Xaa = Val, Ser, Gly, Ala, Glu或Leu
<220>
<221> 变体
<222> (15)..(.15)
<223> Xaa = Met, Leu, Ser, Cys, Asn, Glu, Val, Phe或Ala
<220>
<221> 变体
<222> (17)..(.17)
<223> Xaa = Cys, Thr, Ala, Asn, Val, Trp或Ile
<220>
<221> 变体
<222> (19)..(.19)
<223> Xaa = Ala, Gln, Arg, Glu, Gly, Ile, Val, Thr或Ser
<220>
<221> 变体
<222> (31)..(.31)
<223> Xaa = Gln, Ala, Glu, Asp, Ser或Thr
<220>
<221> 变体
<222> (70)..(.70)
<223> Xaa = Ile, His, Ala, Arg, Gly, Leu, Ser或Asn
<220>
<221> 变体
<222> (143)..(.143)
<223> Xaa = Ser, Cys, Gly, Glu, Asn或Thr
<220>
<221> 变体
<222> (440)..(.440)
<223> Xaa = Ala或Gly
<220>
<221> 变体
<222> (594)..(.594)
<223> Xaa = Asp或Glu
<220>
<221> 变体
<222> (624)..(.624)
<223> Xaa = Val, Leu, Ala, Gln或Ile
<220>
<221> 变体
<222> (631)..(.631)
<223> Xaa = Ala, Ser, Leu或Gly
<220>
<221> 变体
<222> (640)..(.640)
<223> Xaa = Thr, Asn, Gln或Ser
<220>
<221> UNSURE
<222> (2)..(2)
<223> The 'Xaa' at location 2 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (7)..(7)
<223> The 'Xaa' at location 7 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (14)..(14)
<223> The 'Xaa' at location 14 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (15)..(15)
<223> The 'Xaa' at location 15 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (17)..(17)
<223> The 'Xaa' at location 17 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (19)..(19)
<223> The 'Xaa' at location 19 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (31)..(31)
<223> The 'Xaa' at location 31 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (70)..(70)
<223> The 'Xaa' at location 70 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (143)..(143)
<223> The 'Xaa' at location 143 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (440)..(440)
<223> The 'Xaa' at location 440 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (594)..(594)
<223> The 'Xaa' at location 594 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (624)..(624)
<223> The 'Xaa' at location 624 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (631)..(631)
<223> The 'Xaa' at location 631 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (640)..(640)
<223> The 'Xaa' at location 640 stands for Gln, Arg, Pro, or Leu.
<400> 96
Met Xaa Ala Glu Ala Gly Xaa Gly His Ala Asn Gly Ser Xaa Xaa Cys
1 5 10 15
Xaa Ser Xaa Ala Asp Pro Leu Asn Trp Gly Val Ala Ala Glu Xaa Met
20 25 30
Lys Gly Ser His Leu Asp Glu Val Lys Arg Met Val Ala Glu Tyr Arg
35 40 45
Lys Pro Val Val Lys Leu Gly Gly Glu Thr Leu Thr Ile Ala Gln Val
50 55 60
Ala Ala Ile Ala Ala Xaa Asp Asp Gly Val Lys Val Glu Leu Ser Glu
65 70 75 80
Ser Ala Arg Ala Gly Val Lys Ala Ser Ser Asp Trp Val Met Asp Ser
85 90 95
Met Asn Lys Gly Thr Asp Ser Tyr Gly Val Thr Thr Gly Phe Gly Ala
100 105 110
Thr Ser His Arg Arg Thr Lys Gln Gly Gly Ala Leu Gln Lys Glu Leu
115 120 125
Ile Arg Phe Leu Asn Ala Gly Ile Phe Gly Asn Gly Thr Glu Xaa His
130 135 140
Thr Leu Pro His Ser Ala Thr Arg Ala Ala Met Leu Val Arg Ile Asn
145 150 155 160
Thr Leu Leu Gln Gly Tyr Ser Gly Ile Arg Phe Glu Ile Leu Glu Ala
165 170 175
Ile Thr Lys Leu Leu Asn Asn Asn Ile Thr Pro Cys Leu Pro Leu Arg
180 185 190
Gly Thr Ile Thr Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr Ile Ala
195 200 205
Gly Leu Leu Thr Gly Arg Pro Asn Ser Lys Ala Val Gly Pro Asp Gly
210 215 220
Glu Lys Leu Asn Ala Ala Glu Ala Phe Lys Leu Ala Gly Ile Glu Ser
225 230 235 240
Gly Phe Phe Glu Leu Gln Pro Lys Glu Gly Leu Ala Leu Val Asn Gly
245 250 255
Thr Ala Val Gly Ser Gly Leu Ala Ser Met Val Leu Phe Glu Ala Asn
260 265 270
Val Leu Ala Val Leu Ser Glu Val Leu Ser Ala Ile Phe Ala Glu Val
275 280 285
Met Asn Gly Lys Pro Glu Phe Thr Asp His Leu Thr His Lys Leu Lys
290 295 300
His His Pro Gly Gln Ile Glu Ala Ala Ala Ile Met Glu His Ile Leu
305 310 315 320
Asp Gly Ser Ser Tyr Met Lys Ala Ala Lys Lys Leu His Glu Met Asp
325 330 335
Pro Leu Gln Lys Pro Lys Gln Asp Arg Tyr Ala Leu Arg Thr Ser Pro
340 345 350
Gln Trp Leu Gly Pro Gln Ile Glu Val Ile Arg Ala Ala Thr Lys Ser
355 360 365
Ile Glu Arg Glu Ile Asn Ser Val Asn Asp Asn Pro Leu Ile Asp Val
370 375 380
Ser Arg Asn Lys Ala Leu His Gly Gly Asn Phe Gln Gly Thr Pro Ile
385 390 395 400
Gly Val Ser Met Asp Asn Thr Arg Leu Ala Ile Ala Ala Ile Gly Lys
405 410 415
Leu Met Phe Ala Gln Phe Ser Glu Leu Val Asn Asp Phe Tyr Asn Asn
420 425 430
Gly Leu Pro Ser Asn Leu Ser Xaa Gly Arg Asn Pro Ser Leu Asp Tyr
435 440 445
Gly Phe Lys Gly Ala Glu Ile Ala Met Ala Ser Tyr Cys Ser Glu Leu
450 455 460
Gln Tyr Leu Ala Asn Pro Val Thr Asn His Val Gln Ser Ala Glu Gln
465 470 475 480
His Asn Gln Asp Val Asn Ser Leu Gly Leu Ile Ser Ser Arg Lys Thr
485 490 495
Ala Glu Ala Ile Asp Ile Leu Lys Leu Met Ser Ser Thr Phe Leu Val
500 505 510
Ala Leu Cys Gln Ala Ile Asp Leu Arg His Leu Glu Glu Asn Leu Lys
515 520 525
Ser Ala Val Lys Asn Thr Val Ser Gln Val Ala Lys Lys Thr Leu Thr
530 535 540
Thr Gly Ala Asn Gly Glu Leu His Pro Ser Arg Phe Cys Glu Lys Asp
545 550 555 560
Leu Leu Lys Val Val Asp Arg Glu Tyr Val Phe Ala Tyr Ala Asp Asp
565 570 575
Pro Cys Ser Ala Thr Tyr Pro Leu Met Gln Lys Leu Arg Gln Val Leu
580 585 590
Val Xaa His Ala Leu Ala Asn Gly Glu Asn Glu Lys Asn Ala Asn Thr
595 600 605
Ser Ile Phe Gln Lys Ile Ala Ala Phe Glu Glu Glu Leu Lys Ala Xaa
610 615 620
Leu Pro Lys Glu Val Glu Xaa Ala Arg Ala Ala Tyr Glu Ser Gly Xaa
625 630 635 640
Ala Ala Ile Pro Asn Arg Ile Lys Glu Cys Arg Ser Tyr Pro Leu Tyr
645 650 655
Arg Phe Val Arg Glu Glu Leu Gly Thr Glu Leu Leu Thr Gly Glu Lys
660 665 670
Val Arg Ser Pro Gly Glu Glu Phe Asp Lys Val Phe Thr Ala Met Cys
675 680 685
Gln Gly Lys Ile Ile Asp Pro Leu Leu Glu Cys Leu Lys Glu Trp Asn
690 695 700
Gly Ala Pro Leu Pro Ile Cys
705 710
<210> 97
<211> 714
<212> PRT
<213> 小立碗藓()
<220>
<223> 藓苯丙氨酸氨裂合酶 (PpPAL3)
<400> 97
Met Glu Thr Ile Thr Lys Asn Gly Tyr Gln Asn Gly Ser Ser Glu Ser
1 5 10 15
Leu Cys Thr Gln Arg Asp Pro Leu Ser Trp Gly Val Ala Ala Glu Ala
20 25 30
Met Lys Gly Ser His Leu Asp Glu Val Lys Arg Met Val Ala Glu Tyr
35 40 45
Arg Lys Pro Val Val Asn Leu Ala Gly Gln Thr Leu Thr Ile Ala Gln
50 55 60
Val Ala Ser Ile Ala Gly His Asp Ala Ser Asn Val Lys Val Glu Leu
65 70 75 80
Ser Glu Ser Ala Arg Pro Arg Val Lys Ala Ser Ser Asp Trp Val Met
85 90 95
Asp Ser Met Asp Lys Gly Thr Asp Ser Tyr Gly Val Thr Thr Gly Phe
100 105 110
Gly Ala Thr Ser His Arg Arg Thr Lys Gln Gly Gly Ala Leu Gln Lys
115 120 125
Glu Leu Ile Arg Phe Leu Asn Ala Gly Ile Phe Gly Asn Gly Thr Glu
130 135 140
Thr Cys His Thr Leu Pro His Ser Ala Thr Arg Ala Ala Met Leu Val
145 150 155 160
Arg Ile Asn Thr Leu Leu Gln Gly Tyr Ser Gly Ile Arg Phe Glu Ile
165 170 175
Leu Glu Ala Ile Thr Lys Leu Leu Asn Asn Asn Ile Thr Pro Cys Leu
180 185 190
Pro Leu Arg Gly Thr Ile Thr Ala Ser Gly Asp Leu Val Pro Leu Ser
195 200 205
Tyr Ile Ala Gly Leu Leu Thr Gly Arg Pro Asn Ser Lys Ala Thr Gly
210 215 220
Pro Asn Gly Glu Val Leu Asp Ala Val Glu Ala Phe Lys Ala Ala Gly
225 230 235 240
Ile Asp Ser Gly Phe Phe Glu Leu Gln Pro Lys Glu Gly Leu Ala Leu
245 250 255
Val Asn Gly Thr Ala Val Gly Ser Gly Leu Ala Ser Met Val Leu Phe
260 265 270
Glu Thr Asn Val Leu Ala Val Leu Ser Glu Leu Ile Ser Ala Ile Phe
275 280 285
Ala Glu Val Met Asn Gly Lys Pro Glu Phe Thr Asp His Leu Thr His
290 295 300
Lys Leu Lys His His Pro Gly Gln Ile Glu Ala Ala Ala Ile Met Glu
305 310 315 320
His Ile Leu Asp Gly Ser Ala Tyr Met Lys Ala Ala Lys Lys Leu His
325 330 335
Glu Met Asp Pro Leu Gln Lys Pro Lys Gln Asp Arg Tyr Ala Leu Arg
340 345 350
Thr Ser Pro Gln Trp Leu Gly Pro Gln Ile Glu Val Ile Arg Phe Ser
355 360 365
Thr Lys Ser Ile Glu Arg Glu Ile Asn Ser Val Asn Asp Asn Pro Leu
370 375 380
Ile Asp Val Ser Arg Asn Lys Ala Leu His Gly Gly Asn Phe Gln Gly
385 390 395 400
Thr Pro Ile Gly Val Ser Met Asp Asn Val Arg Leu Ala Ile Ala Ser
405 410 415
Ile Gly Lys Leu Leu Phe Ala Gln Phe Ser Glu Leu Val Asn Asp Phe
420 425 430
Tyr Asn Asn Gly Leu Pro Ser Asn Leu Thr Ala Ser Arg Asn Pro Ser
435 440 445
Leu Asp Tyr Gly Phe Lys Gly Ala Glu Ile Ala Met Ala Ser Tyr Cys
450 455 460
Ser Glu Leu Gln Tyr Leu Ala Asn Pro Val Thr Ser His Val Gln Ser
465 470 475 480
Ala Glu Gln His Asn Gln Asp Val Asn Ser Leu Gly Leu Ile Ser Ser
485 490 495
Arg Lys Thr Ala Glu Ser Val Asp Ile Leu Lys Leu Met Ser Thr Thr
500 505 510
Phe Leu Val Ala Leu Cys Gln Ala Ile Asp Leu Arg His Leu Glu Glu
515 520 525
Asn Leu Arg Ser Ala Val Lys Asn Thr Val Ser His Val Ser Lys Arg
530 535 540
Val Leu Thr Thr Gly Ala Asn Gly Glu Leu His Pro Ser Arg Phe Cys
545 550 555 560
Glu Lys Glu Leu Leu Lys Val Val Asp Arg Glu Asp Val Phe Ala Tyr
565 570 575
Ala Asp Asp Pro Cys Ser Ala Thr Tyr Pro Leu Met Gln Lys Leu Arg
580 585 590
Gln Val Leu Val Asp His Ala Leu Ala Asn Gly Glu Asn Glu Lys Asn
595 600 605
Thr Ser Thr Ser Val Phe Gln Lys Ile Thr Ala Phe Glu Glu Glu Leu
610 615 620
Lys Ala Leu Leu Pro Lys Glu Val Glu Ser Ala Arg Ala Ala Tyr Asp
625 630 635 640
Ser Gly Asn Ser Ala Ile Glu Asn Lys Ile Lys Glu Cys Arg Ser Tyr
645 650 655
Pro Leu Tyr Lys Phe Val Arg Glu Glu Leu Gly Thr Gly Leu Leu Thr
660 665 670
Gly Glu Lys Val Arg Ser Pro Gly Glu Glu Phe Asp Lys Val Phe Thr
675 680 685
Ala Met Cys Gln Gly Lys Ile Ile Asp Pro Met Leu Glu Cys Leu Gly
690 695 700
Glu Trp Asn Gly Ala Pro Leu Pro Ile Cys
705 710
<210> 98
<211> 714
<212> PRT
<213> 水稻()
<220>
<223> 稻苯丙氨酸氨裂合酶 (OsPAL)
<400> 98
Met Glu Cys Glu Asn Gly His Val Ala Ala Ala Ala Asn Gly Ser Ser
1 5 10 15
Leu Cys Val Ala Lys Pro Arg Ala Asp Pro Leu Asn Trp Gly Lys Ala
20 25 30
Ala Glu Glu Leu Ser Gly Ser His Leu Asp Ala Val Lys Arg Met Val
35 40 45
Glu Glu Tyr Arg Arg Pro Val Val Thr Ile Glu Gly Ala Ser Leu Thr
50 55 60
Ile Ala Gln Val Ala Ala Val Ala Ser Ala Gly Ala Ala Arg Val Glu
65 70 75 80
Leu Asp Glu Ser Ala Arg Gly Arg Val Lys Ala Ser Ser Asp Trp Val
85 90 95
Met Asn Ser Met Met Asn Gly Thr Asp Ser Tyr Gly Val Thr Thr Gly
100 105 110
Phe Gly Ala Thr Ser His Arg Arg Thr Lys Glu Gly Gly Ala Leu Gln
115 120 125
Arg Glu Leu Ile Arg Phe Leu Asn Ala Gly Ala Phe Gly Asn Gly Asp
130 135 140
Asp Gly His Val Leu Pro Ala Ala Ala Thr Arg Ala Ala Met Leu Val
145 150 155 160
Arg Ile Asn Thr Leu Leu Gln Gly Tyr Ser Gly Ile Arg Phe Glu Ile
165 170 175
Leu Glu Thr Ile Ala Thr Leu Leu Asn Ala Asn Val Thr Pro Cys Leu
180 185 190
Pro Leu Arg Gly Thr Ile Thr Ala Ser Gly Asp Leu Val Pro Leu Ser
195 200 205
Tyr Ile Ala Gly Leu Val Thr Gly Arg Pro Asn Ser Val Ala Val Thr
210 215 220
Pro Asp Gly Arg Lys Val Asp Ala Ala Glu Ala Phe Lys Ile Ala Gly
225 230 235 240
Ile Gln His Gly Phe Phe Glu Leu Gln Pro Lys Glu Gly Leu Ala Met
245 250 255
Val Asn Gly Thr Ala Val Gly Ser Gly Leu Ala Ser Met Val Leu Phe
260 265 270
Glu Ala Asn Val Leu Gly Val Leu Ala Glu Val Leu Ser Ala Val Phe
275 280 285
Cys Glu Val Met Asn Gly Lys Pro Glu Tyr Thr Asp His Leu Thr His
290 295 300
Lys Leu Lys His His Pro Gly Gln Ile Glu Ala Ala Ala Ile Met Glu
305 310 315 320
His Ile Leu Glu Gly Ser Ser Tyr Met Met Leu Ala Lys Lys Leu Gly
325 330 335
Glu Leu Asp Pro Leu Met Lys Pro Lys Gln Asp Arg Tyr Ala Leu Arg
340 345 350
Thr Ser Pro Gln Trp Leu Gly Pro Gln Ile Glu Val Ile Arg Ala Ala
355 360 365
Thr Lys Ser Ile Glu Arg Glu Ile Asn Ser Val Asn Asp Asn Pro Leu
370 375 380
Ile Asp Val Ser Arg Gly Lys Ala Leu His Gly Gly Asn Phe Gln Gly
385 390 395 400
Thr Pro Ile Gly Val Ser Met Asp Asn Thr Arg Leu Ala Ile Ala Ala
405 410 415
Ile Gly Lys Leu Met Phe Ala Gln Phe Ser Glu Leu Val Asn Asp Phe
420 425 430
Tyr Asn Asn Gly Leu Pro Ser Asn Leu Ser Gly Gly Arg Asn Pro Ser
435 440 445
Leu Asp Tyr Gly Phe Lys Gly Ala Glu Ile Ala Met Ala Ser Tyr Cys
450 455 460
Ser Glu Leu Gln Phe Leu Ala Asn Pro Val Thr Asn His Val Gln Ser
465 470 475 480
Ala Glu Gln His Asn Gln Asp Val Asn Ser Leu Gly Leu Ile Ser Ser
485 490 495
Arg Lys Thr Ala Glu Ala Ile Asp Val Leu Lys Leu Met Ser Ser Thr
500 505 510
Phe Leu Ile Ala Leu Cys Gln Ala Ile Asp Leu Arg His Leu Glu Glu
515 520 525
Asn Val Arg Ser Ala Val Lys Gly Cys Val Thr Thr Val Ala Arg Lys
530 535 540
Thr Leu Ser Thr Ser Ala Thr Gly Asp Leu His Lys Ala Arg Phe Cys
545 550 555 560
Glu Lys Asp Leu Leu Gln Ala Ile Asp Arg Glu Ala Val Phe Ala Tyr
565 570 575
Ala Asp Asp Pro Cys Ser Ala Asn Tyr Pro Leu Met Gln Lys Met Arg
580 585 590
Ala Val Leu Ile Glu His Ala Leu Ala Asn Gly Glu Ala Glu Arg Asn
595 600 605
Val Asp Thr Ser Val Phe Ala Lys Val Ala Thr Phe Glu Glu Glu Leu
610 615 620
Arg Val Ala Leu Pro Arg Glu Val Glu Ala Ala Arg Ala Ala Val Glu
625 630 635 640
Asn Gly Thr Ala Ala Lys Ala Asn Arg Ile Thr Glu Cys Arg Ser Tyr
645 650 655
Pro Leu Tyr Arg Phe Val Arg Glu Glu Leu Gly Thr Glu Tyr Leu Thr
660 665 670
Gly Glu Lys Thr Arg Ser Pro Gly Glu Glu Val Asn Lys Val Phe Val
675 680 685
Ala Met Asn Gln Gly Lys His Ile Asp Ala Leu Leu Glu Cys Leu Lys
690 695 700
Glu Trp Asn Gly Glu Pro Leu Pro Ile Cys
705 710
<210> 99
<211> 715
<212> PRT
<213> 玉米()
<220>
<223> 玉米苯丙氨酸氨裂合酶 (ZmPAL)
<400> 99
Met Glu Cys Glu Asn Gly His Val Ala Ala Ser Gly Asn Gly Gly Val
1 5 10 15
Cys Leu Ala Thr Pro Ala Pro Arg Ala Asp Pro Leu Asn Trp Ala Lys
20 25 30
Ala Ala Glu Asp Leu Ala Gly Ser His Leu Asp Ala Val Lys Arg Met
35 40 45
Val Glu Glu Tyr Arg Arg Pro Leu Val Lys Ile Glu Gly Ala Ser Leu
50 55 60
Thr Val Ala Gln Val Ala Ala Val Ala Ala Ala Gly Glu Ala Arg Val
65 70 75 80
Glu Leu Asp Glu Ser Ala Arg Gly Arg Val Lys Ala Ser Ser Asp Trp
85 90 95
Val Met Asp Ser Met Met Asn Gly Thr Asp Ser Tyr Gly Val Thr Thr
100 105 110
Gly Phe Gly Ala Thr Ser His Arg Arg Thr Lys Glu Gly Gly Ala Leu
115 120 125
Gln Arg Glu Leu Ile Arg Phe Leu Asn Ala Gly Ala Phe Gly Thr Gly
130 135 140
Asp Asp Gly His Val Leu Pro Ala Ala Ala Thr Arg Ala Ala Met Leu
145 150 155 160
Val Arg Ile Asn Thr Leu Leu Gln Gly Tyr Ser Gly Ile Arg Phe Glu
165 170 175
Ile Leu Glu Ala Ile Ala Val Leu Leu Asn Ala Asn Val Thr Pro Cys
180 185 190
Leu Pro Leu Arg Gly Thr Ile Thr Ala Ser Gly Asp Leu Val Pro Leu
195 200 205
Ser Tyr Ile Ala Gly Leu Val Thr Gly Arg Pro Asn Ser Thr Ala Val
210 215 220
Ala Pro Asp Gly Arg Lys Val Gly Ala Ala Glu Ala Phe Glu Ile Ala
225 230 235 240
Gly Ile Gln His Gly Phe Phe Glu Leu Gln Pro Lys Glu Gly Leu Ala
245 250 255
Met Val Asn Gly Thr Ala Val Gly Ser Gly Leu Ala Ser Met Val Leu
260 265 270
Phe Glu Ala Asn Val Leu Ala Val Leu Ala Glu Val Met Ser Ala Val
275 280 285
Phe Cys Glu Val Met Asn Gly Lys Pro Glu Tyr Thr Asp His Leu Thr
290 295 300
His Lys Leu Lys His His Pro Gly Gln Ile Glu Ser Ala Ala Ile Met
305 310 315 320
Glu His Ile Leu Glu Gly Ser Ser Tyr Met Ala Leu Ala Lys Lys Leu
325 330 335
Gly Glu Leu Asp Pro Leu Met Lys Pro Lys Gln Asp Arg Tyr Ala Leu
340 345 350
Arg Thr Ser Pro Gln Trp Leu Gly Pro Gln Ile Glu Val Ile Arg Ala
355 360 365
Ala Thr Lys Ser Ile Glu Arg Glu Ile Asn Ser Val Asn Asp Asn Pro
370 375 380
Leu Ile Asp Val Ser Arg Gly Lys Ala Leu His Gly Gly Asn Phe Gln
385 390 395 400
Gly Thr Pro Ile Gly Val Ser Met Asp Asn Thr Arg Leu Ala Val Ala
405 410 415
Ala Ile Gly Lys Leu Met Phe Ala Gln Phe Ser Glu Leu Val Asn Asp
420 425 430
Phe Tyr Asn Asn Gly Leu Pro Ser Asn Leu Ser Gly Gly Arg Asn Pro
435 440 445
Ser Leu Asp Tyr Gly Phe Lys Gly Ala Glu Ile Ala Met Ala Ser Tyr
450 455 460
Cys Ser Glu Leu Gln Phe Leu Ala Asn Pro Val Thr Asn His Val Gln
465 470 475 480
Ser Ala Glu Gln His Asn Gln Asp Val Asn Ser Leu Gly Leu Ile Ser
485 490 495
Ser Arg Lys Thr Ala Glu Ala Val Asp Val Leu Lys Leu Met Ser Ser
500 505 510
Thr Phe Leu Val Ala Leu Cys Gln Ala Ile Asp Leu Arg His Leu Glu
515 520 525
Glu Asn Leu Arg Ser Ala Val Lys Arg Cys Val Thr Ala Val Ala Arg
530 535 540
Lys Thr Leu Ser Thr Gly Ala Thr Gly Ala Leu His Asp Ala Arg Phe
545 550 555 560
Cys Glu Lys Asp Leu Leu Thr Ala Val Asp Arg Glu Ala Val Phe Ala
565 570 575
Tyr Ala Asp Asp Pro Cys Ser Ala Thr Tyr Pro Leu Met Gln Lys Met
580 585 590
Arg Ser Val Leu Val Glu His Ala Leu Ala Asn Gly Glu Ala Glu Arg
595 600 605
Asp Pro Asp Thr Ser Val Phe Ala Lys Val Ala Thr Phe Glu Glu Glu
610 615 620
Leu Arg Ala Ala Leu Pro Arg Glu Val Asp Ala Ala Arg Ala Ala Val
625 630 635 640
Glu Ser Gly Thr Ala Ala Ile Arg Asn Arg Ile Ala Glu Cys Arg Ser
645 650 655
Tyr Pro Leu Tyr Arg Phe Val Arg Glu Glu Leu Gly Thr Glu Tyr Leu
660 665 670
Thr Gly Glu Lys Ala Arg Ser Pro Gly Glu Glu Val Asp Lys Val Phe
675 680 685
Val Ala Met Asn Leu Gly Lys His Ile Asp Ala Val Leu Glu Cys Leu
690 695 700
Lys Glu Trp Asn Gly Glu Pro Leu Pro Ile Cys
705 710 715
<210> 100
<211> 704
<212> PRT
<213> 两色高梁()
<220>
<223> 高粱苯丙氨酸氨裂合酶 (SbPAL)
<400> 100
Met Ala Gly Asn Gly Ala Ile Val Glu Ser Asp Pro Leu Asn Trp Gly
1 5 10 15
Ala Ala Ala Ala Glu Leu Ser Gly Ser His Leu Asp Glu Val Lys Arg
20 25 30
Met Val Ala Gln Ala Arg Gln Pro Val Val Lys Ile Glu Gly Ser Thr
35 40 45
Leu Arg Val Gly Gln Val Ala Ala Val Ala Ser Ala Lys Asp Ala Ser
50 55 60
Gly Val Ala Val Glu Leu Asp Glu Glu Ala Arg Pro Arg Val Lys Ala
65 70 75 80
Ser Ser Glu Trp Ile Leu Asp Cys Ile Ala His Gly Gly Asp Ile Tyr
85 90 95
Gly Val Thr Thr Gly Phe Gly Gly Thr Ser His Arg Arg Thr Lys Asp
100 105 110
Gly Pro Ala Leu Gln Val Glu Leu Leu Arg His Leu Asn Ala Gly Ile
115 120 125
Phe Gly Thr Gly Ser Asp Gly His Thr Leu Pro Ser Glu Val Val Arg
130 135 140
Ala Ala Met Leu Val Arg Ile Asn Thr Leu Leu Gln Gly Tyr Ser Gly
145 150 155 160
Ile Arg Phe Glu Ile Leu Glu Ala Ile Thr Lys Leu Leu Asn Thr Gly
165 170 175
Val Ser Pro Cys Leu Pro Leu Arg Gly Thr Ile Thr Ala Ser Gly Asp
180 185 190
Leu Val Pro Leu Ser Tyr Ile Ala Gly Leu Ile Thr Gly Arg Pro Asn
195 200 205
Ala Gln Ala Thr Thr Val Asp Gly Arg Lys Val Asp Ala Ala Glu Ala
210 215 220
Phe Lys Ile Ala Gly Ile Glu Gly Gly Phe Phe Lys Leu Asn Pro Lys
225 230 235 240
Glu Gly Leu Ala Ile Val Asn Gly Thr Ser Val Gly Ser Ala Leu Ala
245 250 255
Ala Thr Val Met Tyr Asp Ala Asn Val Leu Ala Val Leu Ser Glu Val
260 265 270
Leu Ser Ala Ile Phe Cys Glu Val Met Asn Gly Lys Pro Glu Tyr Thr
275 280 285
Asp His Leu Thr His Lys Leu Lys His His Pro Gly Ser Ile Glu Ala
290 295 300
Ala Ala Ile Met Glu His Ile Leu Asp Gly Ser Ala Phe Met Lys His
305 310 315 320
Ala Lys Lys Val Asn Glu Leu Asp Pro Leu Leu Lys Pro Lys Gln Asp
325 330 335
Arg Tyr Ala Leu Arg Thr Ser Pro Gln Trp Leu Gly Pro Gln Ile Glu
340 345 350
Val Leu Arg Ala Ala Thr Lys Ser Ile Glu Arg Glu Val Asn Ser Val
355 360 365
Asn Asp Asn Pro Val Ile Asp Val His Arg Gly Lys Ala Leu His Gly
370 375 380
Gly Asn Phe Gln Gly Thr Pro Ile Gly Val Ser Met Asp Asn Ala Arg
385 390 395 400
Leu Ala Ile Ala Asn Ile Gly Lys Leu Met Phe Ala Gln Phe Ser Glu
405 410 415
Leu Val Asn Glu Phe Tyr Asn Asn Gly Leu Thr Ser Asn Leu Ala Gly
420 425 430
Ser Arg Asn Pro Ser Leu Asp Tyr Gly Phe Lys Gly Thr Glu Ile Ala
435 440 445
Met Ala Ser Tyr Cys Ser Glu Leu Gln Tyr Leu Gly Asn Pro Ile Thr
450 455 460
Asn His Val Gln Ser Ala Glu Gln His Asn Gln Asp Val Asn Ser Leu
465 470 475 480
Gly Leu Val Ser Ala Arg Lys Thr Ala Glu Ala Ile Asp Ile Leu Lys
485 490 495
Leu Met Ser Ser Thr Tyr Ile Val Ala Leu Cys Gln Ala Ile Asp Leu
500 505 510
Arg His Leu Glu Glu Asn Ile Lys Thr Ser Val Lys Asn Thr Val Thr
515 520 525
Gln Val Ala Lys Lys Val Leu Thr Met Asn Pro Ser Gly Asp Leu Ser
530 535 540
Ser Ala Arg Phe Ser Glu Lys Glu Leu Ile Thr Ala Ile Asp Arg Glu
545 550 555 560
Gly Val Phe Thr Tyr Ala Glu Asp Pro Ala Ser Ala Ser Leu Pro Leu
565 570 575
Met Thr Lys Leu Arg Ala Val Leu Val Asp His Ala Leu Ser Ser Gly
580 585 590
Asp Ala Glu Arg Glu Pro Ser Val Phe Ser Lys Ile Thr Lys Phe Glu
595 600 605
Glu Glu Leu Arg Ala Val Leu Pro Arg Glu Val Glu Ala Ala Arg Val
610 615 620
Ala Val Ala Glu Gly Thr Ala Pro Val Ala Asn Arg Ile Ala Asp Ser
625 630 635 640
Arg Ser Phe Pro Leu Tyr Arg Phe Val Arg Glu Glu Leu Gly Cys Val
645 650 655
Phe Leu Thr Gly Glu Lys Leu Lys Ser Pro Gly Glu Glu Cys Thr Lys
660 665 670
Val Phe Asn Gly Ile Asn Gln Gly Lys Leu Val Asp Pro Met Leu Glu
675 680 685
Cys Leu Lys Glu Trp Asp Gly Lys Pro Leu Pro Ile Asn Val Val Asn
690 695 700
<210> 101
<211> 718
<212> PRT
<213> 马尾松()
<220>
<223> 松树苯丙氨酸氨裂合酶 (PlPAL)
<400> 101
Met Val Ala Ala Ala Glu Met Thr Gln Ala Asn Glu Val Gln Val Lys
1 5 10 15
Ser Thr Gly Leu Cys Thr Asp Phe Gly Ser Ser Gly Ser Asp Pro Leu
20 25 30
Asn Trp Val Arg Ala Ala Lys Ala Met Glu Gly Ser His Phe Glu Glu
35 40 45
Val Lys Ala Met Val Asp Ser Tyr Leu Gly Val Lys Glu Ile Phe Ile
50 55 60
Glu Gly Lys Ser Leu Thr Ile Ser Asp Val Ala Ala Val Ala Arg Arg
65 70 75 80
Ser Gln Val Lys Val Lys Leu Asp Ala Glu Ala Ala Lys Ser Arg Val
85 90 95
Glu Glu Ser Ser Asn Trp Val Leu Thr Gln Met Asn Lys Gly Thr Asp
100 105 110
Thr Tyr Gly Val Thr Thr Gly Phe Gly Ala Thr Ser His Arg Arg Thr
115 120 125
Asn Gln Gly Ala Glu Leu Gln Lys Glu Leu Ile Arg Phe Leu Asn Ala
130 135 140
Gly Val Leu Gly Lys Cys Pro Glu Asn Val Leu Ser Glu Asp Thr Thr
145 150 155 160
Arg Ala Ala Met Leu Val Arg Thr Asn Thr Leu Leu Gln Gly Tyr Ser
165 170 175
Gly Ile Arg Trp Asp Ile Leu Glu Thr Val Glu Lys Leu Leu Asn Ala
180 185 190
Gly Leu Thr Pro Lys Leu Pro Leu Arg Gly Thr Ile Thr Ala Ser Gly
195 200 205
Asp Leu Val Pro Leu Ser Tyr Ile Ala Gly Leu Leu Thr Gly Arg Pro
210 215 220
Asn Ser Arg Val Arg Ser Arg Asp Gly Ile Glu Met Ser Gly Ala Glu
225 230 235 240
Ala Leu Lys Lys Val Gly Leu Glu Lys Pro Phe Glu Leu Gln Pro Lys
245 250 255
Glu Gly Leu Ala Ile Val Asn Gly Thr Ser Val Gly Ala Ala Leu Ala
260 265 270
Ser Ile Val Cys Phe Asp Ala Asn Val Leu Ala Leu Leu Ser Glu Val
275 280 285
Ile Ser Ala Met Phe Cys Glu Val Met Asn Gly Lys Pro Glu Phe Thr
290 295 300
Asp Pro Leu Thr His Lys Leu Lys His His Pro Gly Gln Met Glu Ala
305 310 315 320
Ala Ala Ile Met Glu Tyr Val Leu Asp Gly Ser Ser Tyr Met Lys His
325 330 335
Ala Ala Lys Leu His Glu Met Asn Pro Leu Gln Lys Pro Lys Gln Asp
340 345 350
Arg Tyr Ala Leu Arg Thr Ser Pro Gln Trp Leu Gly Pro Gln Val Glu
355 360 365
Ile Ile Arg Ser Ala Thr His Met Ile Glu Arg Glu Ile Asn Ser Val
370 375 380
Asn Asp Asn Pro Val Ile Asp Val Ala Arg Asp Lys Ala Leu His Gly
385 390 395 400
Gly Asn Phe Gln Gly Thr Pro Ile Gly Val Ser Met Asp Asn Leu Arg
405 410 415
Leu Ser Ile Ser Ala Ile Gly Lys Leu Met Phe Ala Gln Phe Ser Glu
420 425 430
Leu Val Asn Asp Tyr Tyr Asn Gly Gly Leu Pro Ser Asn Leu Ser Gly
435 440 445
Gly Pro Asn Pro Ser Leu Asp Tyr Gly Leu Lys Gly Ala Glu Ile Ala
450 455 460
Met Ala Ser Tyr Thr Ser Glu Leu Leu Tyr Leu Ala Asn Pro Val Thr
465 470 475 480
Ser His Val Gln Ser Ala Glu Gln His Asn Gln Asp Val Asn Ser Leu
485 490 495
Gly Leu Val Ser Ala Arg Lys Ser Ala Glu Ala Ile Asp Ile Leu Lys
500 505 510
Leu Met Leu Ser Thr Tyr Leu Thr Ala Leu Cys Gln Ala Val Asp Leu
515 520 525
Arg His Leu Glu Glu Asn Met Leu Ala Thr Val Lys Gln Ile Val Ser
530 535 540
Gln Val Ala Lys Lys Thr Leu Ser Thr Gly Leu Asn Gly Glu Leu Leu
545 550 555 560
Pro Gly Arg Phe Cys Glu Lys Asp Leu Leu Gln Val Val Asp Asn Glu
565 570 575
His Val Phe Ser Tyr Ile Asp Asp Pro Cys Asn Ala Ser Tyr Pro Leu
580 585 590
Thr Gln Lys Leu Arg Asn Ile Leu Val Glu His Ala Phe Lys Asn Ala
595 600 605
Glu Gly Glu Lys Asp Pro Asn Thr Ser Ile Phe Asn Lys Ile Pro Leu
610 615 620
Phe Glu Ala Glu Leu Lys Ala Gln Leu Glu Leu Gln Val Ser Leu Ala
625 630 635 640
Arg Glu Ser Tyr Asp Lys Gly Thr Ser Pro Leu Pro Asn Arg Ile Gln
645 650 655
Glu Cys Arg Ser Tyr Pro Leu Tyr Glu Phe Val Arg Asn Gln Leu Gly
660 665 670
Thr Lys Leu Leu Ser Gly Thr Arg Thr Thr Ser Pro Gly Glu Val Ile
675 680 685
Glu Val Val Tyr Asp Ala Ile Ser Glu Asp Lys Val Ile Val Pro Leu
690 695 700
Phe Lys Cys Leu Asp Gly Trp Lys Gly Thr Pro Gly Pro Phe
705 710 715
<210> 102
<211> 725
<212> PRT
<213> 紫苜蓿()
<220>
<223> 苜蓿苯丙氨酸氨裂合酶 (MsPAL)
<400> 102
Met Glu Thr Ile Ser Ala Ala Ile Thr Lys Asn Asn Ala Asn Glu Ser
1 5 10 15
Phe Cys Leu Ile His Ala Lys Asn Asn Asn Asn Met Lys Val Asn Glu
20 25 30
Ala Asp Pro Leu Asn Trp Gly Val Ala Ala Glu Ala Met Lys Gly Ser
35 40 45
His Leu Asp Glu Val Lys Arg Met Val Ala Glu Tyr Arg Lys Pro Val
50 55 60
Val Arg Leu Gly Gly Glu Thr Leu Thr Ile Ser Gln Val Ala Ala Ile
65 70 75 80
Ala Ala His Asp His Gly Val Gln Val Asp Leu Ser Glu Ser Ala Arg
85 90 95
Asp Gly Val Lys Ala Ser Ser Glu Trp Val Met Glu Ser Met Asn Lys
100 105 110
Gly Thr Asp Ser Tyr Gly Val Thr Thr Gly Phe Gly Ala Thr Ser His
115 120 125
Ser Arg Thr Lys Gln Gly Gly Ala Leu Gln Lys Glu Leu Ile Arg Phe
130 135 140
Leu Asn Ala Gly Ile Phe Gly Asn Gly Thr Glu Ser Asn His Thr Leu
145 150 155 160
Pro Lys Thr Ala Thr Arg Ala Ala Met Leu Val Arg Ile Asn Thr Leu
165 170 175
Leu Gln Gly Tyr Ser Gly Ile Asp Phe Glu Ile Leu Glu Ala Ile Thr
180 185 190
Lys Pro Leu Asn Lys Thr Val Thr Pro Cys Leu Pro Leu Arg Gly Thr
195 200 205
Ile Thr Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr Ile Ala Gly Leu
210 215 220
Leu Thr Gly Arg Pro Asn Ser Lys Ala His Gly Pro Ser Gly Glu Val
225 230 235 240
Leu Asn Ala Lys Glu Ala Phe Asn Leu Ala Gly Ile Asn Ala Glu Phe
245 250 255
Phe Glu Leu Gln Pro Lys Glu Gly Leu Ala Leu Val Asn Gly Thr Ala
260 265 270
Val Gly Ser Gly Leu Ala Ser Ile Val Leu Phe Glu Ala Asn Ile Leu
275 280 285
Ala Val Leu Ser Glu Val Leu Ser Ala Ile Phe Ala Glu Val Met Gln
290 295 300
Gly Lys Pro Glu Phe Thr Asp His Leu Thr His Lys Leu Lys His His
305 310 315 320
Pro Gly Gln Ile Glu Ala Ala Ala Ile Met Glu His Ile Leu Asp Gly
325 330 335
Ser Ser Tyr Val Lys Ala Ala Lys Lys Leu His Glu Ile Asp Pro Leu
340 345 350
Gln Lys Pro Lys Gln Asp Arg Tyr Ala Leu Arg Thr Ser Pro Gln Trp
355 360 365
Leu Gly Pro Leu Val Glu Val Ile Arg Phe Ser Thr Lys Ser Ile Glu
370 375 380
Arg Glu Ile Asn Ser Val Asn Asp Asn Pro Leu Ile Asp Val Ser Arg
385 390 395 400
Asn Lys Ala Leu His Gly Gly Asn Phe Gln Gly Thr Pro Ile Gly Val
405 410 415
Ser Met Asp Asn Thr Arg Leu Ala Leu Ala Ser Ile Gly Lys Leu Met
420 425 430
Phe Ala Gln Phe Ser Glu Leu Val Asn Asp Phe Tyr Asn Asn Gly Leu
435 440 445
Pro Ser Asn Leu Ser Ala Ser Arg Asn Pro Ser Leu Asp Tyr Gly Phe
450 455 460
Lys Gly Ala Glu Ile Ala Met Ala Ser Tyr Cys Ser Glu Leu Gln Tyr
465 470 475 480
Leu Ala Asn Pro Val Thr Thr His Val Gln Ser Ala Glu Gln His Asn
485 490 495
Gln Asp Val Asn Ser Leu Gly Leu Ile Ser Ala Arg Lys Thr Asn Glu
500 505 510
Ala Ile Glu Ile Leu Gln Leu Met Ser Ser Thr Phe Leu Ile Ala Leu
515 520 525
Cys Gln Ala Ile Asp Leu Arg His Leu Glu Glu Asn Leu Lys Asn Ser
530 535 540
Val Lys Asn Thr Val Ser Gln Val Ala Lys Lys Thr Leu Thr Met Gly
545 550 555 560
Val Asn Gly Glu Leu His Pro Ser Arg Phe Cys Glu Lys Asp Leu Leu
565 570 575
Lys Val Val Asp Arg Glu His Val Phe Ala Tyr Ile Asp Asp Pro Cys
580 585 590
Ser Ala Thr Tyr Pro Leu Ser Gln Lys Leu Arg Gln Val Leu Val Asp
595 600 605
His Ala Leu Val Asn Gly Glu Ser Glu Lys Asn Phe Asn Thr Ser Ile
610 615 620
Phe Gln Lys Ile Ala Thr Phe Glu Glu Glu Leu Lys Thr Leu Leu Pro
625 630 635 640
Lys Glu Val Glu Ser Ala Arg Thr Ala Tyr Glu Ser Gly Asn Pro Thr
645 650 655
Ile Pro Asn Lys Ile Asn Gly Cys Arg Ser Tyr Pro Leu Tyr Lys Phe
660 665 670
Val Arg Glu Glu Leu Gly Thr Gly Leu Leu Thr Gly Glu Asn Val Ile
675 680 685
Ser Pro Gly Glu Glu Cys Asp Lys Leu Phe Ser Ala Met Cys Gln Gly
690 695 700
Lys Ile Ile Asp Pro Leu Leu Glu Cys Leu Gly Glu Trp Asn Gly Ala
705 710 715 720
Pro Leu Pro Ile Cys
725
<210> 103
<211> 700
<212> PRT
<213> 小麦()
<220>
<223> 小麦苯丙氨酸氨裂合酶 (TaPAL)
<400> 103
Met Ala Cys Ala Trp Arg Ser Arg Ser Arg Ala Asp Pro Leu Asn Trp
1 5 10 15
Gly Lys Ala Ala Glu Glu Leu Ser Gly Ser His Leu Glu Ala Val Lys
20 25 30
Arg Met Val Glu Glu Tyr Arg Lys Pro Val Val Thr Met Glu Gly Ala
35 40 45
Thr Thr Ile Ala Met Val Ala Ala Val Ala Ala Gly Ser Asp Thr Arg
50 55 60
Val Glu Leu Asp Glu Ser Ala Arg Gly Arg Val Lys Glu Ser Ser Asp
65 70 75 80
Trp Val Met Asn Ser Met Met Asn Gly Thr Asp Ser Tyr Gly Val Thr
85 90 95
Thr Gly Phe Gly Ala Thr Ser His Arg Arg Thr Lys Glu Gly Gly Ala
100 105 110
Leu Gln Arg Glu Leu Ile Arg Phe Leu Asn Ala Gly Ala Phe Gly Thr
115 120 125
Gly Thr Asp Gly His Val Leu Pro Ala Ala Ala Thr Arg Ala Ala Met
130 135 140
Leu Val Arg Val Asn Thr Leu Leu Gln Gly Tyr Ser Gly Ile Arg Phe
145 150 155 160
Glu Ile Leu Glu Thr Ile Ala Thr Leu Leu Asn Ala Asn Val Thr Pro
165 170 175
Cys Leu Pro Leu Arg Gly Thr Ile Thr Ala Ser Gly Asp Leu Val Pro
180 185 190
Leu Ser Tyr Ile Ala Gly Leu Val Thr Gly Arg Pro Asn Ser Met Ala
195 200 205
Thr Ala Pro Asp Gly Ser Lys Val Asn Ala Ala Glu Ala Phe Lys Ile
210 215 220
Ala Gly Ile Gln His Gly Phe Phe Glu Leu Gln Pro Lys Glu Gly Leu
225 230 235 240
Ala Met Val Asn Gly Thr Ala Val Gly Ser Gly Leu Ala Ser Met Val
245 250 255
Leu Phe Glu Ala Asn Val Leu Ser Leu Leu Ala Glu Val Leu Ser Gly
260 265 270
Val Phe Cys Glu Val Met Asn Gly Lys Pro Glu Phe Thr Asp His Leu
275 280 285
Thr His Lys Leu Lys His His Pro Gly Gln Ile Glu Ala Ala Ala Ile
290 295 300
Met Glu His Ile Leu Glu Gly Ser Ser Tyr Met Met Leu Ala Lys Lys
305 310 315 320
Leu Gly Glu Leu Asp Pro Leu Met Lys Pro Lys Gln Asp Arg Tyr Ala
325 330 335
Leu Arg Thr Ser Pro Gln Trp Leu Gly Pro Gln Ile Glu Val Ile Arg
340 345 350
Ala Ala Thr Lys Ser Ile Glu Arg Glu Ile Asn Ser Val Asn Asp Asn
355 360 365
Pro Leu Ile Asp Val Ser Arg Gly Lys Ala Ile His Gly Gly Asn Phe
370 375 380
Gln Gly Thr Pro Ile Gly Val Ser Met Asp Asn Thr Arg Leu Ala Ile
385 390 395 400
Ala Ala Ile Gly Lys Leu Met Phe Ala Gln Phe Ser Glu Leu Val Asn
405 410 415
Asp Phe Tyr Asn Asn Gly Leu Pro Ser Asn Leu Ser Gly Gly Arg Asn
420 425 430
Pro Ser Leu Asp Tyr Gly Phe Lys Gly Ala Glu Ile Ala Met Ala Ser
435 440 445
Tyr Cys Ser Glu Leu Gln Phe Leu Gly Asn Pro Val Thr Asn His Val
450 455 460
Gln Ser Ala Glu Gln His Asn Gln Asp Val Asn Ser Leu Gly Leu Ile
465 470 475 480
Ser Ser Arg Lys Thr Ala Glu Ala Ile Asp Ile Leu Lys Leu Met Ser
485 490 495
Ser Thr Phe Leu Val Ala Leu Cys Gln Ala Ile Asp Leu Arg His Leu
500 505 510
Glu Glu Asn Val Lys Asn Ala Val Lys Ser Cys Val Lys Thr Val Ala
515 520 525
Arg Lys Thr Leu Ser Thr Asp Asn Asn Gly His Leu His Asn Ala Arg
530 535 540
Phe Cys Glu Lys Asp Leu Leu Leu Thr Ile Asp Arg Glu Ala Val Phe
545 550 555 560
Ala Tyr Ala Asp Asp Pro Cys Ser Ala Asn Tyr Pro Leu Met Gln Lys
565 570 575
Met Arg Ala Val Leu Val Glu His Ala Leu Ala Asn Gly Glu Ala Glu
580 585 590
Ala His Val Glu Thr Ser Val Phe Ala Lys Leu Ala Met Phe Glu Gln
595 600 605
Glu Leu Arg Ala Val Leu Pro Lys Glu Val Glu Ala Ala Arg Ser Ala
610 615 620
Val Glu Asn Gly Thr Ala Ala Gln Gln Asn Arg Ile Ala Glu Cys Arg
625 630 635 640
Ser Tyr Pro Leu Tyr Arg Phe Val Arg Lys Glu Leu Gly Thr Glu Tyr
645 650 655
Leu Thr Gly Glu Lys Thr Arg Ser Pro Gly Glu Glu Val Asp Lys Val
660 665 670
Phe Val Ala Met Asn Gln Gly Lys His Ile Asp Ala Leu Leu Glu Cys
675 680 685
Leu Lys Glu Trp Asn Gly Glu Pro Leu Pro Leu Cys
690 695 700
<210> 104
<211> 717
<212> PRT
<213> 大豆()
<220>
<223> 大豆苯丙氨酸氨裂合酶 (GmPAL2)
<400> 104
Met Ala Ser Glu Ala Asn Ala Ala Asn Thr Asn Phe Cys Val Asn Val
1 5 10 15
Ser Asn Asn Gly Tyr Ile Ser Ala Asn Asp Pro Leu Asn Trp Gly Ala
20 25 30
Ala Ala Glu Ala Met Ala Gly Ser His Leu Asp Glu Val Lys Arg Met
35 40 45
Leu Glu Glu Tyr Arg Arg Pro Val Val Lys Leu Gly Gly Glu Thr Leu
50 55 60
Thr Ile Ser Gln Val Ala Ala Ile Ala Ala His Asp Gln Gly Val Lys
65 70 75 80
Val Glu Leu Ala Glu Ser Ser Arg Ala Gly Val Lys Ala Ser Ser Asp
85 90 95
Trp Val Met Glu Ser Met Asn Lys Gly Thr Asp Ser Tyr Gly Val Thr
100 105 110
Thr Gly Phe Gly Ala Thr Ser His Arg Arg Thr Lys Gln Gly Ala Ala
115 120 125
Leu Gln Lys Glu Leu Ile Arg Phe Leu Asn Ala Gly Ile Phe Gly Asn
130 135 140
Gly Thr Glu Ser Asn Cys Thr Leu Pro His Thr Ala Thr Arg Ala Ala
145 150 155 160
Met Leu Val Arg Ile Asn Thr Leu Leu Gln Gly Tyr Ser Gly Ile Arg
165 170 175
Phe Glu Ile Leu Glu Ala Ile Thr Lys Leu Leu Asn Asn Asn Ile Thr
180 185 190
Pro Cys Leu Pro Leu Arg Gly Thr Ile Thr Ala Ser Gly Asp Leu Val
195 200 205
Pro Leu Ser Tyr Ile Ala Gly Leu Leu Thr Gly Arg Pro Asn Ser Lys
210 215 220
Ala Val Gly Pro Ser Gly Glu Ile Leu Asn Ala Lys Glu Ala Phe Glu
225 230 235 240
Leu Ala Asn Ile Gly Ala Glu Phe Phe Glu Leu Gln Pro Lys Glu Gly
245 250 255
Leu Ala Leu Val Asn Gly Thr Ala Val Gly Ser Gly Leu Ala Ser Ile
260 265 270
Val Leu Phe Glu Ala Asn Ile Ile Ala Val Leu Ser Glu Val Ile Ser
275 280 285
Ala Ile Phe Ala Glu Val Met Gln Gly Lys Pro Glu Phe Thr Asp His
290 295 300
Leu Thr His Lys Leu Lys His His Pro Gly Gln Ile Glu Ala Ala Ala
305 310 315 320
Ile Met Glu His Ile Leu Glu Gly Ser Ser Tyr Val Lys Ala Ala Lys
325 330 335
Lys Leu His Glu Ile Asp Pro Leu Gln Lys Pro Lys Gln Asp Arg Tyr
340 345 350
Ala Leu Arg Thr Ser Pro Gln Trp Leu Gly Pro Leu Ile Glu Val Ile
355 360 365
Arg Phe Ser Thr Lys Ser Ile Glu Arg Glu Ile Asn Ser Val Asn Asp
370 375 380
Asn Pro Leu Ile Asp Val Ser Arg Asn Lys Ala Leu His Gly Gly Asn
385 390 395 400
Phe Gln Gly Thr Pro Ile Gly Val Ser Met Asp Asn Thr Arg Leu Ala
405 410 415
Leu Ala Ser Ile Gly Lys Leu Met Phe Ala Gln Phe Ser Glu Leu Val
420 425 430
Asn Asp Tyr Tyr Asn Asn Gly Leu Pro Ser Asn Leu Thr Ala Ser Arg
435 440 445
Asn Pro Ser Leu Asp Tyr Gly Phe Lys Gly Ala Glu Ile Ala Met Ala
450 455 460
Ser Tyr Cys Ser Glu Leu Gln Tyr Leu Ala Asn Pro Val Thr Ser His
465 470 475 480
Val Gln Ser Ala Glu Gln His Asn Gln Asp Val Asn Ser Leu Gly Leu
485 490 495
Ile Ser Ser Arg Lys Thr His Glu Ala Ile Glu Ile Leu Lys Leu Met
500 505 510
Ser Ser Thr Phe Leu Val Ala Leu Cys Gln Ala Ile Asp Leu Arg His
515 520 525
Leu Glu Glu Asn Leu Lys Asn Thr Val Lys Asn Val Val Ser Gln Val
530 535 540
Ala Lys Arg Thr Leu Thr Thr Gly Val Asn Gly Glu Leu His Pro Ser
545 550 555 560
Arg Phe Cys Glu Lys Asp Leu Leu Lys Val Val Asp Arg Glu Tyr Thr
565 570 575
Phe Ala Tyr Ile Asp Asp Pro Cys Ser Gly Thr Tyr Pro Leu Met Gln
580 585 590
Lys Leu Arg Gln Val Leu Val Asp Tyr Ala Leu Ala Asn Gly Glu Asn
595 600 605
Glu Lys Asn Thr Ser Thr Ser Ile Phe Gln Lys Ile Ala Thr Phe Glu
610 615 620
Glu Glu Leu Lys Thr Leu Leu Pro Lys Glu Val Glu Gly Ala Arg Val
625 630 635 640
Ala Tyr Glu Asn Asp Gln Cys Ala Ile Pro Asn Lys Ile Lys Glu Cys
645 650 655
Arg Ser Tyr Pro Leu Tyr Lys Phe Val Arg Glu Glu Leu Gly Thr Ala
660 665 670
Leu Leu Thr Gly Glu Arg Val Ile Ser Pro Gly Glu Glu Cys Asp Lys
675 680 685
Val Phe Thr Ala Leu Cys Gln Gly Lys Ile Ile Asp Pro Leu Leu Glu
690 695 700
Cys Leu Gly Glu Trp Asn Gly Ala Pro Leu Pro Ile Cys
705 710 715
<210> 105
<211> 713
<212> PRT
<213> 饲用甜菜()
<220>
<223> 糖用甜菜苯丙氨酸氨裂合酶 (BvPAL)
<400> 105
Met Glu Cys Glu Asn Ala His Val Ala Ala Asn Gly Asp Gly Leu Cys
1 5 10 15
Val Ala Gln Pro Ala Arg Ala Asp Pro Leu Asn Trp Gly Lys Ala Ala
20 25 30
Glu Glu Leu Ser Gly Ser His Leu Asp Ala Val Lys Arg Met Val Glu
35 40 45
Glu Tyr Arg Lys Pro Val Val Thr Met Glu Gly Ala Ser Leu Thr Ile
50 55 60
Ala Met Val Ala Ala Val Ala Ala Gly Asn Asp Thr Arg Val Glu Leu
65 70 75 80
Asp Glu Ser Ala Arg Gly Arg Val Lys Glu Ser Ser Asp Trp Val Met
85 90 95
Asn Ser Met Met Asn Gly Thr Asp Ser Tyr Gly Val Thr Thr Gly Phe
100 105 110
Gly Ala Thr Ser His Arg Arg Thr Lys Glu Gly Gly Ala Leu Gln Arg
115 120 125
Glu Leu Ile Arg Phe Leu Asn Ala Gly Ala Phe Gly Thr Gly Thr Asp
130 135 140
Gly His Val Leu Pro Ala Ala Thr Thr Arg Ala Ala Met Leu Val Arg
145 150 155 160
Val Asn Thr Leu Leu Gln Gly Tyr Ser Gly Ile Arg Phe Glu Ile Leu
165 170 175
Glu Thr Ile Ala Thr Leu Leu Asn Ala Asn Val Thr Pro Cys Leu Pro
180 185 190
Leu Arg Gly Thr Ile Thr Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr
195 200 205
Ile Ala Gly Leu Val Thr Gly Arg Pro Asn Ser Val Ala Thr Ala Pro
210 215 220
Asp Gly Thr Lys Val Asn Ala Ala Glu Ala Phe Lys Ile Ala Gly Ile
225 230 235 240
Gln His Gly Phe Phe Glu Leu Gln Pro Lys Glu Gly Leu Ala Met Val
245 250 255
Asn Gly Thr Ala Val Gly Ser Gly Leu Ala Ser Met Val Leu Phe Glu
260 265 270
Ala Asn Ile Leu Ser Leu Leu Ala Glu Val Leu Ser Ala Val Phe Cys
275 280 285
Glu Val Met Asn Gly Lys Pro Glu Tyr Thr Asp His Leu Thr His Lys
290 295 300
Leu Lys His His Pro Gly Gln Ile Glu Ala Ala Ala Ile Met Glu His
305 310 315 320
Ile Leu Glu Gly Ser Ser Tyr Met Ile Leu Ala Lys Lys Leu Gly Glu
325 330 335
Leu Asp Pro Leu Met Lys Pro Lys Gln Asp Arg Tyr Ala Leu Arg Thr
340 345 350
Ser Pro Gln Trp Leu Gly Pro Gln Ile Glu Val Ile Arg Ala Ala Thr
355 360 365
Lys Ser Ile Glu Arg Glu Ile Asn Ser Val Asn Asp Asn Pro Leu Ile
370 375 380
Asp Val Ser Arg Gly Lys Ala Ile His Gly Gly Asn Phe Gln Gly Thr
385 390 395 400
Pro Ile Gly Val Ser Met Asp Asn Thr Arg Leu Ala Ile Ala Ala Ile
405 410 415
Gly Lys Leu Met Phe Ala Gln Phe Ser Glu Leu Val Asn Asp Phe Tyr
420 425 430
Asn Asn Gly Leu Pro Ser Asn Leu Ser Gly Gly Arg Asn Pro Ser Leu
435 440 445
Asp Tyr Gly Phe Lys Gly Ala Glu Ile Ala Met Ala Ser Tyr Cys Ser
450 455 460
Glu Leu Gln Phe Leu Gly Asn Pro Val Thr Asn His Val Gln Ser Ala
465 470 475 480
Glu Gln His Asn Gln Asp Val Asn Ser Leu Gly Leu Ile Ser Ser Arg
485 490 495
Lys Thr Ala Glu Ala Ile Asp Ile Leu Lys Leu Met Ser Ser Thr Phe
500 505 510
Leu Val Ala Leu Cys Gln Ala Ile Asp Leu Arg His Leu Glu Glu Asn
515 520 525
Val Lys Asn Ala Val Lys Ser Cys Val Lys Thr Val Ala Arg Lys Thr
530 535 540
Leu Ser Thr Asp Asn Asn Gly His Leu His Lys Ala Arg Phe Cys Glu
545 550 555 560
Lys Asp Leu Leu Leu Thr Ile Asp Arg Glu Ala Val Phe Ala Tyr Ala
565 570 575
Asp Asp Pro Cys Ser Val Asn Tyr Pro Leu Met Gln Lys Met Arg Ala
580 585 590
Val Leu Val Glu His Ala Leu Ala Asn Gly Glu Ala Glu His Asp Val
595 600 605
Glu Thr Ser Val Phe Ala Lys Leu Ala Thr Phe Glu Gln Glu Leu Arg
610 615 620
Ala Val Leu Pro Thr Glu Val Glu Ala Ala Arg Ser Ala Val Glu Asn
625 630 635 640
Gly Thr Ala Ala Gln Gln Asn Arg Ile Ser Glu Cys Arg Ser Tyr Pro
645 650 655
Leu Tyr Arg Phe Val Arg Lys Glu Leu Gly Thr Glu Tyr Leu Thr Gly
660 665 670
Glu Lys Thr Arg Ser Pro Gly Glu Glu Val Asp Lys Val Phe Val Ala
675 680 685
Met Asn Gln Gly Lys His Ile Asp Ala Leu Leu Glu Cys Leu Lys Glu
690 695 700
Trp Asn Gly Glu Pro Leu Pro Ile Cys
705 710
<210> 106
<211> 715
<212> PRT
<213> 红花烟草()
<220>
<223> 烟草苯丙氨酸氨裂合酶 (NtPAL1)
<400> 106
Met Ala Ser Asn Gly His Val Asn Gly Gly Glu Asn Phe Glu Leu Cys
1 5 10 15
Lys Lys Ser Ala Asp Pro Leu Asn Trp Glu Met Ala Ala Glu Ser Leu
20 25 30
Arg Gly Ser His Leu Asp Glu Val Lys Lys Met Val Ser Glu Phe Arg
35 40 45
Lys Pro Met Val Lys Leu Gly Gly Glu Ser Leu Thr Val Ala Gln Val
50 55 60
Ala Ala Ile Ala Val Arg Asp Lys Ser Ala Asn Gly Val Lys Val Glu
65 70 75 80
Leu Ser Glu Glu Ala Arg Ala Gly Val Lys Ala Ser Ser Asp Trp Val
85 90 95
Met Asp Ser Met Asn Lys Gly Thr Asp Ser Tyr Gly Val Thr Thr Gly
100 105 110
Phe Gly Ala Thr Ser His Arg Arg Thr Lys Asn Gly Gly Ala Leu Gln
115 120 125
Lys Glu Leu Ile Arg Phe Leu Asn Ala Gly Val Phe Gly Asn Gly Thr
130 135 140
Glu Thr Ser His Thr Leu Pro His Ser Ala Thr Arg Ala Ala Met Leu
145 150 155 160
Val Arg Ile Asn Thr Leu Leu Gln Gly Tyr Ser Gly Ile Arg Phe Glu
165 170 175
Ile Leu Glu Ala Ile Thr Lys Leu Ile Asn Ser Asn Ile Thr Pro Cys
180 185 190
Leu Pro Leu Arg Gly Thr Ile Thr Ala Ser Gly Asp Leu Val Pro Leu
195 200 205
Ser Tyr Ile Ala Gly Leu Leu Thr Gly Arg Pro Asn Ser Lys Ala Val
210 215 220
Gly Pro Asn Gly Glu Thr Leu Asn Ala Glu Glu Ala Phe Arg Val Ala
225 230 235 240
Gly Val Asn Gly Gly Phe Phe Glu Leu Gln Pro Lys Glu Gly Leu Ala
245 250 255
Leu Val Asn Gly Thr Ala Val Gly Ser Gly Met Ala Ser Met Val Leu
260 265 270
Phe Asp Ser Asn Ile Leu Ala Val Met Ser Glu Val Leu Ser Ala Ile
275 280 285
Phe Ala Glu Val Met Asn Gly Lys Pro Glu Phe Thr Asp His Leu Thr
290 295 300
His Lys Leu Lys His His Pro Gly Gln Ile Glu Ala Ala Ala Ile Met
305 310 315 320
Glu His Ile Leu Asp Gly Ser Ser Tyr Val Lys Ala Ala Gln Lys Leu
325 330 335
His Glu Met Asp Pro Leu Gln Lys Pro Lys Gln Asp Arg Tyr Ala Leu
340 345 350
Arg Thr Ser Pro Gln Trp Leu Gly Pro Gln Ile Glu Val Ile Arg Ala
355 360 365
Ala Thr Lys Met Ile Glu Arg Glu Ile Asn Ser Val Asn Asp Asn Pro
370 375 380
Leu Ile Asp Val Ser Arg Asn Lys Ala Leu His Gly Gly Asn Phe Gln
385 390 395 400
Gly Thr Pro Ile Gly Val Ser Met Asp Asn Ala Arg Leu Ala Leu Ala
405 410 415
Ser Ile Gly Lys Leu Met Phe Ala Gln Phe Ser Glu Leu Val Asn Asp
420 425 430
Tyr Tyr Asn Asn Gly Leu Pro Ser Asn Leu Thr Ala Ser Arg Asn Pro
435 440 445
Ser Leu Asp Tyr Gly Phe Lys Gly Ala Glu Ile Ala Met Ala Ser Tyr
450 455 460
Cys Ser Glu Leu Gln Phe Leu Ala Asn Pro Val Thr Asn His Val Gln
465 470 475 480
Ser Ala Glu Gln His Asn Gln Asp Val Asn Ser Leu Gly Leu Ile Ser
485 490 495
Ala Arg Lys Thr Ala Glu Ala Val Asp Ile Leu Lys Leu Met Ser Ser
500 505 510
Thr Tyr Leu Val Ala Leu Cys Gln Ala Ile Asp Leu Arg His Leu Glu
515 520 525
Glu Asn Leu Lys Asn Ala Val Lys Asn Thr Val Ser Gln Val Ala Lys
530 535 540
Arg Thr Leu Thr Met Gly Ala Asn Gly Glu Leu His Pro Ala Arg Phe
545 550 555 560
Cys Glu Lys Glu Leu Leu Arg Ile Val Asp Arg Glu Tyr Leu Phe Ala
565 570 575
Tyr Ala Asp Asp Pro Cys Ser Cys Asn Tyr Pro Leu Met Gln Lys Leu
580 585 590
Arg Gln Val Leu Val Asp His Ala Met Asn Asn Gly Glu Ser Glu Lys
595 600 605
Asn Val Asn Ser Ser Ile Phe Gln Lys Ile Gly Ala Phe Glu Asp Glu
610 615 620
Leu Lys Ala Val Leu Pro Lys Glu Val Glu Ser Ala Arg Ala Ala Leu
625 630 635 640
Glu Ser Gly Asn Pro Ala Ile Pro Asn Arg Ile Thr Glu Cys Arg Ser
645 650 655
Tyr Pro Leu Tyr Arg Phe Val Arg Lys Glu Leu Gly Thr Glu Leu Leu
660 665 670
Thr Gly Glu Lys Val Arg Ser Pro Gly Glu Glu Cys Asp Lys Val Phe
675 680 685
Thr Ala Met Cys Asn Gly Gln Ile Ile Asp Pro Met Leu Glu Cys Leu
690 695 700
Lys Ser Trp Asn Gly Ala Pro Leu Pro Ile Cys
705 710 715
<210> 107
<211> 720
<212> PRT
<213> 马铃薯()
<220>
<223> 马铃薯苯丙氨酸氨裂合酶 (StPAL1)
<400> 107
Met Ala Pro Ser Ile Ala Gln Asn Gly His Val Asn Gly Glu Val Glu
1 5 10 15
Glu Val Leu Trp Lys Lys Ser Ile His Asp Pro Leu Asn Trp Glu Met
20 25 30
Ala Val Asp Ser Leu Arg Gly Ser His Leu Asp Glu Val Lys Lys Met
35 40 45
Val Asp Glu Phe Arg Lys Pro Ile Val Lys Leu Trp Gly Glu Thr Leu
50 55 60
Thr Val Ala Gln Val Ala Ser Ile Ala Asn Ala Asp Asn Lys Thr Ser
65 70 75 80
Gly Phe Lys Val Glu Leu Ser Glu Ser Ala Arg Ala Gly Val Lys Ala
85 90 95
Ser Ser Asp Trp Val Met Asp Ser Met Ser Lys Gly Thr Asp Ser Tyr
100 105 110
Gly Val Thr Thr Gly Phe Cys Ala Thr Ser His Arg Arg Thr Lys Asn
115 120 125
Gly Gly Ala Leu Gln Lys Glu Leu Ile Lys Phe Leu Asn Ala Gly Val
130 135 140
Phe Gly Asn Gly Thr Glu Ser Thr His Thr Leu Pro His Ser Ala Thr
145 150 155 160
Arg Ala Ala Met Leu Val Arg Ile Asn Thr Leu Leu Gln Gly Tyr Ser
165 170 175
Gly Ile Arg Phe Glu Ile Leu Glu Ala Ile Thr Lys Leu Ile Asn Ser
180 185 190
Asn Ile Thr Pro Cys Leu Pro Leu Arg Gly Thr Val Thr Ala Ser Gly
195 200 205
Asp Leu Val Pro Leu Ser Tyr Ile Ala Gly Leu Leu Thr Gly Arg Pro
210 215 220
Asn Ser Lys Ala Val Gly Pro Ser Gly Ser Lys Leu Asp Ala Asp Glu
225 230 235 240
Ala Phe Arg Val Ala Ala Val Ser Gly Gly Phe Phe Glu Leu Gln Pro
245 250 255
Lys Glu Gly Leu Ala Leu Val Asn Gly Thr Ala Val Gly Ser Gly Met
260 265 270
Ala Ser Ile Val Leu Tyr Asp Ser Asn Ile Leu Ala Val Met Phe Glu
275 280 285
Val Leu Ser Ala Ile Phe Ala Glu Val Met Asn Gly Lys Pro Glu Phe
290 295 300
Thr Asp Tyr Leu Thr His Lys Leu Lys His His Pro Gly Gln Ile Glu
305 310 315 320
Ala Ala Ala Ile Met Glu His Ile Leu Asp Gly Ser Ser Tyr Val Lys
325 330 335
Ala Ala Gln Lys Leu His Glu Met Asp Pro Leu Gln Lys Pro Lys Gln
340 345 350
Asp Arg Tyr Ala Leu Arg Thr Ser Pro Gln Trp Leu Gly Pro Gln Ile
355 360 365
Glu Val Ile Arg Ala Ala Thr Lys Met Ile Glu Arg Glu Ile Asn Ser
370 375 380
Val Asn Asp Asn Pro Leu Ile Asp Val Ser Arg Asn Lys Ala Ile His
385 390 395 400
Gly Gly Asn Phe Gln Gly Thr Pro Ile Gly Val Ser Met Asp Asn Thr
405 410 415
Arg Leu Ala Leu Ala Ser Ile Gly Lys Leu Met Phe Ala Gln Phe Ser
420 425 430
Glu Leu Val Asn Asp Tyr Tyr Asn Asn Gly Leu Pro Ser Asn Leu Thr
435 440 445
Ala Gly Arg Asn Pro Ser Leu Asp Tyr Gly Phe Lys Gly Ala Glu Ile
450 455 460
Ala Met Ala Ser Tyr Cys Ser Glu Leu Gln Phe Leu Ala Asn Pro Val
465 470 475 480
Thr Asn His Val Gln Ser Ala Glu Gln His Asn Gln Asp Val Asn Ser
485 490 495
Leu Gly Leu Ile Ser Ala Arg Lys Thr Ala Glu Ala Val Asp Ile Leu
500 505 510
Lys Leu Met Ser Ser Thr Tyr Leu Val Ala Leu Cys Gln Ala Ile Asp
515 520 525
Leu Arg His Leu Glu Glu Asn Leu Lys Ser Val Val Lys Asn Thr Val
530 535 540
Ser Gln Val Ala Lys Arg Thr Leu Thr Ile Gly Ala Ile Gly Glu Leu
545 550 555 560
His Pro Ala Arg Phe Cys Glu Lys Glu Leu Leu Arg Val Val Asp Arg
565 570 575
Glu Tyr Leu Phe Thr Tyr Ala Asp Asp Pro Cys Ser Ser Thr Tyr Pro
580 585 590
Leu Met Gln Lys Leu Arg Gln Val Leu Val Asp His Ala Met Lys Asn
595 600 605
Gly Glu Ser Glu Lys Asn Ile Asn Ser Ser Ile Phe Gln Lys Ile Gly
610 615 620
Ala Phe Glu Asp Glu Leu Asn Ala Val Leu Pro Lys Glu Val Glu Ser
625 630 635 640
Ala Arg Ala Leu Leu Glu Ser Gly Asn Pro Ser Ile Pro Asn Arg Ile
645 650 655
Thr Glu Cys Arg Ser Tyr Pro Leu Tyr Arg Leu Val Arg Gln Glu Leu
660 665 670
Gly Thr Glu Leu Leu Thr Gly Glu Lys Val Arg Ser Pro Gly Glu Glu
675 680 685
Ile Glu Lys Val Phe Thr Ala Met Cys Asn Gly Gln Ile Asn Asp Pro
690 695 700
Leu Leu Glu Cys Leu Lys Ser Trp Asn Gly Ala Pro Leu Pro Ile Cys
705 710 715 720
<210> 108
<211> 712
<212> PRT
<213> 绿竹()
<220>
<223> 竹苯丙氨酸氨裂合酶 (BoPAL)
<400> 108
Met Pro Arg Glu Asp Gly His Val Ala Ala Asn Gly Asn Gly Leu Cys
1 5 10 15
Met Ala Ala Pro Arg Ala Asp Pro Leu Asn Trp Gly Lys Ala Ala Glu
20 25 30
Glu Leu Met Gly Ser His Leu Asp Glu Val Lys Arg Met Val Ala Glu
35 40 45
Tyr Arg Gln Pro Val Val Lys Ile Glu Gly Ala Ser Leu Arg Ile Ala
50 55 60
Gln Val Ala Ala Val Ala Val Ala Gly Asp Ala Lys Val Glu Leu Asp
65 70 75 80
Glu Ser Ala Arg Glu Arg Val Lys Ala Ser Ser Asp Trp Val Met Asn
85 90 95
Ser Met Met Asn Gly Thr Asp Ser Tyr Gly Val Thr Thr Gly Phe Gly
100 105 110
Ala Thr Ser His Arg Arg Thr Lys Glu Gly Gly Ala Leu Gln Arg Glu
115 120 125
Leu Ile Arg Phe Leu Asn Ala Gly Ala Phe Gly Thr Gly Cys Asp Gly
130 135 140
His Val Leu Pro Ala Glu Ala Thr Arg Ala Ala Met Leu Val Arg Ile
145 150 155 160
Asn Thr Leu Leu Gln Gly Tyr Ser Gly Ile Arg Phe Glu Ile Leu Glu
165 170 175
Ala Ile Thr Lys Leu Leu Asn Ala Asn Val Thr Pro Cys Leu Pro Leu
180 185 190
Arg Gly Thr Val Thr Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr Ile
195 200 205
Ala Gly Leu Val Thr Gly Arg Glu Asn Ser Val Ala Val Ala Pro Asp
210 215 220
Gly Arg Lys Val Asn Ala Ala Glu Ala Phe Lys Ile Ala Gly Ile Gln
225 230 235 240
Gly Gly Phe Phe Glu Leu Gln Pro Lys Glu Gly Leu Ala Met Val Asn
245 250 255
Gly Thr Ala Val Gly Ser Gly Leu Ala Ser Thr Val Leu Phe Glu Ala
260 265 270
Asn Ile Leu Ala Ile Leu Ala Glu Val Leu Ser Ala Val Phe Cys Glu
275 280 285
Val Met Asn Gly Lys Pro Glu Tyr Thr Asp His Leu Thr His Lys Leu
290 295 300
Lys His His Pro Gly Gln Ile Glu Ala Ala Ala Ile Met Glu His Ile
305 310 315 320
Leu Glu Gly Ser Ser Tyr Met Lys Leu Ala Lys Lys Leu Gly Asp Leu
325 330 335
Asp Pro Leu Met Lys Pro Lys Gln Asp Arg Tyr Ala Leu Arg Thr Ser
340 345 350
Pro Gln Trp Leu Gly Pro Gln Ile Glu Val Ile Arg Ala Ala Thr Lys
355 360 365
Ser Ile Glu Arg Glu Ile Asn Ser Val Asn Asp Asn Pro Leu Ile Asp
370 375 380
Val Ser Arg Asn Lys Ala Leu His Gly Gly Asn Phe Gln Gly Thr Pro
385 390 395 400
Ile Gly Val Ser Met Asp Asn Thr Arg Leu Ala Ile Ala Ala Ile Gly
405 410 415
Lys Leu Met Phe Ala Gln Phe Ser Glu Leu Val Asn Asp Phe Tyr Asn
420 425 430
Asn Gly Leu Pro Ser Asn Leu Ser Gly Gly Arg Asn Pro Ser Leu Asp
435 440 445
Tyr Gly Phe Lys Gly Ala Glu Ile Ala Met Ala Ser Tyr Cys Ser Glu
450 455 460
Leu Gln Phe Leu Gly Asn Pro Val Thr Asn His Val Gln Ser Ala Glu
465 470 475 480
Gln His Asn Gln Asp Val Asn Ser Leu Gly Leu Ile Ser Ser Arg Lys
485 490 495
Thr Ala Glu Ala Ile Asp Ile Leu Lys Ile Met Ser Ser Thr Phe Leu
500 505 510
Val Ala Leu Cys Gln Ala Ile Asp Leu Arg His Ile Glu Glu Asn Val
515 520 525
Lys Ser Ala Val Lys Ser Cys Val Met Thr Val Ala Lys Lys Thr Pro
530 535 540
Ser Thr Asn Ser Thr Gly Asp Leu His Val Ala Arg Phe Cys Glu Lys
545 550 555 560
Asp Leu Leu Lys Glu Ile Asp Arg Glu Ala Val Phe Ala Tyr Ala Asp
565 570 575
Asp Pro Cys Ser Pro Asn Tyr Pro Leu Met Lys Lys Leu Arg Ser Val
580 585 590
Leu Val Glu Ser Ala Leu Ala Asn Gly Met Ala Glu Phe Asn Ala Glu
595 600 605
Thr Ser Ile Phe Ala Arg Val Ala Leu Phe Glu Glu Glu Leu Arg Ala
610 615 620
Ala Leu Pro Arg Ala Val Glu Ala Ala Arg Ala Ser Val Glu Asn Gly
625 630 635 640
Thr Ala Ala Ala Pro Asn Arg Ile Thr Glu Cys Arg Ser Tyr Pro Leu
645 650 655
Tyr Arg Phe Val Arg Glu Glu Leu Gly Thr Glu Tyr Leu Thr Gly Glu
660 665 670
Lys Thr Arg Ser Pro Gly Glu Glu Leu Asn Lys Val Leu Leu Ala Ile
675 680 685
Asn Gln Gly Lys His Ile Asp Pro Leu Leu Glu Cys Leu Lys Glu Trp
690 695 700
Asn Gly Glu Pro Leu Pro Ile Cys
705 710
<210> 109
<211> 722
<212> PRT
<213> 芫菁()
<220>
<223> 苯丙氨酸氨裂合酶 (BnPAL1)
<400> 109
Met Glu Val Asn Gly Leu Ser His Gly Gly Glu Val Asp Ala Met Leu
1 5 10 15
Cys Gly Gly Glu Ile Lys Lys Asn Ala Thr Val Val Gly Ala Asp Pro
20 25 30
Leu Asn Trp Gly Ala Ala Ala Glu Gln Met Lys Gly Ser His Leu Asp
35 40 45
Glu Val Lys Arg Met Val Lys Glu Phe Arg Arg Pro Val Val Asn Leu
50 55 60
Gly Gly Glu Thr Leu Thr Ile Gly Gln Val Ala Ala Ile Ser Thr Leu
65 70 75 80
Gly Asn Gly Val Lys Val Glu Leu Ser Glu Thr Ala Arg Ala Gly Val
85 90 95
Lys Ala Ser Ser Asp Trp Val Met Glu Ser Met Gly Lys Gly Thr Asp
100 105 110
Ser Tyr Gly Val Thr Thr Gly Phe Gly Ala Thr Ser His Arg Arg Thr
115 120 125
Lys Asn Gly Val Ala Leu Gln Lys Glu Leu Ile Arg Phe Leu Asn Ala
130 135 140
Gly Ile Phe Gly Ser Thr Lys Glu Thr Ser His Thr Leu Pro His Ser
145 150 155 160
Ala Thr Arg Ala Ala Met Leu Val Arg Ile Asn Thr Leu Leu Gln Gly
165 170 175
Tyr Ser Gly Ile Arg Phe Glu Ile Leu Glu Ala Ile Thr Ser Phe Leu
180 185 190
Asn Asn Asn Ile Thr Pro Ser Leu Pro Leu Arg Gly Thr Ile Thr Ala
195 200 205
Ser Gly Asp Leu Val Pro Leu Ser Tyr Ile Ala Gly Leu Leu Thr Gly
210 215 220
Arg Pro Asn Ser Lys Ala Thr Gly Pro Asn Gly Glu Ala Leu Asn Ala
225 230 235 240
Glu Glu Ala Phe Lys Met Ala Gly Val Thr Ser Gly Phe Phe Asp Leu
245 250 255
Gln Pro Lys Glu Gly Leu Ala Leu Val Asn Gly Thr Ala Val Gly Ser
260 265 270
Gly Met Ala Ser Met Val Leu Phe Glu Ala Asn Val Leu Ser Val Leu
275 280 285
Ala Glu Val Leu Ser Ala Val Phe Ala Glu Val Met Ser Gly Lys Pro
290 295 300
Glu Phe Thr Asp His Leu Thr His Arg Leu Lys His His Pro Gly Gln
305 310 315 320
Ile Glu Ala Ala Ala Ile Met Glu His Ile Leu Asp Gly Ser Ser Tyr
325 330 335
Met Lys Leu Ala Gln Lys Leu His Glu Met Asp Pro Leu Gln Lys Pro
340 345 350
Lys Gln Asp Arg Tyr Ala Leu Arg Thr Ser Pro Gln Trp Leu Gly Pro
355 360 365
Gln Ile Glu Val Ile Arg Tyr Ala Thr Lys Ser Ile Glu Arg Glu Ile
370 375 380
Asn Ser Val Asn Asp Asn Pro Leu Ile Asp Val Ser Arg Asn Lys Ala
385 390 395 400
Ile His Gly Gly Asn Phe Gln Gly Thr Pro Ile Gly Val Ser Met Asp
405 410 415
Asn Thr Arg Leu Ala Ile Ala Ala Ile Gly Lys Leu Met Phe Ala Gln
420 425 430
Phe Ser Glu Leu Val Asn Asp Tyr Tyr Asn Asn Gly Leu Pro Ser Asn
435 440 445
Leu Thr Ala Ser Arg Asn Pro Ser Leu Asp Tyr Gly Phe Lys Gly Ala
450 455 460
Glu Ile Ala Met Ala Ser Tyr Cys Ser Glu Leu Gln Tyr Leu Ala Asn
465 470 475 480
Pro Val Thr Ser His Val Gln Ser Ala Glu Gln His Asn Gln Asp Val
485 490 495
Asn Ser Leu Gly Leu Ile Ser Ser Arg Lys Thr Ser Glu Ala Val Asp
500 505 510
Ile Leu Lys Leu Met Ser Thr Thr Phe Leu Val Gly Ile Cys Gln Ala
515 520 525
Ile Asp Leu Arg His Leu Glu Glu Asn Leu Lys Gln Thr Val Lys Asn
530 535 540
Thr Val Ser Gln Val Ala Lys Lys Val Leu Thr Thr Gly Val Asn Gly
545 550 555 560
Glu Leu His Pro Ser Arg Phe Cys Glu Lys Asp Leu Leu Lys Val Val
565 570 575
Asp Arg Glu Gln Val Tyr Thr Tyr Ala Asp Asp Pro Cys Ser Ala Thr
580 585 590
Tyr Pro Leu Ile Gln Lys Leu Arg Gln Val Ile Val Asp His Ala Leu
595 600 605
Val Asn Gly Glu Ser Glu Lys Asn Ala Met Thr Ser Ile Phe His Lys
610 615 620
Ile Gly Ala Phe Glu Glu Glu Leu Lys Ala Val Leu Pro Lys Glu Val
625 630 635 640
Glu Ala Ala Arg Ala Ala Tyr Asp Asn Gly Thr Ala Ala Ile Pro Asn
645 650 655
Arg Ile Lys Glu Cys Arg Ser Tyr Pro Leu Tyr Arg Phe Val Arg Glu
660 665 670
Glu Leu Gly Thr Glu Leu Leu Thr Gly Glu Lys Ala Thr Ser Pro Gly
675 680 685
Glu Glu Phe Asp Lys Val Phe Thr Ala Ile Cys Glu Gly Lys Ile Ile
690 695 700
Gly Pro Leu Met Glu Cys Leu Asp Glu Trp Asn Gly Ala Pro Ile Pro
705 710 715 720
Ile Cys
<210> 110
<211> 667
<212> PRT
<213> 向日葵()
<220>
<223> 向日葵苯丙氨酸氨裂合酶 (HaPAL)
<400> 110
Met Glu Asn Gly Thr His Val Asn Gly Ser Ala Asn Gly Phe Cys Ile
1 5 10 15
Lys Asp Pro Leu Asn Trp Gly Val Ala Ala Glu Ala Leu Thr Gly Ser
20 25 30
His Leu Asp Glu Val Lys Lys Met Val Gly Glu Phe Arg Lys Pro Val
35 40 45
Val Lys Leu Gly Gly Glu Thr Leu Thr Val Ser Gln Val Ala Gly Ile
50 55 60
Ser Ala Ala Gly Asp Gly Asn Met Val Lys Val Glu Leu Ser Glu Ala
65 70 75 80
Ala Arg Ala Gly Val Lys Ala Ser Ser Asp Trp Val Met Glu Ser Met
85 90 95
Asn Lys Gly Thr Asp Ser Tyr Gly Val Thr Thr Gly Phe Gly Ala Thr
100 105 110
Ser His Arg Arg Thr Lys Asn Gly Gly Ala Leu Gln Lys Glu Leu Ile
115 120 125
Arg Phe Leu Asn Ala Gly Ile Phe Gly Asn Gly Thr Glu Ser Ser His
130 135 140
Thr Leu Pro His Ser Ala Thr Arg Ala Ala Met Leu Val Arg Ile Asn
145 150 155 160
Thr Leu Leu Gln Gly Tyr Ser Gly Ile Arg Phe Glu Ile Leu Glu Ala
165 170 175
Ile Thr Lys Phe Leu Asn Asn Asn Ile Thr Pro Cys Leu Pro Leu Arg
180 185 190
Gly Thr Ile Thr Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr Ile Ala
195 200 205
Gly Leu Leu Thr Gly Arg Pro Asn Ser Lys Ala Val Gly Pro Ala Gly
210 215 220
Glu Val Leu Asn Ala Glu Ser Ala Phe Ala Gln Ala Gly Val Glu Gly
225 230 235 240
Gly Phe Phe Glu Leu Gln Pro Lys Glu Gly Leu Ala Leu Val Asn Gly
245 250 255
Thr Ala Val Gly Ser Gly Met Ala Ser Met Val Leu Phe Glu Ala Asn
260 265 270
Val Leu Ala Leu Leu Ser Glu Val Leu Ser Ala Ile Phe Ala Glu Val
275 280 285
Met Gln Gly Lys Pro Glu Phe Thr Asp His Leu Thr His Lys Leu Lys
290 295 300
His His Pro Gly Gln Ile Glu Ala Ala Ala Ile Met Glu Tyr Ile Leu
305 310 315 320
Asp Gly Ser Asp Tyr Val Lys Ala Ala Gln Lys Val His Glu Met Asp
325 330 335
Pro Leu Gln Lys Pro Lys Gln Asp Arg Tyr Ala Leu Arg Thr Ser Pro
340 345 350
Gln Trp Leu Gly Pro Gln Ile Glu Val Ile Arg Ser Ala Thr Lys Met
355 360 365
Ile Glu Arg Glu Ile Asn Ser Val Asn Asp Asn Pro Leu Ile Asp Val
370 375 380
Ser Arg Asn Lys Ala Leu His Gly Gly Asn Phe Gln Gly Thr Pro Ile
385 390 395 400
Gly Val Ser Met Asp Asn Thr Arg Leu Ala Ile Ala Ala Ile Gly Lys
405 410 415
Val Thr Ile Ala Gln Phe Ser Glu Leu Val Asn Asp Phe Tyr Asn Asn
420 425 430
Gly Leu Pro Ser His Leu Ser Gly Gly Arg Asn Pro Ser Leu Asp Ser
435 440 445
Gly Phe Lys Gly Gly Glu Ile Ala Met Ala Ser Tyr Cys Ser Glu Leu
450 455 460
Gln Phe Leu Ala Asn Pro Val Thr Asn His Val Gln Ser Ala Glu Gln
465 470 475 480
His Asn Gln Asp Val Asn Ser Leu Gly Leu Ile Ser Ala Arg Lys Thr
485 490 495
Ala Glu Ala Val Asp Ile Leu Lys Leu Met Ser Ser Thr Tyr Leu Val
500 505 510
Ala Leu Cys Gln Ser Ile Asp Leu Arg His Leu Glu Glu Asn Met Lys
515 520 525
Ser Thr Val Lys Asn Thr Val Ser Gln Val Ala Lys Lys Val Leu Thr
530 535 540
Met Gly Val Asn Gly Glu Leu His Pro Ser Arg Phe Cys Glu Lys Asp
545 550 555 560
Leu Leu Arg Val Val Asp Arg Glu Tyr Val Phe Ala Tyr Ala Asp Asp
565 570 575
Pro Cys Leu Thr Thr Tyr Pro Leu Met Gln Lys Leu Arg Gln Val Leu
580 585 590
Val Asp His Ala Leu Asn Asn Gly Glu Thr Glu Lys Asn Ala Asn Thr
595 600 605
Ser Ile Phe Gln Lys Ile Ala Thr Phe Glu Asp Glu Leu Lys Ala Ile
610 615 620
Leu Pro Lys Glu Val Glu Ser Val Arg Val Ala Phe Glu Asn Gly Thr
625 630 635 640
Met Ser Ile Pro Asn Arg Ile Lys Ala Cys Arg Ser Tyr Pro Leu Tyr
645 650 655
Arg Phe Val Arg Glu Glu Leu Gly Gly Ala Thr
660 665
<210> 111
<211> 714
<212> PRT
<213> 蓖麻()
<220>
<223> 苯丙氨酸氨裂合酶 (RcPAL)
<400> 111
Met Ala Ala Met Ala Glu Asn Gly Ser Lys Asn Asp Ser Leu Glu Ser
1 5 10 15
Phe Cys Asn Met Gly Arg Asp Pro Leu Ser Trp Gly Leu Ala Ala Glu
20 25 30
Ser Met Lys Gly Ser His Leu Asp Glu Val Lys Lys Met Val Ala Glu
35 40 45
Tyr Arg Lys Pro Phe Val Lys Leu Gly Gly Glu Thr Leu Thr Val Ala
50 55 60
Gln Val Ala Ala Ile Ala Ser His Asp Cys Gly Val Lys Val Glu Leu
65 70 75 80
Ser Glu Ser Ala Arg Ala Gly Val Lys Ala Ser Ser Asp Trp Val Met
85 90 95
Asp Ser Met Asn Lys Gly Thr Asp Ser Tyr Gly Val Thr Thr Gly Phe
100 105 110
Gly Ala Thr Ser His Arg Arg Thr Lys Gln Gly Gly Ala Leu Gln Lys
115 120 125
Glu Leu Ile Arg Phe Leu Asn Ala Gly Ile Phe Gly Asn Gly Thr Glu
130 135 140
Ser Cys His Thr Leu Pro His Ser Ala Thr Arg Ala Ala Met Leu Val
145 150 155 160
Arg Ile Asn Thr Leu Leu Gln Gly Tyr Ser Gly Ile Arg Phe Glu Ile
165 170 175
Leu Glu Ala Ile Thr Lys Leu Leu Asn His Asn Ile Thr Pro Cys Leu
180 185 190
Pro Leu Arg Gly Thr Ile Thr Ala Ser Gly Asp Leu Val Pro Leu Ser
195 200 205
Tyr Ile Ala Gly Leu Leu Thr Gly Arg Pro Asn Ser Lys Ala Ile Gly
210 215 220
Pro Asn Gly Glu Ser Met Asp Ala Leu Glu Ala Phe Arg Leu Ala Gly
225 230 235 240
Ile Glu Ser Gly Phe Phe Glu Leu Gln Pro Lys Glu Gly Leu Ala Leu
245 250 255
Val Asn Gly Thr Ala Val Gly Ser Gly Leu Ala Ser Met Val Leu Phe
260 265 270
Glu Ala Asn Ile Leu Ala Val Leu Ser Glu Ile Leu Ser Ala Ile Phe
275 280 285
Ala Glu Val Met Asn Gly Lys Pro Glu Phe Thr Asp His Leu Thr His
290 295 300
Lys Leu Lys His His Pro Gly Gln Ile Glu Ala Ala Ala Ile Met Glu
305 310 315 320
His Ile Leu Asp Gly Ser Ser Tyr Val Lys Ala Ala Lys Lys Leu His
325 330 335
Glu Met Asp Pro Leu Gln Lys Pro Lys Gln Asp Arg Tyr Ala Leu Arg
340 345 350
Thr Ser Pro Gln Trp Leu Gly Pro Gln Ile Glu Val Ile Arg Phe Ser
355 360 365
Thr Lys Ser Ile Glu Arg Glu Ile Asn Ser Val Asn Asp Asn Pro Leu
370 375 380
Ile Asp Val Ser Arg Asn Lys Ala Leu His Gly Gly Asn Phe Gln Gly
385 390 395 400
Thr Pro Ile Gly Val Ser Met Asp Asn Ala Arg Leu Ala Ile Ala Ser
405 410 415
Ile Gly Lys Leu Met Phe Ala Gln Phe Ser Glu Leu Val Asn Asp Phe
420 425 430
Tyr Asn Asn Gly Leu Pro Ser Asn Leu Thr Ala Gly Arg Asn Pro Ser
435 440 445
Leu Asp Tyr Gly Phe Lys Gly Ala Glu Ile Ala Met Ala Ser Tyr Cys
450 455 460
Ser Glu Leu Gln Tyr Leu Ala Asn Pro Val Thr Ser His Val Gln Ser
465 470 475 480
Ala Glu Gln His Asn Gln Asp Val Asn Ser Leu Gly Leu Ile Ser Ser
485 490 495
Arg Lys Thr Ala Glu Ala Val Asp Ile Leu Lys Leu Met Ser Thr Thr
500 505 510
Tyr Leu Val Ala Leu Cys Gln Ala Ile Asp Leu Arg His Leu Glu Glu
515 520 525
Asn Leu Arg Gln Ala Val Lys Asn Thr Val Ser Gln Val Ala Lys Arg
530 535 540
Val Leu Thr Thr Gly Ala Asn Gly Glu Leu His Pro Ser Arg Phe Cys
545 550 555 560
Glu Lys Asp Leu Leu Lys Val Val Asp Arg Glu Tyr Val Phe Ala Tyr
565 570 575
Ala Asp Asp Pro Cys Ser Ala Thr Tyr Pro Leu Met Gln Lys Leu Arg
580 585 590
Gln Ile Leu Val Glu His Ala Leu Ala Asn Gly Glu Asn Glu Lys Asn
595 600 605
Ala Gly Thr Ser Val Phe Gln Lys Ile Ser Ala Phe Glu Glu Glu Leu
610 615 620
Lys Thr Leu Leu Pro Lys Glu Val Glu Ser Val Arg Ile Ala Tyr Glu
625 630 635 640
Ser Gly Asn Pro Ala Thr Ala Asn Arg Ile Lys Glu Cys Arg Ser Tyr
645 650 655
Pro Leu Tyr Lys Phe Val Arg Glu Glu Leu Gly Thr Gly Leu Leu Thr
660 665 670
Gly Asp Lys Val Met Ser Pro Gly Glu Glu Phe Asp Lys Val Phe Thr
675 680 685
Ala Met Cys Gln Gly Lys Ile Ile Asp Pro Met Met Asp Cys Leu Lys
690 695 700
Glu Trp Asn Gly Ala Pro Leu Pro Ile Cys
705 710
<210> 112
<211> 710
<212> PRT
<213> 葡萄()
<220>
<223> 葡萄苯丙氨酸氨裂合酶 (VvPAL)
<400> 112
Met Asp Ala Thr Asn Cys His Gly Ser Asn Lys Val Glu Ser Phe Cys
1 5 10 15
Val Ser Asp Pro Leu Asn Trp Gly Met Ala Ala Glu Thr Leu Lys Gly
20 25 30
Ser His Leu Asp Glu Val Lys Arg Met Val Ala Glu Tyr Arg Lys Pro
35 40 45
Val Val Arg Leu Gly Gly Glu Thr Leu Thr Ile Ser Gln Val Ala Ala
50 55 60
Ile Ala Gly Arg Glu Gly Asp Val Gly Val Glu Leu Ser Glu Thr Ala
65 70 75 80
Arg Ala Gly Val Asn Ala Ser Ser Glu Trp Val Met Glu Ser Met Ser
85 90 95
Lys Gly Thr Asp Ser Tyr Gly Val Thr Thr Gly Phe Gly Ala Thr Ser
100 105 110
His Arg Arg Thr Lys Gln Gly Gly Ala Leu Gln Lys Glu Leu Ile Arg
115 120 125
Phe Leu Asn Ala Gly Ile Phe Gly Asn Gly Thr Glu Ser Cys His Thr
130 135 140
Leu Pro His Ser Ala Thr Arg Ala Ala Met Leu Val Arg Ile Asn Thr
145 150 155 160
Leu Leu Gln Gly Tyr Ser Gly Ile Arg Phe Glu Ile Leu Glu Ala Ile
165 170 175
Thr Lys Leu Leu Asn His Asn Ile Thr Pro Cys Leu Pro Leu Arg Gly
180 185 190
Thr Val Thr Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr Ile Ala Gly
195 200 205
Leu Leu Thr Gly Arg Pro Asn Ser Lys Ala Val Gly Pro Ser Gly Glu
210 215 220
Val Val Asn Ala Glu Glu Ala Phe Lys Met Ala Gly Ile Glu Ser Gly
225 230 235 240
Phe Phe Glu Leu Gln Pro Lys Glu Gly Leu Ala Leu Val Asn Gly Thr
245 250 255
Ala Val Gly Ser Gly Leu Ala Ser Met Val Leu Phe Glu Thr Asn Val
260 265 270
Leu Ala Val Leu Ser Glu Val Leu Ser Ala Ile Phe Ala Glu Val Met
275 280 285
Gln Gly Lys Pro Glu Phe Thr Asp His Leu Thr His Lys Leu Lys His
290 295 300
His Pro Gly Gln Ile Glu Ala Ala Ala Ile Met Glu His Ile Leu Asp
305 310 315 320
Gly Ser Ser Tyr Val Lys Glu Ala Lys Lys Leu His Glu Met Asp Pro
325 330 335
Leu Gln Lys Pro Lys Gln Asp Arg Tyr Ala Leu Arg Thr Ser Pro Gln
340 345 350
Trp Leu Gly Pro His Ile Glu Val Ile Arg Ala Ser Thr Lys Ser Ile
355 360 365
Glu Arg Glu Ile Asn Ser Val Asn Asp Asn Pro Leu Ile Asp Val Ser
370 375 380
Arg Asn Lys Ala Leu His Gly Gly Asn Phe Gln Gly Thr Pro Ile Gly
385 390 395 400
Val Ser Met Asp Asn Thr Arg Leu Ala Ile Ala Ala Ile Gly Lys Leu
405 410 415
Met Phe Ala Gln Phe Ser Glu Leu Val Asn Asp Phe Tyr Asn Asn Gly
420 425 430
Leu Pro Ser Asn Leu Ser Gly Ser Arg Asn Pro Ser Leu Asp Tyr Gly
435 440 445
Phe Lys Gly Ala Glu Ile Ala Met Ala Ser Tyr Cys Ser Glu Leu Gln
450 455 460
Phe Leu Ala Asn Pro Val Thr Asn His Val Glu Ser Ala Glu Gln His
465 470 475 480
Asn Gln Asp Val Asn Ser Leu Gly Leu Ile Ser Ser Arg Lys Thr Ala
485 490 495
Glu Ala Val Asp Ile Leu Lys Leu Met Ser Thr Thr Tyr Leu Val Ala
500 505 510
Leu Cys Gln Ala Ile Asp Leu Arg His Leu Glu Glu Asn Leu Lys Ser
515 520 525
Thr Val Lys Lys Thr Val Ser His Val Ala Lys Lys Thr Leu Thr Thr
530 535 540
Gly Ala Asn Gly Glu Leu His Pro Ser Arg Phe Cys Glu Lys Asp Leu
545 550 555 560
Leu Lys Val Val Asp Arg Glu His Val Phe Ala Tyr Ile Asp Asp Pro
565 570 575
Cys Ser Ala Thr Tyr Pro Leu Met Gln Lys Val Arg Gln Val Leu Val
580 585 590
Glu His Ala Leu Asn Asn Gly Glu Asn Glu Lys Asn Gly Ser Thr Ser
595 600 605
Ile Phe Gln Lys Ile Gly Ala Phe Glu Glu Glu Leu Lys Ala Val Leu
610 615 620
Pro Lys Glu Val Glu Ser Ala Arg Asp Gly Val Glu Ser Gly Asn Pro
625 630 635 640
Ser Ile Pro Asn Arg Ile Lys Glu Cys Arg Ser Tyr Pro Leu Tyr Lys
645 650 655
Phe Val Arg Glu Glu Leu Gly Thr Gly Leu Leu Thr Gly Glu Lys Val
660 665 670
Arg Ser Pro Gly Glu Asp Phe Asp Lys Val Phe Thr Ala Met Cys Glu
675 680 685
Gly Lys Ile Ile Asp Pro Leu Leu Asp Cys Leu Ser Ala Trp Asn Gly
690 695 700
Ala Pro Leu Pro Ile Cys
705 710
<210> 113
<211> 713
<212> PRT
<213> 麻疯树()
<220>
<223> 苯丙氨酸氨裂合酶 (JcPAL)
<400> 113
Met Ala Thr Ile Ile Gly Asn Gly His Gln Asn Gly Ser Leu Glu Gly
1 5 10 15
Leu Cys Ile Thr Arg Asp Pro Leu Ser Trp Gly Val Ala Ala Glu Ser
20 25 30
Met Lys Gly Ser His Leu Asp Glu Val Lys Lys Met Val Ser Glu Tyr
35 40 45
Arg Lys Pro Leu Val Lys Leu Gly Gly Glu Thr Leu Thr Val Ala Gln
50 55 60
Val Ala Ala Ile Ala Ser His Asp Ala Gly Val Lys Val Glu Leu Ala
65 70 75 80
Glu Ser Ala Arg Ala Gly Val Lys Ala Ser Ser Asp Trp Val Met Asp
85 90 95
Ser Met Asn Lys Gly Thr Asp Ser Tyr Gly Val Thr Thr Gly Phe Gly
100 105 110
Ala Thr Ser His Arg Arg Thr Lys Gln Gly Ala Ala Leu Gln Arg Glu
115 120 125
Leu Ile Arg Phe Leu Asn Ala Gly Ile Phe Gly Asn Gly Thr Glu Thr
130 135 140
Cys His Thr Leu Pro His Ser Ala Thr Arg Ala Ala Met Leu Val Arg
145 150 155 160
Ile Asn Thr Leu Leu Gln Gly Tyr Ser Gly Ile Arg Phe Glu Ile Leu
165 170 175
Glu Ala Ile Thr Lys Leu Leu Asn His Asn Ile Thr Pro Cys Leu Pro
180 185 190
Leu Arg Gly Thr Ile Thr Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr
195 200 205
Ile Ala Gly Leu Leu Thr Gly Arg Pro Asn Ser Lys Ala Ile Gly Pro
210 215 220
Ser Gly Glu Ser Leu Asp Ala Val Glu Ala Phe Arg Leu Ala Asp Ile
225 230 235 240
Asp Ser Gly Phe Phe Glu Leu Gln Pro Lys Glu Gly Leu Ala Leu Val
245 250 255
Asn Gly Thr Ala Val Gly Ser Gly Leu Ala Ser Met Val Leu Phe Glu
260 265 270
Ala Asn Val Leu Ala Val Leu Ser Glu Ile Leu Ser Ala Ile Phe Ala
275 280 285
Glu Val Met Asn Gly Lys Pro Glu Phe Thr Asp His Leu Thr His Lys
290 295 300
Leu Lys His His Pro Gly Gln Ile Glu Ala Ala Ala Ile Met Glu His
305 310 315 320
Ile Leu Asp Gly Ser Ser Tyr Ile Lys Ala Ala Lys Gln Leu His Glu
325 330 335
Ile Asp Pro Leu Gln Lys Pro Lys Gln Asp Arg Tyr Ala Leu Arg Thr
340 345 350
Ser Pro Gln Trp Leu Gly Pro Gln Ile Glu Val Ile Arg Phe Ser Thr
355 360 365
Lys Ser Ile Glu Arg Glu Ile Asn Ser Val Asn Asp Asn Pro Leu Ile
370 375 380
Asp Val Ser Arg Asn Lys Ala Leu His Gly Gly Asn Phe Gln Gly Thr
385 390 395 400
Pro Ile Gly Val Ser Met Asp Asn Ala Arg Leu Ala Ile Ala Ser Ile
405 410 415
Gly Lys Leu Met Phe Ala Gln Phe Ser Glu Leu Val Asn Asp Phe Tyr
420 425 430
Asn Asn Gly Leu Pro Ser Asn Leu Ser Gly Gly Arg Asn Pro Ser Leu
435 440 445
Asp Tyr Gly Phe Lys Gly Ala Glu Ile Ala Met Ala Ser Tyr Cys Ser
450 455 460
Glu Leu Gln Phe Leu Ala Asn Pro Val Thr Ser His Val Gln Ser Ala
465 470 475 480
Glu Gln His Asn Gln Asp Val Asn Ser Leu Gly Leu Ile Ser Ser Arg
485 490 495
Lys Thr Gln Glu Ala Ile Asp Ile Leu Lys Leu Met Ser Ser Thr Phe
500 505 510
Leu Val Ala Leu Cys Gln Ala Ile Asp Leu Arg His Leu Glu Glu Asn
515 520 525
Leu Lys His Ala Val Lys Asn Thr Val Thr Gln Val Ala Lys Arg Val
530 535 540
Leu Thr Thr Gly Ala Asn Gly Glu Leu His Pro Ser Arg Phe Cys Gly
545 550 555 560
Lys Asp Leu Leu Lys Val Val Asp Arg Glu Gln Val Phe Ala Tyr Ile
565 570 575
Asp Asp Pro Cys Ser Ala Thr Tyr Pro Leu Met Gln Lys Leu Arg Gln
580 585 590
Val Leu Val Glu His Ala Leu Ala Asn Gly Glu Asn Glu Lys Asn Ala
595 600 605
Ser Thr Ser Val Phe Gln Lys Ile Gly Ala Phe Glu Glu Glu Leu Lys
610 615 620
Thr Leu Leu Pro Lys Glu Val Glu Ser Ala Arg Glu Ala Tyr Glu Ser
625 630 635 640
Gly Ser Ala Ala Ile Gly Asn Lys Ile Lys Glu Cys Arg Ser Tyr Pro
645 650 655
Leu Tyr Lys Phe Val Arg Glu Glu Leu Gly Ser Gly Leu Leu Thr Gly
660 665 670
Glu Lys Val Arg Ser Pro Gly Glu Glu Phe Asp Lys Val Phe Thr Ala
675 680 685
Met Cys Glu Gly Lys Ile Ile Asp Pro Met Met Glu Cys Leu Lys Glu
690 695 700
Trp Asn Gly Ala Pro Leu Pro Ile Cys
705 710
<210> 114
<211> 721
<212> PRT
<213> 一品红()
<220>
<223> 一品红苯丙氨酸氨裂合酶 (EpPAL)
<400> 114
Met Glu Ser Ile His Gln Asn Gly Phe Lys Asn Gly Ser Leu Asn Ser
1 5 10 15
Leu Cys Thr Asp Ser Glu Ser Ile Arg Ser His Asp Pro Leu Ser Trp
20 25 30
Gly Leu Ala Ala Glu Ser Met Lys Gly Ser His Leu Asp Glu Val Lys
35 40 45
Arg Met Val Ala Glu Phe Arg Lys Pro Val Val Lys Leu Gly Gly Glu
50 55 60
Thr Leu Thr Val Ala Gln Val Ala Ala Ile Ala Ala Ser Asn Gly Ser
65 70 75 80
Glu Asn Ile Arg Val Glu Leu Ala Glu Ser Ala Arg Ala Gly Val Lys
85 90 95
Ala Ser Ser Asp Trp Val Met Asp Ser Met Asn Asn Gly Thr Asp Ser
100 105 110
Tyr Gly Val Thr Thr Gly Phe Gly Ala Thr Ser His Arg Arg Thr Lys
115 120 125
Gln Gly Gly Ala Leu Gln Lys Glu Leu Ile Arg Phe Leu Asn Ala Gly
130 135 140
Ile Phe Gly Asn Gly Thr Glu Thr Ser His Ile Leu Pro His Ser Ala
145 150 155 160
Thr Lys Ala Ala Met Ile Val Arg Ile Asn Thr Leu Leu Gln Gly Tyr
165 170 175
Ser Gly Ile Arg Phe Glu Ile Leu Glu Ala Ile Thr Lys Leu Leu Asn
180 185 190
Thr Asn Ile Thr Pro Cys Leu Pro Leu Arg Gly Thr Ile Thr Ala Ser
195 200 205
Gly Asp Leu Val Pro Leu Ser Tyr Ile Ala Gly Leu Leu Thr Gly Arg
210 215 220
Ser Asn Ser Lys Ala Thr Gly Pro Asn Gly Glu Ser Leu Asp Ala Gln
225 230 235 240
Gln Ala Phe Ser Glu Ala Gly Ile Asp Ser Gly Leu Phe Glu Leu Gln
245 250 255
Pro Lys Glu Gly Leu Ala Leu Val Asn Gly Thr Ala Val Gly Ser Gly
260 265 270
Leu Ala Ser Met Val Leu Phe Asp Ala Asn Ile Leu Ala Val Leu Ser
275 280 285
Glu Val Leu Ser Ala Ile Phe Ala Glu Val Met Asn Gly Lys Pro Glu
290 295 300
Phe Thr Asp His Leu Thr His Lys Leu Lys His His Pro Gly Gln Ile
305 310 315 320
Glu Ala Ala Ala Ile Met Glu His Ile Leu Asp Gly Ser Gly Tyr Val
325 330 335
Lys Ala Ala Lys Lys Val His Glu Met Asp Pro Leu Gln Lys Pro Lys
340 345 350
Gln Asp Arg Tyr Ala Leu Arg Thr Ser Pro Gln Trp Leu Gly Pro Gln
355 360 365
Ile Glu Val Ile Arg Phe Ser Thr Lys Ser Ile Glu Arg Glu Ile Asn
370 375 380
Ser Val Asn Asp Asn Pro Leu Ile Asp Val Ser Arg Asn Lys Ala Leu
385 390 395 400
His Gly Gly Asn Phe Gln Gly Thr Pro Ile Gly Val Ser Met Asp Asn
405 410 415
Ala Arg Leu Ala Ile Ala Ser Ile Gly Lys Leu Met Phe Ala Gln Phe
420 425 430
Ser Glu Leu Val Asn Asp Phe Tyr Asn Asn Gly Leu Pro Ser Asn Leu
435 440 445
Thr Ala Gly Arg Asn Pro Ser Leu Asp Tyr Gly Phe Lys Gly Ala Glu
450 455 460
Ile Ala Met Ala Ser Tyr Cys Ser Glu Leu Gln Tyr Leu Ala Asn Pro
465 470 475 480
Val Thr Ser His Val Gln Ser Ala Glu Gln His Asn Gln Asp Val Asn
485 490 495
Ser Leu Gly Leu Ile Ser Ser Arg Lys Thr Gln Glu Ser Val Asp Ile
500 505 510
Leu Lys Leu Met Ser Thr Thr Phe Leu Val Ala Leu Cys Gln Ala Ile
515 520 525
Asp Leu Arg His Met Glu Glu Asn Leu Arg Gln Thr Val Lys Asn Thr
530 535 540
Val Ser Gln Val Ala Lys Arg Val Leu Thr Thr Gly Ala Asn Gly Glu
545 550 555 560
Leu His Pro Ser Arg Phe Cys Glu Lys Glu Leu Leu Lys Val Val Asp
565 570 575
Arg Glu Tyr Val Phe Ala Tyr Ala Asp Asp Pro Cys Ser Ser Thr Tyr
580 585 590
Pro Leu Met Gln Lys Leu Arg Gln Val Ile Val Asp His Ala Leu Leu
595 600 605
Asn Gly Asp Asn Glu Lys Asn Ala Asn Thr Ser Ile Phe Gln Lys Ile
610 615 620
Ser Ala Phe Glu Ala Glu Leu Lys Thr Leu Leu Pro Lys Glu Val Glu
625 630 635 640
Ala Ala Arg Ala Ala Tyr Glu Ser Gly Asn Ala Pro Ile Pro Asn Arg
645 650 655
Ile Met Glu Cys Arg Ser Tyr Pro Leu Tyr Lys Phe Val Arg Glu Glu
660 665 670
Gln Gly Thr Gly Ile Leu Thr Gly Asp Lys Val Arg Ser Pro Gly Glu
675 680 685
Glu Phe Asp Lys Val Phe Thr Ala Met Cys Gln Gly Lys Ile Ile Asp
690 695 700
Pro Leu Met Asp Cys Leu Lys Glu Trp Asn Gly Ala Pro Leu Pro Ile
705 710 715 720
Cys
<210> 115
<211> 725
<212> PRT
<213> 红车轴草()
<220>
<223> 三叶草苯丙氨酸氨裂合酶 (TpPAL)
<400> 115
Met Glu Ala Val Ala Ala Ala Ile Thr Lys Asn Asn Ile Asn Asp Tyr
1 5 10 15
Asp Ser Phe Cys Leu Thr His Ala Asn Ala Asn Asn Met Lys Val Asn
20 25 30
Gly Ala Asp Pro Leu Asn Trp Gly Val Ala Ala Glu Ala Met Lys Gly
35 40 45
Ser His Leu Asp Glu Val Lys Arg Met Val Glu Glu Tyr Arg Lys Pro
50 55 60
Val Val Arg Leu Gly Gly Glu Thr Leu Thr Ile Ser Gln Val Ala Ala
65 70 75 80
Ile Ala Ala His Asp Gly Ala Thr Val Glu Leu Ser Glu Ser Ala Arg
85 90 95
Ala Gly Val Lys Ala Ser Ser Asp Trp Ile Met Glu Ser Met Asn Lys
100 105 110
Gly Thr Asp Ser Tyr Gly Val Thr Thr Gly Phe Gly Ala Thr Ser His
115 120 125
Arg Arg Thr Lys Gln Gly Gly Ala Leu Gln Lys Glu Leu Ile Arg Phe
130 135 140
Leu Asn Ala Gly Ile Phe Gly Asn Gly Thr Glu Ser Asn His Ile Leu
145 150 155 160
Pro His Thr Ala Thr Arg Ala Ala Met Leu Val Arg Ile Asn Thr Leu
165 170 175
Leu Gln Gly Tyr Ser Gly Ile Arg Phe Glu Ile Leu Glu Ala Ile Thr
180 185 190
Lys Leu Leu Asn Asn Asn Ile Thr Pro Cys Leu Pro Leu Arg Gly Thr
195 200 205
Ile Thr Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr Ile Ala Gly Leu
210 215 220
Leu Thr Gly Arg Pro Asn Ser Lys Ala His Gly Pro Ser Gly Glu Ile
225 230 235 240
Leu Asn Ala Lys Glu Ala Phe Gln Leu Ala Gly Ile Asn Ala Asp Phe
245 250 255
Phe Glu Leu Gln Pro Lys Glu Gly Leu Ala Leu Val Asn Gly Thr Ala
260 265 270
Val Gly Ser Gly Leu Ala Ser Ile Val Leu Phe Glu Ala Asn Ile Leu
275 280 285
Ala Val Leu Ser Glu Val Leu Ser Ala Ile Phe Ala Glu Val Met Gln
290 295 300
Gly Lys Pro Glu Phe Thr Asp His Leu Thr His Lys Leu Lys His His
305 310 315 320
Pro Gly Gln Ile Glu Ala Ala Ala Ile Met Glu His Ile Leu Asp Gly
325 330 335
Ser Ala Tyr Val Lys Ala Ala Lys Lys Leu His Glu Ile Asp Pro Leu
340 345 350
Gln Lys Pro Lys Gln Asp Arg Tyr Ala Leu Arg Thr Ser Pro Gln Trp
355 360 365
Leu Gly Pro Leu Ile Glu Val Ile Arg Phe Ser Thr Lys Ser Ile Glu
370 375 380
Arg Glu Ile Asn Ser Val Asn Asp Asn Pro Leu Ile Asp Val Ser Arg
385 390 395 400
Asn Lys Ala Leu His Gly Gly Asn Phe Gln Gly Thr Pro Ile Gly Val
405 410 415
Ser Met Asp Asn Thr Arg Leu Ala Leu Ala Ser Ile Gly Lys Leu Met
420 425 430
Phe Ala Gln Phe Ser Glu Leu Val Asn Asp Phe Tyr Asn Asn Gly Leu
435 440 445
Pro Ser Asn Leu Ser Ala Ser Arg Asn Pro Ser Leu Asp Tyr Gly Phe
450 455 460
Lys Gly Ser Glu Ile Ala Met Ala Ser Tyr Cys Ser Glu Leu Gln Tyr
465 470 475 480
Leu Ala Asn Pro Val Thr Thr His Val Gln Ser Ala Glu Gln His Asn
485 490 495
Gln Asp Val Asn Ser Leu Gly Leu Ile Ser Ser Arg Lys Thr Lys Glu
500 505 510
Ala Ile Glu Ile Leu Gln Leu Met Ser Ser Thr Phe Leu Ile Ala Leu
515 520 525
Cys Gln Ala Ile Asp Leu Arg His Leu Glu Glu Asn Leu Lys Asn Ser
530 535 540
Val Lys Asn Thr Val Ser Gln Val Ala Lys Lys Thr Leu Thr Val Gly
545 550 555 560
Val Asn Gly Glu Leu His Pro Ser Arg Phe Cys Glu Lys Asp Leu Leu
565 570 575
Lys Val Val Asp Arg Glu His Val Phe Ser Tyr Ile Asp Asp Pro Cys
580 585 590
Ser Ala Thr Tyr Pro Leu Ser Gln Lys Leu Arg Gln Val Leu Val Glu
595 600 605
His Ala Leu Val Asn Gly Glu Ser Glu Lys Asn Ser Asn Thr Ser Ile
610 615 620
Phe Gln Lys Ile Ala Thr Phe Glu Glu Glu Leu Lys Thr Leu Leu Pro
625 630 635 640
Lys Glu Val Glu Ser Ala Arg Thr Ala Tyr Glu Ser Gly Asn Ser Thr
645 650 655
Ile Ala Asn Lys Ile Asn Gly Cys Arg Ser Tyr Pro Leu Tyr Lys Phe
660 665 670
Val Arg Glu Glu Leu Gly Thr Ser Leu Leu Thr Gly Glu Arg Val Ile
675 680 685
Ser Pro Gly Glu Glu Cys Asp Lys Leu Phe Thr Ala Met Cys Gln Gly
690 695 700
Lys Ile Ile Asp Pro Leu Leu Glu Cys Leu Gly Glu Trp Asn Gly Ser
705 710 715 720
Pro Leu Pro Ile Cys
725
<210> 116
<211> 637
<212> PRT
<213> 日本百脉根()
<220>
<223> 苯丙氨酸氨裂合酶 (LjPAL5)
<400> 116
Met Ala Pro Thr Thr Asn Ser Asn His Glu Ser Leu Asn Ser Ser Ile
1 5 10 15
Phe Cys Thr Ala Ala Lys Ala Gly Ser Asp Pro Leu Ser Trp Gly Val
20 25 30
Ala Ala Asp Ser Met Lys Gly Ser His Leu Asp Glu Val Lys Arg Met
35 40 45
Val Ala Glu Tyr Arg Lys Pro Val Val Lys Leu Gly Gly Glu Thr Leu
50 55 60
Thr Ile Ala Gln Val Ala Ala Ile Ala Ala Asn Asp Gln Gly Val Ser
65 70 75 80
Val Glu Leu Cys Glu Ser Ala Arg Ala Gly Val Lys Ala Ser Ser Asp
85 90 95
Trp Val Met Asn Ser Met Asn Asn Gly Thr Asp Ser Tyr Gly Val Thr
100 105 110
Thr Gly Phe Gly Ala Thr Ser His Arg Arg Thr Lys Asn Gly Asn Ala
115 120 125
Leu Gln Leu Glu Leu Ile Arg Phe Leu Asn Ala Gly Ile Phe Gly Asn
130 135 140
Gly Thr Glu Thr Ser His Thr Leu Pro Gln Pro Ala Thr Arg Ala Ala
145 150 155 160
Met Leu Val Arg Ile Asn Thr Leu Leu Gln Gly Tyr Ser Gly Ile Arg
165 170 175
Phe Glu Ile Leu Glu Ala Ile Thr Lys Leu Ile Asn Asn Asn Ile Thr
180 185 190
Pro Cys Leu Pro Leu Arg Gly Thr Val Thr Ala Ser Gly Asp Leu Val
195 200 205
Pro Leu Ser Tyr Ile Ala Gly Leu Leu Thr Gly Arg Gln Asn Ser Lys
210 215 220
Ala Val Gly Pro Ser Gly Glu Val Val Asn Ala Lys Glu Ala Phe Gln
225 230 235 240
Leu Ala Gly Ile Asp Thr Gly Phe Phe Glu Leu Gln Pro Lys Glu Gly
245 250 255
Leu Ala Leu Val Asn Gly Thr Ala Val Gly Ser Gly Leu Ala Ser Ile
260 265 270
Val Leu Phe Asp Ala Asn Val Leu Ala Ile Leu Ala Glu Val Leu Ser
275 280 285
Ala Ile Phe Ala Glu Val Met Glu Arg Glu Ile Asn Ser Val Asn Asp
290 295 300
Asn Pro Leu Ile Asp Val Ser Arg Asn Lys Ala Leu His Gly Gly Asn
305 310 315 320
Phe Gln Gly Thr Pro Ile Gly Val Ser Met Asp Asn Thr Arg Leu Ala
325 330 335
Leu Ala Ala Ile Gly Lys Leu Met Phe Ala Gln Phe Thr Glu Leu Val
340 345 350
Asp Asp His Tyr Asn Asn Gly Leu Pro Ser Asn Leu Thr Ala Ser Arg
355 360 365
Asn Pro Ser Leu Asp Tyr Gly Leu Lys Gly Ala Glu Ile Ala Met Ala
370 375 380
Ser Tyr Cys Ser Glu Leu Gln Tyr Leu Ala Asn Pro Val Thr Thr His
385 390 395 400
Val Gln Ser Ala Glu Gln His Asn Gln Asp Val Asn Ser Leu Gly Leu
405 410 415
Ile Ser Ser Arg Lys Thr Asn Glu Ala Ile Glu Ile Leu Lys Leu Met
420 425 430
Ser Ser Thr Phe Leu Ile Ala Leu Cys Gln Ala Ile Asp Leu Arg His
435 440 445
Leu Glu Glu Asn Leu Lys His Ser Val Lys Ser Thr Val Ser Gln Val
450 455 460
Ala Lys Arg Thr Leu Thr Thr Ser Val Asn Gly Glu Leu His Pro Ser
465 470 475 480
Arg Phe Cys Glu Lys Asp Leu Leu Lys Val Val Asp Arg Glu Thr Leu
485 490 495
Phe Ala Tyr Ile Asp Asp Pro Cys Ser Ala Thr Tyr Pro Leu Met Gln
500 505 510
Lys Leu Arg Gln Val Leu Val Asp His Ala Leu Val Asn Gly Glu Asn
515 520 525
Glu Lys Asn Ser Lys Thr Ser Ile Phe Gln Lys Ile Ala Thr Phe Glu
530 535 540
Asp Glu Leu Lys Ser Leu Leu Pro Lys Glu Val Glu Ser Ala Arg Ala
545 550 555 560
Ala Tyr Glu Ser Gly Asn Pro Thr Ile Pro Asn Lys Ile Asn Glu Cys
565 570 575
Arg Ser Tyr Pro Leu Tyr Lys Phe Val Arg Glu Glu Leu Gly Thr Glu
580 585 590
Leu Leu Thr Gly Glu Lys Thr Arg Ser Pro Gly Glu Glu Cys Asp Lys
595 600 605
Leu Phe Thr Ala Ile Cys Gln Gly Lys Ile Ile Asp Pro Leu Leu Glu
610 615 620
Cys Leu Gly Glu Trp Asn Gly Ala Pro Leu Pro Ile Cys
625 630 635
<210> 117
<211> 681
<212> PRT
<213> 江南卷柏()
<220>
<223> 卷柏苯丙氨酸氨裂合酶 (SmPAL)
<400> 117
Met Ser Gly Ser His Leu Glu Glu Val Arg Glu Met Val His Thr Val
1 5 10 15
Tyr Gly Ala Ala Lys Pro Ser Phe Pro Ile Glu Gly Thr Thr Leu Thr
20 25 30
Ile Ala Gln Val Ala Ala Val Ala Lys Arg Gly Ala Ala Ala Gln Val
35 40 45
Arg Leu Asp Ser Ala Ala Ala Lys Lys Arg Val Asp Glu Ser Ser Asn
50 55 60
Trp Val Leu Asp Asn Ala Met Lys Gly Thr Asp Thr Tyr Gly Val Thr
65 70 75 80
Thr Gly Phe Gly Ala Thr Ser His Arg Arg Thr Asn Gln Gly Val Glu
85 90 95
Leu Gln Arg Glu Leu Ile Arg Phe Leu Asn Ala Gly Ile Phe Ser Glu
100 105 110
Asp Asp Ser Thr Asn Val Leu Pro Leu Ala Phe Ala Arg Ala Ala Met
115 120 125
Leu Val Arg Thr Asn Thr Leu Leu Gln Gly Tyr Ser Gly Ile Arg Trp
130 135 140
Glu Ile Leu Ser Ala Met Glu Lys Leu Val Asn Ser Gly Ile Val Ala
145 150 155 160
Arg Ile Pro Leu Arg Gly Thr Ile Thr Ala Ser Gly Asp Leu Val Pro
165 170 175
Leu Ser Tyr Ile Ala Gly Leu Leu Thr Gly Arg Ser Asn Ala Arg Ala
180 185 190
Val Leu Pro Asp Gly Lys Val Val Thr Ser Ala Glu Ala Leu Lys Leu
195 200 205
Val Gly Val Glu Gln Pro Phe Glu Leu Gln Pro Lys Glu Gly Leu Ala
210 215 220
Ile Val Asn Gly Thr Ala Val Gly Ala Ala Val Ala Ser Ile Ala Cys
225 230 235 240
Phe Asp Ala Asn Val Leu Ala Leu Leu Ala Glu Ile Leu Ser Ala Met
245 250 255
Phe Cys Glu Ala Met Gln Gly Lys Pro Glu Phe Thr Asp Pro Leu Thr
260 265 270
His Lys Leu Lys His His Pro Gly Gln Ile Glu Ala Ala Ala Ile Met
275 280 285
Glu His Val Leu Ala Gly Ser Ser Tyr Met Lys Ala Ala Ala Lys Leu
290 295 300
His Glu Thr Asp Pro Leu Lys Lys Pro Lys Gln Asp Arg Tyr Ala Leu
305 310 315 320
Arg Thr Ser Pro Gln Trp Leu Gly Pro Gln Ile Glu Val Ile Arg His
325 330 335
Ala Thr His Ser Ile Gln Arg Glu Ile Asn Ser Val Asn Asp Asn Pro
340 345 350
Ile Ile Asp Val Ala Arg Asp Lys Ala Leu His Gly Gly Asn Phe Gln
355 360 365
Gly Thr Pro Ile Gly Val Ser Met Asp Asn Leu Arg Leu Ala Val Ala
370 375 380
Ala Ile Gly Lys Leu Met Phe Ala Gln Phe Ser Glu Leu Val Asn Asp
385 390 395 400
Phe Tyr Asn Asn Gly Leu Pro Ser Asn Leu Ser Gly Gly Ser Asn Pro
405 410 415
Ser Leu Asp Tyr Gly Phe Lys Gly Ala Glu Ile Ala Met Ala Ser Tyr
420 425 430
Thr Ser Glu Leu Gln Tyr Leu Ala Asn Pro Val Thr Thr His Val Gln
435 440 445
Ser Ala Glu Gln His Asn Gln Asp Val Asn Ser Leu Gly Leu Val Ser
450 455 460
Ala Arg Lys Thr Val Glu Ala Leu Asp Ile Leu Lys Leu Met Ser Ser
465 470 475 480
Thr Tyr Leu Val Ala Leu Cys Gln Ala Ile Asp Leu Arg His Leu Glu
485 490 495
Glu Asn Leu Gln Ala Thr Val Lys Gln Thr Val Ser His Ala Val Lys
500 505 510
Lys Thr Leu Thr Thr Gly Ala Ala Gly Ala Leu Leu Pro Ser Arg Phe
515 520 525
Cys Glu Lys Glu Leu Leu Ser Val Val Asp Asn Gln His Val Phe Thr
530 535 540
Tyr Ile Asp Asp Pro Ala Ser Ala Gly Tyr Pro Leu Met Gln Lys Leu
545 550 555 560
Arg Gln Val Leu Val Glu His Ala Leu Lys Asn Ile Gly Asp Glu Lys
565 570 575
Glu Thr Ser Ser Ser Val Leu His Lys Ile Gly Leu Phe Glu Glu Glu
580 585 590
Leu Lys Ala Ala Leu Ser Val Glu Val Pro Ala Ala Arg Glu Ala Tyr
595 600 605
Glu Ser Gly Asn Ala Val Leu Pro Asn Arg Ile Phe Asp Cys Ala Ser
610 615 620
Ala Pro Leu Tyr Glu Phe Val Arg Lys Gly Ala Gly Thr Ala Leu Leu
625 630 635 640
Met Gly Thr Lys Ser Gly Thr Pro Gly Glu Asp Phe Thr Lys Val Tyr
645 650 655
Asp Ala Ile Cys Gln Gly Lys Leu Val Thr Pro Leu Leu Lys Cys Leu
660 665 670
Asp Gly Trp Ser Gly Thr Pro Ser Phe
675 680
<210> 118
<211> 505
<212> PRT
<213> 人工序列()
<220>
<223> 合成的肉桂酸4-羟化酶 (C4H) 共有 序列（大多数）
<220>
<221> 变体
<222> (22)..(.22)
<223> Xaa = Val, Leu, Ala, Thr,或Ile
<220>
<221> 变体
<222> (152)..(.152)
<223> Xaa = Phe, Glu, Ala, Ser, Asp或Gln
<220>
<221> 变体
<222> (334)..(.334)
<223> Xaa = Asn, His, Glu, Gln或Asp
<220>
<221> 变体
<222> (426)..(.426)
<223> Xaa = Ser, Ala或Lys
<220>
<221> 变体
<222> (461)..(.461)
<223> Xaa = Ile或Leu
<220>
<221> UNSURE
<222> (22)..(22)
<223> The 'Xaa' at location 22 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (152)..(152)
<223> The 'Xaa' at location 152 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (334)..(334)
<223> The 'Xaa' at location 334 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (426)..(426)
<223> The 'Xaa' at location 426 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (461)..(461)
<223> The 'Xaa' at location 461 stands for Gln, Arg, Pro, or Leu.
<400> 118
Met Asp Leu Leu Leu Leu Glu Lys Thr Leu Leu Gly Leu Phe Ala Ala
1 5 10 15
Val Val Val Ala Ile Xaa Val Ser Lys Leu Arg Gly Lys Arg Phe Lys
20 25 30
Leu Pro Pro Gly Pro Ile Pro Val Pro Ile Phe Gly Asn Trp Leu Gln
35 40 45
Val Gly Asp Asp Leu Asn His Arg Asn Leu Thr Asp Leu Ala Lys Lys
50 55 60
Phe Gly Asp Ile Phe Leu Leu Arg Met Gly Gln Arg Asn Leu Val Val
65 70 75 80
Val Ser Ser Pro Glu Leu Ala Lys Glu Val Leu His Thr Gln Gly Val
85 90 95
Glu Phe Gly Ser Arg Thr Arg Asn Val Val Phe Asp Ile Phe Thr Gly
100 105 110
Lys Gly Gln Asp Met Val Phe Thr Val Tyr Gly Glu His Trp Arg Lys
115 120 125
Met Arg Arg Ile Met Thr Val Pro Phe Phe Thr Asn Lys Val Val Gln
130 135 140
Gln Tyr Arg Tyr Gly Trp Glu Xaa Glu Ala Ala Ser Val Val Glu Asp
145 150 155 160
Val Lys Lys Asn Pro Glu Ala Ala Thr Asn Gly Ile Val Leu Arg Arg
165 170 175
Arg Leu Gln Leu Met Met Tyr Asn Asn Met Tyr Arg Ile Met Phe Asp
180 185 190
Arg Arg Phe Glu Ser Glu Asp Asp Pro Leu Phe Val Lys Leu Lys Ala
195 200 205
Leu Asn Gly Glu Arg Ser Arg Leu Ala Gln Ser Phe Glu Tyr Asn Tyr
210 215 220
Gly Asp Phe Ile Pro Ile Leu Arg Pro Phe Leu Arg Gly Tyr Leu Lys
225 230 235 240
Ile Cys Lys Glu Val Lys Glu Arg Arg Leu Gln Leu Phe Lys Asp Tyr
245 250 255
Phe Val Asp Glu Arg Lys Lys Leu Ala Ser Thr Lys Ser Thr Asp Ser
260 265 270
Asn Gly Leu Lys Cys Ala Ile Asp His Ile Leu Glu Ala Gln Gln Lys
275 280 285
Gly Glu Ile Asn Glu Asp Asn Val Leu Tyr Ile Val Glu Asn Ile Asn
290 295 300
Val Ala Ala Ile Glu Thr Thr Leu Trp Ser Ile Glu Trp Gly Ile Ala
305 310 315 320
Glu Leu Val Asn His Pro Glu Ile Gln Lys Lys Leu Arg Xaa Glu Leu
325 330 335
Asp Thr Val Leu Gly Pro Gly Val Gln Val Thr Glu Pro Asp Leu His
340 345 350
Lys Leu Pro Tyr Leu Gln Ala Val Ile Lys Glu Thr Leu Arg Leu Arg
355 360 365
Met Ala Ile Pro Leu Leu Val Pro His Met Asn Leu His Asp Ala Lys
370 375 380
Leu Ala Gly Tyr Asp Ile Pro Ala Glu Ser Lys Ile Leu Val Asn Ala
385 390 395 400
Trp Trp Leu Ala Asn Asn Pro Ala His Trp Lys Lys Pro Glu Glu Phe
405 410 415
Arg Pro Glu Arg Phe Leu Glu Glu Glu Xaa His Val Glu Ala Asn Gly
420 425 430
Asn Asp Phe Arg Tyr Leu Pro Phe Gly Val Gly Arg Arg Ser Cys Pro
435 440 445
Gly Ile Ile Leu Ala Leu Pro Ile Leu Gly Ile Thr Xaa Gly Arg Leu
450 455 460
Val Gln Asn Phe Glu Leu Leu Pro Pro Pro Gly Gln Ser Lys Ile Asp
465 470 475 480
Thr Thr Glu Lys Gly Gly Gln Phe Ser Leu His Ile Leu Lys His Ser
485 490 495
Thr Ile Val Ala Lys Pro Arg Ser Phe
500 505
<210> 119
<211> 505
<212> PRT
<213> 火炬松()
<220>
<223> 松树肉桂酸4-羟化酶 (PtC4H)
<400> 119
Met Asp Leu Leu Leu Leu Glu Lys Thr Leu Leu Gly Ser Phe Val Ala
1 5 10 15
Ile Leu Val Ala Ile Leu Val Ser Lys Leu Arg Gly Lys Arg Phe Lys
20 25 30
Leu Pro Pro Gly Pro Ile Pro Val Pro Val Phe Gly Asn Trp Leu Gln
35 40 45
Val Gly Asp Asp Leu Asn His Arg Asn Leu Thr Asp Leu Ala Lys Lys
50 55 60
Phe Gly Asp Ile Phe Leu Leu Arg Met Gly Gln Arg Asn Leu Val Val
65 70 75 80
Val Ser Ser Pro Asp Leu Ser Lys Glu Val Leu His Thr Gln Gly Val
85 90 95
Glu Phe Gly Ser Arg Thr Arg Asn Val Val Phe Asp Ile Phe Thr Gly
100 105 110
Lys Gly Gln Asp Met Val Phe Thr Val Tyr Gly Glu His Trp Arg Lys
115 120 125
Met Arg Arg Ile Met Thr Val Pro Phe Phe Thr Asn Lys Val Val Gln
130 135 140
Gln Tyr Arg Tyr Gly Trp Glu Glu Glu Ala Ala Gln Val Val Glu Asp
145 150 155 160
Val Lys Lys Asn Pro Glu Ala Ala Thr Asn Gly Ile Val Leu Arg Arg
165 170 175
Arg Leu Gln Leu Met Met Tyr Asn Asn Met Tyr Arg Ile Met Phe Asp
180 185 190
Arg Arg Phe Glu Ser Glu Asp Asp Pro Leu Phe Asn Lys Leu Lys Ala
195 200 205
Leu Asn Gly Glu Arg Ser Arg Leu Ala Gln Ser Phe Asp Tyr Asn Tyr
210 215 220
Gly Asp Phe Ile Pro Ile Leu Arg Pro Phe Leu Arg Gly Tyr Leu Lys
225 230 235 240
Ile Cys Gln Glu Val Lys Glu Arg Arg Leu Gln Leu Phe Lys Asp Tyr
245 250 255
Phe Val Asp Glu Arg Lys Lys Leu Ala Ser Thr Lys Asn Met Asn Asn
260 265 270
Glu Gly Leu Lys Cys Ala Ile Asp His Ile Leu Asp Asp Gln Lys Lys
275 280 285
Gly Glu Ile Asn Glu Asp Asn Val Leu Tyr Ile Val Glu Asn Ile Asn
290 295 300
Val Ala Ala Ile Glu Thr Thr Leu Trp Ser Ile Glu Trp Gly Ile Ala
305 310 315 320
Glu Leu Val Asn His Pro Glu Ile Gln Lys Lys Leu Arg His Glu Leu
325 330 335
Asp Thr Leu Leu Gly Pro Gly His Gln Ile Thr Glu Pro Asp Thr Tyr
340 345 350
Lys Leu Pro Tyr Leu Asn Ala Val Ile Lys Glu Thr Leu Arg Leu Arg
355 360 365
Met Ala Ile Pro Leu Leu Val Pro His Met Asn Leu His Asp Ala Lys
370 375 380
Leu Gly Gly Phe Asp Ile Pro Ala Glu Ser Lys Ile Leu Val Asn Ala
385 390 395 400
Trp Trp Leu Ala Asn Asn Pro Ala His Trp Lys Asn Pro Glu Glu Phe
405 410 415
Arg Pro Glu Arg Phe Leu Glu Glu Glu Ala Lys Val Glu Ala Asn Gly
420 425 430
Asn Asp Phe Arg Tyr Leu Pro Phe Gly Val Gly Arg Arg Ser Cys Pro
435 440 445
Gly Ile Ile Leu Ala Leu Pro Ile Leu Gly Ile Thr Leu Gly Arg Leu
450 455 460
Val Gln Asn Phe Glu Leu Leu Pro Pro Pro Gly Gln Ser Lys Ile Asp
465 470 475 480
Thr Ala Glu Lys Gly Gly Gln Phe Ser Leu His Ile Leu Lys His Ser
485 490 495
Thr Ile Val Ala Lys Pro Arg Ser Phe
500 505
<210> 120
<211> 500
<212> PRT
<213> 水稻()
<220>
<223> 稻肉桂酸4-羟化酶 (OsC4H)
<400> 120
Met Asp Ala Leu Leu Val Glu Lys Val Leu Leu Gly Leu Phe Val Ala
1 5 10 15
Ala Val Leu Ala Leu Val Val Ala Lys Leu Thr Gly Lys Arg Leu Arg
20 25 30
Leu Pro Pro Gly Pro Ala Gly Ala Pro Ile Val Gly Asn Trp Leu Gln
35 40 45
Val Gly Asp Asp Leu Asn His Arg Asn Leu Met Ala Leu Ala Arg Arg
50 55 60
Phe Gly Asp Ile Leu Leu Leu Arg Met Gly Val Arg Asn Leu Val Val
65 70 75 80
Val Ser Ser Pro Asp Leu Ala Lys Glu Val Leu His Thr Gln Gly Val
85 90 95
Glu Phe Gly Ser Arg Thr Arg Asn Val Val Phe Asp Ile Phe Thr Gly
100 105 110
Lys Gly Gln Asp Met Val Phe Thr Val Tyr Gly Asp His Trp Arg Lys
115 120 125
Met Arg Arg Ile Met Thr Val Pro Phe Phe Thr Asn Lys Val Val Ala
130 135 140
Gln Asn Arg Ala Gly Trp Glu Glu Glu Ala Arg Leu Val Val Glu Asp
145 150 155 160
Val Arg Arg Asp Pro Ala Ala Ala Thr Ser Gly Val Val Ile Arg Arg
165 170 175
Arg Leu Gln Leu Met Met Tyr Asn Asp Met Phe Arg Ile Met Phe Asp
180 185 190
Arg Arg Phe Asp Ser Val Asp Asp Pro Leu Phe Asn Lys Leu Lys Ala
195 200 205
Phe Asn Ala Glu Arg Ser Arg Leu Ser Gln Ser Phe Glu Tyr Asn Tyr
210 215 220
Gly Asp Phe Ile Pro Val Leu Arg Pro Phe Leu Arg Arg Tyr Leu Ala
225 230 235 240
Arg Cys His Gln Leu Lys Ser Gln Arg Met Lys Leu Phe Glu Asp His
245 250 255
Phe Val Gln Glu Arg Lys Arg Val Met Glu Gln Thr Gly Glu Ile Arg
260 265 270
Cys Ala Met Asp His Ile Leu Glu Ala Glu Arg Lys Gly Glu Ile Asn
275 280 285
His Asp Asn Val Leu Tyr Ile Val Glu Asn Ile Asn Val Ala Ala Ile
290 295 300
Glu Thr Thr Leu Trp Ser Ile Glu Trp Gly Ile Ala Glu Leu Val Asn
305 310 315 320
His Pro Ser Ile Gln Ser Lys Val Arg Glu Glu Met Ala Ser Val Leu
325 330 335
Gly Gly Ala Ala Val Thr Glu Pro Asp Leu Glu Arg Leu Pro Tyr Leu
340 345 350
Gln Ala Val Val Lys Glu Thr Leu Arg Leu Arg Met Ala Ile Pro Leu
355 360 365
Leu Val Pro His Met Asn Leu Ala Asp Gly Lys Leu Ala Gly Tyr Asp
370 375 380
Ile Pro Ala Glu Ser Lys Ile Leu Val Asn Ala Trp Phe Leu Ala Asn
385 390 395 400
Asp Pro Lys Arg Trp Val Arg Pro Asp Glu Phe Arg Pro Glu Arg Phe
405 410 415
Leu Glu Glu Glu Lys Ala Val Glu Ala His Gly Asn Asp Phe Arg Phe
420 425 430
Val Pro Phe Gly Val Gly Arg Arg Ser Cys Pro Gly Ile Ile Leu Ala
435 440 445
Leu Pro Ile Ile Gly Ile Thr Leu Gly Arg Leu Val Gln Ser Phe Asp
450 455 460
Leu Leu Pro Pro Pro Gly Met Asp Lys Val Asp Thr Thr Glu Lys Pro
465 470 475 480
Gly Gln Phe Ser Asn Gln Ile Leu Lys His Ala Thr Val Val Cys Lys
485 490 495
Pro Ile Asp Ala
500
<210> 121
<211> 501
<212> PRT
<213> 玉米()
<220>
<223> 玉米肉桂酸4-羟化酶 (ZmC4H)
<400> 121
Met Asp Leu Ala Leu Leu Glu Lys Ala Leu Leu Gly Leu Phe Ala Ala
1 5 10 15
Ala Val Val Ala Ile Ala Val Ala Lys Leu Ser Gly Lys Arg Tyr Arg
20 25 30
Leu Pro Pro Gly Pro Pro Gly Ala Pro Val Val Gly Asn Trp Leu Gln
35 40 45
Val Gly Asp Asp Leu Asn His Arg Asn Leu Met Ala Met Ala Lys Arg
50 55 60
Phe Gly Asp Ile Phe Leu Leu Arg Met Gly Val Arg Asn Leu Val Val
65 70 75 80
Val Ser Thr Pro Glu Leu Ala Lys Glu Val Leu His Thr Gln Gly Val
85 90 95
Glu Phe Gly Ser Arg Thr Arg Asn Val Val Phe Asp Ile Phe Thr Gly
100 105 110
Lys Gly Gln Asp Met Val Phe Thr Val Tyr Gly Asp His Trp Arg Lys
115 120 125
Met Arg Arg Ile Met Thr Val Pro Phe Phe Thr Asn Lys Val Val Ala
130 135 140
Gln Asn Arg Ala Gly Trp Glu Glu Glu Ala Arg Leu Val Val Glu Asp
145 150 155 160
Val Arg Lys Asp Pro Glu Ala Ala Ala Gly Gly Val Val Leu Arg Arg
165 170 175
Arg Leu Gln Leu Met Met Tyr Asn Asp Met Phe Arg Ile Met Phe Asp
180 185 190
Arg Arg Phe Asp Ser Glu His Asp Pro Leu Phe Asn Lys Leu Lys Ala
195 200 205
Leu Asn Ala Glu Arg Ser Arg Leu Ser Gln Ser Phe Glu Tyr Asn Tyr
210 215 220
Gly Asp Phe Ile Pro Val Leu Arg Pro Phe Leu Arg Gly Tyr Leu Asn
225 230 235 240
Arg Cys His Asp Leu Lys Thr Arg Arg Met Lys Phe Phe Glu Asp Asn
245 250 255
Phe Val Gln Glu Arg Lys Lys Val Met Ala Gln Thr Gly Glu Ile Arg
260 265 270
Cys Ala Met Asp His Ile Leu Glu Ala Glu Arg Lys Gly Glu Ile Asn
275 280 285
His Asp Asn Val Leu Tyr Ile Val Glu Asn Ile Asn Val Ala Ala Ile
290 295 300
Glu Thr Thr Leu Trp Ser Ile Glu Trp Gly Ile Ala Glu Leu Val Asn
305 310 315 320
His Pro Ala Ile Gln His Lys Leu Arg Glu Glu Leu Ala Ser Val Leu
325 330 335
Gly Ala Gly Val Pro Val Thr Glu Pro Asp Leu Glu Arg Leu Pro Tyr
340 345 350
Leu Gln Ala Ile Val Lys Glu Thr Leu Arg Leu Arg Met Ala Ile Pro
355 360 365
Leu Leu Val Pro His Met Asn Leu Asn Asp Gly Lys Leu Ala Gly Tyr
370 375 380
Asp Ile Pro Ala Glu Ser Lys Ile Leu Val Asn Ala Trp Phe Leu Ala
385 390 395 400
Asn Asp Pro Lys Arg Trp Val Arg Pro Asp Glu Phe Arg Pro Glu Arg
405 410 415
Phe Leu Glu Glu Glu Lys Ser Val Glu Ala His Gly Asn Asp Phe Arg
420 425 430
Phe Val Pro Phe Gly Val Gly Arg Arg Ser Cys Pro Gly Ile Ile Leu
435 440 445
Ala Leu Pro Ile Ile Gly Ile Thr Leu Gly Arg Leu Val Gln Asn Phe
450 455 460
Gln Leu Leu Pro Pro Pro Gly Leu Asp Lys Ile Asp Thr Thr Glu Lys
465 470 475 480
Pro Gly Gln Phe Ser Asn Gln Ile Ala Lys His Ala Thr Ile Val Cys
485 490 495
Lys Pro Leu Glu Ala
500
<210> 122
<211> 505
<212> PRT
<213> 两色高梁()
<220>
<223> 高粱肉桂酸4-羟化酶 (SbC4H)
<400> 122
Met Asp Leu Leu Phe Val Glu Lys Leu Leu Val Gly Leu Phe Ala Ser
1 5 10 15
Val Val Val Ala Ile Ala Val Ser Lys Met Arg Gly Arg Lys Leu Arg
20 25 30
Leu Pro Pro Gly Pro Val Pro Val Pro Ile Phe Gly Asn Trp Leu Gln
35 40 45
Val Gly Asp Asp Leu Asn His Arg Asn Leu Ala Ala Leu Ala Arg Lys
50 55 60
Phe Gly Asp Ile Phe Leu Leu Arg Met Gly Gln Arg Asn Leu Val Val
65 70 75 80
Val Ser Ser Pro Pro Leu Ala Arg Glu Val Leu His Thr Gln Gly Val
85 90 95
Glu Phe Gly Ser Arg Thr Arg Asn Val Val Phe Asp Ile Phe Thr Gly
100 105 110
Glu Gly Gln Asp Met Val Phe Thr Val Tyr Gly Asp His Trp Arg Lys
115 120 125
Met Arg Arg Ile Met Thr Val Pro Phe Phe Thr Asn Lys Val Val Gln
130 135 140
Gln Tyr Arg His Gly Trp Glu Ala Glu Ala Ala Ala Val Val Asp Asp
145 150 155 160
Val Arg Ala Asp Pro Ala Ala Ala Thr Glu Gly Val Val Leu Arg Arg
165 170 175
Arg Leu Gln Leu Met Met Tyr Asn Asn Met Tyr Arg Ile Met Phe Asp
180 185 190
Arg Arg Phe Glu Ser Met Asp Asp Pro Leu Phe Leu Arg Leu Arg Ala
195 200 205
Leu Asn Gly Glu Arg Ser Arg Leu Ala Gln Ser Phe Glu Tyr Asn Tyr
210 215 220
Gly Asp Phe Ile Pro Ile Leu Arg Pro Phe Leu Arg Gly Tyr Leu Arg
225 230 235 240
Ile Cys Lys Glu Val Lys Glu Thr Arg Leu Lys Leu Phe Lys Asp Phe
245 250 255
Phe Leu Glu Glu Arg Lys Lys Leu Ala Ser Thr Lys Ala Val Asp Ser
260 265 270
Asn Gly Leu Lys Cys Ala Ile Asp His Ile Leu Glu Ala Gln Gln Lys
275 280 285
Gly Glu Ile Asn Glu Asp Asn Val Leu Tyr Ile Val Glu Asn Ile Asn
290 295 300
Val Ala Ala Ile Glu Thr Thr Leu Trp Ser Ile Glu Trp Ala Ile Ala
305 310 315 320
Glu Leu Val Asn His Pro Glu Ile Gln Gln Lys Leu Arg Gln Glu Leu
325 330 335
Asp Thr Val Leu Ala Pro Gly Gln Gln Ile Thr Glu Pro Asp Thr His
340 345 350
Asn Leu Pro Tyr Leu Gln Ala Val Ile Lys Glu Thr Leu Arg Leu Arg
355 360 365
Met Ala Ile Pro Leu Leu Val Pro His Met Asn Leu His Asp Ala Lys
370 375 380
Leu Gly Gly Tyr Asp Ile Pro Ala Glu Ser Lys Ile Leu Val Asn Ala
385 390 395 400
Trp Tyr Leu Ala Asn Asn Pro Glu Ser Trp Lys Arg Pro Glu Glu Phe
405 410 415
Arg Pro Glu Arg Phe Leu Glu Glu Glu Lys His Val Glu Ala Asn Gly
420 425 430
Asn Asp Phe Arg Tyr Leu Pro Phe Gly Val Gly Arg Arg Ser Cys Pro
435 440 445
Gly Ile Ile Leu Ala Leu Pro Ile Leu Gly Ile Thr Ile Gly Arg Leu
450 455 460
Val Gln Asn Phe Glu Leu Leu Pro Pro Pro Gly Gln Asp Lys Leu Asp
465 470 475 480
Thr Thr Glu Lys Gly Gly Gln Phe Ser Leu His Ile Leu Lys His Ser
485 490 495
Asn Ile Val Cys Lys Pro Arg Thr Phe
500 505
<210> 123
<211> 506
<212> PRT
<213> 蒺藜状苜蓿()
<220>
<223> 肉桂酸4-羟化酶 (MtC4H)
<400> 123
Met Asp Leu Leu Leu Leu Glu Lys Thr Leu Leu Ala Leu Phe Ile Ala
1 5 10 15
Ala Thr Ile Ala Ile Thr Ile Ser Lys Leu Arg Gly Lys Arg Phe Lys
20 25 30
Leu Pro Pro Gly Pro Ile Pro Val Pro Ile Phe Gly Asn Trp Leu Gln
35 40 45
Val Gly Asp Asp Leu Asn His Arg Asn Leu Thr Asp Tyr Ala Lys Arg
50 55 60
Phe Gly Glu Met Phe Leu Leu Arg Met Gly Gln Arg Asn Leu Val Val
65 70 75 80
Val Ser Ser Pro Glu Leu Ala Lys Glu Val Leu His Thr Gln Gly Val
85 90 95
Glu Phe Gly Ser Arg Thr Arg Asn Val Val Phe Asp Ile Phe Thr Gly
100 105 110
Lys Gly Gln Asp Met Val Phe Thr Val Tyr Gly Glu His Trp Arg Lys
115 120 125
Met Arg Arg Ile Met Thr Val Pro Phe Phe Thr Asn Lys Val Val Gln
130 135 140
Gln Tyr Arg Tyr Gly Trp Glu Ser Glu Ala Glu Ser Val Val Asn Asp
145 150 155 160
Val Lys Asn Asn Ala Glu Ala Ser Ile Gly Gly Ile Val Ile Arg Lys
165 170 175
Arg Leu Gln Leu Met Met Tyr Asn Ile Met Tyr Arg Ile Met Phe Asp
180 185 190
Arg Arg Phe Glu Ser Glu Glu Asp Pro Leu Phe Val Lys Leu Lys Ala
195 200 205
Leu Asn Gly Glu Arg Ser Arg Leu Ala Gln Ser Phe Glu Tyr Asn Tyr
210 215 220
Gly Asp Phe Ile Pro Ile Leu Arg Pro Phe Leu Lys Gly Tyr Leu Lys
225 230 235 240
Val Cys Lys Glu Val Lys Asp Arg Arg Leu Gln Leu Phe Lys Asp Tyr
245 250 255
Phe Val Asp Glu Arg Lys Lys Leu Glu Ser Thr Lys Ser Thr Thr Ser
260 265 270
Asn Asp Gly Leu Lys Cys Ala Ile Asp His Ile Leu Asp Ala Gln Lys
275 280 285
Lys Gly Glu Ile Asn Asp Asp Asn Val Leu Tyr Ile Val Glu Asn Ile
290 295 300
Asn Val Ala Ala Ile Glu Thr Thr Leu Trp Ser Ile Glu Trp Gly Ile
305 310 315 320
Ala Glu Leu Val Asn His Gln Gly Ile Gln Asn Lys Val Arg Glu Glu
325 330 335
Met Asp Arg Val Leu Gly Pro Gly His Gln Val Thr Glu Pro Asp Leu
340 345 350
His Lys Leu Pro Tyr Leu Gln Ala Val Ile Lys Glu Thr Leu Arg Leu
355 360 365
Arg Met Ala Ile Pro Leu Leu Val Pro His Met Asn Leu His Asp Ala
370 375 380
Lys Leu Asn Gly Phe Asp Ile Pro Ala Glu Ser Lys Ile Leu Val Asn
385 390 395 400
Ala Trp Trp Leu Ala Asn Asn Pro Ala His Trp Lys Lys Pro Glu Glu
405 410 415
Phe Arg Pro Glu Arg Phe Leu Glu Glu Glu Ser His Val Glu Ala Asn
420 425 430
Gly Asn Asp Phe Arg Tyr Leu Pro Phe Gly Val Gly Arg Arg Ser Cys
435 440 445
Pro Gly Ile Ile Leu Ala Leu Pro Ile Leu Gly Ile Thr Ile Gly Arg
450 455 460
Leu Val Gln Asn Phe Glu Leu Leu Pro Pro Pro Gly Gln Ser Lys Ile
465 470 475 480
Asp Thr Ser Glu Lys Gly Gly Gln Phe Ser Leu His Ile Leu Lys His
485 490 495
Ser Thr Ile Val Ala Lys Pro Arg Ser Phe
500 505
<210> 124
<211> 501
<212> PRT
<213> 小麦()
<220>
<223> 小麦肉桂酸4-羟化酶 (TaC4H)
<400> 124
Met Asp Val Leu Leu Leu Glu Lys Ala Leu Leu Gly Leu Phe Ala Ala
1 5 10 15
Ala Val Leu Ala Ile Ala Val Ala Lys Leu Thr Gly Lys Arg Phe Arg
20 25 30
Leu Pro Pro Gly Pro Ser Gly Ala Pro Ile Val Gly Asn Trp Leu Gln
35 40 45
Val Gly Asp Asp Leu Asn His Arg Asn Leu Met Gly Leu Ala Lys Arg
50 55 60
Phe Gly Glu Val Phe Leu Leu Arg Met Gly Val Arg Asn Leu Val Val
65 70 75 80
Val Ser Ser Pro Glu Leu Ala Lys Glu Val Leu His Thr Gln Gly Val
85 90 95
Glu Phe Gly Ser Arg Thr Arg Asn Val Val Phe Asp Ile Phe Thr Gly
100 105 110
Lys Gly Gln Asp Met Val Phe Thr Val Tyr Gly Asp His Trp Arg Lys
115 120 125
Met Arg Arg Ile Met Thr Val Pro Phe Phe Thr Asn Lys Val Val Ala
130 135 140
Gln Asn Arg Val Gly Trp Glu Glu Glu Ala Arg Leu Val Val Glu Asp
145 150 155 160
Leu Lys Ala Asp Pro Ala Ala Ala Thr Ala Gly Val Val Val Arg Arg
165 170 175
Arg Leu Gln Leu Met Met Tyr Asn Asp Met Phe Arg Ile Met Phe Asp
180 185 190
Arg Arg Phe Glu Ser Val Ala Asp Pro Leu Phe Asn Gln Leu Lys Ala
195 200 205
Leu Asn Ala Glu Arg Ser Ile Leu Ser Gln Ser Phe Asp Tyr Asn Tyr
210 215 220
Gly Asp Phe Ile Pro Val Leu Arg Pro Phe Leu Arg Arg Tyr Leu Asn
225 230 235 240
Arg Cys Thr Asn Leu Lys Thr Lys Arg Met Lys Val Phe Glu Asp His
245 250 255
Phe Val Gln Gln Arg Lys Glu Ala Leu Glu Lys Thr Gly Glu Ile Arg
260 265 270
Cys Ala Met Asp His Ile Leu Glu Ala Glu Arg Lys Gly Glu Ile Asn
275 280 285
His Asp Asn Val Leu Tyr Ile Val Glu Asn Ile Asn Val Ala Ala Ile
290 295 300
Glu Thr Thr Leu Trp Ser Ile Glu Trp Gly Leu Ala Glu Leu Val Asn
305 310 315 320
His Pro Glu Ile Gln Gln Lys Leu Arg Glu Glu Ile Val Ala Val Leu
325 330 335
Gly Ala Gly Val Ala Val Thr Glu Pro Asp Leu Glu Arg Leu Pro Tyr
340 345 350
Leu Gln Ser Val Val Lys Glu Thr Leu Arg Leu Arg Met Ala Ile Pro
355 360 365
Leu Leu Val Pro His Met Asn Leu Ser Asp Ala Lys Leu Ala Gly Tyr
370 375 380
Asp Ile Pro Ala Glu Ser Lys Ile Leu Val Asn Ala Trp Phe Leu Ala
385 390 395 400
Asn Asp Pro Lys Arg Trp Val Arg Ala Asp Glu Phe Arg Pro Glu Arg
405 410 415
Phe Leu Glu Glu Glu Lys Ala Val Glu Ala His Gly Asn Asp Phe Arg
420 425 430
Phe Val Pro Phe Gly Val Gly Arg Arg Ser Cys Pro Gly Ile Ile Leu
435 440 445
Ala Leu Pro Ile Ile Gly Ile Thr Leu Gly Arg Leu Val Gln Asn Phe
450 455 460
Gln Leu Leu Pro Pro Pro Gly Gln Asp Lys Ile Asp Thr Thr Glu Lys
465 470 475 480
Pro Gly Gln Phe Thr Asn Gln Ile Leu Lys His Ala Thr Ile Val Cys
485 490 495
Lys Pro Leu Glu Ala
500
<210> 125
<211> 506
<212> PRT
<213> 大豆()
<220>
<223> 大豆肉桂酸4-羟化酶 (GmC4H)
<400> 125
Met Asp Leu Leu Leu Leu Glu Lys Thr Leu Ile Gly Leu Phe Leu Ala
1 5 10 15
Ala Val Val Ala Ile Ala Val Ser Thr Leu Arg Gly Arg Lys Phe Lys
20 25 30
Leu Pro Pro Gly Pro Leu Pro Val Pro Ile Phe Gly Asn Trp Leu Gln
35 40 45
Val Gly Asp Asp Leu Asn His Arg Asn Leu Thr Asp Leu Ala Lys Lys
50 55 60
Phe Gly Asp Ile Phe Leu Leu Arg Met Gly Gln Arg Asn Leu Val Val
65 70 75 80
Val Ser Ser Pro Glu Leu Ala Lys Glu Val Leu His Thr Gln Gly Val
85 90 95
Glu Phe Gly Ser Arg Thr Arg Asn Val Val Phe Asp Ile Phe Thr Gly
100 105 110
Lys Gly Gln Asp Met Val Phe Thr Val Tyr Gly Glu His Trp Arg Lys
115 120 125
Met Arg Arg Ile Met Thr Val Pro Phe Phe Thr Asn Lys Val Val Gln
130 135 140
Gln Tyr Arg His Gly Trp Glu Ser Glu Ala Ala Ala Val Val Glu Asp
145 150 155 160
Val Lys Lys Asn Pro Asp Ala Ala Val Ser Gly Thr Val Ile Arg Arg
165 170 175
Arg Leu Gln Leu Met Met Tyr Asn Asn Met Tyr Arg Ile Met Phe Asp
180 185 190
Arg Arg Phe Glu Ser Glu Glu Asp Pro Ile Phe Gln Arg Leu Arg Ala
195 200 205
Leu Asn Gly Glu Arg Ser Arg Leu Ala Gln Ser Phe Glu Tyr Asn Tyr
210 215 220
Gly Asp Phe Ile Pro Ile Leu Arg Pro Phe Leu Lys Gly Tyr Leu Lys
225 230 235 240
Ile Cys Lys Glu Val Lys Glu Thr Arg Leu Lys Leu Phe Lys Asp Tyr
245 250 255
Phe Val Asp Glu Arg Lys Lys Leu Gly Ser Thr Lys Ser Thr Asn Asn
260 265 270
Asn Asn Glu Leu Lys Cys Ala Ile Asp His Ile Leu Asp Ala Gln Arg
275 280 285
Lys Gly Glu Ile Asn Glu Asp Asn Val Leu Tyr Ile Val Glu Asn Ile
290 295 300
Asn Val Ala Ala Ile Glu Thr Thr Leu Trp Ser Ile Glu Trp Gly Ile
305 310 315 320
Ala Glu Leu Val Asn His Pro Glu Ile Gln Gln Lys Leu Arg Asp Glu
325 330 335
Ile Asp Arg Val Leu Gly Ala Gly His Gln Val Thr Glu Pro Asp Ile
340 345 350
Gln Lys Leu Pro Tyr Leu Gln Ala Val Val Lys Glu Thr Leu Arg Leu
355 360 365
Arg Met Ala Ile Pro Leu Leu Val Pro His Met Asn Leu His Asp Ala
370 375 380
Lys Leu Gly Gly Tyr Asp Ile Pro Ala Glu Ser Arg Ile Leu Val Asn
385 390 395 400
Ala Trp Trp Leu Ala Asn Asn Pro Ala His Trp Lys Lys Pro Glu Glu
405 410 415
Phe Arg Pro Glu Arg Phe Phe Glu Glu Glu Ser Leu Val Glu Ala Asn
420 425 430
Gly Asn Asp Phe Arg Tyr Leu Pro Phe Gly Val Gly Arg Arg Ser Cys
435 440 445
Pro Gly Ile Ile Leu Ala Leu Pro Ile Leu Gly Ile Thr Leu Gly Arg
450 455 460
Leu Val Gln Asn Phe Glu Leu Leu Pro Pro Pro Gly Gln Ser Gln Ile
465 470 475 480
Asp Thr Ser Glu Lys Gly Gly Gln Phe Ser Leu His Ile Leu Lys His
485 490 495
Ser Thr Ile Val Ala Lys Pro Arg Ser Phe
500 505
<210> 126
<211> 505
<212> PRT
<213> 红花烟草()
<220>
<223> 烟草肉桂酸4-羟化酶 (NtC4H)
<400> 126
Met Asp Leu Leu Leu Leu Glu Lys Thr Leu Ile Gly Leu Phe Phe Ala
1 5 10 15
Ile Leu Ile Ala Leu Ile Val Ser Lys Leu Arg Ser Lys Arg Phe Lys
20 25 30
Leu Pro Pro Gly Pro Ile Pro Val Pro Val Phe Gly Asn Trp Leu Gln
35 40 45
Val Gly Asp Asp Leu Asn His Arg Asn Leu Thr Asp Tyr Ala Lys Lys
50 55 60
Phe Gly Asp Leu Phe Leu Leu Arg Met Gly Gln Arg Asn Leu Val Val
65 70 75 80
Val Ser Ser Pro Glu Leu Ala Lys Glu Val Leu His Thr Gln Gly Val
85 90 95
Glu Phe Gly Ser Arg Thr Arg Asn Val Val Phe Asp Ile Phe Thr Gly
100 105 110
Lys Gly Gln Asp Met Val Phe Thr Val Tyr Gly Glu His Trp Arg Lys
115 120 125
Met Arg Arg Ile Met Thr Val Pro Phe Phe Thr Asn Lys Val Val Gln
130 135 140
Gln Tyr Arg Gly Gly Trp Glu Phe Glu Val Ala Ser Val Ile Glu Asp
145 150 155 160
Val Lys Lys Asn Pro Glu Ser Ala Thr Asn Gly Ile Val Leu Arg Arg
165 170 175
Arg Leu Gln Leu Met Met Tyr Asn Asn Met Phe Arg Ile Met Phe Asp
180 185 190
Arg Arg Phe Glu Ser Glu Asp Asp Pro Leu Phe Val Lys Leu Lys Ala
195 200 205
Leu Asn Gly Glu Arg Ser Arg Leu Ala Gln Ser Phe Glu Tyr Asn Tyr
210 215 220
Gly Asp Phe Ile Pro Ile Leu Arg Pro Phe Leu Arg Gly Tyr Leu Lys
225 230 235 240
Ile Cys Lys Glu Val Lys Glu Lys Arg Leu Gln Leu Phe Lys Asp Tyr
245 250 255
Phe Val Asp Glu Arg Lys Lys Leu Ser Asn Thr Lys Ser Ser Asp Ser
260 265 270
Asn Ala Leu Lys Cys Ala Ile Asp His Ile Leu Glu Ala Gln Gln Lys
275 280 285
Gly Glu Ile Asn Glu Asp Asn Val Leu Tyr Ile Val Glu Asn Ile Asn
290 295 300
Val Ala Ala Ile Glu Thr Thr Leu Trp Ser Ile Glu Trp Gly Ile Ala
305 310 315 320
Glu Leu Val Asn His Pro His Ile Gln Lys Lys Leu Arg Asp Glu Ile
325 330 335
Asp Thr Val Leu Gly Pro Gly Val Gln Val Thr Glu Pro Asp Thr His
340 345 350
Lys Leu Pro Tyr Leu Gln Ala Val Ile Lys Glu Ala Leu Arg Leu Arg
355 360 365
Met Ala Ile Pro Leu Leu Val Pro His Met Asn Leu His Asp Ala Lys
370 375 380
Leu Gly Gly Phe Asp Ile Pro Ala Glu Ser Lys Ile Leu Val Asn Ala
385 390 395 400
Trp Trp Leu Ala Asn Asn Pro Ala His Trp Lys Lys Pro Glu Glu Phe
405 410 415
Arg Pro Glu Arg Phe Phe Glu Glu Glu Lys His Val Glu Ala Asn Gly
420 425 430
Asn Asp Phe Arg Tyr Leu Pro Phe Gly Val Gly Arg Arg Ser Cys Pro
435 440 445
Gly Ile Ile Leu Ala Leu Pro Ile Leu Gly Ile Thr Leu Gly Arg Leu
450 455 460
Val Gln Asn Phe Glu Leu Leu Pro Pro Pro Gly Gln Ser Lys Leu Asp
465 470 475 480
Thr Thr Glu Lys Gly Gly Gln Phe Ser Leu His Ile Leu Lys His Ser
485 490 495
Thr Ile Val Leu Lys Pro Arg Ser Phe
500 505
<210> 127
<211> 505
<212> PRT
<213> 马铃薯()
<220>
<223> 马铃薯肉桂酸4-羟化酶 (StC4H)
<400> 127
Met Asp Leu Leu Leu Leu Glu Lys Thr Leu Ile Gly Leu Phe Phe Ala
1 5 10 15
Ile Leu Ile Ala Ile Ile Val Ser Lys Leu Arg Ser Lys Arg Phe Lys
20 25 30
Leu Pro Pro Gly Pro Ile Pro Val Pro Val Phe Gly Asn Trp Leu Gln
35 40 45
Val Gly Asp Asp Leu Asn His Arg Asn Leu Thr Glu Tyr Ala Lys Lys
50 55 60
Phe Gly Asp Val Phe Leu Leu Arg Met Gly Gln Arg Asn Leu Val Val
65 70 75 80
Val Ser Ser Pro Glu Leu Ala Lys Glu Val Leu His Thr Gln Gly Val
85 90 95
Glu Phe Gly Ser Arg Thr Arg Asn Val Val Phe Asp Ile Phe Thr Gly
100 105 110
Lys Gly Gln Asp Met Val Phe Thr Val Tyr Gly Glu His Trp Arg Lys
115 120 125
Met Arg Arg Ile Met Thr Val Pro Phe Phe Thr Asn Lys Val Val Gln
130 135 140
Gln Tyr Arg Gly Gly Trp Glu Ser Glu Ala Ala Ser Val Val Glu Asp
145 150 155 160
Val Lys Lys Asn Pro Glu Ser Ala Thr Asn Gly Ile Val Leu Arg Lys
165 170 175
Arg Leu Gln Leu Met Met Tyr Asn Asn Met Phe Arg Ile Met Phe Asp
180 185 190
Arg Arg Phe Glu Ser Glu Asp Asp Pro Leu Phe Val Lys Leu Arg Ala
195 200 205
Leu Asn Gly Glu Arg Ser Arg Leu Ala Gln Ser Phe Glu Tyr Asn Tyr
210 215 220
Gly Asp Phe Ile Pro Ile Leu Arg Pro Phe Leu Arg Gly Tyr Leu Lys
225 230 235 240
Ile Cys Lys Glu Val Lys Glu Lys Arg Leu Lys Leu Phe Lys Asp Tyr
245 250 255
Phe Val Asp Glu Arg Lys Lys Leu Ala Asn Thr Lys Ser Met Asp Ser
260 265 270
Asn Ala Leu Lys Cys Ala Ile Asp His Ile Leu Glu Ala Gln Gln Lys
275 280 285
Gly Glu Ile Asn Glu Asp Asn Val Leu Tyr Ile Val Glu Asn Ile Asn
290 295 300
Val Ala Ala Ile Glu Thr Thr Leu Trp Ser Ile Glu Trp Gly Ile Ala
305 310 315 320
Glu Leu Val Asn His Pro His Ile Gln Lys Lys Leu Arg Asp Glu Ile
325 330 335
Asp Thr Val Leu Gly Pro Gly Met Gln Val Thr Glu Pro Asp Met Pro
340 345 350
Lys Leu Pro Tyr Leu Gln Ala Val Ile Lys Glu Thr Leu Arg Leu Arg
355 360 365
Met Ala Ile Pro Leu Leu Val Pro His Met Asn Leu His Asp Ala Lys
370 375 380
Leu Ala Gly Tyr Asp Ile Pro Ala Glu Ser Lys Ile Leu Val Asn Ala
385 390 395 400
Trp Trp Leu Ala Asn Asn Pro Ala His Trp Lys Lys Pro Glu Glu Phe
405 410 415
Arg Pro Glu Arg Phe Phe Glu Glu Glu Lys His Val Glu Ala Asn Gly
420 425 430
Asn Asp Phe Arg Phe Leu Pro Phe Gly Val Gly Arg Arg Ser Cys Pro
435 440 445
Gly Ile Ile Leu Ala Leu Pro Ile Leu Gly Ile Thr Leu Gly Arg Leu
450 455 460
Val Gln Asn Phe Glu Met Leu Pro Pro Pro Gly Gln Ser Lys Leu Asp
465 470 475 480
Thr Ser Glu Lys Gly Gly Gln Phe Ser Leu His Ile Leu Lys His Ser
485 490 495
Thr Ile Val Met Lys Pro Arg Ser Phe
500 505
<210> 128
<211> 507
<212> PRT
<213> 绿竹()
<220>
<223> 竹肉桂酸4-羟化酶 (BoC4H)
<400> 128
Met Asp Leu Leu Phe Leu Glu Lys Leu Leu Val Gly Leu Phe Ala Ser
1 5 10 15
Val Val Val Ala Ile Ala Val Ser Lys Ile Arg Gly Arg Lys Leu Arg
20 25 30
Leu Pro Pro Gly Pro Leu Pro Val Pro Ile Phe Gly Asn Trp Leu Gln
35 40 45
Val Gly Asp Asp Leu Asn His Arg Asn Leu Ala Ala Leu Ala Arg Lys
50 55 60
Phe Gly Glu Ile Phe Leu Leu Arg Met Gly Gln Arg Asn Leu Val Val
65 70 75 80
Val Ser Ser Pro Pro Leu Ala Arg Glu Val Leu His Thr Gln Gly Val
85 90 95
Glu Phe Gly Ser Arg Thr Arg Asn Val Val Phe Asp Ile Phe Thr Gly
100 105 110
Lys Gly Gln Asp Met Val Phe Thr Val Tyr Gly Asp His Trp Arg Lys
115 120 125
Met Arg Arg Ile Met Thr Val Pro Phe Phe Thr Asn Lys Val Val Gln
130 135 140
Gln Tyr His Pro Gly Trp Glu Ala Glu Ala Ala Ala Val Val Asp Ala
145 150 155 160
Val Arg Ala Asp Pro Lys Ala Ala Thr Glu Gly Val Val Leu Arg Arg
165 170 175
His Leu Gln Leu Met Met Tyr Asn Asn Met Tyr Arg Ile Met Phe Asp
180 185 190
Arg Arg Phe Glu Ser Met Asp Asp Pro Leu Phe Leu Arg Leu Arg Ala
195 200 205
Leu Asn Gly Glu Arg Ser Arg Leu Ala Gln Ser Phe Glu Tyr Asn Tyr
210 215 220
Gly Asp Phe Ile Pro Ile Leu Arg Pro Phe Leu Arg Gly Tyr Leu Lys
225 230 235 240
Thr Cys Lys Glu Val Lys Glu Thr Arg Leu Lys Leu Phe Lys Asp Phe
245 250 255
Phe Leu Glu Glu Arg Lys Lys Leu Ala Ser Ser Lys Pro Met Asp Ser
260 265 270
Ser Gly Leu Lys Cys Ala Ile Asp His Ile Leu Glu Ala Gln Gln Lys
275 280 285
Gly Glu Ile Asn Glu Asp Asn Val Leu Tyr Ile Val Glu Asn Ile Asn
290 295 300
Val Ala Ala Ile Glu Thr Thr Leu Trp Ser Ile Glu Trp Ala Ile Gly
305 310 315 320
Glu Leu Val Asn His Pro Glu Ile Gln Arg Lys Leu Arg His Glu Leu
325 330 335
Asp Ala Val Leu Gly Pro Gly His Gln Ile Thr Glu Pro Asp Thr His
340 345 350
Lys Leu Pro Tyr Leu Gln Ala Val Ile Lys Glu Thr Leu Arg Leu Arg
355 360 365
Met Ala Ile Pro Leu Leu Val Pro His Met Asn Leu His Asp Ala Lys
370 375 380
Leu Ser Gly Tyr Asp Ile Pro Ala Glu Ser Lys Ile Leu Val Asn Ala
385 390 395 400
Trp Tyr Leu Ala Asn Asn Pro Asp Gln Trp Lys Arg Pro Glu Glu Leu
405 410 415
Arg Pro Glu Arg Phe Leu Glu Glu Glu Lys His Val Glu Ala Asn Gly
420 425 430
Asn Asp Phe Arg Tyr Leu Pro Phe Gly Val Gly Arg Arg Ser Cys Pro
435 440 445
Gly Ile Ile Leu Ala Leu Pro Ile Leu Gly Ile Thr Ile Gly Arg Leu
450 455 460
Val Gln Asn Phe Glu Leu Leu Pro Pro Pro Gly Gln Asp Lys Leu Asp
465 470 475 480
Thr Ala Glu Lys Gly Gly Gln Phe Ser Leu His Ile Leu Lys His Ser
485 490 495
Asn Ile Val Ala Lys Pro Arg Ala Leu Glu Leu
500 505
<210> 129
<211> 505
<212> PRT
<213> 甘蓝型油菜()
<220>
<223> 肉桂酸4-羟化酶 (BnC4H1)
<400> 129
Met Asp Leu Leu Leu Leu Glu Lys Ser Leu Ile Ala Val Phe Ala Ala
1 5 10 15
Val Val Leu Ala Thr Val Ile Ser Lys Leu Arg Gly Lys Lys Leu Lys
20 25 30
Leu Pro Pro Gly Pro Met Pro Ile Pro Ile Phe Gly Asn Trp Leu Gln
35 40 45
Val Gly Asp Asp Leu Asn His Arg Asn Leu Val Asp Tyr Ala Lys Lys
50 55 60
Phe Gly Asp Leu Phe Leu Leu Arg Met Gly Gln Arg Asn Leu Val Val
65 70 75 80
Val Ser Ser Pro Asn Leu Thr Lys Glu Val Leu His Thr Gln Gly Val
85 90 95
Glu Phe Gly Ser Arg Thr Arg Asn Val Val Phe Asp Ile Phe Thr Gly
100 105 110
Lys Gly Gln Asp Met Val Phe Thr Val Tyr Gly Glu His Trp Arg Lys
115 120 125
Met Arg Arg Ile Met Thr Val Pro Phe Phe Thr Asn Lys Val Val Gln
130 135 140
Gln Asn Arg Glu Gly Trp Glu Phe Glu Ala Ala Ser Val Val Glu Asp
145 150 155 160
Val Lys Lys Asn Pro Asp Ser Ala Thr Lys Gly Ile Val Leu Arg Lys
165 170 175
Arg Leu Gln Leu Met Met Tyr Asn Asn Met Phe Arg Ile Met Phe Asp
180 185 190
Arg Arg Phe Asp Ser Glu Asp Asp Pro Leu Phe Ile Arg Leu Lys Ala
195 200 205
Leu Asn Gly Glu Arg Ser Arg Leu Ala Gln Ser Phe Glu Tyr Asn Tyr
210 215 220
Gly Asp Phe Ile Pro Ile Leu Arg Pro Phe Leu Arg Gly Tyr Leu Lys
225 230 235 240
Ile Cys Gln Asp Val Lys Asp Arg Arg Leu Ala Leu Phe Lys Lys Tyr
245 250 255
Phe Val Asp Glu Arg Lys Gln Ile Ala Ser Ser Lys Pro Thr Gly Ser
260 265 270
Glu Gly Leu Lys Cys Ala Ile Asp His Ile Leu Val Ala Gln Gln Lys
275 280 285
Gly Glu Ile Asn Glu Asp Asn Val Leu Tyr Ile Val Glu Asn Ile Asn
290 295 300
Val Ala Ala Ile Glu Thr Thr Leu Trp Ser Ile Glu Trp Gly Ile Ala
305 310 315 320
Glu Leu Val Asn His Pro Glu Ile Gln Ser Lys Leu Arg Asn Glu Ile
325 330 335
Asp Thr Val Leu Gly Pro Gly Val Gln Val Thr Glu Pro Glu Leu His
340 345 350
Lys Leu Pro Tyr Leu Gln Ala Val Ile Lys Glu Thr Leu Arg Leu Arg
355 360 365
Met Ala Ile Pro Leu Leu Val Pro His Met Asn Leu Asn Asp Ala Lys
370 375 380
Leu Ala Gly Tyr Asp Ile Pro Ala Glu Ser Lys Ile Leu Val Asn Ala
385 390 395 400
Trp Trp Leu Ala Asn Asn Pro Glu Ser Trp Lys Lys Pro Glu Glu Phe
405 410 415
Arg Pro Glu Arg Phe Phe Glu Glu Glu Ala His Val Glu Ala Asn Gly
420 425 430
Asn Asp Phe Lys Tyr Val Pro Phe Gly Val Gly Arg Arg Ser Cys Pro
435 440 445
Gly Ile Ile Leu Ala Leu Pro Ile Leu Gly Ile Thr Ile Gly Arg Leu
450 455 460
Val Gln Asn Phe Glu Leu Leu Pro Pro Pro Gly Gln Ser Lys Val Asp
465 470 475 480
Thr Ser Glu Lys Gly Gly Gln Phe Ser Leu His Ile Leu His His Ser
485 490 495
Thr Ile Val Met Lys Pro Arg Ser Phe
500 505
<210> 130
<211> 505
<212> PRT
<213> 向日葵()
<220>
<223> 向日葵肉桂酸4-羟化酶 (HaC4H)
<400> 130
Met Asp Leu Leu Leu Ile Glu Lys Thr Leu Val Ala Leu Phe Ala Ala
1 5 10 15
Ile Ile Gly Ala Ile Leu Ile Ser Lys Leu Arg Gly Lys Lys Phe Lys
20 25 30
Leu Pro Pro Gly Pro Ile Pro Val Pro Ile Phe Gly Asn Trp Leu Gln
35 40 45
Val Gly Asp Asp Leu Asn His Arg Asn Leu Thr Asp Leu Ala Lys Arg
50 55 60
Phe Gly Glu Ile Leu Leu Leu Arg Met Gly Gln Arg Asn Leu Val Val
65 70 75 80
Val Ser Ser Pro Glu Leu Ala Lys Glu Val Leu His Thr Gln Gly Val
85 90 95
Glu Phe Gly Ser Arg Thr Arg Asn Val Val Phe Asp Ile Phe Thr Gly
100 105 110
Lys Gly Gln Asp Met Val Phe Thr Val Tyr Gly Glu His Trp Arg Lys
115 120 125
Met Arg Arg Ile Met Thr Val Pro Phe Phe Thr Asn Lys Val Val Gln
130 135 140
Gln Tyr Arg Tyr Gly Trp Glu Ala Glu Ala Ala Ala Val Val Asp Asp
145 150 155 160
Val Lys Lys Asn Pro Ala Ala Ala Thr Glu Gly Ile Val Ile Arg Arg
165 170 175
Arg Leu Gln Leu Met Met Tyr Asn Asn Met Phe Arg Ile Met Phe Asp
180 185 190
Arg Arg Phe Glu Ser Glu Asp Asp Pro Leu Phe Leu Lys Leu Lys Ala
195 200 205
Leu Asn Gly Glu Arg Ser Arg Leu Ala Gln Ser Phe Glu Tyr Asn Tyr
210 215 220
Gly Asp Phe Ile Pro Ile Leu Arg Pro Phe Leu Arg Asn Tyr Leu Lys
225 230 235 240
Leu Cys Lys Glu Val Lys Asp Lys Arg Ile Gln Leu Phe Lys Asp Tyr
245 250 255
Phe Val Asp Glu Arg Lys Lys Ile Gly Ser Thr Lys Lys Met Asp Asn
260 265 270
Asn Gln Leu Lys Cys Ala Ile Asp His Ile Leu Glu Ala Lys Glu Lys
275 280 285
Gly Glu Ile Asn Glu Asp Asn Val Leu Tyr Ile Val Glu Asn Ile Asn
290 295 300
Val Ala Ala Ile Glu Thr Thr Leu Trp Ser Ile Glu Trp Gly Ile Ala
305 310 315 320
Glu Leu Val Asn His Pro Glu Ile Gln Ala Lys Leu Arg His Glu Leu
325 330 335
Asp Thr Lys Leu Gly Pro Gly Val Gln Ile Thr Glu Pro Asp Val Gln
340 345 350
Asn Leu Pro Tyr Leu Gln Ala Val Val Lys Glu Thr Leu Arg Leu Arg
355 360 365
Met Ala Ile Pro Leu Leu Val Pro His Met Asn Leu His Asp Ala Lys
370 375 380
Leu Gly Gly Phe Asp Ile Pro Ala Glu Ser Lys Ile Leu Val Asn Ala
385 390 395 400
Trp Trp Leu Ala Asn Asn Pro Asp Gln Trp Lys Lys Pro Glu Glu Phe
405 410 415
Arg Pro Glu Arg Phe Leu Glu Glu Glu Ala Lys Val Glu Ala Asn Gly
420 425 430
Asn Asp Phe Arg Tyr Leu Pro Phe Gly Val Gly Arg Arg Ser Cys Pro
435 440 445
Gly Ile Ile Leu Ala Leu Pro Ile Leu Gly Ile Thr Ile Gly Arg Leu
450 455 460
Val Gln Asn Phe Glu Leu Leu Pro Pro Pro Gly Gln Ser Lys Ile Asp
465 470 475 480
Thr Asp Glu Lys Gly Gly Gln Phe Ser Leu His Ile Leu Lys His Ser
485 490 495
Thr Ile Val Ala Lys Pro Arg Ser Phe
500 505
<210> 131
<211> 505
<212> PRT
<213> 蓖麻()
<220>
<223> 肉桂酸4-羟化酶 (RcC4H)
<400> 131
Met Asp Pro Leu Leu Leu Glu Lys Thr Leu Leu Gly Leu Phe Ala Ala
1 5 10 15
Val Ile Val Ala Ile Val Ile Ser Lys Leu Arg Gly Lys Arg Phe Lys
20 25 30
Leu Pro Pro Gly Pro Leu Pro Val Pro Val Phe Gly Asn Trp Leu Gln
35 40 45
Val Gly Asp Asp Leu Asn Gln Arg Asn Leu Ala Asp Leu Ala Lys Lys
50 55 60
Phe Gly Asp Ile Leu Leu Leu Arg Met Gly Gln Arg Asn Leu Val Val
65 70 75 80
Val Ser Ser Pro Asp Leu Ala Lys Glu Val Leu His Thr Gln Gly Val
85 90 95
Glu Phe Gly Ser Arg Thr Arg Asn Val Val Phe Asp Ile Phe Thr Gly
100 105 110
Lys Gly Gln Asp Met Val Phe Thr Val Tyr Gly Glu His Trp Arg Lys
115 120 125
Met Arg Arg Ile Met Thr Val Pro Phe Phe Thr Asn Lys Val Val Gln
130 135 140
Gln Tyr Arg Tyr Gly Trp Glu Asp Glu Ala Ala Arg Val Val Glu Asp
145 150 155 160
Val Lys Lys Asn Pro Glu Ser Ala Thr Asn Gly Ile Val Leu Arg Arg
165 170 175
Arg Leu Gln Leu Met Met Tyr Asn Asn Met Tyr Arg Ile Met Phe Asp
180 185 190
Arg Arg Phe Glu Ser Glu Glu Asp Pro Leu Phe Val Lys Leu Arg Ala
195 200 205
Leu Asn Gly Glu Arg Ser Arg Leu Ala Gln Ser Phe Glu Tyr Asn Tyr
210 215 220
Gly Asp Phe Ile Pro Ile Leu Arg Pro Phe Leu Arg Gly Tyr Leu Lys
225 230 235 240
Ile Cys Lys Glu Val Lys Glu Arg Arg Leu Gln Leu Phe Lys Asp Tyr
245 250 255
Phe Val Asp Glu Arg Lys Lys Leu Gly Ser Thr Lys Ser Met Asn Asn
260 265 270
Glu Gly Leu Lys Cys Ala Ile Asp His Ile Leu Asp Ala Gln Gln Lys
275 280 285
Gly Glu Ile Asn Glu Asp Asn Val Leu Tyr Ile Val Glu Asn Ile Asn
290 295 300
Val Ala Ala Ile Glu Thr Thr Leu Trp Ser Ile Glu Trp Gly Ile Ala
305 310 315 320
Glu Leu Val Asn His Pro Glu Ile Gln Lys Lys Leu Arg Asp Glu Leu
325 330 335
Asp Thr Val Leu Gly Pro Gly Asn Gln Ile Thr Glu Pro Asp Thr Tyr
340 345 350
Lys Leu Pro Tyr Leu Gln Ala Val Val Lys Glu Thr Leu Arg Leu Arg
355 360 365
Met Ala Ile Pro Leu Leu Val Pro His Met Asn Leu His Asp Ala Lys
370 375 380
Leu Ser Gly Tyr Asp Ile Pro Ala Glu Ser Lys Ile Leu Val Asn Ala
385 390 395 400
Trp Trp Leu Ala Asn Asn Pro Ala Asn Trp Lys Asn Pro Glu Glu Phe
405 410 415
Arg Pro Glu Arg Phe Leu Glu Glu Glu Ser Lys Val Glu Ala Asn Gly
420 425 430
Asn Asp Phe Arg Tyr Leu Pro Phe Gly Val Gly Arg Arg Ser Cys Pro
435 440 445
Gly Ile Ile Leu Ala Leu Pro Ile Leu Gly Ile Thr Leu Gly Arg Leu
450 455 460
Val Gln Asn Phe Glu Leu Leu Pro Pro Pro Gly Gln Ser Lys Leu Asp
465 470 475 480
Thr Thr Glu Lys Gly Gly Gln Phe Ser Leu His Ile Leu Lys His Ser
485 490 495
Thr Ile Val Ala Lys Pro Arg Ser Phe
500 505
<210> 132
<211> 505
<212> PRT
<213> 葡萄()
<220>
<223> 葡萄肉桂酸4-羟化酶 (VvC4H)
<400> 132
Met Asp Leu Ile Leu Ile Glu Lys Ala Leu Leu Ala Val Phe Cys Ala
1 5 10 15
Ile Ile Leu Ala Ile Thr Ile Ser Lys Leu Leu Gly Lys Lys Leu Lys
20 25 30
Leu Pro Pro Gly Pro Leu Pro Val Pro Val Phe Gly Asn Trp Leu Gln
35 40 45
Val Gly Asp Asp Leu Asn His Leu Asn Leu Ser Asp Leu Ala Lys Lys
50 55 60
Phe Gly Asp Ile Phe Met Leu Arg Met Gly Gln Arg Asn Leu Val Val
65 70 75 80
Val Ser Ser Pro Asp Leu Ala Lys Asp Val Leu His Thr Gln Gly Val
85 90 95
Glu Phe Gly Ser Arg Thr Arg Asn Val Val Phe Asp Ile Phe Thr Gly
100 105 110
Lys Gly Gln Asp Met Val Phe Thr Val Tyr Gly Glu His Trp Arg Lys
115 120 125
Met Arg Arg Ile Met Thr Val Pro Phe Phe Thr Asn Lys Val Val Gln
130 135 140
Gln Tyr Arg Val Gly Trp Glu Asp Glu Ala Ala Arg Val Val Glu Asp
145 150 155 160
Val Lys Lys Asn Pro Glu Ala Ser Thr Asn Gly Ile Val Leu Arg Arg
165 170 175
Arg Leu Gln Leu Met Met Tyr Asn Asn Met Tyr Arg Ile Met Phe Asp
180 185 190
Arg Arg Phe Asp Ser Glu Glu Asp Pro Leu Phe Val Lys Leu Lys Ala
195 200 205
Leu Asn Gly Glu Arg Ser Arg Leu Ala Gln Ser Phe Glu Tyr Asn Tyr
210 215 220
Gly Asp Phe Ile Pro Ile Leu Arg Pro Phe Leu Arg Gly Tyr Leu Lys
225 230 235 240
Ile Cys Lys Glu Val Lys Glu Arg Arg Leu Gln Leu Phe Lys Asp His
245 250 255
Phe Leu Glu Glu Arg Lys Lys Leu Ala Ser Thr Lys Ser Thr Asp His
260 265 270
Asn Ser Leu Lys Cys Ala Val Asp His Ile Leu Asp Ala Gln Gln Lys
275 280 285
Gly Glu Ile Asn Glu Asp Asn Val Leu Tyr Ile Val Glu Asn Ile Asn
290 295 300
Val Ala Ala Ile Glu Thr Thr Leu Trp Ser Ile Glu Trp Gly Ile Ala
305 310 315 320
Glu Leu Val Asn His Pro His Ile Gln Lys Lys Leu Arg Asp Glu Leu
325 330 335
Asn Thr Val Leu Gly Pro Gly Val Gln Val Thr Glu Pro Asp Ile Gln
340 345 350
Lys Leu Pro Tyr Leu Gln Ala Val Ile Lys Glu Thr Leu Arg Leu Arg
355 360 365
Met Ala Ile Pro Leu Leu Val Pro His Met Asn Leu Asn Asp Ala Lys
370 375 380
Leu Gly Gly Tyr Asp Ile Pro Ala Glu Ser Lys Ile Leu Val Asn Ala
385 390 395 400
Trp Trp Leu Ala Asn Asp Ser Ser Lys Trp Lys Lys Pro Glu Glu Phe
405 410 415
Arg Pro Glu Arg Phe Leu Glu Glu Glu Ser Lys Val Glu Ala Asn Gly
420 425 430
Asn Asp Phe Arg Tyr Leu Pro Phe Gly Val Gly Arg Arg Ser Cys Pro
435 440 445
Gly Ile Ile Leu Ala Leu Pro Ile Leu Gly Ile Thr Ile Gly Arg Leu
450 455 460
Val Gln Asn Phe Glu Leu Leu Pro Pro Pro Gly Gln Ala Lys Leu Asp
465 470 475 480
Thr Thr Gly Lys Gly Gly Gln Phe Ser Leu His Ile Leu Lys His Ser
485 490 495
Thr Ile Val Ala Arg Pro Ile Glu Ala
500 505
<210> 133
<211> 505
<212> PRT
<213> 一品红()
<220>
<223> 一品红肉桂酸4-羟化酶 (EpC4H)
<400> 133
Met Asp Pro Leu Leu Leu Glu Lys Thr Leu Leu Gly Leu Phe Ala Ala
1 5 10 15
Val Ile Val Ala Ile Val Ile Ser Lys Leu Arg Gly Lys Arg Phe Lys
20 25 30
Leu Pro Pro Gly Pro Leu Pro Val Pro Val Phe Gly Asn Trp Leu Gln
35 40 45
Val Gly Asp Asp Leu Asn Gln Arg Asn Leu Ala Asp Leu Ala Lys Lys
50 55 60
Phe Gly Asp Ile Leu Leu Leu Arg Met Gly Gln Arg Asn Leu Val Val
65 70 75 80
Val Ser Ser Pro Asp Leu Ala Lys Glu Val Leu His Thr Gln Gly Val
85 90 95
Glu Phe Gly Ser Arg Thr Arg Asn Val Val Phe Asp Ile Phe Thr Gly
100 105 110
Lys Gly Gln Asp Met Val Phe Thr Val Tyr Gly Glu His Trp Arg Lys
115 120 125
Met Arg Arg Ile Met Thr Val Pro Phe Phe Thr Asn Lys Val Val Gln
130 135 140
Gln Tyr Arg Tyr Gly Trp Glu Asp Glu Ala Ala Arg Val Val Glu Asp
145 150 155 160
Val Lys Lys Asn Pro Glu Ser Ala Thr Asn Gly Ile Val Leu Arg Arg
165 170 175
Arg Leu Gln Leu Met Met Tyr Asn Asn Met Tyr Arg Ile Met Phe Asp
180 185 190
Arg Arg Phe Glu Ser Glu Glu Asp Pro Leu Phe Val Lys Leu Arg Ala
195 200 205
Leu Asn Gly Glu Arg Ser Arg Leu Ala Gln Ser Phe Glu Tyr Asn Tyr
210 215 220
Gly Asp Phe Ile Pro Ile Leu Arg Pro Phe Leu Arg Gly Tyr Leu Lys
225 230 235 240
Ile Cys Lys Glu Val Lys Glu Arg Arg Leu Gln Leu Phe Lys Asp Tyr
245 250 255
Phe Val Asp Glu Arg Lys Lys Leu Gly Ser Thr Lys Ser Met Asn Asn
260 265 270
Glu Gly Leu Lys Cys Ala Ile Asp His Ile Leu Asp Ala Gln Gln Lys
275 280 285
Gly Glu Ile Asn Glu Asp Asn Val Leu Tyr Ile Val Glu Asn Ile Asn
290 295 300
Val Ala Ala Ile Glu Thr Thr Leu Trp Ser Ile Glu Trp Gly Ile Ala
305 310 315 320
Glu Leu Val Asn His Pro Glu Ile Gln Lys Lys Leu Arg Asp Glu Leu
325 330 335
Asp Thr Val Leu Gly Pro Gly Asn Gln Ile Thr Glu Pro Asp Thr Tyr
340 345 350
Lys Leu Pro Tyr Leu Gln Ala Val Val Lys Glu Thr Leu Arg Leu Arg
355 360 365
Met Ala Ile Pro Leu Leu Val Pro His Met Asn Leu His Asp Ala Lys
370 375 380
Leu Ser Gly Tyr Asp Ile Pro Ala Glu Ser Lys Ile Leu Val Asn Ala
385 390 395 400
Trp Trp Leu Ala Asn Asn Pro Ala Asn Trp Lys Asn Pro Glu Glu Phe
405 410 415
Arg Pro Glu Arg Phe Leu Glu Glu Glu Ser Lys Val Glu Ala Asn Gly
420 425 430
Asn Asp Phe Arg Tyr Leu Pro Phe Gly Val Gly Arg Arg Ser Cys Pro
435 440 445
Gly Ile Ile Leu Ala Leu Pro Ile Leu Gly Ile Thr Leu Gly Arg Leu
450 455 460
Val Gln Asn Phe Glu Leu Leu Pro Pro Pro Gly Gln Ser Lys Leu Asp
465 470 475 480
Thr Thr Glu Lys Gly Gly Gln Phe Ser Leu His Ile Leu Lys His Ser
485 490 495
Thr Ile Val Ala Lys Pro Arg Ser Phe
500 505
<210> 134
<211> 506
<212> PRT
<213> 红车轴草()
<220>
<223> 三叶草肉桂酸4-羟化酶 (TpC4H)
<400> 134
Met Asp Leu Leu Leu Leu Glu Lys Thr Leu Leu Ser Leu Phe Ile Ala
1 5 10 15
Ala Ile Ile Ala Ile Thr Ile Ser Lys Leu Arg Gly Lys Arg Phe Lys
20 25 30
Leu Pro Pro Gly Pro Ile Pro Val Pro Ile Phe Gly Asn Trp Leu Gln
35 40 45
Val Gly Asp Asp Leu Asn His Arg Asn Leu Thr Asp Leu Ala Lys Arg
50 55 60
Phe Gly Glu Ile Leu Leu Leu Arg Met Gly Gln Arg Asn Leu Val Val
65 70 75 80
Val Ser Ser Pro Glu Leu Ala Lys Glu Val Leu His Thr Gln Gly Val
85 90 95
Glu Phe Gly Ser Arg Thr Arg Asn Val Val Phe Asp Ile Phe Thr Gly
100 105 110
Lys Gly Gln Asp Met Val Phe Thr Val Tyr Gly Glu His Trp Arg Lys
115 120 125
Met Arg Arg Ile Met Thr Val Pro Phe Phe Thr Asn Lys Val Val Gln
130 135 140
Gln Tyr Arg Phe Gly Trp Glu Ser Glu Ala Glu Ser Val Val Asn Asp
145 150 155 160
Val Lys Lys Asn Ser Asp Ala Ser Val Gly Gly Ile Val Ile Arg Arg
165 170 175
Arg Leu Gln Leu Met Met Tyr Asn Ile Met Tyr Arg Ile Met Phe Asp
180 185 190
Arg Arg Phe Glu Ser Glu Glu Asp Pro Leu Phe Val Lys Leu Lys Ala
195 200 205
Leu Asn Gly Glu Arg Ser Arg Leu Ala Gln Ser Phe Glu Tyr Asn Tyr
210 215 220
Gly Asp Phe Ile Pro Ile Leu Arg Pro Phe Leu Lys Gly Tyr Leu Lys
225 230 235 240
Val Cys Lys Glu Val Lys Asp Arg Arg Leu Gln Leu Phe Lys Asp Tyr
245 250 255
Phe Val Asp Glu Arg Lys Lys Leu Glu Ser Thr Lys Ser Thr Thr Ser
260 265 270
Asn Asp Gly Leu Lys Cys Ala Ile Asp His Ile Leu Asp Ala Gln Lys
275 280 285
Lys Gly Glu Ile Asn Asp Asp Asn Val Leu Tyr Ile Val Glu Asn Ile
290 295 300
Asn Val Ala Ala Ile Glu Thr Thr Leu Trp Ser Ile Glu Trp Gly Ile
305 310 315 320
Ala Glu Leu Val Asn His Gln Glu Ile Gln Asn Lys Val Arg Glu Glu
325 330 335
Met Asp Arg Val Leu Gly Pro Gly His Gln Val Thr Glu Pro Asp Leu
340 345 350
Gln Lys Leu Pro Tyr Leu Gln Ala Val Ile Lys Glu Thr Leu Arg Leu
355 360 365
Arg Met Ala Ile Pro Leu Leu Val Pro His Met Asn Leu His Asp Ala
370 375 380
Lys Leu Ala Gly Tyr Asp Ile Pro Ala Glu Ser Lys Ile Leu Val Asn
385 390 395 400
Ala Trp Trp Leu Ala Asn Asn Pro Ala Leu Trp Lys Lys Pro Glu Glu
405 410 415
Phe Arg Pro Glu Arg Phe Leu Glu Glu Glu Ser His Val Glu Ala Asn
420 425 430
Gly Asn Asp Phe Arg Tyr Leu Pro Phe Gly Val Gly Arg Arg Ser Cys
435 440 445
Pro Gly Ile Ile Leu Ala Leu Pro Ile Leu Gly Ile Thr Ile Gly Arg
450 455 460
Leu Val Gln Asn Phe Glu Leu Leu Pro Pro Pro Gly Gln Ser Lys Ile
465 470 475 480
Asp Thr Ser Glu Lys Gly Gly Gln Phe Ser Leu His Ile Leu Lys His
485 490 495
Ser Thr Val Val Ala Lys Pro Arg Ser Phe
500 505
<210> 135
<211> 531
<212> PRT
<213> 江南卷柏()
<220>
<223> 卷柏肉桂酸4-羟化酶 (SmC4H)
<400> 135
Met Asp Arg Gly Ser Ala Thr Leu Leu Ala Val Ala Ala Met Ile Asn
1 5 10 15
Val Ala Ser Ala Ala Glu Glu Ala Ala Leu Ala Ala Ala Ala Ser Ser
20 25 30
Pro Leu Arg Leu Glu Thr Val Leu Phe Gly Leu Leu Ala Leu Val Leu
35 40 45
Gly Ala Ile Leu Ala Ser Arg Ala Leu Gly Pro Lys Leu Lys Leu Pro
50 55 60
Pro Gly Pro Pro Ala Val Pro Ile Phe Gly Asn Trp Leu Gln Val Gly
65 70 75 80
Asp Asp Leu Asn His Arg Asn Leu Ala Glu Leu Ala Lys Lys Tyr Gly
85 90 95
Glu Ile Phe Leu Leu Lys Met Gly Gln Arg Asn Leu Val Val Val Ser
100 105 110
Ser Pro Glu Leu Ala Lys Glu Val Leu His Thr Gln Gly Val Glu Phe
115 120 125
Gly Ser Arg Thr Arg Asn Val Val Phe Asp Ile Phe Thr Gly Lys Gly
130 135 140
Gln Asp Met Val Phe Thr Val Tyr Gly Glu His Trp Arg Arg Met Arg
145 150 155 160
Arg Ile Met Thr Val Pro Phe Phe Thr Asn Lys Val Val Gln Gln Ser
165 170 175
Arg Pro Val Trp Glu Gln Glu Ile Glu Phe Val Leu Lys Asp Leu Leu
180 185 190
Ala Asn Lys Glu Ala Gln Glu Gly Gly Thr Val Ile Arg Arg Arg Leu
195 200 205
Gln Leu Leu Met Tyr Asn Val Met Tyr Lys Met Met Phe Asp Arg Arg
210 215 220
Phe Glu Ser Glu Asp Asp Pro Leu Phe Leu Lys Leu Arg Gln Leu Asn
225 230 235 240
Gly Glu Arg Ser Arg Leu Ala Gln Ser Phe Glu Tyr Asn Tyr Gly Asp
245 250 255
Phe Ile Pro Ile Leu Arg Pro Phe Leu Lys Arg Tyr Leu Gln Met Cys
260 265 270
Lys Asp Val Lys Glu Asn Arg Leu Gly Leu Phe Lys Lys Tyr Phe Leu
275 280 285
Asp Glu Arg Lys Gln Leu Leu Asn Ala Gly Lys Thr Gly Pro Asp Lys
290 295 300
Val Ala Ile Asp His Ile Leu Glu Ala Gln Lys Gln Gly Glu Ile Thr
305 310 315 320
Glu Ala Asn Val Leu Tyr Ile Val Glu Asn Ile Asn Val Ala Ala Ile
325 330 335
Glu Thr Thr Leu Trp Ser Met Glu Trp Val Ile Ala Glu Leu Val Asn
340 345 350
Asn Arg Asp Ile Gln Asp Lys Val Arg Glu Glu Leu Asp Arg Val Leu
355 360 365
Gly Pro Gly Val Ala Ile Thr Glu Pro Asp Ile Pro Lys Phe Thr Tyr
370 375 380
Leu Thr Ala Val Ile Lys Glu Thr Phe Arg Tyr His Met Ala Ile Pro
385 390 395 400
Leu Leu Val Pro His Met Asn Leu Arg Pro Ala Lys Leu Ala Gly Tyr
405 410 415
Asp Ile Pro Ala Glu Ser Lys Ile Leu Val Asn Ala Trp Trp Leu Gly
420 425 430
Asn Asn Pro Glu Leu Trp Asp Lys Pro Asp Val Phe Asp Pro Ser Arg
435 440 445
Phe Leu Asp Gly Lys Ile Glu Ala Ser Gly Asn Asp Phe Arg Phe Leu
450 455 460
Pro Phe Gly Val Gly Arg Arg Ser Cys Pro Gly Ile Ile Ile Ala Met
465 470 475 480
Pro Leu Leu His Leu Val Ile Gly Ser Leu Val Ala Lys Phe Glu Leu
485 490 495
Leu Pro Pro Pro Gly Cys Asp Lys Ile Asp Val Ser Glu Lys Gly Gly
500 505 510
Gln Phe Ser Leu His Ile Ala Lys His Ser Thr Val Val Val Lys Pro
515 520 525
Arg Val Leu
530
<210> 136
<211> 539
<212> PRT
<213> 人工序列()
<220>
<223> 合成的4-香豆酸-CoA连接酶 (4CL) 共有 序列（大多数）
<220>
<221> 变体
<222> (4)..(.4)
<223> Xaa = Gln, Ser, Glu, Pro, Met, Asp, Asn, Val或Ala
<220>
<221> 变体
<222> (6)..(.6)
<223> Xaa = Ser, Asn, Lys, Glu, Pro, Lys, Ile, Ala或His
<220>
<221> 变体
<222> (51)..(.51)
<223> Xaa = Glu, His, Gln, Arg, Asp, Ala或Thr
<220>
<221> 变体
<222> (52)..(.52)
<223> Xaa = Val, Ile, Thr, Ser, Gln或Glu
<220>
<221> 变体
<222> (61)..(.61)
<223> Xaa = Thr, Ile, Ala, Asn, Leu或Ser
<220>
<221> 变体
<222> (151)..(.151)
<223> Xaa = Thr, Cys, Ala或Ser
<220>
<221> 变体
<222> (174)..(.174)
<223> Xaa = Glu, Val或Ala
<220>
<221> 变体
<222> (202)..(.202)
<223> Xaa = Lys或Arg
<220>
<221> 变体
<222> (291)..(.291)
<223> Xaa = Glu, Ile, Asp, Met, Arg或Ala
<220>
<221> 变体
<222> (310)..(.310)
<223> Xaa = Leu或Met
<220>
<221> 变体
<222> (318)..(.318)
<223> Xaa = Ile, Val, Leu或Phe
<220>
<221> 变体
<222> (322)..(.322)
<223> Xaa = Phe, Leu或Ile
<220>
<221> 变体
<222> (326)..(.326)
<223> Xaa = Lys, Arg, Thr, Ile或Val
<220>
<221> 变体
<222> (357)..(.357)
<223> Xaa = Ala或Ser
<220>
<221> 变体
<222> (437)..(.437)
<223> Xaa = Leu或Ile
<220>
<221> 变体
<222> (456)..(.456)
<223> Xaa = Gly, Asn, Ala, Thr或Ser
<220>
<221> 变体
<222> (509)..(.509)
<223> Xaa = Asn, Lys, Cys或His
<220>
<221> 变体
<222> (520)..(.520)
<223> Xaa = Ala, Ser或Asn
<220>
<221> 变体
<222> (539)..(.539)
<223> Xaa = Met, Pro, Tyr, Val或His
<220>
<221> UNSURE
<222> (4)..(4)
<223> The 'Xaa' at location 4 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (6)..(6)
<223> The 'Xaa' at location 6 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (51)..(51)
<223> The 'Xaa' at location 51 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (52)..(52)
<223> The 'Xaa' at location 52 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (61)..(61)
<223> The 'Xaa' at location 61 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (151)..(151)
<223> The 'Xaa' at location 151 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (174)..(174)
<223> The 'Xaa' at location 174 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (202)..(202)
<223> The 'Xaa' at location 202 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (291)..(291)
<223> The 'Xaa' at location 291 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (310)..(310)
<223> The 'Xaa' at location 310 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (318)..(318)
<223> The 'Xaa' at location 318 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (322)..(322)
<223> The 'Xaa' at location 322 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (326)..(326)
<223> The 'Xaa' at location 326 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (357)..(357)
<223> The 'Xaa' at location 357 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (437)..(437)
<223> The 'Xaa' at location 437 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (456)..(456)
<223> The 'Xaa' at location 456 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (509)..(509)
<223> The 'Xaa' at location 509 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (520)..(520)
<223> The 'Xaa' at location 520 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (539)..(539)
<223> The 'Xaa' at location 539 stands for Gln, Arg, Pro, or Leu.
<400> 136
Glu Ala Lys Xaa Val Xaa Gln Pro Glu Phe Ile Phe Arg Ser Lys Leu
1 5 10 15
Pro Asp Ile Tyr Ile Pro Asn His Leu Pro Leu His Ser Tyr Cys Phe
20 25 30
Glu Asn Ile Ser Glu Phe Ala Asp Arg Pro Cys Leu Ile Asp Gly Ala
35 40 45
Thr Gly Xaa Xaa Tyr Thr Tyr Ala Glu Val Glu Leu Xaa Ser Arg Arg
50 55 60
Val Ala Ala Gly Leu Arg Lys Leu Gly Val Gly Gln Gly Asp Val Val
65 70 75 80
Met Leu Leu Leu Pro Asn Cys Pro Glu Phe Val Phe Ala Phe Leu Gly
85 90 95
Ala Ser Arg Arg Gly Ala Ile Thr Thr Thr Ala Asn Pro Phe Tyr Thr
100 105 110
Pro Ala Glu Ile Ala Lys Gln Ala Lys Ala Ala Gly Ala Lys Leu Ile
115 120 125
Val Thr Gln Ala Cys Tyr Val Glu Lys Val Arg Glu Phe Ala Ala Glu
130 135 140
Arg Gly Val Pro Val Val Xaa Ile Asp Gly Pro Glu Gly Cys Leu His
145 150 155 160
Phe Ser Glu Leu Thr Gln Ala Asp Glu Glu Ala Pro Ala Xaa Asp Ile
165 170 175
His Pro Asp Asp Val Val Ala Leu Pro Tyr Ser Ser Gly Thr Thr Gly
180 185 190
Leu Pro Lys Gly Val Met Leu Thr His Xaa Gly Leu Val Thr Ser Val
195 200 205
Ala Gln Gln Val Asp Gly Glu Asn Pro Asn Leu Tyr Phe His Lys Glu
210 215 220
Asp Val Ile Leu Cys Val Leu Pro Leu Phe His Ile Tyr Ser Leu Asn
225 230 235 240
Ser Val Leu Leu Cys Gly Leu Arg Val Gly Ala Ala Ile Leu Ile Met
245 250 255
Gln Lys Phe Glu Ile Gly Ala Leu Leu Glu Leu Val Gln Arg Tyr Lys
260 265 270
Val Thr Ile Ala Pro Phe Val Pro Pro Ile Val Leu Ala Ile Ala Lys
275 280 285
Ser Pro Xaa Val Asp Ala Tyr Asp Leu Ser Ser Ile Arg Met Val Met
290 295 300
Ser Gly Ala Ala Pro Xaa Gly Lys Glu Leu Glu Asp Ala Xaa Arg Ala
305 310 315 320
Lys Xaa Pro Asn Ala Xaa Leu Gly Gln Gly Tyr Gly Met Thr Glu Ala
325 330 335
Gly Pro Val Leu Ala Met Cys Leu Ala Phe Ala Lys Glu Pro Phe Glu
340 345 350
Val Lys Ser Gly Xaa Cys Gly Thr Val Val Arg Asn Ala Glu Met Lys
355 360 365
Ile Val Asp Pro Asp Thr Gly Ala Ser Leu Pro Arg Asn Gln Pro Gly
370 375 380
Glu Ile Cys Ile Arg Gly Glu Gln Ile Met Lys Gly Tyr Leu Asn Asp
385 390 395 400
Pro Glu Ala Thr Ala Asn Thr Ile Asp Lys Asp Gly Trp Leu His Thr
405 410 415
Gly Asp Ile Gly Tyr Ile Asp Asp Asp Asp Glu Leu Phe Ile Val Asp
420 425 430
Arg Leu Lys Glu Xaa Ile Lys Tyr Lys Gly Phe Gln Val Ala Pro Ala
435 440 445
Glu Leu Glu Ala Leu Leu Ile Xaa His Pro Glu Ile Ser Asp Ala Ala
450 455 460
Val Val Pro Met Lys Asp Glu Leu Ala Gly Glu Val Pro Val Ala Phe
465 470 475 480
Val Val Arg Ser Glu Gly Ser Glu Ile Thr Glu Asp Glu Ile Lys Gln
485 490 495
Phe Val Ser Lys Gln Val Val Phe Tyr Lys Arg Ile Xaa Arg Val Phe
500 505 510
Phe Thr Asp Ser Ile Pro Lys Xaa Pro Ser Gly Lys Ile Leu Arg Lys
515 520 525
Asp Leu Arg Ala Arg Leu Ala Ala Gly Val Xaa
530 535
<210> 137
<211> 539
<212> PRT
<213> 拟南芥()
<220>
<223> 4-香豆酸-CoA连接酶 (At4CL1)
<400> 137
Met Ala Pro Gln Glu Gln Ala Val Ser Gln Val Met Glu Lys Gln Ser
1 5 10 15
Asn Asn Asn Asn Ser Asp Val Ile Phe Arg Ser Lys Leu Pro Asp Ile
20 25 30
Tyr Ile Pro Asn His Leu Ser Leu His Asp Tyr Ile Phe Gln Asn Ile
35 40 45
Ser Glu Phe Ala Thr Lys Pro Cys Leu Ile Asn Gly Pro Thr Gly His
50 55 60
Val Tyr Thr Tyr Ser Asp Val His Val Ile Ser Arg Gln Ile Ala Ala
65 70 75 80
Asn Phe His Lys Leu Gly Val Asn Gln Asn Asp Val Val Met Leu Leu
85 90 95
Leu Pro Asn Cys Pro Glu Phe Val Leu Ser Phe Leu Ala Ala Ser Phe
100 105 110
Arg Gly Ala Thr Ala Thr Ala Ala Asn Pro Phe Phe Thr Pro Ala Glu
115 120 125
Ile Ala Lys Gln Ala Lys Ala Ser Asn Thr Lys Leu Ile Ile Thr Glu
130 135 140
Ala Arg Tyr Val Asp Lys Ile Lys Pro Leu Gln Asn Asp Asp Gly Val
145 150 155 160
Val Ile Val Cys Ile Asp Asp Asn Glu Ser Val Pro Ile Pro Glu Gly
165 170 175
Cys Leu Arg Phe Thr Glu Leu Thr Gln Ser Thr Thr Glu Ala Ser Glu
180 185 190
Val Ile Asp Ser Val Glu Ile Ser Pro Asp Asp Val Val Ala Leu Pro
195 200 205
Tyr Ser Ser Gly Thr Thr Gly Leu Pro Lys Gly Val Met Leu Thr His
210 215 220
Lys Gly Leu Val Thr Ser Val Ala Gln Gln Val Asp Gly Glu Asn Pro
225 230 235 240
Asn Leu Tyr Phe His Ser Asp Asp Val Ile Leu Cys Val Leu Pro Met
245 250 255
Phe His Ile Tyr Ala Leu Asn Ser Ile Met Leu Cys Gly Leu Arg Val
260 265 270
Gly Ala Ala Ile Leu Ile Met Pro Lys Phe Glu Ile Asn Leu Leu Leu
275 280 285
Glu Leu Ile Gln Arg Cys Lys Val Thr Val Ala Pro Met Val Pro Pro
290 295 300
Ile Val Leu Ala Ile Ala Lys Ser Ser Glu Thr Glu Lys Tyr Asp Leu
305 310 315 320
Ser Ser Ile Arg Val Val Lys Ser Gly Ala Ala Pro Leu Gly Lys Glu
325 330 335
Leu Glu Asp Ala Val Asn Ala Lys Phe Pro Asn Ala Lys Leu Gly Gln
340 345 350
Gly Tyr Gly Met Thr Glu Ala Gly Pro Val Leu Ala Met Ser Leu Gly
355 360 365
Phe Ala Lys Glu Pro Phe Pro Val Lys Ser Gly Ala Cys Gly Thr Val
370 375 380
Val Arg Asn Ala Glu Met Lys Ile Val Asp Pro Asp Thr Gly Asp Ser
385 390 395 400
Leu Ser Arg Asn Gln Pro Gly Glu Ile Cys Ile Arg Gly His Gln Ile
405 410 415
Met Lys Gly Tyr Leu Asn Asn Pro Ala Ala Thr Ala Glu Thr Ile Asp
420 425 430
Lys Asp Gly Trp Leu His Thr Gly Asp Ile Gly Leu Ile Asp Asp Asp
435 440 445
Asp Glu Leu Phe Ile Val Asp Arg Leu Lys Glu Leu Ile Lys Tyr Lys
450 455 460
Gly Phe Gln Val Ala Pro Ala Glu Leu Glu Ala Leu Leu Ile Gly His
465 470 475 480
Pro Asp Ile Thr Asp Val Ala Val Val Ala Met Lys Glu Glu Ala Ala
485 490 495
Gly Glu Val Pro Val Ala Phe Val Val Lys Ser Lys Asp Ser Glu Leu
500 505 510
Ser Glu Asp Asp Val Lys Gln Phe Val Ser Lys Gln Val Lys Ser Cys
515 520 525
Val Leu Gln Glu Asn Gln Gln Ser Val Leu His
530 535
<210> 138
<211> 547
<212> PRT
<213> 红花烟草()
<220>
<223> 烟草4-香豆酸-CoA连接酶 (Nt4CL1)
<400> 138
Met Pro Met Glu Thr Thr Thr Glu Thr Lys Gln Ser Gly Asp Leu Ile
1 5 10 15
Phe Arg Ser Lys Leu Pro Asp Ile Tyr Ile Pro Lys His Leu Pro Leu
20 25 30
His Ser Tyr Cys Phe Glu Asn Ile Ser Glu Phe Ser Ser Arg Pro Cys
35 40 45
Leu Ile Asn Gly Ala Asn Asp Gln Ile Tyr Thr Tyr Ala Glu Val Glu
50 55 60
Leu Thr Cys Arg Lys Val Ala Val Gly Leu Asn Lys Leu Gly Ile Gln
65 70 75 80
Gln Lys Asp Thr Ile Met Ile Leu Leu Pro Asn Ser Pro Glu Phe Val
85 90 95
Phe Ala Phe Met Gly Ala Ser Tyr Leu Gly Ala Ile Ser Thr Met Ala
100 105 110
Asn Pro Leu Phe Thr Pro Ala Glu Val Val Lys Gln Ala Lys Ala Ser
115 120 125
Ser Ala Lys Ile Ile Ile Thr Gln Ser Cys Phe Val Gly Lys Val Lys
130 135 140
Asp Tyr Ala Ser Glu Asn Asp Val Lys Val Ile Cys Ile Asp Ser Ala
145 150 155 160
Pro Glu Gly Cys Leu His Phe Ser Glu Leu Thr Gln Ser Asp Glu His
165 170 175
Glu Ile Pro Glu Val Lys Ile Gln Pro Asp Asp Val Val Ala Leu Pro
180 185 190
Tyr Ser Ser Gly Thr Thr Gly Leu Pro Lys Gly Val Met Leu Thr His
195 200 205
Lys Gly Leu Val Thr Ser Val Ala Gln Gln Val Asp Gly Glu Asn Ala
210 215 220
Asn Leu Tyr Met His Ser Glu Asp Val Leu Met Cys Val Leu Pro Leu
225 230 235 240
Phe His Ile Tyr Ser Leu Asn Ser Ile Leu Leu Cys Gly Leu Arg Val
245 250 255
Gly Ala Ala Ile Leu Ile Met Gln Lys Phe Asp Ile Ala Pro Phe Leu
260 265 270
Glu Leu Ile Gln Lys Tyr Lys Val Ser Ile Gly Pro Phe Val Pro Pro
275 280 285
Ile Val Leu Ala Ile Ala Lys Ser Pro Ile Val Asp Ser Tyr Asp Leu
290 295 300
Ser Ser Val Arg Thr Val Met Ser Gly Ala Ala Pro Leu Gly Lys Glu
305 310 315 320
Leu Glu Asp Ala Val Arg Thr Lys Phe Pro Asn Ala Lys Leu Gly Gln
325 330 335
Gly Tyr Gly Met Thr Glu Ala Gly Pro Val Leu Ala Met Cys Leu Ala
340 345 350
Phe Ala Lys Glu Pro Phe Asp Ile Lys Ser Gly Ala Cys Gly Thr Val
355 360 365
Val Arg Asn Ala Glu Met Lys Ile Val Asp Pro Asp Thr Gly Cys Ser
370 375 380
Leu Pro Arg Asn Gln Pro Gly Glu Ile Cys Ile Arg Gly Asp Gln Ile
385 390 395 400
Met Lys Gly Tyr Leu Asn Asp Pro Glu Ala Thr Thr Arg Thr Ile Asp
405 410 415
Lys Glu Gly Trp Leu His Thr Gly Asp Ile Gly Phe Ile Asp Glu Asp
420 425 430
Asp Glu Leu Phe Ile Val Asp Arg Leu Lys Glu Leu Ile Lys Tyr Lys
435 440 445
Gly Phe Gln Val Ala Pro Ala Glu Ile Glu Ala Leu Leu Leu Asn His
450 455 460
Pro Asn Ile Ser Asp Ala Ala Val Val Pro Met Lys Asp Glu Gln Ala
465 470 475 480
Gly Glu Val Pro Val Ala Phe Val Val Arg Ser Asn Gly Ser Ala Ile
485 490 495
Thr Glu Asp Glu Val Lys Asp Phe Ile Ser Lys Gln Val Ile Phe Tyr
500 505 510
Lys Arg Val Lys Arg Val Phe Phe Val Glu Thr Val Pro Lys Ser Pro
515 520 525
Ser Gly Lys Ile Leu Arg Lys Asp Leu Arg Ala Arg Leu Ala Ala Gly
530 535 540
Val Pro Asn
545
<210> 139
<211> 544
<212> PRT
<213> 赤桉()
<220>
<223> 4-香豆酸-CoA连接酶 (Ec4CL)
<400> 139
Met Glu Ala Lys Pro Ser Glu Gln Pro Arg Glu Phe Ile Phe Arg Ser
1 5 10 15
Lys Leu Pro Asp Ile Tyr Ile Pro Asp Asn Leu Ser Leu His Ala Tyr
20 25 30
Cys Phe Glu Asn Ile Ser Glu Phe Ala Asp Arg Pro Cys Val Ile Asn
35 40 45
Gly Ala Thr Gly Arg Thr Tyr Thr Tyr Ala Glu Val Glu Leu Ile Ser
50 55 60
Arg Arg Val Ser Ala Gly Leu Asn Gly Leu Gly Val Gly Gln Gly Asp
65 70 75 80
Val Ile Met Leu Leu Leu Gln Asn Cys Pro Glu Phe Val Phe Ala Phe
85 90 95
Leu Gly Ala Ser Tyr Arg Gly Ala Ile Ser Thr Thr Ala Asn Pro Phe
100 105 110
Tyr Thr Pro Gly Glu Ile Ala Lys Gln Ala Ser Ala Ala Arg Ala Lys
115 120 125
Ile Val Ile Thr Gln Ala Ala Phe Ala Asp Lys Val Arg Pro Phe Ala
130 135 140
Glu Glu Asn Gly Val Lys Val Val Cys Ile Asp Thr Ala Pro Glu Gly
145 150 155 160
Cys Leu His Phe Ser Glu Leu Met Gln Ala Asp Glu Asn Ala Ala Pro
165 170 175
Ala Ala Asp Val Lys Pro Asp Asp Val Leu Ala Leu Pro Tyr Ser Ser
180 185 190
Gly Thr Thr Gly Leu Pro Lys Gly Val Met Leu Thr His Arg Gly Gln
195 200 205
Val Thr Ser Val Ala Gln Gln Val Asp Gly Asp Asn Pro Asn Leu Tyr
210 215 220
Phe His Lys Glu Asp Val Ile Leu Cys Thr Leu Pro Leu Phe His Ile
225 230 235 240
Tyr Ser Leu Asn Ser Val Met Phe Cys Ala Leu Arg Val Gly Ala Ala
245 250 255
Ile Leu Ile Met Gln Lys Phe Glu Ile Val Ala Leu Met Glu Leu Val
260 265 270
Gln Arg Tyr Arg Val Thr Ile Leu Pro Ile Val Pro Pro Ile Val Leu
275 280 285
Glu Ile Ala Lys Ser Ala Glu Val Asp Arg Tyr Asp Leu Ser Ser Ile
290 295 300
Arg Thr Ile Met Ser Gly Ala Ala Pro Met Gly Lys Glu Leu Glu Asp
305 310 315 320
Thr Val Arg Ala Lys Leu Pro Asn Ala Lys Leu Gly Gln Gly Tyr Gly
325 330 335
Met Thr Glu Ala Gly Pro Val Leu Ala Met Cys Pro Ala Phe Ala Lys
340 345 350
Glu Pro Phe Glu Ile Lys Ser Gly Ala Cys Gly Thr Val Val Arg Asn
355 360 365
Ala Glu Met Lys Ile Val Asp Pro Glu Thr Gly Ala Ser Leu Ala Arg
370 375 380
Asn Gln Ala Gly Glu Ile Cys Ile Arg Gly His Gln Ile Met Lys Gly
385 390 395 400
Tyr Leu Asn Asp Ala Glu Ala Thr Ala Asn Thr Ile Asp Lys Glu Gly
405 410 415
Trp Leu His Thr Gly Asp Ile Gly Tyr Ile Asp Asp Asp Asp Glu Leu
420 425 430
Phe Ile Val Asp Arg Leu Lys Glu Leu Ile Lys Tyr Lys Gly Phe Gln
435 440 445
Val Ala Pro Ala Glu Leu Glu Ala Met Leu Ile Ala His Pro Ser Ile
450 455 460
Ser Asp Ala Ala Val Val Pro Met Lys Asp Glu Val Ala Gly Glu Val
465 470 475 480
Pro Val Ala Phe Val Val Lys Ser Asn Gly Ser Val Ile Thr Glu Asp
485 490 495
Glu Ile Lys Gln Tyr Ile Ser Lys Gln Val Val Phe Tyr Lys Arg Ile
500 505 510
Lys Arg Val Phe Phe Thr Asp Ala Ile Pro Lys Ala Pro Ser Gly Lys
515 520 525
Ile Leu Arg Lys Asp Leu Arg Ala Lys Leu Ala Ser Gly Val Tyr Asn
530 535 540
<210> 140
<211> 536
<212> PRT
<213> 火炬松()
<220>
<223> 松树4-香豆酸-CoA连接酶 (Pt4CL1)
<400> 140
Met Asn Pro Gln Glu Glu Phe Ile Phe Arg Ser Lys Leu Pro Asp Ile
1 5 10 15
Tyr Ile Pro Lys Asn Leu Pro Leu His Ser Tyr Val Leu Glu Asn Leu
20 25 30
Ser Asn His Ser Ser Lys Pro Cys Leu Ile Asn Gly Ala Asn Gly Asp
35 40 45
Val Tyr Thr Tyr Ala Asp Val Glu Leu Thr Ala Arg Arg Val Ala Ser
50 55 60
Gly Leu Asn Lys Ile Gly Ile Gln Gln Gly Asp Val Ile Met Leu Phe
65 70 75 80
Leu Pro Ser Ser Pro Glu Phe Val Leu Ala Phe Leu Gly Ala Ser His
85 90 95
Arg Gly Ala Ile Ile Thr Ala Ala Asn Pro Phe Ser Thr Pro Ala Glu
100 105 110
Leu Ala Lys His Ala Lys Ala Ser Arg Ala Lys Leu Leu Ile Thr Gln
115 120 125
Ala Cys Tyr Tyr Glu Lys Val Lys Asp Phe Ala Arg Glu Ser Asp Val
130 135 140
Lys Val Met Cys Val Asp Ser Ala Pro Asp Gly Cys Leu His Phe Ser
145 150 155 160
Glu Leu Thr Gln Ala Asp Glu Asn Glu Ala Pro Gln Val Asp Ile Ser
165 170 175
Pro Asp Asp Val Val Ala Leu Pro Tyr Ser Ser Gly Thr Thr Gly Leu
180 185 190
Pro Lys Gly Val Met Leu Thr His Lys Gly Leu Ile Thr Ser Val Ala
195 200 205
Gln Gln Val Asp Gly Asp Asn Pro Asn Leu Tyr Phe His Ser Glu Asp
210 215 220
Val Ile Leu Cys Val Leu Pro Met Phe His Ile Tyr Ala Leu Asn Ser
225 230 235 240
Ile Met Leu Cys Gly Leu Arg Val Gly Ala Pro Ile Leu Ile Met Pro
245 250 255
Lys Phe Glu Ile Gly Ser Leu Leu Gly Leu Ile Glu Lys Tyr Lys Val
260 265 270
Ser Ile Ala Pro Val Val Pro Pro Val Met Met Ser Ile Ala Lys Ser
275 280 285
Pro Asp Leu Asp Lys His Asp Leu Ser Ser Leu Arg Met Ile Lys Ser
290 295 300
Gly Gly Ala Pro Leu Gly Lys Glu Leu Glu Asp Thr Val Arg Ala Lys
305 310 315 320
Phe Pro Gln Ala Arg Leu Gly Gln Gly Tyr Gly Met Thr Glu Ala Gly
325 330 335
Pro Val Leu Ala Met Cys Leu Ala Phe Ala Lys Glu Pro Phe Asp Ile
340 345 350
Lys Pro Gly Ala Cys Gly Thr Val Val Arg Asn Ala Glu Met Lys Ile
355 360 365
Val Asp Pro Glu Thr Gly Ala Ser Leu Pro Arg Asn Gln Pro Gly Glu
370 375 380
Ile Cys Ile Arg Gly Asp Gln Ile Met Lys Gly Tyr Leu Asn Asp Pro
385 390 395 400
Glu Ala Thr Ser Arg Thr Ile Asp Lys Glu Gly Trp Leu His Thr Gly
405 410 415
Asp Ile Gly Tyr Ile Asp Asp Asp Asp Glu Leu Phe Ile Val Asp Arg
420 425 430
Leu Lys Glu Leu Ile Lys Tyr Lys Gly Phe Gln Val Ala Pro Ala Glu
435 440 445
Leu Glu Ala Leu Leu Ile Ala His Pro Glu Ile Ser Asp Ala Ala Val
450 455 460
Val Gly Leu Lys Asp Glu Asp Ala Gly Glu Val Pro Val Ala Phe Val
465 470 475 480
Val Lys Ser Glu Lys Ser Gln Ala Thr Glu Asp Glu Ile Lys Gln Tyr
485 490 495
Ile Ser Lys Gln Val Ile Phe Tyr Lys Arg Ile Lys Arg Val Phe Phe
500 505 510
Ile Glu Ala Ile Pro Lys Ala Pro Ser Gly Lys Ile Leu Arg Lys Asn
515 520 525
Leu Lys Glu Lys Leu Ala Gly Ile
530 535
<210> 141
<211> 546
<212> PRT
<213> 大豆()
<220>
<223> 大豆4-香豆酸-CoA连接酶 (Gm4CL1)
<400> 141
Met Ala Pro Ser Pro Gln Glu Ile Ile Phe Arg Ser Pro Leu Pro Asp
1 5 10 15
Ile Pro Ile Pro Thr His Leu Pro Leu Tyr Ser Tyr Cys Phe Gln Asn
20 25 30
Leu Ser Gln Phe His Asp Arg Pro Cys Leu Ile Asp Gly Asp Thr Gly
35 40 45
Glu Thr Leu Thr Tyr Ala Asp Val Asp Leu Ala Ala Arg Arg Ile Ala
50 55 60
Ser Gly Leu His Lys Ile Gly Ile Arg Gln Gly Asp Val Ile Met Leu
65 70 75 80
Val Leu Arg Asn Cys Pro Gln Phe Ala Leu Ala Phe Leu Gly Ala Thr
85 90 95
His Arg Gly Ala Val Val Thr Thr Ala Asn Pro Phe Tyr Thr Pro Ala
100 105 110
Glu Leu Ala Lys Gln Ala Thr Ala Thr Lys Thr Arg Leu Val Ile Thr
115 120 125
Gln Ser Ala Tyr Val Glu Lys Ile Lys Ser Phe Ala Asp Ser Ser Ser
130 135 140
Asp Val Met Val Met Cys Ile Asp Asp Asp Phe Ser Tyr Glu Asn Asp
145 150 155 160
Gly Val Leu His Phe Ser Thr Leu Ser Asn Ala Asp Glu Thr Glu Ala
165 170 175
Pro Ala Val Lys Ile Asn Pro Asp Glu Leu Val Ala Leu Pro Phe Ser
180 185 190
Ser Gly Thr Ser Gly Leu Pro Lys Gly Val Met Leu Ser His Lys Asn
195 200 205
Leu Val Thr Thr Ile Ala Gln Leu Val Asp Gly Glu Asn Pro His Gln
210 215 220
Tyr Thr His Ser Glu Asp Val Leu Leu Cys Val Leu Pro Met Phe His
225 230 235 240
Ile Tyr Ala Leu Asn Ser Ile Leu Leu Cys Gly Ile Arg Ser Gly Ala
245 250 255
Ala Val Leu Ile Leu Gln Lys Phe Glu Ile Thr Thr Leu Leu Glu Leu
260 265 270
Ile Glu Lys Tyr Lys Val Thr Val Ala Ser Phe Val Pro Pro Ile Val
275 280 285
Leu Ala Leu Val Lys Ser Gly Glu Thr His Arg Tyr Asp Leu Ser Ser
290 295 300
Ile Arg Ala Val Val Thr Gly Ala Ala Pro Leu Gly Gly Glu Leu Gln
305 310 315 320
Glu Ala Val Lys Ala Arg Leu Pro His Ala Thr Phe Gly Gln Gly Tyr
325 330 335
Gly Met Thr Glu Ala Gly Pro Leu Ala Ile Ser Met Ala Phe Ala Lys
340 345 350
Val Pro Ser Lys Ile Lys Pro Gly Ala Cys Gly Thr Val Val Arg Asn
355 360 365
Ala Glu Met Lys Ile Val Asp Thr Glu Thr Gly Asp Ser Leu Pro Arg
370 375 380
Asn Lys His Gly Glu Ile Cys Ile Ile Gly Thr Lys Val Met Lys Gly
385 390 395 400
Tyr Leu Asn Asp Pro Glu Ala Thr Glu Arg Thr Val Asp Lys Glu Gly
405 410 415
Trp Leu His Thr Gly Asp Ile Gly Phe Ile Asp Asp Asp Asp Glu Leu
420 425 430
Phe Ile Val Asp Arg Leu Lys Glu Leu Ile Lys Tyr Lys Gly Phe Gln
435 440 445
Val Ala Pro Ala Glu Leu Glu Ala Leu Leu Ile Ala His Pro Asn Ile
450 455 460
Ser Asp Ala Ala Val Val Gly Met Lys Asp Glu Ala Ala Gly Glu Ile
465 470 475 480
Pro Val Ala Phe Val Val Arg Ser Asn Gly Ser Glu Ile Ala Glu Asp
485 490 495
Glu Ile Lys Lys Tyr Ile Ser Gln Gln Val Val Phe Tyr Lys Arg Ile
500 505 510
Cys Arg Val Phe Phe Thr Asp Ser Ile Pro Lys Ala Pro Ser Gly Lys
515 520 525
Ile Leu Arg Lys Val Leu Thr Ala Arg Leu Asn Glu Gly Leu Val Val
530 535 540
Ala Asn
545
<210> 142
<211> 544
<212> PRT
<213> 赤桉()
<220>
<223> 4-香豆酸-CoA连接酶 (Ec4CL1)
<400> 142
Met Glu Ala Lys Pro Ser Glu Gln Pro Arg Glu Phe Ile Phe Arg Ser
1 5 10 15
Lys Leu Pro Asp Ile Tyr Ile Pro Asp Asn Leu Ser Leu His Ala Tyr
20 25 30
Cys Phe Glu Asn Ile Ser Glu Phe Ala Asp Arg Pro Cys Val Ile Asn
35 40 45
Gly Ala Thr Gly Arg Thr Tyr Thr Tyr Ala Glu Val Glu Leu Ile Ser
50 55 60
Arg Arg Val Ser Ala Gly Leu Asn Gly Leu Gly Val Gly Gln Gly Asp
65 70 75 80
Val Ile Met Leu Leu Leu Gln Asn Cys Pro Glu Phe Val Phe Ala Phe
85 90 95
Leu Gly Ala Ser Tyr Arg Gly Ala Ile Ser Thr Thr Ala Asn Pro Phe
100 105 110
Tyr Thr Pro Gly Glu Ile Ala Lys Gln Ala Ser Ala Ala Gln Ala Lys
115 120 125
Ile Val Ile Thr Gln Ala Ala Tyr Ala Asp Lys Val Arg Pro Phe Ala
130 135 140
Glu Glu Asn Gly Leu Arg Val Val Cys Ile Asp Thr Ala Pro Glu Gly
145 150 155 160
Cys Leu His Phe Ser Glu Leu Met Gln Ala Asp Glu Asn Ala Ala Pro
165 170 175
Ala Ala Asp Val Lys Pro Asp Asp Val Leu Ala Leu Pro Tyr Ser Ser
180 185 190
Gly Thr Thr Gly Leu Pro Lys Gly Val Met Leu Thr His Arg Gly Gln
195 200 205
Val Thr Ser Val Ala Gln Gln Val Asp Gly Asp Asn Pro Asn Leu Tyr
210 215 220
Phe His Lys Glu Asp Val Ile Leu Cys Thr Leu Pro Leu Phe His Ile
225 230 235 240
Tyr Ser Leu Asn Ser Val Met Phe Cys Ala Leu Arg Val Gly Ala Ala
245 250 255
Ile Leu Ile Met Gln Lys Phe Glu Ile Met Ala Leu Met Glu Leu Val
260 265 270
Gln Arg Tyr Arg Val Thr Ile Leu Pro Ile Val Pro Pro Ile Val Leu
275 280 285
Ala Ile Ala Lys Ser Ala Glu Val Asp Arg Tyr Asp Leu Ser Ser Ile
290 295 300
Arg Thr Ile Met Ser Gly Ala Ala Pro Met Gly Lys Glu Leu Glu Asp
305 310 315 320
Thr Val Arg Ala Lys Leu Pro Asn Ala Lys Leu Gly Gln Gly Tyr Gly
325 330 335
Met Thr Glu Ala Gly Pro Val Leu Ala Met Cys Leu Ala Phe Ala Lys
340 345 350
Glu Pro Phe Glu Ile Lys Ser Gly Ala Cys Gly Thr Val Val Arg Asn
355 360 365
Ala Glu Met Lys Ile Val Asp Pro Glu Thr Gly Ala Ser Leu Pro Arg
370 375 380
Asn Gln Ala Gly Glu Ile Cys Ile Arg Gly His Gln Ile Met Lys Gly
385 390 395 400
Tyr Leu Asn Asp Pro Glu Ala Thr Ala Asn Thr Ile Asp Lys Glu Gly
405 410 415
Trp Leu His Thr Gly Asp Ile Gly Tyr Ile Asp Asp Asp Asp Glu Leu
420 425 430
Phe Ile Val Asp Arg Leu Lys Glu Leu Ile Lys Tyr Lys Gly Phe Gln
435 440 445
Val Ala Pro Ala Glu Leu Glu Ala Met Leu Ile Ala His Pro Ser Ile
450 455 460
Ser Asp Ala Ala Val Val Pro Met Lys Asp Glu Val Ala Ser Glu Val
465 470 475 480
Pro Val Ala Phe Val Val Lys Ser Asn Gly Ser Val Ile Thr Glu Asp
485 490 495
Glu Ile Lys Gln Tyr Ile Ser Lys Gln Val Val Phe Tyr Lys Arg Ile
500 505 510
Asn Arg Val Phe Phe Thr Asp Ala Ile Pro Lys Ala Pro Ser Gly Lys
515 520 525
Ile Leu Arg Lys Asp Leu Arg Ala Lys Leu Ala Ser Gly Val Tyr Asn
530 535 540
<210> 143
<211> 544
<212> PRT
<213> 赤桉()
<220>
<223> 4-香豆酸-CoA连接酶 (Ec4CL2)
<400> 143
Met Glu Ala Lys Pro Ser Glu Gln Pro Arg Glu Phe Ile Phe Arg Ser
1 5 10 15
Lys Leu Pro Asp Ile Tyr Ile Pro Asp Asn Leu Ser Leu His Ala Tyr
20 25 30
Cys Phe Glu Asn Ile Ser Glu Phe Ala Asp Arg Pro Cys Val Ile Asn
35 40 45
Gly Ala Thr Gly Arg Thr Tyr Thr Tyr Ala Glu Val Glu Leu Ile Ser
50 55 60
Arg Arg Val Ser Ala Gly Leu Asn Gly Leu Gly Val Gly Gln Gly Asp
65 70 75 80
Met Ile Met Leu Leu Leu Gln Asn Cys Pro Glu Phe Val Phe Ala Phe
85 90 95
Leu Gly Ala Ser Tyr Arg Gly Ala Ile Ser Thr Thr Ala Asn Pro Phe
100 105 110
Tyr Thr Pro Gly Glu Ile Ala Lys Gln Ala Ser Ala Ala Gln Ala Lys
115 120 125
Ile Val Ile Thr Gln Ala Ala Tyr Ala Asp Lys Val Arg Pro Phe Ala
130 135 140
Glu Glu Asn Gly Leu Ser Val Val Cys Ile Asp Thr Ala Pro Glu Gly
145 150 155 160
Cys Leu His Phe Ser Glu Leu Met Gln Ala Asp Glu Asn Ala Ala Pro
165 170 175
Ala Ala Asp Val Lys Pro Asp Asp Val Leu Ala Leu Pro Tyr Ser Ser
180 185 190
Gly Thr Thr Gly Leu Pro Lys Gly Val Met Leu Thr His Arg Gly Gln
195 200 205
Val Thr Ser Val Ala Gln Gln Val Asp Gly Asp Asn Pro Asn Leu Tyr
210 215 220
Phe His Lys Glu Asp Val Ile Leu Cys Thr Leu Pro Leu Phe His Ile
225 230 235 240
Tyr Ser Leu Asn Ser Val Met Phe Cys Ala Leu Arg Val Gly Ala Ala
245 250 255
Ile Leu Ile Met Gln Lys Phe Glu Ile Met Ala Leu Met Glu Leu Val
260 265 270
Gln Arg Tyr Arg Val Thr Ile Leu Pro Ile Val Pro Pro Ile Val Leu
275 280 285
Ala Ile Ala Lys Ser Ala Glu Val Asp Arg Tyr Asp Leu Ser Ser Ile
290 295 300
Arg Thr Ile Met Ser Gly Ala Ala Pro Met Gly Lys Glu Leu Glu Asp
305 310 315 320
Thr Val Arg Ala Lys Leu Pro Asn Ala Lys Leu Gly Gln Gly Tyr Gly
325 330 335
Met Thr Glu Ala Gly Pro Val Leu Ala Met Cys Leu Ala Phe Ala Lys
340 345 350
Glu Pro Phe Glu Ile Lys Ser Gly Ala Cys Gly Thr Val Val Arg Asn
355 360 365
Ala Glu Met Lys Ile Val Asp Pro Glu Thr Gly Ala Ser Leu Pro Arg
370 375 380
Asn Gln Ala Gly Glu Ile Cys Ile Arg Gly His Gln Ile Met Lys Gly
385 390 395 400
Tyr Leu Asn Asp Pro Glu Ala Thr Ala Asn Thr Ile Asp Lys Glu Gly
405 410 415
Trp Leu His Thr Gly Asp Ile Gly Tyr Ile Asp Asp Asp Asp Glu Leu
420 425 430
Phe Ile Val Asp Arg Leu Lys Glu Leu Ile Lys Tyr Lys Gly Phe Gln
435 440 445
Val Ala Pro Ala Glu Leu Glu Ala Met Leu Ile Ala His Pro Ser Ile
450 455 460
Ser Asp Ala Ala Val Val Pro Met Lys Asp Glu Val Ala Ser Glu Val
465 470 475 480
Pro Val Ala Phe Val Val Lys Ser Asn Gly Ser Val Ile Thr Glu Asp
485 490 495
Glu Ile Lys Gln Tyr Ile Ser Lys Gln Val Val Phe Tyr Lys Arg Ile
500 505 510
Asn Arg Val Phe Phe Thr Asp Ala Ile Pro Lys Ala Pro Ser Gly Lys
515 520 525
Ile Leu Arg Lys Asp Leu Arg Ala Lys Leu Ala Ser Gly Val Tyr Asn
530 535 540
<210> 144
<211> 462
<212> PRT
<213> 红花烟草()
<220>
<223> 烟草4-香豆酸-CoA连接酶 (Nt4CL2)
<400> 144
Met Glu Lys Asp Thr Lys Gln Val Asp Ile Ile Phe Arg Ser Lys Leu
1 5 10 15
Pro Asp Ile Tyr Ile Pro Asn His Leu Pro Leu His Ser Tyr Cys Phe
20 25 30
Glu Asn Ile Ser Glu Phe Ser Ser Arg Pro Cys Leu Ile Asn Gly Ala
35 40 45
Asn Lys Gln Ile Tyr Thr Tyr Ala Asp Val Glu Leu Asn Ser Arg Lys
50 55 60
Val Ala Ala Gly Leu His Lys Gln Gly Ile Gln Pro Lys Asp Thr Ile
65 70 75 80
Met Ile Leu Leu Pro Asn Ser Pro Glu Phe Val Phe Ala Phe Ile Gly
85 90 95
Ala Ser Tyr Leu Gly Ala Ile Ser Thr Met Ala Asn Pro Leu Phe Thr
100 105 110
Pro Ala Glu Val Val Lys Gln Ala Lys Ala Ser Ser Ala Lys Ile Ile
115 120 125
Val Thr Gln Ala Cys His Val Asn Lys Val Lys Asp Tyr Ala Phe Glu
130 135 140
Asn Asp Val Lys Ile Ile Cys Ile Asp Ser Ala Pro Glu Gly Cys Leu
145 150 155 160
His Phe Ser Val Leu Thr Gln Ala Asn Glu His Asp Ile Pro Glu Val
165 170 175
Glu Ile Gln Pro Asp Asp Val Val Ala Leu Pro Tyr Ser Ser Gly Thr
180 185 190
Thr Gly Leu Pro Lys Gly Val Met Leu Thr His Lys Gly Leu Val Thr
195 200 205
Ser Val Ala Gln Gln Val Asp Gly Glu Asn Pro Asn Leu Tyr Ile His
210 215 220
Ser Glu Asp Val Met Leu Cys Val Leu Pro Leu Phe His Ile Tyr Ser
225 230 235 240
Leu Asn Ser Val Leu Leu Cys Gly Leu Arg Val Gly Ala Ala Ile Leu
245 250 255
Ile Met Gln Lys Phe Asp Ile Val Ser Phe Leu Glu Leu Ile Gln Arg
260 265 270
Tyr Lys Val Thr Ile Gly Pro Phe Val Pro Pro Ile Val Leu Ala Ile
275 280 285
Ala Lys Ser Pro Met Val Asp Asp Tyr Asp Leu Ser Ser Val Arg Thr
290 295 300
Val Met Ser Gly Ala Ala Pro Leu Gly Lys Glu Leu Glu Asp Thr Val
305 310 315 320
Arg Ala Lys Phe Pro Asn Ala Lys Leu Gly Gln Gly Tyr Gly Met Thr
325 330 335
Glu Ala Gly Pro Val Leu Ala Met Cys Leu Ala Phe Ala Lys Glu Pro
340 345 350
Phe Glu Ile Lys Ser Gly Ala Cys Gly Thr Val Val Arg Asn Ala Glu
355 360 365
Met Lys Ile Val Asp Pro Lys Thr Gly Asn Ser Leu Pro Arg Asn Gln
370 375 380
Ser Gly Glu Ile Cys Ile Arg Gly Asp Gln Ile Met Lys Gly Tyr Leu
385 390 395 400
Asn Asp Pro Glu Ala Thr Asn Gly Ser Thr Ile Thr Glu Asp Glu Val
405 410 415
Lys Asp Phe Ile Ser Lys Gln Val Ile Phe Tyr Lys Arg Ile Lys Arg
420 425 430
Val Phe Phe Val Asp Ala Ile Pro Lys Ser Pro Ser Gly Lys Ile Leu
435 440 445
Arg Lys Asp Leu Arg Ala Lys Leu Ala Ala Gly Leu Pro Asn
450 455 460
<210> 145
<211> 537
<212> PRT
<213> 火炬松()
<220>
<223> 松树4-香豆酸-CoA连接酶 (Pt4CL)
<400> 145
Met Ala Asn Gly Ile Lys Lys Val Glu His Leu Tyr Arg Ser Lys Leu
1 5 10 15
Pro Asp Ile Glu Ile Ser Asp His Leu Pro Leu His Ser Tyr Cys Phe
20 25 30
Glu Arg Val Ala Glu Phe Ala Asp Arg Pro Cys Leu Ile Asp Gly Ala
35 40 45
Thr Asp Arg Thr Tyr Cys Phe Ser Glu Val Glu Leu Ile Ser Arg Lys
50 55 60
Val Ala Ala Gly Leu Ala Lys Leu Gly Leu Gln Gln Gly Gln Val Val
65 70 75 80
Met Leu Leu Leu Pro Asn Cys Ile Glu Phe Ala Phe Val Phe Met Gly
85 90 95
Ala Ser Val Arg Gly Ala Ile Val Thr Thr Ala Asn Pro Phe Tyr Lys
100 105 110
Pro Gly Glu Ile Ala Lys Gln Ala Lys Ala Ala Gly Ala Arg Ile Ile
115 120 125
Val Thr Leu Ala Ala Tyr Val Glu Lys Leu Ala Asp Leu Gln Ser His
130 135 140
Asp Val Leu Val Ile Thr Ile Asp Asp Ala Pro Lys Glu Gly Cys Gln
145 150 155 160
His Ile Ser Val Leu Thr Glu Ala Asp Glu Thr Gln Cys Pro Ala Val
165 170 175
Lys Ile His Pro Asp Asp Val Val Ala Leu Pro Tyr Ser Ser Gly Thr
180 185 190
Thr Gly Leu Pro Lys Gly Val Met Leu Thr His Lys Gly Leu Val Ser
195 200 205
Ser Val Ala Gln Gln Val Asp Gly Glu Asn Pro Asn Leu Tyr Phe His
210 215 220
Ser Asp Asp Val Ile Leu Cys Val Leu Pro Leu Phe His Ile Tyr Ser
225 230 235 240
Leu Asn Ser Val Leu Leu Cys Ala Leu Arg Ala Gly Ala Ala Thr Leu
245 250 255
Ile Met Gln Lys Phe Asn Leu Thr Thr Cys Leu Glu Leu Ile Gln Lys
260 265 270
Tyr Lys Val Thr Val Ala Pro Ile Val Pro Pro Ile Val Leu Asp Ile
275 280 285
Thr Lys Ser Pro Ile Val Ser Gln Tyr Asp Val Ser Ser Val Arg Ile
290 295 300
Ile Met Ser Gly Ala Ala Pro Leu Gly Lys Glu Leu Glu Asp Ala Leu
305 310 315 320
Arg Glu Arg Phe Pro Lys Ala Ile Phe Gly Gln Gly Tyr Gly Met Thr
325 330 335
Glu Ala Gly Pro Val Leu Ala Met Asn Leu Ala Phe Ala Lys Asn Pro
340 345 350
Phe Pro Val Lys Ser Gly Ser Cys Gly Thr Val Val Arg Asn Ala Gln
355 360 365
Ile Lys Ile Leu Asp Thr Glu Thr Gly Glu Ser Leu Pro His Asn Gln
370 375 380
Ala Gly Glu Ile Cys Ile Arg Gly Pro Glu Ile Met Lys Gly Tyr Ile
385 390 395 400
Asn Asp Pro Glu Ser Thr Ala Ala Thr Ile Asp Glu Glu Gly Trp Leu
405 410 415
His Thr Gly Asp Val Gly Tyr Ile Asp Asp Asp Glu Glu Ile Phe Ile
420 425 430
Val Asp Arg Val Lys Glu Ile Ile Lys Tyr Lys Gly Phe Gln Val Ala
435 440 445
Pro Ala Glu Leu Glu Ala Leu Leu Val Ala His Pro Ser Ile Ala Asp
450 455 460
Ala Ala Val Val Pro Gln Lys His Glu Glu Ala Gly Glu Val Pro Val
465 470 475 480
Ala Phe Val Val Lys Ser Ser Glu Ile Ser Glu Gln Glu Ile Lys Glu
485 490 495
Phe Val Ala Lys Gln Val Ile Phe Tyr Lys Lys Ile His Arg Val Tyr
500 505 510
Phe Val Asp Ala Ile Pro Lys Ser Pro Ser Gly Lys Ile Leu Arg Lys
515 520 525
Asp Leu Arg Ser Arg Leu Ala Ala Lys
530 535
<210> 146
<211> 554
<212> PRT
<213> 水稻()
<220>
<223> 稻4-香豆酸-CoA连接酶 (Os4CL3)
<400> 146
Met Gly Ser Val Ala Ala Glu Glu Val Val Val Phe Arg Ser Lys Leu
1 5 10 15
Pro Asp Ile Glu Ile Asp Asn Ser Met Thr Leu Gln Glu Tyr Cys Phe
20 25 30
Ala Arg Met Ala Glu Val Gly Ala Arg Pro Cys Leu Ile Asp Gly Gln
35 40 45
Thr Gly Glu Ser Tyr Thr Tyr Ala Glu Val Glu Ser Ala Ser Arg Arg
50 55 60
Ala Ala Ala Gly Leu Arg Arg Met Gly Val Gly Lys Gly Asp Val Val
65 70 75 80
Met Ser Leu Leu Arg Asn Cys Pro Glu Phe Ala Phe Ser Phe Leu Gly
85 90 95
Ala Ala Arg Leu Gly Ala Ala Thr Thr Thr Ala Asn Pro Phe Tyr Thr
100 105 110
Pro His Glu Val His Arg Gln Ala Glu Ala Ala Gly Ala Arg Val Ile
115 120 125
Val Thr Glu Ala Cys Ala Val Glu Lys Val Arg Glu Phe Ala Ala Glu
130 135 140
Arg Gly Val Pro Val Val Thr Val Asp Gly Ala Phe Asp Gly Cys Val
145 150 155 160
Glu Phe Arg Glu Val Leu Ala Ala Glu Glu Leu Asp Ala Asp Ala Asp
165 170 175
Val His Pro Asp Asp Val Val Ala Leu Pro Tyr Ser Ser Gly Thr Thr
180 185 190
Gly Leu Pro Lys Gly Val Met Leu Thr His Arg Ser Leu Ile Thr Ser
195 200 205
Val Ala Gln Gln Val Asp Gly Glu Asn Pro Asn Leu Tyr Phe Ser Lys
210 215 220
Asp Asp Val Ile Leu Cys Leu Leu Pro Leu Phe His Ile Tyr Ser Leu
225 230 235 240
Asn Ser Val Leu Leu Ala Gly Leu Arg Ala Gly Ser Thr Ile Val Ile
245 250 255
Met Arg Lys Phe Asp Leu Gly Ala Leu Val Asp Leu Val Arg Lys His
260 265 270
Asn Ile Thr Ile Ala Pro Phe Val Pro Pro Ile Val Val Glu Ile Ala
275 280 285
Lys Ser Pro Arg Val Thr Ala Glu Asp Leu Ala Ser Ile Arg Met Val
290 295 300
Met Ser Gly Ala Ala Pro Met Gly Lys Asp Leu Gln Asp Ala Phe Met
305 310 315 320
Ala Lys Ile Pro Asn Ala Val Leu Gly Gln Gly Tyr Gly Met Thr Glu
325 330 335
Ala Gly Pro Val Leu Ala Met Cys Leu Ala Phe Ala Lys Glu Pro Phe
340 345 350
Lys Val Lys Ser Gly Ser Cys Gly Thr Val Val Arg Asn Ala Glu Leu
355 360 365
Lys Ile Val Asp Pro Asp Thr Gly Thr Ser Leu Gly Arg Asn Gln Ser
370 375 380
Gly Glu Ile Cys Ile Arg Gly Glu Gln Ile Met Lys Gly Tyr Leu Asn
385 390 395 400
Asp Pro Glu Ala Thr Lys Asn Thr Ile Asp Glu Asp Gly Trp Leu His
405 410 415
Thr Gly Asp Ile Gly Phe Val Asp Asp Asp Asp Glu Ile Phe Ile Val
420 425 430
Asp Arg Leu Lys Glu Ile Ile Lys Tyr Lys Gly Phe Gln Val Pro Pro
435 440 445
Ala Glu Leu Glu Ala Leu Leu Ile Thr His Pro Glu Ile Lys Asp Ala
450 455 460
Ala Val Val Ser Met Lys Asp Asp Leu Ala Gly Glu Val Pro Val Ala
465 470 475 480
Phe Ile Val Arg Thr Glu Gly Ser Glu Ile Thr Glu Asp Glu Ile Lys
485 490 495
Lys Phe Val Ala Lys Glu Val Val Phe Tyr Lys Arg Ile Asn Lys Val
500 505 510
Phe Phe Thr Asp Ser Ile Pro Lys Asn Pro Ser Gly Lys Ile Leu Arg
515 520 525
Lys Asp Leu Arg Ala Arg Leu Ala Ala Gly Ile Pro Asp Ala Val Ala
530 535 540
Ala Ala Ala Ala Asp Ala Pro Lys Ser Ser
545 550
<210> 147
<211> 555
<212> PRT
<213> 两色高梁()
<220>
<223> 高粱4-香豆酸-CoA连接酶 (Sb4CL)
<400> 147
Met Gly Ser Val Asp Thr Ala Val Ala Val Pro Val Pro Val Pro Glu
1 5 10 15
Pro Glu Ala Glu Glu Lys Ala Ala Val Val Phe Arg Ser Lys Leu Pro
20 25 30
Asp Ile Glu Ile Asn Asn Ser Gln Ser Leu Gln Thr Tyr Cys Phe Gly
35 40 45
Lys Met Ser Glu Val Ala Asp Arg Ala Cys Leu Ile Asp Gly Gln Thr
50 55 60
Gly Ala Ser Tyr Thr Tyr Ala Glu Val Glu Ser Leu Ser Arg Arg Ala
65 70 75 80
Ala Ser Gly Leu Arg Ala Met Gly Val Gly Lys Gly Asp Val Val Met
85 90 95
Asn Leu Leu Arg Asn Cys Pro Glu Phe Ala Phe Thr Phe Leu Gly Ala
100 105 110
Ala Arg Leu Gly Ala Ala Thr Thr Thr Ala Asn Pro Phe Tyr Thr Pro
115 120 125
His Glu Ile His Arg Gln Ala Glu Ala Ala Gly Ala Lys Val Ile Val
130 135 140
Thr Glu Ala Cys Ala Val Glu Lys Val Arg Glu Phe Ala Ala Gly Arg
145 150 155 160
Gly Val Pro Val Val Thr Val Asp Gly Arg Phe Asp Gly Cys Val Glu
165 170 175
Phe Ala Glu Val Ile Ala Ala Glu Glu Leu Asp Ala Asp Ala Asp Val
180 185 190
His Pro Asp Asp Val Val Ala Leu Pro Tyr Ser Ser Gly Thr Thr Gly
195 200 205
Leu Pro Lys Gly Val Met Leu Thr His Arg Ser Leu Ile Thr Ser Val
210 215 220
Ala Gln Gln Val Asp Gly Glu Asn Pro Asn Leu Tyr Phe Ser Lys Asp
225 230 235 240
Asp Val Val Leu Cys Leu Leu Pro Leu Phe His Ile Tyr Ser Leu Asn
245 250 255
Ser Val Leu Leu Ala Gly Leu Arg Ala Gly Ser Thr Ile Val Ile Met
260 265 270
Arg Lys Phe Asp Leu Gly Ala Leu Val Asp Leu Val Arg Lys His Gly
275 280 285
Ile Thr Ile Ala Pro Phe Val Pro Pro Ile Val Val Glu Ile Ala Lys
290 295 300
Ser Pro Arg Val Thr Ala Asp Asp Leu Ala Ser Ile Arg Met Val Met
305 310 315 320
Ser Gly Ala Ala Pro Met Gly Lys Glu Leu Gln Asp Ala Phe Met Thr
325 330 335
Lys Ile Pro Asn Ala Val Leu Gly Gln Gly Tyr Gly Met Thr Glu Ala
340 345 350
Gly Pro Val Leu Ala Met Cys Leu Ala Phe Ala Lys Glu Pro Phe Gln
355 360 365
Val Lys Ser Gly Ser Cys Gly Thr Val Val Arg Asn Ala Glu Leu Lys
370 375 380
Val Val Asp Pro Asp Thr Gly Ala Ala Leu Gly Arg Asn Gln Pro Gly
385 390 395 400
Glu Ile Cys Ile Arg Gly Glu Gln Ile Met Lys Gly Tyr Leu Asn Asp
405 410 415
Leu Glu Ser Thr Lys Asn Thr Ile Asp Lys Asp Gly Trp Leu His Thr
420 425 430
Gly Asp Ile Gly Tyr Val Asp Asp Asp Asp Glu Ile Phe Ile Val Asp
435 440 445
Arg Leu Lys Glu Ile Ile Lys Tyr Lys Gly Phe Gln Val Pro Pro Ala
450 455 460
Glu Leu Glu Ala Leu Leu Ile Thr His Pro Glu Ile Lys Asp Ala Ala
465 470 475 480
Val Val Ser Met Lys Asp Asp Leu Ala Gly Glu Ile Pro Val Ala Phe
485 490 495
Ile Val Arg Thr Glu Gly Ser Glu Val Thr Glu Asp Glu Ile Lys Gln
500 505 510
Phe Val Ala Lys Glu Val Val Phe Tyr Lys Lys Ile His Lys Val Phe
515 520 525
Phe Thr Glu Ser Ile Pro Lys Asn Pro Ser Gly Lys Ile Leu Arg Lys
530 535 540
Asp Leu Arg Ala Arg Leu Ala Ala Gly Val His
545 550 555
<210> 148
<211> 555
<212> PRT
<213> 玉米()
<220>
<223> 玉米4-香豆酸-CoA连接酶 (Zm4CL)
<400> 148
Met Gly Ser Val Asp Ala Ala Ile Ala Val Pro Val Pro Ala Ala Glu
1 5 10 15
Glu Lys Ala Val Glu Glu Lys Ala Met Val Phe Arg Ser Lys Leu Pro
20 25 30
Asp Ile Glu Ile Asp Ser Ser Met Ala Leu His Thr Tyr Cys Phe Gly
35 40 45
Lys Met Gly Glu Val Ala Glu Arg Ala Cys Leu Ile Asp Gly Leu Thr
50 55 60
Gly Ala Ser Tyr Thr Tyr Ala Glu Val Glu Ser Leu Ser Arg Arg Ala
65 70 75 80
Ala Ser Gly Leu Arg Ala Met Gly Val Gly Lys Gly Asp Val Val Met
85 90 95
Ser Leu Leu Arg Asn Cys Pro Glu Phe Ala Phe Thr Phe Leu Gly Ala
100 105 110
Ala Arg Leu Gly Ala Ala Thr Thr Thr Ala Asn Pro Phe Tyr Thr Pro
115 120 125
His Glu Val His Arg Gln Ala Glu Ala Ala Gly Ala Arg Leu Ile Val
130 135 140
Thr Glu Ala Cys Ala Val Glu Lys Val Arg Glu Phe Ala Ala Glu Arg
145 150 155 160
Gly Ile Pro Val Val Thr Val Asp Gly Arg Phe Asp Gly Cys Val Glu
165 170 175
Phe Ala Glu Leu Ile Ala Ala Glu Glu Leu Glu Ala Asp Ala Asp Ile
180 185 190
His Pro Asp Asp Val Val Ala Leu Pro Tyr Ser Ser Gly Thr Thr Gly
195 200 205
Leu Pro Lys Gly Val Met Leu Thr His Arg Ser Leu Ile Thr Ser Val
210 215 220
Ala Gln Gln Val Asp Gly Glu Asn Pro Asn Leu Tyr Phe Arg Lys Asp
225 230 235 240
Asp Val Val Leu Cys Leu Leu Pro Leu Phe His Ile Tyr Ser Leu Asn
245 250 255
Ser Val Leu Leu Ala Gly Leu Arg Ala Gly Ser Thr Ile Val Ile Met
260 265 270
Arg Lys Phe Asp Leu Gly Ala Leu Val Asp Leu Val Arg Arg Tyr Val
275 280 285
Ile Thr Ile Ala Pro Phe Val Pro Pro Ile Val Val Glu Ile Ala Lys
290 295 300
Ser Pro Arg Val Thr Ala Gly Asp Leu Ala Ser Ile Arg Met Val Met
305 310 315 320
Ser Gly Ala Ala Pro Met Gly Lys Glu Leu Gln Asp Ala Phe Met Ala
325 330 335
Lys Ile Pro Asn Ala Val Leu Gly Gln Gly Tyr Gly Met Thr Glu Ala
340 345 350
Gly Pro Val Leu Ala Met Cys Leu Ala Phe Ala Lys Glu Pro Tyr Pro
355 360 365
Val Lys Ser Gly Ser Cys Gly Thr Val Val Arg Asn Ala Glu Leu Lys
370 375 380
Ile Val Asp Pro Asp Thr Gly Ala Ala Leu Gly Arg Asn Gln Pro Gly
385 390 395 400
Glu Ile Cys Ile Arg Gly Glu Gln Ile Met Lys Gly Tyr Leu Asn Asp
405 410 415
Pro Glu Ser Thr Lys Asn Thr Ile Asp Gln Asp Gly Trp Leu His Thr
420 425 430
Gly Asp Ile Gly Tyr Val Asp Asp Asp Asp Glu Ile Phe Ile Val Asp
435 440 445
Arg Leu Lys Glu Ile Ile Lys Tyr Lys Gly Phe Gln Val Pro Pro Ala
450 455 460
Glu Leu Glu Ala Leu Leu Ile Thr His Pro Glu Ile Lys Asp Ala Ala
465 470 475 480
Val Val Ser Met Asn Asp Asp Leu Ala Gly Glu Ile Pro Val Ala Phe
485 490 495
Ile Val Arg Thr Glu Gly Ser Gln Val Thr Glu Asp Glu Ile Lys Gln
500 505 510
Phe Val Ala Lys Glu Val Val Phe Tyr Lys Lys Ile His Lys Val Phe
515 520 525
Phe Thr Glu Ser Ile Pro Lys Asn Pro Ser Gly Lys Ile Leu Arg Lys
530 535 540
Asp Leu Arg Ala Arg Leu Ala Ala Gly Val His
545 550 555
<210> 149
<211> 542
<212> PRT
<213> 柳枝稷()
<220>
<223> 柳枝稷4-香豆酸-CoA连接酶 (Pv4CL)
<400> 149
Met Gly Ser Val Asp Glu Ser Ala Ala Ala Glu Val Val Phe Arg Ser
1 5 10 15
Lys Leu Pro Asp Ile Glu Ile Asn Asn Ser Gln Pro Leu His Ala Tyr
20 25 30
Cys Phe Gly Lys Met Pro Glu Val Ala Gly Arg Pro Cys Leu Ile Asp
35 40 45
Gly Gln Thr Gly Ala Ser Tyr Thr Tyr Ala Glu Val Glu Ser Leu Ser
50 55 60
Arg Arg Ala Ala Ala Gly Leu Arg Arg Met Gly Val Gly Lys Gly Asp
65 70 75 80
Val Val Met Ser Leu Leu Arg Asn Cys Pro Glu Phe Ala Phe Ala Phe
85 90 95
Leu Gly Ala Ala Arg Leu Gly Ala Ala Thr Thr Thr Ala Asn Pro Phe
100 105 110
Tyr Thr Pro His Glu Ile His Arg Gln Ala Glu Ala Ala Gly Ala Lys
115 120 125
Leu Ile Val Thr Glu Ala Cys Ala Val Asp Lys Val Arg Glu Phe Ala
130 135 140
Val Ala Arg Gly Ile Pro Val Val Thr Val Asp Gly Arg Phe Asp Gly
145 150 155 160
Cys Ala Glu Phe Ala Glu Val Ile Ala Ala Glu Glu Leu Glu Ala Asp
165 170 175
Ala Asp Ile Gln Pro Asp Asp Val Val Ala Leu Pro Tyr Ser Ser Gly
180 185 190
Thr Thr Gly Leu Pro Lys Gly Val Met Leu Thr His Arg Ser Leu Ile
195 200 205
Thr Ser Val Ala Gln Gln Val Asp Gly Glu Asn Pro Asn Leu His Phe
210 215 220
Arg Lys Asp Asp Val Leu Leu Cys Leu Leu Pro Leu Phe His Ile Tyr
225 230 235 240
Ser Leu Asn Ser Val Leu Leu Ala Gly Leu Arg Ala Gly Cys Ala Ile
245 250 255
Val Ile Met Arg Lys Phe Glu Thr Gly Ala Leu Val Glu Leu Val Arg
260 265 270
Ala His Gly Val Thr Val Ala Pro Phe Val Pro Pro Ile Val Val Glu
275 280 285
Ile Ala Lys Ser Pro Arg Val Gly Ala Ala Asp Leu Ala Ser Ile Arg
290 295 300
Met Val Met Ser Gly Ala Ala Pro Met Gly Lys Asp Leu Gln Asp Ala
305 310 315 320
Phe Met Ala Lys Ile Pro Asn Ala Val Leu Gly Gln Gly Tyr Gly Met
325 330 335
Thr Glu Ala Gly Pro Val Leu Ala Met Cys Leu Ala Phe Ala Lys Glu
340 345 350
Pro Phe Gln Val Lys Ser Gly Ser Cys Gly Thr Val Val Arg Asn Ala
355 360 365
Glu Leu Lys Ile Val Asp Pro Asp Thr Gly Ala Ala Leu Gly Arg Asn
370 375 380
Gln Pro Gly Glu Ile Cys Ile Arg Gly Glu Gln Ile Met Lys Gly Tyr
385 390 395 400
Leu Asn Asp Pro Glu Ser Thr Lys Asn Thr Ile Asp Lys Asp Gly Trp
405 410 415
Leu His Thr Gly Asp Ile Gly Tyr Val Asp Asp Asp Asp Glu Ile Phe
420 425 430
Ile Val Asp Arg Leu Lys Glu Ile Ile Lys Tyr Lys Gly Phe Gln Val
435 440 445
Pro Pro Ala Glu Leu Glu Ala Leu Leu Ile Thr His Pro Glu Ile Lys
450 455 460
Asp Ala Ala Val Val Ser Met Lys Asp Asp Leu Ala Gly Glu Ile Pro
465 470 475 480
Val Ala Phe Ile Val Arg Thr Glu Gly Ser Gln Leu Thr Glu Asp Glu
485 490 495
Ile Lys Gln Phe Val Ala Lys Glu Val Val Phe Tyr Lys Lys Ile His
500 505 510
Lys Val Phe Phe Thr Asp Ser Ile Pro Lys Asn Pro Ser Gly Lys Ile
515 520 525
Leu Arg Lys Asp Leu Arg Ala Arg Leu Ala Ala Gly Val His
530 535 540
<210> 150
<211> 559
<212> PRT
<213> 水稻()
<220>
<223> 稻4-香豆酸-CoA连接酶 (Os4CL4)
<400> 150
Met Gly Ser Met Ala Ala Ala Ala Glu Ala Ala Gln Glu Glu Glu Thr
1 5 10 15
Val Val Phe Arg Ser Lys Leu Pro Asp Ile Glu Ile Pro Ser His Leu
20 25 30
Thr Leu Gln Ala Tyr Cys Phe Glu Lys Leu Pro Glu Val Ala Ala Arg
35 40 45
Pro Cys Leu Ile Asp Gly Gln Thr Gly Ala Val Tyr Ser Tyr Gly Glu
50 55 60
Val Glu Glu Leu Ser Arg Arg Ala Ala Ala Gly Leu Arg Arg Leu Gly
65 70 75 80
Val Gly Lys Gly Asp Val Val Met Ser Leu Leu Arg Asn Cys Pro Glu
85 90 95
Phe Ala Phe Thr Phe Leu Gly Ala Ala Arg Leu Gly Ala Ala Thr Thr
100 105 110
Thr Ala Asn Pro Phe Tyr Thr Pro His Glu Ile His Arg Gln Ala Ser
115 120 125
Ala Ala Gly Ala Arg Val Ile Val Thr Glu Ala Cys Ala Val Glu Lys
130 135 140
Val Arg Gly Phe Ala Ala Asp Arg Gly Ile Pro Val Val Ala Val Asp
145 150 155 160
Gly Asp Phe Asp Gly Cys Val Gly Phe Gly Glu Ala Met Leu Asp Ala
165 170 175
Ser Ile Glu Pro Leu Asp Ala Asp Glu Glu Val His Pro Asp Asp Val
180 185 190
Val Ala Leu Pro Tyr Ser Ser Gly Thr Thr Gly Leu Pro Lys Gly Val
195 200 205
Met Leu Thr His Arg Ser Leu Val Thr Ser Val Ala Gln Gln Val Asp
210 215 220
Gly Glu Asn Pro Asn Leu Tyr Phe Arg Arg Glu Asp Val Val Leu Cys
225 230 235 240
Leu Leu Pro Leu Phe His Ile Tyr Ser Leu Asn Ser Val Leu Leu Ala
245 250 255
Gly Leu Arg Ala Gly Ser Ala Ile Val Ile Met Arg Lys Phe Asp Leu
260 265 270
Gly Ala Leu Val Asp Leu Thr Arg Arg His Gly Val Thr Val Ala Pro
275 280 285
Phe Val Pro Pro Ile Val Val Glu Ile Ala Lys Ser Pro Arg Val Thr
290 295 300
Ala Asp Asp Leu Ala Ser Ile Arg Met Val Met Ser Gly Ala Ala Pro
305 310 315 320
Met Gly Lys Asp Leu Gln Asp Ala Phe Met Ala Lys Ile Pro Asn Ala
325 330 335
Val Leu Gly Gln Gly Tyr Gly Met Thr Glu Ala Gly Pro Val Leu Ala
340 345 350
Met Cys Leu Ala Phe Ala Lys Glu Pro Phe Glu Val Lys Ser Gly Ser
355 360 365
Cys Gly Thr Val Val Arg Asn Ala Glu Leu Lys Ile Val Asp Pro Asp
370 375 380
Thr Gly Ala Thr Leu Gly Arg Asn Gln Ser Gly Glu Ile Cys Ile Arg
385 390 395 400
Gly Glu Gln Ile Met Lys Gly Tyr Leu Asn Asp Pro Glu Ser Thr Lys
405 410 415
Asn Thr Ile Asp Lys Gly Gly Trp Leu His Thr Gly Asp Ile Gly Tyr
420 425 430
Val Asp Asp Asp Asp Glu Ile Phe Ile Val Asp Arg Leu Lys Glu Ile
435 440 445
Ile Lys Tyr Lys Gly Phe Gln Val Pro Pro Ala Glu Leu Glu Ala Leu
450 455 460
Leu Ile Thr His Pro Asp Ile Lys Asp Ala Ala Val Val Pro Met Ile
465 470 475 480
Asp Glu Ile Ala Gly Glu Val Pro Val Ala Phe Ile Val Arg Ile Glu
485 490 495
Gly Ser Ala Ile Ser Glu Asn Glu Ile Lys Gln Phe Val Ala Lys Glu
500 505 510
Val Val Phe Tyr Lys Arg Leu Asn Lys Val Phe Phe Ala Asp Ser Ile
515 520 525
Pro Lys Ser Pro Ser Gly Lys Ile Leu Arg Lys Asp Leu Arg Ala Lys
530 535 540
Leu Ala Ala Gly Ile Pro Thr Asn Asp Asn Thr Gln Leu Lys Ser
545 550 555
<210> 151
<211> 557
<212> PRT
<213> 黑麦草()
<220>
<223> 黑麦草4-香豆酸-CoA连接酶 (Lp4CL3)
<400> 151
Met Gly Ser Val Pro Glu Glu Ser Val Val Ala Val Ala Pro Ala Glu
1 5 10 15
Thr Val Phe Arg Ser Lys Leu Pro Asp Ile Glu Ile Asn Asn Glu Gln
20 25 30
Thr Leu Gln Ser Tyr Cys Phe Glu Lys Met Ala Glu Val Ala Ser Arg
35 40 45
Pro Cys Ile Ile Asp Gly Gln Thr Gly Ala Ser Tyr Thr Tyr Thr Glu
50 55 60
Val Asp Ser Leu Thr Arg Arg Ala Ala Ala Gly Leu Arg Arg Met Gly
65 70 75 80
Val Gly Lys Gly Asp Val Val Met Asn Leu Leu Arg Asn Cys Pro Glu
85 90 95
Phe Ala Phe Ser Phe Leu Gly Ala Ala Arg Leu Gly Ala Ala Thr Thr
100 105 110
Thr Ala Asn Pro Phe Tyr Thr Pro His Glu Ile His Arg Gln Ala Glu
115 120 125
Ala Ala Gly Ala Lys Leu Ile Val Thr Glu Ala Cys Ala Val Glu Lys
130 135 140
Val Leu Glu Phe Ala Ala Gly Arg Gly Val Pro Val Val Thr Val Asp
145 150 155 160
Gly Arg Arg Asp Gly Cys Val Asp Phe Ala Glu Leu Ile Ala Gly Glu
165 170 175
Glu Leu Pro Glu Ala Asp Glu Ala Gly Val Leu Pro Asp Asp Val Val
180 185 190
Ala Leu Pro Tyr Ser Ser Gly Thr Thr Gly Leu Pro Lys Gly Val Met
195 200 205
Leu Thr His Arg Ser Leu Val Thr Ser Val Ala Gln Leu Val Asp Gly
210 215 220
Ser Asn Pro Asn Val Cys Phe Asn Lys Asp Asp Ala Leu Leu Cys Leu
225 230 235 240
Leu Pro Leu Phe His Ile Tyr Ser Leu His Thr Val Leu Leu Ala Gly
245 250 255
Leu Arg Val Gly Ala Ala Ile Val Ile Met Arg Lys Phe Asp Val Gly
260 265 270
Ala Leu Val Asp Leu Val Arg Ala His Arg Ile Thr Ile Ala Pro Phe
275 280 285
Val Pro Pro Ile Val Val Glu Ile Ala Lys Ser Asp Arg Val Gly Ala
290 295 300
Asp Asp Leu Ala Ser Ile Arg Met Val Leu Ser Gly Ala Ala Pro Met
305 310 315 320
Gly Lys Asp Leu Gln Asp Ala Phe Met Ala Lys Ile Pro Asn Ala Val
325 330 335
Leu Gly Gln Gly Tyr Gly Met Thr Glu Ala Gly Pro Val Leu Ala Met
340 345 350
Cys Leu Ala Phe Ala Lys Glu Pro Phe Lys Val Lys Ser Gly Ser Cys
355 360 365
Gly Thr Val Val Arg Asn Ala Glu Leu Lys Val Val Asp Pro Asp Thr
370 375 380
Gly Ala Ser Leu Gly Arg Asn Gln Pro Gly Glu Ile Cys Val Arg Gly
385 390 395 400
Lys Gln Ile Met Ile Gly Tyr Leu Asn Asp Pro Glu Ser Thr Lys Asn
405 410 415
Thr Ile Asp Lys Asp Gly Trp Leu His Thr Gly Asp Ile Gly Leu Val
420 425 430
Asp Asp Asp Asp Glu Ile Phe Ile Val Asp Arg Leu Lys Glu Ile Ile
435 440 445
Lys Tyr Lys Gly Phe Gln Val Ala Pro Ala Glu Leu Glu Ala Leu Leu
450 455 460
Leu Thr Asn Pro Glu Val Lys Asp Ala Ala Val Val Gly Val Lys Asp
465 470 475 480
Asp Leu Cys Gly Glu Val Pro Val Ala Phe Ile Lys Arg Ile Glu Gly
485 490 495
Ser Glu Ile Asn Glu Asn Glu Ile Lys Gln Phe Val Ser Lys Glu Val
500 505 510
Val Phe Tyr Lys Arg Ile Asn Lys Val Tyr Phe Thr Asp Ser Ile Pro
515 520 525
Lys Asn Pro Ser Gly Lys Ile Leu Arg Lys Asp Leu Arg Ala Arg Leu
530 535 540
Ala Ala Gly Ile Pro Thr Glu Val Ala Ala Pro Arg Ser
545 550 555
<210> 152
<211> 537
<212> PRT
<213> 江南卷柏()
<220>
<223> 卷柏4-香豆酸-CoA连接酶 (Sm4CL1)
<400> 152
Met Ala Thr Asp Gln His Val Ala Ala Glu Tyr Ile Phe Arg Ser Lys
1 5 10 15
Leu Pro Asp Ile Gln Ile Pro Asn His Val Pro Leu Ala Thr Tyr Cys
20 25 30
Leu Glu Arg Ala Ala Ala Asp Pro Glu Leu Gly Gly Lys Ala Ala Leu
35 40 45
Ile Asp Gly Pro Thr Gly Thr Gln Met Ser Tyr Ala Gln Leu Gly Val
50 55 60
Ala Ile Arg Arg Val Gly Ala Gly Leu Ala Lys Leu Gly Ile Gly Arg
65 70 75 80
Gly Glu Val Val Met Leu Leu Leu Pro Asn Ser Ile Glu Phe Val Leu
85 90 95
Val Phe Leu Gly Cys Ala Val Arg Gly Cys Val Ala Thr Thr Ala Asn
100 105 110
Pro Phe Tyr Thr Pro Pro Glu Ile Ala Lys Gln Ala Lys Ala Ser Gly
115 120 125
Thr Lys Leu Ile Val Thr Leu Ser Thr Tyr Val Asp Lys Val Ser Gly
130 135 140
Ile Ala Glu Val Met Ser Ile Asp Arg Glu Val Glu Gly Cys Leu His
145 150 155 160
Ile Ser Ala Leu Thr Gln Ala Asp Glu Gly Glu Cys Pro Ala Val Asp
165 170 175
Ile Gln Pro Asp Asp Val Val Ala Leu Pro Phe Ser Ser Gly Thr Thr
180 185 190
Gly Leu Pro Lys Gly Val Met Leu Thr His Lys Ser Leu Val Ser Ser
195 200 205
Ile Ala Gln Gln Val Asp Gly Asp Asn Pro Asn Leu Tyr Met Thr Pro
210 215 220
Ser Asp Ala Val Leu Cys Val Leu Pro Met Phe His Ile Tyr Ser Leu
225 230 235 240
Asn Ser Ile Leu Leu Cys Ser Leu Arg Thr Ala Ser Thr Ile Val Ile
245 250 255
Met Pro Lys Phe Asp Leu Thr Gln Leu Leu Glu Leu Val Thr Arg Tyr
260 265 270
Ser Ile Ser Ile Ala Pro Ile Val Pro Pro Ile Val Leu Ala Leu Ala
275 280 285
Lys Asn Pro Ala Val Leu Ala Tyr Asp Leu Ser Ser Ile Arg Met Val
290 295 300
Gln Ser Gly Ala Ala Pro Leu Gly Lys Glu Ile Glu Asp Ala Phe Arg
305 310 315 320
Ala Arg Leu Pro Arg Ala Thr Ile Gly Gln Gly Tyr Gly Met Thr Glu
325 330 335
Ala Gly Pro Val Val Ala Leu Cys Leu Ala Phe Ala Lys His Pro Phe
340 345 350
Thr Val Lys Pro Gly Ser Cys Gly Thr Ile Val Arg Asn Ala Asp Ala
355 360 365
Lys Ile Val Asp Pro Glu Thr Gly Ala Ser Leu Pro Arg Asn Gln Pro
370 375 380
Gly Glu Met Cys Ile Arg Gly Pro Gln Val Met Lys Gly Tyr Leu Gly
385 390 395 400
Asp Pro Glu Ser Thr Arg Ser Thr Val Asp Lys Asp Gly Trp Leu His
405 410 415
Thr Gly Asp Val Ala Leu Ile Asp Asp Asp Asp Glu Val Phe Ile Val
420 425 430
Asp Arg Val Lys Glu Ile Ile Lys Tyr Lys Gly Phe Gln Val Ala Pro
435 440 445
Ala Glu Leu Glu Ala Leu Leu Ile Ser Asn Pro Ser Ile Ala Asp Ala
450 455 460
Ala Val Val Ala Lys Lys Asp Asp Leu Thr Gly Glu Val Pro Val Ala
465 470 475 480
Phe Val Val Arg Ala Ala Asp Ser His Ile Ser Glu Asp Asp Ile Lys
485 490 495
Gly Phe Val Ser Lys Gln Val Val Phe Tyr Lys Lys Ile His Ser Val
500 505 510
Tyr Phe Val Asp Ser Ile Pro Lys Asn Pro Ser Gly Lys Ile Leu Arg
515 520 525
Lys Glu Leu Arg Ser Arg Leu Ser Ser
530 535
<210> 153
<211> 585
<212> PRT
<213> 小立碗藓()
<220>
<223> 藓4-香豆酸-CoA连接酶 (Pp4CL1)
<400> 153
Met Ser Pro Ser Leu Leu Pro Gln Pro Thr Val Ser Glu Ser Thr Gly
1 5 10 15
Glu Ser Val Met Lys Lys Ser Leu Gln Ser Glu Val Arg Glu Ala Ser
20 25 30
Leu Ala Ile Gly Glu Asn Leu Glu Pro Phe Leu Ser Glu Thr Asp Ala
35 40 45
Glu Ser Gln Ile Met Glu Pro Val His Ala Glu Val His Asp Phe Ile
50 55 60
Tyr Arg Ser Lys Leu Pro Asp Ile Asp Ile Pro Asn His Met Pro Leu
65 70 75 80
Ala Asp Tyr Cys Leu Glu Lys Ser Ser Gln Trp Pro Asp Lys Val Cys
85 90 95
Leu Ile Asp Gly Val Thr Gly Arg Glu His Arg Tyr Gly Glu Ile Glu
100 105 110
Leu Ser Ser Arg Arg Val Ala Ala Gly Leu Asp Lys Ile Gly Val Lys
115 120 125
Gln Gly Asp Val Ile Ala Leu Leu Leu Pro Asn Cys Ala Glu Phe Val
130 135 140
Leu Val Phe Leu Gly Ala Ala Lys Arg Gly Ala Val Val Thr Thr Ala
145 150 155 160
Asn Pro Phe Tyr Thr Ala Ala Glu Leu Glu Lys Gln Ile Glu Ala Ser
165 170 175
Gly Ala Gly Ile Val Ile Thr Gln Ser Ser Tyr Val Glu Lys Leu Ala
180 185 190
Gly Leu Asn Val Gln Ile Ile Thr Val Asp Gln His Val Ala Asn Cys
195 200 205
Met His Ile Ser Val Leu Leu Asn Ala Cys Glu Gly Glu Cys Pro Gln
210 215 220
Val Arg Ile His Pro Asp Asp Leu Val Cys Leu Pro Tyr Ser Ser Gly
225 230 235 240
Thr Thr Gly Leu Pro Lys Gly Val Met Leu Thr His Lys Ser Leu Val
245 250 255
Ser Ser Val Ser Gln Gln Val Asp Gly Glu Ala Pro Asn Phe Asn Ile
260 265 270
Thr Val Glu Asp Thr Leu Met Cys Val Leu Pro Met Phe His Ile Tyr
275 280 285
Ser Leu Asn Ser Ile Leu Leu Cys Gly Leu Arg Val Gly Ala Thr Leu
290 295 300
Val Ile Met Pro Lys Phe Glu Leu Pro Lys Leu Leu Asp Leu Ile Gln
305 310 315 320
Arg His Lys Val Thr Met Gly Pro Leu Val Pro Pro Ile Val Leu Ala
325 330 335
Ile Ala Lys Asn Pro Ile Val Glu Asn Tyr Asp Leu Ser Ser Met Arg
340 345 350
Met Val Met Ser Gly Ala Ala Pro Leu Gly Lys Glu Leu Glu Asp Ala
355 360 365
Phe Arg Ala Arg Leu Pro Asn Ala Val Leu Gly Gln Gly Tyr Gly Met
370 375 380
Thr Glu Ala Gly Pro Val Leu Ala Met Cys Leu Ala Phe Ala Lys Thr
385 390 395 400
Pro Phe Ser Val Lys Pro Gly Ser Cys Gly Thr Val Val Arg Asn Ala
405 410 415
Glu Val Lys Ile Val Asp Thr Glu Thr Gly Met Ser Leu Pro Tyr Asn
420 425 430
Gln Pro Gly Glu Ile Cys Ile Arg Gly Pro Gln Ile Met Lys Gly Tyr
435 440 445
Leu Lys Asn Pro Glu Ala Thr Ala Asn Thr Ile Asp Lys Asp Gly Phe
450 455 460
Leu His Thr Gly Asp Val Ala Phe Ile Asp Glu Asp Glu Glu Met Phe
465 470 475 480
Ile Val Asp Arg Val Lys Glu Ile Ile Lys Phe Lys Gly Phe Gln Val
485 490 495
Pro Pro Ala Glu Leu Glu Ala Leu Leu Ile Ser His Lys Glu Ile Gln
500 505 510
Asp Ala Ala Val Val Ser Arg Lys Asp Asp Val Ala Gly Glu Val Pro
515 520 525
Val Ala Phe Val Val Arg Ala Thr Ser Ser Thr Ile Ser Glu Asp Glu
530 535 540
Val Lys Asp Tyr Ile Ala Lys Gln Val Val Phe Tyr Lys Lys Ile His
545 550 555 560
Asn Val Tyr Phe Val Asp Ser Ile Pro Lys Ser Pro Ser Gly Lys Ile
565 570 575
Leu Arg Lys Asp Leu Arg Asn Lys Val
580 585
<210> 154
<211> 432
<212> PRT
<213> 人工序列()
<220>
<223> 合成的羟基肉桂酰辅酶A:莽草酸羟基肉桂酰基转移酶 (HCT) 共有序列（大多数）
<220>
<221> 变体
<222> (7)..(.7)
<223> Xaa = Asp, Glu, Arg, Gly或Ser
<220>
<221> 变体
<222> (48)..(.48)
<223> Xaa = Ser, Ala, Pro, Glu, Gly或Cys
<220>
<221> 变体
<222> (54)..(.54)
<223> Xaa = Gln, Lys, Asp, Glu, Ala或Gly
<220>
<221> 变体
<222> (61)..(.61)
<223> Xaa = Ser, Gly或Ala
<220>
<221> 变体
<222> (75)..(.75)
<223> Xaa = Lys, Arg, Cys或Ala
<220>
<221> 变体
<222> (197)..(.197)
<223> Xaa = Ala, Gln, Val, Ser, Thr或Lys
<220>
<221> 变体
<222> (211)..(.211)
<223> Xaa = Ile, Thr, Ala, Val, Ser, Gly, His或Gln
<220>
<221> 变体
<222> (213)..(.213)
<223> Xaa = Leu, Ala, Pro, Gln或Glu
<220>
<221> 变体
<222> (214)..(.214)
<223> Xaa = Asp, Glu, Gln, Pro, Thr, Val或Ala
<220>
<221> 变体
<222> (215)..(.215)
<223> Xaa = Pro, Thr, Asn或Ser
<220>
<221> 变体
<222> (221)..(.221)
<223> Xaa = Asn, Ser, Glu, Gly, Val, Pro, Ala或Arg
<220>
<221> 变体
<222> (232)..(.232)
<223> Xaa = Asp, Glu, Thr或Ser
<220>
<221> 变体
<222> (241)..(.241)
<223> Xaa = Ser, Ala, Gly或Leu
<220>
<221> 变体
<222> (247)..(.247)
<223> Xaa = Thr, Ile, Asn, Gly或Ala
<220>
<221> 变体
<222> (263)..(.263)
<223> Xaa = Ser, Cys或Trp
<220>
<221> 变体
<222> (286)..(.286)
<223> Xaa = Ser, Ala, Gly或Gln
<220>
<221> 变体
<222> (309)..(.309)
<223> Xaa = Val, Ile, Glu或Leu
<220>
<221> 变体
<222> (323)..(.323)
<223> Xaa = Gly, Ser, Ala或Thr
<220>
<221> 变体
<222> (330)..(.330)
<223> Xaa = Val, Ala, Ser, Asp或Glu
<220>
<221> 变体
<222> (386)..(.386)
<223> Xaa = Ile, Arg或Val
<220>
<221> 变体
<222> (399)..(.399)
<223> Xaa = Ser, Gly, Ala或Val
<220>
<221> UNSURE
<222> (7)..(7)
<223> The 'Xaa' at location 7 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (48)..(48)
<223> The 'Xaa' at location 48 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (54)..(54)
<223> The 'Xaa' at location 54 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (61)..(61)
<223> The 'Xaa' at location 61 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (75)..(75)
<223> The 'Xaa' at location 75 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (197)..(197)
<223> The 'Xaa' at location 197 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (211)..(211)
<223> The 'Xaa' at location 211 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (213)..(213)
<223> The 'Xaa' at location 213 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (214)..(214)
<223> The 'Xaa' at location 214 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (215)..(215)
<223> The 'Xaa' at location 215 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (221)..(221)
<223> The 'Xaa' at location 221 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (232)..(232)
<223> The 'Xaa' at location 232 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (241)..(241)
<223> The 'Xaa' at location 241 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (247)..(247)
<223> The 'Xaa' at location 247 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (263)..(263)
<223> The 'Xaa' at location 263 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (286)..(286)
<223> The 'Xaa' at location 286 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (309)..(309)
<223> The 'Xaa' at location 309 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (323)..(323)
<223> The 'Xaa' at location 323 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (330)..(330)
<223> The 'Xaa' at location 330 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (386)..(386)
<223> The 'Xaa' at location 386 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (399)..(399)
<223> The 'Xaa' at location 399 stands for Gln, Arg, Pro, or Leu.
<400> 154
Met Lys Ile Asn Val Arg Xaa Ser Thr Met Val Arg Pro Ala Glu Glu
1 5 10 15
Thr Pro Arg Arg Arg Leu Trp Asn Ser Asn Val Asp Leu Val Val Pro
20 25 30
Arg Phe His Thr Pro Ser Val Tyr Phe Tyr Arg Pro Thr Gly Ala Xaa
35 40 45
Asn Phe Phe Asp Gly Xaa Val Met Lys Glu Ala Leu Xaa Lys Ala Leu
50 55 60
Val Pro Phe Tyr Pro Met Ala Gly Arg Leu Xaa Arg Asp Glu Asp Gly
65 70 75 80
Arg Ile Glu Ile Asp Cys Asn Gly Glu Gly Val Leu Phe Val Glu Ala
85 90 95
Asp Ala Asp Ala Val Val Asp Asp Phe Gly Asp Phe Ala Pro Thr Leu
100 105 110
Glu Leu Lys Arg Leu Ile Pro Thr Val Asp Tyr Thr Asp Gly Ile Ser
115 120 125
Ser Phe Pro Leu Leu Val Leu Gln Val Thr Tyr Phe Lys Cys Gly Gly
130 135 140
Val Ser Leu Gly Val Gly Met Gln His His Ala Ala Asp Gly Phe Ser
145 150 155 160
Gly Leu His Phe Ile Asn Thr Trp Ser Asp Met Ala Arg Gly Leu Asp
165 170 175
Ile Thr Ile Pro Pro Phe Ile Asp Arg Thr Leu Leu Arg Ala Arg Asp
180 185 190
Pro Pro Gln Pro Xaa Phe Pro His Ile Glu Tyr Gln Pro Ala Pro Ala
195 200 205
Met Lys Xaa Ser Xaa Xaa Xaa Gln Lys Ala Pro Pro Xaa Thr Ala Val
210 215 220
Ser Ile Phe Lys Leu Thr Arg Xaa Gln Leu Gly Arg Leu Lys Ala Lys
225 230 235 240
Xaa Lys Glu Gly Glu Asn Xaa Pro Arg Tyr Ser Ser Tyr Glu Met Leu
245 250 255
Ala Gly His Val Trp Arg Xaa Val Cys Leu Ala Arg Gly Leu Pro Asp
260 265 270
Asp Gln Glu Thr Lys Leu Tyr Ile Ala Thr Asp Gly Arg Xaa Arg Leu
275 280 285
Arg Pro Pro Leu Pro Pro Gly Tyr Phe Gly Asn Val Ile Phe Thr Ala
290 295 300
Thr Pro Leu Ala Xaa Ala Gly Asp Leu Thr Ser Lys Pro Leu Trp Tyr
305 310 315 320
Ala Ala Xaa Val Ile His Asp Ala Leu Xaa Arg Met Asp Asn Asp Tyr
325 330 335
Leu Arg Ser Ala Leu Asp Tyr Leu Glu Leu Gln Pro Asp Leu Ser Ala
340 345 350
Leu Val Arg Gly Ala His Thr Phe Arg Cys Pro Asn Leu Gly Ile Thr
355 360 365
Ser Trp Val Arg Leu Pro Ile His Asp Ala Asp Phe Gly Trp Gly Arg
370 375 380
Pro Xaa Phe Met Gly Pro Gly Gly Ile Ala Tyr Glu Gly Leu Xaa Phe
385 390 395 400
Val Leu Pro Ser Pro Thr Asn Asp Gly Ser Leu Ser Val Ala Ile Ser
405 410 415
Leu Gln Ala Glu His Met Lys Leu Phe Glu Lys Phe Ile Tyr Glu Ile
420 425 430
<210> 155
<211> 433
<212> PRT
<213> 琴叶拟南芥()
<220>
<223> 羟基肉桂酰辅酶A:莽草酸羟基肉桂酰基转移酶 (AlHCT)
<400> 155
Met Lys Ile Asn Ile Arg Asp Ser Thr Met Val Arg Pro Ala Ala Glu
1 5 10 15
Thr Pro Ile Thr Asn Leu Trp Asn Ser Asn Val Asp Leu Val Ile Pro
20 25 30
Arg Phe His Thr Pro Ser Val Tyr Phe Tyr Arg Pro Thr Gly Ala Ser
35 40 45
Asn Phe Phe Asp Pro Gln Ile Met Lys Asp Ala Leu Ser Lys Ala Leu
50 55 60
Val Pro Phe Tyr Pro Met Ala Gly Arg Leu Lys Arg Asp Asp Asp Gly
65 70 75 80
Arg Ile Glu Ile Asp Cys Asn Gly Ala Gly Val Leu Phe Val Val Ala
85 90 95
Asp Thr Pro Ser Val Ile Asp Asp Phe Gly Asp Phe Ala Pro Thr Leu
100 105 110
Asn Leu Arg Gln Leu Ile Pro Asp Val Asp His Ser Ala Gly Ile His
115 120 125
Ser Phe Pro Leu Leu Val Leu Gln Val Thr Phe Phe Lys Cys Gly Gly
130 135 140
Ala Ser Leu Gly Val Gly Met Gln His His Ala Ala Asp Gly Phe Ser
145 150 155 160
Gly Leu His Phe Ile Asn Thr Trp Ser Asp Met Ala Arg Gly Leu Asp
165 170 175
Leu Thr Ile Pro Pro Phe Ile Asp Arg Thr Leu Leu Arg Ala Arg Asp
180 185 190
Pro Pro Gln Pro Ala Phe His His Val Glu Tyr Gln Pro Ala Pro Ser
195 200 205
Met Arg Ile Pro Leu Asp Pro Ser Lys Ser Gly Pro Asp Asn Thr Thr
210 215 220
Val Ser Ile Phe Lys Leu Ser Arg Asp Gln Leu Val Ala Leu Lys Ala
225 230 235 240
Lys Ser Lys Glu Asp Gly Asn Ile Val Ser Tyr Ser Ser Tyr Glu Met
245 250 255
Leu Ala Gly His Val Trp Arg Ser Val Gly Lys Ala Arg Gly Leu Pro
260 265 270
Asp Asp Gln Glu Thr Lys Leu Tyr Ile Ala Thr Asp Gly Arg Ser Arg
275 280 285
Leu Arg Pro Gln Leu Pro Pro Gly Tyr Phe Gly Asn Val Ile Phe Thr
290 295 300
Ala Thr Pro Leu Ala Val Ala Gly Asp Leu Leu Ser Lys Pro Thr Trp
305 310 315 320
Tyr Ala Ala Gly Leu Ile His Asp Val Leu Val Arg Met Asp Asp Asn
325 330 335
Tyr Leu Arg Ser Ala Leu Asp Tyr Leu Glu Met Gln Pro Asp Leu Ser
340 345 350
Ala Leu Val Arg Gly Ala His Thr Tyr Lys Cys Pro Asn Leu Gly Ile
355 360 365
Thr Ser Trp Val Arg Leu Pro Ile Tyr Asp Ala Asp Phe Gly Trp Gly
370 375 380
Arg Pro Ile Phe Met Gly Pro Gly Gly Ile Pro Tyr Glu Gly Leu Ser
385 390 395 400
Phe Val Leu Pro Ser Pro Thr Asn Asp Gly Ser Leu Ser Val Ala Ile
405 410 415
Ala Leu Gln Ser Glu His Met Lys Leu Phe Glu Lys Phe Leu Tyr Glu
420 425 430
Ile
<210> 156
<211> 457
<212> PRT
<213> 火炬松()
<220>
<223> 松树羟基肉桂酰辅酶A:莽草酸羟基肉桂酰基转移酶 (PtHCT)
<400> 156
Met Leu Pro Leu His Arg Thr Trp Gly Gly Arg Gly Ile Glu Ser Ile
1 5 10 15
Asn Arg Ala Leu Ala Arg Gly Lys Met Ile Ile Asn Leu Lys Glu Ser
20 25 30
Thr Met Val Gln Pro Ala Glu Glu Thr Pro Arg Arg Gly Leu Trp Asn
35 40 45
Ser Asn Val Asp Leu Val Val Pro Arg Phe His Thr Pro Ser Val Tyr
50 55 60
Phe Tyr Arg Pro Thr Gly Ala Ser Asn Phe Phe Asp Ala Lys Val Leu
65 70 75 80
Lys Glu Ala Leu Ser Lys Ala Leu Val Pro Phe Tyr Pro Met Ala Gly
85 90 95
Arg Leu Arg Arg Asp Asp Asp Gly Arg Ile Glu Ile Asp Cys Asn Ala
100 105 110
Glu Gly Val Leu Phe Val Glu Ala Gly Thr Ala Ser Val Val Ala Asp
115 120 125
Phe Gly Asp Phe Ala Pro Thr Leu Glu Leu Lys Gln Leu Ile Pro Thr
130 135 140
Val Asp Tyr Ser Gly Gly Ile Ser Thr Tyr Pro Leu Leu Val Leu Gln
145 150 155 160
Val Thr Tyr Phe Lys Cys Gly Gly Val Ser Leu Gly Val Gly Met Gln
165 170 175
His His Ala Ala Asp Gly Phe Ser Gly Leu His Phe Val Asn Thr Trp
180 185 190
Ser Asp Met Ala Arg Gly Leu Asp Leu Thr Ile Pro Pro Phe Ile Asp
195 200 205
Arg Thr Leu Leu Arg Ala Arg Asp Pro Pro Gln Pro Ala Phe His His
210 215 220
Val Glu Tyr Gln Pro Pro Pro Ala Met Lys Thr Val Leu Glu Thr Ser
225 230 235 240
Lys Pro Glu Ser Thr Ala Val Ser Ile Phe Lys Leu Thr Arg Asp Gln
245 250 255
Leu Asn Thr Leu Lys Ala Lys Ala Lys Glu Gly Gly Asn Asn Ile Gly
260 265 270
Tyr Ser Ser Tyr Glu Met Leu Ala Gly His Val Trp Arg Ser Ala Cys
275 280 285
Lys Ala Arg Gly Leu Pro Asp Asp Gln Glu Thr Lys Leu Tyr Ile Ala
290 295 300
Thr Asp Gly Arg Ser Arg Leu Arg Pro Thr Leu Pro Pro Gly Tyr Phe
305 310 315 320
Gly Asn Val Ile Phe Thr Ala Thr Pro Ile Ala Val Ala Gly Glu Ile
325 330 335
Gln Ser Lys Pro Thr Trp Tyr Ala Ala Gly Lys Ile His Asp Ser Leu
340 345 350
Val Arg Met Asp Asn Asp Tyr Leu Arg Ser Ala Leu Asp Phe Leu Glu
355 360 365
Leu Gln Pro Asp Leu Ser Ala Leu Val Arg Gly Ala His Thr Phe Arg
370 375 380
Cys Pro Asn Leu Gly Ile Thr Ser Trp Val Arg Leu Pro Ile His Asp
385 390 395 400
Ala Asp Phe Gly Trp Gly Arg Pro Ile Phe Met Gly Pro Gly Gly Ile
405 410 415
Ala Tyr Glu Gly Leu Ser Phe Ile Ile Pro Ser Ser Thr Asn Asp Gly
420 425 430
Ser Leu Ser Val Ala Ile Ser Leu Gln Ala Glu His Met Lys Leu Phe
435 440 445
Glu Lys Phe Ile Tyr Asp Ile Lys Glu
450 455
<210> 157
<211> 431
<212> PRT
<213> 蓖麻()
<220>
<223> 羟基肉桂酰辅酶A: 莽草酸松羟基肉桂酰基转移酶 (RcHCT)
<400> 157
Met Ile Ile Asn Val Lys Glu Ser Thr Leu Val Pro Pro Ser Glu Glu
1 5 10 15
Thr Pro Arg Val Gly Leu Trp Asn Ser Asn Val Asp Leu Val Val Pro
20 25 30
Arg Phe His Thr Pro Ser Val Tyr Phe Tyr Arg Pro Thr Gly Ala Ser
35 40 45
Asn Phe Phe Asp Pro Asp Val Leu Lys Ala Ala Leu Gly Lys Ala Leu
50 55 60
Val Pro Phe Tyr Pro Met Ala Gly Arg Leu Arg Arg Asp Glu Asp Gly
65 70 75 80
Arg Ile Glu Ile Asp Cys Asn Ala Glu Gly Val Leu Leu Val Val Ala
85 90 95
Glu Thr Asn Ala Val Val Asp Asp Phe Gly Asp Phe Ala Pro Thr Leu
100 105 110
Glu Leu Lys Gln Leu Ile Pro Thr Val Asp Tyr Ser Gly Gly Ile Ser
115 120 125
Thr Phe Pro Leu Leu Val Leu Gln Val Thr His Phe Lys Cys Gly Gly
130 135 140
Val Ser Leu Gly Val Gly Met Gln His His Ala Ala Asp Gly Phe Ser
145 150 155 160
Gly Leu His Phe Val Asn Thr Trp Ser Asp Met Ala Arg Gly Leu Asp
165 170 175
Cys Thr Ile Pro Pro Phe Ile Asp Arg Thr Leu Leu Arg Ala Arg Asp
180 185 190
Pro Pro Gln Pro Gln Phe Asn His Ile Glu Tyr Gln Pro Pro Pro Ala
195 200 205
Leu Lys Ala Ser Ala Glu Thr Leu Lys Pro Asp Ser Thr Thr Val Ser
210 215 220
Ile Phe Lys Leu Ser Arg Asp Gln Leu Asn Ile Leu Lys Ala Lys Ala
225 230 235 240
Lys Glu Asp Gly Asn Thr Ile Asn Tyr Ser Ser Tyr Ala Met Leu Ala
245 250 255
Gly His Val Trp Arg Ser Ala Cys Lys Ala Arg Gly Leu Thr Asp Asp
260 265 270
Gln Glu Thr Lys Leu Tyr Ile Ala Thr Asp Gly Arg Ser Arg Leu Asn
275 280 285
Pro Pro Leu Pro Pro Gly Tyr Phe Gly Asn Val Ile Phe Thr Ala Thr
290 295 300
Pro Ile Ala Val Ala Gly Asp Leu Glu Ser Lys Pro Thr Trp Tyr Ala
305 310 315 320
Ala Gly Lys Ile His Asp Ala Leu Ala Arg Met Asp Asn Asp Tyr Leu
325 330 335
Arg Ser Ala Leu Asp Phe Leu Glu Leu Gln Pro Asp Leu Ser Ala Leu
340 345 350
Val Arg Gly Ala His Thr Phe Lys Cys Pro Asn Leu Gly Ile Thr Ser
355 360 365
Trp Val Arg Leu Pro Ile His Asp Ala Asp Phe Gly Trp Gly Arg Pro
370 375 380
Ile Phe Met Gly Pro Gly Gly Ile Ala Tyr Glu Gly Leu Ser Phe Ile
385 390 395 400
Leu Pro Ser Pro Thr Asn Asp Gly Ser Leu Ser Val Ala Ile Ala Leu
405 410 415
Gln Ser Glu His Met Lys Leu Phe Glu Lys Phe Ile Tyr Glu Leu
420 425 430
<210> 158
<211> 434
<212> PRT
<213> 中果咖啡()
<220>
<223> 羟基肉桂酰辅酶A:莽草酸羟基肉桂酰基转移酶 (CcHCT)
<400> 158
Met Lys Ile Glu Val Lys Glu Ser Thr Met Val Arg Pro Ala Gln Glu
1 5 10 15
Thr Pro Gly Arg Asn Leu Trp Asn Ser Asn Val Asp Leu Val Val Pro
20 25 30
Asn Phe His Thr Pro Ser Val Tyr Phe Tyr Arg Pro Thr Gly Ser Ser
35 40 45
Asn Phe Phe Asp Ala Lys Val Leu Lys Asp Ala Leu Ser Arg Ala Leu
50 55 60
Val Pro Phe Tyr Pro Met Ala Gly Arg Leu Lys Arg Asp Glu Asp Gly
65 70 75 80
Arg Ile Glu Ile Glu Cys Asn Gly Glu Gly Val Leu Phe Val Glu Ala
85 90 95
Glu Ser Asp Gly Val Val Asp Asp Phe Gly Asp Phe Ala Pro Thr Leu
100 105 110
Glu Leu Arg Arg Leu Ile Pro Ala Val Asp Tyr Ser Gln Gly Ile Ser
115 120 125
Ser Tyr Ala Leu Leu Val Leu Gln Val Thr Tyr Phe Lys Cys Gly Gly
130 135 140
Val Ser Leu Gly Val Gly Met Arg His His Ala Ala Asp Gly Phe Ser
145 150 155 160
Gly Leu His Phe Ile Asn Ser Trp Ser Asp Met Ala Arg Gly Leu Asp
165 170 175
Val Thr Leu Pro Pro Phe Ile Asp Arg Thr Leu Leu Arg Ala Arg Asp
180 185 190
Pro Pro Gln Pro Gln Phe Gln His Ile Glu Tyr Gln Pro Pro Pro Ala
195 200 205
Leu Lys Val Ser Pro Gln Thr Ala Lys Ser Asp Ser Val Pro Glu Thr
210 215 220
Ala Val Ser Ile Phe Lys Leu Thr Arg Glu Gln Ile Ser Ala Leu Lys
225 230 235 240
Ala Lys Ser Lys Glu Asp Gly Asn Thr Ile Ser Tyr Ser Ser Tyr Glu
245 250 255
Met Leu Ala Gly His Val Trp Arg Cys Ala Cys Lys Ala Arg Gly Leu
260 265 270
Glu Val Asp Gln Gly Thr Lys Leu Tyr Ile Ala Thr Asp Gly Arg Ala
275 280 285
Arg Leu Arg Pro Ser Leu Pro Pro Gly Tyr Phe Gly Asn Val Ile Phe
290 295 300
Thr Ala Thr Pro Ile Ala Ile Ala Gly Asp Leu Glu Phe Lys Pro Val
305 310 315 320
Trp Tyr Ala Ala Ser Lys Ile His Asp Ala Leu Ala Arg Met Asp Asn
325 330 335
Asp Tyr Leu Arg Ser Ala Leu Asp Tyr Leu Glu Leu Gln Pro Asp Leu
340 345 350
Lys Ala Leu Val Arg Gly Ala His Thr Phe Lys Cys Pro Asn Leu Gly
355 360 365
Ile Thr Ser Trp Val Arg Leu Pro Ile His Asp Ala Asp Phe Gly Trp
370 375 380
Gly Arg Pro Ile Phe Met Gly Pro Gly Gly Ile Ala Tyr Glu Gly Leu
385 390 395 400
Ser Phe Ile Leu Pro Ser Pro Thr Asn Asp Gly Ser Met Ser Val Ala
405 410 415
Ile Ser Leu Gln Gly Glu His Met Lys Leu Phe Gln Ser Phe Leu Tyr
420 425 430
Asp Ile
<210> 159
<211> 429
<212> PRT
<213> 葡萄()
<220>
<223> 葡萄羟基肉桂酰辅酶A:莽草酸羟基肉桂酰基转移酶 (VvHCT)
<400> 159
Met Ile Ile Asn Val Arg Glu Ser Thr Met Val Arg Pro Ala Glu Glu
1 5 10 15
Thr Pro Arg Arg Ser Leu Trp Asn Ser Asn Val Asp Leu Val Val Pro
20 25 30
Arg Met His Thr Pro Ser Val Tyr Phe Tyr Arg Pro Ser Gly Ala Ala
35 40 45
Asn Phe Phe Asp Pro Gln Val Met Lys Glu Ala Leu Ser Lys Val Leu
50 55 60
Val Pro Phe Tyr Pro Met Ala Gly Arg Leu Arg Arg Asp Glu Asp Gly
65 70 75 80
Arg Ile Glu Ile Asp Cys Asn Ala Glu Gly Val Leu Phe Val Glu Ala
85 90 95
Asp Thr Gly Ser Val Ile Asp Asp Phe Gly Asp Phe Ala Pro Thr Leu
100 105 110
Glu Leu Arg Gln Leu Ile Pro Thr Val Asp Tyr Ser Gly Asp Ile Gly
115 120 125
Ser Tyr Ser Leu Leu Ile Leu Gln Val Thr His Phe Lys Cys Gly Gly
130 135 140
Val Ser Leu Gly Val Gly Met Gln His His Val Ala Asp Gly Ala Ser
145 150 155 160
Gly Leu His Phe Ile Asn Thr Trp Ser Asp Met Ala Arg Gly Leu Asp
165 170 175
Ile Thr Ile Pro Pro Phe Ile Asp Arg Thr Leu Leu Arg Ala Arg Asp
180 185 190
Pro Pro Gln Pro Ala Phe His His Ile Glu Tyr Gln Pro Pro Pro Gln
195 200 205
Leu Lys Thr Pro Leu Pro Asn Thr Gln Asn Thr Asn Val Cys Ile Phe
210 215 220
Arg Ile Thr Arg Asp Gln Leu Asn Thr Leu Lys Asn Lys Ser Lys Glu
225 230 235 240
Asp Gly Asn Thr Ile Ser Tyr Ser Ser Tyr Val Met Leu Ala Gly His
245 250 255
Val Trp Arg Cys Ala Cys Lys Ala Arg Ser Leu Pro Ala Asp Gln Asp
260 265 270
Ser Lys Met Tyr Ile Ala Thr Asp Gly Arg Ser Arg Leu Arg Pro Ala
275 280 285
Leu Pro Pro Gly Tyr Phe Gly Asn Val Ile Phe Thr Thr Thr Pro Val
290 295 300
Ala Val Ala Gly Glu Leu Met Ser Lys Pro Leu Trp Tyr Ala Ala Ser
305 310 315 320
Lys Ile His Asn Ala Leu Ala Arg Met Asp Asp Glu Tyr Leu Arg Ser
325 330 335
Ala Leu Asp Tyr Leu Glu Ile Gln Pro Asp Leu Thr Ala Leu Val Arg
340 345 350
Gly Ala His Thr Phe Arg Cys Pro Asn Ile Gly Ile Thr Ser Trp Thr
355 360 365
Arg Leu Pro Ile Tyr Asp Ala Asp Phe Gly Trp Gly Arg Pro Ile Phe
370 375 380
Met Gly Pro Gly Gly Ile Ala Leu Glu Gly Leu Ala Phe Ala Leu Pro
385 390 395 400
Ser Pro Thr Asn Asp Gly Ser Leu Ser Ile Ala Ile Ser Leu Gln Glu
405 410 415
Asp His Met Lys Leu Phe Gln Lys Tyr Leu Tyr Glu Ile
420 425
<210> 160
<211> 435
<212> PRT
<213> 红花烟草()
<220>
<223> 烟草羟基肉桂酰辅酶A:莽草酸羟基肉桂酰基转移酶 (NtHCT)
<400> 160
Met Lys Ile Glu Val Lys Glu Ser Thr Met Val Lys Pro Ala Ala Glu
1 5 10 15
Thr Pro Gln Gln Arg Leu Trp Asn Ser Asn Val Asp Leu Val Val Pro
20 25 30
Asn Phe His Thr Pro Ser Val Tyr Phe Tyr Arg Pro Thr Gly Ser Pro
35 40 45
Asn Phe Phe Asp Gly Lys Val Leu Lys Glu Ala Leu Ser Lys Ala Leu
50 55 60
Val Pro Phe Tyr Pro Met Ala Gly Arg Leu Cys Arg Asp Glu Asp Gly
65 70 75 80
Arg Ile Glu Ile Asp Cys Lys Gly Gln Gly Val Leu Phe Val Glu Ala
85 90 95
Glu Ser Asp Gly Val Val Asp Asp Phe Gly Asp Phe Ala Pro Thr Leu
100 105 110
Glu Leu Arg Gln Leu Ile Pro Ala Val Asp Tyr Ser Gln Gly Ile Gln
115 120 125
Ser Tyr Ala Leu Leu Val Leu Gln Ile Thr His Phe Lys Cys Gly Gly
130 135 140
Val Ser Leu Gly Val Gly Met Gln His His Ala Ala Asp Gly Ala Ser
145 150 155 160
Gly Leu His Phe Ile Asn Thr Trp Ser Asp Met Ala Arg Gly Leu Asp
165 170 175
Leu Thr Ile Pro Pro Phe Ile Asp Arg Thr Leu Leu Arg Ala Arg Asp
180 185 190
Pro Pro Gln Pro Gln Phe Pro His Val Glu Tyr Gln Pro Pro Pro Thr
195 200 205
Leu Lys Val Thr Pro Glu Asn Thr Pro Ile Ser Glu Ala Val Pro Glu
210 215 220
Thr Ser Val Ser Ile Phe Lys Leu Thr Arg Asp Gln Ile Asn Thr Leu
225 230 235 240
Lys Ala Lys Ser Lys Glu Asp Gly Asn Thr Val Asn Tyr Ser Ser Tyr
245 250 255
Glu Met Leu Ala Gly His Val Trp Arg Ser Thr Cys Met Ala Arg Gly
260 265 270
Leu Ala His Asp Gln Glu Thr Lys Leu Tyr Ile Ala Thr Asp Gly Arg
275 280 285
Ser Arg Leu Arg Pro Ser Leu Pro Pro Gly Tyr Phe Gly Asn Val Ile
290 295 300
Phe Thr Thr Thr Pro Ile Ala Val Ala Gly Asp Ile Gln Ser Lys Pro
305 310 315 320
Ile Trp Tyr Ala Ala Ser Lys Leu His Asp Ala Leu Ala Arg Met Asp
325 330 335
Asn Asp Tyr Leu Arg Ser Ala Leu Asp Tyr Leu Glu Leu Gln Pro Asp
340 345 350
Leu Lys Ala Leu Val Arg Gly Ala His Thr Phe Lys Cys Pro Asn Leu
355 360 365
Gly Ile Thr Ser Trp Ser Arg Leu Pro Ile His Asp Ala Asp Phe Gly
370 375 380
Trp Gly Arg Pro Ile Phe Met Gly Pro Gly Gly Ile Ala Tyr Glu Gly
385 390 395 400
Leu Ser Phe Ile Leu Pro Ser Pro Thr Asn Asp Gly Ser Gln Ser Val
405 410 415
Ala Ile Ser Leu Gln Ala Glu His Met Lys Leu Phe Glu Lys Phe Leu
420 425 430
Tyr Asp Phe
435
<210> 161
<211> 434
<212> PRT
<213> 红车轴草()
<220>
<223> 三叶草羟基肉桂酰辅酶A:莽草酸羟基肉桂酰基转移酶 (TpHCT)
<400> 161
Met Ile Ile Asn Val Arg Asp Ser Thr Met Val Arg Pro Ser Glu Glu
1 5 10 15
Val Thr Arg Arg Thr Leu Trp Asn Ser Asn Val Asp Leu Val Val Pro
20 25 30
Asn Phe His Thr Pro Ser Val Tyr Phe Tyr Arg Ser Asn Asn Gly Thr
35 40 45
Ser Asn Phe Phe Asp Ala Lys Ile Met Lys Glu Ala Leu Ser Lys Val
50 55 60
Leu Val Pro Phe Tyr Pro Met Ala Gly Arg Leu Arg Arg Asp Glu Asp
65 70 75 80
Gly Arg Val Glu Ile Asp Cys Asp Gly Gln Gly Val Leu Phe Val Glu
85 90 95
Ala Asp Thr Gly Ala Val Ile Asp Asp Phe Gly Asp Phe Ala Pro Thr
100 105 110
Leu Glu Leu Arg Gln Leu Ile Pro Ala Val Asp Tyr Ser Arg Gly Ile
115 120 125
Glu Ser Tyr Pro Leu Leu Val Leu Gln Val Thr Tyr Phe Lys Cys Gly
130 135 140
Gly Val Ser Leu Gly Val Gly Met Gln His His Val Ala Asp Gly Ala
145 150 155 160
Ser Gly Leu His Phe Ile Asn Thr Trp Ser Asp Val Ala Arg Gly Leu
165 170 175
Asp Val Ser Ile Pro Pro Phe Ile Asp Arg Thr Leu Leu Arg Ala Arg
180 185 190
Asp Pro Pro Arg Pro Val Phe Asp His Ile Glu Tyr Lys Pro Pro Pro
195 200 205
Ser Met Lys Thr Thr Gln Gln Ser Thr Lys Pro Gly Ser Asp Gly Ala
210 215 220
Ala Val Ser Ile Phe Lys Leu Thr Arg Glu Gln Leu Ser Thr Leu Lys
225 230 235 240
Ala Lys Ser Lys Glu Ala Gly Asn Thr Ile His Tyr Ser Ser Tyr Glu
245 250 255
Met Leu Ala Gly His Val Trp Arg Ser Val Cys Lys Ala Arg Ser Leu
260 265 270
Pro Asp Asp Gln Glu Thr Lys Leu Tyr Ile Ala Thr Asp Gly Arg Ala
275 280 285
Arg Leu Gln Pro Pro Pro Pro Pro Gly Tyr Phe Gly Asn Val Ile Phe
290 295 300
Thr Thr Thr Pro Ile Ala Ile Ala Gly Asp Leu Met Ser Lys Pro Thr
305 310 315 320
Trp Tyr Ala Ala Ser Arg Ile His Asn Ala Leu Ser Arg Met Asp Asn
325 330 335
Asp Tyr Leu Arg Ser Ala Leu Asp Phe Leu Glu Leu Gln Pro Asp Leu
340 345 350
Lys Ala Leu Val Arg Gly Ala His Thr Phe Lys Cys Pro Asn Leu Gly
355 360 365
Ile Thr Ser Trp Ala Arg Leu Pro Ile His Asp Ala Asp Phe Gly Trp
370 375 380
Gly Arg Pro Ile Phe Met Gly Pro Gly Gly Ile Ala Tyr Glu Gly Leu
385 390 395 400
Ser Phe Ile Ile Pro Ser Ser Thr Asn Asp Gly Ser Leu Ser Val Ala
405 410 415
Ile Ala Leu Gln His Glu His Met Lys Val Phe Lys Glu Phe Leu Tyr
420 425 430
Asp Ile
<210> 162
<211> 436
<212> PRT
<213> 中果咖啡()
<220>
<223> 羟基肉桂酰辅酶A:莽草酸羟基肉桂酰基转移酶 (CcHCT)
<400> 162
Met Lys Ile Glu Val Arg Glu Ser Thr Met Val Arg Pro Ala Glu Glu
1 5 10 15
Thr Pro Arg Ile Asn Leu Trp Asn Ser Asn Val Asp Leu Val Val Pro
20 25 30
Asn Phe His Thr Pro Ser Val Tyr Phe Tyr Arg Pro Asn Gly Ala Ala
35 40 45
Asn Phe Phe Asp Pro Lys Val Met Lys Asp Ala Leu Ser Arg Ala Leu
50 55 60
Val Pro Phe Tyr Pro Met Gly Gly Arg Leu Lys Arg Asp Glu Asp Gly
65 70 75 80
Arg Ile Glu Ile Asp Cys Gln Gly Gln Gly Val Leu Phe Val Glu Ala
85 90 95
Glu Ser Asp Gly Val Ile Asp Asp Phe Gly Asp Phe Ala Pro Thr Leu
100 105 110
Glu Leu Arg Lys Leu Ile Pro Ala Val Asp Tyr Thr Leu Gly Ile Glu
115 120 125
Ser Tyr Ser Leu Leu Val Leu Gln Val Thr Tyr Phe Lys Cys Gly Gly
130 135 140
Val Ser Leu Gly Val Gly Met Gln His His Ala Ala Asp Gly Ala Ser
145 150 155 160
Gly Leu His Phe Ile Asn Thr Trp Ser Asp Leu Ala Arg Gly Leu Asp
165 170 175
Leu Ala Val Pro Pro Phe Ile Asp Arg Thr Leu Leu Arg Ser Arg Asp
180 185 190
Pro Pro Gln Pro Ala Phe Asp His Ile Glu Tyr Gln Pro Ala Pro Pro
195 200 205
Met Lys Thr Ala Pro Thr Pro Thr Pro Thr Asp Asp Glu Ser Val Pro
210 215 220
Glu Thr Thr Val Ser Ile Phe Lys Leu Thr Arg Asp Gln Val Asn Ala
225 230 235 240
Leu Lys Gly Lys Ser Lys Glu Asp Gly Asn Thr Val Asn Tyr Ser Ser
245 250 255
Tyr Glu Met Leu Ser Gly His Val Trp Arg Cys Val Cys Lys Ala Arg
260 265 270
Gly Leu Pro Asp Asp Gln Asp Thr Lys Leu Tyr Ile Ala Thr Asp Gly
275 280 285
Arg Ala Arg Leu Arg Pro Ser Leu Pro Arg Gly Tyr Phe Gly Asn Val
290 295 300
Ile Phe Thr Thr Thr Pro Ile Ala Val Ala Gly Asp Leu Gln Ser Lys
305 310 315 320
Pro Thr Trp Tyr Ala Ala Ser Lys Ile His Asp Ala Leu Ala Arg Met
325 330 335
Asp Asp Asp Tyr Leu Lys Ser Ala Leu Asp Tyr Leu Glu Leu Gln Pro
340 345 350
Asp Leu Lys Ala Leu Val Arg Gly Ala His Thr Phe Lys Cys Pro Asn
355 360 365
Leu Gly Ile Thr Ser Trp Ala Arg Leu Pro Ile His Asp Ala Asp Phe
370 375 380
Gly Trp Gly Arg Pro Ile Phe Met Gly Pro Gly Gly Ile Ala Tyr Glu
385 390 395 400
Gly Leu Ser Phe Val Leu Pro Ser Pro Ile Asn Asp Gly Ser Leu Ser
405 410 415
Ile Val Ile Ser Leu Gln Ala Glu His Met Lys Leu Phe Ser Lys Phe
420 425 430
Leu Tyr Asp Ile
435
<210> 163
<211> 442
<212> PRT
<213> 水稻()
<220>
<223> 稻羟基肉桂酰辅酶A:莽草酸羟基肉桂酰基转移酶 (OsHCT)
<400> 163
Met Ala Ile Thr Val Arg Arg Ser Thr Met Val Arg Pro Ala Trp Glu
1 5 10 15
Thr Pro Arg Val Arg Leu Trp Asn Ser Asn Leu Asp Leu Val Val Pro
20 25 30
Arg Phe His Thr Pro Ser Val Tyr Phe Tyr Arg Arg Gly Pro Glu Gly
35 40 45
Gly Gly Ala Pro Glu Gly Phe Phe Asp Gly Glu Arg Met Arg Arg Ala
50 55 60
Leu Ala Glu Ala Leu Val Pro Phe Tyr Pro Met Ala Gly Arg Leu Ala
65 70 75 80
Arg Asp Glu Asp Gly Arg Val Glu Ile Asp Cys Asn Gly Glu Gly Val
85 90 95
Leu Phe Val Glu Ala Asp Ala Pro Asp Ala Ser Val Asp Asp Tyr Gly
100 105 110
Asp Phe Ala Pro Thr Met Glu Leu Lys Arg Leu Ile Pro Ala Val Asp
115 120 125
Tyr Thr Asp Asp Ile Ser Ser Phe Ser Leu Leu Val Leu Gln Val Thr
130 135 140
Tyr Phe Lys Cys Gly Gly Val Ser Leu Gly Val Gly Met Gln His His
145 150 155 160
Val Ala Asp Gly Met Ser Gly Leu His Phe Ile Asn Ser Trp Ser Asp
165 170 175
Leu Cys Arg Gly Thr Gln Ile Ala Ile Met Pro Phe Ile Asp Arg Thr
180 185 190
Leu Leu Arg Ala Arg Asp Pro Pro Thr Pro Ser Tyr Pro His Val Glu
195 200 205
Tyr Gln Pro Ala Pro Ala Met Leu Ser Ser Val Pro Gln Ser Val Thr
210 215 220
Ala Asn Lys Thr Thr Pro Pro Pro Thr Ala Val Asp Ile Phe Lys Leu
225 230 235 240
Thr Arg Ser Asp Leu Gly Arg Leu Arg Ser Gln Leu Pro Ser Gly Glu
245 250 255
Gly Ala Pro Arg Phe Ser Thr Tyr Ala Val Leu Ala Ala His Val Trp
260 265 270
Arg Cys Val Ser Leu Ala Arg Gly Leu Pro Ser Glu Gln Pro Thr Lys
275 280 285
Leu Tyr Cys Ala Thr Asp Gly Arg Gln Arg Leu Gln Pro Pro Leu Pro
290 295 300
Glu Gly Tyr Phe Gly Asn Val Ile Phe Thr Ala Thr Pro Leu Ala Glu
305 310 315 320
Ala Gly Lys Val Thr Ser Gly Leu Ala Asp Gly Ala Ala Val Ile Gln
325 330 335
Glu Ala Leu Asp Arg Met Asn Asp Ser Tyr Cys Arg Ser Ala Leu Asp
340 345 350
Tyr Leu Glu Leu Gln Pro Asp Leu Ser Ala Leu Val Arg Gly Ala His
355 360 365
Thr Phe Arg Cys Pro Asn Leu Gly Leu Thr Ser Trp Val Arg Leu Pro
370 375 380
Ile His Asp Ala Asp Phe Gly Trp Gly Arg Pro Val Phe Met Gly Pro
385 390 395 400
Gly Gly Ile Ala Tyr Glu Gly Leu Ala Phe Val Leu Pro Ser Ala Asn
405 410 415
Lys Asp Gly Ser Leu Ser Ile Ala Ile Ser Leu Gln Ala Glu His Met
420 425 430
Glu Lys Phe Arg Lys Leu Ile Phe Glu Val
435 440
<210> 164
<211> 442
<212> PRT
<213> 水稻()
<220>
<223> 稻羟基肉桂酰辅酶A:莽草酸羟基肉桂酰基转移酶 (OsHCT3)
<400> 164
Met Lys Ile Asn Val Arg Gly Ser Thr Met Val Arg Pro Ala Glu Glu
1 5 10 15
Thr Pro Arg Val Arg Leu Trp Asn Ser Ser Leu Asp Leu Val Val Pro
20 25 30
Arg Phe His Thr Pro Ser Val Tyr Phe Phe Arg Arg Gly Glu Ala Ala
35 40 45
Ala Ala Glu Gly Gly Ser Tyr Phe Asp Gly Glu Arg Met Arg Arg Ala
50 55 60
Leu Ala Glu Ala Leu Val Pro Phe Tyr Pro Met Ala Gly Arg Leu Ala
65 70 75 80
His Asp Glu Asp Gly Arg Val Glu Ile Asp Cys Asn Gly Glu Gly Val
85 90 95
Leu Phe Val Glu Ala Asp Ala Pro Gly Ala Thr Val Asp Asp Phe Gly
100 105 110
Asp Phe Ala Pro Thr Met Asp Leu Lys Arg Leu Ile Pro Thr Val Asp
115 120 125
Tyr Thr Asp Gly Ile Ser Ser Phe Pro Ile Leu Val Leu Gln Val Thr
130 135 140
His Phe Lys Cys Gly Gly Val Ala Leu Gly Val Gly Met Gln His His
145 150 155 160
Val Ala Asp Gly Phe Ser Gly Leu His Phe Ile Asn Ser Trp Ala Asp
165 170 175
Leu Cys Arg Gly Val Pro Ile Ala Val Met Pro Phe Ile Asp Arg Thr
180 185 190
Leu Val Arg Ala Arg Asp Pro Pro Ala Pro Ser His Pro His Val Glu
195 200 205
Tyr Gln Pro Ala Pro Ala Met Leu Ala Pro Glu Pro Pro Gln Ala Leu
210 215 220
Thr Ala Lys Pro Ala Pro Pro Pro Thr Ala Val Asp Ile Phe Lys Leu
225 230 235 240
Ser Arg Ser Asp Leu Gly Arg Leu Arg Ser Gln Leu Pro Arg Gly Glu
245 250 255
Gly Ala Pro Arg Tyr Ser Thr Tyr Ala Val Leu Ala Ala His Val Trp
260 265 270
Arg Cys Ala Ser Leu Ala Arg Gly Leu Pro Ala Glu Gln Pro Thr Lys
275 280 285
Leu Tyr Cys Ala Thr Asp Gly Arg Gln Arg Leu Gln Pro Ser Leu Pro
290 295 300
Asp Gly Tyr Phe Gly Asn Val Ile Phe Thr Ala Thr Pro Leu Ala Glu
305 310 315 320
Ala Gly Arg Val Thr Gly Ser Leu Ala Asp Gly Ala Ala Thr Ile Gln
325 330 335
Ser Ala Leu Asp Arg Met Asp Ser Gly Tyr Cys Arg Ser Ala Leu Asp
340 345 350
Tyr Leu Glu Leu Gln Pro Asp Leu Ser Ala Leu Val Arg Gly Ala His
355 360 365
Thr Phe Arg Cys Pro Asn Leu Gly Leu Thr Ser Trp Val Arg Leu Pro
370 375 380
Ile His Asp Ala Asp Phe Gly Trp Gly Arg Pro Val Phe Met Gly Pro
385 390 395 400
Gly Gly Ile Ala Tyr Glu Gly Leu Ala Phe Val Leu Pro Ser Ala Ser
405 410 415
Gly Asp Gly Ser Leu Ser Val Ala Ile Ser Leu Gln Ala Glu His Met
420 425 430
Glu Lys Phe Arg Lys Met Ile Phe Asp Phe
435 440
<210> 165
<211> 441
<212> PRT
<213> 两色高梁()
<220>
<223> 高粱羟基肉桂酰辅酶A:莽草酸羟基肉桂酰基转移酶 (SbHCT)
<400> 165
Met Val Ile Thr Val Arg Arg Ser Thr Met Val Arg Pro Ala Arg Glu
1 5 10 15
Thr Pro Arg Gln Arg Leu Trp Asn Ser Asn Leu Asp Leu Val Val Pro
20 25 30
Arg Phe His Thr Pro Ser Val Tyr Phe Tyr Arg Arg Gly Asp Gly Ala
35 40 45
Ala Ala Pro Ala Glu Gly Phe Phe Asp Gly Glu Arg Met Arg Arg Ala
50 55 60
Leu Ala Glu Ala Leu Val Pro Phe Tyr Pro Met Ala Gly Arg Leu Ala
65 70 75 80
Arg Asp Glu Asp Gly Arg Val Glu Ile Asp Cys Asn Gly Glu Gly Val
85 90 95
Leu Phe Val Glu Ala Asp Ala Pro Asp Val Ser Val Asp Asp Tyr Gly
100 105 110
Asp Phe Ala Pro Thr Met Asp Leu Lys Arg Leu Ile Pro Thr Val Asp
115 120 125
Tyr Thr Asp Asp Ile Ser Ala Phe Pro Leu Leu Val Leu Gln Val Thr
130 135 140
His Phe Lys Cys Gly Gly Ile Ser Leu Gly Val Gly Met Gln His His
145 150 155 160
Val Ala Asp Gly Met Ser Gly Leu His Phe Ile Asn Ser Trp Ser Asp
165 170 175
Leu Cys Arg Gly Ala Gln Ile Ser Val Met Pro Phe Ile Asp Arg Thr
180 185 190
Leu Ile Arg Ala Arg Asp Pro Pro Thr Pro Ser Phe His His Ile Glu
195 200 205
Tyr Gln Pro Ala Pro Ala Ile Leu Ser Ser Thr Pro Gln Phe Leu Ala
210 215 220
Ser Lys Ser Lys Pro Pro Ala Thr Ala Val Asp Ile Phe Lys Leu Thr
225 230 235 240
Arg Ser Asp Leu Gly Arg Leu Arg Ser Gln Leu Ala Ala Gly Glu Gly
245 250 255
Ala Pro Arg Phe Ser Thr Tyr Ala Val Leu Ala Ala His Val Trp Lys
260 265 270
Cys Val Ser Leu Ala Arg Ser Leu Pro Pro Glu Gln Pro Thr Lys Leu
275 280 285
Tyr Cys Ala Thr Asp Gly Arg Gln Arg Leu Gln Pro Pro Leu Pro Asp
290 295 300
Gly Tyr Phe Gly Asn Val Ile Phe Thr Ala Thr Pro Leu Ala Glu Ala
305 310 315 320
Gly Lys Val Thr Ser Gly Leu Ala Glu Gly Ala Ala Val Ile Gln Gly
325 330 335
Ala Leu Asp Arg Met Asp Asn Asp Tyr Cys Arg Ser Ala Leu Asp Tyr
340 345 350
Leu Glu Leu Gln Pro Asp Leu Ser Ala Leu Val Arg Gly Ala His Thr
355 360 365
Phe Arg Cys Pro Asn Leu Gly Leu Thr Ser Trp Val Arg Leu Pro Ile
370 375 380
His Asp Ala Asp Phe Gly Trp Gly Arg Pro Val Phe Met Gly Pro Gly
385 390 395 400
Gly Ile Ala Tyr Glu Gly Leu Ala Phe Val Leu Pro Ser Ala Asn Gly
405 410 415
Asp Gly Ser Leu Ser Ile Ala Ile Ser Leu Gln Ala Glu His Met Glu
420 425 430
Lys Phe Arg Lys Leu Ile Cys Glu Val
435 440
<210> 166
<211> 440
<212> PRT
<213> 玉米()
<220>
<223> 玉米羟基肉桂酰辅酶A:莽草酸羟基肉桂酰基转移酶 (ZmHCT)
<400> 166
Met Ala Ile Thr Val Arg Arg Ser Thr Met Val Arg Pro Ala Arg Glu
1 5 10 15
Thr Pro Arg Gln Arg Leu Trp Asn Ser Asn Leu Asp Leu Val Val Pro
20 25 30
Arg Phe His Thr Pro Ser Val Tyr Phe Tyr Arg Arg Asp Gly Pro Val
35 40 45
Pro Asp Ala Glu Gly Phe Phe Asp Gly Glu Arg Met Arg Arg Ala Leu
50 55 60
Ala Glu Ala Leu Val Pro Phe Tyr Pro Met Ala Gly Arg Leu Ala Arg
65 70 75 80
Asp Glu Asp Gly Arg Val Glu Ile Asp Cys Asn Gly Glu Gly Val Leu
85 90 95
Phe Val Glu Ala Asp Ala Pro Asp Ala Ser Val Asp Asp Tyr Gly Asp
100 105 110
Phe Ala Pro Thr Met Glu Leu Lys Arg Leu Ile Pro Thr Val Asp Tyr
115 120 125
Thr Asp Asp Ile Ser Ala Phe Ser Leu Leu Val Leu Gln Val Thr Tyr
130 135 140
Phe Lys Cys Gly Gly Val Ser Leu Gly Val Gly Met Gln His His Ala
145 150 155 160
Ala Asp Gly Met Ser Gly Leu His Phe Ile Asn Ser Trp Ser Asp Leu
165 170 175
Cys Arg Gly Ala Gln Ile Ser Val Met Pro Phe Ile Asp Arg Thr Leu
180 185 190
Leu Arg Ala Arg Asp Pro Pro Thr Pro Ser Phe Gln His Ile Glu Tyr
195 200 205
Gln Ser Ala Pro Ala Met Leu Ser Ser Thr Thr Gln Phe Leu Ala Ser
210 215 220
Lys Ser Lys Pro Pro Ala Thr Ala Val Asp Ile Phe Lys Leu Thr Arg
225 230 235 240
Ser Asp Leu Gly Arg Leu Arg Ser Gln Leu Pro Ala Gly Glu Gly Ala
245 250 255
Pro Arg Phe Ser Thr Tyr Ala Val Leu Ala Ala His Val Trp Lys Cys
260 265 270
Val Ser Leu Ala Arg Ser Leu Pro Pro Glu Gln Pro Thr Lys Leu Tyr
275 280 285
Cys Ala Thr Asp Gly Arg Gln Arg Leu Gln Pro Pro Leu Pro Asp Gly
290 295 300
Tyr Phe Gly Asn Val Ile Phe Thr Ala Thr Pro Leu Ala Glu Ala Gly
305 310 315 320
Lys Val Thr Ser Glu Leu Ala Glu Gly Ala Ala Val Ile Gln Gly Ala
325 330 335
Leu Asp Arg Met Asp Asn Asp Tyr Cys Arg Ser Ala Leu Asp Tyr Leu
340 345 350
Glu Leu Gln Pro Asp Leu Ser Ala Leu Val Arg Gly Ala His Thr Phe
355 360 365
Arg Cys Pro Asn Leu Gly Leu Thr Ser Trp Val Arg Leu Pro Ile His
370 375 380
Asp Ala Asp Phe Gly Trp Gly Arg Pro Val Phe Met Gly Pro Gly Gly
385 390 395 400
Ile Ala Tyr Glu Gly Leu Ala Phe Val Leu Pro Ser Ala Asn Gly Asp
405 410 415
Gly Ser Leu Ser Ile Ala Ile Ser Leu Gln Ala Glu His Met Glu Lys
420 425 430
Phe Arg Lys Leu Ile Gly Glu Ala
435 440
<210> 167
<211> 442
<212> PRT
<213> 玉米()
<220>
<223> 玉米羟基肉桂酰辅酶A:莽草酸羟基肉桂酰基转移酶 (ZmHCT2)
<400> 167
Met Ala Ile Thr Val Arg Arg Ser Thr Met Val Arg Pro Ala Arg Glu
1 5 10 15
Thr Pro Arg Gln Arg Leu Trp Asn Ser Asn Leu Asp Leu Val Val Pro
20 25 30
Arg Phe His Thr Pro Ser Val Tyr Phe Tyr Arg Arg Asp Gly Pro Val
35 40 45
Pro Asp Ala Glu Gly Phe Phe Asp Gly Glu Arg Met Arg Arg Ala Leu
50 55 60
Ala Glu Ala Leu Val Pro Phe Tyr Pro Met Ala Gly Arg Leu Ala Arg
65 70 75 80
Asp Glu Asp Gly Arg Val Glu Ile Asp Cys Asn Gly Glu Gly Val Leu
85 90 95
Phe Val Glu Ala Asp Ala Pro Asp Ala Ser Val Asp Asp Tyr Gly Asp
100 105 110
Phe Ala Pro Thr Met Glu Leu Lys Arg Leu Ile Pro Thr Val Asp Tyr
115 120 125
Thr Asp Asp Ile Ser Ala Phe Ser Leu Leu Val Leu Gln Val Thr Tyr
130 135 140
Phe Lys Cys Gly Gly Val Ser Leu Gly Val Gly Met Gln His His Ala
145 150 155 160
Ala Asp Gly Met Ser Gly Leu His Phe Ile Asn Ser Trp Ser Asp Leu
165 170 175
Cys Arg Gly Ala Gln Ile Ser Val Met Pro Phe Ile Asp Arg Thr Leu
180 185 190
Leu Arg Ala Arg Asp Pro Pro Thr Pro Ser Phe Gln His Ile Glu Tyr
195 200 205
Gln Pro Ala Pro Ala Met Leu Ser Ser Thr Thr Gln Phe Leu Ala Ser
210 215 220
Lys Ser Lys Pro Pro Ala Thr Ala Val Asp Ile Phe Lys Leu Thr Arg
225 230 235 240
Ser Asp Leu Gly Arg Leu Arg Ser Gln Leu Pro Ala Gly Glu Gly Ala
245 250 255
Pro Arg Phe Ser Thr Tyr Ala Val Leu Ala Ala His Val Trp Lys Cys
260 265 270
Val Ser Leu Ala Arg Ser Leu Pro Pro Glu Gln Pro Thr Lys Leu Tyr
275 280 285
Cys Ala Thr Asp Gly Arg Gln Arg Leu Gln Pro Pro Leu Pro Asp Gly
290 295 300
Tyr Phe Gly Asn Val Ile Phe Thr Ala Thr Pro Leu Ala Glu Ala Gly
305 310 315 320
Lys Val Thr Ser Glu Leu Ala Glu Gly Ala Ala Val Ile Gln Gly Ala
325 330 335
Leu Asp Arg Met Asp Asn Asp Tyr Cys Arg Ser Ala Leu Asp Tyr Leu
340 345 350
Asp Leu Gln Pro Asp Leu Ser Ala Leu Val Arg Gly Ala His Thr Phe
355 360 365
Arg Cys Pro Asn Leu Gly Leu Thr Ser Trp Val Arg Leu Pro Ile His
370 375 380
Asp Ala Asp Phe Gly Trp Gly Arg Pro Val Phe Met Gly Pro Gly Gly
385 390 395 400
Ile Ala Tyr Glu Gly Leu Ala Phe Val Leu Pro Ser Ala Asn Gly Asp
405 410 415
Gly Ser Leu Ser Ile Ala Ile Ser Leu Gln Ala Glu His Met Glu Lys
420 425 430
Phe Arg Lys Leu Ile Gly Gly Ala Asn Cys
435 440
<210> 168
<211> 440
<212> PRT
<213> 燕麦()
<220>
<223> 燕麦羟基肉桂酰辅酶A:莽草酸羟基肉桂酰基转移酶 (AsHCT)
<400> 168
Met Lys Ile Thr Val Arg Ser Ser Thr Val Val Val Pro Ala Ala Glu
1 5 10 15
Thr Pro Arg Val Arg Leu Trp Asn Ala Asn Pro Asp Leu Val Val Pro
20 25 30
Arg Phe His Thr Pro Ser Val Tyr Phe Tyr Arg Arg Gly Gly Asp Gly
35 40 45
Asp Ala Cys Cys Tyr Phe Asp Ala Ala Arg Met Arg Arg Ala Leu Ala
50 55 60
Glu Ala Leu Val Pro Phe Tyr Pro Met Ala Gly Arg Leu Ala His Asp
65 70 75 80
Glu Asp Gly Arg Val Glu Ile Asp Cys Asn Ala Glu Gly Val Leu Phe
85 90 95
Val Glu Ala Asp Ala Pro Asp Gly Ala Val Asp Asp Phe Gly Asp Phe
100 105 110
Ala Pro Thr Met Gly Leu Lys Arg Leu Ile Pro Thr Val Asp Phe Thr
115 120 125
Gly Gly Ile Ser Ser Tyr Pro Leu Leu Val Val Gln Val Thr His Phe
130 135 140
Lys Cys Gly Gly Val Ala Leu Gly Ile Gly Met Gln His His Val Ala
145 150 155 160
Asp Gly Phe Ser Gly Leu His Phe Ile Asn Ser Trp Ser Asp Leu Cys
165 170 175
Arg Gly Val Pro Ile Ala Val Met Pro Phe Ile Asp Arg Thr Leu Leu
180 185 190
Arg Ala Arg Asp Pro Pro Val Pro Thr His Pro His Ile Glu Tyr Gln
195 200 205
Pro Ala Pro Ala Met Leu Gly Ser Glu Glu Pro Gln Ala Leu Ala Gly
210 215 220
Lys Pro Glu Ser Pro Pro Thr Ala Val Asp Ile Phe Lys Leu Ser Arg
225 230 235 240
Ser Asp Leu Gly Arg Leu Arg Ala Gln Leu Pro Thr Gly Glu Gly Ala
245 250 255
Pro Arg Phe Ser Thr Tyr Ala Val Leu Gly Ala His Val Trp Arg Cys
260 265 270
Ala Ser Leu Ala Arg Gly Leu Ala Pro Glu Gln Pro Thr Lys Leu Tyr
275 280 285
Cys Ala Thr Asp Gly Arg Gln Arg Leu Thr Pro Thr Leu Pro Asp Gly
290 295 300
Tyr Phe Gly Asn Val Ile Phe Thr Ala Thr Pro Leu Ala Glu Ala Gly
305 310 315 320
Lys Val Thr Gly Ser Leu Ala Asp Gly Ala Thr Thr Ile Gln Asp Ala
325 330 335
Leu Glu Lys Met Asp Asp Glu Tyr Cys His Ser Ala Leu Asp Tyr Leu
340 345 350
Glu Leu Gln Pro Asp Leu Ser Ala Leu Val Arg Gly Ala His Thr Phe
355 360 365
Arg Cys Pro Asn Leu Gly Leu Thr Ser Trp Val Arg Leu Pro Ile His
370 375 380
Asp Ala Asp Phe Gly Trp Gly Arg Pro Val Phe Met Gly Pro Gly Gly
385 390 395 400
Ile Ala Tyr Glu Gly Leu Ala Phe Val Leu Pro Ser Ala Asn Arg Asp
405 410 415
Gly Ser Leu Ser Val Ala Ile Ser Leu Gln Ala Glu His Met Glu Lys
420 425 430
Phe Arg Lys Met Ile Phe Asp Phe
435 440
<210> 169
<211> 449
<212> PRT
<213> 江南卷柏()
<220>
<223> 卷柏羟基肉桂酰辅酶A:莽草酸羟基肉桂酰基转移酶 (SmHCT1)
<400> 169
Met Glu Ile Asn Val Val Arg Glu Thr Met Val Arg Pro Ala Gly Ala
1 5 10 15
Thr Pro Gln Arg Val Leu Trp Asn Ser Asn Val Asp Leu Val Ile Pro
20 25 30
Arg Ile His Thr Ala Ser Val Tyr Phe Tyr Arg Pro Asp Pro Ser Gly
35 40 45
Gly Glu Gly Tyr Phe Glu Gly Gly Val Leu Arg Glu Ala Leu Ala Lys
50 55 60
Ala Leu Val Pro Phe Tyr Pro Met Ala Gly Arg Leu Lys Lys Asp Glu
65 70 75 80
Asn Gly Arg Phe Glu Ile Asn Cys Asn Gly Glu Gly Val Leu Leu Val
85 90 95
Glu Ala Ala Ala Ala Asn Ala Ser Val Asp Glu Tyr Ala Arg Asp Phe
100 105 110
Ala Pro Asp Val Ser Phe Gln Arg Leu Ile Pro Ser Val Asp Tyr Thr
115 120 125
Gln Asp Ile Gly Ser Phe Pro Leu Leu Val Leu Gln Ile Thr Arg Phe
130 135 140
Lys Cys Gly Gly Ala Ser Leu Gly Val Gly Met Glu His His Val Ala
145 150 155 160
Asp Gly Met Ser Gly Ile Thr Phe Ile Asn Thr Trp Ala Ala Met Ala
165 170 175
Arg Gly Glu Asp Pro Lys Ile Val Pro Tyr Ile Asp Arg Thr Leu Leu
180 185 190
Arg Ala Asn Lys Pro Pro Ile Pro Lys Phe Pro His Val Glu Tyr His
195 200 205
Pro Pro Pro Leu Leu Lys His Ala Ala Ala Thr Asn Gly His Ser Asn
210 215 220
Gly Lys Ala Lys Pro Gln Ala Gly Asp Asp Ala Pro Pro Arg Ile Ala
225 230 235 240
Val Gly Leu Phe Lys Phe Thr Lys Glu Gln Leu Gln Ala Leu Lys Ser
245 250 255
Gln Ala Thr Asp Glu Glu Thr Asn Thr Thr Tyr Ser Ser Tyr Glu Met
260 265 270
Leu Ser Gly His Ile Trp Arg Ser Met Cys Leu Ala Arg Gly Leu Asp
275 280 285
Asp Asp Gln Glu Thr Lys Leu Tyr Ile Ala Thr Asp Gly Arg Ala Arg
290 295 300
Val Val Pro Pro Leu Pro Lys His Tyr Phe Gly Asn Val Ile Phe Thr
305 310 315 320
Cys Thr Pro Met Ala Leu Ala Gly Asp Leu Val Ser Arg Pro Leu Tyr
325 330 335
Tyr Ala Ala Ser Val Ile His Asp Ala Val Ser Arg Met Asn Asp Glu
340 345 350
Tyr Leu Arg Ser Ala Leu Asp Tyr Leu Glu Leu Gln Pro Asp Leu Tyr
355 360 365
Lys Leu Val Arg Gly Ala His Thr Phe Arg Ser Pro Asn Leu Gly Ile
370 375 380
Thr Ser Trp Ser Arg Leu Pro Val Tyr Asp Ala Asp Phe Gly Trp Gly
385 390 395 400
Arg Pro Val Phe Met Gly Pro Ala Val Ile Ala Phe Glu Gly Leu Val
405 410 415
Tyr Val Leu Pro Ser Gly Thr Gly Asp Gly Ser Leu Ser Ile Ser Leu
420 425 430
Gly Leu Gln Pro Glu His Met Pro Arg Phe Glu Gln Leu Ile Gly Gln
435 440 445
Ile
<210> 170
<211> 451
<212> PRT
<213> 江南卷柏()
<220>
<223> 卷柏羟基肉桂酰辅酶A:莽草酸羟基肉桂酰基转移酶 (SmHCT2)
<400> 170
Met Glu Ile Asn Val Val Arg Glu Thr Met Val Arg Pro Ala Glu Ala
1 5 10 15
Thr Pro Gln Arg Val Leu Trp Asn Ser Asn Val Asp Leu Val Ile Pro
20 25 30
Arg Ile His Thr Ala Ser Val Tyr Phe Tyr Arg Pro Asp Pro Ser Gly
35 40 45
Gly Glu Gly Tyr Phe Glu Gly Gly Val Leu Arg Glu Ala Leu Ala Lys
50 55 60
Ala Leu Val Pro Phe Tyr Pro Met Ala Gly Arg Leu Lys Lys Asp Glu
65 70 75 80
Asn Gly Arg Phe Glu Ile Asn Cys Asn Gly Glu Gly Val Leu Leu Val
85 90 95
Glu Ala Ala Ala Ala Asn Ala Ser Val Asp Glu Tyr Ala Arg Asp Phe
100 105 110
Ala Pro Asp Val Ser Phe Gln Arg Leu Ile Pro Ser Val Asp Tyr Thr
115 120 125
Gln Asp Ile Gly Ser Phe Pro Leu Leu Val Leu Gln Ile Thr Arg Phe
130 135 140
Lys Cys Gly Gly Ala Ser Leu Gly Val Gly Met Glu His His Val Ala
145 150 155 160
Asp Gly Met Ser Gly Ile Thr Phe Ile Asn Thr Trp Ala Ala Met Ala
165 170 175
Arg Gly Glu Asp Pro Lys Ile Val Pro Tyr Ile Asp Arg Thr Leu Leu
180 185 190
Arg Ala Asn Lys Pro Pro Val Pro Lys Phe Pro His Val Glu Tyr His
195 200 205
Pro Pro Pro Leu Leu Lys Gln Ala Ala Ala Thr Asn Gly His Ser Val
210 215 220
Ser Asn Gly Lys Ala Lys Pro His Thr Gly Asp Asp Ala Pro Pro Arg
225 230 235 240
Ile Ala Val Gly Leu Phe Lys Phe Thr Lys Glu Gln Leu Gln Ala Leu
245 250 255
Lys Ser Gln Ala Thr Asp Glu Glu Thr Asn Thr Thr Tyr Ser Ser Tyr
260 265 270
Glu Met Leu Ser Gly His Ile Trp Arg Ser Met Cys Leu Ala Arg Gly
275 280 285
Leu Asp Asp Asp Gln Glu Thr Lys Leu Tyr Ile Ala Thr Asp Gly Arg
290 295 300
Ala Arg Val Val Pro Pro Leu Pro Lys His Tyr Phe Gly Asn Val Ile
305 310 315 320
Phe Thr Cys Thr Pro Met Ala Leu Ala Gly Asp Leu Val Ser Arg Pro
325 330 335
Leu Tyr Tyr Ala Ala Ser Val Ile His Asn Ala Val Ser Arg Met Asn
340 345 350
Asp Glu Tyr Leu Arg Ser Ala Leu Asp Tyr Leu Glu Leu Gln Pro Asp
355 360 365
Leu Tyr Lys Leu Val Arg Gly Ala His Thr Phe Arg Ser Pro Asn Leu
370 375 380
Gly Ile Thr Ser Trp Ser Arg Leu Pro Val Tyr Asp Ala Asp Phe Gly
385 390 395 400
Trp Gly Arg Pro Val Phe Met Gly Pro Ala Val Ile Ala Phe Glu Gly
405 410 415
Leu Val Tyr Val Leu Pro Ser Gly Thr Gly Asp Gly Ser Leu Ser Ile
420 425 430
Ser Leu Gly Leu Gln Pro Glu His Met Pro Arg Phe Glu Gln Leu Ile
435 440 445
Gly Gln Ile
450
<210> 171
<211> 508
<212> PRT
<213> 人工序列()
<220>
<223> 合成的豆酰基莽草酸3-羟化酶 (C3H) 共有序列（大多数）
<220>
<221> 变体
<222> (6)..(.6)
<223> Xaa = Ile, Thr, Val, Leu, Gly, Ser或Phe
<220>
<221> 变体
<222> (9)..(.9)
<223> Xaa = Ala, Pro, Ser, Ile, Leu或Gly
<220>
<221> 变体
<222> (14)..(.14)
<223> Xaa = Val, Leu, Ile, Phe, Trp, Ala或Pro
<220>
<221> 变体
<222> (16)..(.16)
<223> Xaa = Ser, Ala, Thr, Gly, Phe, Leu, Val或Tyr
<220>
<221> 变体
<222> (57)..(.57)
<223> Xaa = Ser, Ala, Lys, Thr或Arg
<220>
<221> 变体
<222> (156)..(.156)
<223> Xaa = Arg, Lys, Met, Asn, His或Gln
<220>
<221> 变体
<222> (171)..(.171)
<223> Xaa = Arg或Lys
<220>
<221> 变体
<222> (317)..(.317)
<223> Xaa = Lys或Arg
<220>
<221> 变体
<222> (421)..(.421)
<223> Xaa = Met或Ile
<220>
<221> 变体
<222> (483)..(.483)
<223> Xaa = Tyr或Phe
<220>
<221> UNSURE
<222> (6)..(6)
<223> The 'Xaa' at location 6 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (9)..(9)
<223> The 'Xaa' at location 9 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (14)..(14)
<223> The 'Xaa' at location 14 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (16)..(16)
<223> The 'Xaa' at location 16 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (57)..(57)
<223> The 'Xaa' at location 57 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (156)..(156)
<223> The 'Xaa' at location 156 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (171)..(171)
<223> The 'Xaa' at location 171 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (317)..(317)
<223> The 'Xaa' at location 317 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (421)..(421)
<223> The 'Xaa' at location 421 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (483)..(483)
<223> The 'Xaa' at location 483 stands for Gln, Arg, Pro, or Leu.
<400> 171
Met Ala Leu Leu Leu Xaa Ala Ile Xaa Leu Val Ala Ile Xaa Leu Xaa
1 5 10 15
Tyr Lys Leu Tyr Gln Arg Leu Arg Phe Lys Leu Pro Pro Gly Pro Arg
20 25 30
Pro Trp Pro Val Val Gly Asn Leu Tyr Asp Ile Lys Pro Val Arg Phe
35 40 45
Arg Cys Phe Ala Glu Trp Ala Gln Xaa Tyr Gly Pro Ile Ile Ser Val
50 55 60
Trp Phe Gly Ser Thr Leu Asn Val Val Val Ser Asn Ser Glu Leu Ala
65 70 75 80
Lys Glu Val Leu Lys Glu Asn Asp Gln Gln Leu Ala Asp Arg Pro Arg
85 90 95
Ser Arg Ser Ala Ala Lys Phe Ser Arg Asn Gly Gln Asp Leu Ile Trp
100 105 110
Ala Asp Tyr Gly Pro His Tyr Val Lys Val Arg Lys Val Cys Thr Leu
115 120 125
Glu Leu Phe Thr Pro Lys Arg Leu Glu Ala Leu Arg Pro Ile Arg Glu
130 135 140
Asp Glu Val Thr Ala Met Val Glu Ser Ile Phe Xaa Asp Cys Thr Asn
145 150 155 160
Pro Glu Asn Glu Gly Lys Pro Leu Leu Val Xaa Lys Tyr Leu Gly Ala
165 170 175
Val Ala Phe Asn Asn Ile Thr Arg Leu Ala Phe Gly Lys Arg Phe Val
180 185 190
Asn Ala Glu Gly Val Met Asp Glu Gln Gly Val Glu Phe Lys Ala Ile
195 200 205
Val Ala Asn Gly Leu Lys Leu Gly Ala Ser Leu Ala Met Ala Glu His
210 215 220
Ile Pro Trp Leu Arg Trp Met Phe Pro Leu Glu Glu Glu Ala Phe Ala
225 230 235 240
Lys His Gly Ala Arg Arg Asp Arg Leu Thr Arg Ala Ile Met Glu Glu
245 250 255
His Thr Leu Ala Arg Gln Lys Ser Gly Gly Ala Lys Gln His Phe Val
260 265 270
Asp Ala Leu Leu Thr Leu Gln Asp Lys Tyr Asp Leu Ser Glu Asp Thr
275 280 285
Ile Ile Gly Leu Leu Trp Asp Met Ile Thr Ala Gly Met Asp Thr Thr
290 295 300
Ala Ile Ser Val Glu Trp Ala Met Ala Glu Leu Ile Xaa Asn Pro Arg
305 310 315 320
Val Gln Gln Lys Ala Gln Glu Glu Leu Asp Arg Val Ile Gly Arg Asp
325 330 335
Arg Val Met Thr Glu Thr Asp Phe Ser Asn Leu Pro Tyr Leu Gln Cys
340 345 350
Val Ala Lys Glu Ala Leu Arg Leu His Pro Pro Thr Pro Leu Met Leu
355 360 365
Pro His Lys Ala Asn Ala Asn Val Lys Ile Gly Gly Tyr Asp Ile Pro
370 375 380
Lys Gly Ser Asn Val His Val Asn Val Trp Ala Val Ala Arg Asp Pro
385 390 395 400
Ala Val Trp Lys Asn Pro Leu Glu Phe Arg Pro Glu Arg Phe Leu Glu
405 410 415
Glu Asp Val Asp Xaa Lys Gly His Asp Phe Arg Leu Leu Pro Phe Gly
420 425 430
Ala Gly Arg Arg Val Cys Pro Gly Ala Gln Leu Gly Ile Asn Leu Val
435 440 445
Thr Ser Met Leu Gly His Leu Leu His His Phe Glu Trp Ala Pro Pro
450 455 460
Glu Gly Val Lys Pro Glu Asp Ile Asp Met Thr Glu Asn Pro Gly Leu
465 470 475 480
Val Thr Xaa Met Arg Thr Pro Leu Gln Ala Val Ala Thr Pro Arg Leu
485 490 495
Pro Ser Glu Leu Tyr Lys Arg Val Pro Val Asp Met
500 505
<210> 172
<211> 509
<212> PRT
<213> 蓝桉()
<220>
<223> 豆酰基莽草酸3-羟化酶 (EgC3H)
<400> 172
Met Ala Leu Pro Leu Ile Leu Leu Ser Ile Pro Leu Leu Phe Leu Leu
1 5 10 15
Leu Ala His Gln Leu Tyr Gln Arg Leu Arg Phe Lys Leu Pro Pro Gly
20 25 30
Pro Arg Ala Trp Pro Val Val Gly Asn Leu Tyr Asp Ile Lys Pro Val
35 40 45
Arg Phe Arg Cys Phe Ala Glu Trp Ser Gln Ala Tyr Gly Pro Ile Ile
50 55 60
Ser Val Trp Phe Gly Ser Thr Leu Asn Val Val Val Ser Ser Ser Glu
65 70 75 80
Leu Ala Lys Glu Val Leu Lys Glu Asn Asp Gln Gln Leu Ala Asp Arg
85 90 95
His Arg Ser Arg Ser Ala Ala Lys Phe Ser Arg Asp Gly Gln Asp Leu
100 105 110
Ile Trp Ala Asp Tyr Gly Pro His Tyr Val Lys Val Arg Lys Val Cys
115 120 125
Thr Leu Glu Leu Phe Thr Pro Lys Arg Leu Glu Ala Leu Arg Pro Ile
130 135 140
Arg Glu Asp Glu Val Thr Ala Met Val Glu Ser Ile Phe Lys Asp Cys
145 150 155 160
Thr Asn Pro Asp Asn Ser Gly Lys Thr Leu Leu Val Lys Lys Tyr Leu
165 170 175
Gly Ala Val Ala Phe Asn Asn Ile Thr Arg Leu Ala Phe Gly Lys Arg
180 185 190
Phe Met Asn Ala Glu Gly Val Ile Asp Glu Gln Gly Leu Glu Phe Lys
195 200 205
Ala Ile Val Ser Asn Gly Leu Lys Leu Gly Ala Ser Leu Ala Met Ala
210 215 220
Glu His Ile Pro Trp Leu Arg Trp Met Phe Pro Leu Glu Glu Glu Ala
225 230 235 240
Phe Ala Lys His Ser Ala Arg Arg Asp Arg Leu Thr Arg Ala Ile Met
245 250 255
Glu Glu His Thr Val Ala Arg Gln Lys Ser Gly Ala Lys Gln His Phe
260 265 270
Val Asp Ala Leu Leu Thr Leu Lys Asp Lys Tyr Asp Leu Ser Glu Asp
275 280 285
Thr Ile Ile Gly Leu Leu Trp Asp Met Ile Thr Ala Gly Met Asp Thr
290 295 300
Thr Ala Ile Ser Val Glu Trp Ala Met Ala Glu Leu Ile Lys Asn Pro
305 310 315 320
Arg Val Gln Gln Lys Ala Gln Glu Glu Leu Asp Arg Val Val Gly Phe
325 330 335
Glu Arg Val Val Thr Glu Pro Asp Phe Ser Asn Leu Pro Tyr Leu Gln
340 345 350
Cys Ile Ala Lys Glu Ala Leu Arg Leu His Pro Pro Thr Pro Leu Met
355 360 365
Leu Pro His Arg Ser Asn Ser His Val Lys Ile Gly Gly Tyr Asp Ile
370 375 380
Pro Lys Gly Ser Asn Val His Val Asn Val Trp Ala Ile Ala Arg Asp
385 390 395 400
Pro Ala Val Trp Asn Ser Pro Leu Glu Phe Arg Pro Glu Arg Phe Leu
405 410 415
Glu Glu Asp Val Asp Met Lys Gly His Asp Phe Arg Leu Leu Pro Phe
420 425 430
Gly Ala Gly Arg Arg Val Cys Pro Gly Ala Gln Leu Gly Ile Asn Leu
435 440 445
Val Thr Ser Met Leu Gly His Leu Leu His His Phe Val Trp Thr Pro
450 455 460
Pro Gln Gly Thr Lys Pro Glu Glu Ile Asp Met Ser Glu Asn Pro Gly
465 470 475 480
Leu Val Thr Tyr Met Ser Thr Pro Val Gln Ala Val Ala Thr Pro Arg
485 490 495
Leu Pro Ser Glu Leu Tyr Lys Arg Val Pro Tyr Glu Met
500 505
<210> 173
<211> 511
<212> PRT
<213> 蓖麻()
<220>
<223> 豆酰基莽草酸3-羟化酶 (RcC3H)
<400> 173
Met Ala Phe Pro Leu Ile Val Thr Ile Pro Ile Ser Ile Leu Leu Leu
1 5 10 15
Ile Leu Thr Tyr Lys Leu Tyr Tyr Arg Leu Arg Phe Lys Leu Pro Pro
20 25 30
Gly Pro Arg Pro Trp Pro Ile Val Gly Asn Leu Tyr Asp Ile Lys Pro
35 40 45
Val Arg Phe Arg Cys Phe Ala Glu Trp Ala Gln Ala Tyr Gly Pro Ile
50 55 60
Ile Ser Val Trp Phe Gly Ser Thr Leu Asn Val Ile Val Ser Asn Thr
65 70 75 80
Glu Leu Ala Lys Glu Val Leu Lys Glu His Asp Gln Gln Leu Ala Asp
85 90 95
Arg His Arg Ser Arg Ser Ala Ala Lys Phe Ser Arg Asp Gly Lys Asp
100 105 110
Leu Ile Trp Ala Asp Tyr Gly Pro His Tyr Val Lys Val Arg Lys Val
115 120 125
Cys Thr Leu Glu Leu Phe Thr Pro Lys Arg Leu Asp Ala Leu Arg Pro
130 135 140
Ile Arg Glu Asp Glu Val Thr Ala Met Val Glu Ser Ile Phe Met Asp
145 150 155 160
Cys Thr Asn Pro Glu Asn Asn Gly Lys Ser Val Leu Val Lys Lys Tyr
165 170 175
Leu Gly Ala Val Ala Phe Asn Asn Ile Thr Arg Leu Ala Phe Gly Lys
180 185 190
Arg Phe Val Asn Ala Glu Gly Ile Met Asp Glu Gln Gly Lys Glu Phe
195 200 205
Lys Ala Ile Val Ala Asn Gly Leu Lys Leu Gly Ala Ser Leu Ala Met
210 215 220
Ala Glu His Ile Pro Trp Leu Arg Trp Met Phe Pro Leu Glu Glu Asp
225 230 235 240
Ala Phe Ala Lys His Gly Ala Arg Arg Asp Arg Leu Thr Arg Ala Ile
245 250 255
Met Glu Glu His Thr Leu Ala Arg Gln Lys Ser Gly Gly Ala Lys Gln
260 265 270
His Phe Val Asp Ala Leu Leu Thr Leu Gln Gln Lys Tyr Asp Leu Ser
275 280 285
Glu Asp Thr Ile Ile Gly Leu Leu Trp Asp Met Ile Thr Ala Gly Met
290 295 300
Asp Thr Thr Ala Ile Thr Val Glu Trp Ala Met Ala Glu Leu Ile Lys
305 310 315 320
Asn Pro Arg Val Gln Gln Lys Val Gln Glu Glu Leu Asp Arg Val Ile
325 330 335
Gly Phe Glu Arg Val Leu Thr Glu Ala Asp Phe Ser Ser Leu Pro Tyr
340 345 350
Leu Gln Cys Ile Ala Lys Glu Gly Leu Arg Leu His Pro Pro Thr Pro
355 360 365
Leu Met Leu Pro His Arg Ala Asn Ser Asn Val Lys Ile Gly Gly Tyr
370 375 380
Asp Ile Pro Lys Gly Ser Asn Val His Val Asn Val Trp Ala Val Ala
385 390 395 400
Arg Asp Pro Ala Ile Trp Lys Ser Pro Glu Glu Phe Arg Pro Glu Arg
405 410 415
Phe Leu Glu Glu Asp Val Asp Met Lys Gly His Asp Phe Arg Leu Leu
420 425 430
Pro Phe Gly Ala Gly Arg Arg Ile Cys Pro Gly Ala Gln Leu Gly Ile
435 440 445
Asn Leu Val Thr Ser Met Leu Gly His Leu Leu His His Phe Arg Trp
450 455 460
Thr Pro Pro Glu Gly Val Lys Pro Glu Glu Ile Asp Met Ser Glu Asn
465 470 475 480
Pro Gly Leu Val Thr Tyr Met Arg Thr Pro Leu Gln Ala Val Ala Thr
485 490 495
Pro Arg Leu Pro Ser Glu Leu Tyr Lys Arg Val Ala Val Asp Met
500 505 510
<210> 174
<211> 508
<212> PRT
<213> 葡萄()
<220>
<223> 葡萄豆酰基莽草酸3-羟化酶 (VvC3H)
<400> 174
Met Ala Leu Leu Leu Val Leu Ile Ser Ile Thr Ala Ile Phe Leu Ala
1 5 10 15
Tyr Lys Leu Tyr Gln Arg Leu Arg Phe Lys Leu Pro Pro Gly Pro His
20 25 30
Pro Trp Pro Ile Val Gly Asn Leu Tyr Asp Ile Lys Pro Val Arg Phe
35 40 45
Arg Cys Phe Ala Glu Trp Ser Gln Ala Tyr Gly Pro Ile Ile Ser Val
50 55 60
Trp Phe Gly Ser Thr Leu Asn Val Ile Val Ser Asn Ser Glu Leu Ala
65 70 75 80
Lys Glu Ala Leu Lys Glu Lys Asp Gln Gln Leu Ala Asp Arg His Arg
85 90 95
Ser Arg Ser Ala Ala Lys Phe Ser Arg Asp Gly Lys Asp Leu Ile Trp
100 105 110
Ala Asp Tyr Gly Pro His Tyr Val Lys Val Arg Lys Val Cys Thr Leu
115 120 125
Glu Leu Phe Ser Pro Lys Arg Leu Glu Ala Leu Arg Pro Ile Arg Glu
130 135 140
Asp Glu Val Thr Ala Met Val Glu Ser Ile Phe Lys Asp Val Thr Asn
145 150 155 160
Pro Glu Asn Leu Gly Lys Ser Ile Leu Leu Lys Lys Tyr Leu Gly Ala
165 170 175
Val Ala Phe Asn Asn Ile Thr Arg Leu Ala Phe Gly Lys Arg Phe Met
180 185 190
Asn Ser Glu Gly Val Ile Asp Glu Gln Gly Leu Glu Phe Lys Ala Ile
195 200 205
Val Ala Asn Gly Leu Lys Leu Gly Ala Ser Leu Ala Met Ala Glu His
210 215 220
Ile Pro Trp Leu Arg Trp Met Phe Pro Leu Glu Glu Glu Ala Phe Ala
225 230 235 240
Lys His Gly Ala Arg Arg Asp Arg Leu Thr Arg Ala Ile Met Glu Glu
245 250 255
His Thr Leu Ala Arg Glu Lys Ser Gly Gly Ala Lys Gln His Phe Val
260 265 270
Asp Ala Leu Leu Thr Leu Gln Asp Lys Tyr Asp Leu Ser Glu Asp Thr
275 280 285
Ile Ile Gly Leu Leu Trp Asp Met Ile Thr Ala Gly Met Asp Thr Thr
290 295 300
Ala Ile Ser Val Glu Trp Ala Met Ala Glu Leu Ile Lys Asn Pro Arg
305 310 315 320
Val Gln Gln Lys Val Gln Ala Glu Leu Asp His Val Ile Gly Leu Asp
325 330 335
Arg Val Met Ser Glu Ser Asp Phe Ser Asn Leu Pro Tyr Leu Gln Ser
340 345 350
Val Ala Lys Glu Ala Leu Arg Leu His Pro Pro Thr Pro Leu Met Leu
355 360 365
Pro His Arg Ala Asn Ala Asn Val Lys Ile Gly Gly Tyr Asp Ile Pro
370 375 380
Lys Gly Ser Asn Val His Val Asn Val Trp Ala Val Ala Arg Asp Pro
385 390 395 400
Ala Val Trp Lys Asp Pro Glu Glu Phe Arg Pro Glu Arg Phe Leu Glu
405 410 415
Glu Asp Val Asp Met Lys Gly His Asp Phe Arg Leu Leu Pro Phe Gly
420 425 430
Ala Gly Arg Arg Val Cys Pro Gly Ala Gln Leu Gly Ile Asn Leu Val
435 440 445
Thr Ser Met Leu Gly His Leu Leu His His Phe Asn Trp Ala Pro Pro
450 455 460
Glu Gly Val Asn Pro Glu Asp Leu Asp Met Ser Glu Asn Pro Gly Leu
465 470 475 480
Val Ser Tyr Met Arg Thr Pro Leu Gln Ala Ile Pro Thr Ser Arg Leu
485 490 495
Pro Ala Ser Leu Tyr Lys Arg Met Ala Val Asp Ile
500 505
<210> 175
<211> 509
<212> PRT
<213> 大豆()
<220>
<223> 大豆豆酰基莽草酸3-羟化酶 (GmC3H)
<400> 175
Met Ala Leu Leu Leu Ile Ile Pro Ile Ser Leu Val Thr Leu Trp Leu
1 5 10 15
Gly Tyr Thr Leu Tyr Gln Arg Leu Arg Phe Lys Leu Pro Pro Gly Pro
20 25 30
Arg Pro Trp Pro Val Val Gly Asn Leu Tyr Asp Ile Lys Pro Val Arg
35 40 45
Phe Arg Cys Phe Ala Glu Trp Ala Gln Ser Tyr Gly Pro Ile Ile Ser
50 55 60
Val Trp Phe Gly Ser Thr Leu Asn Val Ile Val Ser Asn Ser Glu Leu
65 70 75 80
Ala Lys Glu Val Leu Lys Glu His Asp Gln Leu Leu Ala Asp Arg His
85 90 95
Arg Ser Arg Ser Ala Ala Lys Phe Ser Arg Asp Gly Lys Asp Leu Ile
100 105 110
Trp Ala Asp Tyr Gly Pro His Tyr Val Lys Val Arg Lys Val Cys Thr
115 120 125
Leu Glu Leu Phe Ser Pro Lys Arg Leu Glu Ala Leu Arg Pro Ile Arg
130 135 140
Glu Asp Glu Val Thr Ser Met Val Asp Ser Val Tyr Asn His Cys Thr
145 150 155 160
Ser Thr Glu Asn Leu Gly Lys Gly Ile Leu Leu Arg Lys His Leu Gly
165 170 175
Val Val Ala Phe Asn Asn Ile Thr Arg Leu Ala Phe Gly Lys Arg Phe
180 185 190
Val Asn Ser Glu Gly Val Met Asp Glu Gln Gly Val Glu Phe Lys Ala
195 200 205
Ile Val Glu Asn Gly Leu Lys Leu Gly Ala Ser Leu Ala Met Ala Glu
210 215 220
His Ile Pro Trp Leu Arg Trp Met Phe Pro Leu Glu Glu Gly Ala Phe
225 230 235 240
Ala Lys His Gly Ala Arg Arg Asp Arg Leu Thr Arg Ala Ile Met Ala
245 250 255
Glu His Thr Glu Ala Arg Lys Lys Ser Gly Gly Ala Lys Gln His Phe
260 265 270
Val Asp Ala Leu Leu Thr Leu Gln Asp Lys Tyr Asp Leu Ser Glu Asp
275 280 285
Thr Ile Ile Gly Leu Leu Trp Asp Met Ile Thr Ala Gly Met Asp Thr
290 295 300
Thr Ala Ile Ser Val Glu Trp Ala Met Ala Glu Leu Ile Arg Asn Pro
305 310 315 320
Arg Val Gln Gln Lys Val Gln Glu Glu Leu Asp Arg Val Ile Gly Leu
325 330 335
Glu Arg Val Met Thr Glu Ala Asp Phe Ser Asn Leu Pro Tyr Leu Gln
340 345 350
Cys Val Thr Lys Glu Ala Met Arg Leu His Pro Pro Thr Pro Leu Met
355 360 365
Leu Pro His Arg Ala Asn Ala Asn Val Lys Val Gly Gly Tyr Asp Ile
370 375 380
Pro Lys Gly Ser Asn Val His Val Asn Val Trp Ala Val Ala Arg Asp
385 390 395 400
Pro Ala Val Trp Lys Asp Pro Leu Glu Phe Arg Pro Glu Arg Phe Leu
405 410 415
Glu Glu Asp Val Asp Met Lys Gly His Asp Phe Arg Leu Leu Pro Phe
420 425 430
Gly Ser Gly Arg Arg Val Cys Pro Gly Ala Gln Leu Gly Ile Asn Leu
435 440 445
Ala Ala Ser Met Leu Gly His Leu Leu His His Phe Cys Trp Thr Pro
450 455 460
Pro Glu Gly Met Lys Pro Glu Glu Ile Asp Met Gly Glu Asn Pro Gly
465 470 475 480
Leu Val Thr Tyr Met Arg Thr Pro Ile Gln Ala Val Val Ser Pro Arg
485 490 495
Leu Pro Ser His Leu Tyr Lys Arg Val Pro Ala Glu Ile
500 505
<210> 176
<211> 509
<212> PRT
<213> 红车轴草()
<220>
<223> 三叶草豆酰基莽草酸3-羟化酶 (TpC3H)
<400> 176
Met Ala Leu Phe Leu Thr Ile Pro Leu Ser Leu Ile Thr Ile Phe Leu
1 5 10 15
Phe Tyr Thr Leu Phe Gln Arg Leu Arg Phe Lys Leu Pro Pro Gly Pro
20 25 30
Arg Pro Trp Pro Val Val Gly Asn Leu Tyr Asp Ile Lys Pro Val Arg
35 40 45
Phe Arg Cys Phe Ala Glu Trp Ala Gln Ser Tyr Gly Pro Ile Ile Ser
50 55 60
Val Trp Phe Gly Ser Thr Leu Asn Val Ile Val Ser Asn Ser Glu Leu
65 70 75 80
Ala Lys Glu Val Leu Lys Glu Lys Asp Gln Gln Leu Ala Asp Arg His
85 90 95
Arg Ser Arg Ser Ala Ala Lys Phe Ser Arg Asp Gly Gln Asp Leu Ile
100 105 110
Trp Ala Asp Tyr Gly Pro His Tyr Val Lys Val Arg Lys Val Cys Thr
115 120 125
Leu Glu Leu Phe Ser Pro Lys Arg Ile Glu Ala Leu Arg Pro Ile Arg
130 135 140
Glu Asp Glu Val Thr Ala Met Val Glu Ser Ile Phe Asn Asp Ser Thr
145 150 155 160
Asn Ser Glu Asn Leu Gly Lys Gly Ile Leu Met Arg Lys Tyr Ile Gly
165 170 175
Ala Val Ala Phe Asn Asn Ile Thr Arg Leu Ala Phe Gly Lys Arg Phe
180 185 190
Val Asn Ser Glu Gly Val Met Asp Glu Gln Gly Val Glu Phe Lys Ala
195 200 205
Ile Val Ala Asn Gly Leu Lys Leu Gly Ala Ser Leu Ala Met Ala Glu
210 215 220
His Ile Pro Trp Leu Arg Trp Met Phe Pro Leu Glu Glu Glu Ala Phe
225 230 235 240
Ala Lys His Gly Ala Arg Arg Asp Arg Leu Thr Arg Ala Ile Met Asp
245 250 255
Glu His Thr Gln Ala Arg Gln Lys Ser Gly Gly Ala Lys Gln His Phe
260 265 270
Val Asp Ala Leu Leu Thr Leu Gln Asp Lys Tyr Asp Leu Ser Glu Asp
275 280 285
Thr Ile Ile Gly Leu Leu Trp Asp Met Ile Thr Ala Gly Met Asp Thr
290 295 300
Thr Ala Ile Ser Val Glu Trp Ala Met Ala Glu Leu Ile Lys Asn Pro
305 310 315 320
Arg Val Gln Lys Lys Ala Gln Glu Glu Leu Asp Lys Val Ile Gly Phe
325 330 335
Glu Arg Val Met Thr Glu Thr Asp Phe Ser Ser Leu Pro Tyr Leu Gln
340 345 350
Ser Val Ala Lys Glu Ala Leu Arg Leu His Pro Pro Thr Pro Leu Met
355 360 365
Leu Pro His Arg Ala Asn Ala Asn Val Lys Ile Gly Gly Tyr Asp Ile
370 375 380
Pro Lys Gly Ser Asn Val His Val Asn Val Trp Ala Val Ala Arg Asp
385 390 395 400
Pro Ala Val Trp Lys Asn Ala Thr Glu Phe Arg Pro Glu Arg Phe Leu
405 410 415
Glu Glu Asp Val Asp Met Lys Gly His Asp Phe Arg Leu Leu Pro Phe
420 425 430
Gly Ala Gly Arg Arg Val Cys Pro Gly Ala Gln Leu Gly Ile Asn Met
435 440 445
Val Thr Ser Met Leu Gly His Leu Leu His His Phe Cys Trp Ala Ala
450 455 460
Pro Glu Gly Val Asn Pro Glu Asp Ile Asp Met Thr Glu Asn Pro Gly
465 470 475 480
Met Val Thr Tyr Met Arg Thr Pro Leu Gln Val Val Ala Ser Pro Arg
485 490 495
Leu Pro Ser Glu Leu Tyr Lys Arg Val Pro Ala Asp Ile
500 505
<210> 177
<211> 509
<212> PRT
<213> 蒺藜状苜蓿()
<220>
<223> 豆酰基莽草酸3-羟化酶 (MtC3H)
<400> 177
Met Ala Leu Phe Leu Thr Ile Pro Leu Ser Phe Ile Ala Ile Phe Leu
1 5 10 15
Phe Tyr Thr Leu Phe Gln Arg Leu Arg Phe Lys Leu Pro Pro Gly Pro
20 25 30
Arg Pro Trp Pro Val Val Gly Asn Leu Tyr Asp Ile Lys Pro Val Arg
35 40 45
Phe Arg Cys Phe Ala Glu Trp Ala Gln Ser Tyr Gly Pro Ile Ile Ser
50 55 60
Val Trp Phe Gly Ser Thr Leu Asn Val Ile Val Ser Asn Ser Lys Leu
65 70 75 80
Ala Lys Glu Val Leu Lys Glu Asn Asp Gln Gln Leu Ala Asp Arg His
85 90 95
Arg Ser Arg Ser Ala Ala Lys Phe Ser Arg Asp Gly Gln Asp Leu Ile
100 105 110
Trp Ala Asp Tyr Gly Pro His Tyr Val Lys Val Arg Lys Val Cys Thr
115 120 125
Leu Glu Leu Phe Ser Pro Lys Arg Ile Glu Ala Leu Arg Pro Ile Arg
130 135 140
Glu Asp Glu Val Thr Ala Met Val Glu Ser Ile Phe Asn Asp Ser Thr
145 150 155 160
Asn Ser Glu Asn Leu Gly Lys Gly Ile Leu Met Arg Lys Tyr Ile Gly
165 170 175
Ala Val Ala Phe Asn Asn Ile Thr Arg Leu Ala Phe Gly Lys Arg Phe
180 185 190
Val Asn Ser Glu Gly Val Met Asp Glu Gln Gly Val Glu Phe Lys Ala
195 200 205
Ile Val Ala Asn Gly Leu Lys Leu Gly Ala Ser Leu Ala Met Ala Glu
210 215 220
His Ile Pro Trp Leu Arg Trp Met Phe Pro Leu Glu Glu Glu Ala Phe
225 230 235 240
Ala Lys His Gly Ala Arg Arg Asp Arg Leu Asn Arg Ala Ile Met Glu
245 250 255
Glu His Thr Gln Ala Arg Gln Lys Ser Gly Gly Ala Lys Gln His Phe
260 265 270
Val Asp Ala Leu Leu Thr Leu Gln Glu Lys Tyr Asp Leu Ser Glu Asp
275 280 285
Thr Ile Ile Gly Leu Leu Trp Asp Met Ile Thr Ala Gly Met Asp Thr
290 295 300
Thr Ala Ile Ser Val Glu Trp Ala Met Ala Glu Leu Ile Lys Asn Pro
305 310 315 320
Arg Val Gln Gln Lys Ala Gln Glu Glu Leu Asp Lys Val Ile Gly Phe
325 330 335
Glu Arg Val Met Thr Glu Thr Asp Phe Ser Ser Leu Pro Tyr Leu Gln
340 345 350
Cys Val Ala Lys Glu Ala Leu Arg Leu His Pro Pro Thr Pro Leu Met
355 360 365
Leu Pro His Arg Ala Asn Thr Asn Val Lys Ile Gly Gly Tyr Asp Ile
370 375 380
Pro Lys Gly Ser Asn Val His Val Asn Val Trp Ala Val Ala Arg Asp
385 390 395 400
Pro Ala Val Trp Lys Asp Ala Thr Glu Phe Arg Pro Glu Arg Phe Leu
405 410 415
Glu Glu Asp Val Asp Met Lys Gly His Asp Phe Arg Leu Leu Pro Phe
420 425 430
Gly Ala Gly Arg Arg Val Cys Pro Gly Ala Gln Leu Gly Ile Asn Met
435 440 445
Val Thr Ser Met Leu Gly His Leu Leu His His Phe Cys Trp Ala Pro
450 455 460
Pro Glu Gly Val Asn Pro Ala Glu Ile Asp Met Ala Glu Asn Pro Gly
465 470 475 480
Met Val Thr Tyr Met Arg Thr Pro Leu Gln Val Val Ala Ser Pro Arg
485 490 495
Leu Pro Ser Glu Leu Tyr Lys Arg Val Thr Ala Asp Ile
500 505
<210> 178
<211> 508
<212> PRT
<213> 中果咖啡()
<220>
<223> 豆酰基莽草酸3-羟化酶 (CcC3H)
<400> 178
Met Ala Leu Phe Leu Leu Leu Leu Thr Phe Ile Phe Ile Leu Pro Pro
1 5 10 15
Tyr Tyr Leu Tyr Gln Lys Leu Arg Phe Lys Leu Pro Pro Gly Pro Arg
20 25 30
Pro Leu Pro Val Val Gly Asn Leu Tyr Asp Ile Lys Pro Val Arg Phe
35 40 45
Arg Cys Phe Ala Asp Trp Ser Arg Ala Tyr Gly Pro Ile Ile Ser Val
50 55 60
Trp Phe Gly Ser Thr Leu Asn Val Val Val Ser Asn Ala Glu Leu Ala
65 70 75 80
Lys Glu Val Leu Lys Glu Asn Asp Gln Gln Leu Ser Asp Arg His Arg
85 90 95
Ser Arg Ser Ala Ala Lys Phe Ser Arg Glu Gly Gln Asp Leu Ile Trp
100 105 110
Ala Asp Tyr Gly Pro His Tyr Val Lys Val Arg Lys Val Cys Thr Leu
115 120 125
Glu Leu Phe Ser Pro Lys Arg Leu Glu Ala Leu Lys Pro Ile Arg Glu
130 135 140
Asp Glu Val Thr Ala Met Val Glu Ser Ile Tyr Lys Asp Cys Thr Leu
145 150 155 160
Arg Glu Gly Ser Gly Gln Ser Leu Leu Val Lys Lys Tyr Leu Gly Thr
165 170 175
Val Ala Phe Asn Asn Ile Thr Arg Leu Ala Phe Gly Lys Arg Phe Val
180 185 190
Asn Ser Glu Gly Val Met Asp Glu Gln Gly Lys Glu Phe Lys Glu Ile
195 200 205
Thr Ala Asn Gly Leu Lys Leu Gly Ala Ser Leu Ala Met Ala Glu His
210 215 220
Ile Pro Trp Leu Arg Trp Leu Phe Pro Leu Asp Glu Ala Ala Phe Ala
225 230 235 240
Lys His Gly Ala Arg Arg Asp Arg Leu Thr Arg Ala Ile Met Glu Glu
245 250 255
His Arg Leu Ala Arg Glu Lys Ser Gly Gly Ala Lys Gln His Phe Val
260 265 270
Asp Ala Leu Leu Thr Leu Lys Asp Lys Tyr Asp Leu Ser Glu Asp Thr
275 280 285
Ile Ile Gly Leu Leu Trp Asp Met Ile Thr Ala Gly Met Asp Thr Thr
290 295 300
Ala Ile Ser Val Glu Trp Ala Met Ala Glu Val Ile Lys Asn Pro Arg
305 310 315 320
Val Gln Gln Lys Val Gln Glu Glu Leu Asp Gln Val Ile Gly Tyr Glu
325 330 335
Arg Val Met Ile Glu Thr Asp Phe Ser Asn Leu Pro Tyr Leu Gln Ser
340 345 350
Val Ala Lys Glu Ser Leu Arg Leu His Pro Pro Thr Pro Leu Met Leu
355 360 365
Pro His Arg Ser Asn Ala Ser Val Lys Ile Gly Gly Tyr Asp Ile Pro
370 375 380
Lys Gly Ser Asn Val His Val Asn Val Trp Ala Val Ala Arg Asp Pro
385 390 395 400
Ala Val Trp Arg Asn Pro Leu Glu Phe Arg Pro Glu Arg Phe Leu Glu
405 410 415
Glu Asp Val Asp Met Lys Gly His Asp Phe Arg Leu Leu Pro Phe Gly
420 425 430
Ala Gly Arg Arg Val Cys Pro Gly Ala Gln Leu Gly Ile Asn Leu Val
435 440 445
Thr Ser Met Leu Gly His Leu Leu His His Phe Asn Trp Ala Pro Pro
450 455 460
His Gly Leu Ser Pro Asp Glu Ile Asp Met Gly Glu Ser Pro Gly Leu
465 470 475 480
Val Thr Tyr Met Arg Thr Ala Leu Arg Ala Val Pro Thr Pro Arg Leu
485 490 495
Pro Ser His Leu Tyr Glu Arg Val Ala Val Asp Met
500 505
<210> 179
<211> 512
<212> PRT
<213> 罗勒()
<220>
<223> 罗勒豆酰基莽草酸3-羟化酶 (ObC3H)
<400> 179
Met Ala Ala Leu Leu Leu Leu Leu Leu Leu Pro Leu Leu Leu Pro Ala
1 5 10 15
Ile Phe Leu Leu His His Leu Tyr Tyr Arg Leu Arg Phe Arg Leu Pro
20 25 30
Pro Gly Pro Arg Pro Leu Pro Ile Val Gly Asn Leu Tyr Asp Val Lys
35 40 45
Pro Val Arg Phe Arg Cys Phe Ala Asp Trp Ala Gln Ser Tyr Gly Pro
50 55 60
Ile Ile Ser Val Trp Phe Gly Ser Thr Leu Asn Val Ile Val Ser Asn
65 70 75 80
Thr Glu Leu Ala Lys Glu Val Leu Lys Glu Lys Asp Gln Gln Leu Ala
85 90 95
Asp Arg His Arg Ser Arg Ser Ala Ala Lys Phe Ser Arg Asp Gly Gln
100 105 110
Asp Leu Ile Trp Ala Asp Tyr Gly Pro His Tyr Val Lys Val Arg Lys
115 120 125
Val Cys Thr Leu Glu Leu Phe Ser Pro Lys Arg Leu Glu Ala Leu Arg
130 135 140
Pro Ile Arg Glu Asp Glu Val Thr Ala Met Val Glu Ser Ile Tyr His
145 150 155 160
Asp Cys Thr Ala Pro Asp Asn Ala Gly Lys Ser Leu Leu Val Lys Lys
165 170 175
Tyr Leu Gly Ala Val Ala Phe Asn Asn Ile Thr Arg Leu Ala Phe Gly
180 185 190
Lys Arg Phe Val Asn Ser Glu Gly Ile Ile Asp Lys Gln Gly Leu Glu
195 200 205
Phe Lys Ala Ile Val Ser Asn Gly Leu Lys Leu Gly Ala Ser Leu Ala
210 215 220
Met Ala Glu His Ile Pro Ser Leu Arg Trp Met Phe Pro Leu Asp Glu
225 230 235 240
Asp Ala Phe Ala Lys His Gly Ala Arg Arg Asp Gln Leu Thr Arg Glu
245 250 255
Ile Met Glu Glu His Thr Arg Ala Arg Glu Glu Ser Gly Gly Ala Lys
260 265 270
Gln His Phe Phe Asp Ala Leu Leu Thr Leu Lys Asp Lys Tyr Asp Leu
275 280 285
Ser Glu Asp Thr Ile Ile Gly Leu Leu Trp Asp Met Ile Thr Ala Gly
290 295 300
Met Asp Thr Thr Ala Ile Ser Val Glu Trp Ala Met Ala Glu Leu Ile
305 310 315 320
Lys Asn Pro Arg Val Gln Gln Lys Ala Gln Glu Glu Leu Asp Arg Val
325 330 335
Ile Gly Tyr Glu Arg Val Met Thr Glu Leu Asp Phe Ser Asn Leu Pro
340 345 350
Tyr Leu Gln Cys Val Ala Lys Glu Ala Leu Arg Leu His Pro Pro Thr
355 360 365
Pro Leu Met Leu Pro His Arg Ser Asn Ser Asn Val Lys Ile Gly Gly
370 375 380
Tyr Asp Ile Pro Lys Gly Ser Asn Val His Val Asn Val Trp Ala Val
385 390 395 400
Ala Arg Asp Pro Ala Val Trp Lys Asn Pro Cys Glu Phe Arg Pro Glu
405 410 415
Arg Phe Leu Glu Glu Asp Val Asp Met Lys Gly His Asp Phe Arg Leu
420 425 430
Leu Pro Phe Gly Ala Gly Arg Arg Val Cys Pro Gly Ala Gln Leu Gly
435 440 445
Ile Asn Leu Val Thr Ser Met Ile Gly His Leu Leu His His Phe Asn
450 455 460
Trp Ala Pro Pro Ser Gly Val Ser Ser Asp Glu Leu Asp Met Gly Glu
465 470 475 480
Asn Pro Gly Leu Val Thr Tyr Met Arg Thr Pro Leu Glu Ala Val Pro
485 490 495
Thr Pro Arg Leu Pro Ser Asp Leu Tyr Lys Arg Ile Ala Val Asp Leu
500 505 510
<210> 180
<211> 512
<212> PRT
<213> 火炬松()
<220>
<223> 松树豆酰基莽草酸3-羟化酶 (PtC3H)
<400> 180
Met Pro Val Pro Glu Met Gly Pro Ile Gly Gly Val Val Thr Gly Ile
1 5 10 15
Val Ala Ile Ala Ile Val Tyr Lys Leu Val Gln Trp Trp Arg Phe Lys
20 25 30
Leu Pro Pro Gly Pro Arg Pro Trp Pro Val Val Gly Asn Leu Leu Gln
35 40 45
Ile Lys Pro Val Arg Phe Arg Cys Phe Trp Asp Trp Ser Lys Lys Tyr
50 55 60
Gly Pro Ile Met Ser Val Trp Phe Gly Ser Thr Leu Asn Val Val Val
65 70 75 80
Ser Ser Thr Glu Leu Ala Lys Glu Val Leu Lys Glu His Asp Gln Gln
85 90 95
Leu Ala Asp Arg Pro Arg Ser Arg Ser Ala Glu Lys Phe Ser Arg His
100 105 110
Gly Gln Asp Leu Ile Trp Ala Asp Tyr Gly Pro His Tyr Val Lys Val
115 120 125
Arg Lys Val Cys Thr Leu Glu Leu Phe Ser Pro Lys Arg Leu Glu Ala
130 135 140
Leu Arg Pro Val Arg Glu Asp Glu Val Ala Ala Met Val Glu Ser Ile
145 150 155 160
Phe Asn Asp Cys Ser Lys Gln Glu Gly Ile Gly Lys Pro Leu Val Val
165 170 175
Lys Lys Tyr Leu Ser Ala Val Ala Phe Asn Asn Ile Thr Arg Ile Ala
180 185 190
Phe Gly Lys Arg Phe Val Asn Glu Glu Gly Lys Met Asp Pro Gln Gly
195 200 205
Leu Glu Phe Lys Glu Ile Val Ala Thr Gly Leu Lys Leu Gly Ala Ser
210 215 220
Leu Thr Met Ala Glu His Ile Pro Tyr Leu Arg Trp Met Phe Pro Leu
225 230 235 240
Glu Glu Gly Ala Phe Ala Lys His Gly Ala Arg Arg Asp Asn Val Thr
245 250 255
Lys Ala Ile Met Glu Glu His Thr Leu Ala Arg Gln Thr Ser Gly Ala
260 265 270
Lys Gln His Phe Val Asp Ala Leu Leu Thr Leu Gln Glu Lys Tyr Asp
275 280 285
Leu Ser Glu Asp Thr Ile Ile Gly Leu Leu Trp Asp Met Ile Thr Ala
290 295 300
Gly Met Asp Thr Thr Ala Ile Thr Val Glu Trp Ala Met Ala Glu Leu
305 310 315 320
Val Arg Asn Pro Arg Ile Gln Gln Lys Ala Gln Glu Glu Ile Asp Arg
325 330 335
Val Val Gly Arg Asp Arg Val Leu Asn Glu Thr Asp Phe Pro His Leu
340 345 350
Pro Tyr Leu Gln Cys Ile Thr Lys Glu Ala Leu Arg Leu His Pro Pro
355 360 365
Thr Pro Leu Met Leu Pro His Lys Ala Thr Gln Asn Val Lys Ile Gly
370 375 380
Gly Tyr Asp Ile Pro Lys Gly Ser Asn Val His Val Asn Val Trp Ala
385 390 395 400
Val Ala Arg Asp Pro Ala Val Trp Lys Asp Pro Val Thr Phe Arg Pro
405 410 415
Glu Arg Phe Ile Glu Glu Asp Val Asp Ile Lys Gly His Asp Tyr Arg
420 425 430
Leu Leu Pro Phe Gly Ala Gly Arg Arg Ile Cys Pro Gly Ala Gln Leu
435 440 445
Gly Ile Asn Leu Val Gln Ser Met Leu Gly His Leu Leu His His Phe
450 455 460
Glu Trp Ala Pro Pro Glu Gly Met Lys Ala Glu Asp Ile Asp Leu Thr
465 470 475 480
Glu Asn Pro Gly Leu Val Thr Phe Met Ala Lys Pro Val Gln Ala Ile
485 490 495
Ala Ile Pro Arg Leu Pro Ala His Leu Tyr Lys Arg Gln Pro Leu Asn
500 505 510
<210> 181
<211> 512
<212> PRT
<213> 火炬松()
<220>
<223> 松树豆酰基莽草酸3-羟化酶 (PtC3H)
<400> 181
Met Ser Val Pro Glu Met Gly Pro Phe Gly Val Val Val Thr Gly Ile
1 5 10 15
Val Ala Ile Ala Ile Val Tyr Lys Leu Val Gln Arg Trp Arg Phe Lys
20 25 30
Leu Pro Pro Gly Pro Arg Pro Trp Pro Val Val Gly Asn Leu Leu Gln
35 40 45
Ile Glu Pro Val Arg Phe Arg Cys Phe Trp Asp Trp Ser Lys Lys Tyr
50 55 60
Gly Pro Ile Met Ser Val Trp Phe Gly Ser Thr Leu Asn Val Val Val
65 70 75 80
Ser Asn Thr Glu Leu Ala Lys Glu Val Leu Lys Glu His Asp Gln Gln
85 90 95
Leu Ala Asp Arg Pro Arg Ser Arg Ser Ala Glu Lys Phe Ser Arg Asn
100 105 110
Gly Gln Asp Leu Ile Trp Ala Asp Tyr Gly Pro His Tyr Val Lys Val
115 120 125
Arg Lys Val Cys Thr Leu Glu Leu Phe Ser Pro Lys Arg Leu Glu Ala
130 135 140
Leu Arg Pro Ile Arg Glu Asp Glu Val Ala Ala Met Val Glu Ser Ile
145 150 155 160
Phe Asn Asp Cys Gly Lys Gln Glu Gly Ile Gly Lys Pro Leu Val Val
165 170 175
Lys Arg Tyr Leu Ser Gly Val Ala Phe Asn Asn Ile Thr Arg Pro Ala
180 185 190
Phe Gly Lys Arg Phe Val Asn Glu Glu Gly Lys Met Asp Pro Gln Gly
195 200 205
Val Glu Phe Lys Glu Ile Val Ala Thr Gly Leu Lys Leu Gly Ala Ser
210 215 220
Leu Thr Met Ala Glu His Ile Pro Tyr Leu Arg Trp Met Phe Pro Leu
225 230 235 240
Glu Glu Gly Ala Phe Ala Lys His Gly Ala Arg Arg Asp Asn Val Thr
245 250 255
Lys Ala Ile Met Glu Glu His Thr Leu Ala Arg Gln Thr Ser Gly Ala
260 265 270
Lys Gln His Phe Val Asp Ala Leu Leu Thr Leu Gln Glu Lys Tyr Asp
275 280 285
Leu Ser Glu Asp Thr Ile Ile Gly Leu Leu Trp Asp Met Ile Thr Ala
290 295 300
Gly Met Asp Thr Thr Ala Ile Thr Val Glu Trp Ala Met Ala Glu Leu
305 310 315 320
Val Arg Asn Pro Arg Ile Gln Gln Lys Ala Gln Glu Glu Ile Asp Arg
325 330 335
Val Val Gly Arg Asp Arg Val Met Asn Glu Thr Asp Phe Pro His Leu
340 345 350
Pro Tyr Leu Gln Cys Ile Thr Lys Glu Ala Leu Arg Leu His Pro Pro
355 360 365
Thr Pro Leu Met Leu Pro His Lys Ala Thr Gln Asn Val Lys Ile Gly
370 375 380
Gly Tyr Asp Ile Pro Lys Gly Ser Asn Val His Val Asn Val Trp Ala
385 390 395 400
Ile Ala Arg Asp Pro Ala Val Trp Lys Asp Pro Val Thr Phe Arg Pro
405 410 415
Glu Arg Phe Leu Glu Glu Asp Val Asp Ile Lys Gly His Asp Tyr Arg
420 425 430
Leu Leu Pro Phe Gly Ala Gly Arg Arg Ile Cys Pro Gly Ala Gln Leu
435 440 445
Gly Ile Asn Leu Val Gln Ser Met Leu Gly His Leu Leu His His Phe
450 455 460
Val Trp Ala Pro Pro Glu Gly Met Gln Ala Glu Asp Ile Asp Leu Thr
465 470 475 480
Glu Asn Pro Gly Leu Val Thr Phe Met Ala Lys Pro Val Gln Ala Ile
485 490 495
Ala Ile Pro Arg Leu Pro Asp His Leu Tyr Lys Arg Gln Pro Leu Asn
500 505 510
<210> 182
<211> 508
<212> PRT
<213> 红花烟草()
<220>
<223> 烟草豆酰基莽草酸3-羟化酶 (NtC3H)
<400> 182
Met Ala Leu Ser Phe Ile Phe Ile Ser Ile Thr Leu Ile Phe Leu Val
1 5 10 15
His Lys Leu Tyr His Arg Leu Arg Phe Lys Leu Pro Pro Gly Pro Arg
20 25 30
Pro Leu Pro Val Val Gly Asn Leu Tyr Asp Ile Lys Pro Val Arg Phe
35 40 45
Arg Cys Phe Ala Asp Trp Ala Lys Thr Tyr Gly Pro Ile Phe Ser Val
50 55 60
Tyr Phe Gly Ser Gln Leu Asn Val Val Val Thr Thr Ala Glu Leu Ala
65 70 75 80
Lys Glu Val Leu Lys Glu Asn Asp Gln Asn Leu Ala Asp Arg Phe Arg
85 90 95
Thr Arg Pro Ala Asn Asn Leu Ser Arg Asn Gly Met Asp Leu Ile Trp
100 105 110
Ala Asp Tyr Gly Pro His Tyr Val Lys Val Arg Lys Leu Cys Asn Leu
115 120 125
Glu Leu Phe Thr Pro Lys Arg Leu Glu Ala Leu Arg Pro Ile Arg Glu
130 135 140
Asp Glu Val Thr Ala Met Val Glu Asn Ile Phe Lys Asp Cys Thr Lys
145 150 155 160
Pro Asp Asn Thr Gly Lys Ser Leu Leu Ile Arg Glu Tyr Leu Gly Ser
165 170 175
Val Ala Phe Asn Asn Ile Thr Arg Leu Thr Phe Gly Lys Arg Phe Met
180 185 190
Asn Ser Lys Gly Glu Ile Asp Glu Gln Gly Gln Glu Phe Lys Gly Ile
195 200 205
Val Ser Asn Gly Ile Lys Ile Gly Gly Lys Leu Pro Leu Ala Glu Tyr
210 215 220
Val Pro Trp Leu Arg Trp Phe Phe Thr Met Glu Asn Glu Ala Leu Val
225 230 235 240
Lys His Ser Ala Arg Arg Asp Arg Leu Thr Arg Met Ile Met Asp Glu
245 250 255
His Thr Leu Ala Arg Lys Lys Thr Gly Asp Thr Lys Gln His Phe Val
260 265 270
Asp Ala Leu Leu Thr Leu Gln Lys Gln Tyr Asp Leu Ser Asp Asp Thr
275 280 285
Val Ile Gly Leu Leu Trp Asp Met Ile Thr Ala Gly Met Asp Thr Thr
290 295 300
Thr Ile Thr Val Glu Trp Ala Met Ala Glu Leu Val Lys Asn Pro Arg
305 310 315 320
Val Gln Leu Lys Ala Gln Glu Glu Leu Asp Arg Val Ile Gly Thr Asp
325 330 335
Arg Ile Met Ser Glu Thr Asp Phe Ser Lys Leu Pro Tyr Leu Gln Cys
340 345 350
Val Ala Lys Glu Ala Leu Arg Leu His Pro Pro Thr Pro Leu Met Leu
355 360 365
Pro His Lys Ala Ser Ala Ser Val Lys Ile Gly Gly Tyr Asp Ile Pro
370 375 380
Lys Gly Ser Ile Val His Val Asn Val Trp Ala Val Ala Arg Asp Pro
385 390 395 400
Ala Val Trp Lys Asn Pro Leu Glu Phe Arg Pro Glu Arg Phe Leu Glu
405 410 415
Glu Asp Val Asp Met Lys Gly His Asp Tyr Arg Leu Leu Pro Phe Gly
420 425 430
Ala Gly Arg Arg Val Cys Pro Gly Ala Gln Leu Ala Ile Asn Leu Val
435 440 445
Thr Ser Met Leu Gly His Leu Leu His His Phe Thr Trp Ala Pro Ala
450 455 460
Pro Gly Val Asn Pro Glu Asp Ile Asp Leu Glu Glu Ser Pro Gly Thr
465 470 475 480
Val Thr Tyr Met Lys Asn Pro Ile Gln Ala Ile Pro Thr Pro Arg Leu
485 490 495
Pro Ala His Leu Tyr Gly Arg Val Pro Val Asp Met
500 505
<210> 183
<211> 508
<212> PRT
<213> 银杏树()
<220>
<223> 豆酰基莽草酸3-羟化酶 (GbC3H)
<400> 183
Met Ser Pro Leu Ser Ser Val Leu Ile Gly Phe Val Val Ile Leu Val
1 5 10 15
Val Tyr Lys Val Val Gln Trp Lys Arg Tyr Lys Leu Pro Pro Gly Pro
20 25 30
Arg Pro Leu Pro Val Val Gly Asn Leu Leu Asp Ile Ala Pro Val Arg
35 40 45
Phe Arg Cys Phe Ser Glu Trp Ser Gln Lys Tyr Gly Pro Ile Met Ser
50 55 60
Val Trp Phe Gly Ser Thr Leu Asn Val Ile Val Cys Asn Thr Glu Leu
65 70 75 80
Ala Lys Glu Val Leu Lys Glu Asn Asp Gln Leu Leu Ala Asp Arg Pro
85 90 95
Arg Ser Arg Ser Ser Glu Lys Phe Ser Arg Asn Gly Gln Asp Leu Ile
100 105 110
Trp Ala Asp Tyr Gly Pro His Tyr Val Lys Val Arg Lys Val Cys Thr
115 120 125
Leu Glu Leu Phe Thr Pro Lys Arg Leu Glu Ser Leu Arg Pro Val Arg
130 135 140
Glu Asp Glu Val Ala Ala Met Val Glu Ser Ile Phe Lys Asp Cys Gly
145 150 155 160
Ser Gln Glu Gly Val Gly Lys Thr Val Met Val Lys Lys Tyr Pro Ser
165 170 175
Ala Val Ala Phe Asn Asn Ile Thr Arg Ile Ala Phe Gly Lys Arg Phe
180 185 190
Val Asn Gly Glu Gly Lys Met Asp Pro Gln Gly Ile Glu Phe Lys Glu
195 200 205
Ile Val Gly Thr Gly Leu Lys Leu Gly Ala Ser Leu Thr Met Ala Glu
210 215 220
His Ile Pro Tyr Leu Arg Trp Met Phe Pro Leu Glu Glu Gly Ala Phe
225 230 235 240
Ala Lys His Gly Ala Arg Arg Asp Asn Leu Thr Lys Ala Ile Met Glu
245 250 255
Glu His Thr Leu Ala Arg Gln Lys Ser Gly Ala Lys Gln His Phe Val
260 265 270
Asp Ala Leu Leu Thr Leu Gln Glu Lys Tyr Asp Leu Ser Met Asp Thr
275 280 285
Ile Ile Gly Leu Leu Trp Asp Met Ile Thr Ala Gly Met Asp Thr Pro
290 295 300
Ala Ile Thr Val Glu Trp Pro Met Ala Glu Leu Val Arg Asn Pro Arg
305 310 315 320
Val His Gln Lys Ala His Glu Glu Leu Asp Arg Val Ile Gly Lys Asp
325 330 335
Arg Val Ile Asn Glu Thr Asp Phe Ala His Leu Pro Tyr Leu Gln Cys
340 345 350
Ile Thr Lys Glu Ala Leu Arg Leu His Pro Pro Thr Pro Leu Met Leu
355 360 365
Pro His Lys Ala Ile Glu Asn Val Lys Ile Gly Gly Tyr Asp Ile Pro
370 375 380
Lys Gly Ser Asn Val His Val Asn Val Trp Ala Ile Ala Arg Asp Pro
385 390 395 400
Ala Val Trp Lys Asp Pro Val Ala Phe Arg Pro Glu Arg Phe Ile Glu
405 410 415
Glu Asp Val Asp Ile Lys Gly His Asp Tyr Arg Leu Leu Pro Phe Gly
420 425 430
Ala Gly Arg Arg Val Cys Pro Gly Ala Gln Leu Gly Leu Asn Leu Val
435 440 445
Gln Ser Met Leu Gly His Leu Leu His His Phe Ile Trp Ala Pro Pro
450 455 460
Glu Gly Met Lys Ser Glu Gly Ile Asp Leu Thr Glu Ser Pro Gly Leu
465 470 475 480
Val Thr Phe Met Ala Lys Pro Val Glu Ala Phe Ala Ile Pro Arg Leu
485 490 495
Pro Ala Pro Leu Tyr Lys Arg Glu Pro Val Asn Trp
500 505
<210> 184
<211> 512
<212> PRT
<213> 两色高梁()
<220>
<223> 高粱豆酰基莽草酸3-羟化酶 (SbC3H)
<400> 184
Met Asp Ala Ser Leu Leu Leu Ser Val Ala Leu Ala Val Val Leu Ile
1 5 10 15
Pro Leu Ser Leu Ala Leu Leu Asn Arg Leu Arg Leu Gly Arg Leu Pro
20 25 30
Pro Gly Pro Arg Pro Trp Pro Val Leu Gly Asn Leu Arg Gln Ile Lys
35 40 45
Pro Ile Arg Cys Arg Cys Phe Gln Glu Trp Ala Glu Arg Tyr Gly Pro
50 55 60
Val Ile Ser Val Trp Phe Gly Ser Gly Leu Thr Val Val Val Ser Thr
65 70 75 80
Ser Glu Leu Ala Lys Glu Val Leu Lys Glu Asn Asp Gln Gln Leu Ala
85 90 95
Asp Arg Pro Arg Asn Arg Ser Thr Gln Arg Phe Ser Arg Asn Gly Gln
100 105 110
Asp Leu Ile Trp Ala Asp Tyr Gly Pro His Tyr Ile Lys Val Arg Lys
115 120 125
Leu Cys Asn Leu Glu Leu Phe Thr Pro Lys Arg Leu Glu Ala Leu Arg
130 135 140
Pro Ile Arg Glu Asp Glu Val Thr Ala Met Val Glu Ser Val Tyr Arg
145 150 155 160
Ala Ala Thr Ala Pro Gly Asn Glu Gly Lys Pro Met Val Val Arg Asn
165 170 175
His Leu Ser Met Val Ala Phe Asn Asn Ile Thr Arg Leu Ala Phe Gly
180 185 190
Lys Arg Phe Met Asn Ala Asn Gly Asp Ile Asp Glu Gln Gly Arg Glu
195 200 205
Phe Lys Thr Ile Val Asn Asn Gly Ile Lys Ile Gly Ala Ser Leu Ser
210 215 220
Val Ala Glu Phe Ile Trp Tyr Leu Arg Trp Leu Cys Pro Leu Asn Glu
225 230 235 240
Glu Leu Tyr Lys Thr His Asn Glu Arg Arg Asp Arg Leu Thr Met Lys
245 250 255
Ile Ile Glu Glu His Ala Lys Ser Leu Lys Glu Ser Gly Ala Lys Gln
260 265 270
His Phe Val Asp Ala Leu Phe Thr Leu Lys Gln Gln Tyr Asp Leu Ser
275 280 285
Glu Asp Thr Val Ile Gly Leu Leu Trp Asp Met Ile Thr Ala Gly Met
290 295 300
Asp Thr Thr Val Ile Ser Val Glu Trp Ala Met Ala Glu Leu Val Arg
305 310 315 320
Asn Pro Arg Val Gln Lys Lys Leu Gln Glu Glu Leu Asp Arg Val Val
325 330 335
Gly Arg Asp Arg Val Met Leu Glu Thr Asp Phe Gln Asn Leu Pro Tyr
340 345 350
Leu Gln Ala Val Val Lys Glu Ser Leu Arg Leu His Pro Pro Thr Pro
355 360 365
Leu Met Leu Pro His Lys Ala Ser Thr Asn Val Lys Ile Gly Gly Tyr
370 375 380
Asp Ile Pro Lys Gly Ala Asn Val Met Val Asn Val Trp Ala Val Ala
385 390 395 400
Arg Asp Pro Lys Val Trp Ser Asn Pro Leu Glu Tyr Arg Pro Glu Arg
405 410 415
Phe Leu Glu Glu Asn Ile Asp Ile Lys Gly Ser Asp Phe Arg Val Leu
420 425 430
Pro Phe Gly Ala Gly Arg Arg Val Cys Pro Gly Ala Gln Leu Gly Ile
435 440 445
Asn Leu Val Ala Ser Met Ile Gly His Leu Leu His His Phe Glu Trp
450 455 460
Ser Leu Pro Glu Gly Thr Arg Pro Glu Asp Val Asn Met Met Glu Ser
465 470 475 480
Pro Gly Leu Val Thr Phe Met Gly Thr Pro Leu Gln Ala Val Ala Lys
485 490 495
Pro Arg Leu Glu Lys Glu Glu Leu Tyr Asn Arg Val Pro Val Glu Met
500 505 510
<210> 185
<211> 512
<212> PRT
<213> 玉米()
<220>
<223> 玉米豆酰基莽草酸3-羟化酶 (ZmC3H)
<400> 185
Met Asp Ala Ser Leu Leu Leu Ser Val Gly Leu Ala Leu Val Leu Ile
1 5 10 15
Pro Leu Ser Leu Ser Leu Leu Asn Arg Leu Arg Val Gly Arg Leu Pro
20 25 30
Pro Gly Pro Arg Pro Trp Pro Val Leu Gly Asn Leu Arg Gln Ile Lys
35 40 45
Pro Val Arg Cys Arg Cys Phe Gln Glu Trp Ala Glu Arg Tyr Gly Pro
50 55 60
Ile Ile Ser Val Trp Phe Gly Ser Gly Leu Thr Val Val Val Ser Thr
65 70 75 80
Ser Glu Leu Ala Lys Glu Val Leu Lys Glu Asn Asp Gln Gln Leu Ala
85 90 95
Asp Arg Pro Arg Asn Arg Ser Thr Gln Arg Phe Ser Arg Asn Gly Gln
100 105 110
Asp Leu Ile Trp Ala Asp Tyr Gly Pro His Tyr Ile Lys Val Arg Lys
115 120 125
Leu Cys Asn Leu Glu Leu Phe Thr Pro Arg Arg Leu Glu Ala Leu Arg
130 135 140
Pro Ile Arg Glu Asp Glu Val Thr Ala Met Val Glu Ser Val His Arg
145 150 155 160
Ala Ala Thr Ala Pro Gly Asn Glu Gly Lys Pro Met Val Val Arg Lys
165 170 175
His Leu Ser Met Val Ala Phe Asn Asn Ile Thr Arg Leu Ala Phe Gly
180 185 190
Lys Arg Phe Met Asn Ala Asn Gly Asp Val Asp Glu Gln Gly Arg Glu
195 200 205
Phe Lys Thr Ile Val Asn Asn Gly Ile Lys Ile Gly Ala Ser Leu Ser
210 215 220
Val Ala Glu Phe Ile Trp Tyr Leu Arg Trp Leu Cys Pro Leu Asn Glu
225 230 235 240
Glu Leu Tyr Lys Thr His Asn Glu Arg Arg Asp Arg Leu Thr Met Lys
245 250 255
Ile Ile Asp Glu His Ala Lys Ser Leu Lys Glu Ser Gly Ala Lys Gln
260 265 270
His Phe Val Asp Ala Leu Phe Thr Leu Lys Gln Gln Tyr Asp Leu Ser
275 280 285
Glu Asp Thr Val Ile Gly Leu Leu Trp Asp Met Ile Thr Ala Gly Met
290 295 300
Asp Thr Thr Val Ile Ser Val Glu Trp Ala Met Ala Glu Leu Val Arg
305 310 315 320
Asn Pro Arg Val Gln Lys Lys Leu Gln Glu Glu Leu Asp Arg Val Val
325 330 335
Gly Arg Asp Arg Val Met Leu Glu Thr Asp Phe Gln Asn Leu Pro Tyr
340 345 350
Leu Gln Ala Val Val Lys Glu Ser Leu Arg Leu His Pro Pro Thr Pro
355 360 365
Leu Met Leu Pro His Lys Ala Ser Ser Asn Val Lys Ile Gly Gly Tyr
370 375 380
Asn Ile Pro Lys Gly Ala Asn Val Met Val Asn Val Trp Ala Val Ala
385 390 395 400
Arg Asp Pro Lys Val Trp Ser Asn Pro Leu Glu Tyr Arg Pro Glu Arg
405 410 415
Phe Leu Glu Glu Asn Ile Asp Ile Lys Gly Ser Asp Phe Arg Val Leu
420 425 430
Pro Phe Gly Ala Gly Arg Arg Val Cys Pro Gly Ala Gln Leu Gly Ile
435 440 445
Asn Leu Val Ala Ser Met Ile Gly His Leu Leu His His Phe Glu Trp
450 455 460
Ser Leu Pro Glu Gly Thr Arg Pro Glu Asp Val Asn Met Met Glu Ser
465 470 475 480
Pro Gly Leu Val Thr Phe Met Gly Thr Pro Leu Gln Ala Val Val Lys
485 490 495
Pro Arg Leu Glu Lys Glu Glu Leu Tyr Asn Arg Val Pro Val Glu Met
500 505 510
<210> 186
<211> 512
<212> PRT
<213> 水稻()
<220>
<223> 稻豆酰基莽草酸3-羟化酶 (OsC3H)
<400> 186
Met Asp Val Ala Ser Leu Leu Pro Phe Ala Leu Ala Leu Val Ala Ile
1 5 10 15
Pro Ile Ser Leu Ala Leu Leu Asp Arg Leu Arg Leu Gly Arg Ile Pro
20 25 30
Pro Gly Pro Arg Pro Trp Pro Met Val Gly Asn Leu Trp Gln Ile Lys
35 40 45
Pro Val Arg Cys Arg Gly Phe Leu Glu Trp Ala Glu Arg Tyr Gly Pro
50 55 60
Ile Val Ser Val Trp Phe Gly Ser Ser Leu Asn Val Val Val Ser Thr
65 70 75 80
Ser Glu Leu Ala Lys Glu Val Leu Lys Glu Asn Asp Gln Leu Leu Ala
85 90 95
Asp Arg Pro Arg Asn Arg Ser Thr Gln Arg Phe Ser Arg Asn Gly Met
100 105 110
Asp Leu Ile Trp Ala Asp Tyr Gly Pro His Tyr Ile Lys Val Arg Lys
115 120 125
Leu Cys Asn Leu Glu Leu Phe Thr Pro Lys Arg Leu Glu Ala Leu Arg
130 135 140
Pro Ile Arg Glu Asp Glu Val Thr Ala Met Val Glu Ser Val His Arg
145 150 155 160
Ala Val Thr Gln Pro Gly Ser Glu His Lys Pro Ile Val Val Arg Asn
165 170 175
His Leu Ala Met Val Ala Phe Asn Asn Ile Thr Arg Leu Ala Phe Gly
180 185 190
Lys Arg Phe Met Asn Ala Asn Gly Asp Ile Asp Glu Gln Gly Arg Glu
195 200 205
Phe Lys Thr Ile Val Asn Asn Gly Ile Lys Ile Gly Ala Ser Leu Ser
210 215 220
Val Ala Glu Tyr Ile Trp Tyr Leu Arg Trp Leu Cys Pro Leu Asn Glu
225 230 235 240
Glu Leu Tyr Lys Thr His Asn Glu Arg Arg Asp Arg Leu Thr Lys Lys
245 250 255
Ile Ile Asp Glu His Ala Lys Ala Leu Lys Glu Ser Gly Ala Lys Gln
260 265 270
His Phe Val Asp Ala Leu Phe Thr Leu Arg Glu Gln Tyr Asp Leu Ser
275 280 285
Asp Asp Thr Val Ile Gly Leu Leu Trp Asp Met Ile Thr Ala Gly Met
290 295 300
Asp Thr Thr Val Ile Ser Val Glu Trp Ala Met Ala Glu Leu Val Arg
305 310 315 320
Asn Pro Arg Val Gln Lys Lys Leu Gln Glu Glu Leu Asp Arg Val Val
325 330 335
Gly Arg Asp Arg Val Met Ser Glu Thr Asp Phe Gln Ser Leu Pro Tyr
340 345 350
Leu Asn Ala Val Val Lys Glu Ser Leu Arg Leu His Pro Pro Thr Pro
355 360 365
Leu Met Leu Pro His Lys Ala Ser Thr Asn Val Lys Ile Gly Gly Tyr
370 375 380
Asn Ile Pro Lys Gly Ala Asn Val Met Val Asn Val Trp Ala Ile Ala
385 390 395 400
Arg Asp Pro Lys Val Trp Ser Asn Pro Leu Glu Tyr Arg Pro Glu Arg
405 410 415
Phe Ile Glu Glu Asn Ile Asp Ile Lys Gly Ser Asp Phe Arg Val Leu
420 425 430
Pro Phe Gly Ala Gly Arg Arg Val Cys Pro Gly Ala Gln Leu Gly Ile
435 440 445
Asn Leu Val Ala Ser Met Ile Gly His Leu Leu His Gln Phe Glu Trp
450 455 460
Ser Leu Pro Glu Gly Thr Arg Pro Glu Asp Val Asn Met Met Glu Ser
465 470 475 480
Asn Gly Val Val Thr Phe Met Ser Thr Ser Leu Gln Val Ile Ala Lys
485 490 495
Pro Arg Leu Asp Asn Pro Asp Leu Tyr Lys Arg Phe Pro Val Glu Met
500 505 510
<210> 187
<211> 512
<212> PRT
<213> 小麦()
<220>
<223> 小麦豆酰基莽草酸3-羟化酶 (TaC3H)
<400> 187
Met Asp Val Ala Ser Leu Leu Pro Phe Ala Ile Ala Leu Val Ala Ile
1 5 10 15
Pro Ile Ser Leu Ala Leu Leu Asp Arg Leu Arg Leu Gly Arg Leu Pro
20 25 30
Pro Gly Pro Arg Pro Trp Pro Val Val Gly Asn Leu Arg Gln Ile Lys
35 40 45
Pro Val Arg Cys Arg Cys Phe Gln Glu Trp Ala Ala Arg Tyr Gly Pro
50 55 60
Ile Ile Ser Val Trp Phe Gly Ser Ser Leu Thr Val Val Val Ser Thr
65 70 75 80
Ser Glu Leu Ala Lys Glu Val Leu Lys Glu His Asp Gln Gln Leu Ala
85 90 95
Asp Arg Pro Arg Asn Arg Ser Thr Gln Arg Phe Ser Arg Asn Gly Gln
100 105 110
Asp Leu Ile Trp Ala Asp Tyr Gly Pro His Tyr Ile Lys Val Arg Lys
115 120 125
Leu Cys Asn Leu Glu Leu Phe Thr Gln Lys Arg Leu Glu Ala Leu Arg
130 135 140
Pro Ile Arg Glu Asp Glu Val Thr Ala Met Val Glu Ser Val His Arg
145 150 155 160
Ala Ala Ala Gly Pro Gly Asn Glu Gly Lys Pro Leu Val Val Arg Asn
165 170 175
His Leu Ala Met Val Ser Phe Asn Asn Ile Thr Arg Leu Ala Phe Gly
180 185 190
Lys Arg Phe Met Asn Ala Asp Gly Asp Ile Asp Glu Glu Gly Gln Glu
195 200 205
Phe Lys Leu Ile Val Asn Asn Gly Ile Lys Ile Gly Ala Ser Leu Ser
210 215 220
Val Ala Glu Tyr Ile Trp Tyr Leu Arg Trp Leu Cys Pro Leu Asn Glu
225 230 235 240
Glu Leu Tyr Asn Thr His Asn Glu Arg Arg Asp Arg Leu Thr Lys Lys
245 250 255
Ile Ile Glu Glu His Ala Gln Ala Leu Arg Glu Ser Gly Ala Lys Gln
260 265 270
His Phe Val Asp Ala Leu Phe Thr Leu Arg Glu Lys Tyr Asp Leu Ser
275 280 285
Asp Asp Thr Val Phe Gly Leu Leu Trp Asp Met Ile Thr Ala Gly Met
290 295 300
Asp Thr Thr Val Ile Ser Val Glu Trp Ala Met Ala Glu Leu Val Arg
305 310 315 320
Asn Pro Arg Val Gln Lys Lys Leu Gln Glu Glu Leu Asp Ser Val Val
325 330 335
Gly Arg Asp Arg Val Met Ser Glu Thr Asp Phe Gln Asn Leu Pro Tyr
340 345 350
Leu Met Ala Val Val Lys Glu Ser Leu Arg Leu His Pro Pro Thr Pro
355 360 365
Leu Met Leu Pro His Lys Ala Ser Ala Ser Val Lys Val Gly Gly Tyr
370 375 380
Ser Ile Pro Lys Gly Ala Asn Val Met Val Asn Val Trp Ala Val Ala
385 390 395 400
Arg Asp Pro Lys Val Trp Ser Ser Pro Leu Glu Phe Arg Pro Glu Arg
405 410 415
Phe Leu Glu Glu Ser Ile Asp Ile Lys Gly Ser Asp Phe Arg Val Leu
420 425 430
Pro Phe Gly Ala Gly Arg Arg Val Cys Pro Gly Ala Gln Leu Gly Ile
435 440 445
Asn Leu Val Ala Ser Met Ile Gly His Met Leu His His Phe Glu Trp
450 455 460
Ser Leu Pro Glu Gly Ala Arg Pro Glu Asp Ile Ser Met Met Glu Ser
465 470 475 480
Pro Gly Leu Val Thr Phe Met Gly Thr Leu Leu Gln Ala Val Ala Thr
485 490 495
Pro Arg Leu Glu Asn Glu Glu Leu Tyr Lys Arg Val Pro Val Glu Ile
500 505 510
<210> 188
<211> 513
<212> PRT
<213> 水稻()
<220>
<223> 稻豆酰基莽草酸3-羟化酶 (OsC3H)
<400> 188
Met Asp Thr Pro Ala Leu Leu Pro Leu Ala Leu Ala Leu Val Ala Ile
1 5 10 15
Pro Ile Val Ile Leu Leu Phe Asn Arg Ile Arg Pro Gly Arg Leu Pro
20 25 30
Pro Gly Pro Arg Ala Trp Pro Val Val Gly Asn Leu Phe Asp Ile His
35 40 45
Pro Val Arg Cys Arg Cys Phe Met Glu Trp Ala Gly Lys Tyr Gly Pro
50 55 60
Ile Met Thr Val Trp Leu Gly Thr Ser Pro Thr Val Val Val Ser Thr
65 70 75 80
Ser Glu Leu Ala Arg Glu Val Leu Lys Asn Asn Asp Gln Gln Leu Ala
85 90 95
Asp Arg Pro Arg Asn Arg Ser Ser Glu Arg Phe Ser Arg Gly Gly Val
100 105 110
Asp Leu Ile Trp Ala Asp Tyr Gly Pro His Tyr Ile Lys Val Arg Lys
115 120 125
Leu Cys Asn Leu Glu Leu Phe Ala Pro Arg Arg Met Glu Ala Leu Arg
130 135 140
Pro Ile Arg Glu Asp Glu Val Thr Ala Met Val Glu Ser Ile Tyr Arg
145 150 155 160
Ala Ile Thr Ala Pro Gly Glu Glu Gly Lys Pro Met Val Met Arg Lys
165 170 175
His Leu Ser Met Val Ala Phe Asn Asn Ile Thr Arg Leu Thr Phe Gly
180 185 190
Lys Arg Phe Ile Asp Ala Ala Gly Glu Leu Asp Glu Gln Gly Ser Glu
195 200 205
Leu Lys Ala Ile Val Asn Asn Gly Ile Lys Ile Gly Ala Ser Leu Thr
210 215 220
Ile Ala Glu His Ile Arg Val Leu Arg Trp Leu Asn Pro Val Asp Glu
225 230 235 240
Glu Leu Tyr Asn Ala His Ser Ala Arg Arg Asp Arg Phe Thr Arg Arg
245 250 255
Ile Met Asp Glu His Ala Arg Glu Leu Glu Arg His Gly Ala Lys Gln
260 265 270
His Phe Val Asp Ala Leu Phe Thr Leu Arg Asp Lys Tyr Asp Leu Ser
275 280 285
Asp Asp Thr Val Ile Gly Leu Leu Trp Asp Met Ile Thr Ala Gly Thr
290 295 300
Asp Thr Thr Val Ile Thr Val Glu Trp Ala Met Ala Glu Leu Ala Arg
305 310 315 320
Asn Pro Arg Val Gln Met Lys Ala Gln Glu Glu Leu Asp Arg Val Ile
325 330 335
Gly Arg Gly Arg Val Met Leu Glu Ala Asp Ile Pro Asn Leu Pro Tyr
340 345 350
Leu Gln Ala Val Val Lys Glu Ser Phe Arg Leu His Pro Pro Thr Pro
355 360 365
Leu Met Leu Pro His Lys Ala Ser Thr Ser Val Lys Ile Ala Gly Tyr
370 375 380
Asp Val Pro Lys Asp Ala Ser Val Val Val Asn Val Trp Ala Val Ala
385 390 395 400
Arg Asp Pro Gly Val Trp Asp Asn Pro Leu Glu Tyr Arg Pro Glu Arg
405 410 415
Phe Leu Glu Glu Ser Ile Asp Ile Lys Gly Ser Asp Tyr Arg Val Leu
420 425 430
Pro Phe Gly Ala Gly Arg Arg Val Cys Pro Gly Ala Gln Leu Gly Ile
435 440 445
Ser Leu Val Ala Ser Met Ile Gly His Leu Leu His Gln Phe Thr Trp
450 455 460
Ala Leu Pro Asp Gly Thr Arg Pro Glu Asp Leu Asp Met Met Glu Ser
465 470 475 480
Pro Gly Leu Val Thr Phe Met Ala Thr Pro Leu Gln Val Val Ala Met
485 490 495
Pro Arg Leu Asp Lys Glu Glu Leu Phe Lys Arg Val Pro Val Asp Met
500 505 510
Ser
<210> 189
<211> 524
<212> PRT
<213> 江南卷柏()
<220>
<223> 卷柏豆酰基莽草酸3-羟化酶 (SmC3H)
<400> 189
Met Asp Pro Ser Ala Glu Ala Tyr His His His His His His Tyr Thr
1 5 10 15
Glu Phe Val Pro Pro Asp His Gly His Arg Tyr Ser Thr Trp Thr Ile
20 25 30
Pro Val Gly Leu Phe Leu Val Tyr Leu Thr Phe Lys Leu Leu Ser Arg
35 40 45
Pro Arg Leu Pro Pro Gly Pro Lys Pro Trp Pro Ile Ile Gly Ser Leu
50 55 60
His Arg Val Ser Pro Val Arg Phe Arg Cys Phe Met Asp Trp Ala Glu
65 70 75 80
Lys His Gly Pro Ile Met Ser Val Trp Met Gly Thr Asn Leu Asn Val
85 90 95
Val Ile Ser Asn Ala Glu Leu Ala Lys Glu Val Leu Lys Glu Lys Asp
100 105 110
Lys Glu Leu Ala Ser Arg Pro Leu Thr Arg Ala Ala Ala Arg Phe Ser
115 120 125
Arg Asn Gly Gln Asp Leu Ile Trp Ala Asp Tyr Gly Asp His Tyr Val
130 135 140
Lys Val Arg Lys Leu Cys Thr Leu Glu Leu Phe Thr Pro Lys Arg Leu
145 150 155 160
Glu Ser Leu Arg Trp Ile Arg Glu Glu Glu Val Gly Ala Met Val Lys
165 170 175
Ser Ile Phe Gln Asp Val Lys Asn Asn Gly Gly Ala Ala Val Thr Val
180 185 190
Lys Asn Tyr Val Ser Ala Val Ala Phe Asn Asn Ile Thr Arg Ile Val
195 200 205
Phe Gly Lys Arg Phe Val Asp Glu Asn Gly Glu Met Leu Pro Gln Gly
210 215 220
Val Glu Phe Lys Ala Ile Ile Ser Gln Gly Met Lys Leu Gly Ala Ser
225 230 235 240
Leu Thr Met Ala Glu His Ile Ser Phe Ile Arg Trp Met Phe Pro Leu
245 250 255
Gln Glu Glu Glu Phe Ala Lys His Gly Ala Arg Arg Asp Ser Leu Thr
260 265 270
Lys Glu Ile Met Glu Glu His Ala Leu Glu Lys Lys Lys Ser Gly Thr
275 280 285
Ser Gln Glu His Phe Val Asp Ala Leu Leu Asn Leu Lys Asp Gln Tyr
290 295 300
Gly Leu Ser Glu Thr Thr Val Ile Gly Leu Leu Trp Asp Met Ile Thr
305 310 315 320
Ala Gly Met Asp Thr Thr Ala Ile Ser Ala Glu Trp Ala Met Ala Glu
325 330 335
Ile Ile Lys His Pro Lys Val Gln Glu Lys Ala His Glu Glu Met Asp
340 345 350
Arg Val Ile Gly Lys Glu Arg Ile Ile Thr Glu Val Asp Val Gln Gln
355 360 365
Leu Pro Tyr Leu Gln Ser Ile Val Lys Glu Ala Leu Arg Leu His Pro
370 375 380
Thr Thr Pro Leu Met Leu Pro His Lys Ala Thr Thr Arg Val Lys Ile
385 390 395 400
Gly Gly Tyr Asp Ile Pro Lys Gly Thr Ile Val His Val Asn Val Tyr
405 410 415
Ala Ile Gly Arg Asp Pro Lys Val Trp Lys Arg Ala Ser Val Phe Arg
420 425 430
Pro Glu Arg Phe Leu Glu Glu Asp Val Asp Ile Lys Gly His Asp Tyr
435 440 445
Arg Leu Leu Pro Phe Gly Ala Gly Arg Arg Ile Cys Pro Gly Ala Gln
450 455 460
Leu Gly Leu Asn Met Val Gln Leu Met Val Ala Arg Leu Leu His Gln
465 470 475 480
Phe Ser Trp Ala Pro Pro Pro Gly Val Lys Pro Glu Lys Ile Asp Leu
485 490 495
Thr Glu Arg Pro Gly Val Val Thr Phe Met Ala Asn Pro Val Gln Ala
500 505 510
Val Ala Thr Pro Arg Leu Ala Glu Lys Leu Tyr Glu
515 520
<210> 190
<211> 487
<212> PRT
<213> 小立碗藓()
<220>
<223> 藓豆酰基莽草酸3-羟化酶 (FpC3H)
<400> 190
Met Tyr Lys Ser Leu Arg Ser Ser His Lys Leu Pro Pro Gly Pro Arg
1 5 10 15
Pro Leu Pro Val Val Gly Asn Leu Thr His Ile Thr Pro Val Arg Phe
20 25 30
Lys Cys Phe Met Glu Trp Ala Gln Thr Tyr Gly Ser Val Leu Ser Val
35 40 45
Trp Met Gly Pro Thr Leu Asn Val Val Val Ser Ser Ala Asp Ala Ala
50 55 60
Lys Glu Met Leu Lys Glu Arg Asp His Ala Leu Ser Ser Arg Pro Leu
65 70 75 80
Thr Arg Ala Ala Ala Arg Phe Ser Arg Asn Gly Gln Asp Leu Ile Trp
85 90 95
Ala Asp Tyr Gly Pro His Tyr Val Lys Val Arg Lys Val Cys Thr Leu
100 105 110
Glu Leu Phe Thr Phe Lys Arg Leu Glu Ser Leu Lys Pro Val Arg Glu
115 120 125
Asp Glu Val Gly Ala Met Val Ala Ala Leu Phe Lys Asp Cys Ala Asp
130 135 140
Ser Arg Pro Leu Asn Leu Lys Lys Tyr Val Ser Ala Met Ala Phe Asn
145 150 155 160
Asn Ile Thr Arg Ile Val Phe Gly Lys Arg Phe Val Asp Asp Lys Gly
165 170 175
Asn Ile Asp Asn Gln Gly Val Glu Phe Lys Glu Ile Val Ser Gln Gly
180 185 190
Met Lys Leu Gly Ala Ser Leu Lys Met Ser Glu His Ile Pro Tyr Leu
195 200 205
Arg Trp Met Phe Pro Leu Gln Glu Glu Glu Phe Ala Lys His Gly Ala
210 215 220
Arg Arg Asp Asn Leu Thr Lys Ala Ile Met Gln Glu His Arg Leu Gln
225 230 235 240
Ser Gln Lys Asn Gly Pro Gly His His Phe Val Asp Ala Leu Leu Ser
245 250 255
Met Gln Lys Gln Tyr Asp Leu Ser Glu Thr Thr Ile Ile Gly Leu Leu
260 265 270
Trp Asp Met Ile Thr Ala Gly Met Asp Thr Thr Ala Ile Ser Val Glu
275 280 285
Trp Ala Ile Ala Glu Leu Val Arg Asn Pro Asp Val Gln Val Lys Ala
290 295 300
Gln Gln Glu Leu Asp Gln Val Val Gly Gln Asp Arg Val Val Thr Glu
305 310 315 320
Ala Asp Phe Ser Gln Leu Pro Tyr Leu Gln Ala Val Ala Lys Glu Ala
325 330 335
Leu Arg Leu His Pro Pro Thr Pro Leu Met Leu Pro His Lys Ala Thr
340 345 350
Glu Thr Val Lys Ile Gly Gly Tyr Asp Val Pro Lys Gly Thr Val Val
355 360 365
His Cys Asn Val Tyr Ala Ile Ser Arg Asp Pro Thr Val Trp Glu Glu
370 375 380
Pro Leu Arg Phe Arg Pro Glu Arg Phe Leu Glu Glu Asp Ile Asp Ile
385 390 395 400
Lys Gly His Asp Tyr Arg Leu Leu Pro Phe Gly Ala Gly Arg Arg Val
405 410 415
Cys Pro Gly Ala Gln Leu Gly Leu Asn Met Val Gln Leu Met Leu Ala
420 425 430
Arg Leu Leu His His Phe Ser Trp Ala Pro Pro Pro Gly Val Thr Pro
435 440 445
Ala Ala Ile Asp Met Thr Glu Arg Pro Gly Val Val Thr Phe Met Ala
450 455 460
Ala Pro Leu Gln Val Leu Ala Thr Pro Arg Leu Arg Ala Ala Leu Tyr
465 470 475 480
Lys Asn Gly Ser Ser Pro Ser
485
<210> 191
<211> 338
<212> PRT
<213> 人工序列()
<220>
<223> 合成的肉桂酰基辅酶A还原酶 (CCR) 共有 序列（大多数）
<220>
<221> 变体
<222> (7)..(.7)
<223> Xaa = Val, Pro, Ser, Lys, Ala或Glu
<220>
<221> 变体
<222> (8)..(.8)
<223> Xaa = Ala, Pro, Leu, Ser, Gln或Val
<220>
<221> 变体
<222> (9)..(.9)
<223> Xaa = Ser, Thr, Val, Pro或Lys
<220>
<221> 变体
<222> (40)..(.40)
<223> Xaa = Lys, Arg或His
<220>
<221> 变体
<222> (79)..(.79)
<223> Xaa = Ala, Glu, Asp, Ser, Asn或Arg
<220>
<221> 变体
<222> (85)..(.85)
<223> Xaa = Asp, His或Gln
<220>
<221> 变体
<222> (114)..(.114)
<223> Xaa = Asn, Thr, Val, Ile, Asp, Ser或Lys
<220>
<221> 变体
<222> (179)..(.181)
<223> Xaa = Lys, Arg或Asn
<220>
<221> 变体
<222> (223)..(.223)
<223> Xaa = Leu, Ser, Tyr, Ala或His
<220>
<221> 变体
<222> (331)..(.331)
<223> Xaa = Glu, Asp或Gln
<220>
<221> 变体
<222> (337)..(.337)
<223> Xaa = Gly, Gln, His, Lys或Arg
<220>
<221> UNSURE
<222> (7)..(7)
<223> The 'Xaa' at location 7 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (8)..(8)
<223> The 'Xaa' at location 8 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (9)..(9)
<223> The 'Xaa' at location 9 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (40)..(40)
<223> The 'Xaa' at location 40 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (79)..(79)
<223> The 'Xaa' at location 79 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (85)..(85)
<223> The 'Xaa' at location 85 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (114)..(114)
<223> The 'Xaa' at location 114 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (179)..(179)
<223> The 'Xaa' at location 179 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (181)..(181)
<223> The 'Xaa' at location 181 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (223)..(223)
<223> The 'Xaa' at location 223 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (331)..(331)
<223> The 'Xaa' at location 331 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (337)..(337)
<223> The 'Xaa' at location 337 stands for Gln, Arg, Pro, or Leu.
<400> 191
Met Thr Val Asp Ala Ala Xaa Xaa Xaa Gly Asn Gly Gln Thr Val Cys
1 5 10 15
Val Thr Gly Ala Gly Gly Phe Ile Ala Ser Trp Leu Val Lys Leu Leu
20 25 30
Leu Glu Lys Gly Tyr Thr Val Xaa Gly Thr Val Arg Asn Pro Asp Asp
35 40 45
Pro Lys Asn Ala His Leu Arg Glu Leu Glu Gly Ala Ala Glu Arg Leu
50 55 60
Thr Leu Cys Lys Ala Asp Leu Leu Asp Tyr Asp Ser Leu Cys Xaa Ala
65 70 75 80
Ile Gln Gly Cys Xaa Gly Val Phe His Thr Ala Ser Pro Val Thr Asp
85 90 95
Asp Pro Glu Gln Met Val Glu Pro Ala Val Arg Gly Thr Lys Asn Val
100 105 110
Ile Xaa Ala Ala Ala Glu Ala Lys Val Arg Arg Val Val Phe Thr Ser
115 120 125
Ser Ile Gly Ala Val Tyr Met Asp Pro Asn Arg Gly Pro Asp Val Val
130 135 140
Val Asp Glu Ser Cys Trp Ser Asp Leu Glu Phe Cys Lys Asn Thr Lys
145 150 155 160
Asn Trp Tyr Cys Tyr Gly Lys Ala Val Ala Glu Gln Ala Ala Trp Glu
165 170 175
Ala Ala Xaa Glu Xaa Gly Val Asp Leu Val Val Val Asn Pro Val Leu
180 185 190
Val Leu Gly Pro Leu Leu Gln Pro Thr Val Asn Ala Ser Ile Ala His
195 200 205
Ile Leu Lys Tyr Leu Thr Gly Ser Ala Lys Thr Tyr Ala Asn Xaa Val
210 215 220
Gln Ala Tyr Val His Val Arg Asp Val Ala Leu Ala His Ile Leu Val
225 230 235 240
Phe Glu Ser Pro Ser Ala Ser Gly Arg Tyr Leu Cys Ala Glu Ser Val
245 250 255
Leu His Arg Gly Asp Val Val Glu Ile Leu Ala Lys Leu Phe Pro Glu
260 265 270
Tyr Pro Val Pro Thr Lys Cys Ser Asp Glu Val Asn Pro Arg Lys Lys
275 280 285
Pro Tyr Lys Phe Ser Asn Gln Lys Leu Arg Asp Leu Gly Leu Glu Phe
290 295 300
Thr Pro Val Lys Gln Cys Leu Tyr Glu Thr Val Lys Ser Leu Gln Glu
305 310 315 320
Lys Gly His Leu Pro Val Pro Ala Gln Gln Xaa Gly Gly Ile Ala Ile
325 330 335
Xaa Ala
<210> 192
<211> 332
<212> PRT
<213> 番茄()
<220>
<223> 番茄肉桂酰基辅酶A还原酶 (SlCCR)
<400> 192
Met Pro Ser Val Ser Gly Arg Val Val Cys Val Thr Gly Ala Gly Gly
1 5 10 15
Phe Ile Ala Ser Trp Leu Val Lys Leu Leu Leu Glu Lys Gly Tyr Thr
20 25 30
Val Arg Gly Thr Val Arg Asn Pro Asp Asp Pro Lys Asn Cys His Leu
35 40 45
Arg Glu Leu Glu Gly Ala Lys Glu Arg Leu Thr Leu Cys Arg Gly Asp
50 55 60
Leu Leu Asp Tyr Gln Ser Leu Arg Glu Ala Ile Asn Gly Cys Asp Gly
65 70 75 80
Val Phe His Thr Ala Ser Pro Val Thr Asp Asp Pro Glu Gln Met Val
85 90 95
Glu Pro Ala Val Ile Gly Thr Lys Asn Val Ile Thr Ala Ala Ala Glu
100 105 110
Ala Asn Val Arg Arg Val Val Phe Thr Ser Ser Ile Gly Ala Val Tyr
115 120 125
Met Asp Pro Ser Arg Asp Pro Glu Lys Val Val Asp Glu Thr Cys Trp
130 135 140
Ser Asp Pro Asp Phe Cys Lys Asn Thr Lys Asn Trp Tyr Cys Tyr Gly
145 150 155 160
Lys Met Val Ala Glu Gln Ala Ala Trp Asp Glu Ala Arg Glu Lys Gly
165 170 175
Val Asp Leu Val Ala Ile Asn Pro Val Leu Val Leu Gly Pro Leu Leu
180 185 190
Gln Asn Thr Val Asn Ala Ser Val Leu His Ile Leu Lys Tyr Leu Thr
195 200 205
Gly Ser Ala Lys Thr Tyr Ala Asn Ser Val Gln Ala Tyr Val His Val
210 215 220
Lys Asp Val Ala Leu Ala His Ile Leu Leu Tyr Glu Thr Pro Ser Ala
225 230 235 240
Ser Gly Arg Tyr Leu Cys Ala Glu Ser Val Leu His Arg Gly Asp Ile
245 250 255
Val Glu Ile Leu Ala Lys Phe Phe Pro Glu Tyr Pro Ile Pro Thr Lys
260 265 270
Cys Ser Asp Val Thr Lys Pro Arg Val Lys Pro Tyr Lys Phe Ser Asn
275 280 285
Gln Lys Leu Lys Asp Leu Gly Met Glu Phe Thr Pro Val Lys Gln Cys
290 295 300
Leu Tyr Glu Thr Val Lys Ser Leu Gln Glu Lys Gly His Leu Pro Ile
305 310 315 320
Pro Thr Gln Lys Asp Glu Ile Ile Arg Ile Gln Thr
325 330
<210> 193
<211> 336
<212> PRT
<213> 一品红()
<220>
<223> 一品红肉桂酰基辅酶A还原酶 (EpCCR)
<400> 193
Met Pro Val Asp Ala Leu Pro Gly Ser Gly Gln Thr Val Cys Val Thr
1 5 10 15
Gly Ala Gly Gly Phe Ile Ala Ser Trp Ile Val Lys Leu Phe Leu Glu
20 25 30
Arg Gly Tyr Thr Val Arg Gly Thr Val Arg Asn Pro Asp Asp Pro Lys
35 40 45
Asn Gly His Leu Arg Glu Leu Glu Gly Ala Ser Glu Arg Leu Thr Leu
50 55 60
Tyr Lys Gly Asp Leu Met Asp Tyr Glu Ser Leu Arg Glu Ala Ile Met
65 70 75 80
Gly Cys Asp Gly Val Val His Thr Ala Ser Pro Val Thr Asp Asp Pro
85 90 95
Glu Gln Met Val Glu Pro Ala Val Ile Gly Thr Lys Asn Val Ile Val
100 105 110
Ala Ala Ala Glu Ala Lys Val Arg Arg Val Val Phe Thr Ser Ser Val
115 120 125
Gly Ala Ile Thr Met Asp Pro Asn Arg Gly Pro Asp Val Val Val Asp
130 135 140
Glu Ser Cys Trp Ser Asp Leu Asp Phe Cys Lys Ser Thr Lys Asn Trp
145 150 155 160
Tyr Cys Tyr Gly Lys Ala Val Ala Glu Lys Ser Ala Cys Ala Glu Ala
165 170 175
Lys Glu Arg Gly Val Asp Leu Val Val Ile Asn Pro Val Leu Val Leu
180 185 190
Gly Pro Leu Leu Gln Ser Thr Val Asn Ala Ser Ile Ile His Ile Leu
195 200 205
Lys Tyr Leu Thr Gly Ser Ala Lys Thr Tyr Ala Asn Ser Val Gln Ala
210 215 220
Tyr Val His Val Lys Asp Val Ala Leu Ala His Ile Leu Val Phe Glu
225 230 235 240
Thr Pro Ser Ala Ser Gly Arg Tyr Leu Cys Ala Glu Ser Val Leu His
245 250 255
Arg Gly Asp Val Val Glu Ile Leu Ala Lys Phe Phe Pro Glu Tyr Asn
260 265 270
Leu Pro Thr Lys Cys Ser Asp Glu Val Asn Pro Arg Val Lys Pro Tyr
275 280 285
Lys Phe Ser Asn Gln Lys Leu Arg Asp Leu Gly Leu Glu Phe Thr Pro
290 295 300
Val Lys Gln Cys Leu Tyr Glu Thr Val Lys Ser Leu Gln Glu Arg Gly
305 310 315 320
His Leu Ala Val Pro Ser Pro Pro Glu Asp Ser Val Arg Ile Gln Arg
325 330 335
<210> 194
<211> 331
<212> PRT
<213> 马铃薯()
<220>
<223> 马铃薯肉桂酰基辅酶A还原酶 (StCCR)
<400> 194
Met Pro Ser Glu Ser Gly Lys Val Val Cys Val Thr Gly Ala Gly Gly
1 5 10 15
Phe Ile Ala Ser Trp Leu Val Lys Leu Leu Leu Glu Lys Gly Tyr Thr
20 25 30
Val Arg Gly Thr Val Arg Asn Pro Asp Asp Pro Lys Asn Gly His Leu
35 40 45
Lys Glu Leu Glu Gly Lys Glu Arg Leu Ile Leu Leu Arg Ala Asp Leu
50 55 60
Leu Asp Tyr Gln Ser Leu Arg Glu Ala Ile Tyr Gly Cys Asp Gly Val
65 70 75 80
Phe His Thr Ala Ser Pro Val Thr Asp Asp Pro Glu Gln Met Val Glu
85 90 95
Pro Ala Val Ile Gly Thr Lys Asn Val Ile Thr Ala Ala Ala Glu Ala
100 105 110
Lys Val Gly Arg Val Val Phe Thr Ser Ser Ile Gly Thr Val Tyr Met
115 120 125
Asp Pro Asn Arg Ala Pro Asp Lys Val Val Asp Glu Thr Cys Trp Ser
130 135 140
Asp Leu Gly Phe Cys Lys Asn Thr Lys Asn Trp Tyr Cys Tyr Gly Lys
145 150 155 160
Thr Val Ala Glu Lys Thr Ala Trp Asp Glu Ala Arg Glu Lys Gly Val
165 170 175
Asp Leu Val Val Ile Asn Pro Val Leu Val Leu Gly Pro Leu Leu Gln
180 185 190
Pro Thr Val Asn Ala Ser Val Leu His Ile Leu Lys Tyr Leu Thr Gly
195 200 205
Ser Ala Lys Thr Tyr Ala Asn Ser Ile Gln Ala Tyr Val His Val Lys
210 215 220
Asp Val Ala Leu Ala His Ile Leu Leu Tyr Glu Ala Pro Ser Ala Ser
225 230 235 240
Gly Arg Tyr Ile Cys Ala Glu Ser Val Leu His Arg Gly Asp Val Val
245 250 255
Glu Ile Leu Ala Lys Phe Phe Pro Glu Tyr Pro Ile Pro Thr Lys Cys
260 265 270
Ser Asp Glu Thr Arg Pro Arg Ala Lys Pro Tyr Lys Phe Thr Asn Gln
275 280 285
Lys Leu Lys Asp Leu Gly Leu Gly Phe Thr Pro Val Lys Gln Cys Leu
290 295 300
Tyr Glu Thr Val Lys Ser Leu Gln Glu Lys Gly His Leu Pro Ile Pro
305 310 315 320
Thr Gln Asn Asp Glu Pro Ile Lys Ile His Ser
325 330
<210> 195
<211> 336
<212> PRT
<213> 冈尼桉()
<220>
<223> 肉桂酰基辅酶A还原酶 (EgCCR)
<400> 195
Met Pro Val Asp Ala Leu Pro Gly Ser Gly Gln Thr Val Cys Val Thr
1 5 10 15
Gly Ala Gly Gly Phe Ile Ala Ser Trp Ile Val Lys Leu Leu Leu Glu
20 25 30
Arg Gly Tyr Thr Val Arg Gly Thr Val Arg Asn Pro Asp Asp Pro Lys
35 40 45
Asn Gly His Leu Arg Glu Leu Glu Gly Ala Ser Glu Arg Leu Thr Leu
50 55 60
Tyr Lys Gly Asp Leu Met Asp Tyr Gly Ser Leu Glu Glu Ala Ile Lys
65 70 75 80
Gly Cys Asp Gly Val Val His Thr Ala Ser Pro Val Thr Asp Asp Pro
85 90 95
Glu Gln Met Val Glu Pro Ala Val Ile Gly Thr Lys Asn Val Ile Val
100 105 110
Ala Ala Ala Glu Ala Lys Val Arg Arg Val Val Phe Thr Ser Ser Ile
115 120 125
Gly Ala Val Thr Met Asp Pro Asn Arg Gly Pro Asp Val Val Val Asp
130 135 140
Glu Ser Cys Trp Ser Asp Leu Glu Phe Cys Lys Ser Thr Lys Asn Trp
145 150 155 160
Tyr Cys Tyr Gly Lys Ala Val Ala Glu Lys Ala Ala Trp Pro Glu Ala
165 170 175
Lys Glu Arg Gly Val Asp Leu Val Val Ile Asn Pro Val Leu Val Leu
180 185 190
Gly Pro Leu Leu Gln Ser Thr Ile Asn Ala Ser Ile Ile His Ile Leu
195 200 205
Lys Tyr Leu Thr Gly Ser Ala Lys Thr Tyr Ala Asn Ser Val Gln Ala
210 215 220
Tyr Val His Val Lys Asp Val Ala Leu Ala His Val Leu Val Leu Glu
225 230 235 240
Thr Pro Ser Ala Ser Gly Arg Tyr Leu Cys Ala Glu Ser Val Leu His
245 250 255
Arg Gly Asp Val Val Glu Ile Leu Ala Lys Phe Phe Pro Glu Tyr Asn
260 265 270
Val Pro Thr Lys Cys Ser Asp Glu Val Asn Pro Arg Val Lys Pro Tyr
275 280 285
Lys Phe Ser Asn Gln Lys Leu Arg Asp Leu Gly Leu Glu Phe Thr Pro
290 295 300
Val Lys Gln Cys Leu Tyr Glu Thr Val Lys Ser Leu Gln Glu Lys Gly
305 310 315 320
His Leu Pro Val Pro Ser Pro Pro Glu Asp Ser Val Arg Ile Gln Gly
325 330 335
<210> 196
<211> 338
<212> PRT
<213> 葡萄()
<220>
<223> 葡萄肉桂酰基辅酶A还原酶 (VvCCR)
<400> 196
Met Pro Ile Asp Ala Ser Ser Pro Ser Ser Ser Gly Gln Ile Val Cys
1 5 10 15
Val Thr Gly Ala Gly Gly Phe Ile Ala Ser Trp Ile Val Lys Leu Leu
20 25 30
Leu Glu Arg Gly Tyr Thr Val Lys Gly Thr Val Arg Asn Pro Asp Asp
35 40 45
Pro Lys Asn Ser His Leu Arg Glu Leu Glu Gly Ala Lys Glu Arg Leu
50 55 60
Ile Leu Cys Lys Ala Asp Leu Met Asp Tyr Glu Ser Leu Lys Glu Ala
65 70 75 80
Ile Asn Gly Cys His Gly Val Phe His Thr Ala Ser Pro Val Thr Asp
85 90 95
Asp Pro Glu Glu Met Val Glu Pro Ala Val Asn Gly Thr Lys Asn Val
100 105 110
Ile Ile Ala Ala Ala Glu Ala Lys Val Arg Arg Val Val Phe Thr Ser
115 120 125
Ser Ile Gly Ala Val Tyr Met Asp Pro Asn Arg Ser Pro Asp Gln Val
130 135 140
Val Asp Glu Ser Cys Trp Ser Asp Leu Glu Phe Cys Lys Asn Thr Lys
145 150 155 160
Asn Trp Tyr Cys Tyr Gly Lys Ala Val Ala Glu Gln Ala Ala Trp Glu
165 170 175
Val Ala Lys Glu Lys Glu Val Asp Leu Val Val Val Asn Pro Val Leu
180 185 190
Val Leu Gly Pro Leu Leu Gln Ser Thr Val Asn Ala Ser Ile Ile His
195 200 205
Ile Leu Lys Tyr Leu Thr Gly Ser Ala Lys Thr Tyr Ala Asn Ser Val
210 215 220
Gln Ala Tyr Val His Val Lys Asp Val Ala Leu Ala His Ile Leu Val
225 230 235 240
Phe Glu Ala Pro Ser Ala Ser Gly Arg Tyr Leu Cys Ala Glu Ser Val
245 250 255
Leu His Arg Gly Asp Val Val Glu Ile Leu Ala Lys Phe Phe Pro Glu
260 265 270
Tyr Pro Ile Pro Thr Lys Cys Ser Asp Glu Val Lys Pro Arg Ala Lys
275 280 285
Pro Tyr Lys Phe Ser Asn Gln Lys Leu Arg Glu Leu Gly Leu Glu Phe
290 295 300
Ile Pro Val Lys Gln Cys Leu Tyr Glu Thr Val Lys Thr Leu Gln Glu
305 310 315 320
Lys Gly His Val Pro Val Pro Pro Gln His Asp Asp Ser Leu Arg Ile
325 330 335
Gln Ser
<210> 197
<211> 339
<212> PRT
<213> 蓖麻()
<220>
<223> 肉桂酰基辅酶A还原酶 (RcCCR)
<400> 197
Met Pro Ala Ala Asp Thr Ser Ser Leu Ser Gly His Gly Gln Thr Val
1 5 10 15
Cys Val Thr Gly Ala Gly Gly Phe Ile Ala Ser Trp Ile Val Lys Leu
20 25 30
Leu Leu Glu Lys Gly Tyr Thr Val Arg Gly Thr Val Arg Asn Pro Asp
35 40 45
Asp Pro Lys Asn Val His Leu Arg Glu Leu Glu Gly Ala Asn Glu Arg
50 55 60
Leu Thr Leu Cys Arg Ala Asp Leu Leu Asp Phe Gln Ser Leu Arg Asp
65 70 75 80
Ala Ile Met Gly Cys Asp Gly Val Phe His Thr Ala Ser Pro Val Thr
85 90 95
Asp Asp Pro Glu Gln Met Val Glu Pro Ala Val Asn Gly Thr Lys Asn
100 105 110
Val Ile Ile Ala Ala Ala Glu Ala Lys Val Arg Arg Val Val Phe Thr
115 120 125
Ser Ser Ile Gly Ala Val Tyr Met Asp Pro Asn Arg Gly Pro Asp Val
130 135 140
Val Val Asp Glu Ser Cys Trp Ser Asp Leu Glu Phe Cys Lys Asn Thr
145 150 155 160
Lys Asn Trp Tyr Cys Tyr Gly Lys Ala Val Ala Glu Gln Ala Ala Trp
165 170 175
Glu Glu Ala Lys Glu Lys Gly Leu Asp Leu Val Val Val Asn Pro Val
180 185 190
Leu Val Leu Gly Pro Leu Leu Gln Pro Thr Ile Asn Ala Ser Ile Ile
195 200 205
His Ile Leu Lys Tyr Leu Thr Gly Ser Ala Lys Thr Tyr Ala Asn Tyr
210 215 220
Val Gln Ala Tyr Val His Val Lys Asp Val Ala Leu Ala His Ile Leu
225 230 235 240
Val Tyr Glu Thr Pro Ser Ala Ser Gly Arg Tyr Leu Cys Ala Glu Ser
245 250 255
Val Leu His Arg Gly Glu Val Val Glu Ile Leu Ala Lys Phe Phe Pro
260 265 270
Glu Tyr Pro Ile Pro Thr Lys Cys Ser Asp Glu Lys Asn Pro Arg Ala
275 280 285
Lys Pro Tyr Lys Phe Ser Asn Gln Lys Leu Lys Asp Leu Gly Leu Glu
290 295 300
Phe Thr Pro Val Lys Gln Cys Leu Tyr Glu Thr Val Arg Asn Leu Gln
305 310 315 320
Glu Arg Gly His Leu Pro Ile Pro Lys Gln Ala Glu Asp Ser Val Arg
325 330 335
Ile Gln Ala
<210> 198
<211> 338
<212> PRT
<213> 火炬松()
<220>
<223> 松树肉桂酰基辅酶A还原酶 (PtCCR)
<400> 198
Met Pro Val Asp Ala Ser Ser Leu Ser Gly Gln Gly Gln Thr Ile Cys
1 5 10 15
Val Thr Gly Ala Gly Gly Phe Ile Ala Ser Trp Met Val Lys Leu Leu
20 25 30
Leu Asp Lys Gly Tyr Thr Val Arg Gly Thr Ala Arg Asn Pro Ala Asp
35 40 45
Pro Lys Asn Ser His Leu Arg Glu Leu Glu Gly Ala Gln Glu Arg Leu
50 55 60
Thr Leu Cys Lys Ala Asp Leu Leu Asp Tyr Glu Ser Leu Lys Glu Ala
65 70 75 80
Ile Gln Gly Cys Asp Gly Val Phe His Thr Ala Ser Pro Val Thr Asp
85 90 95
Asp Pro Glu Glu Met Val Glu Pro Ala Val Asn Gly Thr Lys Asn Val
100 105 110
Ile Ile Ala Ala Ala Glu Ala Lys Val Arg Arg Val Val Phe Thr Ser
115 120 125
Ser Ile Gly Ala Val Tyr Met Asp Pro Asn Lys Gly Pro Asp Val Val
130 135 140
Ile Asp Glu Ser Cys Trp Ser Asp Leu Glu Phe Cys Lys Asn Thr Lys
145 150 155 160
Asn Trp Tyr Cys Tyr Gly Lys Ala Val Ala Glu Gln Ala Ala Trp Asp
165 170 175
Met Ala Lys Glu Lys Gly Val Asp Leu Val Val Val Asn Pro Val Leu
180 185 190
Val Leu Gly Pro Leu Leu Gln Pro Thr Val Asn Ala Ser Ile Val His
195 200 205
Ile Leu Lys Tyr Leu Thr Gly Ser Ala Lys Thr Tyr Ala Asn Ser Val
210 215 220
Gln Ala Tyr Val His Val Arg Asp Val Ala Leu Ala His Ile Leu Val
225 230 235 240
Phe Glu Thr Pro Ser Ala Ser Gly Arg Tyr Leu Cys Ser Glu Ser Val
245 250 255
Leu His Arg Gly Glu Val Val Glu Ile Leu Ala Lys Phe Phe Pro Glu
260 265 270
Tyr Pro Ile Pro Thr Lys Cys Ser Asp Glu Lys Asn Pro Arg Lys Gln
275 280 285
Pro Tyr Lys Phe Ser Asn Gln Lys Leu Arg Asp Leu Gly Phe Glu Phe
290 295 300
Thr Pro Val Lys Gln Cys Leu Tyr Glu Thr Val Lys Ser Leu Gln Glu
305 310 315 320
Arg Gly His Leu Pro Ile Pro Lys Gln Ala Ala Glu Glu Ser Val Lys
325 330 335
Ile Gln
<210> 199
<211> 338
<212> PRT
<213> 火炬松()
<220>
<223> 松树肉桂酰基辅酶A还原酶 (PtCCR)
<400> 199
Met Pro Val Asp Ala Ser Ser Leu Ser Gly Gln Gly Gln Thr Ile Cys
1 5 10 15
Val Thr Gly Gly Gly Gly Phe Ile Ala Ser Trp Met Val Lys Leu Leu
20 25 30
Leu Asp Lys Gly Tyr Thr Val Arg Gly Thr Ala Arg Asn Pro Ala Asp
35 40 45
Pro Lys Asn Ser His Leu Arg Glu Leu Glu Gly Ala Glu Glu Arg Leu
50 55 60
Thr Leu Cys Lys Ala Asp Leu Leu Asp Tyr Glu Ser Leu Lys Glu Gly
65 70 75 80
Ile Gln Gly Cys Asp Gly Val Phe His Thr Ala Ser Pro Val Thr Asp
85 90 95
Asp Pro Glu Glu Met Val Glu Pro Ala Val Asn Gly Thr Lys Asn Val
100 105 110
Ile Ile Ala Ala Ala Glu Ala Lys Val Arg Arg Val Val Phe Thr Ser
115 120 125
Ser Ile Gly Ala Val Tyr Met Asp Pro Asn Lys Gly Pro Asp Val Val
130 135 140
Ile Asp Glu Ser Cys Trp Ser Asp Leu Glu Phe Cys Lys Asn Thr Lys
145 150 155 160
Asn Trp Tyr Cys Tyr Gly Lys Ala Val Ala Glu Gln Ala Ala Trp Asp
165 170 175
Met Ala Lys Glu Lys Gly Val Asp Leu Val Val Val Asn Pro Val Leu
180 185 190
Val Leu Gly Pro Leu Leu Gln Pro Thr Val Asn Ala Ser Ile Thr His
195 200 205
Ile Leu Lys Tyr Leu Thr Gly Ser Ala Lys Thr Tyr Ala Asn Ser Val
210 215 220
Gln Ala Tyr Val His Val Arg Asp Val Ala Leu Ala His Ile Leu Val
225 230 235 240
Phe Glu Thr Pro Ser Ala Ser Gly Arg Tyr Leu Cys Ser Glu Ser Val
245 250 255
Leu His Arg Gly Glu Val Val Glu Ile Leu Ala Lys Phe Phe Pro Glu
260 265 270
Tyr Pro Ile Pro Thr Lys Cys Ser Asp Glu Lys Asn Pro Arg Lys Gln
275 280 285
Pro Tyr Lys Phe Ser Asn Gln Lys Leu Arg Asp Leu Gly Phe Glu Phe
290 295 300
Thr Pro Val Lys Gln Cys Leu Tyr Glu Thr Val Lys Ser Leu Gln Glu
305 310 315 320
Lys Gly His Leu Pro Ile Pro Lys Gln Ala Ala Glu Glu Ser Leu Lys
325 330 335
Ile Gln
<210> 200
<211> 338
<212> PRT
<213> 大豆()
<220>
<223> 大豆肉桂酰基辅酶A还原酶 (GmCCR)
<400> 200
Met Pro Ser Ser Glu Ser Ser Thr Gly Phe Ser Glu Thr Ile Cys Val
1 5 10 15
Thr Gly Ala Gly Gly Phe Ile Ala Ser Trp Met Val Lys Leu Leu Leu
20 25 30
Glu Lys Gly Tyr Thr Val Arg Gly Thr Leu Arg Asn Pro Asp Asp Pro
35 40 45
Lys Asn Gly His Leu Lys Glu Phe Glu Gly Ala Ser Gln Arg Leu Thr
50 55 60
Leu His Lys Val Asp Leu Leu His Leu Asp Ser Val Arg Ser Val Ile
65 70 75 80
Asn Gly Cys His Gly Val Phe His Thr Ala Ser Pro Val Thr Asp Asn
85 90 95
Pro Glu Glu Met Val Glu Pro Ala Val Asn Gly Ala Lys Asn Val Ile
100 105 110
Ile Ala Ala Ala Glu Ala Lys Val Arg Arg Val Val Phe Thr Ser Ser
115 120 125
Ile Gly Ala Val Tyr Met Asp Pro Lys Arg Ser Ile Asp Leu Val Val
130 135 140
Asp Glu Ser Cys Trp Ser Asp Leu Glu Phe Cys Met Asn Thr Lys Asn
145 150 155 160
Trp Tyr Cys Tyr Gly Lys Ala Val Ala Glu Glu Ala Ala Trp Asp Thr
165 170 175
Ala Lys Glu Lys Gly Val Asp Met Val Val Val Asn Pro Val Leu Val
180 185 190
Leu Gly Pro Leu Leu Gln Pro Ser Ile Asn Ala Ser Thr Ile His Ile
195 200 205
Leu Lys Tyr Leu Thr Gly Phe Gly Lys Thr Tyr Ala Asn Ala Thr Gln
210 215 220
Gly Tyr Val His Val Arg Asp Val Ala Leu Ala His Ile Leu Val Tyr
225 230 235 240
Glu Lys Pro Ser Ala Phe Gly Arg Tyr Ile Cys Ala Glu Ser Ser Phe
245 250 255
His Arg Gly Glu Leu Val Glu Ile Leu Ala Lys Tyr Phe Pro Asp Tyr
260 265 270
Pro Val Pro Thr Lys Cys Ser Asp Glu Lys Asn Pro Arg Ala Lys Pro
275 280 285
Tyr Thr Phe Ser Asn Gln Lys Met Lys Asp Leu Gly Leu Glu Phe Pro
290 295 300
Pro Val Ser Gln Cys Leu Tyr Glu Ala Val Lys Asn Leu Gln Glu Lys
305 310 315 320
Gly His Leu Pro Val Pro Ala Arg Gln Gln Glu Asp Ser Thr Thr Val
325 330 335
Lys Pro
<210> 201
<211> 322
<212> PRT
<213> 挪威云杉()
<220>
<223> 云杉肉桂酰基辅酶A还原酶 (PaCCR)
<400> 201
Met Thr Ala Gly Lys Gln Thr Gly Ala Gly Gln Thr Val Cys Val Thr
1 5 10 15
Gly Ala Gly Gly Phe Ile Ala Ser Trp Leu Val Lys Leu Leu Leu Glu
20 25 30
Arg Gly Tyr Thr Val Arg Gly Thr Val Arg Asn Pro Glu Asp Gln Lys
35 40 45
Asn Ala His Leu Arg Gln Leu Glu Gly Ala Glu Glu Arg Leu Thr Leu
50 55 60
Val Lys Ala Asp Leu Met Asp Tyr Asn Ser Leu Leu Asn Ala Ile Thr
65 70 75 80
Gly Cys Gln Gly Val Phe His Val Ala Ser Pro Val Thr Asp Asp Pro
85 90 95
Val Gln Met Val Glu Pro Ala Val Asn Gly Thr Lys Asn Val Leu Asp
100 105 110
Ala Cys Ala Glu Ala Ala Val Arg Arg Val Val Phe Thr Ser Ser Ile
115 120 125
Gly Ala Val Tyr Met Asp Pro Thr Arg Asp Tyr Asp Ala Leu Val Asp
130 135 140
Glu Ser Cys Trp Ser Asn Leu Asp Phe Cys Lys Asp Thr Lys Asn Trp
145 150 155 160
Tyr Cys Tyr Gly Lys Ala Val Ala Glu Lys Ala Ala Trp Asp Arg Ala
165 170 175
Lys Glu Lys Gly Leu Asp Leu Val Val Val Asn Pro Cys Val Val Leu
180 185 190
Gly Pro Val Leu Gln Ser Ser Ile Asn Ala Ser Ile Leu His Ile Leu
195 200 205
Lys Tyr Leu Thr Gly Ser Ala Lys Thr Tyr Ala Asn Ser Val Gln Ala
210 215 220
Tyr Val His Val Arg Asp Val Ala Glu Ala His Ile Leu Val Tyr Glu
225 230 235 240
Ser Pro Ser Ala Ser Gly Arg Tyr Leu Cys Ala Glu Ser Val Leu His
245 250 255
Arg Gly Asp Val Val Glu Leu Leu Glu Lys Met Phe Pro Gln Tyr Pro
260 265 270
Ile Pro Thr Lys Cys Lys Asp Asp Gly Lys Pro Arg Val Lys Pro Trp
275 280 285
Lys Val Ser Asn Gln Lys Leu Lys Asp Leu Gly Leu Glu Phe Thr Pro
290 295 300
Ala Lys Gln Cys Leu Tyr Glu Thr Val Ile Ser Leu Gln Glu Lys Gly
305 310 315 320
His Ile
<210> 202
<211> 324
<212> PRT
<213> 马尾松()
<220>
<223> 松树肉桂酰基辅酶A还原酶 (PmCCR)
<400> 202
Met Thr Ala Gly Lys Gln Thr Glu Glu Gly Gln Thr Gly Cys Val Thr
1 5 10 15
Gly Ala Gly Gly Phe Ile Ala Ser Trp Leu Val Lys Leu Leu Leu Glu
20 25 30
Arg Gly Tyr Thr Val Arg Gly Thr Val Arg Asn Pro Glu Asp Gln Lys
35 40 45
Asn Ala His Leu Lys Gln Leu Glu Gly Ala Glu Glu Arg Leu Thr Leu
50 55 60
Val Lys Ala Asp Leu Met Asp Tyr Asn Ser Leu Leu Asn Ala Ile Asn
65 70 75 80
Gly Cys Gln Gly Val Phe His Val Ala Ser Pro Val Thr Asp Asp Pro
85 90 95
Glu Glu Met Val Glu Pro Ala Val Asn Gly Thr Lys Asn Val Leu Asp
100 105 110
Ala Cys Ala Val Ala Gly Val Arg Arg Val Val Phe Thr Ser Ser Ile
115 120 125
Gly Ala Val Tyr Met Asp Pro Ser Arg Asp Tyr Asp Ala Leu Val Asp
130 135 140
Glu Asn Cys Trp Ser Asn Leu Asp Tyr Cys Lys Glu Thr Lys Asn Trp
145 150 155 160
Tyr Cys Tyr Gly Lys Thr Val Ala Glu Lys Ala Ala Trp Glu Arg Ala
165 170 175
Lys Asp Lys Gly Leu Asp Leu Val Val Val Asn Pro Cys Val Val Leu
180 185 190
Gly Pro Val Leu Gln Ser Ser Ile Asn Ala Ser Ile Ile His Ile Leu
195 200 205
Lys Tyr Leu Thr Gly Ser Ala Lys Thr Tyr Ala Asn Ser Val Gln Ala
210 215 220
Tyr Val His Val Arg Asp Val Ala Glu Ala His Ile Leu Val Tyr Glu
225 230 235 240
Ser Pro Ser Ala Ser Gly Arg Tyr Leu Cys Ala Glu Ser Val Leu His
245 250 255
Arg Gly Asp Val Val Asp Leu Leu Ala Ser Met Phe Pro Gln Tyr Pro
260 265 270
Ile Pro Thr Lys Val Lys Glu Asp Gly Lys Pro Arg Val Lys Pro Trp
275 280 285
Lys Val Ser Asn Gln Lys Leu Lys Asp Leu Gly Leu Glu Phe Thr Pro
290 295 300
Ala Lys Gln Cys Leu Tyr Glu Thr Val Ile Ser Leu Gln Glu Lys Gly
305 310 315 320
His Ile Ser Lys
<210> 203
<211> 361
<212> PRT
<213> 水稻()
<220>
<223> 稻肉桂酰基辅酶A还原酶 (OsCCR)
<400> 203
Met Thr Val Ile Asp Gly Ala Val Ala Ala Asp Ala Gly Gly Ala Ala
1 5 10 15
Ala Ala Val Val Gln Pro Gly Asn Gly Gln Thr Val Cys Val Thr Gly
20 25 30
Ala Ala Gly Tyr Ile Ala Ser Trp Leu Val Lys Leu Leu Leu Glu Lys
35 40 45
Gly Tyr Thr Val Lys Gly Thr Val Arg Asn Pro Asp Asp Pro Lys Asn
50 55 60
Ala His Leu Lys Ala Leu Asp Gly Ala Gly Glu Arg Leu Val Leu Cys
65 70 75 80
Lys Ala Asp Leu Leu Asp Tyr Asp Ala Ile Cys Arg Ala Val Ala Gly
85 90 95
Cys His Gly Val Phe His Thr Ala Ser Pro Val Thr Asp Asp Pro Glu
100 105 110
Gln Met Val Glu Pro Ala Val Arg Gly Thr Glu Tyr Val Ile Asn Ala
115 120 125
Ala Ala Glu Ala Gly Thr Val Arg Arg Val Val Phe Thr Ser Ser Ile
130 135 140
Gly Ala Val Thr Met Asp Pro Asn Arg Gly Pro Asp Val Val Val Asp
145 150 155 160
Glu Ser Cys Trp Ser Asp Leu Asp Tyr Cys Lys Glu Thr Arg Asn Trp
165 170 175
Tyr Cys Tyr Gly Lys Ala Val Ala Glu Gln Ala Ala Trp Glu Ala Ala
180 185 190
Arg Arg Arg Gly Val Glu Leu Val Val Val Asn Pro Val Leu Val Ile
195 200 205
Gly Pro Leu Leu Gln Pro Thr Val Asn Ala Ser Val Ala His Ile Leu
210 215 220
Lys Tyr Leu Asp Gly Ser Ala Ser Lys Phe Ala Asn Ala Val Gln Ala
225 230 235 240
Tyr Val Asp Val Arg Asp Val Ala Ala Ala His Leu Leu Val Phe Glu
245 250 255
Ser Pro Ser Ala Ala Gly Arg Phe Leu Cys Ala Glu Ser Val Leu His
260 265 270
Arg Glu Gly Val Val Arg Ile Leu Ala Lys Leu Phe Pro Glu Tyr Pro
275 280 285
Val Pro Thr Arg Cys Ser Asp Glu Lys Asn Pro Arg Lys Gln Pro Tyr
290 295 300
Lys Met Ser Asn Gln Lys Leu Arg Asp Leu Gly Leu Glu Phe Arg Pro
305 310 315 320
Ala Ser Gln Ser Leu Tyr Glu Thr Val Lys Cys Leu Gln Glu Lys Gly
325 330 335
His Leu Pro Val Leu Ala Ala Glu Lys Thr Glu Glu Glu Ala Gly Glu
340 345 350
Val Gln Gly Gly Ile Ala Ile Arg Ala
355 360
<210> 204
<211> 362
<212> PRT
<213> 黑麦草()
<220>
<223> 黑麦草肉桂酰基辅酶A还原酶 (LpCCR)
<400> 204
Met Thr Ile Ala Glu Val Val Ala Ala Gly Asp Thr Ala Ala Ala Val
1 5 10 15
Val Gln Pro Ala Gly Asn Gly Gln Thr Val Cys Val Thr Gly Ala Ala
20 25 30
Gly Tyr Ile Ala Ser Trp Leu Val Lys Leu Leu Leu Glu Lys Gly Tyr
35 40 45
Thr Val Lys Gly Thr Val Arg Asn Pro Asp Asp Pro Lys Asn Ala His
50 55 60
Leu Arg Ala Leu Asp Gly Ala Ala Asp Arg Leu Val Leu Cys Lys Ala
65 70 75 80
Asp Leu Leu Asp Tyr Asp Ala Ile Arg Arg Ala Ile Asp Gly Cys His
85 90 95
Gly Val Phe His Thr Ala Ser Pro Val Thr Asp Asp Pro Glu Gln Met
100 105 110
Val Glu Pro Ala Val Arg Gly Thr Gln Tyr Val Ile Asp Ala Ala Ala
115 120 125
Glu Ala Gly Thr Val Arg Arg Met Val Leu Thr Ser Ser Ile Gly Ala
130 135 140
Val Thr Met Asp Pro Asn Arg Gly Pro Asp Val Val Val Asp Glu Ser
145 150 155 160
Cys Trp Ser Asp Leu Asp Phe Cys Lys Lys Thr Arg Asn Trp Tyr Cys
165 170 175
Tyr Gly Lys Ala Val Ala Glu Gln Ala Ala Ser Glu Leu Ala Arg Gln
180 185 190
Arg Gly Val Asp Leu Val Val Val Asn Pro Val Leu Val Ile Gly Pro
195 200 205
Leu Leu Gln Pro Thr Val Asn Ala Ser Ile Gly His Ile Leu Lys Tyr
210 215 220
Leu Asp Gly Ser Ala Ser Lys Phe Ala Asn Ala Val Gln Ala Tyr Val
225 230 235 240
Asp Val Arg Asp Val Ala Asp Ala His Leu Arg Val Phe Glu Cys Ala
245 250 255
Ala Ala Ser Gly Arg His Leu Cys Ala Glu Arg Val Leu His Arg Glu
260 265 270
Asp Val Val Arg Ile Leu Ala Lys Leu Phe Pro Glu Tyr Pro Val Pro
275 280 285
Thr Arg Cys Ser Asp Glu Thr Asn Pro Arg Lys Gln Pro Tyr Lys Met
290 295 300
Ser Asn Gln Lys Leu Gln Asp Leu Gly Leu Glu Phe Arg Pro Val Ser
305 310 315 320
Gln Ser Leu Tyr Glu Thr Val Lys Ser Leu Gln Glu Lys Gly His Leu
325 330 335
Pro Val Leu Ser Glu Gln Ala Glu Ala Asp Lys Glu Thr Leu Ala Ala
340 345 350
Glu Leu Gln Ala Gly Val Thr Ile Arg Ala
355 360
<210> 205
<211> 364
<212> PRT
<213> 柳枝稷()
<220>
<223> 柳枝稷肉桂酰基辅酶A还原酶 (PvCCR)
<400> 205
Met Thr Val Val Asp Ala Val Ser Ala Gly Ala Gly Asp Ala Ala Ala
1 5 10 15
Ala Val Pro Gln Pro Ala Gly Asn Gly Gln Thr Val Cys Val Thr Gly
20 25 30
Ala Ala Gly Tyr Ile Ala Ser Trp Leu Val Lys Leu Leu Leu Glu Lys
35 40 45
Gly Tyr Thr Val Lys Gly Thr Val Arg Asn Pro Asp Asp Pro Lys Asn
50 55 60
Ala His Leu Lys Ala Leu Asp Gly Ala Ala Glu Arg Leu Ile Leu Cys
65 70 75 80
Lys Ala Asp Leu Leu Asp Tyr Asp Ala Ile Cys Arg Ala Val Glu Gly
85 90 95
Cys His Gly Val Phe His Thr Ala Ser Pro Val Thr Asp Asp Pro Glu
100 105 110
Gln Met Val Glu Pro Ala Val Arg Gly Thr Glu Tyr Val Ile Ser Ala
115 120 125
Ala Ala Glu Ala Gly Thr Val Arg Arg Val Val Phe Thr Ser Ser Ile
130 135 140
Gly Ala Val Thr Met Asp Pro Asn Arg Gly Pro Asp Val Val Val Asp
145 150 155 160
Glu Ser Cys Trp Ser Asp Leu Asp Phe Cys Lys Lys Thr Arg Asn Trp
165 170 175
Tyr Cys Tyr Gly Lys Ala Val Ala Glu Gln Ala Ala Trp Glu Ala Ala
180 185 190
Arg Gln Arg Gly Val Asp Leu Val Val Val Asn Pro Val Leu Val Val
195 200 205
Gly Pro Leu Leu Gln Pro Thr Val Asn Ala Ser Ile Ala His Ile Leu
210 215 220
Lys Tyr Leu Asp Gly Ser Ala Arg Thr Phe Ala Asn Ala Val Gln Ala
225 230 235 240
Tyr Val Asp Val Arg Asp Val Ala Ala Ala His Leu Arg Val Phe Glu
245 250 255
Ser Pro Ala Ala Ser Gly Arg His Leu Cys Ala Glu Arg Val Leu His
260 265 270
Arg Glu Asp Val Val Arg Ile Leu Ala Lys Leu Phe Pro Glu Tyr Pro
275 280 285
Val Pro Thr Arg Cys Ser Asp Glu Val Asn Pro Arg Lys Gln Ala Tyr
290 295 300
Lys Phe Thr Asn Gln Lys Leu Arg Asp Leu Gly Leu Glu Phe Arg Pro
305 310 315 320
Val Ser Gln Ser Leu Tyr Asp Thr Val Lys Ser Leu Gln Glu Lys Gly
325 330 335
His Leu Pro Val Leu Ala Asp Glu Gln Ala Pro Glu Ala Ala Pro Gly
340 345 350
Ala Glu Ala Gln Gln Gly Gly Ile Ala Ile Arg Ala
355 360
<210> 206
<211> 374
<212> PRT
<213> 两色高梁()
<220>
<223> 高粱肉桂酰基辅酶A还原酶 (SbCCR)
<400> 206
Met Thr Val Val Asp Ala Val Ser Thr Asp Ala Ala Gly Ala Ala Pro
1 5 10 15
Ala Ala Ala Ala Ala Pro Val Val Val Ala Gln Pro Gly Asn Gly Gln
20 25 30
Thr Val Cys Val Thr Gly Ala Ala Gly Tyr Ile Ala Ser Trp Leu Val
35 40 45
Lys Met Leu Leu Glu Lys Gly Tyr Thr Val Lys Gly Thr Val Arg Asn
50 55 60
Pro Asp Asp Pro Lys Asn Ala His Leu Lys Ala Leu Asp Gly Ala Ala
65 70 75 80
Glu Arg Leu Ile Leu Cys Lys Ala Asp Leu Leu Asp Tyr Asp Ala Ile
85 90 95
Cys Arg Ala Val Gln Gly Cys Gln Gly Val Phe His Thr Ala Ser Pro
100 105 110
Val Thr Asp Asp Pro Glu Gln Met Val Glu Pro Ala Val Arg Gly Thr
115 120 125
Glu Tyr Val Ile Asn Ala Ala Ala Glu Ala Gly Thr Val Arg Arg Val
130 135 140
Val Phe Thr Ser Ser Ile Gly Ala Val Thr Met Asp Pro Ser Arg Gly
145 150 155 160
Pro Asp Val Val Val Asp Glu Ser Cys Trp Ser Asp Leu Glu Phe Cys
165 170 175
Lys Lys Thr Arg Asn Trp Tyr Cys Tyr Gly Lys Ala Val Ala Glu Gln
180 185 190
Ala Ala Trp Asp Ala Ala Arg Gln Arg Gly Val Asp Leu Val Val Val
195 200 205
Asn Pro Val Leu Val Val Gly Pro Leu Leu Gln Pro Thr Val Asn Ala
210 215 220
Ser Ile Ala His Val Leu Lys Tyr Leu Asp Gly Ser Ala Arg Thr Phe
225 230 235 240
Ala Asn Ala Val Gln Ala Tyr Val Asp Val Arg Asp Val Ala Asp Ala
245 250 255
His Leu Arg Val Phe Glu Ser Pro Ala Ala Ser Gly Arg Tyr Leu Cys
260 265 270
Ala Glu Arg Val Leu His Arg Glu Asp Val Val Arg Ile Leu Ala Lys
275 280 285
Leu Phe Pro Glu Tyr Pro Val Pro Thr Arg Cys Ser Asp Glu Val Asn
290 295 300
Pro Arg Lys Gln Pro Tyr Lys Phe Ser Asn Gln Lys Leu Arg Asp Leu
305 310 315 320
Gly Leu Glu Phe Arg Pro Val Ser Gln Ser Leu Tyr Asp Thr Val Lys
325 330 335
Asn Leu Gln Glu Lys Gly His Leu Pro Val Leu Gly Glu Gln Thr Thr
340 345 350
Glu Ala Asp Lys Glu Glu Ala Asn Ala Ala Ala Glu Val Gln Gln Gly
355 360 365
Gly Ile Ala Ile Arg Ala
370
<210> 207
<211> 364
<212> PRT
<213> 柳枝稷()
<220>
<223> 柳枝稷肉桂酰基辅酶A还原酶 (PvCCR)
<400> 207
Met Thr Val Val Asp Ala Val Ser Ala Gly Ala Gly Asp Ala Ala Ala
1 5 10 15
Ala Val Pro Gln Pro Ala Gly Asn Gly Gln Thr Val Cys Val Thr Gly
20 25 30
Ala Ala Gly Tyr Ile Ala Ser Trp Leu Val Lys Leu Leu Leu Glu Lys
35 40 45
Gly Tyr Thr Val Lys Gly Thr Val Arg Asn Pro Asp Asp Pro Lys Asn
50 55 60
Ala His Leu Glu Ala Leu Asp Gly Ala Ala Glu Arg Leu Ile Leu Cys
65 70 75 80
Lys Ala Asp Leu Leu Asp Tyr Asp Ala Ile Cys Arg Ala Val Glu Gly
85 90 95
Cys His Gly Val Phe His Thr Ala Ser Pro Val Thr Asp Asp Pro Glu
100 105 110
Gln Met Val Glu Pro Ala Val Arg Gly Thr Glu Tyr Val Ile Ser Ala
115 120 125
Ala Ala Glu Ala Gly Thr Val Arg Arg Val Val Phe Thr Ser Ser Ile
130 135 140
Gly Ala Val Thr Met Asp Pro Asn Arg Gly Pro Asp Val Val Val Asp
145 150 155 160
Glu Ser Cys Trp Ser Asp Leu Asp Phe Cys Lys Lys Thr Arg Asn Trp
165 170 175
Tyr Cys Tyr Gly Lys Ala Val Ala Glu Gln Ala Ala Trp Glu Ala Ala
180 185 190
Arg Gln Arg Gly Val Asp Leu Val Val Val Asn Pro Val Leu Val Val
195 200 205
Gly Pro Leu Leu Gln Pro Thr Val Asn Ala Ser Ile Ala His Ile Leu
210 215 220
Lys Tyr Leu Asp Gly Ser Ala Arg Thr Phe Ala Asn Ala Val Gln Ala
225 230 235 240
Tyr Val Asp Val Arg Asp Val Ala Ala Ala His Leu Arg Val Phe Glu
245 250 255
Ser Pro Ala Ala Ser Gly Arg His Leu Cys Ala Glu Arg Val Leu His
260 265 270
Arg Glu Asp Val Val Arg Ile Leu Ala Lys Leu Phe Pro Glu Tyr Pro
275 280 285
Val Pro Thr Arg Cys Ser Asp Glu Val Asn Pro Arg Lys Gln Ala Tyr
290 295 300
Lys Phe Thr Asn Gln Lys Leu Arg Asp Leu Gly Leu Glu Phe Arg Pro
305 310 315 320
Val Ser Gln Ser Leu Tyr Asp Thr Val Lys Ser Leu Gln Glu Lys Gly
325 330 335
His Leu Pro Val Leu Ala Asp Glu Gln Ala Pro Glu Ala Ala Pro Gly
340 345 350
Ala Glu Ala Gln Gln Gly Gly Ile Ala Ile Arg Ala
355 360
<210> 208
<211> 372
<212> PRT
<213> 甘蔗()
<220>
<223> 甘蔗肉桂酰基辅酶A还原酶 (SoCCR)
<400> 208
Met Thr Val Val Asp Ala Val Ser Thr Asp Ala Ala Gly Ala Pro Ala
1 5 10 15
Ala Ala Ala Ala Pro Val Gln Gln Pro Gly Asn Gly Gln Thr Val Cys
20 25 30
Val Thr Gly Ala Ala Gly Tyr Ile Ala Ser Trp Leu Val Lys Leu Leu
35 40 45
Leu Glu Lys Gly Tyr Thr Val Lys Gly Thr Val Arg Asn Pro Asp Asp
50 55 60
Pro Lys Asn Ala His Leu Lys Ala Leu Asp Gly Ala Ala Glu Arg Leu
65 70 75 80
Ile Leu Cys Lys Ala Asp Leu Leu Asp Tyr Asp Ala Ile Cys Arg Ala
85 90 95
Val Gln Gly Cys His Gly Val Phe His Thr Ala Ser Pro Val Thr Asp
100 105 110
Asp Pro Glu Gln Met Val Glu Pro Ala Val Arg Gly Thr Glu Tyr Val
115 120 125
Ile Asn Ala Ala Ala Glu Ala Gly Thr Val Arg Arg Val Val Phe Thr
130 135 140
Ser Ser Ile Gly Ala Val Thr Met Asp Pro Ser Arg Gly Pro Asp Val
145 150 155 160
Val Val Asp Glu Ser Cys Trp Ser Asp Leu Glu Phe Cys Lys Lys Thr
165 170 175
Arg Asn Trp Tyr Cys Tyr Gly Lys Ala Val Ala Glu Gln Ala Ala Trp
180 185 190
Asp Ala Ala Arg Gln Arg Gly Val Asp Leu Val Val Val Asn Pro Val
195 200 205
Leu Val Val Gly Pro Leu Leu Gln Pro Thr Val Asn Ala Ser Ile Ala
210 215 220
His Val Val Lys Tyr Leu Asp Gly Ser Ala Arg Thr Phe Ala Asn Ala
225 230 235 240
Val Gln Ala Tyr Val Asp Val Arg Asp Val Ala Asp Ala His Leu Arg
245 250 255
Val Phe Glu Ser Pro Arg Ala Ser Gly Arg Tyr Leu Cys Ala Glu Arg
260 265 270
Val Leu His Arg Glu Asp Val Val Arg Ile Leu Ala Lys Leu Phe Pro
275 280 285
Glu Tyr Pro Val Pro Thr Arg Cys Ser Asp Glu Val Asn Pro Arg Lys
290 295 300
Gln Pro Tyr Lys Phe Ser Asn Gln Lys Leu Arg Asp Leu Gly Leu Glu
305 310 315 320
Phe Arg Pro Val Ser Gln Ser Leu Tyr Asp Thr Val Lys Asn Leu Gln
325 330 335
Glu Lys Gly His Leu Pro Val Leu Gly Glu Gln Thr Thr Glu Ala Asp
340 345 350
Asp Lys Glu Ala Ala Pro Ala Ala Ala Glu Leu Gln Gln Gly Gly Ile
355 360 365
Ala Ile Arg Ala
370
<210> 209
<211> 348
<212> PRT
<213> 大麦()
<220>
<223> 大麦肉桂酰基辅酶A还原酶 (HvCCR)
<400> 209
Met Thr Val Val Asp Ala Ala Ala Ala Val Ala Gln Glu Leu Pro Gly
1 5 10 15
His Gly Gln Thr Val Cys Val Thr Gly Ala Ala Gly Tyr Ile Ala Ser
20 25 30
Trp Leu Val Lys Leu Leu Leu Glu Arg Gly Tyr Thr Val Lys Gly Thr
35 40 45
Val Arg Asn Pro Asp Asp Pro Lys Asn Ala His Leu Lys Ala Leu Asp
50 55 60
Gly Ala Ala Glu Arg Leu Val Leu Cys Lys Ala Asp Leu Leu Asp Tyr
65 70 75 80
Asp Ala Ile Cys Ala Ala Val Glu Gly Cys His Gly Val Phe His Thr
85 90 95
Ala Ser Pro Val Thr Asp Asp Pro Glu Gln Met Val Glu Pro Ala Val
100 105 110
Arg Gly Thr Glu Tyr Val Ile Asp Ala Ala Ala Asp Ala Gly Thr Val
115 120 125
Arg Arg Val Val Phe Thr Ser Ser Ile Gly Ala Val Thr Met Asp Pro
130 135 140
Asn Arg Gly Pro Asp Val Val Val Asp Glu Ser Cys Trp Ser Asp Leu
145 150 155 160
Glu Phe Cys Lys Lys Thr Lys Asn Trp Tyr Cys Tyr Gly Lys Ala Val
165 170 175
Ala Glu Gln Ala Ala Trp Glu Lys Ala Arg Ala Arg Gly Val Asp Leu
180 185 190
Val Val Val Asn Pro Val Leu Val Val Gly Pro Leu Leu Gln Pro Thr
195 200 205
Val Asn Ala Ser Ala Ala His Ile Leu Lys Tyr Leu Asp Gly Ser Ala
210 215 220
Arg Lys Tyr Ala Asn Ala Val Gln Ala Tyr Val Asp Val Arg Asp Val
225 230 235 240
Ala Gly Ala His Leu Arg Val Phe Glu Ala Pro Gln Ala Ser Gly Arg
245 250 255
Tyr Leu Cys Ala Glu Arg Val Leu His Arg Gln Asp Val Val His Ile
260 265 270
Leu Ala Lys Leu Phe Pro Glu Tyr Pro Val Pro Thr Arg Cys Ser Asp
275 280 285
Glu Val Asn Pro Arg Lys Gln Pro Tyr Lys Met Ser Asn Gln Lys Leu
290 295 300
Gln Asp Leu Gly Leu Lys Phe Thr Pro Val Asn Asp Ser Leu Tyr Glu
305 310 315 320
Thr Val Lys Ser Leu Gln Glu Lys Gly His Leu Pro Val Pro Arg Lys
325 330 335
Asp Ile Leu Ala Pro Gln Leu Asp Gly Ala Thr Ala
340 345
<210> 210
<211> 371
<212> PRT
<213> 玉米()
<220>
<223> 玉米肉桂酰基辅酶A还原酶 (ZmCCR)
<400> 210
Met Thr Val Val Asp Ala Val Val Ser Ser Thr Asp Ala Gly Ala Pro
1 5 10 15
Ala Ala Ala Ala Ala Pro Val Pro Ala Gly Asn Gly Gln Thr Val Cys
20 25 30
Val Thr Gly Ala Ala Gly Tyr Ile Ala Ser Trp Leu Val Lys Leu Leu
35 40 45
Leu Glu Lys Gly Tyr Thr Val Lys Gly Thr Val Arg Asn Pro Asp Asp
50 55 60
Pro Lys Asn Ala His Leu Arg Ala Leu Asp Gly Ala Ala Glu Arg Leu
65 70 75 80
Ile Leu Cys Lys Ala Asp Leu Leu Asp Tyr Asp Ala Ile Cys Arg Ala
85 90 95
Val Gln Gly Cys Gln Gly Val Phe His Thr Ala Ser Pro Val Thr Asp
100 105 110
Asp Pro Glu Gln Met Val Glu Pro Ala Val Arg Gly Thr Glu Tyr Val
115 120 125
Ile Asn Ala Ala Ala Glu Ala Gly Thr Val Arg Arg Val Val Phe Thr
130 135 140
Ser Ser Ile Gly Ala Val Thr Met Asp Pro Lys Arg Gly Pro Asp Val
145 150 155 160
Val Val Asp Glu Ser Cys Trp Ser Asp Leu Glu Phe Cys Glu Lys Thr
165 170 175
Arg Asn Trp Tyr Cys Tyr Gly Lys Ala Val Ala Glu Gln Ala Ala Trp
180 185 190
Glu Ala Ala Arg Arg Arg Gly Val Asp Leu Val Val Val Asn Pro Val
195 200 205
Leu Val Val Gly Pro Leu Leu Gln Ala Thr Val Asn Ala Ser Ile Ala
210 215 220
His Ile Leu Lys Tyr Leu Asp Gly Ser Ala Arg Thr Phe Ala Asn Ala
225 230 235 240
Val Gln Ala Tyr Val Asp Val Arg Asp Val Ala Asp Ala His Leu Arg
245 250 255
Val Phe Glu Ser Pro Arg Ala Ser Gly Arg His Leu Cys Ala Glu Arg
260 265 270
Val Leu His Arg Glu Asp Val Val Arg Ile Leu Ala Lys Leu Phe Pro
275 280 285
Glu Tyr Pro Val Pro Ala Arg Cys Ser Asp Glu Val Asn Pro Arg Lys
290 295 300
Gln Pro Tyr Lys Phe Ser Asn Gln Lys Leu Arg Asp Leu Gly Leu Gln
305 310 315 320
Phe Arg Pro Val Ser Gln Ser Leu Tyr Asp Thr Val Lys Asn Leu Gln
325 330 335
Glu Lys Gly His Leu Pro Val Leu Gly Glu Arg Thr Thr Thr Glu Ala
340 345 350
Ala Asp Lys Asp Ala Pro Ala Ala Glu Met Gln Gln Gly Gly Ile Ala
355 360 365
Ile Arg Ala
370
<210> 211
<211> 323
<212> PRT
<213> 江南卷柏()
<220>
<223> 卷柏肉桂酰基辅酶A还原酶 (SmCCR)
<400> 211
Met Thr Ile Glu Ser Val Lys Asp Ala Val Lys Asp Thr Val Cys Val
1 5 10 15
Thr Gly Ala Asn Gly Phe Val Ala Ser Trp Leu Val Lys Leu Leu Leu
20 25 30
Glu Arg Gly Tyr Thr Val His Gly Thr Val Arg Asn Pro Asp Asp Glu
35 40 45
Arg Asn Asp His Leu Lys Ala Phe Asp Gly Ala Lys Glu Arg Leu Ser
50 55 60
Leu Trp Lys Ala Asp Ile Leu Asp Tyr Glu Ser Ile Ser Ala Ala Thr
65 70 75 80
Lys Gly Cys Gln Gly Ile Phe His Thr Ala Cys Pro Val Thr Asp Asp
85 90 95
Leu Gly Ile Val Gln Glu Pro Ala Val Arg Gly Thr Leu Asn Ile Leu
100 105 110
Lys Ala Ala Val Glu His His Val Lys Arg Val Val Leu Thr Ser Ser
115 120 125
Val Gly Ser Val Tyr Met Asp Pro Lys Arg Pro Val Glu Glu Val Val
130 135 140
Ser Glu Glu Met Trp Ser Asp Val Gln Tyr Leu Lys Asp Thr Arg Asn
145 150 155 160
Gly Tyr Cys Leu Ala Lys Thr Leu Ala Glu Ser Ala Ala Trp Glu Phe
165 170 175
Ala Asn Gln Asn His Val Asp Met Val Thr Val Asn Pro Ser Val Val
180 185 190
Leu Gly Pro Leu Leu Gln Ser Thr Met Asn Ala Ser Thr Thr His Ile
195 200 205
Leu Lys Tyr Leu Thr Gly Ala Thr Lys Val Tyr Thr Asn His Cys Gln
210 215 220
Ala Tyr Val Asp Val Arg Asp Val Ala Glu Ala His Ile Leu Val Tyr
225 230 235 240
Glu Glu Pro Ser Ala Cys Gly Arg Tyr Leu Cys Ala Glu Asn Ile Leu
245 250 255
His Arg Gly Glu Val Val Glu Ala Met Ala Lys Leu Phe Pro Asp Tyr
260 265 270
Pro Ile Pro Arg Lys Pro Lys Asp Asp Ser Pro Arg Val Lys Ser Trp
275 280 285
Lys Ile Ser Thr Lys Arg Leu Gln Asp Leu Gly Leu Lys Phe Arg Pro
290 295 300
Phe Glu Glu Tyr Ile Ala Asp Thr Val His Ser Leu Gln Glu Lys Gly
305 310 315 320
Phe Ile Gln
<210> 212
<211> 535
<212> PRT
<213> 拟南芥()
<220>
<223> 木聚糖生物合成酶不规则的木质部8 (IRX8, GAUT12) GI:15239707
<400> 212
Met Gln Leu His Ile Ser Pro Ser Leu Arg His Val Thr Val Val Thr
1 5 10 15
Gly Lys Gly Leu Arg Glu Phe Ile Lys Val Lys Val Gly Ser Arg Arg
20 25 30
Phe Ser Tyr Gln Met Val Phe Tyr Ser Leu Leu Phe Phe Thr Phe Leu
35 40 45
Leu Arg Phe Val Phe Val Leu Ser Thr Val Asp Thr Ile Asp Gly Asp
50 55 60
Pro Ser Pro Cys Ser Ser Leu Ala Cys Leu Gly Lys Arg Leu Lys Pro
65 70 75 80
Lys Leu Leu Gly Arg Arg Val Asp Ser Gly Asn Val Pro Glu Ala Met
85 90 95
Tyr Gln Val Leu Glu Gln Pro Leu Ser Glu Gln Glu Leu Lys Gly Arg
100 105 110
Ser Asp Ile Pro Gln Thr Leu Gln Asp Phe Met Ser Glu Val Lys Arg
115 120 125
Ser Lys Ser Asp Ala Arg Glu Phe Ala Gln Lys Leu Lys Glu Met Val
130 135 140
Thr Leu Met Glu Gln Arg Thr Arg Thr Ala Lys Ile Gln Glu Tyr Leu
145 150 155 160
Tyr Arg His Val Ala Ser Ser Ser Ile Pro Lys Gln Leu His Cys Leu
165 170 175
Ala Leu Lys Leu Ala Asn Glu His Ser Ile Asn Ala Ala Ala Arg Leu
180 185 190
Gln Leu Pro Glu Ala Glu Leu Val Pro Met Leu Val Asp Asn Asn Tyr
195 200 205
Phe His Phe Val Leu Ala Ser Asp Asn Ile Leu Ala Ala Ser Val Val
210 215 220
Ala Lys Ser Leu Val Gln Asn Ala Leu Arg Pro His Lys Ile Val Leu
225 230 235 240
His Ile Ile Thr Asp Arg Lys Thr Tyr Phe Pro Met Gln Ala Trp Phe
245 250 255
Ser Leu His Pro Leu Ser Pro Ala Ile Ile Glu Val Lys Ala Leu His
260 265 270
His Phe Asp Trp Leu Ser Lys Gly Lys Val Pro Val Leu Glu Ala Met
275 280 285
Glu Lys Asp Gln Arg Val Arg Ser Gln Phe Arg Gly Gly Ser Ser Val
290 295 300
Ile Val Ala Asn Asn Lys Glu Asn Pro Val Val Val Ala Ala Lys Leu
305 310 315 320
Gln Ala Leu Ser Pro Lys Tyr Asn Ser Leu Met Asn His Ile Arg Ile
325 330 335
His Leu Pro Glu Leu Phe Pro Ser Leu Asn Lys Val Val Phe Leu Asp
340 345 350
Asp Asp Ile Val Ile Gln Thr Asp Leu Ser Pro Leu Trp Asp Ile Asp
355 360 365
Met Asn Gly Lys Val Asn Gly Ala Val Glu Thr Cys Arg Gly Glu Asp
370 375 380
Lys Phe Val Met Ser Lys Lys Phe Lys Ser Tyr Leu Asn Phe Ser Asn
385 390 395 400
Pro Thr Ile Ala Lys Asn Phe Asn Pro Glu Glu Cys Ala Trp Ala Tyr
405 410 415
Gly Met Asn Val Phe Asp Leu Ala Ala Trp Arg Arg Thr Asn Ile Ser
420 425 430
Ser Thr Tyr Tyr His Trp Leu Asp Glu Asn Leu Lys Ser Asp Leu Ser
435 440 445
Leu Trp Gln Leu Gly Thr Leu Pro Pro Gly Leu Ile Ala Phe His Gly
450 455 460
His Val Gln Thr Ile Asp Pro Phe Trp His Met Leu Gly Leu Gly Tyr
465 470 475 480
Gln Glu Thr Thr Ser Tyr Ala Asp Ala Glu Ser Ala Ala Val Val His
485 490 495
Phe Asn Gly Arg Ala Lys Pro Trp Leu Asp Ile Ala Phe Pro His Leu
500 505 510
Arg Pro Leu Trp Ala Lys Tyr Leu Asp Ser Ser Asp Arg Phe Ile Lys
515 520 525
Ser Cys His Ile Arg Ala Ser
530 535
<210> 213
<211> 533
<212> PRT
<213> 毛果杨()
<220>
<223> 木聚糖生物合成酶不规则的木质部8 (IRX8) 同系物 GI:224126287
<400> 213
Met Gln Leu His Ile Ser Pro Ser Leu Arg His Val Thr Val Phe Pro
1 5 10 15
Gly Lys Gly Val Arg Glu Phe Ile Lys Val Arg Val Gly Ala Arg Arg
20 25 30
Val Ser Tyr Arg Met Leu Phe Tyr Ser Leu Leu Phe Phe Thr Phe Leu
35 40 45
Leu Arg Phe Val Phe Val Leu Ser Thr Val Asp Ser Ile Asp Gly Glu
50 55 60
Thr Lys Cys Ser Thr Leu Gly Cys Leu Gly Lys Arg Leu Gly Pro Arg
65 70 75 80
Ile Leu Gly Arg Arg Leu Asp Ser Ala Val Pro Glu Val Met Phe Gln
85 90 95
Val Leu Glu Gln Pro Leu Gly Asn Asp Glu Leu Lys Gly Arg Ser Asp
100 105 110
Ile Pro Gln Thr Leu Glu Glu Phe Met Asp Glu Val Lys Asn Thr Arg
115 120 125
Leu Asp Ala Lys Thr Phe Ala Leu Lys Leu Arg Glu Met Val Thr Leu
130 135 140
Leu Glu Gln Arg Thr Arg Asn Ala Lys Ile Gln Glu Tyr Leu Tyr Arg
145 150 155 160
His Val Ala Ser Ser Ser Ile Pro Lys Gln Leu His Cys Leu Ala Leu
165 170 175
Arg Leu Ala Ser Glu His Ser Thr Asn Ala Ala Ala Arg Leu Gln Leu
180 185 190
Pro Leu Pro Glu Leu Val Pro Ala Leu Val Asp Asn Thr Tyr Phe His
195 200 205
Phe Val Leu Ala Ser Asp Asn Val Leu Ala Ala Ala Val Val Ala Asn
210 215 220
Ser Leu Val Gln Asn Ala Leu Arg Pro Gln Lys Phe Val Leu His Ile
225 230 235 240
Ile Thr Asp Arg Lys Thr Tyr Ser Pro Met Gln Ala Trp Phe Ser Leu
245 250 255
His Pro Leu Ala Pro Ala Ile Ile Glu Val Lys Ala Leu His His Phe
260 265 270
Asp Trp Phe Ala Lys Gly Lys Val Pro Val Met Glu Ala Met Glu Lys
275 280 285
Asp Gln Arg Val Arg Ser Gln Phe Arg Gly Gly Ser Ser Ala Ile Val
290 295 300
Ala Asn Asn Thr Glu Lys Pro His Ile Ile Ala Ala Lys Leu Gln Thr
305 310 315 320
Leu Ser Pro Lys Tyr Asn Ser Val Met Asn His Ile Arg Ile His Leu
325 330 335
Pro Glu Leu Phe Pro Ser Leu Asn Lys Val Val Phe Leu Asp Asp Asp
340 345 350
Ile Val Val Gln Ser Asp Leu Ser Pro Leu Trp Asp Ile Asp Met Asn
355 360 365
Gly Lys Val Asn Gly Ala Val Glu Thr Cys Arg Gly Glu Asp Lys Phe
370 375 380
Val Met Ser Lys Lys Leu Lys Ser Tyr Leu Asn Phe Ser His Pro Leu
385 390 395 400
Ile Ser Glu Asn Phe Lys Pro Asn Glu Cys Ala Trp Ala Tyr Gly Met
405 410 415
Asn Ile Phe Asp Leu Glu Ala Trp Arg Lys Thr Asn Ile Ser Thr Thr
420 425 430
Tyr His His Trp Val Glu Glu Asn Leu Lys Ser Asp Leu Ser Leu Trp
435 440 445
Gln Leu Gly Thr Leu Pro Pro Gly Leu Ile Ala Phe His Gly His Val
450 455 460
His Val Ile Asp Pro Phe Trp His Met Leu Gly Leu Gly Tyr Gln Glu
465 470 475 480
Asn Thr Ser Leu Ala Asp Ala Glu Thr Ala Gly Val Ile His Phe Asn
485 490 495
Gly Arg Ala Lys Pro Trp Leu Asp Ile Ala Phe Pro Gln Leu Arg Pro
500 505 510
Leu Trp Ala Lys Tyr Ile Asn Phe Ser Asp Lys Phe Ile Lys Gly Cys
515 520 525
His Ile Arg Pro Ser
530
<210> 214
<211> 532
<212> PRT
<213> 毛果杨()
<220>
<223> 木聚糖生物合成酶不规则的木质部8 (IRX8) 同系物 GI:224117396
<400> 214
Met Gln Leu His Ile Ser Pro Ser Leu Arg His Val Thr Val Leu Pro
1 5 10 15
Gly Asn Gly Val Arg Glu Phe Ile Lys Val Lys Val Arg Ala Arg Arg
20 25 30
Val Ser Tyr Arg Met Leu Phe Tyr Ser Leu Leu Phe Phe Thr Phe Leu
35 40 45
Leu Arg Phe Val Phe Leu Leu Ser Thr Ala Asp Thr Ile Asp Ala Glu
50 55 60
Thr Lys Cys Ser Thr Leu Gly Cys Leu Gly Lys Arg Leu Gly Pro Arg
65 70 75 80
Ile Leu Gly Arg Arg Leu Asp Ser Ala Val Pro Glu Val Met Tyr Gln
85 90 95
Val Leu Glu Gln Pro Leu Asp Asn Asp Glu Leu Lys Gly Arg Asp Asp
100 105 110
Ile Pro Gln Thr Leu Glu Glu Phe Met Asp Glu Val Lys Asn Ser Ile
115 120 125
Phe Asp Ala Lys Ala Phe Ala Leu Lys Leu Arg Glu Met Val Thr Leu
130 135 140
Leu Glu Gln Arg Thr Arg Asn Ala Lys Ile Gln Glu Tyr Leu Tyr Arg
145 150 155 160
His Val Ala Ser Ser Ser Ile Pro Lys Gln Leu Leu Cys Leu Ala Leu
165 170 175
Arg Leu Ala His Glu His Ser Thr Asn Ala Ala Ala Arg Arg Gln Leu
180 185 190
Pro Leu Pro Glu Leu Val Pro Ala Leu Val Asp Asn Ser Tyr Phe His
195 200 205
Phe Val Leu Ala Ser Asp Asn Val Leu Ala Ala Ser Val Val Ala Asn
210 215 220
Ser Leu Phe Gln Asn Ala Leu Arg Pro Glu Lys Phe Val Leu His Ile
225 230 235 240
Ile Thr Asp Arg Lys Thr Tyr Ser Pro Met Gln Ala Trp Phe Ser Leu
245 250 255
His Pro Leu Ser Pro Ala Ile Ile Glu Val Lys Ala Leu His His Phe
260 265 270
Asp Trp Phe Ala Lys Gly Lys Val Pro Val Leu Glu Ala Met Glu Lys
275 280 285
Asp Leu Arg Val Arg Ser Arg Phe Arg Gly Gly Ser Ser Ala Ile Val
290 295 300
Glu Ser Asn Thr Asp Lys Pro His Ile Ile Ala Ala Lys Leu Gln Thr
305 310 315 320
Leu Gly Pro Lys Tyr Asn Ser Val Met Asn His Ile Arg Ile His Leu
325 330 335
Pro Glu Leu Phe Pro Ser Leu Asn Lys Val Val Phe Leu Asp Asp Asp
340 345 350
Ile Val Val Gln Thr Asp Leu Ser Pro Leu Trp Asp Ile Asp Met Asn
355 360 365
Gly Lys Val Asn Gly Ala Val Glu Thr Cys Arg Gly Gln Asp Lys Phe
370 375 380
Val Met Ser Lys Arg Leu Lys Asn Tyr Leu Asn Phe Ser His Pro Leu
385 390 395 400
Ile Ala Lys Asn Phe Asn Pro Asn Glu Cys Ala Trp Ala Tyr Gly Met
405 410 415
Asn Ile Phe Asp Leu Glu Ala Trp Arg Lys Thr Asn Ile Ser Ile Thr
420 425 430
Tyr His His Trp Val Glu Glu Asn Leu Lys Ser Gly Leu Ser Leu Trp
435 440 445
Gln Leu Gly Thr Leu Pro Pro Gly Leu Ile Ala Phe His Gly His Val
450 455 460
His Val Ile Asp Pro Phe Trp His Met Leu Gly Leu Gly Tyr Gln Glu
465 470 475 480
Asn Thr Ser Leu Ala Asp Ala Glu Thr Ala Gly Val Ile His Phe Asn
485 490 495
Gly Arg Ala Lys Pro Trp Leu Asp Ile Ala Phe Pro Gln Leu Arg Pro
500 505 510
Leu Trp Ala Lys Tyr Ile Asn Ser Ser Asp Lys Phe Ile Thr Gly Cys
515 520 525
His Ile Arg Thr
530
<210> 215
<211> 528
<212> PRT
<213> 毛果杨()
<220>
<223> 木聚糖生物合成酶不规则的木质部8 (IRX8) 同系物 GI:224141469
<400> 215
Met Arg Ser Ile Thr Ile Ser Ser Ser Ser Asn Asn Gly Phe Ile Asp
1 5 10 15
Leu Met Lys Ile Lys Val Ala Ala Arg His Ile Ser Tyr Arg Thr Leu
20 25 30
Phe His Thr Ile Leu Ile Leu Ala Phe Leu Leu Pro Phe Val Phe Ile
35 40 45
Leu Thr Ala Leu Val Thr Leu Glu Gly Val Asn Lys Cys Ser Ser Phe
50 55 60
Asp Cys Leu Gly Arg Arg Leu Gly Pro Arg Leu Leu Gly Arg Val Asp
65 70 75 80
Asp Ser Gly Arg Leu Val Lys Asp Phe Tyr Lys Ile Leu Asn Gln Val
85 90 95
Lys Asn Glu Glu Ile Pro Asp Gly Val Lys Leu Pro Ala Ser Phe Ser
100 105 110
His Leu Val Ser Glu Met Lys Asn Asn Gln Tyr Asp Ala Arg Thr Phe
115 120 125
Ala Phe Met Leu Arg Ala Met Met Glu Lys Leu Glu Arg Glu Ile Arg
130 135 140
Glu Ser Lys Phe Ser Glu Leu Met Asn Lys His Phe Ala Ala Ser Ser
145 150 155 160
Ile Pro Lys Ser Ile His Cys Leu Ser Leu Arg Leu Thr Asp Glu Tyr
165 170 175
Ser Ser Asn Ala His Ala Arg Lys Gln Leu Pro Ser Pro Glu Phe Leu
180 185 190
Pro Leu Leu Ser Asp Asn Ser Tyr His His Phe Val Leu Ser Thr Asp
195 200 205
Asn Ile Leu Ala Ala Ser Val Val Val Thr Ser Thr Ile Gln Ser Ser
210 215 220
Leu Lys Pro Asp Asn Ile Val Phe His Ile Ile Thr Asp Lys Lys Thr
225 230 235 240
Tyr Ala Gly Met His Ser Trp Phe Ala Leu Asn Pro Val Ser Pro Ala
245 250 255
Ile Val Glu Val Lys Gly Val His Gln Phe Asp Trp Leu Thr Arg Glu
260 265 270
Asn Val Pro Val Leu Glu Ala Val Glu Asn His Asn Gly Ile Arg Asn
275 280 285
Tyr Tyr His Gly Asn His Ile Ala Gly Ala Asn Leu Ser Asp Thr Thr
290 295 300
Pro Arg Arg Phe Ala Ser Lys Leu Gln Ala Arg Ser Pro Lys Tyr Ile
305 310 315 320
Ser Ile Leu Asn His Leu Arg Ile Tyr Ile Pro Glu Leu Phe Pro Ser
325 330 335
Leu Asp Lys Val Val Phe Leu Asp Asp Asp Val Val Ile Gln Arg Asp
340 345 350
Leu Ser Pro Leu Trp Glu Ile Asp Leu Lys Gly Lys Val Asn Gly Ala
355 360 365
Val Glu Thr Cys Lys Gly Glu Asp Glu Trp Val Met Ser Lys His Phe
370 375 380
Lys Asn Tyr Phe Asn Phe Ser His Pro Leu Ile Ala Lys Asn Leu Asp
385 390 395 400
Pro Asp Glu Cys Ala Trp Ala Tyr Gly Met Asn Ile Phe Asp Leu Arg
405 410 415
Ala Trp Arg Lys Thr Asn Ile Arg Glu Thr Tyr His Ser Trp Leu Lys
420 425 430
Glu Asn Leu Lys Ser Asn Leu Thr Met Trp Lys Leu Gly Thr Leu Pro
435 440 445
Pro Ala Leu Ile Ala Phe Lys Gly His Val His Pro Ile Asp Pro Ser
450 455 460
Trp His Met Leu Gly Leu Gly Tyr Gln Asn Lys Thr Asn Ile Glu Ser
465 470 475 480
Val Lys Lys Ala Ala Val Ile His Tyr Asn Gly Gln Ala Lys Pro Trp
485 490 495
Leu Glu Ile Gly Phe Glu His Leu Arg Pro Phe Trp Thr Lys Tyr Val
500 505 510
Asn Tyr Ser Asn Asp Phe Ile Arg Asn Cys His Ile Leu Asp Ser Val
515 520 525
<210> 216
<211> 528
<212> PRT
<213> 毛果杨()
<220>
<223> 木聚糖生物合成酶不规则的木质部8 (IRX8) 同系物 GI:224077712
<400> 216
Met Arg Ser Ile Thr Ile Ser Ser Ser Gly Asn Asn Gly Phe Ile Asp
1 5 10 15
Ser Met Lys Ile Lys Val Ala Ala Arg His Ile Ser Tyr Arg Thr Leu
20 25 30
Phe His Thr Ile Leu Ile Leu Ala Phe Leu Leu Pro Phe Val Phe Ile
35 40 45
Leu Thr Ala Leu Val Thr Leu Glu Gly Val Asn Lys Cys Ser Ser Phe
50 55 60
Asp Cys Leu Gly Arg Arg Leu Gly Pro Arg Leu Leu Gly Arg Val Asp
65 70 75 80
Asp Ser Gly Arg Leu Val Lys Asp Phe Tyr Lys Ile Leu Asn Gln Val
85 90 95
Lys Asn Glu Glu Ile Pro Asp Gly Val Lys Leu Pro Ala Ser Phe Asn
100 105 110
His Leu Val Ser Glu Met Lys Asn Asn Gln Tyr Asp Ala Arg Thr Phe
115 120 125
Ala Phe Met Leu Arg Ala Met Met Glu Lys Leu Glu Arg Glu Ile Arg
130 135 140
Glu Ser Lys Phe Ala Glu Leu Met Asn Lys His Phe Ala Ala Ser Ser
145 150 155 160
Ile Pro Lys Ser Ile His Cys Leu Ser Leu Arg Leu Thr Asp Glu Tyr
165 170 175
Ser Ser Asn Ala His Ala Arg Thr Gln Leu Pro Ser Pro Glu Phe Leu
180 185 190
Pro Leu Leu Ser Asp Asn Ser Tyr His His Phe Val Leu Ser Thr Asp
195 200 205
Asn Ile Leu Ala Ala Ser Val Val Val Thr Ser Thr Val Gln Ser Ser
210 215 220
Leu Lys Pro Asp Arg Ile Val Phe His Ile Ile Thr Asp Lys Lys Thr
225 230 235 240
Tyr Ala Gly Met His Ser Trp Phe Ala Leu Asn Pro Ala Ser Pro Ala
245 250 255
Ile Val Glu Val Lys Gly Val His Gln Phe Asp Trp Leu Thr Arg Glu
260 265 270
Asn Val Pro Val Leu Glu Ala Val Glu Asn His Asn Gly Ile Arg Asp
275 280 285
Tyr Tyr His Gly Asn His Ile Ala Gly Ala Asn Leu Ser Asp Thr Thr
290 295 300
Pro Arg Arg Phe Ala Ser Lys Leu Gln Ala Arg Ser Pro Lys Tyr Ile
305 310 315 320
Ser Leu Leu Asn His Leu Arg Ile Tyr Ile Pro Glu Leu Phe Pro Asn
325 330 335
Leu Asp Lys Val Val Phe Leu Asp Asp Asp Val Val Ile Gln His Asp
340 345 350
Leu Ser Pro Leu Trp Glu Ile Asp Leu Gln Gly Lys Val Asn Gly Ala
355 360 365
Val Glu Thr Cys Lys Gly Glu Asp Glu Trp Val Met Ser Lys His Leu
370 375 380
Lys Asn Tyr Phe Asn Phe Ser His Pro Leu Ile Ala Lys Asn Leu Asp
385 390 395 400
Pro Asp Glu Cys Ala Trp Ala Tyr Gly Met Asn Ile Phe Asp Leu His
405 410 415
Ala Trp Arg Asn Thr Asn Ile Arg Glu Thr Tyr His Ser Trp Met Lys
420 425 430
Glu Asn Leu Lys Ser Asn Leu Thr Met Trp Lys Leu Gly Thr Leu Pro
435 440 445
Pro Ser Leu Ile Ala Phe Lys Gly His Val His Pro Ile Asp Pro Phe
450 455 460
Trp His Met Leu Gly Leu Gly Tyr Gln Asn Asn Thr Asn Ile Glu Ser
465 470 475 480
Val Lys Lys Ala Ala Val Ile His Tyr Asn Gly Gln Ser Lys Pro Trp
485 490 495
Leu Glu Ile Gly Phe Glu His Leu Arg Pro Phe Trp Thr Lys Tyr Val
500 505 510
Asn Tyr Ser Asn Asp Phe Ile Arg Asn Cys His Ile Leu Asp Ser Val
515 520 525
<210> 217
<211> 531
<212> PRT
<213> 江南卷柏()
<220>
<223> 木聚糖生物合成酶不规则的木质部8 (IRX8) 同系物 GI:302803855
<400> 217
Met Gln Val His Ile Ser Pro Ser Met Arg Arg Ile Thr Ile Ser Thr
1 5 10 15
Ser Asn Gly Phe Leu Asp Pro Leu Lys Ile Lys Val Ala Ala Arg Tyr
20 25 30
Leu Ser His Arg Cys Leu Phe Trp Ser Ile Leu Phe Leu Ala Phe Leu
35 40 45
Leu Pro Phe Ile Phe Ile Thr Thr Ala Leu Ile Thr Leu Glu Glu Val
50 55 60
Asn Lys Cys Ser Ser Met Tyr Cys Leu Gly Arg Lys Ile Gly Pro Lys
65 70 75 80
Leu His Trp Arg Ser Asp Pro Thr Arg Gln Ser Arg His Val His Ser
85 90 95
Ile Val Met Gln Ala Ser Lys Ser Asp Leu Pro Ala Gly Glu Asn Val
100 105 110
Pro Glu Ser Phe Ser Glu Phe Val Ala Glu Val Glu Ala Asn Arg Tyr
115 120 125
Asp Gly Lys Thr Cys Ile Leu Lys Leu Lys Ala Met Leu Glu Leu Gln
130 135 140
Glu Gln Arg Thr Arg Thr Ala Lys Leu Gln Glu Ala Val Tyr Arg His
145 150 155 160
Phe Ala Ser Ser Gly Ile Pro Lys Ser Met His Cys Leu Ala Leu Lys
165 170 175
Leu Thr Ala Glu Tyr Ser Ser Asn Ala Asn Ala Arg Arg Glu Leu Pro
180 185 190
Ser Pro Glu Leu Thr Tyr Arg Leu Thr Asp His Ser Phe His His Phe
195 200 205
Val Leu Ala Thr Asp Asn Val Leu Ala Ala Ser Val Val Val Ser Ser
210 215 220
Val Ile Arg Asn Ser Ala Gln Pro Gln Lys Val Val Phe His Val Val
225 230 235 240
Thr Asp Lys Lys Thr Tyr Ala Ala Met His Ala Trp Phe Ala Leu Asn
245 250 255
Pro Leu Pro Pro Ala Ile Val Glu Val Lys Ser Leu His Gln Phe Glu
260 265 270
Trp Leu Thr Lys Asp Asn Ile Pro Val Leu Glu Ala Met Glu Asn His
275 280 285
Ser Asp Ile Arg Arg Tyr Tyr His Gly Asp His Thr Ala Gly Ala Asp
290 295 300
Leu Asn Val Ser Pro Thr Ile Leu Ala Ser Arg Leu Gln Ala Arg Ser
305 310 315 320
Pro Lys Tyr Ile Ser Ile Leu Asn His Leu Arg Ile Tyr Leu Pro Glu
325 330 335
Leu Phe Pro Glu Leu Asp Lys Val Val Phe Leu Asp Asp Asp Val Val
340 345 350
Ala Gln Lys Asp Leu Ser Pro Leu Phe Gly Ile Asp Leu Asn Gly Arg
355 360 365
Val Asn Gly Ala Val Glu Thr Cys Arg Gly Glu Asp Pro Tyr Val Met
370 375 380
Ser Lys Arg Phe Lys Thr Tyr Phe Asn Phe Ser His Pro Leu Ile Ala
385 390 395 400
Asn His Phe Asp Pro Glu Lys Cys Ala Trp Ala Tyr Gly Met Asn Val
405 410 415
Phe Asp Leu Gln Ala Trp Arg Arg Thr Asp Ile Thr Lys Thr Tyr His
420 425 430
Tyr Trp Gln Lys Gln Asn Leu Asn Ser Asn Leu Thr Leu Trp Arg Leu
435 440 445
Gly Thr Leu Pro Pro Ala Leu Ile Ala Phe Asp Gly Tyr Val Tyr Pro
450 455 460
Ile Asp Ser Gln Trp His Met Leu Gly Leu Gly Tyr His Val Lys Ser
465 470 475 480
Asn Leu Asp Ser Val Gln Lys Ala Ala Val Ile His Tyr Asn Gly Gln
485 490 495
Ala Lys Pro Trp Leu Asp Ile Gly Phe Ser Val Leu Arg Pro Phe Trp
500 505 510
Thr Lys Tyr Val Asn Tyr Ser Asn Glu Phe Ile Arg Arg Cys Asn Ile
515 520 525
Leu Glu Thr
530
<210> 218
<211> 533
<212> PRT
<213> 拟南芥()
<220>
<223> 木聚糖生物合成酶不规则的木质部8 (IRX8) GI:30678270
<400> 218
Met Gln Leu His Ile Ser Pro Ser Met Arg Ser Ile Thr Ile Ser Ser
1 5 10 15
Ser Asn Glu Phe Ile Asp Leu Met Lys Ile Lys Val Ala Ala Arg His
20 25 30
Ile Ser Tyr Arg Thr Leu Phe His Thr Ile Leu Ile Leu Ala Phe Leu
35 40 45
Leu Pro Phe Val Phe Ile Leu Thr Ala Val Val Thr Leu Glu Gly Val
50 55 60
Asn Lys Cys Ser Ser Phe Asp Cys Phe Gly Arg Arg Leu Gly Pro Arg
65 70 75 80
Leu Leu Gly Arg Ile Asp Asp Ser Glu Gln Arg Leu Val Arg Asp Phe
85 90 95
Tyr Lys Ile Leu Asn Glu Val Ser Thr Gln Glu Ile Pro Asp Gly Leu
100 105 110
Lys Leu Pro Glu Ser Phe Ser Gln Leu Val Ser Asp Met Lys Asn Asn
115 120 125
His Tyr Asp Ala Lys Thr Phe Ala Leu Val Phe Arg Ala Met Val Glu
130 135 140
Lys Phe Glu Arg Asp Leu Arg Glu Ser Lys Phe Ala Glu Leu Met Asn
145 150 155 160
Lys His Phe Ala Ala Ser Ser Ile Pro Lys Gly Ile His Cys Leu Ser
165 170 175
Leu Arg Leu Thr Asp Glu Tyr Ser Ser Asn Ala His Ala Arg Arg Gln
180 185 190
Leu Pro Ser Pro Glu Leu Leu Pro Val Leu Ser Asp Asn Ala Tyr His
195 200 205
His Phe Val Leu Ala Thr Asp Asn Ile Leu Ala Ala Ser Val Val Val
210 215 220
Ser Ser Ala Val Gln Ser Ser Ser Lys Pro Glu Lys Ile Val Phe His
225 230 235 240
Val Ile Thr Asp Lys Lys Thr Tyr Ala Gly Met His Ser Trp Phe Ala
245 250 255
Leu Asn Ser Val Ala Pro Ala Ile Val Glu Val Lys Ser Val His Gln
260 265 270
Phe Asp Trp Leu Thr Arg Glu Asn Val Pro Val Leu Glu Ala Val Glu
275 280 285
Ser His Asn Ser Ile Arg Asn Tyr Tyr His Gly Asn His Ile Ala Gly
290 295 300
Ala Asn Leu Ser Glu Thr Thr Pro Arg Thr Phe Ala Ser Lys Leu Gln
305 310 315 320
Ser Arg Ser Pro Lys Tyr Ile Ser Leu Leu Asn His Leu Arg Ile Tyr
325 330 335
Leu Pro Glu Leu Phe Pro Asn Leu Asp Lys Val Val Phe Leu Asp Asp
340 345 350
Asp Ile Val Ile Gln Lys Asp Leu Ser Pro Leu Trp Asp Ile Asp Leu
355 360 365
Asn Gly Lys Val Asn Gly Ala Val Glu Thr Cys Arg Gly Glu Asp Val
370 375 380
Trp Val Met Ser Lys Arg Leu Arg Asn Tyr Phe Asn Phe Ser His Pro
385 390 395 400
Leu Ile Ala Lys His Leu Asp Pro Glu Glu Cys Ala Trp Ala Tyr Gly
405 410 415
Met Asn Ile Phe Asp Leu Arg Thr Trp Arg Lys Thr Asn Ile Arg Glu
420 425 430
Thr Tyr His Ser Trp Leu Lys Glu Asn Leu Lys Ser Asn Leu Thr Met
435 440 445
Trp Lys Leu Gly Thr Leu Pro Pro Ala Leu Ile Ala Phe Lys Gly His
450 455 460
Val Gln Pro Ile Asp Ser Ser Trp His Met Leu Gly Leu Gly Tyr Gln
465 470 475 480
Ser Lys Thr Asn Leu Glu Asn Ala Lys Lys Ala Ala Val Ile His Tyr
485 490 495
Asn Gly Gln Ser Lys Pro Trp Leu Glu Ile Gly Phe Glu His Leu Arg
500 505 510
Pro Phe Trp Thr Lys Tyr Val Asn Tyr Ser Asn Asp Phe Ile Lys Asn
515 520 525
Cys His Ile Leu Glu
530
<210> 219
<211> 532
<212> PRT
<213> 拟南芥()
<220>
<223> 木聚糖生物合成酶不规则的木质部8 (IRX8) GI:30685369
<400> 219
Met Gln Leu His Ile Ser Pro Ser Met Arg Ser Ile Thr Ile Ser Ser
1 5 10 15
Ser Asn Glu Phe Ile Asp Leu Met Lys Ile Lys Val Ala Ala Arg His
20 25 30
Ile Ser Tyr Arg Thr Leu Phe His Thr Ile Leu Ile Leu Ala Phe Leu
35 40 45
Leu Pro Phe Val Phe Ile Leu Thr Ala Val Val Thr Leu Glu Gly Val
50 55 60
Asn Lys Cys Ser Ser Ile Asp Cys Leu Gly Arg Arg Ile Gly Pro Arg
65 70 75 80
Leu Leu Gly Arg Val Asp Asp Ser Glu Arg Leu Ala Arg Asp Phe Tyr
85 90 95
Lys Ile Leu Asn Glu Val Ser Thr Gln Glu Ile Pro Asp Gly Leu Lys
100 105 110
Leu Pro Asn Ser Phe Ser Gln Leu Val Ser Asp Met Lys Asn Asn His
115 120 125
Tyr Asp Ala Lys Thr Phe Ala Leu Val Leu Arg Ala Met Met Glu Lys
130 135 140
Phe Glu Arg Asp Met Arg Glu Ser Lys Phe Ala Glu Leu Met Asn Lys
145 150 155 160
His Phe Ala Ala Ser Ser Ile Pro Lys Gly Ile His Cys Leu Ser Leu
165 170 175
Arg Leu Thr Asp Glu Tyr Ser Ser Asn Ala His Ala Arg Arg Gln Leu
180 185 190
Pro Ser Pro Glu Phe Leu Pro Val Leu Ser Asp Asn Ala Tyr His His
195 200 205
Phe Ile Leu Ser Thr Asp Asn Ile Leu Ala Ala Ser Val Val Val Ser
210 215 220
Ser Ala Val Gln Ser Ser Ser Lys Pro Glu Lys Ile Val Phe His Ile
225 230 235 240
Ile Thr Asp Lys Lys Thr Tyr Ala Gly Met His Ser Trp Phe Ala Leu
245 250 255
Asn Ser Val Ala Pro Ala Ile Val Glu Val Lys Gly Val His Gln Phe
260 265 270
Asp Trp Leu Thr Arg Glu Asn Val Pro Val Leu Glu Ala Val Glu Ser
275 280 285
His Asn Gly Val Arg Asp Tyr Tyr His Gly Asn His Val Ala Gly Ala
290 295 300
Asn Leu Thr Glu Thr Thr Pro Arg Thr Phe Ala Ser Lys Leu Gln Ser
305 310 315 320
Arg Ser Pro Lys Tyr Ile Ser Leu Leu Asn His Leu Arg Ile Tyr Ile
325 330 335
Pro Glu Leu Phe Pro Asn Leu Asp Lys Val Val Phe Leu Asp Asp Asp
340 345 350
Ile Val Val Gln Gly Asp Leu Thr Pro Leu Trp Asp Val Asp Leu Gly
355 360 365
Gly Lys Val Asn Gly Ala Val Glu Thr Cys Arg Gly Glu Asp Glu Trp
370 375 380
Val Met Ser Lys Arg Leu Arg Asn Tyr Phe Asn Phe Ser His Pro Leu
385 390 395 400
Ile Ala Lys His Leu Asp Pro Glu Glu Cys Ala Trp Ala Tyr Gly Met
405 410 415
Asn Ile Phe Asp Leu Gln Ala Trp Arg Lys Thr Asn Ile Arg Glu Thr
420 425 430
Tyr His Ser Trp Leu Arg Glu Asn Leu Lys Ser Asn Leu Thr Met Trp
435 440 445
Lys Leu Gly Thr Leu Pro Pro Ala Leu Ile Ala Phe Lys Gly His Val
450 455 460
His Ile Ile Asp Ser Ser Trp His Met Leu Gly Leu Gly Tyr Gln Ser
465 470 475 480
Lys Thr Asn Ile Glu Asn Val Lys Lys Ala Ala Val Ile His Tyr Asn
485 490 495
Gly Gln Ser Lys Pro Trp Leu Glu Ile Gly Phe Glu His Leu Arg Pro
500 505 510
Phe Trp Thr Lys Tyr Val Asn Tyr Ser Asn Asp Phe Ile Lys Asn Cys
515 520 525
His Ile Leu Glu
530
<210> 220
<211> 554
<212> PRT
<213> 水稻()
<220>
<223> 木聚糖生物合成酶不规则的木质部8 (IRX8) 同系物 GI:115489272
<400> 220
Met Gln Ile Arg Leu Ser Pro Ser Met Arg Ser Ile Thr Ile Ser Ser
1 5 10 15
Ser Gly Ile Gly Ile Gly Gly Gly Asn Gly Gly Gly Gly Gly Gly Gly
20 25 30
Gly Gly Val Gly Ser Gly Leu Leu Asp Leu Met Lys Leu Lys Ala Ala
35 40 45
Ala Arg His Phe Ser Tyr Arg Thr Val Phe His Thr Val Leu Ile Leu
50 55 60
Ala Phe Leu Leu Pro Phe Val Phe Ile Leu Thr Ala Leu Val Thr Leu
65 70 75 80
Glu Gly Phe Asn Lys Cys Ser Ser Leu Asp Cys Leu Gly Arg Arg Leu
85 90 95
Gly Pro Arg Leu Leu Gly Arg Gly Asn Asp Gly Ser Met Arg Val Met
100 105 110
Arg Asp Leu Tyr Arg Met Leu Asp Glu Ile Asn Ser Glu Glu Val Pro
115 120 125
Val Asp Leu Lys Val Pro Asp Ser Phe Asp Glu Phe Ile Trp Asp Met
130 135 140
Lys Asn Asn Asp Tyr Asp Leu Arg Ser Phe Ala Phe Arg Leu Lys Ala
145 150 155 160
Thr Met Glu Ser Met Asp Arg Glu Leu Arg Thr Ser Arg Leu Ser Glu
165 170 175
Gln Leu Asn Lys His Tyr Ala Ala Ile Ala Ile Pro Lys Gly Leu His
180 185 190
Cys Leu Ser Leu Arg Leu Thr Asp Glu Tyr Ser Ser Asn Ala Leu Ala
195 200 205
Arg Lys Gln Leu Pro Pro Pro Glu Leu Ile Pro Arg Leu Ser Asp Asn
210 215 220
Ser Tyr Leu His Phe Val Leu Ala Ser Asp Asn Ile Leu Ala Ala Ser
225 230 235 240
Val Val Val Ser Ser Thr Ile Arg Ser Ser Leu Lys Pro Gly Arg Ile
245 250 255
Val Phe His Val Ile Thr Asp Lys Lys Thr Tyr Pro Ala Met His Ser
260 265 270
Trp Phe Ala Leu Asn Thr Leu Ser Pro Ala Ile Val Glu Val Lys Gly
275 280 285
Val His Gln Phe Asp Trp Leu Thr Arg Glu Asn Val Pro Val Leu Glu
290 295 300
Ala Ile Glu Thr Gln His Thr Val Arg Ser Arg Phe His Gly Asn His
305 310 315 320
Leu Ala Arg Asn Ser Ala Gly Asp Ser Pro Arg Val Phe Ala Ala Lys
325 330 335
Leu Gln Ala Gly Ser Pro Thr Tyr Thr Ser Val Leu Asn His Ile Arg
340 345 350
Ile Tyr Leu Pro Glu Leu Phe Pro Asn Leu Asn Lys Val Val Phe Leu
355 360 365
Asp Asp Asp Val Val Val Gln Arg Asp Leu Ser Ser Leu Trp Asp Ile
370 375 380
Asp Leu Gly Gly Lys Val Asn Gly Ala Val Glu Thr Cys Arg Gly Gly
385 390 395 400
Asp Thr Trp Val Met Ser Lys Arg Phe Arg Asn Tyr Phe Asn Phe Ser
405 410 415
His Pro Leu Ile Ala Asn Asn Phe Asp Pro Ser Glu Cys Ala Trp Ala
420 425 430
Tyr Gly Met Asn Ile Phe Asp Leu Ser Ala Trp Arg Lys Thr Ser Ile
435 440 445
Lys Asp Lys Tyr His His Trp Val Arg Glu Asn Leu Ser Ser Asn Phe
450 455 460
Thr Leu Trp Arg Leu Gly Thr Leu Pro Pro Gly Leu Ile Ala Phe Arg
465 470 475 480
Gly His Val His Pro Ile Asp Pro Ser Trp His Leu Leu Gly Leu Gly
485 490 495
Tyr Gln Glu Lys Thr Asp Ile Pro Ser Val Gln Lys Ala Ala Val Ile
500 505 510
His Tyr Asn Gly Gln Ser Lys Pro Trp Leu Asp Ile Gly Phe Lys His
515 520 525
Leu Gln Pro Phe Trp Thr Arg His Val Asn Tyr Ser Asn Glu Phe Ile
530 535 540
Arg Asn Cys His Ile Met Glu Pro Gln Leu
545 550
<210> 221
<211> 531
<212> PRT
<213> 毛果杨()
<220>
<223> 木聚糖生物合成酶不规则的木质部8 (IRX8) 同系物 GI:224131384
<400> 221
Met Lys Phe Tyr Ile Ser Thr Thr Gly Ile Lys Arg Val Thr Ile Ser
1 5 10 15
Thr Thr Asn Ser Ser Ala Lys Gly Ser Thr Val Ala Thr Arg Arg Ile
20 25 30
Thr Arg Arg Thr Phe Leu Pro Val Val Leu Leu Leu Ser Ile Val Leu
35 40 45
Pro Phe Leu Phe Val Arg Ile Ala Phe Leu Val Leu Glu Ser Ala Ser
50 55 60
Ala Cys Asn Ser Ala Leu Asp Cys Ile Gly Trp Gly Leu Leu Gly Gly
65 70 75 80
Ser Glu Ala Ser Leu Leu Arg Glu Glu Leu Thr Arg Ala Leu Met Glu
85 90 95
Ala Lys Glu Gly Arg Gly Thr Asn Asp Gly Asp Tyr Arg Thr Glu Gly
100 105 110
Ser Thr Glu Ser Phe Asn Val Leu Val Asn Glu Met Thr Ser Asn Gln
115 120 125
Gln Asp Ile Lys Thr Phe Ala Phe Arg Thr Lys Ala Met Leu Ser Met
130 135 140
Met Glu Leu Lys Val Gln Ser Ala Arg Glu Gln Glu Ser Ile Asn Trp
145 150 155 160
His Leu Ala Ser His Gly Val Pro Lys Ser Leu His Cys Leu Cys Leu
165 170 175
Lys Leu Ala Glu Glu Tyr Ala Val Asn Ala Met Ala Arg Ser His Leu
180 185 190
Pro Pro Pro Glu Tyr Val Ser Arg Leu Thr Asp Pro Ser Phe His His
195 200 205
Val Val Leu Leu Thr Asp Asn Val Leu Ala Ala Ser Val Val Ile Ser
210 215 220
Ser Thr Val Gln His Ser Ala Asn Pro Glu Lys Leu Val Phe His Ile
225 230 235 240
Val Thr Asp Lys Lys Thr Tyr Ile Pro Met Asn Ala Trp Phe Ala Ile
245 250 255
Asn Pro Ile Lys Ser Ala Ala Val Glu Val Lys Gly Leu His Gln Tyr
260 265 270
Asp Trp Ser His Glu Val Asn Val His Val Lys Glu Met Leu Glu Ile
275 280 285
His Arg Leu Ile Trp Ser His Tyr Asn Asp Asn Leu Arg Asn Ala Asn
290 295 300
Phe Gln His Glu Gly Val Asn Arg Arg Ser Leu Glu Ala Leu Thr Pro
305 310 315 320
Ser Cys Leu Ser Leu Leu Asn His Leu Arg Ile Tyr Ile Pro Glu Leu
325 330 335
Phe Pro Asp Leu Asn Lys Ile Val Phe Leu Asp Glu Asp Val Val Val
340 345 350
Gln His Asp Met Ser Ser Leu Trp Glu Leu Asp Leu Asn Lys Lys Val
355 360 365
Val Gly Ala Val Val Asp Ser Trp Cys Gly Asp Asn Cys Cys Pro Gly
370 375 380
Lys Lys Tyr Lys Asp Tyr Leu Asn Phe Ser Tyr Pro Ile Ile Ser Ser
385 390 395 400
Asn Phe Asp His Asp Arg Cys Val Trp Leu Tyr Gly Val Asn Val Phe
405 410 415
Asp Leu Glu Ala Trp Arg Arg Val Lys Ile Thr Thr Asn Tyr His Lys
420 425 430
Trp Leu Lys His Asn Leu Asn Phe Gly Met Glu Leu Trp Gln Pro Gly
435 440 445
Val His Pro Pro Ala Leu Leu Ala Phe Glu Gly Gln Val His Pro Ile
450 455 460
Asp Pro Ser Trp His Val Gly Gly Leu Gly Tyr Arg Pro Pro Gln Ala
465 470 475 480
His Asn Ile Lys Met Leu Gly Asp Ala Ala Val Leu His Phe Ser Gly
485 490 495
Pro Ala Lys Pro Trp Leu Asp Ile Gly Phe Pro Glu Leu Arg Ser Leu
500 505 510
Trp Asn Arg His Val Asn Phe Ser Asp Lys Phe Ile Arg Lys Cys Arg
515 520 525
Ile Leu Gly
530
<210> 222
<211> 540
<212> PRT
<213> 拟南芥()
<220>
<223> 木聚糖生物合成酶不规则的木质部8 (IRX8) GI:22331857
<400> 222
Met Lys Phe Tyr Ile Ser Ala Thr Gly Ile Lys Lys Val Thr Ile Ser
1 5 10 15
Asn Pro Gly Val Gly Ile Gly Lys Gly Ser Gly Gly Cys Ala Ala Ala
20 25 30
Ala Ala Ala Leu Ala Ala Arg Arg Phe Ser Ser Arg Thr Leu Leu Leu
35 40 45
Leu Leu Leu Leu Leu Ala Ile Val Leu Pro Phe Ile Phe Val Arg Phe
50 55 60
Ala Phe Leu Val Leu Glu Ser Ala Ser Val Cys Asp Ser Pro Leu Asp
65 70 75 80
Cys Met Gly Leu Arg Leu Phe Arg Gly Gly Asp Thr Ser Leu Lys Ile
85 90 95
Gly Glu Glu Leu Thr Arg Ala Leu Val Glu Glu Thr Thr Asp His Gln
100 105 110
Asp Val Asn Gly Arg Gly Thr Lys Gly Ser Leu Glu Ser Phe Asp Asp
115 120 125
Leu Val Lys Glu Met Thr Leu Lys Arg Arg Asp Ile Arg Ala Phe Ala
130 135 140
Ser Val Thr Lys Lys Met Leu Leu Gln Met Glu Arg Lys Val Gln Ser
145 150 155 160
Ala Lys His His Glu Leu Val Tyr Trp His Leu Ala Ser His Gly Ile
165 170 175
Pro Lys Ser Leu His Cys Leu Ser Leu Arg Leu Thr Glu Glu Tyr Ser
180 185 190
Val Asn Ala Met Ala Arg Met Arg Leu Pro Pro Pro Glu Ser Val Ser
195 200 205
Arg Leu Thr Asp Pro Ser Phe His His Ile Val Leu Leu Thr Asp Asn
210 215 220
Val Leu Ala Ala Ser Val Val Ile Ser Ser Thr Val Gln Asn Ala Val
225 230 235 240
Asn Pro Glu Lys Phe Val Phe His Ile Val Thr Asp Lys Lys Thr Tyr
245 250 255
Thr Pro Met His Ala Trp Phe Ala Ile Asn Ser Ala Ser Ser Pro Val
260 265 270
Val Glu Val Lys Gly Leu His Gln Tyr Asp Trp Pro Gln Glu Val Asn
275 280 285
Phe Lys Val Arg Glu Met Leu Asp Ile His Arg Leu Ile Trp Arg Arg
290 295 300
His Tyr Gln Asn Leu Lys Asp Ser Asp Phe Ser Phe Val Glu Gly Thr
305 310 315 320
His Glu Gln Ser Leu Gln Ala Leu Asn Pro Ser Cys Leu Ala Leu Leu
325 330 335
Asn His Leu Arg Ile Tyr Ile Pro Lys Leu Phe Pro Asp Leu Asn Lys
340 345 350
Ile Val Leu Leu Asp Asp Asp Val Val Val Gln Ser Asp Leu Ser Ser
355 360 365
Leu Trp Glu Thr Asp Leu Asn Gly Lys Val Val Gly Ala Val Val Asp
370 375 380
Ser Trp Cys Gly Asp Asn Cys Cys Pro Gly Arg Lys Tyr Lys Asp Tyr
385 390 395 400
Phe Asn Phe Ser His Pro Leu Ile Ser Ser Asn Leu Val Gln Glu Asp
405 410 415
Cys Ala Trp Leu Ser Gly Met Asn Val Phe Asp Leu Lys Ala Trp Arg
420 425 430
Gln Thr Asn Ile Thr Glu Ala Tyr Ser Thr Trp Leu Arg Leu Ser Val
435 440 445
Arg Ser Gly Leu Gln Leu Trp Gln Pro Gly Ala Leu Pro Pro Thr Leu
450 455 460
Leu Ala Phe Lys Gly Leu Thr Gln Ser Leu Glu Pro Ser Trp His Val
465 470 475 480
Ala Gly Leu Gly Ser Arg Ser Val Lys Ser Pro Gln Glu Ile Leu Lys
485 490 495
Ser Ala Ser Val Leu His Phe Ser Gly Pro Ala Lys Pro Trp Leu Glu
500 505 510
Ile Ser Asn Pro Glu Val Arg Ser Leu Trp Tyr Arg Tyr Val Asn Ser
515 520 525
Ser Asp Ile Phe Val Arg Lys Cys Lys Ile Met Asn
530 535 540
<210> 223
<211> 525
<212> PRT
<213> 拟南芥()
<220>
<223> 木聚糖生物合成酶不规则的木质部14 (IRX14) GI:30690793
<400> 223
Met Lys Leu Ser Ala Leu His Gln Ser Tyr Leu Asn Arg Arg Ser Asn
1 5 10 15
Ser Phe Arg Ser Pro Thr Ser Leu Asp Ser Ser Val Asp Gly Ser Gly
20 25 30
Lys Ser Leu Ile Ala Val Phe Trp Leu Ile Leu His Cys Leu Cys Cys
35 40 45
Leu Ile Ser Leu Val Leu Gly Phe Arg Phe Ser Arg Leu Val Phe Phe
50 55 60
Phe Leu Phe Ser Thr Ser Ser Thr Asn Leu Tyr Ser Leu Pro Phe Arg
65 70 75 80
Pro Asp Leu Pro Val Lys His Leu Asp Val His Thr Ile Gly Arg Thr
85 90 95
Leu Asp Pro Gly Ala Asn Gly Thr Thr Val Val Ala Thr Ala Thr Lys
100 105 110
Ser Ser Arg Val Val Val Gly Arg His Gly Ile Arg Ile Arg Pro Trp
115 120 125
Pro His Pro Asn Pro Val Glu Val Met Lys Ala His Gln Ile Ile Gly
130 135 140
Arg Val Gln Lys Glu Gln Lys Met Ile Phe Gly Met Lys Ser Ser Lys
145 150 155 160
Met Val Ile Ala Val Thr Pro Thr Tyr Val Arg Thr Phe Gln Ala Leu
165 170 175
His Leu Thr Gly Val Met His Ser Leu Met Leu Val Pro Tyr Asp Leu
180 185 190
Val Trp Ile Val Val Glu Ala Gly Gly Ala Thr Asn Glu Thr Gly Leu
195 200 205
Ile Ile Ala Lys Ser Gly Leu Arg Thr Ile His Val Gly Ile Asp Gln
210 215 220
Arg Met Pro Asn Thr Trp Glu Asp Arg Ser Lys Leu Glu Val Phe Met
225 230 235 240
Arg Leu Gln Ala Leu Arg Val Val Arg Glu Glu Lys Leu Asp Gly Ile
245 250 255
Val Met Phe Ala Asp Asp Ser Asn Met His Ser Met Glu Leu Phe Asp
260 265 270
Glu Ile Gln Asn Val Lys Trp Phe Gly Thr Val Ser Val Gly Ile Leu
275 280 285
Ala His Ser Gly Asn Ala Glu Glu Met Val Leu Ser Met Glu Lys Arg
290 295 300
Lys Glu Met Glu Lys Glu Glu Glu Glu Glu Ser Ser Ser Leu Pro Val
305 310 315 320
Gln Gly Pro Ala Cys Asn Ser Thr Asp Gln Leu Ile Gly Trp His Ile
325 330 335
Phe Asn Thr Leu Pro Tyr Ala Gly Lys Ser Ala Val Tyr Ile Asp Asp
340 345 350
Val Ala Ala Val Leu Pro Gln Lys Leu Glu Trp Ser Gly Phe Val Leu
355 360 365
Asn Ser Arg Leu Leu Trp Glu Glu Ala Glu Asn Lys Pro Glu Trp Val
370 375 380
Lys Asp Phe Gly Ser Leu Asn Glu Asn Glu Gly Val Glu Ser Pro Leu
385 390 395 400
Ser Leu Leu Lys Asp Pro Ser Met Val Glu Pro Leu Gly Ser Cys Gly
405 410 415
Arg Gln Val Leu Leu Trp Trp Leu Arg Val Glu Ala Arg Ala Asp Ser
420 425 430
Lys Phe Pro Pro Gly Trp Ile Ile Asp Pro Pro Leu Glu Ile Thr Val
435 440 445
Ala Ala Lys Arg Thr Pro Trp Pro Asp Val Pro Pro Glu Pro Pro Thr
450 455 460
Lys Lys Lys Asp Gln Met Pro Leu Ser Gln Gly Asn Thr Val Val Val
465 470 475 480
Ile Pro Lys Gln Gln Gln His Pro Thr Lys Ile Arg Lys Pro Lys Arg
485 490 495
Lys Ser Lys Lys Ser Lys His Glu Pro Arg Pro Thr Asp Thr Thr Thr
500 505 510
Gln Val Tyr Ser Ser Ser Ser Lys His Gln Glu Arg Asn
515 520 525
<210> 224
<211> 492
<212> PRT
<213> 拟南芥()
<220>
<223> 木聚糖生物合成酶不规则的木质部14 (IRX14) GI:15240245
<400> 224
Met Lys Leu Ser Val Phe Arg Leu Ser Tyr Trp Asn Arg Arg Gly Ser
1 5 10 15
Ser Phe Arg Ser Ser Pro Ser Leu Asp Pro Ser Phe Asp Gly Lys Ser
20 25 30
Pro Ser Ser Val Phe Trp Phe Val Ile His Gly Leu Cys Cys Leu Ile
35 40 45
Ser Leu Ile Leu Gly Phe Arg Phe Ser His Leu Val Leu Phe Phe Leu
50 55 60
Phe Ser Thr Ser Val Thr Asn Leu Tyr Thr Thr Pro Phe Leu Phe Ala
65 70 75 80
Gly Asn Gly Gly Val Ser Gln Leu Leu Arg Leu Lys Pro Leu Glu Thr
85 90 95
Ala Thr Asn Ser Thr Val Lys Lys Asn Ser Arg Val Val Val Gly Arg
100 105 110
His Gly Ile Arg Ile Arg Pro Trp Pro His Pro Asn Pro Ile Glu Val
115 120 125
Leu Arg Ala His Gln Leu Leu Val Arg Val Gln Lys Glu Gln Lys Ser
130 135 140
Met Tyr Gly Val Arg Ser Pro Arg Thr Val Ile Val Val Thr Pro Thr
145 150 155 160
Tyr Val Arg Thr Phe Gln Ala Leu His Leu Thr Gly Val Met His Ser
165 170 175
Leu Met Leu Val Pro Tyr Asp Leu Val Trp Ile Val Val Glu Ala Gly
180 185 190
Gly Ile Thr Asn Glu Thr Ala Ser Phe Ile Ala Lys Ser Gly Leu Lys
195 200 205
Thr Ile His Leu Gly Phe Asp Gln Lys Met Pro Asn Thr Trp Glu Asp
210 215 220
Arg His Lys Leu Glu Thr Lys Met Arg Leu His Ala Leu Arg Val Val
225 230 235 240
Arg Glu Lys Lys Leu Asp Gly Ile Val Met Phe Ala Asp Asp Ser Asn
245 250 255
Met His Ser Met Glu Leu Phe Asp Glu Ile Gln Thr Val Lys Trp Phe
260 265 270
Gly Ala Leu Ser Val Gly Ile Leu Ala His Ser Gly Asn Ala Asp Glu
275 280 285
Leu Ser Ser Ile Leu Lys Asn Glu Gln Gly Lys Asn Lys Glu Lys Pro
290 295 300
Ser Met Pro Ile Gln Gly Pro Ser Cys Asn Ser Ser Glu Lys Leu Val
305 310 315 320
Gly Trp His Ile Phe Asn Thr Gln Pro Tyr Ala Lys Lys Thr Ala Val
325 330 335
Tyr Ile Asp Glu Lys Ala Pro Val Met Pro Ser Lys Met Glu Trp Ser
340 345 350
Gly Phe Val Leu Asn Ser Arg Leu Leu Trp Lys Glu Ser Leu Asp Asp
355 360 365
Lys Pro Ala Trp Val Lys Asp Leu Ser Leu Leu Asp Asp Gly Tyr Ala
370 375 380
Glu Ile Glu Ser Pro Leu Ser Leu Val Lys Asp Pro Ser Met Val Glu
385 390 395 400
Pro Leu Gly Ser Cys Gly Arg Arg Val Leu Leu Trp Trp Leu Arg Val
405 410 415
Glu Ala Arg Ala Asp Ser Lys Phe Pro Pro Gly Trp Ile Ile Lys Ser
420 425 430
Pro Leu Glu Ile Thr Val Pro Ser Lys Arg Thr Pro Trp Pro Asp Ser
435 440 445
Ser Ser Glu Leu Pro Ala Ala Ala Ile Lys Glu Ala Lys Ser Asn Ser
450 455 460
Lys Pro Arg Val Ser Lys Ser Lys Ser Tyr Lys Glu Lys Gln Glu Pro
465 470 475 480
Lys Ala Phe Asp Gly Val Lys Val Ser Ala Thr Ser
485 490
<210> 225
<211> 510
<212> PRT
<213> 毛果杨()
<220>
<223> 木聚糖生物合成酶不规则的木质部14 (IRX14) 同系物 GI:224096716
<400> 225
Met Lys Phe Ser Leu Leu Gln Gln Ser Tyr Asn Asn Arg Arg Ser Gly
1 5 10 15
Ser Phe Arg Gly Ser Ser Ala Pro Leu Asp Ser Ser Pro Asp Asn Thr
20 25 30
Ile Lys Ser Pro Ala Ala Ile Phe Trp Leu Phe Leu His Gly Ile Cys
35 40 45
Cys Leu Ile Ser Leu Val Leu Gly Phe Arg Phe Ser Arg Leu Val Phe
50 55 60
Phe Phe Leu Phe Ser Thr Ser Thr Thr Thr Thr Leu Tyr Val Thr Thr
65 70 75 80
Pro Phe His Pro Leu Ser Lys Thr Ser Asp Ile Ser Asn Pro Leu Thr
85 90 95
Asn Ser Ala Asn Asp Leu Pro Val Ile Asn Lys Thr Val Ser Ser Arg
100 105 110
Val Val Val Gly Arg His Gly Ile Arg Ile Arg Pro Trp Pro His Pro
115 120 125
Asn Pro Ser Glu Val Ile Lys Ala His Gln Ile Ile Glu Arg Val Gln
130 135 140
Arg Glu Gln Ser Asn Gln Phe Gly Val Lys Ser Pro Arg Ser Leu Ile
145 150 155 160
Val Val Thr Pro Thr Tyr Val Arg Thr Phe Gln Thr Leu His Met Thr
165 170 175
Gly Val Met His Ser Leu Met Leu Leu Pro Tyr Asp Val Val Trp Ile
180 185 190
Val Val Glu Ala Gly Gly Val Thr Asn Glu Thr Ala Leu Ile Ile Ala
195 200 205
Lys Ser Gly Val Lys Thr Leu His Ile Gly Phe Asn Gln Lys Met Pro
210 215 220
Asn Ser Trp Glu Gly Arg His Arg Leu Glu Thr Lys Met Arg Leu Arg
225 230 235 240
Ala Leu Arg Val Val Arg Glu Glu Lys Met Asp Gly Ile Val Met Phe
245 250 255
Ala Asp Asp Ser Asn Met His Ser Met Glu Leu Phe Asp Glu Ile Gln
260 265 270
Asn Val Lys Trp Phe Gly Ala Val Ser Val Gly Ile Leu Val His Ser
275 280 285
Gly Gly Ala Asp Glu Thr Leu Leu Thr Ala Ala Ala Ala Met Val Asp
290 295 300
Lys Glu Ala Glu Glu Asn Leu Pro Asn Pro Val Val Pro Val Gln Gly
305 310 315 320
Pro Ala Cys Asn Ala Ser Asn Lys Leu Val Gly Trp His Thr Phe Asn
325 330 335
Ser Leu Pro Tyr Glu Gly Lys Ser Ala Val Tyr Ile Asp Asp Arg Ala
340 345 350
Thr Val Leu Pro Arg Lys Leu Glu Trp Ala Gly Phe Met Leu Asn Ser
355 360 365
Arg Leu Leu Trp Lys Glu Ala Glu Asp Lys Pro Glu Trp Val Lys Asp
370 375 380
Met Asp Leu Val Asp Glu Asn Ile Glu Asn Pro Leu Ala Leu Leu Lys
385 390 395 400
Asp Pro Ser Met Val Glu Pro Leu Gly Ser Cys Gly Arg Gln Val Leu
405 410 415
Leu Trp Trp Leu Arg Val Glu Ala Arg Ala Asp Ser Lys Phe Pro Pro
420 425 430
Gly Trp Ile Ile Asp Pro Pro Leu Glu Ile Thr Val Pro Ser Lys Arg
435 440 445
Thr Pro Trp Pro Asp Ala Pro Pro Glu Leu Pro Ser Asn Glu Lys Ile
450 455 460
Ser Val Asn Gln Glu Gln Thr Ala Lys Arg Ser Ser Lys Thr Arg Ser
465 470 475 480
Pro Arg Ser Lys Arg Ser Ser Arg Ser Lys Arg Lys His Glu Val Val
485 490 495
Leu Ala Glu Thr Gln Val Ser Ala Arg His Ser Glu Gln Asn
500 505 510
<210> 226
<211> 503
<212> PRT
<213> 毛果杨()
<220>
<223> 木聚糖生物合成酶不规则的木质部14 (IRX14) 同系物 GI:224081752
<400> 226
Met Lys Leu Ser Met Leu Gln Gln Ser Tyr Met Asn Arg Arg Ser Ala
1 5 10 15
Ser Phe Arg Gly Ser Ser Ala Pro Leu Asp Ser Ser Thr Asp Asn Thr
20 25 30
Ile Lys Ser Pro Ala Ala Ile Phe Trp Leu Leu Leu His Gly Phe Cys
35 40 45
Cys Leu Ile Ser Leu Val Leu Gly Phe Arg Phe Ser Arg Leu Val Phe
50 55 60
Phe Phe Leu Phe Ser Thr Ser Thr Thr Thr Thr Leu Tyr Ile Ala Thr
65 70 75 80
Pro Leu Pro His Leu Thr Lys Thr Asn Asn Asn Ile Asn Asp Leu Pro
85 90 95
Leu Glu Ile Pro Val Ile Asn Lys Thr Leu Ser Ser Ser Ser Arg Val
100 105 110
Val Val Gly Arg His Gly Ile Arg Ile Arg Pro Trp Pro His Pro Asn
115 120 125
Pro Ser Glu Val Met Lys Ala His Gln Ile Ile Glu Thr Val Gln Arg
130 135 140
Glu Gln Arg Thr Gln Phe Gly Val Lys Ser Pro Arg Thr Leu Ile Val
145 150 155 160
Val Thr Pro Thr Tyr Val Arg Thr Phe Gln Thr Leu His Leu Thr Gly
165 170 175
Val Met His Ser Leu Met Leu Val Pro Tyr Asp Val Val Trp Ile Val
180 185 190
Val Glu Ala Gly Gly Ala Thr Asn Glu Thr Ala Ser Ile Ile Ala Lys
195 200 205
Ser Ser Ile Lys Thr Phe His Ile Gly Phe Thr Gln Lys Met Pro Asn
210 215 220
Ser Trp Glu Gly Arg His Lys Leu Glu Thr Lys Met Arg Leu Arg Ala
225 230 235 240
Leu Arg Val Val Arg Glu Glu Met Met Asp Gly Ile Val Met Phe Ala
245 250 255
Asp Asp Ser Asn Met His Ser Met Glu Leu Phe Asp Glu Ile Gln Asn
260 265 270
Val Lys Trp Phe Gly Ala Val Ser Val Gly Ile Leu Ala His Ser Gly
275 280 285
Gly Gly Gly Glu Ser Ser Ser Ala Val Ala Glu Lys Asp Val Lys Pro
290 295 300
Asn Leu Ser Asn Pro Ala Met Pro Val Gln Gly Pro Ala Cys Asn Ala
305 310 315 320
Ser Asn Lys Leu Val Gly Trp His Thr Phe Asp Ser Leu Pro Tyr Glu
325 330 335
Gly Lys Ser Ala Val Tyr Ile Asp Asp Arg Ala Thr Val Leu Pro Arg
340 345 350
Lys Leu Glu Trp Ala Gly Phe Val Leu Asn Ser Arg Leu Leu Leu Lys
355 360 365
Glu Ala Gln Asp Lys Pro Glu Trp Val Lys Asp Leu Asp Leu Val Asp
370 375 380
Glu Asn Ile Glu Ser Pro Leu Ala Leu Leu Lys Asp Pro Ser Met Val
385 390 395 400
Glu Pro Leu Gly Ser Cys Gly Arg Gln Val Leu Leu Trp Trp Leu Arg
405 410 415
Val Glu Ala Arg Ala Asp Ser Lys Phe Pro Pro Gly Trp Ile Ile Asp
420 425 430
Pro Pro Leu Glu Ile Thr Val Pro Ser Lys Arg Thr Pro Trp Pro Asp
435 440 445
Ala Pro Pro Glu Leu Pro Ser Asn Lys Lys Leu Thr Ile Asn Gln Glu
450 455 460
Gln Thr Ile Lys Arg Ser Pro Lys Thr Arg Ser Pro Arg Ser Lys Arg
465 470 475 480
Arg Ser Lys Arg Lys His Glu Ala Lys Leu Val Glu Thr Gln Val Ser
485 490 495
Thr Arg His Ser Glu Gln Asn
500
<210> 227
<211> 502
<212> PRT
<213> 江南卷柏()
<220>
<223> 木聚糖生物合成酶不规则的木质部14 (IRX14) 同系物 GI:302797519
<400> 227
Met Lys Ser Gly Ser Ala Ala Ala Gly Gly Val Leu His Pro Val Leu
1 5 10 15
Arg Arg Ser Leu Thr Asn Ala Ala Ser Thr Pro Arg Lys Ser Ser Gly
20 25 30
Ser Ser Ser Pro Glu Arg Gly Gly Gly Gly Gly Val Leu Ile Gly Pro
35 40 45
Gly Pro Leu Ile Arg Leu Cys Leu His Ala Val Ala Cys Ile Ala Ser
50 55 60
Leu Val Ile Gly Phe Arg Phe Ser Arg Glu Thr Leu Leu Val Leu Leu
65 70 75 80
Ile Asp Val Gly Ile Lys Phe Pro Tyr Val Glu Pro Gly Leu Gly Val
85 90 95
Arg Asp Gly Val Gln Asp Gly Gly Gly Arg Leu Pro Glu Val Arg Thr
100 105 110
Lys Ser Gly Arg Val His Val Gly Arg His Glu Ile Leu Ile Arg Pro
115 120 125
Trp Pro His Pro Asp Ser Ala Glu Ile Tyr Arg Ala His Ala Leu Leu
130 135 140
Glu Arg Val Gln Val Glu Gln Gln Ser Leu Tyr Gly Pro Lys Glu Arg
145 150 155 160
Lys Thr Val Ile Ala Ile Thr Pro Thr Phe Ala Arg Thr Phe Gln Ala
165 170 175
Ile His Leu Leu Gly Val Met His Thr Leu Arg Ala Ala Pro Gly Pro
180 185 190
Val Ile Trp Ile Val Val Glu Ala Gly Gly Arg Ser Asn Glu Thr Ala
195 200 205
Ser Ile Leu Ala Ser Ser Arg Leu Glu Phe Val His Leu Gly Val Lys
210 215 220
Asp Ala Met Pro Val Ala Trp Glu Gln Arg Arg Arg Met Glu Thr Arg
225 230 235 240
Leu Arg Ile Glu Gly Leu Ser His Val Arg Arg Glu Lys Leu Asp Gly
245 250 255
Leu Ile Leu Phe Ala Asp Asp Ser Asn Val His Ser Leu Gln Leu Phe
260 265 270
Asp Glu Ile Gln Lys Val Lys Trp Ile Gly Ala Leu Ser Val Gly Leu
275 280 285
Leu Glu Thr Gly Ser Gly Ala Thr Glu Thr Ala Ser Ser Met Val Ala
290 295 300
Ala Ala Ser Ser Ala Lys Pro Arg Leu Pro Val Gln Gly Pro Ala Cys
305 310 315 320
Asn Glu Thr Cys His Val Val Gly Trp His Val Leu Arg Pro Ser Pro
325 330 335
Val Asp Gly Glu Asp Asp Ser Ser Ser Ser Ser Phe Thr Asp Val Ala
340 345 350
Gly Gly Leu Thr Asp Val Ala Thr His Leu Glu Trp Ser Gly Phe Val
355 360 365
Ile Asn Ser Arg Ala Val Trp Asp Glu Ala Glu Ser Glu Asn Glu Glu
370 375 380
Glu Glu Ala Ser Ser Ser Thr Met Glu Asp Glu Gln Gln Glu Glu Asp
385 390 395 400
Ala Thr Arg Arg Lys Pro Glu Trp Ile Asn Glu Trp Lys Glu Trp Ile
405 410 415
Ser Glu Asp Ser Gly Ser Pro Leu Arg Leu Ala Lys Asp Gln Gly Phe
420 425 430
Ile Glu Ala Leu Gly Asn Cys Gly Arg Glu Val Met Leu Trp Trp Ile
435 440 445
Arg Val Glu Ala Arg Ala Asp Ser Arg Tyr Pro Pro Arg Gln Val Ala
450 455 460
Leu Cys Pro Thr Arg Gly Tyr His Ile Tyr Gln Leu Thr Arg Leu Pro
465 470 475 480
His Arg Trp Ser Leu Thr Ser Pro Leu Glu Ile Thr Val Pro Ala Gln
485 490 495
Arg Thr Pro Trp Pro Glu
500
<210> 228
<211> 263
<212> PRT
<213> 水稻()
<220>
<223> 木聚糖生物合成酶不规则的木质部14 (IRX14) 同系物 GI:115469624
<400> 228
Met Pro His Asp Trp Ala Asp Arg His Ala Thr Glu Asn Arg Met Arg
1 5 10 15
Leu His Ala Leu Arg Val Ile Arg Glu Arg Lys Met Asp Gly Val Ile
20 25 30
Val Phe Ala Asp Asp Ser Asn Val His Ser Leu Glu Leu Phe Asp Glu
35 40 45
Val Gln Lys Val Gln Trp Met Gly Ala Val Ser Val Gly Ile Leu Ala
50 55 60
His Thr Gly Thr Ala Asp Gln Pro Arg Leu Ser Glu Glu Asp Lys Gln
65 70 75 80
Asn Met Pro Leu Pro Val Gln Gly Pro Ala Cys Asn Ser Ser Gly His
85 90 95
Leu Ala Gly Trp His Thr Phe Asn Ser Leu Pro Phe Ala Gly Lys Thr
100 105 110
Ala Thr Val Val Gly Glu Ala Ala Pro Val Leu Pro Arg Gly Leu Glu
115 120 125
Trp Ala Gly Phe Val Leu Asn Ser Arg Ile Leu Trp Lys Glu Ala Glu
130 135 140
Gly Lys Pro Asp Trp Val Lys Asp Leu Asp Ala Val Gly Glu Asn Gly
145 150 155 160
Glu Glu Ile Glu Asn Pro Leu Ile Leu Leu Asn Asp Pro Ser Ser Val
165 170 175
Glu Pro Leu Gly Asn Cys Gly Lys Lys Ile Leu Leu Trp Trp Leu Arg
180 185 190
Val Glu Ala Arg Ala Asp Ser Lys Phe Pro Gln Gly Trp Val Ile Glu
195 200 205
Pro Pro Leu Asp Ile Val Val Pro Ala Lys Arg Thr Pro Trp Pro Glu
210 215 220
Thr Thr Ala Glu Leu Ser Ala Glu Leu Val Asp Ser Lys Gln Asp Gln
225 230 235 240
Glu Gly Arg Arg Leu Ser Arg Thr Asp Arg Ser Ser Arg Ser Arg Ser
245 250 255
Thr Thr Lys Arg Lys Glu Asn
260
<210> 229
<211> 351
<212> PRT
<213> 拟南芥()
<220>
<223> 木聚糖生物合成酶不规则的木质部9 (IRX9) GI:15228084
<400> 229
Met Gly Ser Leu Glu Arg Ser Lys Lys Lys Ala Gln Val Trp Lys Lys
1 5 10 15
Ala Val Ile His Phe Ser Leu Cys Phe Val Met Gly Phe Phe Thr Gly
20 25 30
Phe Ala Pro Ala Gly Lys Ala Ser Phe Phe Ser Asn Phe Glu Thr Thr
35 40 45
Ser Tyr Thr Ser Thr Lys Ser Pro Ile Pro Pro Gln Pro Phe Glu Asn
50 55 60
Ala Thr Tyr Thr Gln His Ser Leu Leu Asn Arg Thr Leu Ile Asn Ser
65 70 75 80
Gln Ser Gln Ala Pro Ala Pro Ala Glu Ser Arg Glu Ala Glu Gly Glu
85 90 95
Thr Arg Ser Leu Ser Glu Lys Glu Asp Glu Asn Gln Val Lys Val Thr
100 105 110
Pro Arg Gly Leu Val Ile Val Val Thr Pro Ile Ile Thr Lys Asp Arg
115 120 125
Tyr Lys Asn Val Leu Leu Arg Arg Met Ala Asn Thr Leu Arg Leu Val
130 135 140
Pro Pro Pro Leu Leu Trp Ile Val Val Glu Lys His Ser Asp Gly Glu
145 150 155 160
Glu Lys Ser Ser Ser Thr Met Leu Arg Lys Thr Gly Ile Met Tyr Arg
165 170 175
Arg Ile Val Phe Lys Glu Asp Phe Thr Ser Leu Glu Ser Glu Leu Asp
180 185 190
His Gln Arg Asn Leu Ala Leu Arg His Ile Glu His His Lys Leu Ser
195 200 205
Gly Ile Val His Phe Ala Gly Leu Asn Asn Ile Tyr Asp Leu Asp Phe
210 215 220
Phe Val Lys Ile Arg Asp Ile Glu Val Phe Gly Thr Trp Pro Met Ala
225 230 235 240
Leu Leu Ser Ala Asn Arg Lys Arg Val Val Val Glu Gly Pro Val Cys
245 250 255
Glu Ser Ser Gln Val Leu Gly Trp His Leu Arg Lys Ile Asn Asn Glu
260 265 270
Thr Glu Thr Lys Pro Pro Ile His Ile Ser Ser Phe Ala Phe Asn Ser
275 280 285
Ser Ile Leu Trp Asp Pro Glu Arg Trp Gly Arg Pro Ser Ser Val Glu
290 295 300
Gly Thr Lys Gln Asp Ser Ile Lys Tyr Val Lys Gln Val Val Leu Glu
305 310 315 320
Asp Asp Thr Lys Leu Lys Gly Leu Pro Ala Gln Asp Cys Ser Lys Ile
325 330 335
Met Leu Trp Arg Leu Lys Phe Pro Thr Arg Thr Arg Leu Ser Thr
340 345 350
<210> 230
<211> 357
<212> PRT
<213> 毛果杨()
<220>
<223> 木聚糖生物合成酶不规则的木质部9 (IRX9) 同系物 GI:224140167
<400> 230
Met Gly Ser Val Glu Arg Ser Lys Arg Arg Val Gln Leu Trp Lys Lys
1 5 10 15
Ala Ile Val His Phe Gly Leu Cys Phe Val Met Gly Phe Phe Thr Gly
20 25 30
Phe Ala Pro Ala Gly Lys Ala Ser Ile Phe Thr Ser His Val Ala Ala
35 40 45
Ser Asn Lys Ser Gln Ser Leu Pro Gln Pro Val Glu Met Thr Leu His
50 55 60
Gln Gln Ala Ala Ser Thr Pro His Ala Ser Asn Val Asn Arg Ser Leu
65 70 75 80
Ile Ala Glu Thr Ala Val Pro Ala Pro Pro Ser Ser Lys Glu Ser Glu
85 90 95
His Ala Thr Phe Leu Gly Lys Glu Glu Thr Glu Ser Lys Leu Ala Pro
100 105 110
Arg Arg Leu Ala Ile Ile Val Thr Pro Thr Ser Thr Lys Asp Pro Tyr
115 120 125
Gln Gly Val Phe Leu Arg Arg Leu Ala Asn Thr Ile Arg Leu Val Pro
130 135 140
Pro Pro Leu Leu Trp Ile Val Val Glu Gly Gln Ser Asp Ser Asp Glu
145 150 155 160
Val Ser Glu Val Leu Arg Lys Thr Gly Ile Met Tyr Arg His Leu Val
165 170 175
Phe Lys Glu Asn Phe Thr Asp Pro Glu Ala Glu Leu Asp His Gln Arg
180 185 190
Asn Val Ala Leu Arg His Ile Glu Lys His Arg Leu Ser Gly Ile Val
195 200 205
His Phe Ala Gly Leu Ser Asn Val Tyr Asp Leu Gly Phe Phe Asp Glu
210 215 220
Ile Arg Gln Ile Glu Val Phe Gly Thr Trp Pro Met Ala Leu Leu Ser
225 230 235 240
Ala Asn Glu Lys Lys Val Ile Ile Glu Gly Pro Val Cys Asp Ser Ser
245 250 255
Gln Val Ile Gly Trp His Leu Arg Lys Met Asn Asn Glu Thr Asp Lys
260 265 270
Arg Pro Pro Ile His Ile Ser Ser Phe Gly Phe Asn Ser Ser Ile Leu
275 280 285
Trp Asp Pro Glu Arg Trp Gly Arg Pro Ser Ser Val Gln Gln Thr Ser
290 295 300
Gln Asn Ser Ile Lys Phe Val Lys Gln Val Ala Leu Glu Asp Glu Thr
305 310 315 320
Lys Leu Lys Gly Ile Pro Pro Glu Asp Cys Ser Lys Ile Met Leu Trp
325 330 335
Arg Leu Asn Leu Pro Thr Ser Lys Ser Pro Ser Tyr Gln Glu Asn Gln
340 345 350
Glu Asp Lys Ile Val
355
<210> 231
<211> 359
<212> PRT
<213> 毛果杨()
<220>
<223> 木聚糖生物合成酶不规则的木质部9 (IRX9) 同系物 GI:224069352
<400> 231
Met Gly Ser Leu Glu Arg Ser Lys Lys Lys Val Gln Leu Trp Lys Lys
1 5 10 15
Ala Ile Val His Phe Gly Leu Cys Phe Val Met Gly Phe Phe Thr Gly
20 25 30
Phe Ala Pro Gly Gly Lys Ala Ser Ile Phe Ser Ser His Val Val Ala
35 40 45
Ser Asn Lys Ser Gln Pro Val Glu Met Leu His Gln Gln Val Ala Ser
50 55 60
Thr Pro His Ala Ser Asn Val Asn Arg Ser Leu Ile Ala Glu Ser Pro
65 70 75 80
Val Pro Thr Pro Leu Ser Ser Lys Glu Ser Glu Pro Ala Lys Phe Leu
85 90 95
Glu Lys Glu Glu Glu Pro Lys Pro Lys Leu Leu Pro Arg Arg Leu Ala
100 105 110
Ile Ile Val Thr Pro Ile Ser Thr Glu Asp Pro Tyr Gln Gly Val Phe
115 120 125
Leu Arg Arg Leu Ala Asn Thr Ile Arg Leu Val Pro Pro Pro Leu Leu
130 135 140
Trp Ile Val Val Glu Gly Gln Ser Asp Ser Asp Glu Val Ser Glu Ile
145 150 155 160
Leu Arg Lys Thr Gly Ile Met Tyr Arg His Leu Val Ile Lys Glu Asn
165 170 175
Phe Thr Asp Pro Glu Ala Glu Leu Asp His Gln Arg Asn Val Ala Leu
180 185 190
Arg His Ile Glu Gln His Arg Leu Ser Gly Ile Val His Phe Ala Gly
195 200 205
Leu Ser Asn Val Tyr Asp Leu Gly Phe Phe Asp Glu Leu Arg Gln Ile
210 215 220
Glu Val Phe Gly Thr Trp Pro Val Ala Leu Leu Ser Ala Asn Lys Asn
225 230 235 240
Lys Val Thr Ile Glu Gly Pro Val Cys Asp Ser Ser Gln Val Ile Gly
245 250 255
Trp His Leu Lys Lys Met Asn Asn Glu Thr Asp Lys Arg Pro Pro Ile
260 265 270
His Ile Ser Ser Phe Gly Phe Asn Ser Ser Ile Leu Trp Asp Pro Glu
275 280 285
Arg Trp Gly Arg Pro Ser Ser Val Gln Gln Thr Ser Gln Asn Ser Ile
290 295 300
Lys Phe Val Lys Gln Ala Ala Leu Glu Asp Glu Thr Glu Leu Lys Gly
305 310 315 320
Ile Pro Pro Glu Asp Cys Ser Lys Ile Met Leu Trp Arg Leu Asn Leu
325 330 335
Pro Val Ser Lys Ser Pro Ser Tyr His Leu Ser Thr Thr Gly Ser Thr
340 345 350
Asp Ala Ser Arg Arg Lys Ile
355
<210> 232
<211> 339
<212> PRT
<213> 水稻()
<220>
<223> 木聚糖生物合成酶不规则的木质部9 (IRX9) 同系物 GI:297600755
<400> 232
Met Gly Ser Ser Thr Asp His Gly Gly Ala Gly Gly Arg Gly Lys Lys
1 5 10 15
Gly Ser Gly Ser Gln Leu Trp Lys Lys Ala Leu Leu His Ser Ser Leu
20 25 30
Cys Phe Val Met Gly Phe Phe Thr Gly Phe Ala Pro Ser Ser Val Ser
35 40 45
Asp Trp Thr Ser Ala Ala Val Ser Ala Gly Gly Val Gly Ser Ser His
50 55 60
Val Val Arg Ser Leu His Ala Thr Gly Gly Ala Ala Val Asn Arg Ser
65 70 75 80
Leu Leu Ala Gln Ala Ala Ala Gly Ala Val Asp Ala Gly Pro Gln Pro
85 90 95
Leu Leu Val Val Val Thr Thr Thr Glu Ser Thr Pro Ser Ala Ala Gly
100 105 110
Gln Arg Ala Ala Ala Leu Thr Arg Met Ala His Thr Leu Arg Leu Val
115 120 125
Pro Pro Pro Leu Leu Trp Val Val Val Glu Ala Asn Pro Asp Val Ala
130 135 140
Ala Thr Ala Arg Leu Leu Arg Thr Thr Gly Leu Met Tyr Arg His Leu
145 150 155 160
Thr Tyr Lys Asp Asn Phe Thr Val Ala Asp Ala Ala Ala Gly Lys Glu
165 170 175
Arg His His Gln Arg Asn Val Ala Leu Gly His Ile Glu His His Arg
180 185 190
Leu Ala Gly Val Val Leu Phe Ala Gly Leu Gly Asp Thr Phe Asp Leu
195 200 205
Arg Phe Phe Asp Gln Leu Arg Gln Ile Arg Thr Phe Gly Ala Trp Pro
210 215 220
Val Ala Thr Met Ser Gln Asn Glu Arg Lys Val Val Val Gln Gly Pro
225 230 235 240
Ala Cys Ser Ser Ser Ser Val Ala Gly Trp Phe Ser Met Asp Leu Ser
245 250 255
Asn Ala Thr Ser Pro Val Ala Val Gly Gly Ala Gly Tyr Gly Ala Ala
260 265 270
Ala Ala Arg Pro Arg Glu Leu Asp Val His Gly Phe Ala Phe Asn Ser
275 280 285
Ser Val Leu Trp Asp Pro Glu Arg Trp Gly Arg Tyr Pro Thr Ser Glu
290 295 300
Pro Asp Lys Ser Gln Asp Ser Val Lys Phe Val Gln Gln Val Val Leu
305 310 315 320
Glu Asp Tyr Ser Lys Val Arg Gly Ile Pro Ser Asp Cys Ser Glu Val
325 330 335
Met Arg Val
<210> 233
<211> 371
<212> PRT
<213> 水稻()
<220>
<223> 木聚糖生物合成酶不规则的木质部9 (IRX9) 同系物 GI:115461821
<400> 233
Met Gly Thr Ala Ala Val Ala Ala Ala Glu Arg Pro Lys Gln Arg Arg
1 5 10 15
Ser Ser His Leu Trp Lys Lys Ala Leu Leu His Phe Ser Leu Cys Phe
20 25 30
Val Met Gly Phe Phe Thr Gly Phe Ala Pro Ser Ser Ser Ser Ser Trp
35 40 45
Arg Ala Gly Ser Gly Gly Gly Gly Gly Val Gln Pro Arg His Gln Leu
50 55 60
Ala Ala Ser His Val Ala Val Asn Gln Gln Val Ser Leu Val Pro Asp
65 70 75 80
Ala Ala Ala Ala Glu Ala Ala Gly Val Gly Asn Gly Ala Val Val Asp
85 90 95
Val Gly Asp Asp Glu Gly Gly Glu Gly Ala Arg Arg Met Leu Ile Val
100 105 110
Val Thr Thr Thr Arg Gly Glu Arg Arg Arg Arg Arg Gly Glu Leu Leu
115 120 125
Arg Leu Ala His Thr Leu Arg Leu Val Arg Pro Pro Val Val Trp Val
130 135 140
Val Val Glu Pro Ala Ala Asp Ala Ala Ala Thr Ala Glu Val Leu Arg
145 150 155 160
Gly Thr Gly Val Met Tyr Arg His Leu Ala Phe Arg Pro Glu Glu Asn
165 170 175
Phe Thr Thr Ala Asp Ala Glu Ala His Ala Gln Arg Asn Ala Ala Leu
180 185 190
Ala His Val Glu Lys His Arg Leu Ser Gly Val Val His Phe Ala Asp
195 200 205
Ala Ala Gly Val Tyr Asp Ala His Phe Phe Asp Glu Ile Arg Gln Ile
210 215 220
Glu Ala Phe Gly Thr Trp Pro Val Ala Thr Met Ser Ala Gly Glu Lys
225 230 235 240
Lys Val Val Val Glu Gly Pro Leu Cys Ser Asp Ser Lys Val Val Gly
245 250 255
Trp Phe Ser Arg Asp Phe Asn Asp Gly Thr Thr Arg Ala Val Thr Tyr
260 265 270
Asn Thr Glu Ala Asp Leu Asn Pro Ala Gly Ala Ala Gly Thr Arg Ala
275 280 285
His Thr Ile Asp Val Ser Gly Phe Ala Phe Asn Ser Ser Ile Leu Trp
290 295 300
Asp Pro Glu Arg Trp Gly Arg Pro Thr Ser Leu Pro Asp Thr Ser Gln
305 310 315 320
Asp Ser Ile Lys Phe Val Gln Glu Val Val Leu Glu Asp Arg Thr Lys
325 330 335
Leu Lys Gly Ile Pro Ser Asp Cys Ser Gln Ile Met Val Trp Gln Tyr
340 345 350
Thr Met Pro Met Gln Val His Ala Gln Thr Ser Thr Pro Lys Thr His
355 360 365
Asn Arg Arg
370
<210> 234
<211> 442
<212> PRT
<213> 毛果杨()
<220>
<223> 木聚糖生物合成酶不规则的木质部9 (IRX9) 同系物 GI:224092304
<400> 234
Met Ala Ser Ile Arg Arg Thr Leu Ser Pro Val Pro Arg Ala Gly Thr
1 5 10 15
Leu Leu Asn Gly Glu Ala Cys Gln Val Ala Ser Pro Leu Ser Lys Ser
20 25 30
Ser Ser Ser Tyr Ser Gln Ser Tyr Pro Thr Ser Gly Gly Phe Leu Ser
35 40 45
Ser Ile Phe Gly Leu Ser Asp Val Gln Ala Phe Ala Tyr Gly Val Phe
50 55 60
Ser Pro Arg Ser Ser Arg Pro Ser Glu Arg Ser Lys Ser Lys Gly Gln
65 70 75 80
Val Trp Lys Arg Ala Leu Phe His Phe Leu Val Ser Phe Val Ile Gly
85 90 95
Val Phe Ile Gly Leu Thr Pro Phe Val Ser Met Asn Leu Ser Thr Asn
100 105 110
Pro Met Ser Lys His Gln Ala Phe Ser Phe Glu Val Val Ser Thr Val
115 120 125
Gly Asn Phe Asp Lys His Glu Asp Met Thr Arg Asn Ala Thr Thr Ile
130 135 140
Ala Glu Arg Gly Gly Leu Glu Asn Ser Thr Thr Leu Glu Pro Gln Val
145 150 155 160
Lys Glu Glu Glu Ser Gly Asp Gly Asn Ser Asn Gly Thr Ser Ile Ser
165 170 175
Leu Ser Leu Ser Glu Asp Val Asn Leu Val Ser Arg Lys Leu Leu Ile
180 185 190
Ile Val Thr Pro Thr His Ala Arg Pro Leu Gln Ala Tyr Tyr Leu Ser
195 200 205
Arg Leu Ala His Thr Leu Lys Leu Val Gln Pro Pro Leu Leu Trp Ile
210 215 220
Val Val Glu Met Thr Leu Gln Ser Asp His Thr Ala Asp Ile Leu Arg
225 230 235 240
Arg Thr Gly Val Met Tyr Arg His Leu Val Cys Asn Lys Asn Leu Thr
245 250 255
Asp Ile Lys Asp Arg Ser Val His Gln Arg Asn Val Ala Leu Ser His
260 265 270
Ile Glu Ile His His Leu Asp Gly Ile Val His Phe Ala Asp Asp Tyr
275 280 285
Asn Thr Tyr Ser Ala Asp Leu Phe Glu Gln Met Arg Gln Ile Arg Arg
290 295 300
Phe Gly Thr Trp Thr Val Ala Lys Leu Thr Gly Asn Lys Asn Lys Asp
305 310 315 320
Phe Val Glu Gly Pro Ile Cys Asn Gly Thr Gln Val Ile Gly Trp His
325 330 335
Val Asn Asp Ser Arg Arg Arg Phe Arg Arg Phe His Ala Asp Met Ser
340 345 350
Gly Phe Ala Phe Asn Ser Thr Ile Ile Trp Asp Pro Lys Arg Trp His
355 360 365
Arg Pro Thr Pro Glu Pro Ile Arg Gln Leu Asp Thr Val Arg Asp Gly
370 375 380
Phe Gln Val Ser Ser Phe Ile Glu Gln Val Val Glu Asp Glu Ser Gln
385 390 395 400
Met Glu Gly Leu Leu Glu Asp Cys Ser Arg Val Met Val Trp Leu Leu
405 410 415
Gln Leu Gln Ser Ser Asn Ser Leu Tyr Pro Pro Lys Trp Phe Leu Asp
420 425 430
Val Asn Leu Asp Val Ile Thr Gln Ala Ala
435 440
<210> 235
<211> 357
<212> PRT
<213> 江南卷柏()
<220>
<223> 木聚糖生物合成酶不规则的木质部9 (IRX9) 同系物 GI:302759368
<400> 235
Met Ser Trp Met Lys Met Ala Ser Ala Arg Gln Gly Leu Asp Arg Ser
1 5 10 15
Lys Ala Arg Met Gln Ile Leu Arg Lys Pro Phe Phe His Phe Val Leu
20 25 30
Cys Phe Phe Val Gly Phe Gly Ile Gly Leu Thr Pro Leu Ser Ser Gly
35 40 45
Gly Gly Gly Ala Arg Glu Gln Gln Arg Pro Arg Glu Ser Phe Thr Ala
50 55 60
Ala Lys Arg Ile Ala Thr Glu Glu Gln Val Val Val Leu Asp Ser Ser
65 70 75 80
Ser Pro Leu Glu Ile Ser Ala Asn Ser Ser Arg His Pro Gly Glu Gly
85 90 95
Arg Met Pro Leu Gln Asp Pro Lys Lys Leu Leu Ile Ile Val Thr Pro
100 105 110
Thr Tyr Thr Arg Pro Phe Gln Ala Met Tyr Leu Thr Arg Leu Ala His
115 120 125
Thr Leu Lys Leu Val Asp Pro Pro Leu Leu Trp Ile Val Val Glu Met
130 135 140
Pro Gly Gln Ser Leu Glu Thr Ala Ser Leu Leu Arg Lys Thr Gly Val
145 150 155 160
Met Tyr Arg His Leu Ala Cys Glu Lys Asn Leu Thr Asn Val Lys Asp
165 170 175
Arg Gly Thr Gln Gln Arg Asn Leu Ala Leu Gln His Ile Glu Arg His
180 185 190
Gln Leu Asp Gly Ile Val Tyr Phe Ala Asp Asp Asp Asn Phe Tyr Ser
195 200 205
Leu Glu Leu Phe Asp Gln Leu Arg Glu Ile Lys Arg Phe Gly Thr Trp
210 215 220
Pro Val Ala Met Leu Ala His Ser Lys Ser Lys Thr Ile Leu Glu Gly
225 230 235 240
Pro Val Cys Asp Gly His Lys Val Thr Gly Trp His Thr Asn Glu Lys
245 250 255
Ser Lys Arg Leu Arg Arg Phe His Val Asp Met Ser Gly Phe Gly Phe
260 265 270
Asn Ser Thr Ile Leu Trp Asp Pro Arg Arg Trp Lys Arg Pro Thr Thr
275 280 285
Gln Pro Val Arg Gln Leu Asp Thr Val Lys Glu Gly Phe Gln Glu Thr
290 295 300
Thr Phe Ile Glu Gln Leu Val Glu Asp Glu Asn Gln Met Glu Gly Leu
305 310 315 320
Pro Ser Gly Cys Ser Lys Ile Met Val Trp His Leu His Leu Glu Ser
325 330 335
Gln Ile Ala Ser Tyr Pro Ser Asn Trp Lys Met Asp Lys Phe Leu Glu
340 345 350
Pro Val Ile His Leu
355
<210> 236
<211> 394
<212> PRT
<213> 拟南芥()
<220>
<223> 木聚糖生物合成酶不规则的木质部9-like (IRX9-like) GI:42571663
<400> 236
Met Ala Ser Ile Arg Arg Thr Leu Ser Pro Met Tyr His Asp Arg Ser
1 5 10 15
His Glu Asn Gly Gly Ser His Lys Gly Phe Thr Ile Gly Gly Ser Ser
20 25 30
Ser Lys His Asn Ser Ser Gln Phe Leu Ser Tyr Leu Thr Lys Leu Leu
35 40 45
Gly Val Thr Ser Asp Pro Lys Ser Ser Arg Arg Gly Pro Trp Arg Arg
50 55 60
Pro Phe Tyr Gln Phe Leu Val Phe Phe Leu Leu Gly Phe Val Leu Gly
65 70 75 80
Leu Thr Pro Phe Gly Lys Met Glu Asp Val Asn Gly Ser Asp Arg Phe
85 90 95
Ser Phe Glu Ile Lys Gln Pro Tyr Val Glu Glu Arg Leu Glu Asn Arg
100 105 110
Lys Arg Glu Glu Ala Ala Val Asp Ala Val Ser Phe Val Ala Glu Thr
115 120 125
Glu Asn Gly Lys Lys Glu Val Asn Phe Val Pro Lys Lys Leu Leu Ile
130 135 140
Val Val Thr Pro Thr Tyr Asn Arg Ala Met Gln Ala Tyr Tyr Leu Asn
145 150 155 160
Arg Val Ala Gln Thr Leu Arg Leu Val Glu Ser Pro Val Leu Trp Ile
165 170 175
Val Val Glu Gly Asn Val Ala Ser Phe Glu Thr Ser Glu Ile Leu Arg
180 185 190
Lys Thr Gly Val Met Tyr Arg His Leu Val Cys Lys Arg Asn Met Thr
195 200 205
Ser Ile Lys Asp Arg Gly Val His Gln Arg Asn Thr Ala Leu Glu His
210 215 220
Ile Glu Leu His Lys Leu Asp Gly Ile Val Tyr Phe Ala Asp Asp Asp
225 230 235 240
Asn Ile Tyr Ser Leu Glu Leu Phe Gln Ser Leu Arg Gln Ile Ser Arg
245 250 255
Phe Gly Thr Trp Pro Val Ala Met Leu Ala Gln Ser Lys Asn Lys Ala
260 265 270
Ile Leu Glu Gly Pro Val Cys Asn Gly Ser Gln Val Ile Gly Trp His
275 280 285
Thr Asn Glu Lys Ser Lys Arg Leu Arg Arg Phe His Val Asp Met Ser
290 295 300
Gly Phe Ala Phe Asn Ser Thr Ile Leu Trp Asp Pro Lys Arg Trp Arg
305 310 315 320
Arg Pro Phe Ser His Pro Thr Arg Gln Leu Asp Thr Val Lys Glu Gly
325 330 335
Phe Gln Glu Thr Ser Phe Ile Glu Gln Val Val Ala Asp Glu Ser Glu
340 345 350
Met Glu Gly Val Pro Pro Ala Cys Ser Ser Ile Leu Asn Trp His Leu
355 360 365
His Leu Asp Ala Leu Asp Val Pro Tyr Pro Gln Gly Trp Ala Ile Gln
370 375 380
Lys Asn Leu Gln Ala Leu Ile Thr Met Lys
385 390
<210> 237
<211> 395
<212> PRT
<213> 毛果杨()
<220>
<223> 木聚糖生物合成酶不规则的木质部9 (IRX9) 同系物 GI:224063335
<400> 237
Met Ala Ser Ile Arg Arg Thr Leu Ser Gln Val Tyr Gln Asp Arg Ser
1 5 10 15
Tyr Gln Asn Gly Val Ala Ser Ala Gln Ala His Lys Leu Phe Ser Thr
20 25 30
Asn Asn Asn Ser Gly Lys Tyr Ser Ser Leu Thr Pro Thr Ser Ala Val
35 40 45
Ala Ala Ala Ser Val Tyr Leu Arg Arg Lys Gly Phe Arg Arg Ser Phe
50 55 60
Tyr Arg Cys Thr Ile Phe Phe Ile Leu Gly Leu Leu Leu Gly Ile Phe
65 70 75 80
Pro Phe Gly Gln Val Asp Asn Asp Ile Asn Lys His Asp Phe Ser Phe
85 90 95
Glu Met Lys Pro Pro His Val Asn Val Gln Leu Asp Thr Lys Asp Asn
100 105 110
Phe Ala Leu Ala Ala Val Ser Leu Gly Val Glu Lys Thr Thr Pro Gln
115 120 125
Leu Asp Arg Phe Ser Arg Phe Asp Tyr Val Glu Arg Lys Gln Val Ile
130 135 140
Val Ile Thr Pro Thr Tyr Asn Arg Ala Leu Gln Ala Tyr Phe Leu Asn
145 150 155 160
Arg Leu Gly Gln Val Leu Arg Leu Val Gln Pro Pro Leu Leu Trp Ile
165 170 175
Val Val Glu Met Thr Ser Ala Ser Ala Glu Thr Ala Glu Ile Leu Arg
180 185 190
Lys Thr Gly Val Met Tyr Arg His Leu Val Cys Val Asn Lys Asn Asn
195 200 205
Thr Asn Val Lys Asp Arg Gly Val His Gln Arg Asn Ala Gly Leu Glu
210 215 220
His Ile Glu Arg His Arg Leu Asp Gly Ile Val Tyr Phe Ala Asp Asp
225 230 235 240
Asp Asn Val Tyr Ser Leu Gln Leu Phe Glu Ser Leu Arg Asn Ile Ser
245 250 255
His Phe Gly Thr Trp Pro Val Ala Met Leu Ala Gln Ser Lys Asn Lys
260 265 270
Ala Ile Val Glu Gly Pro Val Cys Asn Ala Ser Gln Val Ile Gly Trp
275 280 285
His Thr Asn Glu Lys Ser Lys Arg Leu Arg Arg Phe His Val Asp Met
290 295 300
Ser Gly Phe Ala Phe Asn Ser Thr Ile Leu Trp Asp Pro Lys Arg Trp
305 310 315 320
Asn Arg Pro Phe Ser Asn Pro Ile Arg Gln Leu Asp Thr Val Lys Glu
325 330 335
Gly Phe Gln Glu Thr Thr Phe Ile Glu Gln Val Val Glu Asp Glu Ser
340 345 350
Gln Met Glu Ser Val Pro Pro Ser Cys Ser Arg Ile Leu Asn Trp His
355 360 365
Leu His Leu Asp Ala His Gly Leu Val Tyr Pro Arg Gly Trp Leu Leu
370 375 380
Gln Lys Asn Leu Glu Val Val Gln Pro Ile Lys
385 390 395
<210> 238
<211> 446
<212> PRT
<213> 水稻()
<220>
<223> 木聚糖生物合成酶不规则的木质部9 (IRX9) 同系物 GI:115439133
<400> 238
Met Ser Arg Arg Asn Ala Gly Ala Met Gln Arg Glu Gly Ser Val Lys
1 5 10 15
Asp Trp Glu Glu Phe Asp Pro Ser Pro Ser Pro Lys Leu Ala Tyr Ser
20 25 30
Gln Ser Tyr Val Ala Met Arg Gly Leu Leu Thr Ser Val Ala Ser Leu
35 40 45
Asp Leu Val Leu Met Ser Ser Ser Leu Lys Ser Ala Trp Ala Ala Ile
50 55 60
Ser Ser His Lys His Ala Arg Ser Leu Glu Arg Ser Arg Ser Lys Gly
65 70 75 80
Met Ser Leu Lys Arg Ala Met Leu Gln Leu Leu Val Cys Phe Met Val
85 90 95
Gly Ile Phe Ile Gly Phe Thr Pro Pro Phe Ser Val Asp Leu Pro Gly
100 105 110
Lys Ile Ala Ser Glu Asn Gly Arg Leu Pro Phe Asp Gly Asp Ala Ile
115 120 125
Asp Arg Arg Gln Met Val Glu Arg Gln Gly Thr Lys Leu Glu Pro Phe
130 135 140
Val Ala Glu Ala Glu Ser Glu Ala Ser Ser Glu Pro Gln Val Glu Glu
145 150 155 160
Gly Pro Pro Val Pro Ala Met Leu Asp Asp Glu Ala Asp Phe Val Glu
165 170 175
Ala Ser Pro Ile Val His Ser Val Asn Asp Ser Gly Ile Val Val Arg
180 185 190
Lys His Leu Ile Ile Ile Thr Thr Thr Ser Val Arg Pro His Gln Ala
195 200 205
Tyr Tyr Leu Asn Arg Leu Ala His Val Leu Lys Asp Val Pro Pro Pro
210 215 220
Leu Leu Trp Ile Val Ala Glu Trp Pro Tyr Gln Ser Arg Glu Thr Ala
225 230 235 240
Glu Ile Leu Arg Ser Ser Gly Ile Met Tyr Arg His Leu Ile Cys Asn
245 250 255
Arg Asn Thr Thr Asn Ile Arg Lys Ile Val Val Cys Gln Lys Asn Asn
260 265 270
Ala Ile Phe His Ile Lys Lys His Arg Leu Asp Gly Ile Val His Phe
275 280 285
Ala Asp Glu Glu Arg Ala Tyr Ser Ala Asp Leu Phe Glu Glu Met Arg
290 295 300
Lys Ile Arg Arg Phe Gly Thr Trp Pro Val Ala Ile His Val Gly Thr
305 310 315 320
Lys Tyr Arg Val Val Leu Glu Gly Pro Val Cys Lys Gly Asn Gln Val
325 330 335
Thr Gly Trp His Thr Asn Gln Arg Arg Gly Val Ser Arg Arg Phe Pro
340 345 350
Ile Gly Phe Ser Gly Phe Ala Phe Asn Ser Thr Ile Leu Trp Asp Pro
355 360 365
Gln Arg Trp Asn Ser Pro Thr Leu Glu Ser Ile Ile Val His Ser Gly
370 375 380
Gly Arg Gly Gly Leu Gln Glu Ser Arg Phe Ile Glu Lys Leu Val Glu
385 390 395 400
Asp Glu Ser Gln Met Glu Gly Leu Gly Asp Asn Cys Thr Arg Val Met
405 410 415
Val Trp Asn Phe Glu Leu Glu Pro Pro Gln Val Asn Tyr Pro Ile Gly
420 425 430
Trp Leu Leu Gln Arg Asn Leu Asp Ala Val Val Pro Ile Thr
435 440 445
<210> 239
<211> 338
<212> PRT
<213> 水稻()
<220>
<223> 木聚糖生物合成酶不规则的木质部9 (IRX9) 同系物 GI:115474279
<400> 239
Met Ala Ser Ala Gly Gly Cys Lys Lys Lys Thr Gly Asn Ser Arg Ser
1 5 10 15
Arg Ser Pro Arg Ser Pro Val Val Leu Arg Arg Ala Met Leu His Ser
20 25 30
Ser Leu Cys Phe Leu Val Gly Leu Leu Ala Gly Leu Ala Ala Pro Ser
35 40 45
Asp Trp Pro Ala Ala Ala Gly Ala Ala Val Phe Leu Arg Thr Leu Arg
50 55 60
Ala Ser Asn Val Ile Phe Ser Arg Ser Ser Asn Arg Pro Gln Gln Pro
65 70 75 80
Gln Leu Val Val Val Val Thr Thr Thr Glu Gln Ser Asp Asp Ser Glu
85 90 95
Arg Arg Ala Ala Gly Leu Thr Arg Thr Ala His Ala Leu Arg Leu Val
100 105 110
Ser Pro Pro Leu Leu Trp Leu Val Val Glu Glu Ala Pro Ala Glu Lys
115 120 125
His Ala Ala Pro Pro Thr Ala Arg Leu Leu Arg Arg Thr Gly Val Val
130 135 140
His Arg His Leu Leu Met Lys Gln Gly Asp Asp Asp Phe Ser Met Gln
145 150 155 160
Ile Ser Met Arg Arg Glu Gln Gln Arg Asn Val Ala Leu Arg His Ile
165 170 175
Glu Asp His Arg Ile Ala Gly Val Val Leu Phe Gly Gly Leu Ala Asp
180 185 190
Ile Tyr Asp Leu Arg Leu Leu His His Leu Arg Asp Ile Arg Thr Phe
195 200 205
Gly Ala Trp Pro Val Ala Thr Val Ser Ala Tyr Glu Arg Lys Val Met
210 215 220
Val Gln Gly Pro Leu Cys Ile Asn Thr Ser Ser Ser Ser Val Ile Thr
225 230 235 240
Arg Gly Trp Phe Asp Met Asp Met Asp Met Ala Ala Gly Gly Glu Arg
245 250 255
Arg Ala Ala Ala Asp Arg Pro Pro Pro Glu Thr Leu Met Glu Val Gly
260 265 270
Gly Phe Ala Phe Ser Ser Trp Met Leu Trp Asp Pro His Arg Trp Asp
275 280 285
Arg Phe Pro Leu Ser Asp Pro Asp Ala Ser Gln Glu Ser Val Lys Phe
290 295 300
Val Gln Arg Val Ala Val Glu Glu Tyr Asn Gln Ser Thr Thr Arg Gly
305 310 315 320
Met Pro Asp Ser Asp Cys Ser Gln Ile Met Leu Trp Arg Ile Gln Thr
325 330 335
Thr Leu
<210> 240
<211> 451
<212> PRT
<213> 水稻()
<220>
<223> 木聚糖生物合成酶不规则的木质部9 (IRX9) 同系物 GI:115465403
<400> 240
Met Val Ser Ser Arg Arg Asn Thr Gly Gly Ile Gln Arg Asp Gly Ser
1 5 10 15
Leu Arg Asp Trp Ser Glu Phe Val Asp Pro Ser Pro Ser Pro Lys Leu
20 25 30
Leu Tyr Ser Gln Ser Tyr Val Ala Met Arg Gly Leu Leu Ser Ser Leu
35 40 45
Val Ser Met Asp Phe Ala Leu Leu Ser Ser Arg Leu Lys Ser Ala Trp
50 55 60
Ala Ala Ile Leu Ser Gln Arg His Thr Arg Ser Pro Glu Arg Ser Lys
65 70 75 80
Ser Arg Gly Leu Ser Cys Lys Arg Leu Ala Phe His Leu Phe Val Cys
85 90 95
Phe Met Val Gly Ile Phe Ile Gly Phe Met Pro Phe Phe Ser Val Asp
100 105 110
Val Ser Gln Lys Ile Val Ser Glu Asn Gly Arg Leu Pro Phe Asp Glu
115 120 125
Gly Ala Val Asp Arg Gly Met Val Asp Gly Lys Val Lys Glu Leu Glu
130 135 140
Thr Ile Val Val Glu Lys Glu Val Asp Ile Ile Asp Glu Ser Glu Val
145 150 155 160
Glu Glu Ser Pro Pro Val Pro Ala Met Leu Asp Asp Glu Ala Asp Phe
165 170 175
Val Glu Ser Ala Pro Ala Ile Pro Asp Ile Asn Asp Leu Asp Ile Thr
180 185 190
Val Arg Lys Leu Leu Ile Ile Val Thr Ile Thr Thr Val Arg Pro Gln
195 200 205
Gln Ala Tyr Tyr Leu Asn Arg Leu Ala His Val Leu Lys Thr Val Gln
210 215 220
Ser Pro Leu Leu Trp Leu Val Val Glu Trp Pro Asp Gln Ser Phe Gln
225 230 235 240
Thr Ala Glu Ile Leu Arg Ser Ser Gly Val Met Tyr Arg His Leu Ile
245 250 255
Cys Arg Lys Asn Thr Thr Ser Val Arg Lys Ile Ala Val Cys Gln Arg
260 265 270
Asn Thr Ala Ile Tyr His Ile Lys Lys His Arg Leu Asp Gly Ile Met
275 280 285
His Phe Ala Asp Glu Glu Arg Ser Tyr Met Ser Asp Val Phe Glu Glu
290 295 300
Met Arg Lys Ile Arg Arg Phe Gly Ala Trp Pro Val Ala Ile His Thr
305 310 315 320
Gly Ile Lys Tyr Arg Val Val Leu Glu Gly Pro Ile Cys Lys Gly Asn
325 330 335
Arg Val Thr Gly Trp Asn Thr Ile Gln Asn Ile Gln Lys Lys Ser Ala
340 345 350
Val Arg Arg Phe Pro Val Gly Phe Ser Gly Phe Ala Phe Asn Ser Thr
355 360 365
Met Leu Trp Asp Pro Glu Arg Trp Asn Arg Pro Pro Met Asp Ser Val
370 375 380
Ile Val His Ser Gly Gly Arg Gly Gly Leu Gln Glu Ser Arg Phe Ile
385 390 395 400
Glu Lys Leu Val Lys His Glu Arg Gln Ile Glu Gly Leu Pro Glu Asp
405 410 415
Cys Asn Arg Val Met Val Trp Asn Phe Asn Leu Glu Pro Pro Leu Leu
420 425 430
Asn Val Pro Pro Gly Trp Ser Leu His Lys Asn Leu Asp Ala Val Ile
435 440 445
Pro Val Thr
450
<210> 241
<211> 351
<212> PRT
<213> 水稻()
<220>
<223> 木聚糖生物合成酶不规则的木质部9 (IRX9) 同系物 GI:115481434
<400> 241
Met Ala Ala Pro Pro Cys Pro Pro Arg Arg Pro Ile Ser Ala Pro Cys
1 5 10 15
Phe Leu Leu Cys Phe Leu Leu Gly Phe Val Ala Gly Leu Phe Pro Phe
20 25 30
Ala His Arg His Leu His Leu Asp Leu His Leu Pro Leu Pro Pro Pro
35 40 45
Ala Thr Ala Ile Leu Val Arg Glu Asp Pro Pro Ser Val Val Val Asp
50 55 60
Val Asp Thr Pro Leu Pro Ala Ala Ala Glu Glu Arg Lys Leu Leu Leu
65 70 75 80
Val Val Thr Pro Thr Arg Ala Arg Pro Leu Gln Ala Tyr Tyr Leu Arg
85 90 95
Arg Leu Ala His Thr Leu Arg Leu Ala Pro Ser Pro Leu Leu Trp Leu
100 105 110
Val Val Glu Ser Gly Ala Ala Thr Arg Asp Thr Ala Ala Leu Leu Arg
115 120 125
Gly Cys Gly Val Met Tyr Arg His Leu Ser Ser Pro Val Pro Asp Ala
130 135 140
Pro Gln Asp Arg Pro Arg Arg Arg Gly Arg Arg Gln Asp Arg Pro Ala
145 150 155 160
Val Asp Ser Arg Ala Arg Gln Arg Asn Thr Ala Leu Asp His Ile Glu
165 170 175
His His Arg Leu His Gly Ile Val Tyr Phe Ala Asp Glu Asp Asn Val
180 185 190
Tyr Ser Leu Asp Leu Phe Tyr His Leu Arg Asp Ile Arg Ser Phe Gly
195 200 205
Thr Trp Pro Val Ala Thr Leu Ala Pro Gly Lys Ser Lys Thr Ile Leu
210 215 220
Gln Gly Pro Val Cys Glu Gly Ser Arg Val Val Gly Trp His Thr Thr
225 230 235 240
Asp Arg Ser Lys Asn Gln Arg Arg Phe His Val Asp Met Ser Gly Phe
245 250 255
Ala Phe Asn Ser Ser Lys Leu Trp Asp Ala Lys Asn Arg Gly His Gln
260 265 270
Ala Trp Asn Tyr Ile Arg Gln Leu Asp Thr Ala Lys Glu Gly Phe Gln
275 280 285
Glu Thr Ala Phe Ile Glu Gln Leu Val Glu Asp Glu Thr His Met Glu
290 295 300
Gly Val Pro Pro Gly Cys Ser Lys Ile Met Asn Phe His Leu His Leu
305 310 315 320
Glu Asp Lys Asn Ala Ile Tyr Leu Asn Gly Trp Gln Thr Thr Gln Asn
325 330 335
Leu Asp Val Ile Ile Pro Leu Lys Lys Glu Ala Arg Pro Leu Leu
340 345 350
<210> 242
<211> 381
<212> PRT
<213> 水稻()
<220>
<223> 木聚糖生物合成酶不规则的木质部9 (IRX9) 同系物 GI:115456794
<400> 242
Met Ala Ser Ile Arg Arg Pro His Ser Pro Ala Lys Gln Gln His Leu
1 5 10 15
Leu Arg His Gly His Leu Gly Pro Phe Ala Ser Ser Ser Pro Pro Ser
20 25 30
Ser Pro Leu Arg His Ser Ser Ser Ser Ser Ser Pro Arg Ser Ala Ala
35 40 45
His His His His His Leu Leu Ala Ala Ala Gly His Thr Ser Phe Arg
50 55 60
Arg Pro Leu Pro Arg Phe Ala Ala Phe Phe Leu Leu Gly Ser Phe Leu
65 70 75 80
Gly Leu Leu His Phe Leu Ser His Leu Pro Arg Pro Leu Gly Pro Ile
85 90 95
Pro Asn Pro Asn Ser His His Arg His Arg Asp Pro Phe Pro Ile Leu
100 105 110
Gln His Pro His Pro Pro Ser Thr Pro His Ser Asn His Lys Leu Leu
115 120 125
Ile Val Val Thr Pro Thr Arg Ala Arg Pro Ser Gln Ala Tyr Tyr Leu
130 135 140
Thr Arg Met Ala His Thr Leu Arg Leu Leu His Asp Ser Pro Leu Leu
145 150 155 160
Trp Ile Val Val Gln Ala Gly Asn Pro Thr Pro Glu Ala Ala Ala Ala
165 170 175
Leu Arg Arg Thr Ala Val Leu His Arg Tyr Val Gly Cys Cys His Asn
180 185 190
Ile Asn Ala Ser Ala Pro Asp Phe Arg Pro His Gln Ile Asn Ala Ala
195 200 205
Leu Asp Ile Val Asp Asn His Arg Leu Asp Gly Val Leu Tyr Phe Ala
210 215 220
Asp Glu Glu Gly Val Tyr Ser Leu His Leu Phe His His Leu Arg Gln
225 230 235 240
Ile Arg Arg Phe Ala Thr Trp Pro Val Pro Glu Ile Ser Gln His Thr
245 250 255
Asn Glu Val Val Leu Gln Gly Pro Val Cys Lys Gln Gly Gln Val Val
260 265 270
Gly Trp His Thr Thr His Asp Gly Asn Lys Leu Arg Arg Phe His Leu
275 280 285
Ala Met Ser Gly Phe Ala Phe Asn Ser Thr Met Leu Trp Asp Pro Lys
290 295 300
Leu Arg Ser His Leu Ala Trp Asn Ser Ile Arg His Pro Glu Met Val
305 310 315 320
Lys Glu Ser Leu Gln Gly Ser Ala Phe Val Glu Gln Leu Val Glu Asp
325 330 335
Glu Ser Gln Met Glu Gly Ile Pro Ala Asp Cys Ser Gln Ile Met Asn
340 345 350
Trp His Val Pro Phe Gly Ser Glu Ser Val Val Tyr Pro Lys Gly Trp
355 360 365
Arg Val Ala Thr Asp Leu Asp Val Ile Ile Pro Leu Lys
370 375 380
<210> 243
<211> 448
<212> PRT
<213> 拟南芥()
<220>
<223> 木聚糖生物合成酶不规则的木质部7 (IRX7, FRA8) GI:42570324
<400> 243
Met Thr Thr His Lys His Arg Arg Thr Glu Lys Asn Leu Cys Phe Lys
1 5 10 15
Gln Tyr Tyr Lys Trp Ile Leu Cys Phe Ile Leu Thr Leu Tyr Phe Phe
20 25 30
Ala Ser Phe Phe Val Asp His Asp Gln Asp His Arg Ser Ser Thr Ser
35 40 45
Ile Ser Lys His Leu Leu Thr Asn His Lys Pro Lys Leu Phe Ala Ser
50 55 60
Arg Ala Met Phe Glu Ser Lys Ile His Asp His Lys Leu Gly Phe Thr
65 70 75 80
Ser Gln Gln Pro Asn Ile Lys Thr Asp Val Phe Asn Asn Leu Lys Ile
85 90 95
Tyr Val Tyr Asp Leu Pro Ser Lys Phe Asn Lys Asp Trp Leu Ala Asn
100 105 110
Asp Arg Cys Thr Asn His Leu Phe Ala Ala Glu Val Ala Leu His Lys
115 120 125
Ala Phe Leu Ser Leu Glu Gly Asp Val Arg Thr Glu Asp Pro Tyr Glu
130 135 140
Ala Asp Phe Phe Phe Val Pro Val Tyr Val Ser Cys Asn Phe Ser Thr
145 150 155 160
Ile Asn Gly Phe Pro Ala Ile Gly His Ala Arg Ser Leu Ile Asn Asp
165 170 175
Ala Ile Lys Leu Val Ser Thr Gln Tyr Pro Phe Trp Asn Arg Thr Ser
180 185 190
Gly Ser Asp His Val Phe Thr Ala Thr His Asp Phe Gly Ser Cys Phe
195 200 205
His Thr Met Glu Asp Arg Ala Ile Ala Asp Gly Val Pro Ile Phe Leu
210 215 220
Arg Asn Ser Ile Ile Leu Gln Thr Phe Gly Val Thr Phe Asn His Pro
225 230 235 240
Cys Gln Glu Val Glu Asn Val Val Ile Pro Pro Tyr Ile Ser Pro Glu
245 250 255
Ser Leu His Lys Thr Gln Lys Asn Ile Pro Val Thr Lys Glu Arg Asp
260 265 270
Ile Trp Val Phe Phe Arg Gly Lys Met Glu Leu His Pro Lys Asn Ile
275 280 285
Ser Gly Arg Phe Tyr Ser Lys Arg Val Arg Thr Asn Ile Trp Arg Ser
290 295 300
Tyr Gly Gly Asp Arg Arg Phe Tyr Leu Gln Arg Gln Arg Phe Ala Gly
305 310 315 320
Tyr Gln Ser Glu Ile Ala Arg Ser Val Phe Cys Leu Cys Pro Leu Gly
325 330 335
Trp Ala Pro Trp Ser Pro Arg Leu Val Glu Ser Val Ala Leu Gly Cys
340 345 350
Val Pro Val Ile Ile Ala Asp Gly Ile Arg Leu Pro Phe Pro Ser Thr
355 360 365
Val Arg Trp Pro Asp Ile Ser Leu Thr Val Ala Glu Arg Asp Val Gly
370 375 380
Lys Leu Gly Asp Ile Leu Glu His Val Ala Ala Thr Asn Leu Ser Val
385 390 395 400
Ile Gln Arg Asn Leu Glu Asp Pro Ser Val Arg Arg Ala Leu Met Phe
405 410 415
Asn Val Pro Ser Arg Glu Gly Asp Ala Thr Trp Gln Val Leu Glu Ala
420 425 430
Leu Ser Lys Lys Leu Asn Arg Ser Val Arg Arg Ser Asn Ser Phe Leu
435 440 445
<210> 244
<211> 462
<212> PRT
<213> 毛果杨()
<220>
<223> 木聚糖生物合成酶不规则的木质部7 (IRX7) 同系物 GI:224106838
<400> 244
Met Val Leu Glu Val Lys Arg Pro Thr Met Ser Asn Asn Arg Arg Gly
1 5 10 15
Phe Tyr Val Arg Met Lys Leu Leu His Arg His Ala Gly Leu Gln Gln
20 25 30
Gln Glu Lys Lys Lys Asn Leu Cys Phe Lys Tyr Tyr Lys Trp Leu Leu
35 40 45
Trp Phe Ser Leu Leu Leu Tyr Phe Leu Ser Ser Cys Phe Phe Thr His
50 55 60
Lys Pro Ile Pro Leu Ser Lys Thr His Val Ser Glu Ser Lys Thr Val
65 70 75 80
Val Ser Arg Ala Leu Phe Glu Ser Ser Asn Ser Thr Phe Ile Gln Gln
85 90 95
Ser Lys Asn Ile Asn Arg Gly Leu Leu Lys Asp Leu Lys Val Tyr Ile
100 105 110
Tyr Glu Leu Pro Ser Lys Tyr Asn Thr Asp Trp Leu Ala Asn Glu Arg
115 120 125
Cys Ser Asn His Leu Phe Ala Ser Glu Val Ala Ile His Lys Ala Leu
130 135 140
Ser Asn Ser Leu Asp Ile Arg Thr Phe Asp Pro Tyr Glu Ala Asp Phe
145 150 155 160
Phe Phe Val Pro Val Tyr Val Ser Cys Asn Phe Ser Thr Val Asn Gly
165 170 175
Phe Pro Ala Ile Gly His Ala Arg Ser Leu Leu Ser Ser Ala Val Gln
180 185 190
Leu Ile Ser Ser Asn Tyr Pro Phe Trp Asn Arg Ser Gln Gly Ser Asp
195 200 205
His Val Phe Val Ala Ser His Asp Tyr Gly Ala Cys Phe His Ala Met
210 215 220
Glu Glu Arg Ala Met Glu Asp Gly Ile Pro Glu Phe Leu Lys Arg Ser
225 230 235 240
Ile Ile Leu Gln Thr Phe Gly Val Lys Phe Asn His Pro Cys Gln Asp
245 250 255
Val Glu Asn Val Val Ile Pro Pro Tyr Ile Ser Pro Glu Arg Val Arg
260 265 270
Thr Thr Leu Glu Asn Tyr Pro Leu Asn Gly Arg Arg Asp Ile Trp Ala
275 280 285
Phe Phe Arg Gly Lys Met Glu Val His Pro Lys Asn Ile Ser Gly Arg
290 295 300
Tyr Tyr Ser Lys Lys Val Arg Thr Val Ile Trp Arg Lys Tyr Ser Gly
305 310 315 320
Asp Arg Arg Phe Tyr Leu Gln Arg His Arg Phe Ala Gly Tyr Gln Ser
325 330 335
Glu Ile Val Arg Ser Val Phe Cys Leu Cys Pro Leu Gly Trp Ala Pro
340 345 350
Trp Ser Pro Arg Leu Val Glu Ser Val Ala Leu Gly Cys Val Pro Val
355 360 365
Ile Ile Ala Asp Gly Ile Arg Leu Pro Phe Pro Thr Ala Val Arg Trp
370 375 380
Ser Glu Ile Ser Leu Thr Val Ala Glu Lys Asp Val Ala Asn Leu Gly
385 390 395 400
Thr Leu Leu Asp His Val Ala Ala Thr Asn Leu Ser Ala Ile Gln Lys
405 410 415
Asn Leu Trp Asp Pro Asp Val Arg Arg Ala Leu Leu Phe Asn Asp Arg
420 425 430
Val Gln Glu Gly Asp Ala Thr Trp Gln Val Leu Tyr Ala Leu Ala Arg
435 440 445
Lys Leu Asp Arg Ser Tyr Arg Thr Val Arg Leu Pro Asn Gln
450 455 460
<210> 245
<211> 469
<212> PRT
<213> 拟南芥()
<220>
<223> 木聚糖生物合成酶不规则的木质部7-like (IRX7-like, F8H) GI:42568020
<400> 245
Met Ser Leu Asp Ile Lys Lys Pro Asn Ile Thr Lys Thr Lys Lys Lys
1 5 10 15
Lys Thr Gly Phe Val Val Lys Met Gln Leu Asn Asn Asn Arg Gly Gly
20 25 30
Asn Lys Arg Asn Ile Phe Ile Phe Phe Phe Phe Arg Asn Tyr Tyr Thr
35 40 45
Trp Ile Leu Trp Phe Cys Leu Ser Leu Tyr Phe Phe Thr Ser Tyr Phe
50 55 60
Ser Val Glu Asp Gln Ser Ser Pro Ser Ser Ile Arg Leu Leu Ser Asn
65 70 75 80
His Lys Thr Ser Ser Ser Leu Pro Ser Arg Ala Leu Ile Glu Ser Ser
85 90 95
Ala Ile Lys Thr Thr Ser Ile Gly Leu Phe Thr Gly Met Lys Ile Tyr
100 105 110
Val Tyr Asp Leu Pro Ala Ser Tyr Asn Asp Asp Trp Val Thr Ala Ser
115 120 125
Asp Arg Cys Ala Ser His Leu Phe Ala Ala Glu Val Ala Ile His Arg
130 135 140
Ala Leu Leu Ser Ser Asp Val Arg Thr Leu Asp Pro Asp Glu Ala Asp
145 150 155 160
Tyr Phe Phe Val Pro Val Tyr Val Ser Cys Asn Phe Ser Thr Ser Asn
165 170 175
Gly Phe Pro Ser Leu Ser His Ala Arg Ser Leu Leu Ser Ser Ala Val
180 185 190
Asp Phe Leu Ser Asp His Tyr Pro Phe Trp Asn Arg Ser Gln Gly Ser
195 200 205
Asp His Val Phe Val Ala Ser His Asp Phe Gly Ala Cys Phe His Ala
210 215 220
Met Glu Asp Met Ala Ile Glu Glu Gly Ile Pro Lys Phe Met Lys Arg
225 230 235 240
Ser Ile Ile Leu Gln Thr Phe Gly Val Lys Tyr Lys His Pro Cys Gln
245 250 255
Glu Val Glu His Val Val Ile Pro Pro Tyr Ile Pro Pro Glu Ser Val
260 265 270
Gln Lys Ala Ile Glu Lys Ala Pro Val Asn Gly Arg Arg Asp Ile Trp
275 280 285
Ala Phe Phe Arg Gly Lys Met Glu Val Asn Pro Lys Asn Ile Ser Gly
290 295 300
Arg Phe Tyr Ser Lys Gly Val Arg Thr Ala Ile Leu Lys Lys Phe Gly
305 310 315 320
Gly Arg Arg Arg Phe Tyr Leu Asn Arg His Arg Phe Ala Gly Tyr Arg
325 330 335
Ser Glu Ile Val Arg Ser Val Phe Cys Leu Cys Pro Leu Gly Trp Ala
340 345 350
Pro Trp Ser Pro Arg Leu Val Glu Ser Ala Val Leu Gly Cys Val Pro
355 360 365
Val Val Ile Ala Asp Gly Ile Gln Leu Pro Phe Ser Glu Thr Val Gln
370 375 380
Trp Pro Glu Ile Ser Leu Thr Val Ala Glu Lys Asp Val Arg Asn Leu
385 390 395 400
Arg Lys Val Leu Glu His Val Ala Ala Thr Asn Leu Ser Ala Ile Gln
405 410 415
Arg Asn Leu His Glu Pro Val Phe Lys Arg Ala Leu Leu Tyr Asn Val
420 425 430
Pro Met Lys Glu Gly Asp Ala Thr Trp His Ile Leu Glu Ser Leu Trp
435 440 445
Arg Lys Leu Asp Asp Arg Ser Tyr Arg Arg Ser Arg Val Leu Ser Gln
450 455 460
Arg Glu Val Asp Met
465
<210> 246
<211> 427
<212> PRT
<213> 水稻()
<220>
<223> 木聚糖生物合成酶不规则的木质部7 (IRX7) 同系物 GI:115450193
<400> 246
Met Ala Met Arg Gly Asp Pro Lys Gln Arg Arg Ala Ser Ala Ser Ala
1 5 10 15
Pro His Gly Gly Ala Ala His His Val Ala Asp Lys Leu Arg Arg His
20 25 30
Ser Thr Phe Leu Leu Leu Leu Leu Leu Leu Trp Phe Ala Leu Ser Leu
35 40 45
Tyr Leu Phe Leu Ser Ala Thr Pro Pro Pro Pro Arg Pro Ala Phe Leu
50 55 60
Pro Ser Thr Ser Thr Pro Arg Pro Ala Leu Arg Ile Tyr Val Tyr Asp
65 70 75 80
Leu Pro Ala Arg Phe Asn Arg His Trp Val Ala Ala Asp Ala Arg Cys
85 90 95
Ala Thr His Leu Phe Ala Ala Glu Val Ala Leu His Glu Ala Leu Leu
100 105 110
Ala Tyr Ala Gly Arg Ala Ala Arg Pro Asp Asp Ala Thr Leu Phe Phe
115 120 125
Val Pro Val Tyr Val Ser Cys Asn Phe Ser Thr Asp Asn Gly Phe Pro
130 135 140
Ser Leu Ser His Ala Arg Ala Leu Leu Ala Asp Ala Val Asp Leu Val
145 150 155 160
Arg Ala Gln Met Pro Tyr Trp Asn Arg Ser Ala Gly Ala Asp His Val
165 170 175
Phe Val Ala Ser His Asp Phe Gly Ala Cys Phe His Pro Met Glu Asp
180 185 190
Val Ala Ile Ala Asp Gly Ile Pro Glu Phe Leu Lys Arg Ser Ile Leu
195 200 205
Leu Gln Thr Phe Gly Val Gln Gly Thr His Val Cys Gln Glu Ala Asp
210 215 220
His Val Val Ile Pro Pro His Val Pro Pro Glu Val Ala Leu Glu Leu
225 230 235 240
Pro Glu Pro Glu Lys Ala Gln Arg Asp Ile Phe Ala Phe Phe Arg Gly
245 250 255
Lys Met Glu Val His Pro Lys Asn Ile Ser Gly Arg Phe Tyr Ser Lys
260 265 270
Lys Val Arg Thr Glu Leu Leu Gln Lys Tyr Gly Arg Asn Arg Lys Phe
275 280 285
Tyr Leu Lys Arg Lys Arg Tyr Gly Asn Tyr Arg Ser Glu Met Ala Arg
290 295 300
Ser Leu Phe Cys Leu Cys Pro Leu Gly Trp Ala Pro Trp Ser Pro Arg
305 310 315 320
Leu Val Glu Ser Val Leu Leu Gly Cys Ile Pro Val Ile Ile Ala Asp
325 330 335
Asp Ile Arg Leu Pro Phe Pro Ser Val Leu Gln Trp Leu Asp Ile Ser
340 345 350
Leu Gln Val Ala Glu Lys Asp Val Ala Ser Leu Glu Met Val Leu Asp
355 360 365
His Val Val Ala Thr Asn Leu Thr Val Ile Gln Lys Asn Leu Trp Asp
370 375 380
Pro Val Lys Arg Lys Ala Leu Val Phe Asn Arg Pro Met Glu Glu Gly
385 390 395 400
Asp Ala Thr Trp Gln Val Leu Arg Glu Leu Glu Ile Leu Leu Asp Arg
405 410 415
Ser Gln Arg Arg His Val Glu Ser Trp Lys Arg
420 425
<210> 247
<211> 405
<212> PRT
<213> 江南卷柏()
<220>
<223> 木聚糖生物合成酶不规则的木质部7 (IRX7) 同系物 GI:302786830
<400> 247
Met Glu Gly Ser Ser Asn Ile Leu Ala Gly Ala Leu Leu Thr Ile Met
1 5 10 15
Thr Arg Thr Val Phe Ile Ala Arg Glu Pro Leu Ala Gln Val Gln Thr
20 25 30
Asn Lys Cys Gly Pro His Tyr Phe Val Arg Asn Gly Cys Gly Gly Pro
35 40 45
Leu Gly Leu Ser Ser Asn Ile Lys Val Tyr Ile Tyr Asp Leu Pro Ser
50 55 60
Ser Tyr Asn Thr Asp Trp Leu Val Asp Ser Arg Cys Ser Ser His Leu
65 70 75 80
Phe Ala Ala Glu Val Ala Ile His Gln Asn Leu Leu Arg Ser Pro Val
85 90 95
Arg Thr Leu Asp Pro Asp Glu Ala Asp Phe Phe Phe Met Pro Val Tyr
100 105 110
Val Ser Cys Asn Phe Thr Ser Arg Ser Gly Phe Pro Thr Leu Phe His
115 120 125
Ala Ser Asp Ile Leu Gln Ala Ala Val Gly Leu Val Ser Arg Asn Met
130 135 140
Pro Phe Trp Asp Arg His Gln Gly Arg Asp His Val Phe Val Ala Thr
145 150 155 160
His Asp Phe Gly Ala Cys Phe His Ala Met Glu Asp Leu Ala Val Thr
165 170 175
Met Gly Ile Pro Gln Phe Leu Arg Asn Ser Ile Ile Leu Gln Thr Phe
180 185 190
Gly Glu Lys Asn Lys His Pro Cys Gln Asn Val Asp His Ile Gln Ile
195 200 205
Pro Pro Tyr Val Val Pro Ala Lys Lys Leu Pro Asp Pro Arg Ser Gln
210 215 220
Arg Arg Lys Ile Leu Ala Phe Phe Arg Gly Lys Met Glu Ile His Pro
225 230 235 240
Lys Asn Val Ser Gly His Met Tyr Ser Arg Gly Val Arg Thr Thr Ile
245 250 255
Trp Arg Arg Phe Ser His Asp Arg Arg Phe Phe Ile Lys Arg Lys Arg
260 265 270
Ser Asp Asn Tyr Lys Ala Glu Met Leu Arg Ser Val Phe Cys Leu Cys
275 280 285
Pro Leu Gly Trp Ala Pro Trp Ser Pro Arg Ile Val Glu Ser Val Ile
290 295 300
Gln Gly Cys Ile Pro Val Ile Ile Ala Asp Asn Ile Gln Leu Pro Tyr
305 310 315 320
Ser His Val Ile Asp Trp Arg Lys Ile Ser Val Thr Val Ala Glu Arg
325 330 335
Asp Val His Lys Leu Asp Arg Ile Leu Ser Arg Val Ala Ala Thr Asn
340 345 350
Val Ser Met Ile Gln Ala Asn Leu Trp Arg Asp Glu Val Arg Gln Ala
355 360 365
Leu Val Tyr Asn Gln Pro Leu Val Arg Gly Asp Ala Thr Trp Gln Val
370 375 380
Leu Asp Leu Leu Ser Lys Arg Lys Asn Lys Val Ala Glu Ala Gly Ser
385 390 395 400
Arg Ile Lys Val Ala
405
<210> 248
<211> 332
<212> PRT
<213> 江南卷柏()
<220>
<223> 木聚糖生物合成酶不规则的木质部7 (IRX7) 同系物 GI:302826405
<400> 248
Gly Leu Ser Ser Asn Ile Lys Val Tyr Ile Tyr Asp Leu Pro Ser Ser
1 5 10 15
Tyr Asn Thr Asp Trp Leu Val Asp Ser Arg Cys Ser Ser His Leu Phe
20 25 30
Ala Ala Glu Val Ala Ile His Gln Asn Leu Leu Arg Ser Pro Val Arg
35 40 45
Thr Leu Asp Pro Asp Glu Ala Asp Phe Phe Phe Met Pro Val Tyr Val
50 55 60
Ser Cys Asn Phe Thr Ser Arg Ser Gly Phe Pro Thr Leu Phe His Ala
65 70 75 80
Ser Asp Ile Leu Gln Ala Ala Val Gly Leu Val Ser Arg Asn Met Pro
85 90 95
Phe Trp Asp Arg His Gln Gly Arg Asp His Val Phe Val Ala Thr His
100 105 110
Asp Phe Gly Ala Cys Phe His Ala Met Asp Leu Ala Val Thr Met Gly
115 120 125
Ile Pro Gln Phe Leu Arg Asn Ser Ile Ile Leu Gln Thr Phe Gly Glu
130 135 140
Lys Asn Lys His Pro Cys Gln Asn Val Asp His Ile Gln Ile Pro Pro
145 150 155 160
Tyr Val Arg Arg Lys Ile Leu Ala Phe Phe Arg Gly Lys Met Glu Ile
165 170 175
His Pro Lys Asn Val Ser Gly His Met Tyr Ser Arg Gly Val Arg Thr
180 185 190
Thr Ile Trp Arg Arg Phe Ser His Asp Arg Arg Phe Phe Ile Lys Arg
195 200 205
Lys Arg Ser Asp Asn Tyr Lys Ala Glu Met Leu Arg Ser Val Phe Cys
210 215 220
Leu Cys Pro Leu Gly Trp Ala Pro Trp Ser Pro Arg Ile Val Glu Ser
225 230 235 240
Val Ile Gln Gly Cys Ile Pro Val Ile Ile Ala Asp Asn Ile Gln Leu
245 250 255
Pro Tyr Ser His Val Ile Asp Trp Arg Lys Ile Ser Val Thr Val Ala
260 265 270
Glu Arg Asp Val His Lys Leu Asp Arg Ile Leu Ser Arg Val Ala Ala
275 280 285
Thr Asn Val Ser Met Ile Gln Ala Asn Leu Trp Arg Asp Glu Val Arg
290 295 300
Gln Ala Leu Val Tyr Asn Gln Pro Leu Val Arg Gly Asp Ala Thr Trp
305 310 315 320
Gln Val Leu Asp Leu Leu Ser Lys Arg Lys Asn Lys
325 330
<210> 249
<211> 415
<212> PRT
<213> 拟南芥()
<220>
<223> 木聚糖生物合成酶不规则的木质部10-like (IRX10-like, GUT1) GI:18424516
<400> 249
Met Lys Leu Ser Ser Cys Val Leu Ile Phe Leu Leu Cys Asn Thr Phe
1 5 10 15
Ser Ser Ile Ser Ala Phe Arg Leu Ser Arg Ser Gln Pro Thr Glu Arg
20 25 30
Ile Ser Gly Ser Ala Gly Asp Val Leu Glu Asp Asp Pro Val Gly Arg
35 40 45
Leu Lys Val Phe Val Tyr Glu Leu Pro Ser Lys Tyr Asn Lys Lys Ile
50 55 60
Leu Gln Lys Asp Pro Arg Cys Leu Asn His Met Phe Ala Ala Glu Ile
65 70 75 80
Tyr Met Gln Arg Phe Leu Leu Ser Ser Pro Val Arg Thr Leu Asn Pro
85 90 95
Glu Glu Ala Asp Trp Phe Tyr Val Pro Val Tyr Thr Thr Cys Asp Leu
100 105 110
Thr Pro Asn Gly Leu Pro Leu Pro Phe Lys Ser Pro Arg Met Met Arg
115 120 125
Ser Ala Ile Gln Leu Ile Ala Ser Asn Trp Pro Tyr Trp Asn Arg Thr
130 135 140
Glu Gly Ala Asp His Phe Phe Val Val Pro His Asp Phe Gly Ala Cys
145 150 155 160
Phe His Tyr Gln Glu Glu Lys Ala Ile Gly Arg Gly Ile Leu Pro Leu
165 170 175
Leu Gln Arg Ala Thr Leu Val Gln Thr Phe Gly Gln Arg Asn His Val
180 185 190
Cys Leu Lys Glu Gly Ser Ile Thr Val Pro Pro Tyr Ala Pro Pro Gln
195 200 205
Lys Met Gln Ser His Leu Ile Pro Glu Lys Thr Pro Arg Ser Ile Phe
210 215 220
Val Tyr Phe Arg Gly Leu Phe Tyr Asp Val Gly Asn Asp Pro Glu Gly
225 230 235 240
Gly Tyr Tyr Ala Arg Gly Ala Arg Ala Ala Val Trp Glu Asn Phe Lys
245 250 255
Asp Asn Pro Leu Phe Asp Ile Ser Thr Glu His Pro Thr Thr Tyr Tyr
260 265 270
Glu Asp Met Gln Arg Ala Ile Phe Cys Leu Cys Pro Leu Gly Trp Ala
275 280 285
Pro Trp Ser Pro Arg Leu Val Glu Ala Val Ile Phe Gly Cys Ile Pro
290 295 300
Val Ile Ile Ala Asp Asp Ile Val Leu Pro Phe Ala Asp Ala Ile Pro
305 310 315 320
Trp Glu Asp Ile Gly Val Phe Val Asp Glu Lys Asp Val Pro Tyr Leu
325 330 335
Asp Thr Ile Leu Thr Ser Ile Pro Pro Glu Val Ile Leu Arg Lys Gln
340 345 350
Arg Leu Leu Ala Asn Pro Ser Met Lys Gln Ala Met Leu Phe Pro Gln
355 360 365
Pro Ala Gln Pro Gly Asp Ala Phe His Gln Val Leu Asn Gly Leu Ala
370 375 380
Arg Lys Leu Pro His Glu Arg Ser Val Tyr Leu Arg Pro Gly Glu Lys
385 390 395 400
Leu Leu Asn Trp Thr Ala Gly Pro Val Ala Asp Leu Lys Pro Trp
405 410 415
<210> 250
<211> 417
<212> PRT
<213> 毛果杨()
<220>
<223> 木聚糖生物合成酶不规则的木质部10 (IRX10) 同系物 GI:224119858
<400> 250
Met Glu Ile Trp Lys Trp Val Phe Val Gly Val Leu Trp Tyr Ala Gly
1 5 10 15
Phe Val Leu Ser Thr Gly Ala Val Glu Leu Gly Arg Ser Gln His Thr
20 25 30
Glu Arg Ile Ser Gly Ser Ala Gly Asp Val Leu Glu Asp Asp Pro Val
35 40 45
Gly Arg Leu Lys Val Phe Val Tyr Glu Leu Pro Arg Lys Tyr Asn Lys
50 55 60
Lys Ile Leu Gln Lys Asp Pro Arg Cys Leu Thr His Met Phe Ala Ala
65 70 75 80
Glu Ile Phe Met His Arg Phe Leu Leu Ser Ser Pro Val Arg Thr Leu
85 90 95
Asn Pro Asp Glu Ala Asp Trp Phe Tyr Thr Pro Val Tyr Thr Thr Cys
100 105 110
Asp Leu Thr Thr Asn Gly Leu Pro Leu Pro Phe Lys Ser Pro Arg Met
115 120 125
Met Arg Ser Ala Ile Gln Leu Ile Ser Ser Asn Trp Pro Tyr Trp Asn
130 135 140
Arg Thr Glu Gly Ala Asp His Phe Phe Val Val Pro His Asp Phe Gly
145 150 155 160
Ala Cys Phe His Tyr Gln Glu Glu Lys Ala Ile Glu Arg Gly Ile Leu
165 170 175
Pro Leu Leu Gln Arg Ala Thr Leu Val Gln Thr Phe Gly Gln Arg Asn
180 185 190
His Val Cys Leu Lys Asp Gly Ser Ile Thr Val Pro Pro Tyr Ala Pro
195 200 205
Pro Gln Lys Met Gln Thr His Leu Ile Pro Glu Lys Thr Pro Arg Ser
210 215 220
Ile Phe Val Tyr Phe Arg Gly Leu Phe Tyr Asp Val Gly Asn Asp Pro
225 230 235 240
Glu Gly Gly Tyr Tyr Ala Arg Gly Ala Arg Ala Ala Val Trp Glu Asn
245 250 255
Phe Lys Asp Asn Pro Leu Phe Asp Ile Ser Thr Glu His Pro Ala Thr
260 265 270
Tyr Tyr Glu Asp Met Gln Arg Ala Val Phe Cys Leu Cys Pro Leu Gly
275 280 285
Trp Ala Pro Trp Ser Pro Arg Leu Val Glu Ala Val Ile Phe Gly Cys
290 295 300
Ile Pro Val Ile Ile Ala Asp Asp Ile Val Leu Pro Phe Ala Asp Ala
305 310 315 320
Ile Pro Trp Glu Glu Ile Gly Val Tyr Val Asp Glu Glu Asp Val Pro
325 330 335
Asn Leu Asp Thr Ile Leu Thr Ser Ile Pro Pro Glu Val Ile Leu Arg
340 345 350
Lys Gln Arg Leu Leu Ala Asn Pro Ser Met Lys Gln Ala Met Leu Phe
355 360 365
Pro Gln Pro Ala Gln Pro Gly Asp Ala Phe His Gln Val Leu Asn Gly
370 375 380
Leu Ala Arg Lys Leu Pro His Asp Arg Ser Val Tyr Leu Arg Pro Gly
385 390 395 400
Glu Lys Ile Leu Asn Trp Thr Ala Gly Pro Val Gly Asp Leu Lys Pro
405 410 415
Trp
<210> 251
<211> 412
<212> PRT
<213> 拟南芥()
<220>
<223> 木聚糖生物合成酶不规则的木质部10 (IRX10, GUT2) GI:15223522
<400> 251
Met Lys Ile His Ser Cys Leu Ser Ala Ile Leu Leu Phe Leu Phe Phe
1 5 10 15
Ser Ala Ser Ser Ala Lys Gln Asn Val Arg Thr Glu Arg Ile Ser Gly
20 25 30
Ser Ala Gly Asp Val Leu Glu Asp Asp Pro Val Gly Lys Leu Lys Val
35 40 45
Tyr Val Tyr Glu Leu Pro Ser Lys Tyr Asn Lys Lys Leu Leu Gln Lys
50 55 60
Asp Pro Arg Cys Leu Thr His Met Phe Ala Ala Glu Ile Phe Met His
65 70 75 80
Arg Phe Leu Leu Ser Ser Pro Val Arg Thr Arg Asn Pro Asp Glu Ala
85 90 95
Asp Trp Phe Tyr Thr Pro Ile Tyr Pro Thr Cys Asp Leu Thr Pro Thr
100 105 110
Gly Leu Pro Leu Pro Phe Lys Ser Pro Arg Met Met Arg Ser Ser Ile
115 120 125
Gln Leu Ile Ser Ser Asn Trp Pro Tyr Trp Asn Arg Thr Glu Gly Ala
130 135 140
Asp His Phe Phe Val Val Pro His Asp Phe Gly Ala Cys Phe His Tyr
145 150 155 160
Gln Glu Glu Lys Ala Ile Glu Arg Gly Ile Leu Pro Leu Leu Gln Arg
165 170 175
Ala Thr Leu Val Gln Thr Phe Gly Gln Arg Asn His Val Cys Leu Asp
180 185 190
Glu Gly Ser Ile Thr Ile Pro Pro Phe Ala Pro Pro Gln Lys Met Gln
195 200 205
Ala His Phe Ile Pro Pro Asp Ile Pro Arg Ser Ile Phe Val Tyr Phe
210 215 220
Arg Gly Leu Phe Tyr Asp Val Asn Asn Asp Pro Glu Gly Gly Tyr Tyr
225 230 235 240
Ala Arg Gly Ala Arg Ala Ala Val Trp Glu Asn Phe Lys Asn Asn Pro
245 250 255
Leu Phe Asp Ile Ser Thr Asp His Pro Thr Thr Tyr Tyr Glu Asp Met
260 265 270
Gln Arg Ala Ile Phe Cys Leu Cys Pro Leu Gly Trp Ala Pro Trp Ser
275 280 285
Pro Arg Leu Val Glu Ala Val Val Phe Gly Cys Ile Pro Val Ile Ile
290 295 300
Ala Asp Asp Ile Val Leu Pro Phe Ala Asp Ala Ile Pro Trp Glu Glu
305 310 315 320
Ile Gly Val Phe Val Ala Glu Lys Asp Val Pro Glu Leu Asp Thr Ile
325 330 335
Leu Thr Ser Ile Pro Thr Glu Val Ile Leu Arg Lys Gln Arg Leu Leu
340 345 350
Ala Asn Pro Ser Met Lys Arg Ala Met Leu Phe Pro Gln Pro Ala Gln
355 360 365
Pro Gly Asp Ala Phe His Gln Ile Leu Asn Gly Leu Ala Arg Lys Leu
370 375 380
Pro His Asp Lys Ser Ile Tyr Leu Lys Thr Gly Glu Lys Ala Leu Asn
385 390 395 400
Trp Thr Ala Gly Pro Val Ala Asp Leu Lys Pro Trp
405 410
<210> 252
<211> 412
<212> PRT
<213> 毛果杨()
<220>
<223> 木聚糖生物合成酶不规则的木质部10 (IRX10) 同系物 GI:224053575
<400> 252
Met Arg Thr Cys Leu Trp Val Phe Ala Val Val Leu Val Phe Gly Phe
1 5 10 15
Val Asp Gly Lys Glu Ile Glu Arg Leu Arg Thr Glu Arg Ile Ser Gly
20 25 30
Ser Ala Gly Asp Val Leu Asp Asp Asp Pro Val Gly Arg Leu Lys Val
35 40 45
Tyr Val Tyr Glu Leu Pro Ser Lys Tyr Asn Lys Lys Leu Leu Gln Lys
50 55 60
Asp Pro Arg Cys Leu Thr His Met Phe Ala Ala Glu Ile Phe Met His
65 70 75 80
Arg Phe Leu Leu Ser Ser Pro Val Arg Thr Leu Asn Pro Asp Glu Ala
85 90 95
Asp Trp Phe Tyr Ser Pro Ile Tyr Pro Thr Cys Asp Leu Thr Pro Met
100 105 110
Gly Leu Pro Leu Pro Phe Lys Ser Pro Arg Met Met Arg Ser Ala Ile
115 120 125
Gln Leu Ile Ser Ser Asn Trp Pro Tyr Trp Asn Arg Thr Glu Gly Ala
130 135 140
Asp His Phe Phe Val Val Pro His Asp Phe Gly Ala Cys Phe His Tyr
145 150 155 160
Gln Glu Glu Lys Ala Ile Glu Arg Gly Ile Leu Pro Leu Leu Gln Arg
165 170 175
Ser Thr Leu Val Gln Thr Phe Gly Gln Arg Asn His Val Cys Leu Asn
180 185 190
Glu Gly Ser Ile Thr Ile Pro Pro Phe Ala Pro Pro Gln Lys Met Gln
195 200 205
Ala His Gln Ile Pro Pro Asp Ile Pro Arg Ser Ile Phe Val Tyr Phe
210 215 220
Arg Gly Leu Phe Tyr Asp Val Asn Asn Asp Pro Glu Gly Gly Tyr Tyr
225 230 235 240
Ala Arg Gly Ala Arg Ala Ala Val Trp Glu Asn Phe Lys Asn Asn Pro
245 250 255
Leu Phe Asp Ile Ser Thr Asp His Pro Thr Thr Tyr Tyr Glu Asp Met
260 265 270
Gln Arg Ala Ile Phe Cys Leu Cys Pro Leu Gly Trp Ala Pro Trp Ser
275 280 285
Pro Arg Leu Val Glu Ala Val Val Phe Gly Cys Ile Pro Val Ile Ile
290 295 300
Ala Asp Asp Ile Val Leu Pro Phe Ala Asp Ala Ile Pro Trp Glu Glu
305 310 315 320
Ile Gly Val Phe Val Ala Glu Glu Asp Val Pro Asn Leu Asp Thr Ile
325 330 335
Leu Thr Ser Ile Pro Pro Glu Val Ile Leu Arg Lys Gln Arg Leu Leu
340 345 350
Ala Asn Pro Ser Met Lys Arg Ala Met Leu Phe Pro Gln Pro Ala Gln
355 360 365
Pro Gly Asp Ala Phe His Gln Ile Leu Asn Gly Leu Ala Arg Lys Leu
370 375 380
Pro His Asp Arg Ser Val Tyr Leu Lys Ser Gly Gln Asn Ile Leu Asn
385 390 395 400
Trp Thr Ala Gly Pro Val Gly Asp Leu Lys Pro Trp
405 410
<210> 253
<211> 413
<212> PRT
<213> 毛果杨()
<220>
<223> 木聚糖生物合成酶不规则的木质部10 (IRX10) 同系物 GI:224075447
<400> 253
Met Arg Val Cys Leu Trp Phe Phe Val Val Val Ile Val Phe Gly Phe
1 5 10 15
Gly Asp Gly Lys Asn Ala Asp Gln Arg Leu Arg Thr Glu Arg Ile Ser
20 25 30
Gly Ser Ala Gly Asp Val Leu Glu Asp Asn Pro Val Gly Arg Leu Lys
35 40 45
Val Phe Val Tyr Glu Leu Pro Ser Lys Tyr Asn Lys Lys Leu Leu Gln
50 55 60
Lys Asp Pro Arg Cys Leu Thr His Met Phe Ala Ala Glu Ile Phe Met
65 70 75 80
His Arg Phe Leu Leu Ser Ser Pro Val Arg Thr Leu Asn Pro Glu Glu
85 90 95
Ala Asp Trp Phe Tyr Thr Pro Ile Tyr Pro Thr Cys Asp Leu Thr Pro
100 105 110
Thr Gly Leu Pro Leu Pro Phe Asn Ser Pro Arg Met Met Arg Ser Ala
115 120 125
Ile Gln Leu Leu Ser Ser Asn Trp Pro Tyr Trp Asn Arg Thr Glu Gly
130 135 140
Ala Asp His Phe Phe Val Val Pro His Asp Phe Gly Ala Cys Phe His
145 150 155 160
Tyr Gln Glu Glu Lys Ala Ile Glu Arg Gly Ile Leu Pro Leu Leu Arg
165 170 175
Arg Ala Thr Leu Val Gln Thr Phe Gly Gln Arg Asn His Val Cys Leu
180 185 190
Asn Glu Gly Ser Ile Thr Ile Pro Pro Tyr Ala Pro Pro Gln Lys Met
195 200 205
Gln Ala His Gln Ile Pro Leu Asp Thr Pro Arg Ser Ile Phe Val Tyr
210 215 220
Phe Arg Gly Leu Phe Tyr Asp Val Asn Asn Asp Pro Glu Gly Gly Tyr
225 230 235 240
Tyr Ala Arg Gly Ala Arg Ala Ala Val Trp Glu Asn Phe Lys Asn Asn
245 250 255
Pro Leu Phe Asp Ile Ser Thr Asp His Pro Thr Thr Tyr Tyr Glu Asp
260 265 270
Met Gln Arg Ala Ile Phe Cys Leu Cys Pro Leu Gly Trp Ala Pro Trp
275 280 285
Ser Pro Arg Leu Val Glu Ala Val Val Phe Gly Cys Ile Pro Val Ile
290 295 300
Ile Ala Asp Asp Ile Val Leu Pro Phe Ala Asp Ala Ile Pro Trp Glu
305 310 315 320
Glu Ile Gly Val Phe Val Ala Glu Glu Asp Val Pro His Leu Asp Thr
325 330 335
Phe Leu Thr Ser Ile Pro Pro Glu Val Ile Leu Arg Lys Gln Arg Leu
340 345 350
Leu Ala Asn Pro Ser Met Lys Arg Ala Met Leu Phe Pro Gln Pro Ala
355 360 365
Gln Pro Gly Asp Ala Phe His Gln Ile Leu Asn Gly Leu Ala Arg Lys
370 375 380
Leu Pro His Asp Arg Thr Val Tyr Leu Lys Pro Gly Gln Lys Val Leu
385 390 395 400
Asn Trp Thr Ala Gly Pro Ala Gly Asp Leu Lys Pro Trp
405 410
<210> 254
<211> 415
<212> PRT
<213> 水稻()
<220>
<223> 木聚糖生物合成酶不规则的木质部10 (IRX10) 同系物 GI:115441967,Os01g0926600
<400> 254
Met Ala Met Arg Leu Ser Ser Ala Ala Val Ala Leu Ala Leu Leu Leu
1 5 10 15
Ala Ala Thr Ala Leu Glu Asp Val Ala Arg Gly Gln Asp Thr Glu Arg
20 25 30
Ile Glu Gly Ser Ala Gly Asp Val Leu Glu Asp Asp Pro Val Gly Arg
35 40 45
Leu Lys Val Tyr Val Tyr Glu Leu Pro Thr Lys Tyr Asn Lys Lys Met
50 55 60
Val Ala Lys Asp Ser Arg Cys Leu Ser His Met Phe Ala Ala Glu Ile
65 70 75 80
Phe Met His Arg Phe Leu Leu Ser Ser Ala Ile Arg Thr Leu Asn Pro
85 90 95
Glu Glu Ala Asp Trp Phe Tyr Thr Pro Val Tyr Thr Thr Cys Asp Leu
100 105 110
Thr Pro Trp Gly His Pro Leu Pro Phe Lys Ser Pro Arg Ile Met Arg
115 120 125
Ser Ala Ile Gln Phe Ile Ser Ser His Trp Pro Tyr Trp Asn Arg Thr
130 135 140
Asp Gly Ala Asp His Phe Phe Val Val Pro His Asp Phe Gly Ala Cys
145 150 155 160
Phe His Tyr Gln Glu Glu Lys Ala Ile Glu Arg Gly Ile Leu Pro Leu
165 170 175
Leu Arg Arg Ala Thr Leu Val Gln Thr Phe Gly Gln Lys Asp His Val
180 185 190
Cys Leu Lys Glu Gly Ser Ile Thr Ile Pro Pro Tyr Ala Pro Pro Gln
195 200 205
Lys Met Lys Thr His Leu Val Pro Pro Glu Thr Pro Arg Ser Ile Phe
210 215 220
Val Tyr Phe Arg Gly Leu Phe Tyr Asp Thr Ala Asn Asp Pro Glu Gly
225 230 235 240
Gly Tyr Tyr Ala Arg Gly Ala Arg Ala Ser Val Trp Glu Asn Phe Lys
245 250 255
Asn Asn Pro Leu Phe Asp Ile Ser Thr Asp His Pro Pro Thr Tyr Tyr
260 265 270
Glu Asp Met Gln Arg Ser Ile Phe Cys Leu Cys Pro Leu Gly Trp Ala
275 280 285
Pro Trp Ser Pro Arg Leu Val Glu Ala Val Val Phe Gly Cys Ile Pro
290 295 300
Val Ile Ile Ala Asp Asp Ile Val Leu Pro Phe Ala Asp Ala Ile Pro
305 310 315 320
Trp Asp Glu Ile Gly Val Phe Val Ala Glu Asp Asp Val Pro Lys Leu
325 330 335
Asp Thr Ile Leu Thr Ser Ile Pro Met Asp Val Ile Leu Arg Lys Gln
340 345 350
Arg Leu Leu Ala Asn Pro Ser Met Lys Gln Ala Met Leu Phe Pro Gln
355 360 365
Pro Ala Gln Pro Gly Asp Ala Phe His Gln Ile Leu Asn Gly Leu Gly
370 375 380
Arg Lys Leu Pro His Pro Lys Ser Val Tyr Leu Asp Pro Gly Gln Lys
385 390 395 400
Val Leu Asn Trp Thr Gln Gly Pro Val Gly Asp Leu Lys Pro Trp
405 410 415
<210> 255
<211> 437
<212> PRT
<213> 江南卷柏()
<220>
<223> 木聚糖生物合成酶不规则的木质部10 (IRX10) 同系物 GI:302783378,GT47D1
<400> 255
Met Gly Ala Ala Trp Ile Ala Leu Ala Leu Gly Leu Ala Leu Cys Ala
1 5 10 15
Ser Ala Glu Gln Gln Gln Gln His Glu Arg Ile Ser Gly Ser Ala Gly
20 25 30
Asp Val Leu Glu Asp Asp Pro Val Gly Arg Leu Lys Val Phe Val Tyr
35 40 45
Glu Leu Pro Val Lys Tyr Asn Arg Lys Val Leu Glu Lys Asp Pro Arg
50 55 60
Cys Leu Thr His Met Phe Ala Thr Glu Ile Phe Val His Glu Phe Leu
65 70 75 80
Leu Gly Ser Ala Val Arg Thr Leu Asn Pro Glu Glu Ala Asp Trp Phe
85 90 95
Tyr Thr Pro Val Tyr Thr Thr Cys Asp Leu Thr Arg Asn Gly Leu Pro
100 105 110
Leu Pro Phe Lys Ser Pro Arg Met Met Arg Ser Val Ile Gln Tyr Ile
115 120 125
Ser Asn Gln Trp Pro Tyr Trp Asn Arg Thr Glu Gly Ala Asp His Phe
130 135 140
Phe Val Val Pro His Asp Phe Gly Ala Cys Phe His Tyr Gln Glu Glu
145 150 155 160
Lys Ala Ile Glu Arg Gly Ile Leu His Leu Leu Gln Arg Ala Thr Leu
165 170 175
Val Gln Thr Phe Gly Gln Arg Tyr His Val Cys Leu Lys Glu Gly Ser
180 185 190
Ile Val Val Pro Pro Tyr Cys Pro Pro Gln Lys Met Gln Ala His Leu
195 200 205
Ile Pro Pro Ser Ile Pro Arg Ser Ile Phe Val Tyr Phe Arg Gly Leu
210 215 220
Phe Tyr Asp Tyr Gly Asn Asp Pro Glu Gly Gly Tyr Tyr Ala Arg Gly
225 230 235 240
Ala Arg Ala Ala Val Trp Glu Asn Phe Lys Asp Asn Pro Leu Phe Asp
245 250 255
Ile Ser Thr Glu His Pro Ile Thr Tyr Tyr Glu Asp Met Gln Arg Ala
260 265 270
Ile Phe Cys Leu Cys Pro Leu Gly Trp Ala Pro Trp Ser Pro Arg Leu
275 280 285
Val Glu Gly Val Ile Phe Gly Cys Ile Pro Val Ile Ile Ala Asp Asp
290 295 300
Ile Val Leu Pro Phe Ala Asp Ala Ile Pro Trp Glu Glu Ile Gly Val
305 310 315 320
Phe Val Ala Glu Lys Asp Val Pro Lys Leu Asp Thr Ile Leu Thr Ser
325 330 335
Ile Pro Pro Glu Val Ile Leu Lys Lys Gln Arg Leu Leu Ala Thr Pro
340 345 350
Ala Met Lys Gln Ala Met Leu Phe Pro Gln Pro Ala Gln Pro Gly Asp
355 360 365
Ala Phe His Gln Ile Leu Asn Gly Leu Ala Arg Lys Leu Pro His Asp
370 375 380
Arg Arg Ile Tyr Leu Asn Pro Gly Glu Lys Ala Leu Asn Trp Ser Ser
385 390 395 400
Gly Pro Pro Gly Asp Leu Lys Pro Cys Lys Arg Asn Thr Leu Asp Arg
405 410 415
Glu Ser Arg Asn Trp His Gln Leu Leu Gln Leu Leu Arg His Trp Cys
420 425 430
Arg Val Leu Leu Phe
435
<210> 256
<211> 420
<212> PRT
<213> 水稻()
<220>
<223> 木聚糖生物合成酶不规则的木质部10 (IRX10) 同系物 GI:115458146,Os04g0398600
<400> 256
Met Gly Ser Arg Thr Val Gly Trp Trp Leu Leu Ala Ala Ala Val Val
1 5 10 15
Leu Ala Ala Ala Ala Ala Asp Ser Gly Glu Ala Glu Arg Ala Ala Glu
20 25 30
Gln His Ser Glu Arg Ile Ser Gly Ser Ala Gly Asp Val Leu Glu Asp
35 40 45
Asn Pro Val Gly Arg Leu Lys Val Phe Ile Tyr Asp Leu Pro Arg Lys
50 55 60
Tyr Asn Lys Lys Met Val Asn Lys Asp Pro Arg Cys Leu Asn His Met
65 70 75 80
Phe Ala Ala Glu Ile Phe Met His Arg Phe Leu Leu Ser Ser Ala Val
85 90 95
Arg Thr Leu Asn Pro Lys Glu Ala Asp Trp Phe Tyr Thr Pro Val Tyr
100 105 110
Thr Thr Cys Asp Leu Thr Pro Ala Gly Leu Pro Leu Pro Phe Lys Ser
115 120 125
Pro Arg Val Met Arg Ser Ala Ile Gln Tyr Ile Ser His Lys Trp Pro
130 135 140
Phe Trp Asn Arg Thr Asp Gly Ala Asp His Phe Phe Val Val Pro His
145 150 155 160
Asp Phe Gly Ala Cys Phe His Tyr Gln Glu Glu Lys Ala Ile Glu Arg
165 170 175
Gly Ile Leu Pro Leu Leu Gln Arg Ala Thr Leu Val Gln Thr Phe Gly
180 185 190
Gln Glu Asn His Val Cys Leu Lys Glu Gly Ser Ile Thr Ile Pro Pro
195 200 205
Tyr Ala Pro Pro Gln Lys Met Gln Ala His Leu Ile Pro Pro Asp Thr
210 215 220
Pro Arg Ser Ile Phe Val Tyr Phe Arg Gly Leu Phe Tyr Asp Thr Gly
225 230 235 240
Asn Asp Pro Glu Gly Gly Tyr Tyr Ala Arg Gly Ala Arg Ala Ser Leu
245 250 255
Trp Glu Asn Phe Lys Asn Asn Pro Leu Phe Asp Ile Ser Thr Asp His
260 265 270
Pro Pro Thr Tyr Tyr Glu Asp Met Gln Arg Ala Val Phe Cys Leu Cys
275 280 285
Pro Leu Gly Trp Ala Pro Trp Ser Pro Arg Leu Val Glu Ala Val Val
290 295 300
Phe Gly Cys Ile Pro Val Ile Ile Ala Asp Asp Ile Val Leu Pro Phe
305 310 315 320
Ala Asp Ala Ile Pro Trp Glu Glu Ile Gly Val Phe Val Glu Glu Lys
325 330 335
Asp Val Pro Lys Leu Asp Thr Ile Leu Thr Ser Met Pro Ile Asp Asp
340 345 350
Ile Leu Arg Lys Gln Arg Leu Leu Ala Asn Pro Ser Met Lys Gln Ala
355 360 365
Met Leu Phe Pro Gln Pro Ala Gln Pro Arg Asp Ala Phe His Gln Ile
370 375 380
Leu Asn Gly Leu Ala Arg Lys Leu Pro His Pro Glu Gly Val Tyr Leu
385 390 395 400
Gln Pro Ser Asp Lys Arg Leu Asn Trp Thr Ala Gly Pro Val Gly Asp
405 410 415
Leu Lys Ala Trp
420
<210> 257
<211> 422
<212> PRT
<213> 水稻()
<220>
<223> 木聚糖生物合成酶不规则的木质部10 (IRX10) 同系物 GI:115441965,Os01g0926400
<400> 257
Met Gly Thr Arg Pro Cys Ala Gly Val Ala Ser Ala Val Ala Ala Ala
1 5 10 15
Val Ala Val Leu Leu Leu Ala Val Ser Cys Phe Ala Ala Ala Ala Thr
20 25 30
Thr Thr Gln Lys His Gly Arg Met Ser Gly Lys Gly Gly Asp Val Leu
35 40 45
Glu Asp Asp Pro Thr Gly Lys Leu Lys Val Phe Val Tyr Glu Met Pro
50 55 60
Arg Lys Tyr Asn Leu Asn Leu Leu Ala Lys Asp Ser Arg Cys Leu Gln
65 70 75 80
His Met Phe Ala Ala Glu Ile Phe Met His Gln Phe Leu Leu Ser Ser
85 90 95
Pro Val Arg Thr Leu Asp Pro Glu Glu Ala Asp Trp Phe Tyr Thr Pro
100 105 110
Ala Tyr Thr Thr Cys Asp Leu Thr Pro Gln Gly Phe Pro Leu Pro Phe
115 120 125
Arg Ala Pro Arg Ile Met Arg Ser Ala Val Arg Tyr Val Ala Ala Thr
130 135 140
Trp Pro Tyr Trp Asn Arg Thr Asp Gly Ala Asp His Phe Phe Leu Ala
145 150 155 160
Pro His Asp Phe Gly Ala Cys Phe His Tyr Gln Glu Glu Arg Ala Ile
165 170 175
Glu Arg Gly Ile Leu Pro Val Leu Arg Arg Ala Thr Leu Val Gln Thr
180 185 190
Phe Gly Gln Arg His His Pro Cys Leu Gln Pro Gly Ser Ile Thr Val
195 200 205
Pro Pro Tyr Ala Asp Pro Arg Lys Met Glu Ala His Arg Ile Ser Pro
210 215 220
Ala Thr Pro Arg Ser Ile Phe Val Tyr Phe Arg Gly Leu Phe Tyr Asp
225 230 235 240
Met Gly Asn Asp Pro Glu Gly Gly Tyr Tyr Ala Arg Gly Ala Arg Ala
245 250 255
Ser Val Trp Glu Asn Phe Lys Asp Asn Pro Leu Phe Asp Ile Ser Thr
260 265 270
Glu His Pro Ala Thr Tyr Tyr Glu Asp Met Gln Arg Ala Ile Phe Cys
275 280 285
Leu Cys Pro Leu Gly Trp Ala Pro Trp Ser Pro Arg Leu Val Glu Ala
290 295 300
Val Val Phe Gly Cys Ile Pro Val Ile Ile Ala Asp Asp Ile Val Leu
305 310 315 320
Pro Phe Ala Asp Ala Ile Pro Trp Gly Glu Ile Ser Val Phe Val Ala
325 330 335
Glu Glu Asp Val Pro Arg Leu Asp Thr Ile Leu Ala Ser Val Pro Leu
340 345 350
Asp Glu Val Ile Arg Lys Gln Arg Leu Leu Ala Ser Pro Ala Met Lys
355 360 365
Gln Ala Val Leu Phe His Gln Pro Ala Arg Pro Gly Asp Ala Phe His
370 375 380
Gln Ile Leu Asn Gly Leu Ala Arg Lys Leu Pro His Pro Lys Gly Val
385 390 395 400
Phe Leu Glu Pro Gly Glu Lys Gly Ile Asp Trp Asp Gln Gly Leu Glu
405 410 415
Asn Asp Leu Lys Pro Trp
420
<210> 258
<211> 417
<212> PRT
<213> 水稻()
<220>
<223> 木聚糖生物合成酶不规则的木质部10 (IRX10) 同系物 GI:115481310,Os10g0180000
<400> 258
Met Gly Thr Arg Arg Arg Ser Ala Arg Ala Arg Ala Arg Pro Pro Leu
1 5 10 15
Ala Met Pro Leu Ala Val Leu Leu Leu Phe Ala Cys Ser Ser Gly Val
20 25 30
Ala Ala Ala Ala Ala Gln Gly Ile Glu Arg Ile Lys Asp Asp Pro Val
35 40 45
Gly Lys Leu Lys Val Tyr Val Tyr Glu Leu Pro Pro Lys Tyr Asn Lys
50 55 60
Asn Ile Val Ala Lys Asp Ser Arg Cys Leu Ser His Met Phe Ala Thr
65 70 75 80
Glu Ile Phe Met His Arg Phe Leu Leu Ser Ser Ala Ile Arg Thr Ser
85 90 95
Asn Pro Asp Glu Ala Asp Trp Phe Tyr Thr Pro Val Tyr Thr Thr Cys
100 105 110
Asp Leu Thr Pro Trp Gly His Pro Leu Thr Thr Lys Ser Pro Arg Met
115 120 125
Met Arg Ser Ala Ile Lys Phe Ile Ser Lys Tyr Trp Pro Tyr Trp Asn
130 135 140
Arg Thr Glu Gly Ala Asp His Phe Phe Val Val Pro His Asp Phe Ala
145 150 155 160
Ala Cys Phe Tyr Phe Gln Glu Ala Lys Ala Ile Glu Arg Gly Ile Leu
165 170 175
Pro Val Leu Arg Arg Ala Thr Leu Val Gln Thr Phe Gly Gln Lys Asn
180 185 190
His Ala Cys Leu Lys Asp Gly Ser Ile Thr Val Pro Pro Tyr Thr Pro
195 200 205
Ala His Lys Ile Arg Ala His Leu Val Pro Pro Glu Thr Pro Arg Ser
210 215 220
Ile Phe Val Tyr Phe Arg Gly Leu Phe Tyr Asp Thr Ser Asn Asp Pro
225 230 235 240
Glu Gly Gly Tyr Tyr Ala Arg Gly Ala Arg Ala Ser Val Trp Glu Asn
245 250 255
Phe Lys Asn Asn Pro Met Phe Asp Ile Ser Thr Asp His Pro Gln Thr
260 265 270
Tyr Tyr Glu Asp Met Gln Arg Ala Val Phe Cys Leu Cys Pro Leu Gly
275 280 285
Trp Ala Pro Trp Ser Pro Arg Leu Val Glu Ala Val Val Phe Gly Cys
290 295 300
Ile Pro Val Ile Ile Ala Asp Asp Ile Val Leu Pro Phe Ser Asp Ala
305 310 315 320
Ile Pro Trp Glu Glu Ile Ala Val Phe Val Ala Glu Asp Asp Val Pro
325 330 335
Gln Leu Asp Thr Ile Leu Thr Ser Ile Pro Thr Glu Val Ile Leu Arg
340 345 350
Lys Gln Ala Met Leu Ala Glu Pro Ser Met Lys Gln Thr Met Leu Phe
355 360 365
Pro Gln Pro Ala Glu Pro Gly Asp Gly Phe His Gln Val Met Asn Ala
370 375 380
Leu Ala Arg Lys Leu Pro His Gly Arg Asp Val Phe Leu Lys Pro Gly
385 390 395 400
Gln Lys Val Leu Asn Trp Thr Glu Gly Thr Arg Glu Asp Leu Lys Pro
405 410 415
Trp
<210> 259
<211> 462
<212> PRT
<213> 毛果杨()
<220>
<223> 木聚糖生物合成酶不规则的木质部10 (IRX10) 同系物 GI:224106838
<400> 259
Met Val Leu Glu Val Lys Arg Pro Thr Met Ser Asn Asn Arg Arg Gly
1 5 10 15
Phe Tyr Val Arg Met Lys Leu Leu His Arg His Ala Gly Leu Gln Gln
20 25 30
Gln Glu Lys Lys Lys Asn Leu Cys Phe Lys Tyr Tyr Lys Trp Leu Leu
35 40 45
Trp Phe Ser Leu Leu Leu Tyr Phe Leu Ser Ser Cys Phe Phe Thr His
50 55 60
Lys Pro Ile Pro Leu Ser Lys Thr His Val Ser Glu Ser Lys Thr Val
65 70 75 80
Val Ser Arg Ala Leu Phe Glu Ser Ser Asn Ser Thr Phe Ile Gln Gln
85 90 95
Ser Lys Asn Ile Asn Arg Gly Leu Leu Lys Asp Leu Lys Val Tyr Ile
100 105 110
Tyr Glu Leu Pro Ser Lys Tyr Asn Thr Asp Trp Leu Ala Asn Glu Arg
115 120 125
Cys Ser Asn His Leu Phe Ala Ser Glu Val Ala Ile His Lys Ala Leu
130 135 140
Ser Asn Ser Leu Asp Ile Arg Thr Phe Asp Pro Tyr Glu Ala Asp Phe
145 150 155 160
Phe Phe Val Pro Val Tyr Val Ser Cys Asn Phe Ser Thr Val Asn Gly
165 170 175
Phe Pro Ala Ile Gly His Ala Arg Ser Leu Leu Ser Ser Ala Val Gln
180 185 190
Leu Ile Ser Ser Asn Tyr Pro Phe Trp Asn Arg Ser Gln Gly Ser Asp
195 200 205
His Val Phe Val Ala Ser His Asp Tyr Gly Ala Cys Phe His Ala Met
210 215 220
Glu Glu Arg Ala Met Glu Asp Gly Ile Pro Glu Phe Leu Lys Arg Ser
225 230 235 240
Ile Ile Leu Gln Thr Phe Gly Val Lys Phe Asn His Pro Cys Gln Asp
245 250 255
Val Glu Asn Val Val Ile Pro Pro Tyr Ile Ser Pro Glu Arg Val Arg
260 265 270
Thr Thr Leu Glu Asn Tyr Pro Leu Asn Gly Arg Arg Asp Ile Trp Ala
275 280 285
Phe Phe Arg Gly Lys Met Glu Val His Pro Lys Asn Ile Ser Gly Arg
290 295 300
Tyr Tyr Ser Lys Lys Val Arg Thr Val Ile Trp Arg Lys Tyr Ser Gly
305 310 315 320
Asp Arg Arg Phe Tyr Leu Gln Arg His Arg Phe Ala Gly Tyr Gln Ser
325 330 335
Glu Ile Val Arg Ser Val Phe Cys Leu Cys Pro Leu Gly Trp Ala Pro
340 345 350
Trp Ser Pro Arg Leu Val Glu Ser Val Ala Leu Gly Cys Val Pro Val
355 360 365
Ile Ile Ala Asp Gly Ile Arg Leu Pro Phe Pro Thr Ala Val Arg Trp
370 375 380
Ser Glu Ile Ser Leu Thr Val Ala Glu Lys Asp Val Ala Asn Leu Gly
385 390 395 400
Thr Leu Leu Asp His Val Ala Ala Thr Asn Leu Ser Ala Ile Gln Lys
405 410 415
Asn Leu Trp Asp Pro Asp Val Arg Arg Ala Leu Leu Phe Asn Asp Arg
420 425 430
Val Gln Glu Gly Asp Ala Thr Trp Gln Val Leu Tyr Ala Leu Ala Arg
435 440 445
Lys Leu Asp Arg Ser Tyr Arg Thr Val Arg Leu Pro Asn Gln
450 455 460
<210> 260
<211> 351
<212> PRT
<213> 拟南芥()
<220>
<223> 木聚糖生物合成酶PARVUS (GATL1) GI:18394719
<400> 260
Met Ser Gln His Leu Leu Leu Leu Ile Leu Leu Ser Leu Leu Leu Leu
1 5 10 15
His Lys Pro Ile Ser Ala Thr Thr Ile Ile Gln Lys Phe Lys Glu Ala
20 25 30
Pro Gln Phe Tyr Asn Ser Ala Asp Cys Pro Leu Ile Asp Asp Ser Glu
35 40 45
Ser Asp Asp Asp Val Val Ala Lys Pro Ile Phe Cys Ser Arg Arg Ala
50 55 60
Val His Val Ala Met Thr Leu Asp Ala Ala Tyr Ile Arg Gly Ser Val
65 70 75 80
Ala Ala Val Leu Ser Val Leu Gln His Ser Ser Cys Pro Glu Asn Ile
85 90 95
Val Phe His Phe Val Ala Ser Ala Ser Ala Asp Ala Ser Ser Leu Arg
100 105 110
Ala Thr Ile Ser Ser Ser Phe Pro Tyr Leu Asp Phe Thr Val Tyr Val
115 120 125
Phe Asn Val Ser Ser Val Ser Arg Leu Ile Ser Ser Ser Ile Arg Ser
130 135 140
Ala Leu Asp Cys Pro Leu Asn Tyr Ala Arg Ser Tyr Leu Ala Asp Leu
145 150 155 160
Leu Pro Pro Cys Val Arg Arg Val Val Tyr Leu Asp Ser Asp Leu Ile
165 170 175
Leu Val Asp Asp Ile Ala Lys Leu Ala Ala Thr Asp Leu Gly Arg Asp
180 185 190
Ser Val Leu Ala Ala Pro Glu Tyr Cys Asn Ala Asn Phe Thr Ser Tyr
195 200 205
Phe Thr Ser Thr Phe Trp Ser Asn Pro Thr Leu Ser Leu Thr Phe Ala
210 215 220
Asp Arg Lys Ala Cys Tyr Phe Asn Thr Gly Val Met Val Ile Asp Leu
225 230 235 240
Ser Arg Trp Arg Glu Gly Ala Tyr Thr Ser Arg Ile Glu Glu Trp Met
245 250 255
Ala Met Gln Lys Arg Met Arg Ile Tyr Glu Leu Gly Ser Leu Pro Pro
260 265 270
Phe Leu Leu Val Phe Ala Gly Leu Ile Lys Pro Val Asn His Arg Trp
275 280 285
Asn Gln His Gly Leu Gly Gly Asp Asn Phe Arg Gly Leu Cys Arg Asp
290 295 300
Leu His Pro Gly Pro Val Ser Leu Leu His Trp Ser Gly Lys Gly Lys
305 310 315 320
Pro Trp Ala Arg Leu Asp Ala Gly Arg Pro Cys Pro Leu Asp Ala Leu
325 330 335
Trp Ala Pro Tyr Asp Leu Leu Gln Thr Pro Phe Ala Leu Asp Ser
340 345 350
<210> 261
<211> 353
<212> PRT
<213> 毛果杨()
<220>
<223> 木聚糖生物合成酶PARVUS同系物 GI:224067335
<400> 261
Met Leu Met Pro Arg Leu Ile His Lys Leu Thr Leu Leu Phe Leu Phe
1 5 10 15
Leu Ser Leu Thr Thr Ala Thr Ile Thr Gln Gln Phe Lys Glu Ala Pro
20 25 30
Gln Phe Tyr Asn Ser Pro Asp Cys His Ser Ile Asp Gln Asp Gly Ile
35 40 45
Asp Ser Asp Ser Glu Ser Asp Gly Asp Lys Thr Ile Phe Cys Ser Glu
50 55 60
His Ala Val His Val Ala Met Thr Leu Asp Thr Ala Tyr Ile Arg Gly
65 70 75 80
Ser Met Ala Ala Ile Leu Ser Val Leu Gln His Thr Ser Cys Pro Gln
85 90 95
Asn Ile Ala Phe His Phe Val Ala Ser Ala Ser Ala Asn Thr Ser Leu
100 105 110
Leu Arg Ala Thr Ile Ser Ser Ser Phe Pro Tyr Leu Asn Phe Arg Val
115 120 125
Tyr Thr Phe Asp Asp Ser Ser Val Ser Arg Leu Ile Ser Thr Ser Ile
130 135 140
Arg Ser Ala Leu Asp Cys Pro Leu Asn Tyr Ala Arg Ser Tyr Leu Ala
145 150 155 160
Asn Ile Ile Pro Leu Cys Val Arg Arg Val Val Tyr Leu Asp Ser Asp
165 170 175
Leu Val Leu Val Asp Asp Ile Ala Lys Leu Ala Ala Thr Pro Leu Gly
180 185 190
Glu Gln Ser Val Leu Ala Ala Pro Glu Tyr Cys Asn Ala Asn Phe Thr
195 200 205
Ser Tyr Phe Thr Pro Thr Phe Trp Ser Asn Pro Ser Leu Ser Leu Thr
210 215 220
Phe Ala Asp Arg Lys Pro Cys Tyr Phe Asn Thr Gly Val Met Val Ile
225 230 235 240
Asp Leu Asp Arg Trp Arg Glu Gly Asp Tyr Thr Thr Lys Ile Glu Glu
245 250 255
Trp Met Glu Leu Gln Lys Arg Ile Arg Ile Tyr Glu Leu Gly Ser Leu
260 265 270
Pro Pro Phe Met Leu Val Phe Ala Gly Asp Ile Val Pro Val Asp His
275 280 285
Arg Trp Asn Gln His Gly Leu Gly Gly Asp Asn Phe Lys Gly Leu Cys
290 295 300
Arg Asp Leu His Pro Gly Pro Ala Ser Leu Leu His Trp Ser Gly Lys
305 310 315 320
Gly Lys Pro Trp Ala Arg Leu Asp Ala Asn Arg Pro Cys Pro Leu Asp
325 330 335
Ala Leu Trp Ala Pro Tyr Asp Leu Leu Gln Thr Pro Phe Ala Leu Asp
340 345 350
Cys
<210> 262
<211> 360
<212> PRT
<213> 毛果杨()
<220>
<223> 木聚糖生物合成酶PARVUS同系物 GI:224136594
<400> 262
Met Pro Arg Leu Ile Arg Lys Leu Thr Leu Leu Phe Thr Leu Leu Phe
1 5 10 15
Leu Phe Leu Ser Leu Thr Thr Asn Ala Ala Ala Thr Thr Thr Ile Thr
20 25 30
Gln Gln Phe Lys Glu Ala Pro Gln Phe Tyr Asn Ser Pro Glu Cys Pro
35 40 45
Ser Ile Asp Gln Asp Glu Ile Asp Ser Glu Ala Glu Pro Asp Gly Asp
50 55 60
Asn Thr Ile Phe Cys Ser Glu His Ala Val His Val Ala Met Thr Leu
65 70 75 80
Asp Ala Ala Tyr Ile Arg Gly Ser Met Ala Ala Ile Leu Ser Val Leu
85 90 95
Gln His Thr Ser Cys Pro Gln Asn Ile Ala Phe His Phe Val Ala Ser
100 105 110
Ala Ser Ala Asn Ala Ser Leu Leu Arg Ala Thr Ile Ser Ser Ser Phe
115 120 125
Pro Tyr Leu Lys Phe Arg Ala Tyr Thr Phe Asp Asp Ser Ser Val Ser
130 135 140
Gly Leu Ile Ser Thr Ser Ile Arg Ser Ala Leu Asp Cys Pro Leu Asn
145 150 155 160
Tyr Ala Arg Ser Tyr Leu Ala Asn Ile Leu Pro Leu Cys Val Arg Arg
165 170 175
Val Val Tyr Leu Asp Ser Asp Leu Val Leu Val Asp Asp Ile Ala Lys
180 185 190
Leu Ala Ala Thr Pro Leu Gly Glu Lys Ser Val Leu Ala Ala Pro Glu
195 200 205
Tyr Cys Asn Ala Asn Phe Thr Ser Tyr Phe Thr Pro Thr Phe Trp Ser
210 215 220
Asn Pro Ser Leu Ser Leu Thr Phe Ala Asp Arg Arg Pro Cys Tyr Phe
225 230 235 240
Asn Thr Gly Val Met Val Ile Asp Leu Asp Arg Trp Arg Glu Gly Asp
245 250 255
Tyr Thr Thr Lys Ile Glu Glu Trp Met Glu Leu Gln Lys Arg Met Arg
260 265 270
Ile Tyr Glu Leu Gly Ser Leu Pro Pro Phe Leu Leu Val Phe Ala Gly
275 280 285
Asp Ile Val Pro Val Asp His Lys Trp Asn Gln His Gly Leu Gly Gly
290 295 300
Asp Asn Phe Arg Gly Leu Cys Arg Asp Leu His Pro Gly Pro Val Ser
305 310 315 320
Leu Leu His Trp Ser Gly Lys Gly Lys Pro Trp Ala Arg Leu Asp Ala
325 330 335
Asn Arg Pro Cys Pro Leu Asp Ala Leu Trp Ala Pro Tyr Asp Leu Leu
340 345 350
Gln Thr Pro Phe Ala Leu Asp Ser
355 360
<210> 263
<211> 352
<212> PRT
<213> 毛果杨()
<220>
<223> 木聚糖生物合成酶PARVUS同系物 GI:224096902
<400> 263
Met Phe Lys Leu Gly Leu Lys Ser Pro Pro Pro Ser Ser Ser Pro His
1 5 10 15
His Leu Leu Pro Ile Leu Leu Leu Ser Ile Ile Ile Ile Asn Ser Thr
20 25 30
Ala Thr Ala Thr Ala Asn Gly Lys Gly Phe Lys Glu Ala Pro Gln Phe
35 40 45
Tyr Asn Ser Pro Ser Cys Pro Ser Ile Ser Thr Gln Asp Met Cys Ser
50 55 60
Gln Gln Ala Val His Val Ala Met Thr Leu Asp Phe Pro Tyr Leu Arg
65 70 75 80
Gly Thr Met Ala Ala Ile Phe Ser Ile Leu Gln His Ser Ser Cys Pro
85 90 95
Glu Asn Ile Arg Phe His Phe Ile Ser Ser Pro Ser Ser Thr His Leu
100 105 110
His Glu Thr Ile Thr Ser Ser Phe Pro Tyr Leu Arg Ser Gln Ile Tyr
115 120 125
Ser Phe Asp Thr Asn Pro Val Ser Gly Leu Ile Ser Thr Ser Ile Arg
130 135 140
Ser Ala Leu Asp Ser Pro Leu Asn Tyr Ala Arg Asn Tyr Leu Ala Asn
145 150 155 160
Ile Leu Pro Pro Cys Val Pro Lys Val Val Tyr Leu Asp Ser Asp Leu
165 170 175
Val Leu Val Asp Asp Ile Ala Ser Leu Ala Ala Thr Pro Leu Gly Thr
180 185 190
Gly Thr Val Leu Ala Ala Pro Glu Tyr Cys Asn Ala Asn Phe Thr Thr
195 200 205
Tyr Phe Thr Pro Thr Phe Trp Ala Asn Pro Met Leu Ser Leu Thr Phe
210 215 220
Ser Gly Arg Asn Ala Cys Tyr Phe Asn Thr Gly Val Met Val Ile Asp
225 230 235 240
Leu Glu Arg Trp Arg Glu Gly Asp Tyr Thr Thr Lys Ile Val Glu Trp
245 250 255
Met Glu Leu Gln Lys Arg Met Arg Ile Tyr Glu Leu Gly Ser Leu Pro
260 265 270
Pro Phe Leu Leu Val Phe Ala Gly Asn Ile Ala Ala Val Asp His Lys
275 280 285
Trp Asn Gln His Gly Leu Gly Gly Asp Asn Phe Arg Gly Leu Cys Arg
290 295 300
Asn Leu His Pro Gly Pro Val Ser Leu Leu His Trp Ser Gly Lys Gly
305 310 315 320
Lys Pro Trp Val Arg Leu Asp Ala Asn Arg Pro Cys Pro Leu Asp Ala
325 330 335
Leu Trp Ala Pro Tyr Asp Leu Leu Arg Thr Pro Phe Ala Leu Glu Ser
340 345 350
<210> 264
<211> 352
<212> PRT
<213> 毛果杨()
<220>
<223> 木聚糖生物合成酶PARVUS同系物 GI:224133914
<400> 264
Met Tyr Lys Pro Gly Arg Lys Leu Thr Leu Pro Ser Pro Pro His Leu
1 5 10 15
Leu Leu Ser Leu Leu Leu Leu Leu Ser Ile Ile Ile Ile Asn Ala Thr
20 25 30
Thr Thr Ala Thr Val Asn Thr Lys Arg Phe Arg Glu Ala Pro Gln Phe
35 40 45
Tyr Asn Ser Pro Thr Cys Pro Ser Ile Ser Thr Lys Asp Met Cys Ser
50 55 60
Gln Gln Ala Val His Val Ala Met Thr Leu Asp Phe Pro Tyr Leu Arg
65 70 75 80
Gly Thr Met Ala Ala Ile Leu Ser Ile Leu Gln His Ser Ser Cys Pro
85 90 95
Glu Asn Thr Arg Phe His Phe Ile Ser Ser Pro Ser Ser Thr Tyr Leu
100 105 110
His Glu Thr Ile Thr Ser Ser Phe Pro Tyr Leu Arg Ser Gln Ile His
115 120 125
Pro Phe Asp Thr Asn Ser Val Ser Gly Leu Ile Ser Thr Ser Ile Arg
130 135 140
Ser Ala Leu Asp Ser Pro Leu Asn Tyr Ala Arg Asn Tyr Leu Ala Asn
145 150 155 160
Ile Leu Pro Pro Cys Val Arg Lys Ala Val Tyr Leu Asp Ser Asp Leu
165 170 175
Val Leu Val Asp Asp Ile Ala Met Leu Ala Ala Thr Pro Leu Gly Thr
180 185 190
Gly Thr Val Leu Ala Ala Pro Glu Tyr Cys Asn Ala Asn Ile Thr Ala
195 200 205
Tyr Phe Thr Pro Thr Phe Trp Ala Asn Pro Ser Leu Ser Leu Thr Phe
210 215 220
Ser Gly Arg Asn Ala Cys Tyr Phe Asn Thr Gly Val Met Ile Ile Asp
225 230 235 240
Leu Glu Arg Trp Arg Glu Gly Asp Tyr Thr Thr Lys Ile Val Glu Trp
245 250 255
Met Glu Leu Gln Lys Arg Met Arg Ile Tyr Glu Leu Gly Ser Leu Pro
260 265 270
Pro Phe Leu Leu Val Phe Ala Gly Asn Ile Ala Ala Val Asp His Lys
275 280 285
Trp Asn Gln His Gly Leu Gly Gly Asp Asn Phe Arg Gly Leu Cys Arg
290 295 300
Asp Leu His Pro Gly Pro Val Ser Leu Leu His Trp Ser Gly Lys Gly
305 310 315 320
Lys Pro Trp Val Arg Leu Asp Glu Asn Arg Pro Cys Pro Leu Asp Ala
325 330 335
Leu Trp Ala Pro Tyr Asp Leu Met Gln Thr Pro Phe Ala Ile Asp Phe
340 345 350
<210> 265
<211> 341
<212> PRT
<213> 拟南芥()
<220>
<223> 木聚糖生物合成酶PARVUS同系物 GI:79439859
<400> 265
Met His Ser Lys Phe Ile Leu Tyr Leu Ser Ile Leu Ala Val Phe Thr
1 5 10 15
Val Ser Phe Ala Gly Gly Glu Arg Phe Lys Glu Ala Pro Lys Phe Phe
20 25 30
Asn Ser Pro Glu Cys Leu Thr Ile Glu Asn Asp Glu Asp Phe Val Cys
35 40 45
Ser Asp Lys Ala Ile His Val Ala Met Thr Leu Asp Thr Ala Tyr Leu
50 55 60
Arg Gly Ser Met Ala Val Ile Leu Ser Val Leu Gln His Ser Ser Cys
65 70 75 80
Pro Gln Asn Ile Val Phe His Phe Val Thr Ser Lys Gln Ser His Arg
85 90 95
Leu Gln Asn Tyr Val Val Ala Ser Phe Pro Tyr Leu Lys Phe Arg Ile
100 105 110
Tyr Pro Tyr Asp Val Ala Ala Ile Ser Gly Leu Ile Ser Thr Ser Ile
115 120 125
Arg Ser Ala Leu Asp Ser Pro Leu Asn Tyr Ala Arg Asn Tyr Leu Ala
130 135 140
Asp Ile Leu Pro Thr Cys Leu Ser Arg Val Val Tyr Leu Asp Ser Asp
145 150 155 160
Leu Ile Leu Val Asp Asp Ile Ser Lys Leu Phe Ser Thr His Ile Pro
165 170 175
Thr Asp Val Val Leu Ala Ala Pro Glu Tyr Cys Asn Ala Asn Phe Thr
180 185 190
Thr Tyr Phe Thr Pro Thr Phe Trp Ser Asn Pro Ser Leu Ser Ile Thr
195 200 205
Leu Ser Leu Asn Arg Arg Ala Thr Pro Cys Tyr Phe Asn Thr Gly Val
210 215 220
Met Val Ile Glu Leu Lys Lys Trp Arg Glu Gly Asp Tyr Thr Arg Lys
225 230 235 240
Ile Ile Glu Trp Met Glu Leu Gln Lys Arg Ile Arg Ile Tyr Glu Leu
245 250 255
Gly Ser Leu Pro Pro Phe Leu Leu Val Phe Ala Gly Asn Ile Ala Pro
260 265 270
Val Asp His Arg Trp Asn Gln His Gly Leu Gly Gly Asp Asn Phe Arg
275 280 285
Gly Leu Cys Arg Asp Leu His Pro Gly Pro Val Ser Leu Leu His Trp
290 295 300
Ser Gly Lys Gly Lys Pro Trp Val Arg Leu Asp Asp Gly Arg Pro Cys
305 310 315 320
Pro Leu Asp Ala Leu Trp Val Pro Tyr Asp Leu Leu Glu Ser Arg Phe
325 330 335
Asp Leu Ile Glu Ser
340
<210> 266
<211> 342
<212> PRT
<213> 毛果杨()
<220>
<223> 木聚糖生物合成酶PARVUS同系物 GI:224108539
<400> 266
Met Ser Leu Leu Ser Ile Phe Leu Phe Leu Asn Ala Val Val Phe Ser
1 5 10 15
Ala His Gly Leu Ser Ser Ala Glu Leu Pro Ala Phe Arg Glu Ala Pro
20 25 30
Ala Phe Arg Asn Gly Arg Glu Cys Pro Lys Thr Thr Trp Leu Ser Ser
35 40 45
Leu Asn Asn Tyr His Asp Pro Ser Ile Ile His Ile Ala Met Thr Leu
50 55 60
Asp Ala Thr Tyr Leu Arg Gly Ser Val Ala Gly Val Leu Ser Val Leu
65 70 75 80
Gln His Ala Ala Cys Pro Glu Asn Val Val Phe His Phe Ile Ala Thr
85 90 95
His Arg Arg Ala Asp Leu Arg Arg Thr Ile Thr Ser Thr Phe Pro Tyr
100 105 110
Gln Thr Phe His Leu Tyr His Phe Asn Thr Asp Leu Val Lys Gly Lys
115 120 125
Ile Ser Ser Ser Ile Arg Arg Ala Leu Asp Gln Pro Leu Asn Tyr Ala
130 135 140
Arg Ile Tyr Leu Ala Asp Leu Leu Pro Met Ser Val Arg Arg Ile Ile
145 150 155 160
Tyr Phe Asp Ser Asp Leu Ile Leu Val Asp Asp Val Ala Lys Leu Trp
165 170 175
Asn Ile Asn Leu Gly Ala His Val Leu Gly Ala Pro Glu Tyr Cys His
180 185 190
Ala Asn Phe Thr Asn Tyr Phe Asn Ser Arg Phe Trp Ser Asn Ser Ala
195 200 205
Cys Ala Ala Ser Leu Arg Gly Arg Arg Ala Cys Tyr Phe Asn Thr Gly
210 215 220
Val Met Val Ile Asp Leu Gly Lys Trp Arg Glu Gly Lys Tyr Thr Glu
225 230 235 240
Arg Leu Glu Tyr Trp Met Lys Val Gln Lys Lys Tyr Arg Ile Tyr Glu
245 250 255
Leu Gly Ser Leu Pro Pro Phe Leu Leu Val Phe Ala Gly Asp Val Glu
260 265 270
Gly Val Gly His Arg Trp Asn Gln His Gly Leu Gly Gly Asp Asn Leu
275 280 285
Glu Gly Leu Cys Arg Asp Leu His Pro Gly Pro Val Ser Leu Leu His
290 295 300
Trp Ser Gly Lys Gly Lys Pro Trp Leu Arg Leu Asp Ser Lys Arg Pro
305 310 315 320
Cys Pro Leu Asp Tyr Leu Trp Ala Pro Tyr Asp Leu Tyr Arg His Ser
325 330 335
Ser Leu Phe Cys Asp Ser
340
<210> 267
<211> 346
<212> PRT
<213> 毛果杨()
<220>
<223> 木聚糖生物合成酶PARVUS同系物 GI:224101685
<400> 267
Met Ser Ile Leu Cys Ile Phe Leu Phe Leu Thr Thr Val Val Val Ser
1 5 10 15
Val Gln Ser Leu Pro Asn Ala Glu Leu Pro Ala Phe Arg Glu Ala Pro
20 25 30
Ala Phe Arg Asn Gly Arg Glu Cys Pro Lys Lys Thr Trp Pro Ser Ser
35 40 45
Phe Asn Asn Leu Asn His His Arg His Asp Pro Ser Ile Ile His Ile
50 55 60
Ala Met Thr Leu Asp Ala Thr Tyr Leu Arg Gly Ser Val Ala Gly Val
65 70 75 80
Leu Ser Val Leu Gln His Ala Ala Cys Pro Glu His Ile Val Phe His
85 90 95
Phe Ile Ala Thr His Arg Arg Ala Asp Leu Arg Arg Thr Ile Thr Ser
100 105 110
Thr Phe Pro Tyr Leu Thr Phe His Leu Tyr His Phe Asn Thr Asp Leu
115 120 125
Val Arg Gly Lys Ile Ser Ser Ser Ile Arg Arg Ala Leu Asp Gln Pro
130 135 140
Leu Asn Tyr Ala Arg Ile Tyr Leu Ala Asp Leu Leu Pro Phe Thr Val
145 150 155 160
Arg Arg Ile Ile Tyr Phe Asp Ser Asp Leu Ile Val Val Asp Asp Val
165 170 175
Ala Lys Leu Trp Asn Ile Asn Leu Gly Ala His Val Leu Gly Ala Pro
180 185 190
Glu Tyr Cys His Val Asn Phe Ser Tyr Tyr Phe Asn Ser Arg Phe Trp
195 200 205
Ser Ser Pro Val Tyr Ala Thr Ser Phe Thr Gly Arg Arg Ala Cys Tyr
210 215 220
Phe Asn Thr Gly Val Met Val Ile Asp Leu Arg Lys Trp Arg Glu Gly
225 230 235 240
Lys Tyr Thr Glu Lys Leu Glu Tyr Trp Met Arg Val Gln Lys Lys Asn
245 250 255
Arg Ile Tyr Glu Leu Gly Ser Leu Pro Pro Phe Leu Leu Val Phe Ala
260 265 270
Gly Asp Val Glu Gly Val Glu His Arg Trp Asn Gln His Gly Leu Gly
275 280 285
Gly Asp Asn Leu Glu Gly Leu Cys Arg Asp Leu His Pro Gly Pro Val
290 295 300
Ser Leu Leu His Trp Ser Gly Lys Gly Lys Pro Trp Leu Arg Leu Asn
305 310 315 320
Ser Lys Arg Pro Cys Pro Leu Asp Ser Leu Trp Ala Pro Tyr Asp Leu
325 330 335
Tyr Arg His Pro Thr Leu Phe Cys Asp Thr
340 345
<210> 268
<211> 360
<212> PRT
<213> 水稻()
<220>
<223> 木聚糖生物合成酶PARVUS同系物 GI:297601461
<400> 268
Met Asp Asp Met Ala Lys Leu Ala Gln Met Pro Arg Ala Pro Leu Leu
1 5 10 15
Val Leu Leu Leu Met Leu Gly Val Gly Ala Ala Val Ala Val Pro Glu
20 25 30
Tyr Arg Glu Ala Pro His Phe Thr Asn Ser Ala Ala Ala Arg Cys Pro
35 40 45
Pro Pro Leu Pro Ala Thr Asp Ala Asp Ala Ala Cys Ser Pro His Ala
50 55 60
Ala Val His Val Ala Met Thr Leu Asp Ala Pro Tyr Leu Arg Gly Thr
65 70 75 80
Met Ala Ala Val Leu Ser Val Leu Arg His Ala Ser Cys Pro Glu Ser
85 90 95
Val His Phe His Phe Leu Ala Ser Ser Ser Ser Ser Pro Glu Ala Ala
100 105 110
Ala Ala Val Arg Glu Leu Arg Asp Thr Val Arg Ala Ser Phe Pro Ser
115 120 125
Leu Ala Phe Arg Val Tyr Pro Phe Asp Glu Ser Arg Val Ala Gly Leu
130 135 140
Ile Ser Thr Ser Ile Arg Gly Ala Leu Asp Arg Pro Leu Asn Tyr Ala
145 150 155 160
Arg Ser Tyr Leu Ala Thr Thr Leu Pro Ala Cys Val Arg Arg Val Val
165 170 175
Tyr Leu Asp Ser Asp Val Val Val Thr Asp Asp Ile Ala Ala Leu Ala
180 185 190
Ala Thr Pro Leu Pro Gly Glu Ala Ala Val Ala Ala Pro Glu Tyr Cys
195 200 205
Gly Ala Asn Phe Thr Ala Tyr Phe Thr Pro Gly Phe Trp Ala Ser Arg
210 215 220
Ala Leu Ser Glu Ala Ala Phe Ala Gly Arg Arg Ala Cys Tyr Phe Asn
225 230 235 240
Thr Gly Val Met Val Leu Asp Leu Pro Arg Trp Arg Arg Ala Gly Tyr
245 250 255
Thr Ala Gln Ile Glu Glu Trp Met Glu Leu Gln Arg Arg Val Arg Ile
260 265 270
Tyr Glu Leu Gly Ser Leu Pro Pro Phe Leu Leu Val Phe Ala Gly Arg
275 280 285
Ile Ala Ala Val Asp His Arg Trp Asn Gln His Gly Leu Gly Gly Asp
290 295 300
Asn Tyr Arg Gly Leu Cys Arg Gly Leu His Ala Gly Ala Val Ser Leu
305 310 315 320
Leu His Trp Ser Gly Lys Gly Lys Pro Trp Asp Arg Leu Asp Ala Gly
325 330 335
Lys Pro Cys Pro Leu Asp Ala Val Trp Ala Lys Tyr Asp Leu Leu Arg
340 345 350
Pro Ala Ala Ala Ile Glu Thr Ser
355 360
<210> 269
<211> 346
<212> PRT
<213> 拟南芥()
<220>
<223> 木聚糖生物合成酶PARVUS同系物 GI:15235247
<400> 269
Met Leu Trp Ile Thr Arg Phe Ala Gly Leu Phe Ser Ala Ala Met Ala
1 5 10 15
Val Ile Val Leu Ser Pro Ser Leu Gln Ser Phe Pro Pro Ala Ala Ala
20 25 30
Ile Arg Ser Ser Pro Ser Pro Ile Phe Arg Lys Ala Pro Ala Val Phe
35 40 45
Asn Asn Gly Asp Glu Cys Leu Ser Ser Gly Gly Val Cys Asn Pro Ser
50 55 60
Leu Val His Val Ala Ile Thr Leu Asp Val Glu Tyr Leu Arg Gly Ser
65 70 75 80
Ile Ala Ala Val Asn Ser Ile Leu Gln His Ser Val Cys Pro Glu Ser
85 90 95
Val Phe Phe His Phe Ile Ala Val Ser Glu Glu Thr Asn Leu Leu Glu
100 105 110
Ser Leu Val Arg Ser Val Phe Pro Arg Leu Lys Phe Asn Ile Tyr Asp
115 120 125
Phe Ala Pro Glu Thr Val Arg Gly Leu Ile Ser Ser Ser Val Arg Gln
130 135 140
Ala Leu Glu Gln Pro Leu Asn Tyr Ala Arg Ser Tyr Leu Ala Asp Leu
145 150 155 160
Leu Glu Pro Cys Val Asn Arg Val Ile Tyr Leu Asp Ser Asp Leu Val
165 170 175
Val Val Asp Asp Ile Ala Lys Leu Trp Lys Thr Ser Leu Gly Ser Arg
180 185 190
Ile Ile Gly Ala Pro Glu Tyr Cys His Ala Asn Phe Thr Lys Tyr Phe
195 200 205
Thr Gly Gly Phe Trp Ser Glu Glu Arg Phe Ser Gly Thr Phe Arg Gly
210 215 220
Arg Lys Pro Cys Tyr Phe Asn Thr Gly Val Met Val Ile Asp Leu Lys
225 230 235 240
Lys Trp Arg Arg Gly Gly Tyr Thr Lys Arg Ile Glu Lys Trp Met Glu
245 250 255
Ile Gln Arg Arg Glu Arg Ile Tyr Glu Leu Gly Ser Leu Pro Pro Phe
260 265 270
Leu Leu Val Phe Ser Gly His Val Ala Pro Ile Ser His Arg Trp Asn
275 280 285
Gln His Gly Leu Gly Gly Asp Asn Val Arg Gly Ser Cys Arg Asp Leu
290 295 300
His Pro Gly Pro Val Ser Leu Leu His Trp Ser Gly Ser Gly Lys Pro
305 310 315 320
Trp Ile Arg Leu Asp Ser Lys Arg Pro Cys Pro Leu Asp Ala Leu Trp
325 330 335
Thr Pro Tyr Asp Leu Tyr Arg His Ser His
340 345
<210> 270
<211> 390
<212> PRT
<213> 拟南芥()
<220>
<223> 木聚糖生物合成酶PARVUS同系物 GI:18409445
<400> 270
Met Arg Leu Arg Phe Pro Met Lys Ser Ala Val Leu Ala Phe Ala Ile
1 5 10 15
Phe Leu Val Phe Ile Pro Leu Phe Ser Val Gly Ile Arg Met Ile Pro
20 25 30
Gly Arg Leu Thr Ala Val Ser Ala Thr Val Gly Asn Gly Phe Asp Leu
35 40 45
Gly Ser Phe Val Glu Ala Pro Glu Tyr Arg Asn Gly Lys Glu Cys Val
50 55 60
Ser Gln Ser Leu Asn Arg Glu Asn Phe Val Ser Ser Cys Asp Ala Ser
65 70 75 80
Leu Val His Val Ala Met Thr Leu Asp Ser Glu Tyr Leu Arg Gly Ser
85 90 95
Ile Ala Ala Val His Ser Met Leu Arg His Ala Ser Cys Pro Glu Asn
100 105 110
Val Phe Phe His Leu Ile Ala Ala Glu Phe Asp Pro Ala Ser Pro Arg
115 120 125
Val Leu Ser Gln Leu Val Arg Ser Thr Phe Pro Ser Leu Asn Phe Lys
130 135 140
Val Tyr Ile Phe Arg Glu Asp Thr Val Ile Asn Leu Ile Ser Ser Ser
145 150 155 160
Ile Arg Gln Ala Leu Glu Asn Pro Leu Asn Tyr Ala Arg Asn Tyr Leu
165 170 175
Gly Asp Ile Leu Asp Pro Cys Val Asp Arg Val Ile Tyr Leu Asp Ser
180 185 190
Asp Ile Ile Val Val Asp Asp Ile Thr Lys Leu Trp Asn Thr Ser Leu
195 200 205
Thr Gly Ser Arg Ile Ile Gly Ala Pro Glu Tyr Cys His Ala Asn Phe
210 215 220
Thr Lys Tyr Phe Thr Ser Gly Phe Trp Ser Asp Pro Ala Leu Pro Gly
225 230 235 240
Phe Phe Ser Gly Arg Lys Pro Cys Tyr Phe Asn Thr Gly Val Met Val
245 250 255
Met Asp Leu Val Arg Trp Arg Glu Gly Asn Tyr Arg Glu Lys Leu Glu
260 265 270
Thr Trp Met Gln Ile Gln Lys Lys Lys Arg Ile Tyr Asp Leu Gly Ser
275 280 285
Leu Pro Pro Phe Leu Leu Val Phe Ala Gly Asn Val Glu Ala Ile Asp
290 295 300
His Arg Trp Asn Gln His Gly Leu Gly Gly Asp Asn Val Arg Gly Ser
305 310 315 320
Cys Arg Ser Leu His Lys Gly Pro Val Ser Leu Leu His Trp Ser Gly
325 330 335
Lys Gly Lys Pro Trp Val Arg Leu Asp Glu Lys Arg Pro Cys Pro Leu
340 345 350
Asp His Leu Trp Glu Pro Tyr Asp Leu Tyr Glu His Lys Ile Glu Arg
355 360 365
Ala Lys Asp Gln Ser Leu Phe Gly Phe Ser Ser Leu Ser Glu Leu Thr
370 375 380
Glu Asp Ser Ser Phe Phe
385 390
<210> 271
<211> 365
<212> PRT
<213> 拟南芥()
<220>
<223> 木聚糖生物合成酶PARVUS同系物 GI:30689156
<400> 271
Met Met Ser Gly Ser Arg Leu Ala Ser Arg Leu Ile Ile Ile Phe Ser
1 5 10 15
Ile Ile Ser Thr Ser Phe Phe Thr Val Glu Ser Ile Arg Leu Phe Pro
20 25 30
Asp Ser Phe Asp Asp Ala Ser Ser Asp Leu Met Glu Ala Pro Ala Tyr
35 40 45
Gln Asn Gly Leu Asp Cys Ser Val Leu Ala Lys Asn Arg Leu Leu Leu
50 55 60
Ala Cys Asp Pro Ser Ala Val His Ile Ala Met Thr Leu Asp Pro Ala
65 70 75 80
Tyr Leu Arg Gly Thr Val Ser Ala Val His Ser Ile Leu Lys His Thr
85 90 95
Ser Cys Pro Glu Asn Ile Phe Phe His Phe Ile Ala Ser Gly Thr Ser
100 105 110
Gln Gly Ser Leu Ala Lys Thr Leu Ser Ser Val Phe Pro Ser Leu Ser
115 120 125
Phe Lys Val Tyr Thr Phe Glu Glu Thr Thr Val Lys Asn Leu Ile Ser
130 135 140
Ser Ser Ile Arg Gln Ala Leu Asp Ser Pro Leu Asn Tyr Ala Arg Ser
145 150 155 160
Tyr Leu Ser Glu Ile Leu Ser Ser Cys Val Ser Arg Val Ile Tyr Leu
165 170 175
Asp Ser Asp Val Ile Val Val Asp Asp Ile Gln Lys Leu Trp Lys Ile
180 185 190
Ser Leu Ser Gly Ser Arg Thr Ile Gly Ala Pro Glu Tyr Cys His Ala
195 200 205
Asn Phe Thr Lys Tyr Phe Thr Asp Ser Phe Trp Ser Asp Gln Lys Leu
210 215 220
Ser Ser Val Phe Asp Ser Lys Thr Pro Cys Tyr Phe Asn Thr Gly Val
225 230 235 240
Met Val Ile Asp Leu Glu Arg Trp Arg Glu Gly Asp Tyr Thr Arg Lys
245 250 255
Ile Glu Asn Trp Met Lys Ile Gln Lys Glu Asp Lys Arg Ile Tyr Glu
260 265 270
Leu Gly Ser Leu Pro Pro Phe Leu Leu Val Phe Gly Gly Asp Ile Glu
275 280 285
Ala Ile Asp His Gln Trp Asn Gln His Gly Leu Gly Gly Asp Asn Ile
290 295 300
Val Ser Ser Cys Arg Ser Leu His Pro Gly Pro Val Ser Leu Ile His
305 310 315 320
Trp Ser Gly Lys Gly Lys Pro Trp Val Arg Leu Asp Asp Gly Lys Pro
325 330 335
Cys Pro Ile Asp Tyr Leu Trp Ala Pro Tyr Asp Leu His Lys Ser Gln
340 345 350
Arg Gln Tyr Leu Gln Tyr Asn Gln Glu Leu Glu Ile Leu
355 360 365
<210> 272
<211> 393
<212> PRT
<213> 拟南芥()
<220>
<223> 木聚糖生物合成酶PARVUS同系物 GI:15221684
<400> 272
Met Ser Ser Arg Phe Ser Leu Thr Val Val Cys Leu Ile Ala Leu Leu
1 5 10 15
Pro Phe Val Val Gly Ile Arg Leu Ile Pro Ala Arg Ile Thr Ser Val
20 25 30
Gly Asp Gly Gly Gly Gly Gly Gly Asn Asn Gly Phe Ser Lys Leu Gly
35 40 45
Pro Phe Met Glu Ala Pro Glu Tyr Arg Asn Gly Lys Glu Cys Val Ser
50 55 60
Ser Ser Val Asn Arg Glu Asn Phe Val Ser Ser Ser Ser Ser Ser Asn
65 70 75 80
Asp Pro Ser Leu Val His Ile Ala Met Thr Leu Asp Ser Glu Tyr Leu
85 90 95
Arg Gly Ser Ile Ala Ala Val His Ser Val Leu Arg His Ala Ser Cys
100 105 110
Pro Glu Asn Val Phe Phe His Phe Ile Ala Ala Glu Phe Asp Ser Ala
115 120 125
Ser Pro Arg Val Leu Ser Gln Leu Val Arg Ser Thr Phe Pro Ser Leu
130 135 140
Asn Phe Lys Val Tyr Ile Phe Arg Glu Asp Thr Val Ile Asn Leu Ile
145 150 155 160
Ser Ser Ser Ile Arg Leu Ala Leu Glu Asn Pro Leu Asn Tyr Ala Arg
165 170 175
Asn Tyr Leu Gly Asp Ile Leu Asp Arg Ser Val Glu Arg Val Ile Tyr
180 185 190
Leu Asp Ser Asp Val Ile Thr Val Asp Asp Ile Thr Lys Leu Trp Asn
195 200 205
Thr Val Leu Thr Gly Ser Arg Val Ile Gly Ala Pro Glu Tyr Cys His
210 215 220
Ala Asn Phe Thr Gln Tyr Phe Thr Ser Gly Phe Trp Ser Asp Pro Ala
225 230 235 240
Leu Pro Gly Leu Ile Ser Gly Gln Lys Pro Cys Tyr Phe Asn Thr Gly
245 250 255
Val Met Val Met Asp Leu Val Arg Trp Arg Glu Gly Asn Tyr Arg Glu
260 265 270
Lys Leu Glu Gln Trp Met Gln Leu Gln Lys Lys Met Arg Ile Tyr Asp
275 280 285
Leu Gly Ser Leu Pro Pro Phe Leu Leu Val Phe Ala Gly Asn Val Glu
290 295 300
Ala Ile Asp His Arg Trp Asn Gln His Gly Leu Gly Gly Asp Asn Ile
305 310 315 320
Arg Gly Ser Cys Arg Ser Leu His Pro Gly Pro Val Ser Leu Leu His
325 330 335
Trp Ser Gly Lys Gly Lys Pro Trp Val Arg Leu Asp Glu Lys Arg Pro
340 345 350
Cys Pro Leu Asp His Leu Trp Glu Pro Tyr Asp Leu Tyr Lys His Lys
355 360 365
Ile Glu Arg Ala Lys Asp Gln Ser Leu Leu Gly Phe Ala Ser Leu Ser
370 375 380
Glu Leu Thr Asp Asp Ser Ser Phe Leu
385 390
<210> 273
<211> 351
<212> PRT
<213> 拟南芥()
<220>
<223> 木聚糖生物合成酶PARVUS同系物 GI:15230679
<400> 273
Met Ala Ser Arg Ser Leu Ser Tyr Thr Gln Leu Leu Gly Leu Leu Ser
1 5 10 15
Phe Ile Leu Leu Leu Val Thr Thr Thr Thr Met Ala Val Arg Val Gly
20 25 30
Val Ile Leu His Lys Pro Ser Ala Pro Thr Leu Pro Val Phe Arg Glu
35 40 45
Ala Pro Ala Phe Arg Asn Gly Asp Gln Cys Gly Thr Arg Glu Ala Asp
50 55 60
Gln Ile His Ile Ala Met Thr Leu Asp Thr Asn Tyr Leu Arg Gly Thr
65 70 75 80
Met Ala Ala Val Leu Ser Leu Leu Gln His Ser Thr Cys Pro Glu Asn
85 90 95
Leu Ser Phe His Phe Leu Ser Leu Pro His Phe Glu Asn Asp Leu Phe
100 105 110
Thr Ser Ile Lys Ser Thr Phe Pro Tyr Leu Asn Phe Lys Ile Tyr Gln
115 120 125
Phe Asp Pro Asn Leu Val Arg Ser Lys Ile Ser Lys Ser Ile Arg Gln
130 135 140
Ala Leu Asp Gln Pro Leu Asn Tyr Ala Arg Ile Tyr Leu Ala Asp Ile
145 150 155 160
Ile Pro Ser Ser Val Asp Arg Ile Ile Tyr Leu Asp Ser Asp Leu Val
165 170 175
Val Val Asp Asp Ile Glu Lys Leu Trp His Val Glu Met Glu Gly Lys
180 185 190
Val Val Ala Ala Pro Glu Tyr Cys His Ala Asn Phe Thr His Tyr Phe
195 200 205
Thr Arg Thr Phe Trp Ser Asp Pro Val Leu Val Lys Val Leu Glu Gly
210 215 220
Lys Arg Pro Cys Tyr Phe Asn Thr Gly Val Met Val Val Asp Val Asn
225 230 235 240
Lys Trp Arg Lys Gly Met Tyr Thr Gln Lys Val Glu Glu Trp Met Thr
245 250 255
Ile Gln Lys Gln Lys Arg Ile Tyr His Leu Gly Ser Leu Pro Pro Phe
260 265 270
Leu Leu Ile Phe Ala Gly Asp Ile Lys Ala Val Asn His Arg Trp Asn
275 280 285
Gln His Gly Leu Gly Gly Asp Asn Phe Glu Gly Arg Cys Arg Thr Leu
290 295 300
His Pro Gly Pro Ile Ser Leu Leu His Trp Ser Gly Lys Gly Lys Pro
305 310 315 320
Trp Leu Arg Leu Asp Ser Arg Lys Pro Cys Ile Val Asp His Leu Trp
325 330 335
Ala Pro Tyr Asp Leu Tyr Arg Ser Ser Arg His Ser Leu Glu Glu
340 345 350
<210> 274
<211> 341
<212> PRT
<213> 水稻()
<220>
<223> 木聚糖生物合成酶PARVUS同系物 GI:115459586
<400> 274
Met Arg Val Leu Ala Val Ala Leu Leu Ala Ala Ala Val Leu Ala Ala
1 5 10 15
Glu Ala Ala Ala Glu Leu Pro Glu Phe Arg Glu Ala Pro Ala Phe Arg
20 25 30
Asn Gly Ala Gly Cys Ala Gly Ala Pro Thr Ile His Ile Ala Met Thr
35 40 45
Leu Asp Thr Thr Tyr Leu Arg Gly Ser Leu Ala Gly Val Leu Ser Val
50 55 60
Leu Arg His Ala Ala Cys Pro Glu Ser Ile Ala Phe His Phe Val Ala
65 70 75 80
Ser Ser Ala Ser Pro Ala Arg Arg Leu Ala Ala Leu Arg Arg Ala Leu
85 90 95
Ala Ala Ala Phe Pro Thr Leu Pro Ala Thr Val His Arg Phe Asp Ala
100 105 110
Arg Leu Val Arg Gly Lys Ile Ser Thr Ser Val Arg Arg Ala Leu Asp
115 120 125
Gln Pro Leu Asn Tyr Ala Arg Ile Tyr Leu Ala Asp Leu Leu Pro Arg
130 135 140
Ser Val Ser Arg Val Leu Tyr Leu Asp Ser Asp Leu Leu Val Val Asp
145 150 155 160
Asp Val Ala Arg Leu Trp Ala Thr Asp Leu Gly Pro Asp Ala Ala Leu
165 170 175
Ala Ala Pro Glu Tyr Cys His Ala Asn Phe Thr Ser Tyr Phe Thr Asp
180 185 190
Ala Phe Trp Ser His Pro Glu Tyr Ser Ser Ile Phe Thr Asn Arg Gly
195 200 205
Arg Ala Pro Cys Tyr Phe Asn Thr Gly Val Met Val Ile Asp Leu Asp
210 215 220
Arg Trp Arg Ala Gly Gly Tyr Thr Val Lys Leu Glu Tyr Trp Met Glu
225 230 235 240
Val Gln Lys Gln Glu Ala Arg Ile Tyr Glu Leu Gly Ser Leu Pro Pro
245 250 255
Phe Leu Leu Val Phe Ala Gly Glu Val Lys Ala Val Glu His Arg Trp
260 265 270
Asn Gln His Gly Leu Gly Gly Asp Asn Val Ala Gly Gln Cys Arg Glu
275 280 285
Leu His Pro Gly Pro Val Ser Leu Leu His Trp Ser Gly Lys Gly Lys
290 295 300
Pro Trp Leu Arg Leu Asp Ala Gly Arg Pro Cys Pro Leu Asp Ala Leu
305 310 315 320
Trp Ala Pro Tyr Asp Leu Leu Arg Arg Arg Gly Ala Arg Asp Asp Leu
325 330 335
Leu Ala Ala Val Ala
340
<210> 275
<211> 361
<212> PRT
<213> 拟南芥()
<220>
<223> 木聚糖生物合成酶PARVUS同系物 GI:15217851
<400> 275
Met His Trp Ile Thr Arg Phe Ser Ala Phe Phe Ser Ala Ala Leu Ala
1 5 10 15
Met Ile Leu Leu Ser Pro Ser Leu Gln Ser Phe Ser Pro Ala Ala Ala
20 25 30
Ile Arg Ser Ser His Pro Tyr Ala Asp Glu Phe Lys Pro Gln Gln Asn
35 40 45
Ser Asp Tyr Ser Ser Phe Arg Glu Ser Pro Met Phe Arg Asn Ala Glu
50 55 60
Gln Cys Arg Ser Ser Gly Glu Asp Ser Gly Val Cys Asn Pro Asn Leu
65 70 75 80
Val His Val Ala Ile Thr Leu Asp Ile Asp Tyr Leu Arg Gly Ser Ile
85 90 95
Ala Ala Val Asn Ser Ile Leu Gln His Ser Met Cys Pro Gln Ser Val
100 105 110
Phe Phe His Phe Leu Val Ser Ser Glu Ser Gln Asn Leu Glu Ser Leu
115 120 125
Ile Arg Ser Thr Phe Pro Lys Leu Thr Asn Leu Lys Ile Tyr Tyr Phe
130 135 140
Ala Pro Glu Thr Val Gln Ser Leu Ile Ser Ser Ser Val Arg Gln Ala
145 150 155 160
Leu Glu Gln Pro Leu Asn Tyr Ala Arg Asn Tyr Leu Ala Asp Leu Leu
165 170 175
Glu Pro Cys Val Lys Arg Val Ile Tyr Leu Asp Ser Asp Leu Val Val
180 185 190
Val Asp Asp Ile Val Lys Leu Trp Lys Thr Gly Leu Gly Gln Arg Thr
195 200 205
Ile Gly Ala Pro Glu Tyr Cys His Ala Asn Phe Thr Lys Tyr Phe Thr
210 215 220
Gly Gly Phe Trp Ser Asp Lys Arg Phe Asn Gly Thr Phe Lys Gly Arg
225 230 235 240
Asn Pro Cys Tyr Phe Asn Thr Gly Val Met Val Ile Asp Leu Lys Lys
245 250 255
Trp Arg Gln Phe Arg Phe Thr Lys Arg Ile Glu Lys Trp Met Glu Ile
260 265 270
Gln Lys Ile Glu Arg Ile Tyr Glu Leu Gly Ser Leu Pro Pro Phe Leu
275 280 285
Leu Val Phe Ala Gly His Val Ala Pro Ile Ser His Arg Trp Asn Gln
290 295 300
His Gly Leu Gly Gly Asp Asn Val Arg Gly Ser Cys Arg Asp Leu His
305 310 315 320
Ser Gly Pro Val Ser Leu Leu His Trp Ser Gly Ser Gly Lys Pro Trp
325 330 335
Leu Arg Leu Asp Ser Lys Leu Pro Cys Pro Leu Asp Thr Leu Trp Ala
340 345 350
Pro Tyr Asp Leu Tyr Lys His Ser His
355 360
<210> 276
<211> 361
<212> PRT
<213> 拟南芥()
<220>
<223> 木聚糖生物合成酶PARVUS同系物 GI:22331906
<400> 276
Met Leu Trp Ile Met Arg Phe Ser Gly Leu Phe Ser Ala Ala Leu Val
1 5 10 15
Ile Ile Val Leu Ser Pro Ser Leu Gln Ser Phe Pro Pro Ala Glu Ala
20 25 30
Ile Arg Ser Ser His Leu Asp Ala Tyr Leu Arg Phe Pro Ser Ser Asp
35 40 45
Pro Pro Pro His Arg Phe Ser Phe Arg Lys Ala Pro Val Phe Arg Asn
50 55 60
Ala Ala Asp Cys Ala Ala Ala Asp Ile Asp Ser Gly Val Cys Asn Pro
65 70 75 80
Ser Leu Val His Val Ala Ile Thr Leu Asp Phe Glu Tyr Leu Arg Gly
85 90 95
Ser Ile Ala Ala Val His Ser Ile Leu Lys His Ser Ser Cys Pro Glu
100 105 110
Ser Val Phe Phe His Phe Leu Val Ser Glu Thr Asp Leu Glu Ser Leu
115 120 125
Ile Arg Ser Thr Phe Pro Glu Leu Lys Leu Lys Val Tyr Tyr Phe Asp
130 135 140
Pro Glu Ile Val Arg Thr Leu Ile Ser Thr Ser Val Arg Gln Ala Leu
145 150 155 160
Glu Gln Pro Leu Asn Tyr Ala Arg Asn Tyr Leu Ala Asp Leu Leu Glu
165 170 175
Pro Cys Val Arg Arg Val Ile Tyr Leu Asp Ser Asp Leu Ile Val Val
180 185 190
Asp Asp Ile Ala Lys Leu Trp Met Thr Lys Leu Gly Ser Lys Thr Ile
195 200 205
Gly Ala Pro Glu Tyr Cys His Ala Asn Phe Thr Lys Tyr Phe Thr Pro
210 215 220
Ala Phe Trp Ser Asp Glu Arg Phe Ser Gly Ala Phe Ser Gly Arg Lys
225 230 235 240
Pro Cys Tyr Phe Asn Thr Gly Val Met Val Met Asp Leu Glu Arg Trp
245 250 255
Arg Arg Val Gly Tyr Thr Glu Val Ile Glu Lys Trp Met Glu Ile Gln
260 265 270
Lys Ser Asp Arg Ile Tyr Glu Leu Gly Ser Leu Pro Pro Phe Leu Leu
275 280 285
Val Phe Ala Gly Glu Val Ala Pro Ile Glu His Arg Trp Asn Gln His
290 295 300
Gly Leu Gly Gly Asp Asn Val Arg Gly Ser Cys Arg Asp Leu His Pro
305 310 315 320
Gly Pro Val Ser Leu Leu His Trp Ser Gly Ser Gly Lys Pro Trp Phe
325 330 335
Arg Leu Asp Ser Arg Arg Pro Cys Pro Leu Asp Thr Leu Trp Ala Pro
340 345 350
Tyr Asp Leu Tyr Gly His Tyr Ser Arg
355 360
<210> 277
<211> 345
<212> PRT
<213> 拟南芥()
<220>
<223> 木聚糖生物合成酶PARVUS同系物 GI:18391493
<400> 277
Met Ser Ser Leu Arg Leu Arg Leu Cys Leu Leu Leu Leu Leu Pro Ile
1 5 10 15
Thr Ile Ser Cys Val Thr Val Thr Leu Thr Asp Leu Pro Ala Phe Arg
20 25 30
Glu Ala Pro Ala Phe Arg Asn Gly Arg Glu Cys Ser Lys Thr Thr Trp
35 40 45
Ile Pro Ser Asp His Glu His Asn Pro Ser Ile Ile His Ile Ala Met
50 55 60
Thr Leu Asp Ala Ile Tyr Leu Arg Gly Ser Val Ala Gly Val Phe Ser
65 70 75 80
Val Leu Gln His Ala Ser Cys Pro Glu Asn Ile Val Phe His Phe Ile
85 90 95
Ala Thr His Arg Arg Ser Ala Asp Leu Arg Arg Ile Ile Ser Ser Thr
100 105 110
Phe Pro Tyr Leu Thr Tyr His Ile Tyr His Phe Asp Pro Asn Leu Val
115 120 125
Arg Ser Lys Ile Ser Ser Ser Ile Arg Arg Ala Leu Asp Gln Pro Leu
130 135 140
Asn Tyr Ala Arg Ile Tyr Leu Ala Asp Leu Leu Pro Ile Ala Val Arg
145 150 155 160
Arg Val Ile Tyr Phe Asp Ser Asp Leu Val Val Val Asp Asp Val Ala
165 170 175
Lys Leu Trp Arg Ile Asp Leu Arg Arg His Val Val Gly Ala Pro Glu
180 185 190
Tyr Cys His Ala Asn Phe Thr Asn Tyr Phe Thr Ser Arg Phe Trp Ser
195 200 205
Ser Gln Gly Tyr Lys Ser Ala Leu Lys Asp Arg Lys Pro Cys Tyr Phe
210 215 220
Asn Thr Gly Val Met Val Ile Asp Leu Gly Lys Trp Arg Glu Arg Arg
225 230 235 240
Val Thr Val Lys Leu Glu Thr Trp Met Arg Ile Gln Lys Arg His Arg
245 250 255
Ile Tyr Glu Leu Gly Ser Leu Pro Pro Phe Leu Leu Val Phe Ala Gly
260 265 270
Asp Val Glu Pro Val Glu His Arg Trp Asn Gln His Gly Leu Gly Gly
275 280 285
Asp Asn Leu Glu Gly Leu Cys Arg Asn Leu His Pro Gly Pro Val Ser
290 295 300
Leu Leu His Trp Ser Gly Lys Gly Lys Pro Trp Leu Arg Leu Asp Ser
305 310 315 320
Arg Arg Pro Cys Pro Leu Asp Ser Leu Trp Ala Pro Tyr Asp Leu Phe
325 330 335
Arg Tyr Ser Pro Leu Ile Ser Asp Ser
340 345
<210> 278
<211> 347
<212> PRT
<213> 水稻()
<220>
<223> 木聚糖生物合成酶PARVUS同系物 GI:115453101
<400> 278
Met Ala Arg Cys Gly Ser Ala Ile Ala Ala Val Ala Ala Leu Leu Val
1 5 10 15
Leu Leu Gly Gly His Ala Ala Ala Arg Ile Arg Val Glu His Ser Gly
20 25 30
Met Val Ile Arg Arg Pro Ser Ser Ser Ile Pro Ser Phe Arg Glu Ala
35 40 45
Pro Ala Phe Arg Asn Gly Glu Glu Cys Gly Gly Gly Gly Arg Val Asp
50 55 60
Val Ala Met Thr Leu Asp Ala Asn Tyr Leu Arg Gly Thr Met Ala Gly
65 70 75 80
Val Leu Ser Ile Leu Gln His Thr Ala Cys Pro Glu Ser Val Ser Phe
85 90 95
His Phe Leu Ala Ala Gly Met Asp Ala Asp Leu Ala Ala Ala Val Arg
100 105 110
Ala Thr Phe Pro Tyr Leu Asp Leu Arg Val Tyr Arg Phe Asp Pro Ser
115 120 125
Arg Val Arg Gly Arg Ile Ser Arg Ser Ile Arg His Ala Leu Asp Gln
130 135 140
Pro Leu Asn Tyr Ala Arg Ile Tyr Leu Ala Asp Thr Leu Pro Pro Asp
145 150 155 160
Val Arg Arg Val Ile Tyr Leu Asp Ser Asp Val Val Val Val Asp Asp
165 170 175
Ile Arg Ala Leu Ala Ser Val Asp Leu Gly Gly His Val Val Gly Ala
180 185 190
Pro Glu Tyr Cys His Ala Asn Phe Thr Asn Tyr Phe Thr Asp Ala Phe
195 200 205
Trp Ser Asp Pro Ala Leu Asn Gly Thr Phe Ala Gly Arg Arg Pro Cys
210 215 220
Tyr Phe Asn Thr Gly Val Met Val Met Asp Val Gly Lys Trp Arg Ala
225 230 235 240
Gly Gly Tyr Thr Arg Arg Val Glu Arg Trp Met Glu Val Gln Lys Gln
245 250 255
Thr Arg Ile Tyr His Leu Gly Ser Leu Pro Pro Phe Leu Leu Val Leu
260 265 270
Ala Gly Asp Ile Gln Ala Val Asp His Arg Trp Asn Gln His Gly Leu
275 280 285
Gly Gly Asp Asn Val Lys Gly Arg Cys Arg Gly Leu His Pro Gly Pro
290 295 300
Ile Ser Leu Leu His Trp Ser Gly Lys Gly Lys Pro Trp Ile Arg Leu
305 310 315 320
Asp Ala Arg Arg Pro Cys Ala Val Asp Tyr Leu Trp Ala Pro Tyr Asp
325 330 335
Leu Phe Arg Pro Ser Ser Pro Val Leu Glu Glu
340 345
<210> 279
<211> 368
<212> PRT
<213> 水稻()
<220>
<223> 木聚糖生物合成酶PARVUS同系物 GI:115452521
<400> 279
Met Leu Trp Ala Ala Arg Val Ser Gly Phe Phe Ser Ala Ala Met Val
1 5 10 15
Met Val Val Leu Ser Pro Ser Leu Gln Ser Phe Pro Pro Ala Glu Ala
20 25 30
Ile Arg Ser Ser Gln Phe Asp Ser His Val Arg Phe Pro Gly Gln Ile
35 40 45
Ala Gly Gly Ala Arg Gly Leu Ala Phe Arg Arg Ala Pro Ala Phe Arg
50 55 60
Asn Ala Ala Asp Cys Gly Asn Ala Thr Gly Asn Val Cys Asp Pro Ser
65 70 75 80
Leu Val His Ile Ala Ile Thr Leu Asp Glu Glu Tyr Leu Arg Gly Ser
85 90 95
Val Ala Ala Val His Ser Val Val Gln His Ala Thr Cys Pro Glu Ser
100 105 110
Val Phe Phe His Phe Leu Val Ser Asp Pro Ala Leu Gly Asp Leu Val
115 120 125
Arg Ala Val Phe Pro Gln Leu Gln Phe Lys Val Tyr Tyr Phe Asp Pro
130 135 140
Asp Arg Val Arg Gly Leu Ile Ser Thr Ser Val Arg Gln Ala Leu Glu
145 150 155 160
Gln Pro Leu Asn Tyr Ala Arg Asn Tyr Leu Ala Asp Leu Leu Glu Pro
165 170 175
Cys Val Arg Arg Val Ile Tyr Leu Asp Ser Asp Leu Val Val Val Asp
180 185 190
Asp Val Ala Lys Leu Trp Arg Thr Asp Leu Gly Gly Arg Thr Val Gly
195 200 205
Ala Pro Glu Tyr Cys His Ala Asn Phe Thr Lys Tyr Phe Thr Asp Arg
210 215 220
Phe Trp Ser Asp Lys Gln Phe Ala Gly Thr Phe Ala Gly Arg Arg Pro
225 230 235 240
Cys Tyr Phe Asn Thr Gly Val Met Val Leu Asp Leu Ala Arg Trp Arg
245 250 255
Arg Thr Gly Tyr Thr Arg Arg Ile Glu Arg Trp Met Glu Ile Gln Lys
260 265 270
Ser Pro Ala Gly Arg Ile Tyr Glu Leu Gly Ser Leu Pro Pro Phe Leu
275 280 285
Leu Val Phe Ala Gly His Val Ala Pro Ile Glu His Arg Trp Asn Gln
290 295 300
His Gly Leu Gly Gly Asp Asn Val Phe Gly Ser Cys Arg Asp Leu His
305 310 315 320
Pro Gly Pro Val Ser Leu Leu His Trp Ser Gly Ser Gly Lys Pro Trp
325 330 335
Ala Arg Leu Gly Ala Gly Arg Pro Cys Pro Leu Asp Ala Leu Trp Ala
340 345 350
Pro Phe Asp Leu Tyr Gly Pro Ala Asp Ser Ala Ala Glu Gly Ser Arg
355 360 365
<210> 280
<211> 373
<212> PRT
<213> 水稻()
<220>
<223> 木聚糖生物合成酶PARVUS同系物 GI:115448575
<400> 280
Met Gly Gly Asn Pro Ala Met Arg Ala Gly Leu Ala Leu Val Val Val
1 5 10 15
Val Val Ala Val Val Val Gly Asp Val Gly Ala Ala Leu Pro Arg Phe
20 25 30
Ala Glu Ala Pro Glu Tyr Arg Asn Gly Glu Gly Cys Pro Ala Ala Val
35 40 45
Ala Gly Ala Gly Val Cys Asp Pro Gly Leu Val His Ile Ala Met Thr
50 55 60
Leu Asp Ala His Tyr Leu Arg Gly Ser Met Ala Ala Ile Tyr Ser Leu
65 70 75 80
Leu Lys His Ala Ser Cys Pro Glu Ser Ile Phe Phe His Phe Leu Ala
85 90 95
Ala Ala Asp Gly Gly Glu Gly Cys Gly Gly Gly Val Gly Glu Leu Arg
100 105 110
Thr Ala Val Ala Ala Ser Phe Pro Ser Leu Arg Phe Glu Ile Tyr Pro
115 120 125
Phe Arg Ala Asp Ala Val Thr Gly Leu Ile Ser Ala Ser Val Arg Ala
130 135 140
Ala Leu Glu Ala Pro Leu Asn Tyr Ala Arg Asn Tyr Leu Ala Asp Leu
145 150 155 160
Leu Pro Lys Cys Val Pro Arg Ala Ile Tyr Leu Asp Ser Asp Val Leu
165 170 175
Ala Val Asp Asp Val Arg Arg Leu Trp Glu Thr Arg Leu Pro Ala Ala
180 185 190
Ala Val Val Ala Ala Pro Glu Tyr Cys His Ala Asn Phe Ser Arg Tyr
195 200 205
Phe Thr Glu Thr Phe Trp Ser Asp Pro Gln Leu Gly Asp Arg Val Phe
210 215 220
Ala Gly Arg Arg Arg Ala Pro Cys Tyr Phe Asn Thr Gly Val Met Val
225 230 235 240
Ile Asp Leu Arg Arg Trp Arg Val Gly Asn Tyr Arg Arg Arg Ile Glu
245 250 255
Val Trp Met Glu Leu Gln Lys Glu Lys Arg Ile Tyr Glu Leu Gly Ser
260 265 270
Leu Pro Pro Phe Leu Leu Val Phe Ala Gly Glu Val Glu Ala Val Asp
275 280 285
His Arg Trp Asn Gln His Gly Leu Gly Gly Asp Asn Val Leu Gly Ser
290 295 300
Cys Arg Pro Leu His Lys Gly Pro Val Ser Leu Met His Trp Ser Gly
305 310 315 320
Lys Gly Lys Pro Trp Asp Arg Leu Asp Ala Gly Lys Pro Cys Pro Leu
325 330 335
Asp His Thr Trp Lys Ser Tyr Asp Leu Tyr Ile Ala Glu Gly Asp Ser
340 345 350
Ser Ser Ala Ser Ala Pro Phe Ala Leu Ser Ser Ser Ala Leu Pro Ala
355 360 365
Ala Ala Phe Ser Trp
370
<210> 281
<211> 374
<212> PRT
<213> 江南卷柏()
<220>
<223> 木聚糖生物合成酶PARVUS同系物 GI:302807664
<400> 281
Met Val Trp Ala Leu Leu Val Leu Leu Thr Ile Leu Leu Pro Ala Met
1 5 10 15
His Ser Trp Ala Pro Ala Ala Glu Ala Ile Arg Ser Ser Gln Phe Gly
20 25 30
Gln Gln Gln Leu Phe His Glu Ala Pro Ala Phe Arg Asn Gly Lys Glu
35 40 45
Cys Pro Arg Gln Arg Leu Asp Pro Ala Gln Arg Pro Gly Trp Cys His
50 55 60
Asp Pro Gly Ala Ile His Val Ala Met Thr Leu Asp Arg Ala Tyr Leu
65 70 75 80
Arg Gly Ser Met Ala Ala Val Leu Ser Ile Val Gln His Ala Val Cys
85 90 95
Pro Glu Ser Ile Val Phe His Phe Leu Ile Ala Ser Pro Gly His Asp
100 105 110
His His Pro Glu Glu Leu Pro Met Asp Ala Leu Gln Ser Val Val Lys
115 120 125
Gln Thr Phe Pro Tyr Leu Arg Phe Lys Ala Tyr Glu Phe Gln Glu Ala
130 135 140
Leu Val Arg Gly Arg Ile Ser Ser Ser Val Arg Ser Asp Leu Glu Gln
145 150 155 160
Pro Leu Asn Tyr Ala Arg Asn Tyr Leu Ala Ala Met Leu Asp Glu Cys
165 170 175
Ile His Arg Val Ile Tyr Leu Asp Ser Asp Val Val Val Val Asp Asp
180 185 190
Ile Ala Lys Leu Trp Arg Thr Glu Leu Arg Asp Gly His Val Leu Gly
195 200 205
Ala Pro Glu Tyr Cys Ala Ala Asn Phe Thr Arg Tyr Phe Thr Pro Ala
210 215 220
Phe Trp Ser Asn Glu Thr Leu Ala Ser Thr Phe Ala Ala Arg Ser Ser
225 230 235 240
Thr Pro Cys Tyr Phe Asn Thr Gly Val Met Val Met Asp Leu Arg Ala
245 250 255
Trp Arg Arg Gly Gly Tyr Thr Ala Met Leu Glu Ala Trp Met Asp Val
260 265 270
Arg Lys Glu Ser Lys Ile Tyr Glu Leu Gly Ser Leu Pro Pro Phe Leu
275 280 285
Leu Val Phe Ala Gly Glu Val Glu Ala Ile Glu His Arg Trp Asn Gln
290 295 300
His Gly Leu Gly Gly Asp Cys Val Val Gly Ser Cys Arg Asp Leu His
305 310 315 320
Pro Gly Pro Val Ser Leu Leu His Trp Ser Gly Lys Gly Lys Pro Trp
325 330 335
Ala Arg Leu Asp Ser Gly Thr Pro Cys Pro Leu Asp Ser Leu Trp Ala
340 345 350
Pro Tyr Asp Leu Phe Arg Tyr Arg His Arg His Pro Gln Leu Ala Leu
355 360 365
Ala Leu Ser Met Val Ala
370
<210> 282
<211> 345
<212> PRT
<213> 人工序列()
<220>
<223> 合成的转录因子NAC次生壁增厚促进因子1 (NST1, ANAC043) 共有序列（大多数）
<220>
<221> 变体
<222> (27)..(.27)
<223> Xaa = Gln, Lys, His, Val, Asn, Tyr, Thr或Gly
<220>
<221> 变体
<222> (145)..(.145)
<223> Xaa = Ser, Ile, Trp, Asn, Ala, Val, Gly, His或Pro
<220>
<221> 变体
<222> (147)..(.147)
<223> Xaa = His, Asp, Gly, Asn, Gln, Ser, Lys, Val或Thr
<220>
<221> 变体
<222> (176)..(.176)
<223> Xaa = Asn, Ile, Asp, Trp, Glu, Leu, Ala, Gly, Gln或Ser
<220>
<221> 变体
<222> (183)..(.183)
<223> Xaa = Gly, Leu, Cys, Ser, Thr, Phe, Ala, His, Arg或Met
<220>
<221> 变体
<222> (186)..(.186)
<223> Xaa = Thr, Ser, Asp, Ala, Arg, His, Gly, Met, Lys, Asn或Pro
<220>
<221> 变体
<222> (219)..(.219)
<223> Xaa = Asn, Pro, Tyr, Ser, Asp, His或Arg
<220>
<221> 变体
<222> (220)..(.220)
<223> Xaa = Pro, Gly, Val, Thr, Ser, Ile, Leu或Glu
<220>
<221> 变体
<222> (224)..(.224)
<223> Xaa = Asp, Val, Pro, Glu, Thr, Ala, Leu, Lys或His
<220>
<221> 变体
<222> (225)..(.225)
<223> Xaa = Pro, Leu, Ser, Arg, Gly, Lys, Gln, Thr, Asn或Glu
<220>
<221> 变体
<222> (231)..(.231)
<223> Xaa = Asn, Ser, Thr, Phe, Asp, Lys, Gly, Arg或Ile
<220>
<221> 变体
<222> (238)..(.238)
<223> Xaa = Gln, Thr, Glu, Asp, Ala, Ile, Ser, Asn, Pro或Lys
<220>
<221> 变体
<222> (243)..(.243)
<223> Xaa = Cys, Tyr, Phe, Tyr, Asn, Ala, Trp, Asp, Pro, Arg, Ser, Leu 或Glu
<220>
<221> 变体
<222> (245)..(.245)
<223> Xaa = Val, Trp, Thr, Phe, Asn, Gln, Pro, Leu, Met, His, Asp, Arg 或Glu
<220>
<221> 变体
<222> (247)..(.247)
<223> Xaa = Ser, Ile, Phe, Leu, Asp, Ala, Met, Gly或His
<220>
<221> 变体
<222> (248)..(.248)
<223> Xaa = Pro, Asp, Ser, Leu, Asn, Thr, His, Ala, Phe, Ile或Gly
<220>
<221> 变体
<222> (250)..(.250)
<223> Xaa = Thr, Gln, Val, Arg, Gly, Asn, Ile, Ala, Tyr或Leu
<220>
<221> 变体
<222> (258)..(.258)
<223> Xaa = Pro, Gln, Met, His, Leu, Ala, Thr, Val, Tyr, Arg或Phe
<220>
<221> 变体
<222> (259)..(.259)
<223> Xaa = Val, Arg, Ser, Pro, Asn, Leu, Gly, Asp, Ala或His
<220>
<221> 变体
<222> (260)..(.260)
<223> Xaa = Cys, Gly, Ser, Pro, Asn, Leu, Arg, Asp, Ala或His
<220>
<221> 变体
<222> (261)..(.261)
<223> Xaa = Asn, Lys, Tyr, Ser, Gly, Thr, Ala, Ile, Leu, Val或Phe
<220>
<221> 变体
<222> (262)..(.262)
<223> Xaa = Val, Cys, Gln, Pro, His, Asp, Ile, Gly, Thr, Met, Arg 或Ser
<220>
<221> 变体
<222> (265)..(.265)
<223> Xaa = Thr, Pro, Met, Ser, Ala, Gly或His
<220>
<221> 变体
<222> (268)..(.268)
<223> Xaa = Val, Ile, Leu, Glu, Asp, Asp, Asn, Thr, Ile, Met, His 或Ser
<220>
<221> 变体
<222> (270)..(.270)
<223> Xaa = Ser, Asn, Thr, Asp, Gly, Ala或Leu
<220>
<221> 变体
<222> (285)..(.285)
<223> Xaa = Pro, Thr, His, Asn, Gln, Gly, Ala, Leu或Val
<220>
<221> 变体
<222> (286)..(.286)
<223> Xaa = Thr, Asn, Asp, His, Gly, Pro, Cys, Ser, Glu或Arg
<220>
<221> 变体
<222> (289)..(.289)
<223> Xaa = Gly, Asn, Ser, Glu或Asp
<220>
<221> 变体
<222> (305)..(.305)
<223> Xaa = His, Glu, Ser, Asp, Pro, Val, Gly, Tyr, Phe, Ala, Met 或Arg
<220>
<221> 变体
<222> (319)..(.319)
<223> Xaa = Ser, Leu, Cys, Asn, Thr, Asp, Val, Phe, Lys或Pro
<220>
<221> 变体
<222> (320)..(.320)
<223> Xaa = Tyr, Cys, His, Asn, Arg, Gln, Asp, Ser, Gly或Phe
<220>
<221> 变体
<222> (322)..(.322)
<223> Xaa = Ala, Thr, Asn, Tyr, Trp, Pro, Ser, Met, Arg或Val
<220>
<221> 变体
<222> (323)..(.323)
<223> Xaa = Gly, His, Ser, Ala, Arg, Thr, Glu或Asp
<220>
<221> 变体
<222> (324)..(.324)
<223> Xaa = Leu, Gly, Pro, Arg, Phe, Ser, Asn或His
<220>
<221> 变体
<222> (326)..(.326)
<223> Xaa = Gln, Pro, Asn, Arg, Ala, Val, Gly, Ser或His
<220>
<221> 变体
<222> (327)..(.327)
<223> Xaa = Glu, Asp, His, Gln, Ser, Ala, Phe或Tyr
<220>
<221> 变体
<222> (331)..(.331)
<223> Xaa = Pro, Asp, Thr, Asn, Ser, Lys, Ala, Gly, Val, Cys或Gln
<220>
<221> 变体
<222> (339)..(.339)
<223> Xaa = Thr, Ala, Gly, Arg, Val或Leu
<220>
<221> 变体
<222> (340)..(.340)
<223> Xaa = Thr, Asp, Glu, Arg, Lys, Leu或Ser
<220>
<221> UNSURE
<222> (27)..(27)
<223> The 'Xaa' at location 27 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (145)..(145)
<223> The 'Xaa' at location 145 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (147)..(147)
<223> The 'Xaa' at location 147 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (176)..(176)
<223> The 'Xaa' at location 176 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (183)..(183)
<223> The 'Xaa' at location 183 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (186)..(186)
<223> The 'Xaa' at location 186 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (219)..(219)
<223> The 'Xaa' at location 219 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (220)..(220)
<223> The 'Xaa' at location 220 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (224)..(224)
<223> The 'Xaa' at location 224 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (225)..(225)
<223> The 'Xaa' at location 225 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (231)..(231)
<223> The 'Xaa' at location 231 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (238)..(238)
<223> The 'Xaa' at location 238 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (243)..(243)
<223> The 'Xaa' at location 243 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (245)..(245)
<223> The 'Xaa' at location 245 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (247)..(247)
<223> The 'Xaa' at location 247 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (248)..(248)
<223> The 'Xaa' at location 248 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (250)..(250)
<223> The 'Xaa' at location 250 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (258)..(258)
<223> The 'Xaa' at location 258 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (259)..(259)
<223> The 'Xaa' at location 259 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (260)..(260)
<223> The 'Xaa' at location 260 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (261)..(261)
<223> The 'Xaa' at location 261 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (262)..(262)
<223> The 'Xaa' at location 262 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (265)..(265)
<223> The 'Xaa' at location 265 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (268)..(268)
<223> The 'Xaa' at location 268 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (270)..(270)
<223> The 'Xaa' at location 270 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (285)..(285)
<223> The 'Xaa' at location 285 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (286)..(286)
<223> The 'Xaa' at location 286 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (289)..(289)
<223> The 'Xaa' at location 289 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (305)..(305)
<223> The 'Xaa' at location 305 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (319)..(319)
<223> The 'Xaa' at location 319 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (320)..(320)
<223> The 'Xaa' at location 320 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (322)..(322)
<223> The 'Xaa' at location 322 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (323)..(323)
<223> The 'Xaa' at location 323 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (324)..(324)
<223> The 'Xaa' at location 324 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (326)..(326)
<223> The 'Xaa' at location 326 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (327)..(327)
<223> The 'Xaa' at location 327 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (331)..(331)
<223> The 'Xaa' at location 331 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (339)..(339)
<223> The 'Xaa' at location 339 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (340)..(340)
<223> The 'Xaa' at location 340 stands for Gln, Arg, Pro, or Leu.
<400> 282
Met Met Ser Ile Ser Val Asn Gly Gln Ser Gln Val Pro Pro Gly Phe
1 5 10 15
Arg Phe His Pro Thr Glu Glu Glu Leu Leu Xaa Tyr Tyr Leu Arg Lys
20 25 30
Lys Val Ala Ser Glu Lys Ile Asp Leu Asp Val Ile Arg Asp Val Asp
35 40 45
Leu Asn Lys Leu Glu Pro Trp Asp Ile Gln Glu Lys Cys Lys Ile Gly
50 55 60
Ser Thr Pro Gln Asn Asp Trp Tyr Phe Phe Ser His Lys Asp Lys Lys
65 70 75 80
Tyr Pro Thr Gly Thr Arg Thr Asn Arg Ala Thr Ala Ala Gly Phe Trp
85 90 95
Lys Ala Thr Gly Arg Asp Lys Ala Ile Tyr Ser Ser Gly Lys Arg Ile
100 105 110
Gly Met Arg Lys Thr Leu Val Phe Tyr Lys Gly Arg Ala Pro His Gly
115 120 125
Gln Lys Ser Asp Trp Ile Met His Glu Tyr Arg Leu Asp Asp Asn Thr
130 135 140
Xaa Thr Xaa Val Ser Asn Val Ala Gly Ala Ala Gln Glu Glu Gly Trp
145 150 155 160
Val Val Cys Arg Val Phe Lys Lys Lys Asn Leu Val Lys Thr Leu Xaa
165 170 175
Ser Ser Ser Ser Ser Ser Xaa Ala Asp Xaa Arg Lys Gln Leu Ala Ala
180 185 190
Ser Cys Asn Glu Gly Ser Leu Asp Gln Ile Leu Glu Tyr Met Gly Arg
195 200 205
Thr Cys Lys Glu Glu Asn Arg Pro His Ser Xaa Xaa Asp His Ile Xaa
210 215 220
Xaa Phe Met Lys Leu Pro Xaa Leu Glu Ser Pro Asn Ser Xaa Ser Ile
225 230 235 240
Gln Asn Xaa Leu Xaa Pro Xaa Xaa Glu Xaa Glu Ala Ala Ile Thr Asn
245 250 255
Gln Xaa Xaa Xaa Xaa Xaa Leu Asp Xaa Lys Phe Xaa Asn Xaa Trp Ala
260 265 270
Ala Leu Asp Arg Leu Val Ala Ser Gln Leu Asn Gly Xaa Xaa Glu Tyr
275 280 285
Xaa Ala Ser Gly Ile Ala Asp Trp Ser Pro Leu Asp Arg Leu Ala Ala
290 295 300
Xaa Leu Leu Leu Ser Pro Asp Ser Ser Gly Gln Leu Ser Ala Xaa Xaa
305 310 315 320
Ser Xaa Xaa Xaa Thr Xaa Xaa Tyr Ala Ser Xaa Glu Gly Asp Leu Trp
325 330 335
Ser Leu Xaa Xaa Ser Ser Ser Leu Asn
340 345
<210> 283
<211> 334
<212> PRT
<213> 拟南芥()
<220>
<223> 转录因子NAC次生壁增厚促进因子2 (NST2, AtNST2, ANAC066)
<400> 283
Met Asn Ile Ser Val Asn Gly Gln Ser Gln Val Pro Pro Gly Phe Arg
1 5 10 15
Phe His Pro Thr Glu Glu Glu Leu Leu Lys Tyr Tyr Leu Arg Lys Lys
20 25 30
Ile Ser Asn Ile Lys Ile Asp Leu Asp Val Ile Pro Asp Ile Asp Leu
35 40 45
Asn Lys Leu Glu Pro Trp Asp Ile Gln Glu Met Cys Lys Ile Gly Thr
50 55 60
Thr Pro Gln Asn Asp Trp Tyr Phe Tyr Ser His Lys Asp Lys Lys Tyr
65 70 75 80
Pro Thr Gly Thr Arg Thr Asn Arg Ala Thr Thr Val Gly Phe Trp Lys
85 90 95
Ala Thr Gly Arg Asp Lys Thr Ile Tyr Thr Asn Gly Asp Arg Ile Gly
100 105 110
Met Arg Lys Thr Leu Val Phe Tyr Lys Gly Arg Ala Pro His Gly Gln
115 120 125
Lys Ser Asp Trp Ile Met His Glu Tyr Arg Leu Asp Glu Ser Val Leu
130 135 140
Ile Ser Ser Cys Gly Asp His Asp Val Asn Val Glu Thr Cys Asp Val
145 150 155 160
Ile Gly Ser Asp Glu Gly Trp Val Val Cys Arg Val Phe Lys Lys Asn
165 170 175
Asn Leu Cys Lys Asn Met Ile Ser Ser Ser Pro Ala Ser Ser Val Lys
180 185 190
Thr Pro Ser Phe Asn Glu Glu Thr Ile Glu Gln Leu Leu Glu Val Met
195 200 205
Gly Gln Ser Cys Lys Gly Glu Ile Val Leu Asp Pro Phe Leu Lys Leu
210 215 220
Pro Asn Leu Glu Cys His Asn Asn Thr Thr Ile Thr Ser Tyr Gln Trp
225 230 235 240
Leu Ile Asp Asp Gln Val Asn Asn Cys His Val Ser Lys Val Met Asp
245 250 255
Pro Ser Phe Ile Thr Ser Trp Ala Ala Leu Asp Arg Leu Val Ala Ser
260 265 270
Gln Leu Asn Gly Pro Asn Ser Tyr Ser Ile Pro Ala Val Asn Glu Thr
275 280 285
Ser Gln Ser Pro Tyr His Gly Leu Asn Arg Ser Gly Cys Asn Thr Gly
290 295 300
Leu Thr Pro Asp Tyr Tyr Ile Pro Glu Ile Asp Leu Trp Asn Glu Ala
305 310 315 320
Asp Phe Ala Arg Thr Thr Cys His Leu Leu Asn Gly Ser Gly
325 330
<210> 284
<211> 358
<212> PRT
<213> 拟南芥()
<220>
<223> 转录因子NAC次生壁增厚促进因子3 (NST3, AtNST3, SND1, ANAC012)
<400> 284
Met Ala Asp Asn Lys Val Asn Leu Ser Ile Asn Gly Gln Ser Lys Val
1 5 10 15
Pro Pro Gly Phe Arg Phe His Pro Thr Glu Glu Glu Leu Leu His Tyr
20 25 30
Tyr Leu Arg Lys Lys Val Asn Ser Gln Lys Ile Asp Leu Asp Val Ile
35 40 45
Arg Glu Val Asp Leu Asn Lys Leu Glu Pro Trp Asp Ile Gln Glu Glu
50 55 60
Cys Arg Ile Gly Ser Thr Pro Gln Asn Asp Trp Tyr Phe Phe Ser His
65 70 75 80
Lys Asp Lys Lys Tyr Pro Thr Gly Thr Arg Thr Asn Arg Ala Thr Val
85 90 95
Ala Gly Phe Trp Lys Ala Thr Gly Arg Asp Lys Ile Ile Cys Ser Cys
100 105 110
Val Arg Arg Ile Gly Leu Arg Lys Thr Leu Val Phe Tyr Lys Gly Arg
115 120 125
Ala Pro His Gly Gln Lys Ser Asp Trp Ile Met His Glu Tyr Arg Leu
130 135 140
Asp Asp Thr Pro Met Ser Asn Gly Tyr Ala Asp Val Val Thr Glu Asp
145 150 155 160
Pro Met Ser Tyr Asn Glu Glu Gly Trp Val Val Cys Arg Val Phe Arg
165 170 175
Lys Lys Asn Tyr Gln Lys Ile Asp Asp Cys Pro Lys Ile Thr Leu Ser
180 185 190
Ser Leu Pro Asp Asp Thr Glu Glu Glu Lys Gly Pro Thr Phe His Asn
195 200 205
Thr Gln Asn Val Thr Gly Leu Asp His Val Leu Leu Tyr Met Asp Arg
210 215 220
Thr Gly Ser Asn Ile Cys Met Pro Glu Ser Gln Thr Thr Thr Gln His
225 230 235 240
Gln Asp Asp Val Leu Phe Met Gln Leu Pro Ser Leu Glu Thr Pro Lys
245 250 255
Ser Glu Ser Pro Val Asp Gln Ser Phe Leu Thr Pro Ser Lys Leu Asp
260 265 270
Phe Ser Pro Val Gln Glu Lys Ile Thr Glu Arg Pro Val Cys Ser Asn
275 280 285
Trp Ala Ser Leu Asp Arg Leu Val Ala Trp Gln Leu Asn Asn Gly His
290 295 300
His Asn Pro Cys His Arg Lys Ser Phe Asp Glu Glu Glu Glu Asn Gly
305 310 315 320
Asp Thr Met Met Gln Arg Trp Asp Leu His Trp Asn Asn Asp Asp Asn
325 330 335
Val Asp Leu Trp Ser Ser Phe Thr Glu Ser Ser Ser Ser Leu Asp Pro
340 345 350
Leu Leu His Leu Ser Val
355
<210> 285
<211> 337
<212> PRT
<213> 火炬松()
<220>
<223> 松树转录因子NAC次生壁增厚促进因子 (PtNAC023)
<400> 285
Met Glu Ala Lys Ala Ser Ser Gly Ile Gln Leu Pro Pro Gly Phe Arg
1 5 10 15
Phe His Pro Ser Asp Glu Glu Leu Ile Val His Tyr Leu Lys Asn Arg
20 25 30
Val Ser Ser Ser Pro Leu Pro Ala Ser Ile Ile Ala Glu Ile Asp Leu
35 40 45
Tyr Lys Tyr Asn Pro Trp Glu Leu Pro Lys Lys Ala Leu Phe Gly Val
50 55 60
Asp Glu Trp Tyr Phe Phe Thr Pro Arg Asp Arg Lys Tyr Pro Asn Gly
65 70 75 80
Ala Arg Pro Asn Arg Ala Ala Ala Ser Gly Tyr Trp Lys Ala Thr Gly
85 90 95
Thr Asp Lys Ser Ile Phe Asn Ser Phe Gly Thr Ala Lys Ile Gly Val
100 105 110
Lys Lys Thr Leu Val Phe Tyr Glu Gly Arg Pro Pro Lys Gly Leu Lys
115 120 125
Thr Asp Trp Asn Met His Glu Tyr Arg Leu Leu Asp Ala Met Ile Trp
130 135 140
Asn Asn Ser Lys Arg Lys Gly Ser Met Arg Leu Asp Asp Trp Val Leu
145 150 155 160
Cys Arg Val Arg Gln Lys Asn Ser Ile Pro Arg Asn Thr Trp Gln Asp
165 170 175
Gln Asn Ile Pro Ser Cys Ala Ser Ala Pro Thr Gly Leu Phe Pro Lys
180 185 190
Val Asn Glu Leu Gln Gln Met Asn Ile Asn Pro Asn Thr Glu Met Val
195 200 205
Thr Asn Tyr Phe Tyr Asp Asp Cys Pro Met Leu Pro Cys Ile Phe Ser
210 215 220
Pro Gln Asn Phe Pro Ser Thr Glu Arg Ala Ser Ser Ile Asn Phe Leu
225 230 235 240
Ser Ser Asp Lys Ser Cys Thr Ser Phe His Asn Leu Leu Asn Leu Leu
245 250 255
Lys Arg Lys Pro Ala Glu Thr Asn Gln Gln Arg Gly Asn Cys Phe Pro
260 265 270
Pro Gly Lys Met Leu Arg Thr Lys Ala Asp Ile Glu Glu Asp Val Val
275 280 285
Ser Ile Arg Asn Asp Gly Thr Asp Glu Asn Phe Ser Gly Thr Asp Gln
290 295 300
Ser Glu Ser Gly Asn Phe Ser Thr Glu Gln Trp Asn Ser Pro Met Gln
305 310 315 320
Tyr Gln Glu Cys Asn His Leu Thr Phe Thr Gly Asn His Leu Thr Leu
325 330 335
Asp
<210> 286
<211> 319
<212> PRT
<213> 蒺藜状苜蓿()
<220>
<223> 转录因子NAC次生壁增厚促进因子1 (MtNAC1)
<400> 286
Met Pro Asp Asn Met Ser Ile Ser Val Asn Gly Gln Ser Gln Val Pro
1 5 10 15
Pro Gly Phe Arg Phe His Pro Thr Glu Glu Glu Leu Leu Gln Tyr Tyr
20 25 30
Leu Arg Lys Lys Val Ser Tyr Glu Lys Ile Asp Leu Asp Val Ile Arg
35 40 45
Asp Val Asp Leu Asn Lys Leu Glu Pro Trp Asp Ile Gln Glu Lys Cys
50 55 60
Lys Ile Gly Thr Thr Pro Gln Asn Asp Trp Tyr Phe Phe Ser His Lys
65 70 75 80
Asp Lys Lys Tyr Pro Thr Gly Thr Arg Thr Asn Arg Ala Thr Ala Ala
85 90 95
Gly Phe Trp Lys Ala Thr Gly Arg Asp Lys Val Ile Tyr Ser Asn Gly
100 105 110
Lys Arg Ile Gly Met Arg Lys Thr Leu Val Phe Tyr Lys Gly Arg Ala
115 120 125
Pro His Gly Gln Lys Ser Asp Trp Ile Met His Glu Tyr Arg Leu Asp
130 135 140
Asp Asn Thr Thr Asn Asp Thr Asn Leu Val Ser Asn Met Ile Gly Asp
145 150 155 160
Gly Gly Gln Glu Glu Gly Trp Val Val Cys Arg Ile Phe Lys Lys Lys
165 170 175
Asn His Leu Lys Thr Leu Asp Ser Pro Ser Gly Glu Gly Arg Arg Ser
180 185 190
His His Leu Tyr Asp Thr Cys Asp Glu Gly Ala Leu Glu Gln Ile Leu
195 200 205
Gln Gln Met Gly Arg Gly Cys Lys Glu Glu Asn Tyr Glu Ala Asn Tyr
210 215 220
Asn Asn Asn Tyr Gly Arg Phe Ala Arg Pro Phe Glu Ser Thr Leu Asn
225 230 235 240
Asn Asn Gly Gly Tyr Asn Asn Glu Arg Phe Met Lys Leu Pro Asn Leu
245 250 255
Glu Ser Pro Lys Ser Thr Ser Met Glu Asn Asn Glu Asn Asn Asn Asp
260 265 270
Gly Tyr His Ala Ile Ile Gln Val Asp Met Ala Asn Glu Asn Glu Gly
275 280 285
Ser Phe Ser Asp His His His His His His His Asn Asn Met Val Asn
290 295 300
Asn Pro Leu Glu Ala Ser Ser Ser Ser Met Val Ile Ser Met Trp
305 310 315
<210> 287
<211> 221
<212> PRT
<213> 大豆()
<220>
<223> 大豆转录因子NAC次生壁增厚促进因子 (GmNAM1)
<400> 287
Met Pro Glu Asn Met Ser Ile Ser Val Asn Gly Gln Ser Gln Val Pro
1 5 10 15
Pro Gly Phe Arg Phe His Pro Thr Glu Glu Glu Leu Leu Gln Tyr Tyr
20 25 30
Leu Arg Lys Lys Val Ser Tyr Glu Lys Ile Asp Leu Asp Val Ile Arg
35 40 45
Asp Val Asp Leu Asn Lys Leu Glu Pro Trp Asp Ile Gln Glu Lys Cys
50 55 60
Lys Ile Gly Thr Thr Pro Gln Asn Asp Trp Tyr Phe Phe Ser His Lys
65 70 75 80
Asp Lys Lys Tyr Pro Thr Gly Thr Arg Thr Asn Arg Ala Thr Ala Ala
85 90 95
Gly Phe Trp Lys Ala Thr Gly Arg Asp Lys Val Ile Tyr Ser Asn Gly
100 105 110
Lys Arg Ile Gly Met Arg Lys Thr Leu Val Phe Tyr Lys Gly Arg Ala
115 120 125
Pro His Gly Gln Lys Ser Asp Trp Ile Met His Glu Tyr Arg Leu Asp
130 135 140
Asp Asn Asn Thr Ala Asp Thr Asn Ile Val Ser Asn Val Met Gly Asp
145 150 155 160
Ala Ala Gln Glu Glu Gly Trp Val Val Cys Arg Ile Phe Lys Lys Lys
165 170 175
Asn His Leu Lys Thr Leu Asp Ser Pro Leu Ala Ser Gly Glu Asp Arg
180 185 190
Arg Ser Pro Lys Ser Ala Ser Met Glu Ser His His Asn Thr Asn Asn
195 200 205
Asn Asn Asn Met Asn Ser Asn Asn Ile Lys Lys Lys Asn
210 215 220
<210> 288
<211> 379
<212> PRT
<213> 火炬松()
<220>
<223> 松树转录因子NAC次生壁增厚促进因子 (PtNAC065)
<400> 288
Met Thr Glu Asn Met Ser Ile Ser Val Asn Gly Gln Ser Gln Val Pro
1 5 10 15
Pro Gly Phe Arg Phe His Pro Thr Glu Glu Glu Leu Leu His Tyr Tyr
20 25 30
Leu Arg Lys Lys Val Ser Tyr Glu Lys Ile Asp Leu Asp Val Ile Arg
35 40 45
Asp Val Asp Leu Asn Lys Leu Glu Pro Trp Asp Ile Gln Glu Arg Cys
50 55 60
Lys Ile Gly Thr Thr Pro Gln Asn Asp Trp Tyr Phe Phe Ser His Lys
65 70 75 80
Asp Lys Lys Tyr Pro Thr Gly Thr Arg Thr Asn Arg Ala Thr Ala Ala
85 90 95
Gly Phe Trp Lys Ala Thr Gly Arg Asp Lys Val Ile Tyr Ser Thr Gly
100 105 110
Lys Arg Ile Gly Met Arg Lys Thr Leu Val Phe Tyr Lys Gly Arg Ala
115 120 125
Pro His Gly Gln Lys Ser Asp Trp Ile Met His Glu Tyr Arg Leu Asp
130 135 140
Asp Ser Thr Ser Asp Thr Asn Val Ser Asn Val Met Glu Glu Glu Ala
145 150 155 160
Ala Gln Glu Glu Gly Trp Val Val Cys Arg Ile Phe Lys Lys Lys Asn
165 170 175
Leu Asn Lys Thr Leu Asp Lys Pro Phe Ser Ser Ser Pro Ile Ser Ala
180 185 190
Asp Thr Arg Asn Gln Met Leu Ser Ser Cys Asp Glu Gly Thr Ile Asp
195 200 205
Gln Thr Phe His Tyr Met Gly Arg Thr Cys Lys Glu Glu Asn Val Ala
210 215 220
Asp Asn Ser Ala Thr Ala Arg Tyr Leu Arg Pro Val Asp Thr Ala Ile
225 230 235 240
Asn Tyr Val His His Asp Gly Phe Met Lys Leu Pro Ser Leu Glu Ser
245 250 255
Pro Asn Ser Ile Ser Ser Gln Asn Cys Tyr Gln Pro Met Ile Thr Asp
260 265 270
Asn Glu Gly Ser Ile Thr Asn Gln Met Ser Tyr Pro Leu Asp Pro Gly
275 280 285
Leu Asp Asn Trp Ala Thr Leu Asp Arg Leu Phe Ala Tyr Gln Leu Asn
290 295 300
Gly Gln Thr Glu Thr Ser Arg Gln Leu Pro Cys Ile Asp Pro Thr Ile
305 310 315 320
Thr Tyr Cys Thr Pro Ser Thr Asp Leu His His Asp Leu Arg Leu Pro
325 330 335
Thr Leu Arg Ser Ser Phe Pro Leu Pro Ser Asn Arg Ser Tyr His Gly
340 345 350
Thr Gln Asp Tyr Asn Asn Glu Ile Asp Leu Trp Asn Phe Thr Thr Arg
355 360 365
Ser Ser Pro Asp Thr Leu Cys Gln Leu Ser Asn
370 375
<210> 289
<211> 390
<212> PRT
<213> 葡萄()
<220>
<223> 葡萄转录因子NAC次生壁增厚促进因子 (VvNST)
<400> 289
Met Pro Glu Ser Met Ser Ile Ser Val Asn Gly Gln Ser Gln Val Pro
1 5 10 15
Pro Gly Phe Arg Phe His Pro Thr Glu Glu Glu Leu Leu Asn Tyr Tyr
20 25 30
Leu Arg Lys Lys Val Leu Tyr Glu Lys Ile Asp Leu Asp Val Ile Arg
35 40 45
Asp Val Asp Leu Asn Lys Leu Glu Pro Trp Asp Ile Gln Glu Lys Cys
50 55 60
Arg Ile Gly Ser Thr Pro Gln Asn Asp Trp Tyr Phe Phe Ser His Lys
65 70 75 80
Asp Lys Lys Tyr Pro Thr Gly Thr Arg Thr Asn Arg Ala Thr Ala Ala
85 90 95
Gly Phe Trp Lys Ala Thr Gly Arg Asp Lys Val Ile Tyr Ser Ser Phe
100 105 110
Lys Arg Ile Gly Met Arg Lys Thr Leu Val Phe Tyr Lys Gly Arg Ala
115 120 125
Pro His Gly Gln Lys Ser Asp Trp Ile Met His Glu Tyr Arg Leu Asp
130 135 140
Asp Asn Thr Asn Asp Thr Gln Val Ser Asn Ala Val Ala Glu Ala Gln
145 150 155 160
Glu Glu Gly Trp Val Val Cys Arg Ile Phe Lys Lys Lys Asn His His
165 170 175
Lys Thr Val Glu Ser Ser Ser Pro Met Ser Ser Ser Ser Ile Thr Ala
180 185 190
Glu Thr Arg Thr Gln Leu Leu Asn Ser Ser Asp Glu Gly Ala Leu Glu
195 200 205
Gln Ile Phe Gln His Met Gly Arg Thr Cys Lys Glu Asp Asn Asn Glu
210 215 220
Ala Asn Asn Ser Arg Arg Phe Leu Arg Ser Ile Asp Thr Val Ile Asn
225 230 235 240
Asn Gly Ser Thr Leu His Asp Arg Phe Met Lys Leu Pro Thr Leu Glu
245 250 255
Ser Pro Asn Ser Thr Ser Ser Gln Asn Cys Tyr Gln Pro Ile His Val
260 265 270
Gln Met Ile Pro Glu Asn Glu Ala Ala Pro Ile Thr Pro His Pro Val
275 280 285
Tyr His Leu Asp Thr Gly Leu Asn Asp Asp Trp Ala Ala Leu Asp Arg
290 295 300
Leu Val Ala Ser His Leu Asn Gly Gln Thr Asp Thr Ser Lys Gln Leu
305 310 315 320
Ala Cys Phe Asn Asp Pro Ser Met Pro Phe Ser Ser Pro Thr Asp His
325 330 335
Asn Asp His Asp Ser Gln Leu Leu Gln Pro Arg Ser Ser Ser Ser Thr
340 345 350
Thr Arg Ser Tyr His Pro Thr Gln Asp Tyr Asn Thr Glu Ile Asp Leu
355 360 365
Trp Ser Phe Thr Arg Ser Cys Leu Gln Ser Ser Asp Pro Leu Cys His
370 375 380
Ile Ser Asn Thr Pro Ser
385 390
<210> 290
<211> 348
<212> PRT
<213> 蓖麻()
<220>
<223> 转录因子NAC次生壁增厚促进因子 (RcNST)
<400> 290
Met Pro Glu Asn Met Ser Ile Ser Val Asn Gly Gln Ser Gln Val Pro
1 5 10 15
Pro Gly Phe Arg Phe His Pro Thr Glu Glu Glu Leu Leu Tyr Tyr Tyr
20 25 30
Leu Lys Lys Lys Val Ser Tyr Glu Lys Ile Asp Leu Asp Val Ile Arg
35 40 45
Asp Val Asp Leu Asn Lys Leu Glu Pro Trp Asp Ile Gln Glu Lys Cys
50 55 60
Lys Ile Gly Thr Thr Pro Gln Asn Asp Trp Tyr Phe Phe Ser His Lys
65 70 75 80
Asp Lys Lys Tyr Pro Thr Gly Thr Arg Thr Asn Arg Ala Thr Ala Ala
85 90 95
Gly Phe Trp Lys Ala Thr Gly Arg Asp Lys Val Ile Tyr Ser Asn Gly
100 105 110
Arg Arg Ile Gly Met Arg Lys Thr Leu Val Phe Tyr Lys Gly Arg Ala
115 120 125
Pro His Gly Gln Lys Ser Asp Trp Ile Met His Glu Tyr Arg Leu Asp
130 135 140
Asp Asn Ile Val Asp Thr Asn Val Ser Asn Val Met Gly Glu Ala Ala
145 150 155 160
Gln Glu Glu Gly Trp Val Val Cys Arg Ile Phe Lys Lys Lys Asn His
165 170 175
His Lys Thr Leu Asp Ser Pro Leu Ser Ser Ser Ser Phe Phe Thr Glu
180 185 190
Ala Arg Thr Gln Ile Phe Asn Ser Cys Asn Glu Gly Ala Leu Gly Gln
195 200 205
Ile Leu Glu Glu Tyr Met Gly Arg Thr Cys Lys Glu Glu Asn Glu Ala
210 215 220
Asn Asn Cys Val Arg Phe Leu Arg Pro Ile Asp Thr Thr Ile Asn Ser
225 230 235 240
Gly Ser Ser Tyr His Asp Lys Phe Met Lys Leu Pro Ser Leu Glu Ser
245 250 255
Pro Asn Ser Ser Ser Ser Gln Asn Ala Tyr Pro Pro Met Ile Asn Asn
260 265 270
Asn Glu Ser Ser Val Ser Asn Gln Leu Asn Ser Met Asp Ala Asn Asn
275 280 285
Ser Ser Leu Tyr Pro Leu Glu Ser Gly Ile Thr Asn Trp Ala Ala Leu
290 295 300
Asp Arg Leu Val Ala Ser Gln Leu Asn Gly Gln Thr Glu Ala Ser Arg
305 310 315 320
Gln Leu Ala Cys Phe Ser Asp Pro Thr Met Val Tyr Cys Asn Pro Ile
325 330 335
Asn Gln Pro His His Asp Leu Gln Leu Leu Lys Ser
340 345
<210> 291
<211> 383
<212> PRT
<213> 冈尼桉()
<220>
<223> 转录因子NAC次生壁增厚促进因子 (EgNST)
<400> 291
Met Asp Met Asn Leu Ser Ile Asn Gly Gln Ser Gln Ile Pro Pro Gly
1 5 10 15
Phe Arg Phe His Pro Thr Glu Glu Glu Leu Leu His Tyr Tyr Leu Arg
20 25 30
Lys Lys Met Ser Asn Lys Lys Ile Asp Leu Asp Val Ile Arg Asp Val
35 40 45
Asp Leu Asn Lys Leu Glu Pro Trp Asp Ile Gln Glu Arg Cys Lys Ile
50 55 60
Gly Ser Gly Pro Gln Ser Asp Trp Tyr Phe Phe Ser His Lys Asp Lys
65 70 75 80
Lys Tyr Pro Thr Gly Thr Arg Thr Asn Arg Ala Thr Ala Val Gly Phe
85 90 95
Trp Lys Ala Thr Gly Arg Asp Lys Ile Ile Tyr Gly Gly Ser Ser Arg
100 105 110
Ile Gly Leu Arg Lys Thr Leu Val Phe Tyr Lys Gly Arg Ala Pro His
115 120 125
Gly Gln Lys Ser Asp Trp Ile Met His Glu Tyr Arg Leu Asp Glu Gly
130 135 140
Ser Asn Ser Ser Val Gly Pro Ser Leu Val Cys Glu Ser Ser Pro Glu
145 150 155 160
Asp Gly Trp Val Val Cys Arg Val Phe Arg Lys Lys Ser His Gln Arg
165 170 175
Thr Leu Glu Ser Pro Lys Ser Thr Ser Thr Ser Ser Met Asp Thr Glu
180 185 190
Met Leu Met Asn Ala Ser Ala Asp Ser Ser Val Leu Asp Gln Ile Leu
195 200 205
Ser Tyr Met Gly Arg Thr Asn Cys Glu Ser Pro Glu Asn Tyr Gln Ala
210 215 220
Pro Ile Asn Ile Ser Ser Met Gln Thr Gln Leu Ile His Gly Lys Phe
225 230 235 240
Leu His Leu Pro Arg Leu Asp Asp Ser Ser His Leu Gly Gln Glu Thr
245 250 255
Gly Leu Arg Thr Phe Tyr Gln Lys Phe Asp Glu Ile Arg Ile Pro Gly
260 265 270
Ser Asp Asp Pro Ser Ser Lys His Thr Ser Ser Asp His Glu Pro Lys
275 280 285
Ala Gly Thr Ala Gly Ile Asn Asp Trp Val Ala Leu Asp Arg Leu Val
290 295 300
Ala Ser Gln Leu Asn Gly Gln Leu Glu Ile Ser Ala Ala Ser Lys Gln
305 310 315 320
Ser Pro Tyr Pro Gly Ala Pro Asn Pro Val Leu Ala Gly Pro Thr Pro
325 330 335
Gly Asp His Gln Val Gly Gln Leu Ser Leu Leu Gln Leu Asn Arg Pro
340 345 350
Asn Gln Ile Tyr Ser Ser Glu Ser Ser Phe Asp Trp Ser Phe Gly Lys
355 360 365
Ser Ser Ser Val Thr Pro Ser Asn Pro Leu His His Leu Ser Val
370 375 380
<210> 292
<211> 380
<212> PRT
<213> 玉米()
<220>
<223> 玉米转录因子NAC次生壁增厚促进因子 (ZmNST)
<400> 292
Met Ser Ile Ser Val Asn Gly Gln Ser Cys Val Pro Pro Gly Phe Arg
1 5 10 15
Phe His Pro Thr Glu Glu Glu Leu Leu Asn Tyr Tyr Leu Arg Lys Lys
20 25 30
Val Ala Ser Gln Gln Ile Asp Leu Asp Val Ile Arg Asp Val Asp Leu
35 40 45
Asn Lys Leu Glu Pro Trp Asp Ile Gln Glu Arg Cys Lys Ile Gly Ser
50 55 60
Gly Pro Gln Asn Asp Trp Tyr Phe Phe Ser His Lys Asp Lys Lys Tyr
65 70 75 80
Pro Thr Gly Thr Arg Thr Asn Arg Ala Thr Ala Ala Gly Phe Trp Lys
85 90 95
Ala Thr Gly Arg Asp Lys Ala Ile Tyr Ser Ala Val Arg Arg Met Gly
100 105 110
Met Arg Lys Thr Leu Val Phe Tyr Arg Gly Arg Ala Pro His Gly His
115 120 125
Lys Ser Asp Trp Ile Met His Glu Tyr Arg Leu Asp Asp Pro Asp Ala
130 135 140
Ala Ala Val Ala Ala Thr Val Ala Ala Ala Ala Ala Ser Ser Asp Gly
145 150 155 160
Gly Gln Glu Asp Gly Trp Val Val Cys Arg Val Phe Gln Lys Lys His
165 170 175
His His Lys Glu Ser Ser Gly Arg Cys Arg Ser Lys Arg Gly Ser Lys
180 185 190
Thr Glu His Gly His Gly Glu Ala Lys Thr Ala Ala His Gln Arg His
195 200 205
Gly Cys Gly Leu Gln Tyr Ser Ser Asn Asp Asp Thr Leu Asp His Met
210 215 220
Leu Gly Arg Arg Ser Cys Lys Gln Glu His Glu Leu Leu Pro Leu Pro
225 230 235 240
Pro Pro Ala Ala Ala Arg Ala Ala Ser Arg Tyr Ile Arg Pro Ile Glu
245 250 255
Thr Val Leu Gly Gly His Gly Phe Met Lys Leu Pro Pro Leu Glu Ser
260 265 270
Pro Ala Ala Ala Glu Ala Leu Thr Thr Pro His Ala Val Ser Ala Gly
275 280 285
Asp Ala Thr Ala Ala Gly Ala Leu Asp Gly Leu His Arg Ala Gly Asn
290 295 300
Gly Ile Thr Asp Trp Val Met Met Asp Arg Met Val Ala Leu His Leu
305 310 315 320
Asn Gly Gln Ala Pro Ala Ala Asp Gln Leu Gly Ser Cys Phe Asp Ala
325 330 335
Ser Ala Asp Gly Gly Gly Leu Ala Cys Phe Tyr Ser Ala Ala Ala Asn
340 345 350
Arg Leu Leu Gly Gly Gly Asp Asp Asp Leu Trp Ser Phe Thr Arg Ser
355 360 365
Ser Ser Thr Glu Arg Leu Gly His Val Ser Leu Met
370 375 380
<210> 293
<211> 424
<212> PRT
<213> 两色高梁()
<220>
<223> 高粱转录因子NAC次生壁增厚促进因子 (SbNST)
<400> 293
Met Ser Ile Ser Val Asn Gly Gln Ser Cys Val Pro Pro Gly Phe Arg
1 5 10 15
Phe His Pro Thr Glu Glu Glu Leu Leu Asn Tyr Tyr Leu Arg Lys Lys
20 25 30
Val Ala Ser Gln Glu Ile Asp Leu Asp Val Ile Arg Asp Val Asp Leu
35 40 45
Asn Lys Leu Glu Pro Trp Asp Ile Gln Glu Lys Cys Lys Ile Gly Ser
50 55 60
Gly Pro Gln Asn Asp Trp Tyr Phe Phe Ser His Lys Asp Lys Lys Tyr
65 70 75 80
Pro Thr Gly Thr Arg Thr Asn Arg Ala Thr Ala Ala Gly Phe Trp Lys
85 90 95
Ala Thr Gly Arg Asp Lys Ala Ile Tyr Asn Ala Val Lys Arg Ile Gly
100 105 110
Met Arg Lys Thr Leu Val Phe Tyr Lys Gly Arg Ala Pro His Gly Gln
115 120 125
Lys Ser Asp Trp Ile Met His Glu Tyr Arg Leu Asp Asp Pro Ala Ala
130 135 140
Ser Gly Asp Ala Ala Ala Ala Ala Thr Ala Ala Ala Ala Ala Thr Val
145 150 155 160
Ala Ala Ala Ala Ala Ser Ser Asp Gly Gly Gln Glu Asp Ala Trp Val
165 170 175
Val Tyr Arg Val Phe Lys Lys Lys His His His Lys Glu Ser Ser Gly
180 185 190
Gly Gly Gly Gly Ser Lys His Gly Gly Ser Asn Asn Glu His Gly His
195 200 205
Gly Gly Gly Lys Ala Ala Ala Ala Ala Ala Ala Ala Ala His Gln His
210 215 220
His Gly Gly Leu Gln Tyr Ser Ser Ser Asp Asp Ala Leu Asp Gln Ile
225 230 235 240
Leu Gln Tyr Met Gly Arg Ser Cys Lys Gln Glu His Glu Leu Leu Ser
245 250 255
Pro Pro Pro Pro Gly Arg Ala Ala Ser Arg Tyr Leu Arg Pro Ile Glu
260 265 270
Thr Val Leu Gly Gly His Gly Phe Met Lys Leu Pro Pro Leu Glu Ser
275 280 285
Pro Ser Ala Ala Ala Ala Met Thr Pro Gln Ala Val Ser Gly Asp Ala
290 295 300
Gly Val Val Asp Asp Leu Leu Gly Leu His Arg Gly Gly Ile Gly Asn
305 310 315 320
Gly Ile Thr Asp Trp Ala Met Met Asp Arg Leu Val Ala Ser His Leu
325 330 335
Asn Gly Gln Glu Ala Pro Asp Val Ala Pro Ala Ala Asp Gln Leu Gly
340 345 350
Ser Cys Phe Asp Asp Ala Thr Gly Ala Asp Asp Ala Asp Ala Ala Gly
355 360 365
Leu Ala Phe Tyr Ser Ala Ala Ala Asn Arg Leu Leu Val Gly Ser Ala
370 375 380
Gly Ser Ser Gly Ala Gly Ser Asp Asp Asp Leu Trp Ser Phe Thr Arg
385 390 395 400
Ser Ser Ala Ala Ala Ala Ala Ala Thr Ser Thr Glu Arg Leu Ser His
405 410 415
Val Ser Leu Met Ser Ile Ser Val
420
<210> 294
<211> 395
<212> PRT
<213> 水稻()
<220>
<223> 稻转录因子NAC次生壁增厚促进因子 (OsNAC7)
<400> 294
Met Ser Ile Ser Val Asn Gly Gln Ser Val Val Pro Pro Gly Phe Arg
1 5 10 15
Phe His Pro Thr Glu Glu Glu Leu Leu Thr Tyr Tyr Leu Lys Lys Lys
20 25 30
Val Ala Ser Glu Arg Ile Asp Leu Asp Val Ile Arg Asp Val Asp Leu
35 40 45
Asn Lys Leu Glu Pro Trp Asp Ile Gln Glu Arg Cys Arg Ile Gly Ser
50 55 60
Gly Pro Gln Asn Asp Trp Tyr Phe Phe Ser His Lys Asp Lys Lys Tyr
65 70 75 80
Pro Thr Gly Thr Arg Thr Asn Arg Ala Thr Ala Ala Gly Phe Trp Lys
85 90 95
Ala Thr Gly Arg Asp Lys Ala Ile Tyr Ser Ser Ser Asn Arg Ile Gly
100 105 110
Met Arg Lys Thr Leu Val Phe Tyr Lys Gly Arg Ala Pro His Gly Gln
115 120 125
Lys Ser Asp Trp Ile Met His Glu Tyr Arg Leu Asp Asp Pro Ser Ser
130 135 140
Ala Ser Ala Ser Val Ser Val Asn Leu Pro Ser Tyr Tyr Ser Ser Ser
145 150 155 160
Ser Ser Ser Ser Ser Pro Met His Gly Val Ala Gly Asp Gln Gly Ala
165 170 175
Gln Glu Glu Gly Trp Val Ile Cys Arg Val Phe Lys Lys Lys Asn Leu
180 185 190
Val His His Gly Gly Gly Ala Ala Ala Ala Ser His His Ala Ala Ala
195 200 205
Lys Leu Ala Ala Ala Ala Met Glu Gly Ser Pro Ser Asn Cys Ser Thr
210 215 220
Val Thr Val Ser Asp His Val Lys Ala Gln Met Leu His Ser Ser Ala
225 230 235 240
Ser Asp Asp Ala Leu Asp His Ile Leu Gln Tyr Met Gly Arg Ser Gly
245 250 255
Cys Lys Gln Glu Thr Lys Pro Ala Ala Met Ser Ala Ser Ser Ala Ala
260 265 270
Ala Ala Ala Ala Leu Glu Gln His Leu Ser Thr Pro Gln Tyr Gly Lys
275 280 285
Phe Met Lys Leu Pro Pro Leu Glu His Val Ala Gly Gly Val Gly Leu
290 295 300
Leu Ala Ala Ala Gly Gly Gly Gly Glu Tyr Cys Ser Ala Ala Asp Ala
305 310 315 320
Ser Gly Ile Ala Asp Trp Asp Thr Leu Asp Arg Leu Ala Ala Ser Tyr
325 330 335
Glu Leu Asn Gly Ala Leu Ser Asp Val Ala Ser Gly Lys Asn Met Ala
340 345 350
Gly Phe Phe Asp Val Val Asp Gln Pro Ala Gly Ala Ala Ala Phe Ser
355 360 365
Ser Gly Asp Gly Asp Leu Trp Ser Leu Ala Arg Ser Val Ser Ser Ser
370 375 380
Leu His Ala Asp Leu Thr Thr Met Asn Asn Val
385 390 395
<210> 295
<211> 428
<212> PRT
<213> 两色高梁()
<220>
<223> 高粱转录因子NAC次生壁增厚促进因子 (SbNST)
<400> 295
Met Ser Ile Ser Val Asn Gly Gln Ser Val Val Pro Pro Gly Phe Arg
1 5 10 15
Phe His Pro Thr Glu Glu Glu Leu Leu Thr Tyr Tyr Leu Lys Lys Lys
20 25 30
Val Ala Ser Glu Arg Ile Asp Leu Asp Val Ile Arg Asp Val Asp Leu
35 40 45
Asn Lys Leu Glu Pro Trp Asp Ile Gln Glu Lys Cys Arg Ile Gly Ser
50 55 60
Gly Pro Gln Asn Asp Trp Tyr Phe Phe Ser His Lys Asp Lys Lys Tyr
65 70 75 80
Pro Thr Gly Thr Arg Thr Asn Arg Ala Thr Ala Ala Gly Phe Trp Lys
85 90 95
Ala Thr Gly Arg Asp Lys Ala Ile Tyr Ala Ser Gly Ala Arg Arg Ile
100 105 110
Gly Met Arg Lys Thr Leu Val Phe Tyr Lys Gly Arg Ala Pro His Gly
115 120 125
Gln Lys Ser Asp Trp Ile Met His Glu Tyr Arg Leu Glu Pro Ala Leu
130 135 140
Asp Val Asp Ala Ala Ala Gly Ser Ala Ser Ala His His Ala Ala Ala
145 150 155 160
Gly Ala Ala Ala Asp His His Pro Tyr Tyr Thr Ser Ser Ser Pro Pro
165 170 175
Ala Leu Pro Thr Ala Ile Arg Gly Ala Ala Gly Asp Gln Gln Ala Ala
180 185 190
Gln Glu Gln Glu Gly Trp Val Ile Cys Arg Val Phe Lys Lys Lys Asn
195 200 205
Leu Val His His Gly Gln Ser Ser Gly Gly Gly Val Thr Ala Ala Gly
210 215 220
Ser Lys Met Ala Ser Ala Ala Ala Pro Met Glu Gly Ser Pro Ser His
225 230 235 240
Cys Ser Ser Val Thr Val Ile Ser Asp His Thr Met Asn Lys His Gln
245 250 255
Ala Gln Ala Met Leu Gln His Ser Ala Ser Asp Asp Asp Ala Leu Asp
260 265 270
His Ile Leu Gln Tyr Met Gly Gly Gly Gly Gly Lys Gln Pro Asp Thr
275 280 285
Lys Pro Val Leu Leu Asp His His His His His His Leu Ala Ala Ala
290 295 300
Ala Thr Thr Thr Thr Thr Ala Cys Ser Ala Gly Gly Ala Gly Leu Tyr
305 310 315 320
Gly Lys Phe Met Lys Leu Pro Pro Leu Glu His Ala Gly Gly Gly Gly
325 330 335
Gly Leu Leu Pro Ser Pro Ala Gly Ala Cys Asp Tyr Gly Ala Ala Asp
340 345 350
Ala Ser Gly Ile Ala Asp Trp Asp Ala Leu Asp Arg Leu Ala Ala Tyr
355 360 365
Glu Leu Asn Gly Leu Ser Asp Ala Ser Lys Asn Met Ser Ala Phe Phe
370 375 380
Asp Glu Pro Ser Ala Thr Ala Ala Phe Ser Ser Ser Ser Ser Ser Val
385 390 395 400
His Ala Ala Ala Val Asp Gly Asp Leu Trp Ser Leu Ala Arg Ser Val
405 410 415
Ser Ala Leu His Ala Asp Leu Thr Met Asn Asn Val
420 425
<210> 296
<211> 401
<212> PRT
<213> 火炬松()
<220>
<223> 松树转录因子NAC次生壁增厚促进因子 (PtNAC)
<400> 296
Met Ser Ile Ser Val Asn Gly Gln Ser Gln Val Pro Pro Gly Phe Arg
1 5 10 15
Phe His Pro Thr Glu Glu Glu Leu Leu His Tyr Tyr Leu Arg Lys Lys
20 25 30
Val Ser Tyr Glu Lys Ile Asp Leu Asp Val Ile Arg Asp Val Asp Leu
35 40 45
Asn Lys Leu Glu Pro Trp Asp Ile Gln Glu Arg Cys Lys Ile Gly Thr
50 55 60
Thr Pro Gln Asn Asp Trp Tyr Phe Phe Ser His Lys Asp Lys Lys Tyr
65 70 75 80
Pro Thr Gly Thr Arg Thr Asn Arg Ala Thr Ala Ala Gly Phe Trp Lys
85 90 95
Ala Thr Gly Arg Asp Lys Val Ile Tyr Ser Thr Gly Lys Arg Ile Gly
100 105 110
Met Arg Lys Thr Leu Val Phe Tyr Lys Gly Arg Ala Pro His Gly Gln
115 120 125
Lys Ser Asp Trp Ile Met His Glu Tyr Arg Leu Asp Asp Ser Thr Ser
130 135 140
Asp Thr Asn Val Ser Asn Val Met Glu Glu Glu Ala Ala Gln Glu Glu
145 150 155 160
Gly Trp Val Val Cys Arg Ile Phe Lys Lys Lys Asn Leu Asn Lys Thr
165 170 175
Leu Asp Lys Pro Phe Ser Ser Ser Pro Ile Ser Ala Asp Thr Arg Asn
180 185 190
Gln Met Leu Ser Ser Cys Asp Glu Gly Thr Ile Asp Gln Thr Phe His
195 200 205
Tyr Met Gly Arg Thr Cys Lys Glu Glu Asn Val Ala Asp Asn Ser Ala
210 215 220
Thr Ala Arg Tyr Leu Arg Pro Val Asp Thr Ala Ile Asn Tyr Val His
225 230 235 240
His Asp Gly Phe Met Lys Leu Pro Ser Leu Glu Ser Pro Asn Ser Ile
245 250 255
Ser Ser Gln Asn Cys Tyr Gln Pro Met Ile Thr Asp Asn Glu Gly Ser
260 265 270
Ile Thr Asn Gln Met Ser Tyr Pro Leu Asp Pro Gly Leu Asp Asn Trp
275 280 285
Ala Thr Leu Asp Arg Leu Phe Ala Tyr Gln Leu Asn Gly Gln Thr Glu
290 295 300
Thr Ser Arg Gln Leu Pro Cys Ile Asp Pro Thr Ile Thr Tyr Cys Thr
305 310 315 320
Pro Ser Thr Asp Leu His His Asp Leu Arg Leu Pro Thr Leu Arg Ser
325 330 335
Ser Phe Pro Leu Pro Ser Asn Arg Ser Tyr His Gly Thr Gln Asp Tyr
340 345 350
Asn Asn Glu Ile Asp Leu Trp Asn Phe Thr Thr Arg Ser Ser Pro Asp
355 360 365
Thr Leu Cys Gln Leu Ser Asn Thr Gly Ala Met Ser Ile Ser Val Asn
370 375 380
Gly Gln Ser Gln Val Pro Pro Gly Phe Arg Phe His Pro Thr Glu Glu
385 390 395 400
Glu
<210> 297
<211> 422
<212> PRT
<213> 火炬松()
<220>
<223> 松树转录因子NAC次生壁增厚促进因子 (PtNAC)
<400> 297
Met Pro Glu Asp Met Val Asn Leu Ser Ile Asn Gly Gln Ser Gln Val
1 5 10 15
Pro Pro Gly Phe Arg Phe His Pro Thr Glu Glu Glu Leu Leu His Tyr
20 25 30
Tyr Leu Arg Lys Lys Val Ala Tyr Glu Arg Ile Asp Leu Asp Val Ile
35 40 45
Arg Glu Val Asp Leu Asn Lys Leu Glu Pro Trp Asp Ile Gln Glu Lys
50 55 60
Cys Lys Ile Gly Ser Thr Pro Gln Asn Asp Trp Tyr Phe Phe Ser His
65 70 75 80
Lys Asp Lys Lys Tyr Pro Thr Gly Thr Arg Thr Asn Arg Ala Thr Ala
85 90 95
Ala Gly Phe Trp Lys Ala Thr Gly Arg Asp Lys Ile Ile Tyr Ser Gly
100 105 110
Phe Lys Arg Ile Gly Leu Arg Lys Thr Leu Val Phe Tyr Lys Gly Arg
115 120 125
Ala Pro His Gly Gln Lys Ser Asp Trp Ile Met His Glu Tyr Arg Leu
130 135 140
Asp Asp Ser Thr His Glu Thr Asn Val Ser Asn Pro Ile Gly Glu Ala
145 150 155 160
Ile Pro Glu Glu Gly Trp Val Val Cys Arg Val Phe Arg Lys Lys Asn
165 170 175
Tyr Gln Lys Thr Leu Glu Ser Pro Lys Gly Ser Ser Ser Ser Leu Asp
180 185 190
Ser Lys Ala His Gln Ile Leu Gly Ser Gly Asn Glu Gly Val Leu Asp
195 200 205
Gln Ile Leu Met Tyr Met Gly Arg Thr Cys Lys Met Glu Asn Glu Thr
210 215 220
Phe Ser Asn Met Asn Ile Ser Asn Asn Ser Asn Ser Ser Ser Leu Arg
225 230 235 240
Phe Leu Ser Asn Asn Cys Ile Ser Glu Gly Leu His Glu Arg Phe Met
245 250 255
His Leu Pro Arg Leu Glu Ser Pro Thr Leu Pro Ser Ile Pro Ile Arg
260 265 270
Ser Pro Ser Phe Asp Gln Asp Arg Ser Phe Ile Ser Cys Tyr His Gln
275 280 285
Ser Tyr Asp Glu Met Leu Thr Glu Asn Glu Pro Ser Ser Pro Asn Gln
290 295 300
Val Gly Asn Gly Ile Phe Asp Met Thr Ser Ser Ser Met Thr Asp His
305 310 315 320
Asp Ser Lys Ser Gly Gln Leu Asn Asp Trp Val Thr Leu Asp Arg Leu
325 330 335
Val Ala Ser Gln Leu Asn Gly His Glu Val Glu Thr Ser Lys Gln Leu
340 345 350
Ser Cys Phe Ser Thr Asp Pro Asn Ala Ser Phe Gly Leu Ser Pro Asp
355 360 365
Asp Gly Met Gln Leu Ser His Lys Gln His Ser His Gly Ser Ser Ser
370 375 380
Ser Asn Ile Gln Ala Asn Ser Ser His Val Tyr Ser Asn Glu Asn Asp
385 390 395 400
Leu Trp Ser Leu Thr Lys Ser Ser Ser Pro Ser Ser Ser Ser Asp Pro
405 410 415
Leu Cys His Leu Ser Val
420
<210> 298
<211> 428
<212> PRT
<213> 两色高梁()
<220>
<223> 高粱转录因子NAC次生壁增厚促进因子 (SbNST)
<400> 298
Met Ser Ile Ser Val Asn Gly Gln Ser Val Val Pro Pro Gly Phe Arg
1 5 10 15
Phe His Pro Thr Glu Glu Glu Leu Leu Thr Tyr Tyr Leu Lys Lys Lys
20 25 30
Val Ala Ser Glu Arg Ile Asp Leu Asp Val Ile Arg Asp Val Asp Leu
35 40 45
Asn Lys Leu Glu Pro Trp Asp Ile Gln Glu Lys Cys Arg Ile Gly Ser
50 55 60
Gly Pro Gln Asn Asp Trp Tyr Phe Phe Ser His Lys Asp Lys Lys Tyr
65 70 75 80
Pro Thr Gly Thr Arg Thr Asn Arg Ala Thr Ala Ala Gly Phe Trp Lys
85 90 95
Ala Thr Gly Arg Asp Lys Ala Ile Tyr Ala Ser Gly Ala Arg Arg Ile
100 105 110
Gly Met Arg Lys Thr Leu Val Phe Tyr Lys Gly Arg Ala Pro His Gly
115 120 125
Gln Lys Ser Asp Trp Ile Met His Glu Tyr Arg Leu Glu Pro Ala Leu
130 135 140
Asp Val Asp Ala Ala Ala Gly Ser Ala Ser Ala His His Ala Ala Ala
145 150 155 160
Gly Ala Ala Ala Asp His His Pro Tyr Tyr Thr Ser Ser Ser Pro Pro
165 170 175
Ala Leu Pro Thr Ala Ile Arg Gly Ala Ala Gly Asp Gln Gln Ala Ala
180 185 190
Gln Glu Gln Glu Gly Trp Val Ile Cys Arg Val Phe Lys Lys Lys Asn
195 200 205
Leu Val His His Gly Gln Ser Ser Gly Gly Gly Val Thr Ala Ala Gly
210 215 220
Ser Lys Met Ala Ser Ala Ala Ala Pro Met Glu Gly Ser Pro Ser His
225 230 235 240
Cys Ser Ser Val Thr Val Ile Ser Asp His Thr Met Asn Lys His Gln
245 250 255
Ala Gln Ala Met Leu Gln His Ser Ala Ser Asp Asp Asp Ala Leu Asp
260 265 270
His Ile Leu Gln Tyr Met Gly Gly Gly Gly Gly Lys Gln Pro Asp Thr
275 280 285
Lys Pro Val Leu Leu Asp His His His His His His Leu Ala Ala Ala
290 295 300
Ala Thr Thr Thr Thr Thr Ala Cys Ser Ala Gly Gly Ala Gly Leu Tyr
305 310 315 320
Gly Lys Phe Met Lys Leu Pro Pro Leu Glu His Ala Gly Gly Gly Gly
325 330 335
Gly Leu Leu Pro Ser Pro Ala Gly Ala Cys Asp Tyr Gly Ala Ala Asp
340 345 350
Ala Ser Gly Ile Ala Asp Trp Asp Ala Leu Asp Arg Leu Ala Ala Tyr
355 360 365
Glu Leu Asn Gly Leu Ser Asp Ala Ser Lys Asn Met Ser Ala Phe Phe
370 375 380
Asp Glu Pro Ser Ala Thr Ala Ala Phe Ser Ser Ser Ser Ser Ser Val
385 390 395 400
His Ala Ala Ala Val Asp Gly Asp Leu Trp Ser Leu Ala Arg Ser Val
405 410 415
Ser Ala Leu His Ala Asp Leu Thr Met Asn Asn Val
420 425
<210> 299
<211> 392
<212> PRT
<213> 西加云杉()
<220>
<223> 云杉转录因子NAC次生壁增厚促进因子 (PsNST)
<400> 299
Met Ser Leu Ser Val Asn Gly Gln Ser Arg Val Pro Pro Gly Phe Arg
1 5 10 15
Phe His Pro Thr Glu Glu Glu Leu Leu Tyr Tyr Tyr Leu Lys Lys Lys
20 25 30
Val Ser Phe Glu Lys Ile Asp Leu Asp Val Ile Arg Asp Val Asp Leu
35 40 45
Asn Lys Leu Glu Pro Trp Asp Ile Gln Glu Arg Cys Lys Ile Gly Ser
50 55 60
Thr Pro Gln His Asp Trp Tyr Phe Phe Ser His Lys Asp Lys Lys Tyr
65 70 75 80
Pro Thr Gly Thr Arg Thr Asn Arg Ala Thr Ala Ala Gly Phe Trp Lys
85 90 95
Ala Thr Gly Arg Asp Lys Ala Ile His Thr Asn Leu Lys Lys Ile Gly
100 105 110
Met Arg Lys Thr Leu Val Phe Tyr Lys Gly Arg Ala Pro His Gly Gln
115 120 125
Lys Thr Asp Trp Ile Met His Glu Tyr Arg Leu Asp Asp Ala Glu Tyr
130 135 140
Pro Gln Ala Asn Thr Phe Asn Glu Leu Gln Glu Asp Gly Trp Val Val
145 150 155 160
Cys Arg Val Phe Lys Lys Arg Asn His His Lys Ser Gln Glu Ser Ala
165 170 175
Glu Leu Ser Pro Lys Tyr Gly Ser Lys Gln Phe Ser Asn Asn Lys Ile
180 185 190
Leu Ser Asp Ser Asp Cys Pro Ser Asp Asn Leu Thr Asp Pro Leu His
195 200 205
Tyr Thr His Gln Leu Asn Met Cys Lys Gln Glu Leu Glu Met Gln Gln
210 215 220
Tyr Ala Phe Pro His Asp Gln Phe Met Gln Leu Pro Gln Leu Glu Ser
225 230 235 240
Pro Lys Ile Pro Thr Cys Asn Asn Pro Pro Ser Ser Gly Ile Lys Arg
245 250 255
Phe Phe Pro Asp Ser Arg Ser Gly Phe Phe Gln Leu Glu Asn Ile Thr
260 265 270
Ser Ser Lys Arg Pro Cys Ser Tyr Pro Met Asn Leu Asp Met Thr Glu
275 280 285
Leu Glu Gly Ser Thr Val Thr Thr His Asp His Met Thr Asp Phe Lys
290 295 300
Pro Ala Glu Glu Val Gln Asn Leu Gln Asp Trp Thr Val Leu Asp Arg
305 310 315 320
Leu Val Ala Ser His Leu Asn Gly Gln Glu Ile Glu Thr Pro Lys Leu
325 330 335
Gly Ile Arg Ser Tyr Gly Ala Pro Asn Ser Ser Ser Asp His Gln Ala
340 345 350
Leu Val Asp Ala Ala Thr Val Ser Gly Asp Gln Leu Thr Leu Leu Pro
355 360 365
Gln Leu Arg Ile Asn Arg Leu Asn Pro His Asp Tyr Ala Ser Cys Glu
370 375 380
Ile Asp Leu Trp Asn Phe Val Lys
385 390
<210> 300
<211> 400
<212> PRT
<213> 苹果()
<220>
<223> 苹果转录因子NAC次生壁增厚促进因子 (AppleT)
<400> 300
Met Ser Asp Asp Gln Met Ser Leu Ser Ile Asn Gly Gln Ser Gln Val
1 5 10 15
Pro Pro Gly Phe Arg Phe His Pro Thr Glu Glu Glu Leu Leu His Tyr
20 25 30
Tyr Leu Arg Lys Lys Val Ala Phe Glu Arg Ile Asp Leu Asp Val Ile
35 40 45
Arg Glu Val Asp Leu Asn Lys Leu Glu Pro Trp Asp Ile Gln Glu Lys
50 55 60
Cys Lys Ile Gly Ser Thr Pro Gln Asn Asp Trp Tyr Phe Phe Ser His
65 70 75 80
Lys Asp Lys Lys Tyr Pro Thr Gly Thr Arg Thr Asn Arg Ala Thr Thr
85 90 95
Ala Gly Phe Trp Lys Ala Thr Gly Arg Asp Lys Ile Ile Tyr Ser Gly
100 105 110
Phe Lys Arg Ile Gly Leu Arg Lys Thr Leu Val Phe Tyr Lys Gly Arg
115 120 125
Ala Pro His Gly Gln Lys Ser Asp Trp Ile Met His Glu Tyr Arg Leu
130 135 140
Asp Glu Ser Asn Thr His Glu Thr Thr Val Ser Ser Ser Met Gly Glu
145 150 155 160
Ser Met Thr Glu Glu Gly Trp Val Val Cys Arg Val Phe Lys Lys Lys
165 170 175
Asn Tyr Gln Lys Ala Leu Glu Ser Pro Lys Ala Ser Phe Ser Met Asp
180 185 190
Ser Ser Asn Asn Gln Met His Gly Ser Arg Asn Asp Gly Val Leu Asp
195 200 205
Gln Ile Leu Met Tyr Met Gly Arg Thr Thr Cys Lys Leu Glu Asn His
210 215 220
Asp Gln Ser Leu Thr Met Asn Asn Ile Ser Glu Arg Phe Met His Leu
225 230 235 240
Pro Arg Leu Glu Ser Pro Thr Leu Pro Asn Leu Pro Thr Phe Asp Gln
245 250 255
Glu Arg Ser Phe Lys Ala Cys Tyr Ser Ala Ile Asp Asp Met Phe Ile
260 265 270
Glu Thr Glu Pro Ser Ser Thr Asn Gln Pro Ser Asn Gly Cys His Asn
275 280 285
Asn Asp Leu Val Asp Asp His Glu Tyr Pro Lys Thr Arg Leu Asn Asp
290 295 300
Trp Ala Thr Leu Asp Arg Leu Val Ala Ser Gln Leu Gly Gln Leu Asn
305 310 315 320
Gly Gln Asp Gln Glu Thr Pro Lys His Leu Ser Cys Phe Gly Asp Pro
325 330 335
Asn Met Ala Phe Cys Ser Ser Pro His Pro Asn Asp Gln Asp Asn Asp
340 345 350
Val Gln Leu Ser Tyr Pro Tyr Leu Arg Thr Ser Arg Ser Ser Asp His
355 360 365
Gln Ser Glu Val Tyr Asn Asn Glu Asn Asp Leu Trp Asn Phe Thr Lys
370 375 380
Ser Ser Ser Ser Pro Ser Ser Ser Asp Pro Leu Cys His Leu Ser Val
385 390 395 400
<210> 301
<211> 396
<212> PRT
<213> 水稻()
<220>
<223> 稻转录因子NAC次生壁增厚促进因子 (OsNST)
<400> 301
Met Ser Ile Ser Val Asn Gly Gln Ser Val Val Pro Pro Gly Phe Arg
1 5 10 15
Phe His Pro Thr Glu Glu Glu Leu Leu Thr Tyr Tyr Leu Lys Lys Lys
20 25 30
Val Ala Ser Glu Arg Ile Asp Leu Asp Val Ile Arg Asp Val Asp Leu
35 40 45
Asn Lys Leu Glu Pro Trp Asp Ile Gln Glu Arg Cys Arg Ile Gly Ser
50 55 60
Gly Pro Gln Asn Asp Trp Tyr Phe Phe Ser His Lys Asp Lys Lys Tyr
65 70 75 80
Pro Thr Gly Thr Arg Thr Asn Arg Ala Thr Ala Ala Gly Phe Trp Lys
85 90 95
Ala Thr Gly Arg Asp Lys Ala Ile Tyr Ser Ser Ser Asn Arg Ile Gly
100 105 110
Met Arg Lys Thr Leu Val Phe Tyr Lys Gly Arg Ala Pro His Gly Gln
115 120 125
Lys Ser Asp Trp Ile Met His Glu Tyr Arg Leu Asp Asp Pro Ser Ser
130 135 140
Ala Ser Ala Ser Val Ser Val Asn Leu Pro Ser Tyr Tyr Ser Ser Ser
145 150 155 160
Ser Ser Ser Ser Ser Pro Met His Gly Val Ala Gly Asp Gln Gly Ala
165 170 175
Gln Glu Glu Gly Trp Val Ile Cys Arg Val Phe Lys Lys Lys Asn Leu
180 185 190
Val His His Gly Gly Gly Ser Ala Ala Ala Ser His His Ala Ala Ala
195 200 205
Lys Leu Ala Ala Ala Ala Met Glu Gly Ser Pro Ser Asn Cys Ser Thr
210 215 220
Val Thr Val Ser Asp His Val Lys Ala Gln Met Leu His Ser Ser Ala
225 230 235 240
Ser Asp Asp Ala Leu Asp His Ile Leu Gln Tyr Met Gly Arg Ser Gly
245 250 255
Leu Gln Ala Gly Asp Gln Ala Gly Gly Asp Val Gly Val Val Gly Gly
260 265 270
Ser Gly Gly Gly Ala Gly Ala Ala Pro Gln Tyr Ala Ser Val Arg Gln
275 280 285
Val His Glu Ala Ser Ala Ala Arg Gly Pro Ser Pro Ala Ala Trp Ala
290 295 300
Ser Trp Ala Ala Ala Gly Gly Gly Gly Glu Tyr Cys Ser Ala Ala Asp
305 310 315 320
Ala Ser Gly Ile Ala Asp Trp Asp Thr Leu Asp Arg Leu Ala Ala Ser
325 330 335
Tyr Glu Leu Asn Gly Ala Leu Ser Asp Val Ala Ser Gly Lys Asn Met
340 345 350
Ala Gly Phe Phe Asp Val Val Asp Gln Pro Ala Gly Ala Ala Ala Phe
355 360 365
Ser Ser Gly Asp Gly Asp Leu Trp Ser Leu Ala Arg Ser Val Ser Ser
370 375 380
Ser Leu His Ala Asp Leu Thr Thr Met Asn Asn Val
385 390 395
<210> 302
<211> 433
<212> PRT
<213> 玉米()
<220>
<223> 玉米转录因子NAC次生壁增厚促进因子 (ZmNST)
<400> 302
Met Ser Ile Ser Val Asn Gly Gln Ser Val Val Pro Pro Gly Phe Arg
1 5 10 15
Phe His Pro Thr Glu Glu Glu Leu Leu Thr Tyr Tyr Leu Lys Lys Lys
20 25 30
Val Ala Ser Glu Arg Ile Asp Leu Asp Val Ile Arg Asp Val Asp Leu
35 40 45
Asn Lys Leu Glu Pro Trp Asp Ile Gln Glu Lys Cys Arg Ile Gly Ser
50 55 60
Gly Pro Gln Asn Asp Trp Tyr Phe Phe Ser His Lys Asp Lys Lys Tyr
65 70 75 80
Pro Thr Gly Thr Arg Thr Asn Arg Ala Thr Ala Ala Gly Phe Trp Lys
85 90 95
Ala Thr Gly Arg Asp Lys Ala Ile Tyr Ala Ser Pro Gly Ala Arg Arg
100 105 110
Ile Gly Met Arg Lys Thr Leu Val Phe Tyr Lys Gly Arg Ala Pro His
115 120 125
Gly Gln Lys Ser Asp Trp Ile Met His Glu Tyr Arg Leu Glu Ala Pro
130 135 140
Val Asp Ala Gly Ala Gly Ala Ala His His Leu Leu Leu Pro Ala Ala
145 150 155 160
Glu His Pro Pro Tyr Tyr Thr Ser Pro Pro Gln Ala Pro Ser Ser Thr
165 170 175
Thr Thr Ala Thr Ile Arg Gly Ala Ala Gly Asp Gln Ala Ala Gln Glu
180 185 190
Gln Glu Gly Trp Val Ile Cys Arg Val Phe Lys Lys Lys Asn Leu Val
195 200 205
His His Gly Gln Ser Ser Gly Val Lys Gln Gln Ala Glu Gly Asp Asp
210 215 220
His Ala Ala Ser His Thr Ala Ala Ala Ala Ala His Met Asp Glu Ser
225 230 235 240
Ser Pro Ser Gln Cys Ser Ser Val Thr Val Ile Ser Asp His Val His
245 250 255
Ala Asn Val Asn Asp Lys Gln Gln Gln Ala Gln Ala Ser Leu Leu Met
260 265 270
Met His Thr His His Ser Ala Ser Ser Asp Asp Asp Ala Leu Asp His
275 280 285
Ile Leu Gln Gln Tyr Met Gly Gly Gly Arg Gln Ala Pro Ala Pro Asp
290 295 300
Thr Lys Pro Ala Leu Leu Glu Leu Asp His Arg Leu His His His His
305 310 315 320
Leu Ala Ala Ala Pro Thr Asn Thr Arg Ala Ala Ala Gly Phe Tyr Gly
325 330 335
Lys Phe Met Lys Leu Pro Pro Leu Glu His Ala Gly Leu Pro Pro Ser
340 345 350
Pro Pro Pro Gly Ala Arg Glu Tyr Gly Ala Ala Ala Ala Ala Gly Trp
355 360 365
Asp Asp Asp Asp Asp Ala Leu Asp Arg Leu Ala Ala Tyr Asp His Leu
370 375 380
Asn Gly Leu Ser Asn Asp Ala Ser Lys Asn Met Ala Ala Phe Phe Asp
385 390 395 400
Val Glu Pro Ser Ala Ala Ala Ala Ala Val Asp Gly Asp Leu Trp Ser
405 410 415
Leu Ala Arg Ser Val Ser Ala Leu His Ala Asp Leu Thr Met Asn Asn
420 425 430
Val
<210> 303
<211> 376
<212> PRT
<213> 江南卷柏()
<220>
<223> 卷柏转录因子NAC次生壁增厚促进因子1 (SmNST1)
<400> 303
Met Ser Ser Ser Ile Gln Leu Ser Gly Thr Ser Ser Val Pro Pro Gly
1 5 10 15
Phe Arg Phe His Pro Thr Asp Glu Glu Leu Val Gly Tyr Tyr Leu Ala
20 25 30
Lys Lys Val Ala Ala Ser Lys Ile Asp Leu Asp Val Ile Arg Glu Ile
35 40 45
Asp Leu Tyr Lys Leu Glu Pro Trp Asp Leu Gln Glu Arg Cys Arg Ile
50 55 60
Gly Ser Ala Glu Gln His Glu Trp Tyr Phe Phe Ser His Lys Asp Lys
65 70 75 80
Lys Tyr Pro Thr Gly Thr Arg Thr Asn Arg Ala Thr Gly Ala Gly Phe
85 90 95
Trp Lys Ala Thr Gly Arg Asp Lys Pro Ile Phe Ala Arg Val Ser Lys
100 105 110
Leu Ile Gly Met Arg Lys Thr Leu Val Phe Tyr Lys Gly Arg Ala Pro
115 120 125
Asn Gly Gln Lys Ser Asp Trp Ile Met His Glu Tyr Arg Leu Glu Asp
130 135 140
Asp Ile Asn Asn Thr Ala Thr Asn Glu Glu Gly Trp Val Val Cys Arg
145 150 155 160
Val Phe Arg Lys Arg Asn Val Val Lys Lys Pro Ser Ser Asp Phe Asp
165 170 175
Gly Ala Cys Cys Tyr Asp Pro Glu Glu Asn Met Asp Lys Met Tyr Ser
180 185 190
Ser Asn Ser Ser Phe Val Lys Gln Glu Leu Glu Ser Ala Gly Ile Tyr
195 200 205
Phe Pro Phe Ala Phe Asp His Ser Leu Pro His Leu Glu Ser Pro Lys
210 215 220
Arg Ala Ser Pro Met Ser Tyr Asn Pro Pro Pro Glu Asp His Glu His
225 230 235 240
His His Asp Leu Ile Lys Ala Ser Ser Tyr Ile Asp Asn Glu Leu Glu
245 250 255
Glu Leu Val His Lys Thr Phe Ser Gly Gly Gly Thr Gln Ala Pro Ala
260 265 270
Glu Thr Ser Phe His Gly Phe Ser Asp Glu Met Val Pro Pro Ser Trp
275 280 285
Glu Asp Pro Glu Arg Met Leu Ser Ser Gln Val Ala Gly Val His Glu
290 295 300
Asp Glu Phe Ser Lys Glu Ser Arg Leu Gly Asn Leu Asp Ser Leu Val
305 310 315 320
Glu Arg Met Asn Arg Gly Gln Gln Leu Gln Gln Pro Asp Tyr Ser Tyr
325 330 335
Asp Val Asp Leu Trp Gly Phe Thr Arg Tyr Leu Pro Ile Gln Phe Ala
340 345 350
Gly Phe Cys Tyr Ala Leu Leu Ser Val Ser Leu Pro Ser Val Val Ser
355 360 365
Trp Ile Ser Glu Arg Ala Trp Lys
370 375
<210> 304
<211> 177
<212> PRT
<213> 人工序列()
<220>
<223> 合成的转录因子shine (SHN1, WIN1) 共有序列（大多数）
<220>
<221> 变体
<222> (68)..(.68)
<223> Xaa = Asn, Ser, Ala或Gln
<220>
<221> 变体
<222> (69)..(.69)
<223> Xaa = Thr, Asn, Gly, Pro或Ser
<220>
<221> 变体
<222> (70)..(.70)
<223> Xaa = Glu, Asp, His或Gly
<220>
<221> 变体
<222> (71)..(.71)
<223> Xaa = Lys, Ser, Pro, Leu, Val, Ala或Thr
<220>
<221> 变体
<222> (72)..(.72)
<223> Xaa = Thr, Glu, Asn, Lys, Gln, Asp, Pro或Val
<220>
<221> 变体
<222> (73)..(.73)
<223> Xaa = Asp, Glu, Ser, Thr, Gln, Ala或Pro
<220>
<221> 变体
<222> (75)..(.75)
<223> Xaa = Ser, Asp, Thr, Arg或Gly
<220>
<221> 变体
<222> (99)..(.99)
<223> Xaa = Ser, Asp, Ala, Val, Phe, Gln或Thr
<220>
<221> 变体
<222> (125)..(.125)
<223> Xaa = Ser, Glu, Gln, Pro, Lys, Ala, Thr或Arg
<220>
<221> 变体
<222> (144)..(.144)
<223> Xaa = Lys, Leu, Glu, Gln, Ser, Asp, Ala或Val
<220>
<221> 变体
<222> (157)..(.157)
<223> Xaa = Leu, Ile, Glu, Gly, Val, Met, Lys或Cys
<220>
<221> UNSURE
<222> (68)..(68)
<223> The 'Xaa' at location 68 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (69)..(69)
<223> The 'Xaa' at location 69 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (70)..(70)
<223> The 'Xaa' at location 70 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (71)..(71)
<223> The 'Xaa' at location 71 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (72)..(72)
<223> The 'Xaa' at location 72 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (73)..(73)
<223> The 'Xaa' at location 73 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (75)..(75)
<223> The 'Xaa' at location 75 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (99)..(99)
<223> The 'Xaa' at location 99 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (125)..(125)
<223> The 'Xaa' at location 125 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (144)..(144)
<223> The 'Xaa' at location 144 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (157)..(157)
<223> The 'Xaa' at location 157 stands for Gln, Arg, Pro, or Leu.
<400> 304
Met Val Gln Ser Lys Lys Phe Arg Gly Val Arg Gln Arg His Trp Gly
1 5 10 15
Ser Trp Val Ser Glu Ile Arg His Pro Leu Leu Lys Arg Arg Val Trp
20 25 30
Leu Gly Thr Phe Glu Thr Ala Glu Glu Ala Ala Arg Ala Tyr Asp Glu
35 40 45
Ala Ala Ile Leu Met Ser Gly Arg Asn Ala Lys Thr Asn Phe Pro Val
50 55 60
Pro Arg Asn Xaa Xaa Xaa Xaa Xaa Xaa Ala Xaa Gly Ser Ser Ser Ser
65 70 75 80
Ser Ser Lys Ser Leu Ser Gln Ile Leu Ser Ala Lys Leu Arg Lys Cys
85 90 95
Cys Lys Xaa Pro Ser Pro Ser Leu Thr Cys Leu Arg Leu Asp Thr Glu
100 105 110
Asn Ser His Ile Gly Val Trp Gln Lys Arg Ala Gly Xaa Arg Ser Asp
115 120 125
Ser Asn Trp Val Met Thr Val Glu Leu Gly Lys Lys Val Ser Ser Xaa
130 135 140
Ser Gln Ser Thr Ser Pro Thr Thr Ser Ser Glu Glu Xaa Met Asp Glu
145 150 155 160
Glu Glu Arg Ile Ala Leu Gln Met Ile Glu Glu Leu Leu Asn Ser Ser
165 170 175
Ser
<210> 305
<211> 122
<212> PRT
<213> 拟南芥()
<220>
<223> 转录因子shine (AtSHN2), At5g11190
<400> 305
Met Val His Ser Arg Lys Phe Arg Gly Val Arg Gln Arg Gln Trp Gly
1 5 10 15
Ser Trp Val Ser Glu Ile Arg His Pro Leu Leu Lys Arg Arg Val Trp
20 25 30
Leu Gly Thr Phe Glu Thr Ala Glu Ala Ala Ala Arg Ala Tyr Asp Gln
35 40 45
Ala Ala Leu Leu Met Asn Gly Gln Asn Ala Lys Thr Asn Phe Pro Val
50 55 60
Val Lys Ser Glu Glu Gly Ser Asp His Val Lys Asp Val Asn Ser Pro
65 70 75 80
Leu Met Leu Gly Leu Thr Thr Met Asn Lys Gln Asn Val Glu Lys Glu
85 90 95
Glu Glu Glu Glu Glu Ala Ile Ile Ser Asp Glu Asp Gln Leu Ala Met
100 105 110
Glu Met Ile Glu Glu Leu Leu Asn Trp Ser
115 120
<210> 306
<211> 189
<212> PRT
<213> 拟南芥()
<220>
<223> 转录因子shine (AtSHN3), At5g25390
<400> 306
Met Val His Ser Lys Lys Phe Arg Gly Val Arg Gln Arg Gln Trp Gly
1 5 10 15
Ser Trp Val Ser Glu Ile Arg His Pro Leu Leu Lys Arg Arg Val Trp
20 25 30
Leu Gly Thr Phe Asp Thr Ala Glu Thr Ala Ala Arg Ala Tyr Asp Gln
35 40 45
Ala Ala Val Leu Met Asn Gly Gln Ser Ala Lys Thr Asn Phe Pro Val
50 55 60
Ile Lys Ser Asn Gly Ser Asn Ser Leu Glu Ile Asn Ser Ala Leu Arg
65 70 75 80
Ser Pro Lys Ser Leu Ser Glu Leu Leu Asn Ala Lys Leu Arg Lys Asn
85 90 95
Cys Lys Asp Gln Thr Pro Tyr Leu Thr Cys Leu Arg Leu Asp Asn Asp
100 105 110
Ser Ser His Ile Gly Val Trp Gln Lys Arg Ala Gly Ser Lys Thr Ser
115 120 125
Pro Asn Trp Val Lys Leu Val Glu Leu Gly Asp Lys Val Asn Ala Arg
130 135 140
Pro Gly Gly Asp Ile Glu Thr Asn Lys Met Lys Val Arg Asn Glu Asp
145 150 155 160
Val Gln Glu Asp Asp Gln Met Ala Met Gln Met Ile Glu Glu Leu Leu
165 170 175
Asn Trp Thr Cys Pro Gly Ser Gly Ser Ile Ala Gln Val
180 185
<210> 307
<211> 206
<212> PRT
<213> 毛果杨()
<220>
<223> 转录因子shine (PtSHN1)
<400> 307
Met Val Gln Ser Lys Lys Phe Arg Gly Val Arg Gln Arg His Trp Gly
1 5 10 15
Ser Trp Val Ser Glu Ile Arg His Pro Leu Leu Lys Arg Arg Val Trp
20 25 30
Leu Gly Thr Phe Glu Thr Ala Glu Glu Ala Ala Arg Ala Tyr Asp Gln
35 40 45
Ala Ala Ile Leu Met Ser Gly Arg Asn Ala Lys Thr Asn Phe Pro Ile
50 55 60
Pro Gln Thr Ser Asn Glu Glu Asp Pro Lys Ser Ser Asp Glu Ala Ser
65 70 75 80
Leu Pro Thr Pro Pro Asn Gly Leu Ser Glu Ile Leu His Ala Lys Leu
85 90 95
Arg Lys Cys Ser Lys Ala Pro Ser Pro Ser Met Thr Cys Leu Arg Leu
100 105 110
Asp Thr Glu Asn Ser Leu Ile Gly Val Trp Gln Lys Arg Ala Gly Glu
115 120 125
Arg Ser Asp Ser Asn Trp Val Met Arg Val Gln Leu Gly Gln Arg Glu
130 135 140
Ser Gln Val Ser Glu Ser Thr Leu Pro Leu Pro Gln Ser Ser Gly Gly
145 150 155 160
Val Ser Glu Pro Glu Leu Arg Ala Glu Met Gly Glu Asp Glu Arg Ile
165 170 175
Ala Leu Gln Met Ile Glu Glu Leu Leu Asn Arg Asn Cys Pro Ser Pro
180 185 190
Ser Phe Gly Val Gln Asp His Gly Asp Gly Ser Leu Phe Leu
195 200 205
<210> 308
<211> 208
<212> PRT
<213> 毛果杨()
<220>
<223> 转录因子shine (PtSHN2)
<400> 308
Met Val Pro Ser Lys Lys Phe Arg Gly Val Arg Gln Arg Arg Trp Gly
1 5 10 15
Ser Trp Val Ser Glu Ile Arg His Pro Leu Val Lys Arg Arg Val Trp
20 25 30
Leu Gly Thr Phe Glu Thr Ala Glu Glu Ala Ala Arg Ala Tyr Asp Gln
35 40 45
Ala Ala Ile Leu Met Ser Gly Arg Asn Ala Lys Thr Asn Phe Pro Met
50 55 60
Pro Gln Thr Ser Asn Glu Asp Asp Pro Lys Ser Ser Asp His Gln Pro
65 70 75 80
Ser Leu Thr Thr Pro Pro Asn Gly Leu Ser Gln Ile Leu His Ala Lys
85 90 95
Leu Arg Lys Cys Ser Lys Ala Pro Ser Pro Ser Met Thr Cys Leu Arg
100 105 110
Leu Asp Ala Glu Asn Ser Ile Gly Val Trp Gln Gln Arg Ala Gly Gln
115 120 125
Arg Ser Asp Ser Asn Trp Val Met Thr Val Gln Leu Gly Lys Arg Asp
130 135 140
Glu Ser Gln Val Ser Glu Ser Ala Leu Pro Leu Pro Asp Gln Ser Pro
145 150 155 160
Gly Gly Ile Ser Gly Pro Glu Trp Arg Glu Glu Met Asp Lys Glu Glu
165 170 175
Arg Val Ala Leu Gln Met Val Glu Glu Leu Leu Asn Arg Asn Cys Pro
180 185 190
Ser Pro Pro Phe Gly Val Gln Asp His Asp Asp Asp Ser Phe Phe Leu
195 200 205
<210> 309
<211> 179
<212> PRT
<213> 毛果杨()
<220>
<223> 转录因子shine (PtSHN3)
<400> 309
Met Val Gln Ser Lys Lys Phe Arg Gly Val Arg Gln Arg His Trp Gly
1 5 10 15
Ser Trp Val Ser Glu Ile Arg His Pro Leu Leu Lys Arg Arg Val Trp
20 25 30
Leu Gly Thr Phe Asp Thr Ala Glu Glu Ala Ala Arg Ala Tyr Asp Glu
35 40 45
Ala Ala Ile Leu Met Ser Gly Arg Asn Ala Lys Thr Asn Phe Pro Val
50 55 60
Val Ala Asn Gln Thr Arg Asn Gly Gln Asn Ser Pro Ser Ser Ser Ser
65 70 75 80
Ala Leu Ser Ala Lys Leu Arg Lys Tyr Cys Arg Ser Pro Tyr Pro Ser
85 90 95
Leu Thr Cys Leu Arg Leu Asp Ala Glu Asn Cys His Ile Gly Val Trp
100 105 110
Gln Lys Arg Ala Gly Pro Arg Ser Val Ser Asn Trp Ile Met Thr Val
115 120 125
Glu Leu Gly Lys Lys Asp Gly Arg Gln Ala Pro Glu Gln Lys Ile Leu
130 135 140
Ile Ser Asp Thr Ser Asp Met Ala Gly Gln Glu Gly Gly Ser Asp Asp
145 150 155 160
Gly Pro Asp Asp Glu Glu Arg Val Ala Leu Gln Met Ile Glu Glu Leu
165 170 175
Leu Asn Arg
<210> 310
<211> 178
<212> PRT
<213> 毛果杨()
<220>
<223> 转录因子shine (PtSHN4)
<400> 310
Met Val Gln Ser Lys Lys Phe Arg Gly Val Arg Gln Arg Gln Trp Gly
1 5 10 15
Ser Trp Val Ser Glu Ile Arg His Pro Leu Leu Lys Arg Arg Val Trp
20 25 30
Leu Gly Thr Phe Glu Thr Ala Glu Ala Ala Ala Arg Ala Tyr Asp Gln
35 40 45
Ala Ala Ile Leu Met Asn Gly Gln Asn Ala Lys Thr Asn Phe Pro Thr
50 55 60
Ser His Leu Asp Gln Asp Thr Asn Leu Gly Lys Asp Asn Asn Ser Pro
65 70 75 80
Leu Pro Ala Lys Ala Leu Ala Glu Leu Leu Asn Ser Lys Leu Arg Lys
85 90 95
Cys Cys Gly Lys Asp Pro Ser Pro Ser Leu Thr Cys Leu Arg Leu Asp
100 105 110
Asn Asp Asn Ser His Ile Gly Val Trp Gln Lys Lys Ala Gly Ser Arg
115 120 125
Ser Ser Ser Asn Trp Val Met Lys Val Glu Leu Gly Asn Tyr Asn Lys
130 135 140
Lys Thr Glu Ser Ser Pro Thr Val Glu Ile Glu Pro Glu Asn Gly Thr
145 150 155 160
Glu Glu Glu Asp Arg Ile Ala Met Gln Met Ile Glu Glu Leu Leu Asn
165 170 175
Arg Asn
<210> 311
<211> 192
<212> PRT
<213> 毛果杨()
<220>
<223> 转录因子shine (PtSHN5)
<400> 311
Met Val Gln Ser Lys Lys Phe Arg Gly Val Arg Gln Arg Gln Trp Gly
1 5 10 15
Ser Trp Val Ser Glu Ile Arg His Pro Leu Leu Lys Arg Arg Val Trp
20 25 30
Leu Gly Thr Phe Glu Thr Ala Glu Ala Ala Ala Arg Ala Tyr Asp Gln
35 40 45
Ala Ala Ile Leu Met Asn Gly Gln Asn Ala Lys Thr Asn Phe Pro Ala
50 55 60
Ser His Leu Asp Gln Asp Thr Lys Leu Gly Lys Asp Asn Asn Ser Pro
65 70 75 80
Leu Pro Ala Lys Ala Leu Ala Glu Leu Leu Tyr Ser Lys Leu Arg Lys
85 90 95
Cys Cys Gly Lys Asp Pro Ser Pro Ser Leu Thr Cys Leu Arg Leu Asp
100 105 110
Asn Asp Asn Ser His Ile Gly Val Trp Gln Lys Lys Ala Gly Ser Cys
115 120 125
Ser Ser Ser Asn Trp Val Met Arg Val Glu Leu Gly Asn Ser Asn Arg
130 135 140
Lys Ser Thr Gln Val Met Glu Glu Leu Arg Pro Ser Leu Ser Ser Glu
145 150 155 160
Ser Ser Ser Arg Val Glu Ile Glu Pro Glu Ile Asn Gly Thr Asp Glu
165 170 175
Glu Asp Lys Ile Ala Met Gln Met Ile Asp Glu Leu Leu Asn Cys Asn
180 185 190
<210> 312
<211> 214
<212> PRT
<213> 蒺藜状苜蓿()
<220>
<223> 转录因子shine (MtSHN1)
<400> 312
Met Val Gln Ser Lys Lys Phe Arg Gly Val Arg Gln Arg His Trp Gly
1 5 10 15
Ser Trp Val Ser Glu Ile Arg His Pro Leu Leu Lys Arg Arg Val Trp
20 25 30
Leu Gly Thr Phe Glu Thr Ala Glu Glu Ala Ala Arg Ala Tyr Asp Gln
35 40 45
Ala Ala Ile Leu Met Ser Gly Arg Asn Ala Lys Thr Asn Phe Pro Ile
50 55 60
Thr Gln Thr Ser Glu Gly Asp Pro Lys Ser Ile Thr Ser Asn Glu Asn
65 70 75 80
Lys Pro Ser Thr Ser Lys Asp Leu Glu Glu Ile Leu His Ala Lys Leu
85 90 95
Arg Lys Cys Ser Lys Val Pro Ser Pro Ser Met Thr Cys Leu Arg Leu
100 105 110
Asp Thr Glu Asn Ser His Ile Gly Val Trp Gln Lys Arg Ala Gly Lys
115 120 125
Cys Ser Glu Ser Asn Trp Val Met Thr Val Gln Leu Gly Lys Lys Met
130 135 140
Ser Val Thr Gln Asp Ser Gly Ser Ser Ser Ser Ser Val Ala Pro Ser
145 150 155 160
Ser Ala Val Ala Thr Glu Glu Glu Ile Val Arg Gly Glu Ile Asp Glu
165 170 175
Glu Asp Arg Ile Ala Leu Gln Met Ile Glu Glu Leu Leu Asn Asp Lys
180 185 190
Asn Cys Pro Ser Pro Ser Ile Asn Asn Ile Lys Gln Gly Asp Asp Ile
195 200 205
Asp Asn Ser Phe Phe Leu
210
<210> 313
<211> 175
<212> PRT
<213> 蒺藜状苜蓿()
<220>
<223> 转录因子shine (MtSHN2)
<400> 313
Met Val His Ser Lys Lys Phe Arg Gly Val Arg Gln Arg His Trp Gly
1 5 10 15
Ser Trp Val Ser Glu Ile Arg His Pro Leu Leu Lys Arg Arg Val Trp
20 25 30
Leu Gly Thr Phe Glu Thr Ala Glu Glu Ala Ala Lys Ala Tyr Asp Glu
35 40 45
Ala Ala Ile Leu Met Ser Gly Arg Asn Ala Lys Thr Asn Phe Pro Ile
50 55 60
Asn Val Glu Asn Gln Thr Asn Ser Ile Ser Ser Ser Ser Thr Ser Ser
65 70 75 80
Lys Ala Phe Ser Ala Val Leu Ser Ala Lys Leu Arg Lys Cys Cys Lys
85 90 95
Phe Pro Ser Pro Ser Leu Thr Cys Leu Arg Leu Asp Ala Glu Asn Ser
100 105 110
His Ile Gly Val Trp Gln Lys Gly Ala Gly Pro Arg Ser Glu Ser Asn
115 120 125
Trp Ile Met Met Val Glu Leu Glu Arg Lys Lys Ser Ala Ser Val Pro
130 135 140
Glu Lys Ala Lys Pro Glu Glu Leu Ser Lys Asn Gly Leu Asp Asp Glu
145 150 155 160
Gln Lys Ile Ala Leu Gln Met Ile Glu Glu Leu Leu Asn Arg Asn
165 170 175
<210> 314
<211> 196
<212> PRT
<213> 蒺藜状苜蓿()
<220>
<223> 转录因子shine (MtSHN3)
<400> 314
Met Val Lys Ser Lys Lys Phe Arg Gly Val Arg Gln Arg His Trp Gly
1 5 10 15
Ser Trp Val Ser Glu Ile Arg His Pro Leu Leu Lys Arg Arg Val Trp
20 25 30
Leu Gly Thr Phe Glu Thr Ala Glu Glu Ala Ala Arg Ala Tyr Asp Glu
35 40 45
Ala Ala Ile Leu Met Thr Asn Ser Asn Asn Lys Thr Phe Ala Thr Ser
50 55 60
Ser Ser Thr Ser Thr Lys Pro Asn Thr Ser Leu Ser Ala Ile Leu Ser
65 70 75 80
Ala Lys Leu Arg Lys Cys Cys Lys Ser Pro Ser Pro Ser Leu Thr Cys
85 90 95
Leu Arg Leu Asp Thr Glu Asn Ser His Phe Gly Val Trp Gln Lys Arg
100 105 110
Ala Gly Pro Arg Ser Asp Ser Ser Trp Ile Met Met Val Glu Leu Glu
115 120 125
Arg Lys Lys Lys Glu Gln Glu Glu Glu Ser Glu Val Leu Pro Asn Ser
130 135 140
Asp Ser Glu Thr Leu Ala Ser Val Val Asp Asn Glu Asp Ser Glu Lys
145 150 155 160
Ala Val Lys Pro Glu Asn Glu Asp Glu Glu Gly Asn Asp Lys Asn Lys
165 170 175
Gly Leu Asp Glu Glu Gln Arg Ile Ala Leu Gln Met Ile Glu Glu Leu
180 185 190
Leu Asn Arg Asn
195
<210> 315
<211> 208
<212> PRT
<213> 蒺藜状苜蓿()
<220>
<223> 转录因子shine (MtSHN4)
<400> 315
Met Val Gln Gln Thr Lys Lys Phe Arg Gly Val Arg Gln Arg Gln Trp
1 5 10 15
Gly Ser Trp Val Ser Glu Ile Arg His Pro Leu Leu Lys Arg Arg Val
20 25 30
Trp Leu Gly Thr Phe Glu Thr Ala Glu Ala Ala Ala Arg Ala Tyr Asp
35 40 45
Gln Ala Ala Ile Leu Met Asn Gly Gln Ser Ala Lys Thr Asn Phe Pro
50 55 60
Val Thr Lys Asn Gln Gly Glu Glu Val Ala Ser Asp Thr Pro Tyr Asn
65 70 75 80
Gly Gly Gly Gly Asp Asp Ser Phe Leu Ser Pro Lys Ala Leu Ser Glu
85 90 95
Leu Leu Ser Thr Lys Leu Arg Lys Tyr Cys Lys Asp Pro Ser Pro Ser
100 105 110
Leu Thr Cys Leu Arg Leu Asp Asn Asp Asn Ser His Ile Gly Val Trp
115 120 125
Gln Lys Arg Ala Gly Pro His Ser Asp Ser Asn Trp Val Met Arg Val
130 135 140
Glu Leu Gly Gly Lys Lys Lys Thr Ile Glu Ser Glu Glu Ile Gly Ser
145 150 155 160
Lys Gln His Thr Ile Asp Gly Gly Asn Asn Ser Asn Ala Asp Asn Glu
165 170 175
Asn Arg Val Val Val Glu Glu Glu Glu Arg Val Ala Leu Gln Met Ile
180 185 190
Glu Glu Leu Leu Asn Trp Asn Tyr Pro Cys Gly Ser Thr Ser Ser Asn
195 200 205
<210> 316
<211> 197
<212> PRT
<213> 蒺藜状苜蓿()
<220>
<223> 转录因子shine (MtSHN5)
<400> 316
Met Val Gln Arg Asn Lys Phe Arg Gly Val Arg Gln Arg Gln Trp Gly
1 5 10 15
Ser Trp Val Ser Glu Ile Arg His Pro Leu Leu Lys Arg Arg Val Trp
20 25 30
Leu Gly Thr Phe Glu Thr Ala Glu Ala Ala Ala Arg Ala Tyr Asp Gln
35 40 45
Ala Ala Ile Leu Met Asn Gly Lys Asn Ala Lys Thr Asn Phe Pro Ile
50 55 60
Pro Lys Asp Gln Thr Glu Asp Ala Asn Ser Leu Thr Pro Asn Cys Asp
65 70 75 80
Asp Asn Asn Asn Ser Phe His Thr Ser Asn Ala Leu Ser His Leu Leu
85 90 95
Lys Gln Lys Leu Thr Lys Cys Cys Gln Lys Gln Ser Gln Ser Leu Thr
100 105 110
Cys Leu Arg Leu Asp Ala Asp Asn Ser His Ile Gly Val Trp Gln Lys
115 120 125
Gly Ala Gly Ser His Ser Asp Ser Asn Trp Ile Leu Arg Val Glu Leu
130 135 140
Gly Lys Lys His Glu Asp Ser His Glu Ser Asn Tyr Val Ser Ser Ser
145 150 155 160
Glu Lys Ser Ala Pro Asn Asn Ser Thr Ile Val Gly Asp Cys Ala Glu
165 170 175
Lys Asn Gly Ile Glu His Glu Glu Asp Ile Val Thr Met Gln Met Ile
180 185 190
Glu Glu Leu Leu Asn
195
<210> 317
<211> 205
<212> PRT
<213> 水稻()
<220>
<223> 转录因子shine (OsSHN1)
<400> 317
Met Val Gln Pro Lys Lys Lys Phe Arg Gly Val Arg Gln Arg His Trp
1 5 10 15
Gly Ser Trp Val Ser Glu Ile Arg His Pro Leu Leu Lys Arg Arg Val
20 25 30
Trp Leu Gly Thr Phe Glu Thr Ala Glu Glu Ala Ala Arg Ala Tyr Asp
35 40 45
Glu Ala Ala Val Leu Met Ser Gly Arg Asn Ala Lys Thr Asn Phe Pro
50 55 60
Val Gln Arg Asn Ser Thr Gly Asp Leu Ala Thr Ala Ala Asp Gln Asp
65 70 75 80
Ala Arg Ser Asn Gly Gly Ser Arg Asn Ser Ser Ala Gly Asn Leu Ser
85 90 95
Gln Ile Leu Ser Ala Lys Leu Arg Lys Cys Cys Lys Ala Pro Ser Pro
100 105 110
Ser Leu Thr Cys Leu Arg Leu Asp Pro Glu Lys Ser His Ile Gly Val
115 120 125
Trp Gln Lys Arg Ala Gly Ala Arg Ala Asp Ser Asn Trp Val Met Thr
130 135 140
Val Glu Leu Asn Lys Glu Val Glu Pro Thr Glu Pro Ala Ala Gln Pro
145 150 155 160
Thr Ser Thr Ala Thr Ala Ser Gln Val Thr Met Asp Asp Glu Glu Lys
165 170 175
Ile Ala Leu Gln Met Ile Glu Glu Leu Leu Ser Arg Ser Ser Pro Ala
180 185 190
Ser Pro Ser His Gly Glu Gly Glu Gly Ser Phe Val Ile
195 200 205
<210> 318
<211> 225
<212> PRT
<213> 紫短柄草()
<220>
<223> 转录因子shine (BdSHN1)
<400> 318
Met Val Gln Ser Lys Lys Lys Phe Arg Gly Val Arg Gln Arg His Trp
1 5 10 15
Gly Ser Trp Val Ser Glu Ile Arg His Pro Leu Leu Lys Arg Arg Val
20 25 30
Trp Leu Gly Thr Phe Glu Thr Ala Glu Glu Ala Ala Arg Ala Tyr Asp
35 40 45
Glu Ala Ala Ile Leu Met Ser Gly Arg Asn Ala Lys Thr Asn Phe Pro
50 55 60
Val Pro Arg Ser Ala Thr Gly Glu Ile Ile Val Ala Pro Ala Ala Ala
65 70 75 80
Arg Asp Ser Arg Gly Gly Gly Leu Gly Ser Ser Ser Gly Ala Gly Ser
85 90 95
Leu Ser Gln Ile Leu Ser Ala Lys Leu Arg Lys Cys Cys Lys Thr Pro
100 105 110
Ser Pro Ser Leu Thr Cys Leu Arg Leu Asp Thr Glu Lys Ser His Ile
115 120 125
Gly Val Trp Gln Lys Arg Ala Gly Thr Arg Ala Asp Ser Ser Trp Val
130 135 140
Met Thr Val Glu Leu Asn Lys Glu Pro Ala Ala Ala Ala Thr Thr Thr
145 150 155 160
Thr Leu Ser Asp Ser Val Ala Pro Thr Thr Pro Ser Thr Ser Ser Thr
165 170 175
Ser Ala Ser Thr Ala Gly Ser Pro Pro Val Gly Met Asp Asp Glu Glu
180 185 190
Arg Ile Ala Leu Gln Met Ile Glu Glu Leu Leu Gly Gly Ser Ser Pro
195 200 205
Asn Ser Pro Ser His Gly Leu Leu Gln Gly Glu Glu Gly Ser Phe Val
210 215 220
Ile
225
<210> 319
<211> 211
<212> PRT
<213> 紫短柄草()
<220>
<223> 转录因子shine (BdSHN2)
<400> 319
Met Val Gln Pro Lys Lys Lys Phe Arg Gly Val Arg Gln Arg His Trp
1 5 10 15
Gly Ser Trp Val Ser Glu Ile Arg His Pro Leu Leu Lys Arg Arg Val
20 25 30
Trp Leu Gly Thr Phe Glu Thr Ala Glu Glu Ala Ala Arg Ala Tyr Asp
35 40 45
Glu Ala Ala Val Leu Met Ser Gly Arg Asn Ala Lys Thr Asn Phe Pro
50 55 60
Val Gln Arg Ser Ser Thr Gly Asp Pro Ala Pro Ala Ala Gly Arg Asp
65 70 75 80
Val Arg Gly Gly Asn Gly Gly Gly Ser Ser Ser Ser Ser Met Ser Asn
85 90 95
Leu Ser Gln Ile Leu Ser Ala Lys Leu Arg Lys Cys Cys Lys Ala Pro
100 105 110
Ser Pro Ser Leu Thr Cys Leu Arg Leu Asp Pro Glu Lys Ser His Ile
115 120 125
Gly Val Trp Gln Lys Arg Ala Gly Ala Arg Ala Asp Ser Asn Trp Val
130 135 140
Met Thr Val Glu Leu Asn Lys Gly Val Gly Leu Pro Ser Asp Val Glu
145 150 155 160
Ala Gln Ser Thr Ile Ser Thr Ala Thr Thr Ser Ser Ser Val Ser Thr
165 170 175
Met Asp Asp Glu Glu Lys Leu Thr Leu Gln Met Ile Glu Glu Leu Leu
180 185 190
Ser Arg Ser Gly Pro Val Ser Pro Ser His Gly Glu Asp Glu Gly Asp
195 200 205
Phe Val Val
210
<210> 320
<211> 204
<212> PRT
<213> 玉米()
<220>
<223> 转录因子shine (ZmSHN1)
<400> 320
Met Val Gln Pro Lys Lys Phe Arg Gly Val Arg Gln Arg His Trp Gly
1 5 10 15
Ser Trp Val Ser Glu Ile Arg His Pro Leu Leu Lys Arg Arg Val Trp
20 25 30
Leu Gly Thr Phe Glu Thr Ala Glu Glu Ala Ala Arg Ala Tyr Asp Glu
35 40 45
Ala Ala Val Leu Met Ser Gly Arg Asn Ala Lys Thr Asn Phe Pro Ile
50 55 60
Gln Arg Ser Ser Thr Gly Glu Pro Thr Pro Ala Ala Gly Arg Asp Ala
65 70 75 80
Arg Ser Asn Phe Ser Ser Gly Ser Ser Thr Thr Asn Leu Ser Gln Ile
85 90 95
Leu Ser Ala Lys Leu Arg Lys Cys Cys Lys Ala Pro Ser Pro Ser Leu
100 105 110
Thr Cys Leu Arg Leu Asp Pro Glu Lys Ser His Ile Gly Val Trp Gln
115 120 125
Lys Arg Ala Gly Ala Arg Ala Asp Ser Asn Trp Val Met Thr Val Glu
130 135 140
Leu Asn Lys Asp Ala Ala Ser Thr Asp Ala Ala Ser Gln Ser Thr Ser
145 150 155 160
Ala Thr Thr Ala Pro Pro Ala Thr Pro Met Asp Glu Glu Glu Arg Ile
165 170 175
Ala Leu Gln Met Ile Glu Glu Leu Leu Ser Ser Ser Ser Pro Ala Ser
180 185 190
Pro Ser Asn Gly Asp Asp Gln Gly Arg Phe Ile Ile
195 200
<210> 321
<211> 204
<212> PRT
<213> 两色高梁()
<220>
<223> 转录因子shine (SbSHN1)
<400> 321
Met Val Gln Pro Lys Lys Phe Arg Gly Val Arg Gln Arg His Trp Gly
1 5 10 15
Ser Trp Val Ser Glu Ile Arg His Pro Leu Leu Lys Arg Arg Val Trp
20 25 30
Leu Gly Thr Phe Glu Thr Ala Glu Glu Ala Ala Arg Ala Tyr Asp Glu
35 40 45
Ala Ala Val Leu Met Ser Gly Arg Asn Ala Lys Thr Asn Phe Pro Val
50 55 60
Gln Arg Ser Ser Thr Gly Glu Pro Thr Pro Ala Ala Gly Arg Asp Ala
65 70 75 80
His Ser Asn Ala Gly Ser Gly Ser Ser Thr Ala Asn Leu Ser Gln Ile
85 90 95
Leu Ser Ala Lys Leu Arg Lys Cys Cys Lys Ala Pro Ser Pro Ser Leu
100 105 110
Thr Cys Leu Arg Leu Asp Pro Glu Lys Ser His Ile Gly Val Trp Gln
115 120 125
Lys Arg Ala Gly Ala Arg Ala Asp Ser Asn Trp Val Met Thr Val Glu
130 135 140
Leu Asn Lys Gly Ala Ala Ser Thr Asp Ala Ala Ser Gln Ser Thr Ser
145 150 155 160
Ala Thr Thr Ala Pro Pro Ala Thr Pro Met Asp Asp Glu Glu Arg Ile
165 170 175
Ala Leu Gln Met Ile Glu Glu Leu Leu Ser Ser Ser Ser Pro Ala Ser
180 185 190
Pro Ser His Gly Asp Asp Gln Gly Arg Phe Ile Ile
195 200
<210> 322
<211> 235
<212> PRT
<213> 两色高梁()
<220>
<223> 转录因子shine (SbSHN2)
<400> 322
Met Val Gln Ser Lys Lys Lys Phe Arg Gly Val Arg Gln Arg His Trp
1 5 10 15
Gly Ser Trp Val Ser Glu Ile Arg His Pro Leu Leu Lys Arg Arg Val
20 25 30
Trp Leu Gly Thr Phe Glu Thr Ala Glu Glu Ala Ala Arg Ala Tyr Asp
35 40 45
Glu Ala Ala Val Leu Met Ser Gly Arg Asn Ala Lys Thr Asn Phe Pro
50 55 60
Val Pro Arg Thr Ala Thr Gly Glu Leu Ala Pro Val Pro Ala Ala Arg
65 70 75 80
Asp Ala Arg Gly Gly Gly Gly Ser Ser Ser Ala Ala Ala Ala Pro Gly
85 90 95
Gly Gly Thr Ser Asn Leu Ser Gln Ile Leu Ser Ala Lys Leu Arg Lys
100 105 110
Cys Cys Lys Thr Pro Ser Pro Ser Leu Thr Cys Leu Arg Leu Asp Pro
115 120 125
Glu Lys Ser His Ile Gly Val Trp Gln Lys Arg Ala Gly Ala Arg Ala
130 135 140
Asp Ser Ser Trp Val Met Thr Val Gln Leu Asn Lys Asp Val Pro Pro
145 150 155 160
Pro Ala Ser Ser Ser Gly Glu Glu Pro Val Pro Ser Asp Gly Gly Ala
165 170 175
Ala Ala Thr Thr Pro Thr Ser Thr Ser Thr Ser Ser Thr Val Thr Thr
180 185 190
Thr Gly Ser Pro Pro Pro Ala Met Met Met Asp Asp Glu Glu Arg Ile
195 200 205
Ala Leu Gln Met Ile Glu Glu Leu Leu Gly Ser Ser His Ser His Gly
210 215 220
Met Phe Gln Gly Ala Ala Gly Ser Ile Val Ile
225 230 235
<210> 323
<211> 171
<212> PRT
<213> 大麦()
<220>
<223> 转录因子shine (HvSHN1)
<400> 323
Met Val Gln Ser Lys Lys Lys Phe Arg Gly Val Arg Gln Arg His Trp
1 5 10 15
Gly Ser Trp Val Ser Glu Ile Arg His Pro Leu Leu Lys Arg Arg Val
20 25 30
Trp Leu Gly Thr Phe Glu Thr Ala Glu Glu Ala Ala Arg Ala Tyr Asp
35 40 45
Glu Ala Ala Ile Leu Met Ser Gly Arg Asn Ala Lys Thr Asn Phe Pro
50 55 60
Val Pro Arg Ser Ala Asn Gly Glu Ile Ile Val Ala Pro Ala Ala Ala
65 70 75 80
Ala Arg Asp Ile Arg Gly Gly Val Gly Ser Ser Ser Ser Gly Ala Ala
85 90 95
Gly Ala Ser Ser Leu Ser Gln Ile Leu Ser Ala Lys Leu Arg Lys Cys
100 105 110
Cys Lys Thr Pro Ser Pro Ser Leu Thr Cys Leu Arg Leu Asp Thr Glu
115 120 125
Lys Ser His Ile Gly Val Trp Gln Lys Arg Ala Gly Ala Arg Ala Asp
130 135 140
Ser Ser Trp Val Met Thr Val Glu Leu Asn Lys Glu Pro Ala Ala Ala
145 150 155 160
Ala Pro Pro Thr Pro Ser Asp Ser Thr Val Ser
165 170
<210> 324
<211> 209
<212> PRT
<213> 西加云杉()
<220>
<223> 转录因子shine (PsSHN1)
<400> 324
Met Ala Arg Pro Gln Arg Tyr Arg Gly Val Arg Gln Arg His Trp Gly
1 5 10 15
Ser Trp Val Ser Glu Ile Arg His Pro Leu Leu Lys Thr Arg Ile Trp
20 25 30
Leu Gly Thr Phe Glu Thr Ala Glu Asp Ala Ala Arg Ala Tyr Asp Glu
35 40 45
Ala Ala Arg Met Met Cys Gly Pro Arg Ala Arg Thr Asn Phe Pro Phe
50 55 60
Asn Pro Asn Ala Pro Gln Ser Pro Ser Ser Lys Val Leu Ser Ser Thr
65 70 75 80
Leu Thr Ala Lys Leu His Arg Cys Tyr Met Ala Ser Met Gln Gly Pro
85 90 95
Arg Ser Gly Ser Ser Lys Lys Asp Ser Met Ala Arg Ala Asp Lys Asn
100 105 110
Asn Asn Ile His Ser Gly Asn Gln Ser Leu Thr Cys Leu Arg Leu Asp
115 120 125
Asn Glu Arg Ser Asn Asn Ile Gly Ile Trp Gln Lys Lys Ser Gly Ser
130 135 140
Lys Gln Ser Glu Ser Asn Trp Leu Met Lys Leu Glu Leu Asp His Asp
145 150 155 160
Gln His Gly Asn Ser Thr Leu Lys Arg Glu Thr Asp Asp Asp Ile Ala
165 170 175
Gln Met Ile Glu Glu Leu Leu Asp Cys Gly Ser Leu Glu Ile Cys Ser
180 185 190
Pro Ile Ala Ser Ala Asp Ser Asn Ile Asn Ser Ala Glu Ser Met Leu
195 200 205
Asn
<210> 325
<211> 224
<212> PRT
<213> 江南卷柏()
<220>
<223> 转录因子shine (SmSHN1), Sm92334
<400> 325
Met Gly Arg Pro Gln Arg Tyr Arg Gly Val Arg Gln Arg His Trp Gly
1 5 10 15
Ser Trp Val Ser Glu Ile Arg His Pro Leu Leu Lys Thr Arg Val Trp
20 25 30
Leu Gly Thr Phe Glu Thr Ala Glu Asp Ala Ala Arg Ala Tyr Asp Glu
35 40 45
Ala Ala Arg Leu Met Gly Gly Pro Arg Ala Arg Thr Asn Phe Pro Tyr
50 55 60
Asp Pro Asn Ala Pro Pro His Pro Ser Ser Ser Thr Leu Leu Ser Thr
65 70 75 80
Leu Ser Ala Lys Leu Asn Arg Cys Phe Ser Ser Ser Ser Ser Ser Ser
85 90 95
Ser Cys Ser Thr Asp Pro His Lys Lys Asp Pro Arg Val Ser Gln Ser
100 105 110
Leu Thr Cys Leu Arg Leu Asp Pro Glu Gln Ser Asn Leu Gly Ile Trp
115 120 125
Gln Lys Lys Ser Gly Arg Gln Pro Glu Ser Asn Trp Val Met Lys Val
130 135 140
His Phe Gly Ser Gln Gly Gly Gly Gly Val Ser Ser Asp Ile Val Leu
145 150 155 160
Pro Thr Asp Asn Pro Ala Pro Pro Gln Pro Ile Glu His Lys Lys Met
165 170 175
Lys Ser Glu Glu Asp Leu Ala Thr Glu Met Ile Glu Glu Leu Leu Asn
180 185 190
Phe Pro Asp Ser Ser Ser Pro Ser Ser Ser Thr Ser Ser Ser Glu Ala
195 200 205
Lys Asn Pro Asn Phe Ser Ser Ser Asp Leu Leu His His Ile Leu Val
210 215 220
<210> 326
<211> 212
<212> PRT
<213> 小立碗藓()
<220>
<223> 转录因子shine (PpSHN1)
<400> 326
Met Gly Arg Pro Gln Arg Tyr Arg Gly Val Arg Gln Arg His Trp Gly
1 5 10 15
Ser Trp Val Ser Glu Ile Arg His Pro Leu Leu Lys Thr Arg Val Trp
20 25 30
Leu Gly Thr Phe Glu Thr Ala Glu Asp Ala Ala His Ala Tyr Asp Glu
35 40 45
Ala Ala Arg Leu Met Cys Gly Val Arg Ala Arg Thr Asn Phe Pro Tyr
50 55 60
Asp Pro Asn Ala Ser Lys Arg Pro Asn Ser Gln Met Leu Ser Ala Thr
65 70 75 80
Leu Ser Ala Lys Leu His Arg Trp Tyr Leu His Ser Gln Gln Arg Asp
85 90 95
Gly Gln Glu Gly Lys Ser Lys Asp Ala Arg Met Thr Gln Ser Leu Thr
100 105 110
Cys Leu Cys Leu Asp Ala Glu Gln Ser Asn Leu Gly Ile Trp Gln Lys
115 120 125
Lys Thr Gly Arg Gln Ala Glu Ala Asn Trp Val Arg Lys Val Gln Phe
130 135 140
Gly Asp Asn Asn Ser Pro Gln Thr Asp Ser Pro Gln Pro Glu Asn Ser
145 150 155 160
Ser Glu Ser Cys Met Ser Glu Glu Asp Lys Phe Ala Ala Glu Met Ile
165 170 175
Glu Glu Leu Leu Gly Tyr Ser Pro Gly Gln Phe Ser Asn Phe Gly Ser
180 185 190
Pro Ala Met Ser Asp Ser Ser Cys Ser Ser Ser Cys Ser Ala Val Thr
195 200 205
Thr Ala Phe Glu
210
<210> 327
<211> 291
<212> PRT
<213> 人工序列()
<220>
<223> 合成的MYB结构域蛋白96 (MYB96) 共有 序列（大多数）
<220>
<221> 变体
<222> (120)..(.120)
<223> Xaa = Asn, Gln, Pro, Ser, Thr或Gly
<220>
<221> 变体
<222> (122)..(.122)
<223> Xaa = Ser, Phe, Thr, Gly, Ala或Arg
<220>
<221> 变体
<222> (127)..(.127)
<223> Xaa = Asn, Asp, Glu, Gly, Thr或Ala
<220>
<221> 变体
<222> (131)..(.131)
<223> Xaa = Ser, Asn, Arg, Phe, Leu, Ala, Glu, Lys或Thr
<220>
<221> 变体
<222> (133)..(.133)
<223> Xaa = His, Ser, Lys或Gln
<220>
<221> 变体
<222> (135)..(.135)
<223> Xaa = Ser, Leu, Pro, Gln, Arg, Asp或Lys
<220>
<221> 变体
<222> (153)..(.153)
<223> Xaa = Lys或Arg
<220>
<221> 变体
<222> (166)..(.166)
<223> Xaa = Ile, Pro, Gly, Ser, Ala或Asn
<220>
<221> 变体
<222> (167)..(.167)
<223> Xaa = Arg, Pro, Thr, Ser, Lys或Gly
<220>
<221> 变体
<222> (179)..(.179)
<223> Xaa = Thr, Ser, Asn, Tyr, Ala或Pro
<220>
<221> 变体
<222> (211)..(.211)
<223> Xaa = Ser, Glu, Met, Gln, Gly, Asp, Lys或Thr
<220>
<221> 变体
<222> (212)..(.212)
<223> Xaa = Pro, Met, Asn, Val, Ser, Gly或Thr
<220>
<221> 变体
<222> (219)..(.219)
<223> Xaa = Ala, Pro, Thr, Leu或Ser
<220>
<221> 变体
<222> (234)..(.234)
<223> Xaa = Ser, Arg或Gly
<220>
<221> 变体
<222> (236)..(.236)
<223> Xaa = Asn, Arg, Asp, Glu或Ala
<220>
<221> 变体
<222> (254)..(.254)
<223> Xaa = Pro, Cys, Lys, Glu或Gly
<220>
<221> 变体
<222> (255)..(.255)
<223> Xaa = Asp, Ile, Lys, Val, Ala或Gly
<220>
<221> 变体
<222> (259)..(.259)
<223> Xaa = Ile, Glu, Ser, Pro, Ala或Thr
<220>
<221> 变体
<222> (260)..(.260)
<223> Xaa = Ser, Thr, Ala或Pro
<220>
<221> 变体
<222> (266)..(.266)
<223> Xaa = Phe, Leu, Met,或Ile
<220>
<221> 变体
<222> (279)..(.279)
<223> Xaa = Met, Glu, Asp, Tyr, Gly, Pro, His或Ser
<220>
<221> 变体
<222> (283)..(.283)
<223> Xaa = Met, Val, Leu或Gly
<220>
<221> 变体
<222> (284)..(.284)
<223> Xaa = Ala, Ser, Thr, Lys, Pro, Gln或Leu
<220>
<221> UNSURE
<222> (120)..(120)
<223> The 'Xaa' at location 120 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (122)..(122)
<223> The 'Xaa' at location 122 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (127)..(127)
<223> The 'Xaa' at location 127 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (131)..(131)
<223> The 'Xaa' at location 131 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (133)..(133)
<223> The 'Xaa' at location 133 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (135)..(135)
<223> The 'Xaa' at location 135 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (153)..(153)
<223> The 'Xaa' at location 153 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (166)..(166)
<223> The 'Xaa' at location 166 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (167)..(167)
<223> The 'Xaa' at location 167 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (179)..(179)
<223> The 'Xaa' at location 179 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (211)..(211)
<223> The 'Xaa' at location 211 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (212)..(212)
<223> The 'Xaa' at location 212 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (219)..(219)
<223> The 'Xaa' at location 219 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (234)..(234)
<223> The 'Xaa' at location 234 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (236)..(236)
<223> The 'Xaa' at location 236 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (254)..(254)
<223> The 'Xaa' at location 254 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (255)..(255)
<223> The 'Xaa' at location 255 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (259)..(259)
<223> The 'Xaa' at location 259 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (260)..(260)
<223> The 'Xaa' at location 260 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (266)..(266)
<223> The 'Xaa' at location 266 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (279)..(279)
<223> The 'Xaa' at location 279 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (283)..(283)
<223> The 'Xaa' at location 283 stands for Gln, Arg, Pro, or Leu.
<220>
<221> UNSURE
<222> (284)..(284)
<223> The 'Xaa' at location 284 stands for Gln, Arg, Pro, or Leu.
<400> 327
Met Gly Arg Pro Pro Cys Cys Asp Lys Ile Gly Val Lys Lys Gly Pro
1 5 10 15
Trp Thr Pro Glu Glu Asp Ile Ile Leu Val Ser Tyr Ile Gln Glu His
20 25 30
Gly Pro Gly Asn Trp Arg Ala Val Pro Thr Asn Thr Gly Leu Met Arg
35 40 45
Cys Ser Lys Ser Cys Arg Leu Arg Trp Thr Asn Tyr Leu Arg Pro Gly
50 55 60
Ile Lys Arg Gly Asn Phe Thr Asp Gln Glu Glu Lys Met Ile Val His
65 70 75 80
Leu Gln Ala Leu Leu Gly Asn Arg Trp Ala Ala Ile Ala Ser Tyr Leu
85 90 95
Pro Glu Arg Thr Asp Asn Asp Ile Lys Asn Tyr Trp Asn Thr His Leu
100 105 110
Lys Lys Lys Leu Lys Lys Met Xaa Ala Xaa Gly Asp Glu Asp Xaa Ser
115 120 125
Ala Gly Xaa Ser Xaa Gln Xaa Ala Asn Lys Gly Gln Trp Glu Arg Arg
130 135 140
Leu Gln Thr Asp Ile His Met Ala Xaa Gln Ala Leu Cys Glu Ala Leu
145 150 155 160
Ser Leu Asp Lys Pro Xaa Xaa Pro Leu Ser Lys Pro Ser Ser Ser Ser
165 170 175
Ser Ser Xaa Thr Thr Pro Ala Thr Tyr Ala Ser Ser Ala Glu Asn Ile
180 185 190
Ala Arg Leu Leu Glu Gly Trp Met Arg Pro Gly Pro Lys Ser Asn Ser
195 200 205
Ser Thr Xaa Xaa Gly Ser Gly Ser Ser Ser Xaa Ala Ala Ser Glu Gly
210 215 220
Gly Ser Gly Ala Ser Ala Ser Leu Phe Xaa Phe Xaa Ser Ser Gln Ala
225 230 235 240
Leu Thr Pro Glu Thr Ser Thr Phe Thr Asp Lys Glu Ser Xaa Xaa Pro
245 250 255
Gly Pro Xaa Xaa Gln Pro Leu Ser Leu Xaa Glu Lys Trp Leu Phe Asp
260 265 270
Asp Gly Gln Gly His Glu Xaa Leu Ile Asp Xaa Xaa Leu Glu Asp Pro
275 280 285
Ser Leu Phe
290
<210> 328
<211> 35
<212> DNA
<213> 人工序列()
<220>
<223> 合成的扩增引物pVND6-F3-KpnI
<400> 328
cccgggtacc tcctttacga tgttgttatg ggtta 35
<210> 329
<211> 46
<212> DNA
<213> 人工序列()
<220>
<223> 合成的扩增引物pVND6-R3-SpeI
<400> 329
cccgactagt gtgtgcgaga ctttggattt gatcttttta atttta 46
<210> 330
<211> 51
<212> DNA
<213> 人工序列()
<220>
<223> 合成的扩增引物FY100908-C4h-GW-F
<400> 330
ggggacaagt ttgtacaaaa aagcaggctt catggacctc ctcttgctgg a 51
<210> 331
<211> 52
<212> DNA
<213> 人工序列()
<220>
<223> 合成的扩增引物FY100908-C4h-GW-R
<400> 331
ggggaccact ttgtacaaga aagctgggtc acagttcctt ggtttcataa cg 52
<210> 332
<211> 55
<212> DNA
<213> 人工序列()
<220>
<223> 合成的扩增引物DL-F5G3-At3g19450-GW
<400> 332
ggggacaagt ttgtacaaaa aagcaggctt catgggaagt gtagaagcag gagaa 55
<210> 333
<211> 54
<212> DNA
<213> 人工序列()
<220>
<223> 合成的扩增引物DL-R5G3-At3g19450-GW
<400> 333
ggggaccact ttgtacaaga aagctgggtc gtttgtagtt gttgcagcct cctc 54
<210> 334
<211> 52
<212> DNA
<213> 人工序列()
<220>
<223> 合成的扩增引物FY081508-F5h-1-GW-F
<400> 334
ggggacaagt ttgtacaaaa aagcaggctt catggagtct tctatatcac aa 52
<210> 335
<211> 51
<212> DNA
<213> 人工序列()
<220>
<223> 合成的扩增引物FY081508-F5h-1-GW-R
<400> 335
ggggaccact ttgtacaaga aagctgggtc aagagcacag atgaggcgcg t 51
<210> 336
<211> 20
<212> DNA
<213> 人工序列()
<220>
<223> 合成的扩增引物ref3-2F1
<400> 336
ttccgtatca tgttcgatag 20
<210> 337
<211> 20
<212> DNA
<213> 人工序列()
<220>
<223> 合成的扩增引物ref3-2R1
<400> 337
aatgtcaatt tcccaaaatc 20
<210> 338
<211> 20
<212> DNA
<213> 人工序列()
<220>
<223> 合成的扩增引物pcr-pVND6F1
<400> 338
caaattgcca cattgcagaa 20
<210> 339
<211> 21
<212> DNA
<213> 人工序列()
<220>
<223> 合成的扩增引物pcr-REF3-R1
<400> 339
cgacgagatt acggtggttg a 21

Claims (12)

1.一种工程改造植物以增加生物合成产物在期望的组织中的生产量的方法，所述方法包括：
将表达盒引入所述植物中，其中所述表达盒包含与异源启动子可操作地连接的编码转录因子的多核苷酸，所述转录因子调节所述生物合成产物的生产量，其中所述异源启动子是诱导基因的基因表达的启动子，所述基因是所述转录因子在所述期望的组织中的下游靶标；和
在表达所述转录因子的条件下，培养所述植物。
2.一种工程改造植物的方法，所述植物具有基本上集中在所述植物木质部组织导管的木质素沉积，所述方法包括：
将表达盒引入所述植物中，其中所述植物被修饰成具有降低的木质素生物合成酶表达水平；且进一步，其中所述表达盒包含与异源的导管特异性的启动子可操作地连接的编码所述木质素生物合成酶的多核苷酸；和
在表达所述木质素生物合成酶的条件下，培养所述植物。
3.一种在糖化反应中获得增加的量的来自植物的可溶性糖的方法，所述方法包括：
使植物进行糖化反应，由此与野生型植物相比，增加能够从所述植物得到的可溶性糖的量,
其中所述植物是通过根据权利要求1或2所述的方法工程改造的植物或其后代；或
其中所述植物包含与异源的导管特异性的启动子可操作地连接的编码木质素生物合成酶的多核苷酸，并且具有基本上集中在所述植物木质部组织导管的木质素沉积。
4.一种工程改造植物的方法，所述植物具有增加的次生细胞壁沉积，所述方法包括：
将表达盒引入所述植物中，其中所述表达盒包含与异源启动子可操作地连接的编码转录因子的多核苷酸，所述转录因子调节次生细胞壁在木质组织中的生产量，其中所述启动子增强作为所述转录因子的下游靶标的基因的表达；和
在表达所述转录因子的条件下，培养所述植物。
5.一种增加源自植物的生物质的生物能生产量的方法，所述方法包括：
从植物收获生物质；和
使所述生物质进行转化反应，由此与野生型植物相比增加生物能生产量；
其中所述植物是通过根据权利要求4所述的方法工程改造的植物或其后代；或
其中所述植物具有增加的次生壁沉积，并且包含表达盒，所述表达盒包含与异源启动子可操作地连接的编码转录因子的多核苷酸，所述转录因子调节次生细胞壁在木质组织中的生产量，其中所述启动子是诱导作为所述转录因子的下游靶标的基因的表达的启动子；或
其中所述植物具有基本上集中在所述植物木质部组织导管的木质素沉积，并且包含表达盒，所述表达盒包含与异源启动子可操作地连接的编码转录因子的多核苷酸，所述转录因子调节次生细胞壁在木质组织中的生产量，其中所述启动子是诱导作为所述转录因子的下游靶标的基因的表达的启动子。
6.一种在生长过程中增加植物的茎、秆或木料强度的方法，所述方法包括
培养植物以产生种子，其中抗倒伏性与野生型植物相比得到提高；
其中所述植物是通过根据权利要求4所述的方法工程改造的植物或其后代；或
其中所述植物具有增加的次生壁沉积，并且包含表达盒，所述表达盒包含与异源启动子可操作地连接的编码转录因子的多核苷酸，所述转录因子调节次生细胞壁在木质组织中的生产量，其中所述启动子是诱导作为所述转录因子的下游靶标的基因的表达的启动子。
7.一种获得具有提高的机械应力抵抗力的植物的方法，所述方法包括：
培养植物，由此获得与野生型植物相比具有提高的机械应力抵抗力的植物，所述野生型植物没有经过遗传修饰来增加次生细胞壁产生；
其中所述植物是通过根据权利要求4所述的方法工程改造的植物或其后代；或
其中所述植物具有增加的次生壁沉积，并且包含表达盒，所述表达盒包含与异源启动子可操作地连接的编码转录因子的多核苷酸，所述转录因子调节次生细胞壁在木质组织中的生产量，其中所述启动子是诱导作为所述转录因子的下游靶标的基因的表达的启动子。
8.一种工程改造植物的方法，所述植物具有基本上集中在所述植物木质部组织导管的木聚糖沉积，所述方法包括：
将表达盒引入所述植物中，其中所述植物被修饰成具有降低的木聚糖生物合成酶表达水平；且进一步，其中所述表达盒包含与异源的导管特异性的启动子可操作地连接的编码所述木聚糖生物合成酶的多核苷酸；和
在表达所述木聚糖生物合成酶的条件下，培养所述植物。
9.一种在糖化反应中获得增加的量的来自植物的可溶性糖的方法，所述方法包括：
使植物进行糖化反应，由此与野生型植物相比，增加能够从所述植物得到的可溶性糖的量；
其中所述植物是通过根据权利要求8所述的方法工程改造的植物或其后代；或
其中所述植物具有基本上集中在所述植物木质部组织导管的木聚糖沉积，并且包含表达盒，所述表达盒包含与异源的导管特异性的启动子可操作地连接的编码木聚糖生物合成酶的多核苷酸。
10.一种在水解反应后获得增加的来自植物的C6/C5糖比率的方法，所述方法包括：
使植物进行化学水解或酶促水解，由此与野生型植物相比增加能够从所述植物得到的C6糖相对于C5糖的量；
其中所述植物是通过根据权利要求8所述的方法工程改造的植物或其后代；或
其中所述植物具有基本上集中在所述植物木质部组织导管的木聚糖沉积，并且包含表达盒，所述表达盒包含与异源的导管特异性的启动子可操作地连接的编码木聚糖生物合成酶的多核苷酸。
11.一种工程改造植物的方法，所述植物具有基本上集中在所述植物木质部组织导管的木聚糖O-乙酰化，所述方法包括：
将表达盒引入所述植物中，其中所述植物被修饰成具有降低的负责木聚糖O-乙酰化的酶的表达水平；且进一步，其中所述表达盒包含与异源的导管特异性的启动子可操作地连接的编码所述木聚糖O-乙酰化酶的多核苷酸；和
在表达所述木聚糖O-乙酰化酶的条件下，培养所述植物。
12.一种在糖化反应中获得增加的量的来自植物的可溶性糖的方法，所述方法包括：
使植物进行糖化反应，由此与野生型植物相比，增加能够从所述植物得到的可溶性糖的量；
其中所述植物是通过根据权利要求11所述的方法工程改造的植物或其后代；或
其中所述植物具有基本上集中在所述植物木质部组织导管的木聚糖O-乙酰化，并且包含表达盒，所述表达盒包含与异源的导管特异性的启动子可操作地连接的编码所述木聚糖O-乙酰化酶的多核苷酸。
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CN109576284B (zh) * 2018-12-21 2021-09-17 中国农业科学院北京畜牧兽医研究所 一个多功能的myb转录因子基因及其用途
CN110699368A (zh) * 2019-11-21 2020-01-17 安徽农业大学 小麦肉桂酸-4-羟基化酶基因及其扩增引物和应用
CN111410684A (zh) * 2020-05-12 2020-07-14 中国农业科学院作物科学研究所 SiMYB30蛋白质及其相关生物材料在调控植物耐逆性和产量中的应用
CN111499713A (zh) * 2020-06-10 2020-08-07 华中农业大学 水稻粒型基因qGL6-2及其应用
CN113234734B (zh) * 2021-03-22 2022-10-25 成都大学 一种可提高植物抗性的甜橙基因CsMYB30及其应用
CN113234734A (zh) * 2021-03-22 2021-08-10 成都大学 一种可提高植物抗性的甜橙基因CsMYB30及其应用
CN113403322A (zh) * 2021-05-14 2021-09-17 云南大学 一种茶树干旱响应基因CsNAC168及其编码蛋白和应用
CN115820687A (zh) * 2022-10-14 2023-03-21 海南大学 柚子中绿原酸生物合成途径基因CgHCT和CgC3′H及其应用
CN115820687B (zh) * 2022-10-14 2024-03-19 海南大学 柚子中绿原酸生物合成途径基因CgHCT和CgC3′H及其应用
CN116987710A (zh) * 2023-08-07 2023-11-03 西部(重庆)科学城种质创制大科学中心 马铃薯耐旱性相关基因StMYB55及其应用
CN116987710B (zh) * 2023-08-07 2024-05-28 西部(重庆)科学城种质创制大科学中心 马铃薯耐旱性相关基因StMYB55及其应用

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CN107674882B (zh) 2021-06-25
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US20140298539A1 (en) 2014-10-02
CN103403016A (zh) 2013-11-20
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