CN107580891A - A kind of cuttage seedling raising method for improving Chinese tallow tree cold tolerance - Google Patents
A kind of cuttage seedling raising method for improving Chinese tallow tree cold tolerance Download PDFInfo
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- CN107580891A CN107580891A CN201710863171.0A CN201710863171A CN107580891A CN 107580891 A CN107580891 A CN 107580891A CN 201710863171 A CN201710863171 A CN 201710863171A CN 107580891 A CN107580891 A CN 107580891A
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Abstract
The present invention relates to a kind of cuttage seedling raising method for improving Chinese tallow tree cold tolerance, specially following steps:(1)The interception of cutting;(2)Cuttage pre-treatment;(3)Cuttage is transplanted to seedbed after the 60cm of height of seedling 50.By experiment, use a kind of cuttage seedling raising method for improving Chinese tallow tree cold tolerance provided by the invention, significantly improve the cold tolerance of Chinese tallow tree tree cuttage seedling, under cuttage transplantation of seedlings to the low temperature environment that will be obtained using the present invention, survival rate is up to more than 78% after 2 weeks, and the cuttage seeding obtained using prior art is cultivated 2 weeks at low ambient temperatures, survival rate is only 42%, it is seen that cold tolerance significant effect of the present invention for raising Chinese tallow tree.
Description
Technical field
The invention belongs to field of garden technology, and in particular to a kind of cuttage seedling raising method for improving Chinese tallow tree cold tolerance.
Background technology
Chinese tallow tree, Euphorbiaceae, sapium deciduous tree, it is a kind of Colour-leaved trees, spring and autumn leaf color is red gorgeous brilliant, special for China
Some economic trees, existing more than 1400 years cultivation history.Chinese tallow tree is stronger to the adaptability of soil, is the strong arbor tree of salt-resistance
One of kind.Chinese tallow tree has stronger resistance to poisonous hydrogen fluoride gas.
Chinese tallow tree is used as medicine with root skin, bark, leaf.Root skin and the bark four seasons can be adopted, and section is dried;The more using fresh herbs of leaf, desinsection, solution
Poison, diuresis, defaecation.It is unfavorable for snail fever, cirrhotic ascites, stool and urine, venomous snake bite;Furunculosis, corn, mammary gland are controlled in external application
Inflammation, traumatic injury, eczema, dermatitis.
The asexual reproduction method of Chinese tallow tree is usually cuttage and grafting, and wherein cuttage seedling raising method is the most commonly used.In order to
More excellent seeds are cultivated, the cuttage and seedling culture method of Chinese tallow tree is also required to constantly weed out the old and bring forth the new, and could meet the market demand.
The content of the invention
In order to solve the above problems, the invention provides a kind of cuttage seedling raising method for improving Chinese tallow tree cold tolerance.
The present invention is achieved through the following technical solutions:
A kind of cuttage seedling raising method for improving Chinese tallow tree cold tolerance, specially following steps:
(1)The interception of cutting
Select excellent maternal plant, in the time in early spring, choose suitable branch, branch block position first block three using instrument/
One, nutritive cream then is smeared in truncated position, and wound with plastic cloth, truncated position starts to bear callus after 7-10 days, will
Branch thoroughly intercepts branch along truncated position as cutting, and only retains bud-leaf concordant with top in cutting;In the time in early spring, tree
Liquid stream is dynamic slow, now intercepts 1/3rd branches, smears the nutritive cream and wrap up, can effective stimulus truncated position cell metabolism
Activity, treat that truncated position forms callus, then intercept branch as cutting, can ensure to support soil during cutting cuttage
The absorption divided, greatly improves its rooting rate, simultaneously because plant cell metabolic rate is exceedingly fast at callus, effectively carries
High reaching maturity for cutting immune system, makes it have more preferable cold tolerance;
(2)Cuttage pre-treatment
The cutting 50-80 of interception is tied for a bundle, end is placed in immersion 30-40 minutes in auxin solution, every during immersion
Applied the 0.2A alternating current 30-40 seconds into auxin solution every 5 minutes, then use and cutting is placed in the nutrient solution of normal temperature
Water planting, oxygenerator oxygenation into the nutrient solution is needed to use during water planting, after adventitious root is born in cutting, by the nutrition
Liquid temperature degree is reduced to 10-15 DEG C, continues water planting until cutting coring average length is 2-4cm;The nutrient solution, its preparation side
Method, specially following steps:
A according to the mass fraction, takes calophyllum inophyllum 15-20 parts, saussurea involucrata 2-3 parts, Radix Astragali 20-25 parts, Ligusticum wallichii 8-10 parts, wilsonii 5-11
Part dries after cleaning, and is crushed to 40 mesh, and ethanol solution 200-220 parts that then addition mass fraction is 70% are in 75-85 DEG C of water-bath
20-30 minutes are heated, are filtered to get filtrate, the dregs of a decoction, the ethanol reclaimed in the filtrate obtains extract solution;
The Angel Yeast bacterium bacterium solution that B adds the dregs of a decoction 1.5 times of its mass fractions is sealed by fermentation 180-200 minutes, then
1000MHz microwave heat sterilizations, add 5 times of dilutions of water, filtering and impurity removing, obtain secondary raffinate;
C merges the extract solution and secondary raffinate, plant extraction liquid is obtained, then by the plant extraction liquid and taurine, Asia
Sodium selenate, iron chloride, calcium dihydrogen phosphate, potassium sulfate, urea 100-120 in mass ratio:0.1:0.3:0.1:0.8:1:2 is uniformly mixed
The nutrient solution is produced after conjunction;
By cutting using alternating current is applied in auxin solution immersion process, effective stimulus cutting cell of termination is to auxin solution
Absorption, stimulate the growth of root, then using the suspension culture, ensure the various nutrients described in cutting, while oxygenerator
The oxygen of abundance is ensure that, contributes to further reaching maturity for cutting plant immune system, adventitious root to be born, described in reduction
Solution temperature, there is good stress exercise effect to cutting, further improve the cold tolerance of cutting;
(3)Cuttage is transplanted to seedbed after height of seedling 50-60cm.
Further, step(1)The nutritive cream, by aloe ointment, kerosene, glycerine, inositol, glucose in mass ratio
20-30:10:15:0.1:It is made after 1 mixing.
Further, step(2)The Angel Yeast bacterium bacterium solution, bacterial concentration 1250cfu/mL.
Beneficial effects of the present invention:In the time in early spring, resin flowing is slow, now intercepts 1/3rd branches, smears institute
State nutritive cream and wrap up, energy effective stimulus truncated position metabolic activity in cells, treat that truncated position forms callus, then intercept branch work
For cutting, the absorption of the nutrient during cutting cuttage to soil can be ensured, greatly improve its rooting rate, simultaneously because more
Plant cell metabolic rate is exceedingly fast at injured tissue, effectively increases reaching maturity for cutting immune system, makes it have more
Good cold tolerance;By cutting using applying alternating current, effective stimulus cutting cell of termination pair in auxin solution immersion process
The absorption of auxin solution, the growth of root is stimulated, then using the suspension culture, ensures the various nutrients described in cutting,
Oxygenerator ensure that the oxygen of abundance simultaneously, contribute to further reaching maturity for cutting plant immune system, and it is indefinite to wait to bear
Root, the solution temperature is reduced, there is good stress exercise effect to cutting, further improve the cold tolerance of cutting.Through
Experiment is crossed, using a kind of cuttage seedling raising method for improving Chinese tallow tree cold tolerance provided by the invention, significantly improves Chinese tallow tree skewer
The cold tolerance of seedlings plugging, will using under cuttage transplantation of seedlings to the low temperature environment that obtains of the present invention, after 2 weeks survival rate be up to 78% with
On, and the cuttage seeding obtained using prior art is cultivated 2 weeks at low ambient temperatures, and survival rate is only 42%, it is seen that it is of the invention for
Improve the cold tolerance significant effect of Chinese tallow tree.
Embodiment
Embodiment 1
A kind of cuttage seedling raising method for improving Chinese tallow tree cold tolerance, specially following steps:
(1)The interception of cutting
Select excellent maternal plant, in the time in early spring, choose suitable branch, branch block position first block three using instrument/
One, nutritive cream then is smeared in truncated position, and wound with plastic cloth, truncated position starts to bear callus after 7-10 days, will
Branch thoroughly intercepts branch along truncated position as cutting, and only retains bud-leaf concordant with top in cutting;
(2)Cuttage pre-treatment
The cutting 50-80 of interception is tied for a bundle, end, which is placed in auxin solution, soaks 30 minutes, every 5 during immersion
Minute alternating current from application 0.2A to auxin solution 30 seconds, then uses and cutting is placed in water planting in the nutrient solution of normal temperature,
Oxygenerator oxygenation into the nutrient solution is needed to use during water planting, after adventitious root is born in cutting, by the nutrition liquid temperature
Degree is reduced to 10-15 DEG C, continues water planting until cutting coring average length is 2-4cm;The nutrient solution, its preparation method, tool
Body is following steps:
A according to the mass fraction, takes 15 parts of calophyllum inophyllum, 2 parts of saussurea involucrata, 20 parts of the Radix Astragali, 8 parts of Ligusticum wallichii, 5 parts of wilsonii to be dried after cleaning,
40 mesh are crushed to, 200 parts of ethanol solution that mass fraction is 70% is then added in 75-85 DEG C of heating water bath 20 minutes, filters
Filtrate, the dregs of a decoction, the ethanol reclaimed in the filtrate obtain extract solution;
The Angel Yeast bacterium bacterium solution that B adds the dregs of a decoction 1.5 times of its mass fractions is sealed by fermentation 180 minutes, then 1000MHz
Microwave heat sterilization, add 5 times of dilutions of water, filtering and impurity removing, obtain secondary raffinate;
C merges the extract solution and secondary raffinate, plant extraction liquid is obtained, then by the plant extraction liquid and taurine, Asia
Sodium selenate, iron chloride, calcium dihydrogen phosphate, potassium sulfate, urea in mass ratio 100:0.1:0.3:0.1:0.8:1:After 2 uniformly mix
Produce the nutrient solution;
(3)Cuttage is transplanted to seedbed after height of seedling 50-60cm.
Further, step(1)The nutritive cream, by aloe ointment, kerosene, glycerine, inositol, glucose in mass ratio
20-30:10:15:0.1:It is made after 1 mixing.
Further, step(2)The Angel Yeast bacterium bacterium solution, bacterial concentration 1250cfu/mL.
Embodiment 2
A kind of cuttage seedling raising method for improving Chinese tallow tree cold tolerance, specially following steps:
(1)The interception of cutting
Select excellent maternal plant, in the time in early spring, choose suitable branch, branch block position first block three using instrument/
One, nutritive cream then is smeared in truncated position, and wound with plastic cloth, truncated position starts to bear callus after 7-10 days, will
Branch thoroughly intercepts branch along truncated position as cutting, and only retains bud-leaf concordant with top in cutting;
(2)Cuttage pre-treatment
The cutting 50-80 of interception is tied for a bundle, end, which is placed in auxin solution, soaks 40 minutes, every 5 during immersion
Minute alternating current from application 0.2A to auxin solution 40 seconds, then uses and cutting is placed in water planting in the nutrient solution of normal temperature,
Oxygenerator oxygenation into the nutrient solution is needed to use during water planting, after adventitious root is born in cutting, by the nutrition liquid temperature
Degree is reduced to 10-15 DEG C, continues water planting until cutting coring average length is 2-4cm;The nutrient solution, its preparation method, tool
Body is following steps:
A according to the mass fraction, takes 20 parts of calophyllum inophyllum, 3 parts of saussurea involucrata, 25 parts of the Radix Astragali, 10 parts of Ligusticum wallichii, 11 parts of wilsonii to be dried after cleaning
It is dry, 40 mesh are crushed to, then add 220 parts of ethanol solution that mass fraction is 70% in 75-85 DEG C of heating water bath 30 minutes, mistake
Filtrate, the dregs of a decoction are filtered to obtain, the ethanol reclaimed in the filtrate obtains extract solution;
The Angel Yeast bacterium bacterium solution that B adds the dregs of a decoction 1.5 times of its mass fractions is sealed by fermentation 200 minutes, then 1000MHz
Microwave heat sterilization, add 5 times of dilutions of water, filtering and impurity removing, obtain secondary raffinate;
C merges the extract solution and secondary raffinate, plant extraction liquid is obtained, then by the plant extraction liquid and taurine, Asia
Sodium selenate, iron chloride, calcium dihydrogen phosphate, potassium sulfate, urea in mass ratio 120:0.1:0.3:0.1:0.8:1:After 2 uniformly mix
Produce the nutrient solution;
(3)Cuttage is transplanted to seedbed after height of seedling 50-60cm.
Further, step(1)The nutritive cream, by aloe ointment, kerosene, glycerine, inositol, glucose in mass ratio
20-30:10:15:0.1:It is made after 1 mixing.
Further, step(2)The Angel Yeast bacterium bacterium solution, bacterial concentration 1250cfu/mL.
Embodiment 3
A kind of cuttage seedling raising method for improving Chinese tallow tree cold tolerance, specially following steps:
(1)The interception of cutting
Select excellent maternal plant, in the time in early spring, choose suitable branch, branch block position first block three using instrument/
One, nutritive cream then is smeared in truncated position, and wound with plastic cloth, truncated position starts to bear callus after 7-10 days, will
Branch thoroughly intercepts branch along truncated position as cutting, and only retains bud-leaf concordant with top in cutting;
(2)Cuttage pre-treatment
The cutting 50-80 of interception is tied for a bundle, end, which is placed in auxin solution, soaks 35 minutes, every 5 during immersion
Minute alternating current from application 0.2A to auxin solution 35 seconds, then uses and cutting is placed in water planting in the nutrient solution of normal temperature,
Oxygenerator oxygenation into the nutrient solution is needed to use during water planting, after adventitious root is born in cutting, by the nutrition liquid temperature
Degree is reduced to 10-15 DEG C, continues water planting until cutting coring average length is 2-4cm;The nutrient solution, its preparation method, tool
Body is following steps:
A according to the mass fraction, takes 18 parts of calophyllum inophyllum, 2.5 parts of saussurea involucrata, 22 parts of the Radix Astragali, 9 parts of Ligusticum wallichii, 8 parts of wilsonii to be dried after cleaning
It is dry, 40 mesh are crushed to, then add 210 parts of ethanol solution that mass fraction is 70% in 75-85 DEG C of heating water bath 25 minutes, mistake
Filtrate, the dregs of a decoction are filtered to obtain, the ethanol reclaimed in the filtrate obtains extract solution;
The Angel Yeast bacterium bacterium solution that B adds the dregs of a decoction 1.5 times of its mass fractions is sealed by fermentation 190 minutes, then 1000MHz
Microwave heat sterilization, add 5 times of dilutions of water, filtering and impurity removing, obtain secondary raffinate;
C merges the extract solution and secondary raffinate, plant extraction liquid is obtained, then by the plant extraction liquid and taurine, Asia
Sodium selenate, iron chloride, calcium dihydrogen phosphate, potassium sulfate, urea in mass ratio 110:0.1:0.3:0.1:0.8:1:After 2 uniformly mix
Produce the nutrient solution;
(3)Cuttage is transplanted to seedbed after height of seedling 50-60cm.
Further, step(1)The nutritive cream, by aloe ointment, kerosene, glycerine, inositol, glucose in mass ratio
20-30:10:15:0.1:It is made after 1 mixing.
Further, step(2)The Angel Yeast bacterium bacterium solution, bacterial concentration 1250cfu/mL.
Comparative example 1
This comparative example 1 compared with Example 1, without step(1)The operation, cutting is intercepted using only conventional method,
Method and step all same in addition.
Comparative example 2
This comparative example 2 compared with Example 2, without step(2)The operation, using only conventional method, in addition
Method and step all same.
Control group
Existing Chinese tallow tree cuttage and seedling culture method.
By above-mentioned 6 kinds of methods obtain the age of tree is identical, growing way identical Chinese tallow tree tree cuttage seedling is transplanted into 0 DEG C of environment, move
The survival rate of cuttage seeding, experimental result such as table 1 are observed after planting two weeks:
Table 1
Survival rate(%) | |
Embodiment 1 | 78 |
Embodiment 2 | 80 |
Embodiment 3 | 81 |
Comparative example 1 | 59 |
Comparative example 2 | 53 |
Control group | 42 |
As shown in Table 1, using a kind of cuttage seedling raising method for improving Chinese tallow tree cold tolerance provided by the invention, crow is significantly improved
The cold tolerance of Chinese tallow tree tree cuttage seedling, will be using under cuttage transplantation of seedlings to the low temperature environment of the invention obtained, and survival rate is up to after 2 weeks
More than 78%, and the cuttage seeding obtained using prior art is cultivated 2 weeks at low ambient temperatures, survival rate is only 42%, it is seen that this hair
The bright cold tolerance significant effect for improving Chinese tallow tree.
Claims (4)
1. a kind of cuttage seedling raising method for improving Chinese tallow tree cold tolerance, it is characterised in that be specially following steps:
(1)The interception of cutting
Select excellent maternal plant, in the time in early spring, choose suitable branch, branch block position first block three using instrument/
One, nutritive cream then is smeared in truncated position, and wound with plastic cloth, truncated position starts to bear callus after 7-10 days, will
Branch thoroughly intercepts branch along truncated position as cutting, and only retains bud-leaf concordant with top in cutting;
(2)Cuttage pre-treatment
The cutting 50-80 branch of interception is tied for a bundle, end is placed in immersion 30-40 minutes in auxin solution, during immersion
Apply the 0.2A alternating current 30-40 seconds into auxin solution every 5 minutes, then use the nutrient solution that cutting is placed in normal temperature
Middle water planting, oxygenerator oxygenation into the nutrient solution is needed to use during water planting, after adventitious root is born in cutting, by the battalion
Nutrient solution temperature is reduced to 10-15 DEG C, continues water planting until cutting coring average length is 2-4cm;The nutrient solution, its preparation side
Method, specially following steps:
A according to the mass fraction, takes calophyllum inophyllum 15-20 parts, saussurea involucrata 2-3 parts, Radix Astragali 20-25 parts, Ligusticum wallichii 8-10 parts, wilsonii 5-11
Part dries after cleaning, and is crushed to 40 mesh, and ethanol solution 200-220 parts that then addition mass fraction is 70% are in 75-85 DEG C of water-bath
20-30 minutes are heated, are filtered to get filtrate, the dregs of a decoction, the ethanol reclaimed in the filtrate obtains extract solution;
The Angel Yeast bacterium bacterium solution that B adds the dregs of a decoction 1.5 times of its mass fractions is sealed by fermentation 180-200 minutes, then
1000MHz microwave heat sterilizations, add 5 times of dilutions of water, filtering and impurity removing, obtain secondary raffinate;
C merges the extract solution and secondary raffinate, plant extraction liquid is obtained, then by the plant extraction liquid and taurine, Asia
Sodium selenate, iron chloride, calcium dihydrogen phosphate, potassium sulfate, urea 100-120 in mass ratio:0.1:0.3:0.1:0.8:1:2 is uniformly mixed
The nutrient solution is produced after conjunction;
(3)Cuttage is transplanted to seedbed after height of seedling 50-60cm.
A kind of 2. cuttage seedling raising method for improving Chinese tallow tree cold tolerance according to claim 1, it is characterised in that:Step
(1)The nutritive cream, by aloe ointment, kerosene, glycerine, inositol, glucose 20-30 in mass ratio:10:15:0.1:After 1 mixing
It is made.
A kind of 3. cuttage seedling raising method for improving Chinese tallow tree cold tolerance according to claim 1, it is characterised in that step
(2)The nutrient solution, specially its preparation method, following steps:
A according to the mass fraction, takes 18 parts of calophyllum inophyllum, 2.5 parts of saussurea involucrata, 22 parts of the Radix Astragali, 9 parts of Ligusticum wallichii, 8 parts of wilsonii to be dried after cleaning
It is dry, 40 mesh are crushed to, then add 210 parts of ethanol solution that mass fraction is 70% in 75-85 DEG C of heating water bath 25 minutes, mistake
Filtrate, the dregs of a decoction are filtered to obtain, the ethanol reclaimed in the filtrate obtains extract solution;
The Angel Yeast bacterium bacterium solution that B adds the dregs of a decoction 1.5 times of its mass fractions is sealed by fermentation 190 minutes, then 1000MHz
Microwave heat sterilization, add 5 times of dilutions of water, filtering and impurity removing, obtain secondary raffinate;
C merges the extract solution and secondary raffinate, plant extraction liquid is obtained, then by the plant extraction liquid and taurine, Asia
Sodium selenate, iron chloride, calcium dihydrogen phosphate, potassium sulfate, urea in mass ratio 110:0.1:0.3:0.1:0.8:1:After 2 uniformly mix
Produce the nutrient solution.
A kind of 4. cuttage seedling raising method of raising Chinese tallow tree cold tolerance according to claim 1 or 3, it is characterised in that:Institute
State Angel Yeast bacterium bacterium solution, bacterial concentration 1250cfu/mL.
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Cited By (2)
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CN108834590A (en) * | 2018-06-08 | 2018-11-20 | 阜南县宏达工艺品厂 | A kind of high viability purple willow cuttage and seedling culture method |
CN110558069A (en) * | 2019-09-29 | 2019-12-13 | 贵州黔西南喀斯特区域发展研究院 | method for improving survival rate of cutting seedling of thick-shell erythroma fruit tea |
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