CN107556120A - A kind of bio-bacterial manure and its application - Google Patents
A kind of bio-bacterial manure and its application Download PDFInfo
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- CN107556120A CN107556120A CN201711016153.5A CN201711016153A CN107556120A CN 107556120 A CN107556120 A CN 107556120A CN 201711016153 A CN201711016153 A CN 201711016153A CN 107556120 A CN107556120 A CN 107556120A
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Abstract
The invention discloses a kind of bio-bacterial manure and its application, belongs to fertilizer preparing technical field.Including following raw material：Compound bacteria, radix scutellariae powder, capsule of weeping forsythia powder, kudzu-vine root powder, earthworm powder, polyethylene glycol oxide, polybutene acid ammonium, brassinosteroid, chitosan oligosaccharide.The bio-bacterial manure is through made of the steps such as everfermentation, concentration, crushing, granulation.The bio-bacterial manure of the present invention is applied in maize planting, corn can be promoted quickly to grow up healthy and sound, and improves corn protein, the content of vitamin.
Description
【Technical field】
The invention belongs to fertilizer preparing technical field, and in particular to a kind of bio-bacterial manure and its application.
【Background technology】
Eco-fertilizer is primarily referred to as microbial manure, and it is to cause crop to obtain specific fertilizer effect with the vital movement of microorganism
A kind of product answered, it is one kind that fertilizer is used in agricultural production.It has the history of nearly 50 years in China, from rhizobium
Agent --- bacterial fertilizer --- microbial manure, from differentiation nominally it is stated that the mistake that China's microbial manure progressively develops
Journey.But for a long time, because some bacterial manure effects are unstable, bacterium number is few, invalid and harmful miscellaneous bacteria is more, bacterium in soil into
Motility rate is low, causes bacterial manure undesirable to the effect of crop yield.
A kind of Chinese patent application document " new bacterial manure (publication number：CN104446942A a kind of novel bacteria) " is disclosed
Fertilizer, including nitrogen-fixing bacteria 60-80 parts, silicate bacteria 80-100 parts, Paenibacillus polymyxa 25-45 parts, photosynthetic bacteria 30-50 parts, ferment
Female bacterium 80-110 parts, radix scutellariae powder 15-20 parts, capsule of weeping forsythia powder 10-15 parts, kudzu-vine root powder 8-10 parts.By the above-mentioned means, the invention is made
New bacterial manure, have obvious effect to early ripening of the crops, and quick a, positive effect, there is stronger slow releasing function.But there is
Using the corn protein obtained after the new bacterial manure, the content of vitamin is relatively low the problem of.
【The content of the invention】
The present invention provides a kind of bio-bacterial manure and its application, to solve in a kind of Chinese patent application document " new bacterial manure
(publication number：CN104446942A disclosed in) " on novel bacteria fertilizer formula and method, how to optimize component, dosage, method, improve
The content of corn protein, vitamin.
To solve above technical problem, the present invention uses following technical scheme：
A kind of bio-bacterial manure, including following raw material：Compound bacteria, radix scutellariae powder, capsule of weeping forsythia powder, kudzu-vine root powder, earthworm powder, polyoxyethylene
Alkene, polybutene acid ammonium, brassinosteroid, chitosan oligosaccharide；
The compound bacteria, polyethylene glycol oxide, polybutene acid ammonium, brassinosteroid, weight ratio=(250- of chitosan oligosaccharide
380):(2-4):(1.5-3):(0.5-0.8):(0.3-0.6)；
The preparation method of described bio-bacterial manure, comprises the following steps：
S1：Compound bacteria, radix scutellariae powder, capsule of weeping forsythia powder, kudzu-vine root powder, earthworm powder, polyethylene glycol oxide are mixed in proportion, in ultrasonic wave
Fermented under effect, zymotic fluid is made；
S2：Zymotic fluid made from step S1 is stood and abandons precipitation, and remaining ferment liquid is concentrated, concentrate is obtained, by institute
Concentrate drying is stated, mixture is made；
S3：Mixture made from step S2 is crushed, after sifting out with polybutene acid ammonium, brassinosteroid, chitosan oligosaccharide by
Ratio is well mixed, and particle is made, and bio-bacterial manure is made.
Preferably, described compound bacteria is in units of parts by weight, including following raw material：Bacillus 25-40 parts, nitrobacteria
20-35 parts, saccharomycete 12-20 parts, photosynthetic bacteria 5-10 parts, rhizobium 6-12 parts, phosphobacteria 5-9 parts, B. mucilaginocus 4-8 parts.
It is highly preferred that described compound bacteria is in units of parts by weight, including following raw material：35 parts of Bacillus, nitrobacteria
28 parts, 16 parts of saccharomycete, 8 parts of photosynthetic bacteria, 9 parts of rhizobium, 8 parts of phosphobacteria, 6 parts of B. mucilaginocus.
Preferably, the condition fermented described in step S1 under ul-trasonic irradiation：It is 150-300W in ultrasonic power,
Temperature is to be fermented at 50-60 DEG C, is fermented 4-6 days.
Preferably, remaining zymotic fluid concentrates at 54-65 DEG C described in step S2.
Preferably, concentrate described in step S2 is dried at 70-76 DEG C.
Preferably, the drying is to water content≤6%.
Preferably, sieve described in step S3 is 100-200 mesh.
Preferably, the particle diameter of particle described in step S3 is 0.1-2mm.
The present invention also provides a kind of application of bio-bacterial manure, applied in maize planting.
The invention has the advantages that：
(1) from embodiment 1-3 and the data of comparative example 7, using the corn protein of embodiment 1-3 bio-bacterial manures,
The content of vitamin significantly improves, and resistance enhancing, quality improves；It is from embodiment 1-3 data, embodiment 2 simultaneously
Optimum embodiment；
(2) from embodiment 2 and comparative example 1-6 data, compound bacteria, polyethylene glycol oxide, polybutene acid ammonium, rape element
Lactone, chitosan oligosaccharide serve synergy in bio-bacterial manure is prepared, and collaboration improves the content of corn protein, vitamin,
Wherein compound bacteria plays the effect of key in the content for improving corn protein, vitamin；
(3) bio-bacterial manure using effect of the invention is good, is advantageous to popularization and application.
【Embodiment】
It is specifically described with reference to specific embodiment.
Embodiment 1
A kind of bio-bacterial manure, in units of parts by weight, including following raw material：252 parts of compound bacteria, 23 parts of radix scutellariae powder, the capsule of weeping forsythia
18 parts of powder, 12 parts of kudzu-vine root powder, 8 parts of earthworm powder, 2 parts of polyethylene glycol oxide, 1.5 parts of polybutene acid ammonium, 0.5 part of brassinosteroid, shell
0.3 part of oligosaccharides；
Described compound bacteria is in units of parts by weight, including following raw material：26 parts of Bacillus, 20 parts of nitrobacteria, yeast
12 parts of bacterium, 5 parts of photosynthetic bacteria, 6 parts of rhizobium, 5 parts of phosphobacteria, 4 parts of B. mucilaginocus；
The preparation method of described bio-bacterial manure, comprises the following steps：
S1：Compound bacteria, radix scutellariae powder, capsule of weeping forsythia powder, kudzu-vine root powder, earthworm powder, polyethylene glycol oxide are mixed in proportion, in ultrasonic wave
Power is 150W, and temperature is to be fermented at 50 DEG C, ferments 6 days, zymotic fluid is made；
The preparation method of compound bacteria, comprises the following steps described in step S1：
A, respectively by Bacillus, nitrobacteria, saccharomycete, photosynthetic bacteria, rhizobium, phosphobacteria, B. mucilaginocus in proportion in temperature
Spend to be activated at 23 DEG C, expanding culture, with standby；
B, the Bacillus after culture, nitrobacteria, saccharomycete, photosynthetic bacteria, rhizobium, phosphobacteria, potassium will be expanded to step a
Bacterium is mixed and adds biological growth stimulant, biological nutritive agent, 460 parts of water are well mixed, and 18h is cultivated at being 24 DEG C in temperature
Afterwards, water removal is centrifuged in the case where rotating speed is 2000r/min, compound bacteria is made；The biological nutritive agent is in units of parts by weight, bag
Include following raw material：52 parts of yeast extract, 45 parts of sucrose, 20 parts of peptone, 22 parts of potassium acetate, 12 parts of sodium phosphate；The biological growth
Stimulant is in units of parts by weight, including following raw material：50 parts of cerium chloride, 30 parts of aluminum sulfate；
S2：Zymotic fluid made from step S1 is stood and abandons precipitation, and remaining ferment liquid is concentrated at 54 DEG C, is concentrated
Thing, it is 6% that the concentrate is dried to water content at 70 DEG C, and mixture is made；
S3：Mixture made from step S2 is crushed, it is few with polybutene acid ammonium, brassinosteroid, shell after 100 mesh sieve excessively
Sugar mixes in proportion, and the particle that particle diameter is 0.1mm is made, bio-bacterial manure is made.
Embodiment 2
A kind of bio-bacterial manure, in units of parts by weight, including following raw material：325 parts of compound bacteria, 28 parts of radix scutellariae powder, the capsule of weeping forsythia
20 parts of powder, 15 parts of kudzu-vine root powder, 12 parts of earthworm powder, 3.5 parts of polyethylene glycol oxide, 2 parts of polybutene acid ammonium, 0.7 part of brassinosteroid, shell
0.5 part of oligosaccharides；
Described compound bacteria is in units of parts by weight, including following raw material：35 parts of Bacillus, 28 parts of nitrobacteria, yeast
16 parts of bacterium, 8 parts of photosynthetic bacteria, 9 parts of rhizobium, 8 parts of phosphobacteria, 6 parts of B. mucilaginocus；
The preparation method of described bio-bacterial manure, comprises the following steps：
S1：Compound bacteria, radix scutellariae powder, capsule of weeping forsythia powder, kudzu-vine root powder, earthworm powder, polyethylene glycol oxide are mixed in proportion, in ultrasonic wave
Power is 250W, and temperature is to be fermented at 56 DEG C, ferments 5 days, zymotic fluid is made；
The preparation method of compound bacteria, comprises the following steps described in step S1：
A, respectively by Bacillus, nitrobacteria, saccharomycete, photosynthetic bacteria, rhizobium, phosphobacteria, B. mucilaginocus in proportion in temperature
Spend to be activated at 25 DEG C, expanding culture, with standby；
B, the Bacillus after culture, nitrobacteria, saccharomycete, photosynthetic bacteria, rhizobium, phosphobacteria, potassium will be expanded to step a
Bacterium is mixed and adds biological growth stimulant, biological nutritive agent, 600 parts of water are well mixed, and 16h is cultivated at being 27 DEG C in temperature
Afterwards, water removal is centrifuged in the case where rotating speed is 2500r/min, compound bacteria is made；The biological nutritive agent is in units of parts by weight, bag
Include following raw material：72 parts of yeast extract, 50 parts of sucrose, 26 parts of peptone, 25 parts of potassium acetate, 16 parts of sodium phosphate；The biological growth
Stimulant is in units of parts by weight, including following raw material：72 parts of cerium chloride, 40 parts of aluminum sulfate；
S2：Zymotic fluid made from step S1 is stood and abandons precipitation, and remaining ferment liquid is concentrated at 62 DEG C, is concentrated
Thing, it is 3% that the concentrate is dried to water content at 75 DEG C, and mixture is made；
S3：Mixture made from step S2 is crushed, it is few with polybutene acid ammonium, brassinosteroid, shell after 150 mesh sieve excessively
Sugar mixes in proportion, and the particle that particle diameter is 1.2mm is made, bio-bacterial manure is made.
Embodiment 3
A kind of bio-bacterial manure, in units of parts by weight, including following raw material：376 parts of compound bacteria, 28 parts of radix scutellariae powder, the capsule of weeping forsythia
24 parts of powder, 20 parts of kudzu-vine root powder, 15 parts of earthworm powder, 4 parts of polyethylene glycol oxide, 3 parts of polybutene acid ammonium, 0.8 part of brassinosteroid, shell are few
0.6 part of sugar；
Described compound bacteria is in units of parts by weight, including following raw material：40 parts of Bacillus, 35 parts of nitrobacteria, yeast
20 parts of bacterium, 10 parts of photosynthetic bacteria, 12 parts of rhizobium, 9 parts of phosphobacteria, 8 parts of B. mucilaginocus；
The preparation method of described bio-bacterial manure, comprises the following steps：
S1：Compound bacteria, radix scutellariae powder, capsule of weeping forsythia powder, kudzu-vine root powder, earthworm powder, polyethylene glycol oxide are mixed in proportion, in ultrasonic wave
Power is 300W, and temperature is to be fermented at 60 DEG C, ferments 4 days, zymotic fluid is made；
The preparation method of compound bacteria, comprises the following steps described in step S1：
A, respectively by Bacillus, nitrobacteria, saccharomycete, photosynthetic bacteria, rhizobium, phosphobacteria, B. mucilaginocus in proportion in temperature
Spend to be activated at 26 DEG C, expanding culture, with standby；
B, the Bacillus after culture, nitrobacteria, saccharomycete, photosynthetic bacteria, rhizobium, phosphobacteria, potassium will be expanded to step a
Bacterium is mixed and adds biological growth stimulant, biological nutritive agent, 650 parts of water are well mixed, and 13 are cultivated at being 28 DEG C in temperature
Afterwards, water removal is centrifuged in the case where rotating speed is 3000r/min, compound bacteria is made；The biological nutritive agent is in units of parts by weight, bag
Include following raw material：83 parts of yeast extract, 52 parts of sucrose, 28 parts of peptone, 26 parts of potassium acetate, 20 parts of sodium phosphate；The biological growth
Stimulant is in units of parts by weight, including following raw material：76 parts of cerium chloride, 42 parts of aluminum sulfate；
S2：Zymotic fluid made from step S1 is stood and abandons precipitation, and remaining ferment liquid is concentrated at 65 DEG C, is concentrated
Thing, it is 4.5% that the concentrate is dried to water content at 76 DEG C, and mixture is made；
S3：Mixture made from step S2 is crushed, it is few with polybutene acid ammonium, brassinosteroid, shell after 200 mesh sieve excessively
Sugar mixes in proportion, and the particle that particle diameter is 2mm is made, bio-bacterial manure is made.
Comparative example 1
It is essentially identical with the preparation technology of embodiment 2, have only the difference is that lacking in the raw material of preparation bio-bacterial manure compound
Bacterium, polyethylene glycol oxide, polybutene acid ammonium, brassinosteroid, chitosan oligosaccharide.
Comparative example 2
It is essentially identical with the preparation technology of embodiment 2, have only the difference is that lacking in the raw material of preparation bio-bacterial manure compound
Bacterium.
Comparative example 3
It is essentially identical with the preparation technology of embodiment 2, have only the difference is that lacking polyoxy in the raw material of preparation bio-bacterial manure
Change ethene.
Comparative example 4
It is essentially identical with the preparation technology of embodiment 2, have only the difference is that lacking poly- fourth in the raw material of preparation bio-bacterial manure
Olefin(e) acid ammonium.
Comparative example 5
It is essentially identical with the preparation technology of embodiment 2, have only the difference is that lacking rape in the raw material of preparation bio-bacterial manure
Plain lactone.
Comparative example 6
It is essentially identical with the preparation technology of embodiment 2, have only the difference is that lacking shell widow in the raw material of preparation bio-bacterial manure
Sugar.
Comparative example 7
Using a kind of Chinese patent application document " new bacterial manure (publication number：CN104446942A) " embodiment 1-3 work
Skill prepares bacterial manure.
With identical method, the corn of same breed is planted in Baise Tiandong County town, respectively using embodiment 1-3 and contrast
Organic fertilizer in example 1-7, per 1m21.5 kilogram of fertilizer are respectively applied in soil, after corn maturation, are taken equally amount respectively and appropriate are applied
The corn that the plantation of embodiment 1-3 and comparative example 1-7 organic fertilizer obtains, and the detection of indices is carried out, testing result is as follows
Shown in table.
As seen from the above table：(1) from embodiment 1-3 and the data of comparative example 7, using embodiment 1-3 bio-bacterial manures
Corn protein, the content of vitamin significantly improve, and resistance enhancing, quality improves；Simultaneously can by embodiment 1-3 data
See, embodiment 2 is optimum embodiment.
(2) from embodiment 2 and comparative example 1-6 data, compound bacteria, polyethylene glycol oxide, polybutene acid ammonium, rape element
Lactone, chitosan oligosaccharide serve synergy in bio-bacterial manure is prepared, and collaboration improves the content of corn protein, vitamin,
Wherein compound bacteria plays the effect of key in the content for improving corn protein, vitamin, and this is probably：
1) fertilizer made of compound bacteria has volume increase, the function of improving quality, also substantially reduces nitrate in plant,
Nitrite and content of beary metal, improve the effect such as chemical fertilizer utilization ratio and fertilizing soil.
2) polyethylene glycol oxide is water-wet side, can be combined with fertilizer particle, and polyethylene glycol oxide has good water resistance, and its
Strand can stretch well in water, uniformly be arranged in interface, increase steric hindrance, be quickly dispersed in fertilizer particle
In water.
3) polybutene acid ammonium can strengthen soil water-retaining performance, improved soil structure, the deep percolation for reducing water and soil and support
Shunting is lost, improves efficiency of water application.
4) division of the brassinosteroid to cell has obvious facilitation, can play swollen megalocarpous effect.It can beat
Broken apical dominance, promotes sprouting of lateral bud, can lead the differentiation of bud thoroughly, promotes side shoot generation, increases branch number, increase and spend number, improves
Becoming pregnant property of pollen, increase fruit number improve yield.
5) amino in chitosan oligosaccharide structure has the gentle control property of complexing, ion exchangeable, can be with fertilizer intermediate ion base
Group combines, and can not only be continuously plant and provide nutrient, long action time, moreover it is possible to excites defensin gene in plant, produces tool
There are chitinase, dextranase, guarantor's element and PR albumen of resistant effect etc., and with activating cell effect, contribute to aggrieved plant
Recovery, promoting root growth strong sprout, strengthen the resistance of crop, promote plant growing cycle must through the various stages, such as light and effect rank
Section etc., plant is obtained the amount of product by photosynthesis etc. in itself and maintain autologous growth enough.Chitosan oligosaccharide also have antivirus,
Bactericidal, fungicidal action, not only there is extremely strong preventing and treating and eradicant action to fungi, bacterium, virus, protect plant, make
The disease resistance of plant, the enhancing of anti-tolerance.Chitosan oligosaccharide is used cooperatively with brassinosteroid can improve plant in rape element
The absorption of ester, can promote Fruit, and and can promotes plant growth, flower and fruit protecting, prevents dehiscent fruit shedding, effectively change
The real quality of good fruit.
Above content is it cannot be assumed that present invention specific implementation is confined to these explanations, for the technical field of the invention
Those of ordinary skill for, under the premise of present inventive concept is not departed from, some simple deduction or replace can also be made, all should
When being considered as belonging to the scope of patent protection that is determined by the claims submitted of the present invention.
Claims (10)
1. a kind of bio-bacterial manure, it is characterised in that including following raw material：Compound bacteria, radix scutellariae powder, capsule of weeping forsythia powder, kudzu-vine root powder, earthworm
Powder, polyethylene glycol oxide, polybutene acid ammonium, brassinosteroid, chitosan oligosaccharide；
The compound bacteria, polyethylene glycol oxide, polybutene acid ammonium, brassinosteroid, weight ratio=(250-380) of chitosan oligosaccharide:(2-
4):(1.5-3):(0.5-0.8):(0.3-0.6)；
The preparation method of described bio-bacterial manure, comprises the following steps：
S1：Compound bacteria, radix scutellariae powder, capsule of weeping forsythia powder, kudzu-vine root powder, earthworm powder, polyethylene glycol oxide are mixed in proportion, in ul-trasonic irradiation
Under fermented, be made zymotic fluid；
S2：Zymotic fluid made from step S1 is stood and abandons precipitation, and remaining ferment liquid is concentrated, obtains concentrate, will be described dense
Contracting thing is dried, and mixture is made；
S3：Mixture made from step S2 is crushed, after sifting out with polybutene acid ammonium, brassinosteroid, chitosan oligosaccharide in proportion
It is well mixed, particle is made, bio-bacterial manure is made.
2. bio-bacterial manure according to claim 1, it is characterised in that described compound bacteria in units of parts by weight, including
Following raw material：Bacillus 25-40 parts, nitrobacteria 20-35 parts, saccharomycete 12-20 parts, photosynthetic bacteria 5-10 parts, rhizobium 6-12
Part, phosphobacteria 5-9 parts, B. mucilaginocus 4-8 parts.
3. bio-bacterial manure according to claim 2, it is characterised in that described compound bacteria in units of parts by weight, including
Following raw material：35 parts of Bacillus, 28 parts of nitrobacteria, 16 parts of saccharomycete, 8 parts of photosynthetic bacteria, 9 parts of rhizobium, 8 parts of phosphobacteria, potassium
6 parts of bacterium.
4. bio-bacterial manure according to claim 2, it is characterised in that fermented described in step S1 under ul-trasonic irradiation
Condition：It is 150-300W in ultrasonic power, temperature is to be fermented at 50-60 DEG C, is fermented 4-6 days.
5. bio-bacterial manure according to claim 2, it is characterised in that remaining zymotic fluid is at 54-65 DEG C described in step S2
Concentration.
6. bio-bacterial manure according to claim 2, it is characterised in that concentrate described in step S2 is done at 70-76 DEG C
It is dry.
7. bio-bacterial manure according to claim 6, it is characterised in that the drying to water content≤6%.
8. bio-bacterial manure according to claim 2, it is characterised in that sieve described in step S3 is 100-200 mesh.
9. bio-bacterial manure according to claim 2, it is characterised in that the particle diameter of particle described in step S3 is 0.1-2mm.
10. the application of a kind of bio-bacterial manure according to claim any one of 1-9, it is characterised in that beautiful applied to plantation
Meter Zhong.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112154759A (en) * | 2020-09-21 | 2021-01-01 | 陈文星 | Environment-friendly composite biological bacterial fertilizer |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104446942A (en) * | 2014-11-21 | 2015-03-25 | 苏州市吴中区光福香雪苗圃 | Novel bacterial fertilizer |
| CN105061062A (en) * | 2015-08-13 | 2015-11-18 | 李志山 | Special-effect biological organic fertilizer and preparation method thereof |
-
2017
- 2017-10-25 CN CN201711016153.5A patent/CN107556120A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104446942A (en) * | 2014-11-21 | 2015-03-25 | 苏州市吴中区光福香雪苗圃 | Novel bacterial fertilizer |
| CN105061062A (en) * | 2015-08-13 | 2015-11-18 | 李志山 | Special-effect biological organic fertilizer and preparation method thereof |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112154759A (en) * | 2020-09-21 | 2021-01-01 | 陈文星 | Environment-friendly composite biological bacterial fertilizer |
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