CN107551031A - A kind of Chinese medicine composition for alleviating kopiopia, include its eye sticker and preparation method - Google Patents
A kind of Chinese medicine composition for alleviating kopiopia, include its eye sticker and preparation method Download PDFInfo
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Abstract
The present invention provides a kind of Chinese medicine composition and eye sticker for alleviating kopiopia.The Chinese herb medicine eye paste provided by the invention for alleviating kopiopia, with chrysanthemum indicum, the shell of seaear and golden cypress altogether for monarch drug in a prescription, chrysanthemum indicum has clearing heat and detoxicating, the effect of clearing liver and improving vision, there is the shell of seaear soothing the liver to hold back courage, it is calming the liver the effect of, golden cypress has the effect of flat liver improving eyesight, chrysanthemum indicum, the shell of seaear and golden cypress compatibility, collaboration improve medicine flat liver improving eyesight the effect of;The present invention is using selfheal and moutan bark as ministerial drug, and selfheal has the effect of relieving inflammation or internal heat improving eyesight, mass dissipating and swelling eliminating, and moutan bark has the effect of anti-inflammation analgesia, and selfheal, moutan bark and monarch drug in a prescription compatibility can clear liver and improve vision, subside a swelling anti-inflammatory the effect of;The present invention is made using the red sage root, borneol and menthol as assistant, and red sage root promoting blood circulation, borneol, menthol lead to all keys, dissipates stagnated fire, swelling and pain relieving;All medicines share, altogether long memorial lead to eye channels and collaterals, accelerate eye circumference blood circulation, promote eye metabolism, promote to clear liver and improve vision, the effect of alleviating eye strain.
Description
Technical field
The invention belongs to field of health care products, and in particular to a kind of Chinese medicine composition for alleviating kopiopia, include its eye sticker
And preparation method.
Background technology
With the development of science and technology these electronic products such as mobile phone, computer are already be unable to do without in daily life.Due to
Long-term use of electronic product easily causes kopiopia with eye is of long duration.Kopiopia is a kind of ophthalmology common disease, caused by it
Eye it is dry, eye is puckery, eye is sour swollen, blurred vision even visual impairment directly affects the work and life of people.Kopiopia is mainly
When usually concentrating to see computer screen due to people, eyes number of winks is reduced, and is caused tears Secretion to reduce, is dodged simultaneously
Caused by bright fluorescent screen intense stimulus eyes.It can cause the corresponding sites such as the neck of people, shoulder pain occur, can also trigger and aggravate
Various illness in eye.
Alleviate the conventional method of kopiopia at present to be to maintain regular rest eyes, do eye exercises and using eye dropping of eye drops
Liquid, first two have certain alleviation visual fatigue, but people work, learning pressure rhythm is fast, do not ensure that regular rest
With do eye exercises;And containing preservative in eye dropping of eye drops liquid mostly, side effect occurs in long-term use.Have in recent years not
Few Chinese medicine eye paste application patent of invention, but the multi-component approach prepared is had nothing in common with each other, effect also differs.
The content of the invention
In order to effectively alleviate kopiopia, convenient use, the present invention provide a kind of Chinese medicine composition for alleviating kopiopia and
Include the eye sticker of the application Chinese medicine composition, and the preparation method of eye sticker.
It is an object of the invention to provide a kind of Chinese medicine composition for alleviating kopiopia.
It is a further object of the present invention to provide a kind of Chinese herb medicine eye paste for alleviating kopiopia.
It is to provide a kind of preparation method for the Chinese herb medicine eye paste for alleviating kopiopia another mesh of the present invention.
According to the Chinese medicine composition of the alleviation kopiopia of the specific embodiment of the invention, the Chinese medicine composition includes following
The raw material of parts by weight:
Selfheal 1-70 parts;
Shell of seaear 1-20 parts;
Chrysanthemum indicum 1-50 parts;
Red sage root 1-30 parts;
Moutan bark 1-30 parts;
With golden cypress 1-30 parts.
According to the Chinese medicine composition of the alleviation kopiopia of the specific embodiment of the invention, it is preferable that the Chinese medicine composition
Raw material also include:Borneol 1-130 parts and menthol 1-70 parts.
According to the Chinese herb medicine eye paste of the alleviation kopiopia of the specific embodiment of the invention, the Chinese herb medicine eye paste is by including following heavy
It is prepared by the Chinese medicine composition for measuring part:
Selfheal 1-70 parts;
Shell of seaear 1-20 parts;
Chrysanthemum indicum 1-50 parts;
Red sage root 1-30 parts;
Moutan bark 1-30 parts;
Golden cypress 1-30 parts;
Borneol 1-130 parts;
With menthol 1-70 parts.
According to the preparation method of the Chinese herb medicine eye paste of the alleviation kopiopia of the specific embodiment of the invention, methods described include with
Lower step:
(1) plant extraction liquid is prepared:Selfheal, the shell of seaear, chrysanthemum indicum, the red sage root, moutan bark and Huang are weighed according to parts by weight
Cypress, crush, sieving, add the water of 800-1500 parts by weight, steam distillation collects distillate, and the weight of the distillate is
0.4-0.6 times of the weight of the water, concentration, obtains plant extraction liquid;
(2) prepared plant extracts:The remaining mixture of distillate is collected in filtration step (1), is concentrated into paste, is added
Ethanol, staticly settle, filter, dry, obtain plant extracts;
(3) dry, crush the borneol and menthol, obtain mixed powder, the plant that the mixed powder and step (2) are obtained
Thing extract is added in the first plant extraction liquid that step (1) obtains, and is stirred, and is well mixed, and is filtered, and sterilizing, obtains Chinese medicine
Eye sticker liquid;
(4) non-woven fabrics is put into the Chinese herb medicine eye paste liquid that step (3) obtains and infiltrated, packed, obtain the alleviation kopiopia
Chinese herb medicine eye paste.
According to the preparation method of the Chinese herb medicine eye paste of the alleviation kopiopia of the specific embodiment of the invention, it is preferable that step (1)
In, the raw material that crushed is crossed into 100-200 mesh sieves.
According to the preparation method of the Chinese herb medicine eye paste of the alleviation kopiopia of the specific embodiment of the invention, it is preferable that step (1)
In, it is 150-300 parts by weight that the distillate being collected into is concentrated into weight.
According to the preparation method of the Chinese herb medicine eye paste of the alleviation kopiopia of the specific embodiment of the invention, it is preferable that step (2)
In, the volume fraction of the addition of the ethanol to ethanol in the solution is 50-60%.
According to the preparation method of the Chinese herb medicine eye paste of the alleviation kopiopia of the specific embodiment of the invention, it is preferable that step (2)
In, the condition that staticly settles is:Temperature is 20-30 DEG C, time 24-48h.
According to the preparation method of the Chinese herb medicine eye paste of the alleviation kopiopia of the specific embodiment of the invention, it is preferable that step (3)
In, it is 200-400 mesh that the borneol and menthol are crushed into particle diameter.
According to the preparation method of the Chinese herb medicine eye paste of the alleviation kopiopia of the specific embodiment of the invention, it is preferable that step (3)
In, the speed of the stirring is 1000-2000r/min, mixing time 10-20min.
In order to make it easy to understand, the present invention does progressive explanation to the effect of part material medicinal material.
Selfheal (scientific name:Prunella vulgaris L), containing triterpenoid saponin, its glucoside member is oleanolic acid.Also containing trip
From oleanolic acid, ursolic acid, rutin, hyperin, along caffeic acid, anti-caffeic acid, tannin, volatile oil, a small amount of alkaloid
And water soluble salt (predominantly potassium chloride).Anthocyanin, d- camphors, d- fennel fruit mattresses containing delphinidin and Cyanidin in flower spike
Ketone musk etc..It is cold in nature, it is bitter, pungent;Return liver, gallbladder channel.Major function:Relieve inflammation or internal heat, improving eyesight, dissipating bind, detumescence.For red eye, swell pain, mesh pearl
Nyctalgia, dizziness of having a headache, scrofula, goiter and tumor, swelling and pain;Thyroid Gland Swell, scrofula, the proliferation of mammary gland, hypertension.
Concha Haliotidis extract, (Classification system:Haliotis Diversicolor Reeve), taste is salty, puts down, nontoxic.Argument
Hide from view bitterly, glaucoma.Long term usage strengthening the essence is made light of one's life by commiting suicide.The shell of seaear, calming the liver, heat-clearing improving eyesight effect is stronger, kind to control mesh caused by liver-fire flaming
Red swelling and pain, vertigo.
Flos Chrysanthemi Indici extract is feverfew mother chrysanthemum Chrysanthemum indicum L., HERBA CHRYSANTHEMI LAVANDULAEFOLII C.boreale
Mak. or lavandulaleaf dendranthema herb C.lavandulaefolium (Fisch.) Mak. capitulum, have anti-inflammatory, anti-aging, prevent powder
Thorn, clearing heat and detoxicating and other effects.Containing volatile oil 01%~0.2%, contain chrysol (chrysol), chrysanthone in oil
(chrysantone), still camphor containing dl-, β -3- carenes (β -3-carene), sabinene (sabinene) and vanillyl alcohol
(carvol).In addition, yejuhua lactone (yejuhualactone) about 0.1%, butterflybush flower glucoside (acacetin- are separated to obtain in spending
7-rhamnoglucoside) about 0.72%.Another glycoside containing cyanidenon (luteolin glucoside), the red glucoside of chrysanthemum
(chrysanthemin asterin) chrysanthemaxanthin (chrysanthemaxanthin), epoxy lutein
(xanthophyllepoxide), cyanidenon, chrysanthemum indicum ketone (myrrh alkanone glycol), and chrysanthemol, Daucosterol, artemisiifolia
Element, acacetin, mountain Yu's acid glyceride, palmitic acid etc..
The red sage root, bitter, gas are slightly cold, nontoxic.Enter the heart, spleen two passes through.Special menstruation regulating arteries and veins, reason iliacus are ached, and raw new blood, remove extravesated blood.
Contain tanshinone IIA, tanshin polyphenolic acid B, Cryptotanshinone in the red sage root:Mainly there are fat-soluble non-quinochrome class compound, such as Tanshinone II
A, tanshinone ⅡB, Cryptotanshinone and its isomers.Water-soluble phenolic acrylic component such as protocatechualdehyde, danshensu.Wherein, the hidden red sage root
Ketone is the active ingredient of antibacterial.
Paeonol is mainly contained in Cortex Moutan extract, there is the effect such as analgesia, hemostasis, antibacterial.
Phellodendron extract, Classification system:Cortex Phellodendri Chinensis, phellodendron extract refer to from rue
The object extracted in the dry bark of savoury herb wampee or cork tree.It can specially anneal antipyretic, quench one's thirst and most imitate, go intestines wind, stop blooding
Dysentery, by bladder knot heat, leukorrhea with bloody discharge is controlled, rush down phase fire in kidney, also can flat liver improving eyesight.
The Chinese medicine composition for alleviating kopiopia and Chinese herb medicine eye paste provided by the invention are applied to due to reasons such as excess eye-usings
Caused kopiopia, it can equally be well applied to the daily nursing of person in middle and old age's ophthalmic uncomfortable.
Beneficial effects of the present invention are:
1. the Chinese herb medicine eye paste provided by the invention for alleviating kopiopia, with chrysanthemum indicum, the shell of seaear and golden cypress altogether for monarch drug in a prescription, mother chrysanthemum
Flower has clearing heat and detoxicating, and the effect of clearing liver and improving vision, there is the shell of seaear soothing the liver to hold back courage, it is calming the liver the effect of, golden cypress has soothing the liver
The effect of improving eyesight, chrysanthemum indicum, the shell of seaear and golden cypress compatibility, collaboration improve medicine flat liver improving eyesight the effect of.The present invention is withered with the summer
Grass and moutan bark are ministerial drug, and selfheal has the effect of improving eyesight of relieving inflammation or internal heat, mass dissipating and swelling eliminating, and moutan bark has anti-inflammation analgesic work(
Effect, selfheal, moutan bark and monarch drug in a prescription compatibility can clear liver and improve vision, the effect of functions of detumescence, relieving inflammation;The present invention is with the red sage root, borneol and peppermint
Brain makes for assistant, and red sage root promoting blood circulation, borneol, menthol lead to all keys, dissipates stagnated fire, swelling and pain relieving;All medicines share, common long memorial lead to eye
Channels and collaterals, accelerate eye circumference blood circulation, promote eye metabolism, promote to clear liver and improve vision, alleviate eye strain the effect of.
2. the method provided by the invention for preparing the Chinese herb medicine eye paste for alleviating kopiopia, by steam distillation by raw material
Volatile ingredient extract, then using alcohol precipitation by remaining active ingredient in raw material carry out clean extraction obtain medicine original
Active ingredient in material, while go the removal of impurity, there is provided the content of effective ingredient, at utmost retain effective ingredient.
Embodiment
To make the object, technical solutions and advantages of the present invention clearer, technical scheme will be carried out below
Detailed description.Obviously, described embodiment is only part of the embodiment of the present invention, rather than whole embodiments.Base
Embodiment in the present invention, those of ordinary skill in the art are resulting on the premise of creative work is not made to be owned
Other embodiment, belong to the scope that the present invention is protected.
Embodiment 1
Alleviate the Chinese medicine composition of kopiopia, the Chinese medicine composition includes the raw material of following weight:Selfheal 10g;Stone
Cassia 10g;Chrysanthemum indicum 10g;Red sage root 10g;Moutan bark 10g and golden cypress 10g.
Embodiment 2
Alleviate the Chinese medicine composition of kopiopia, the Chinese medicine composition includes the raw material of following weight:Selfheal 700g;Stone
Cassia 200g;Chrysanthemum indicum 500g;Red sage root 300g;Moutan bark 300g and golden cypress 300g.
Embodiment 3
Alleviate the Chinese medicine composition of kopiopia, the Chinese medicine composition includes the raw material of following weight:Selfheal 700g;Stone
Cassia 200g;Chrysanthemum indicum 500g;Red sage root 300g;Moutan bark 300g;Golden cypress 300g;Borneol 1300g and menthol 700g.
Embodiment 4
Alleviate the Chinese medicine composition of kopiopia, the Chinese medicine composition includes the raw material of following weight:Selfheal 100g;Stone
Cassia 50g;Chrysanthemum indicum 100g;Red sage root 50g;Moutan bark 50g;Golden cypress 50g;Borneol 10g and menthol 10g.
Embodiment 5
Alleviate the Chinese herb medicine eye paste of kopiopia, the Chinese herb medicine eye paste is prepared by the Chinese medicine composition including following weight:Summer is withered
Careless 10g;Shell of seaear 10g;Chrysanthemum indicum 10g;Red sage root 10g;Moutan bark 10g;Golden cypress 10g borneols 10g and menthol 10g.
The preparation method of the Chinese herb medicine eye paste of above-mentioned alleviation kopiopia, the described method comprises the following steps:
(1) plant extraction liquid is prepared:Selfheal, the shell of seaear, chrysanthemum indicum, the red sage root, moutan bark and Huang are weighed according to parts by weight
Cypress, crushing, cross 100 mesh sieves, add 800g water, steam distillation, collect distillate, the weight of the distillate is 320g,
150g is concentrated into, obtains plant extraction liquid;
(2) prepared plant extracts:The remaining mixture of distillate is collected in filtration step (1), is concentrated into paste, is added
Volume fraction of the ethanol to ethanol in the solution is 50%, staticly settles 24h at 20 DEG C, filters, dries, obtain plant extract
Thing;
(3) it is 200 mesh to dry, crush the borneol and menthol to particle diameter, mixed powder is obtained, by the mixed powder and step
Suddenly the plant extracts that (2) obtain is added in the first plant extraction liquid that step (1) obtains, and is stirred with 1000r/min speed
20min is mixed, is well mixed, is filtered, sterilizing, obtains Chinese herb medicine eye paste liquid;
(4) non-woven fabrics is put into the Chinese herb medicine eye paste liquid that step (3) obtains and infiltrated, packed, obtain the alleviation kopiopia
Chinese herb medicine eye paste.
Embodiment 6
Alleviate the Chinese herb medicine eye paste of kopiopia, the Chinese herb medicine eye paste is prepared by the Chinese medicine composition including following weight:Summer is withered
Careless 70g;Shell of seaear 20g;Chrysanthemum indicum 50g;Red sage root 30g;Moutan bark 30g;Golden cypress 30g;Borneol 130g and menthol 70g.
The preparation method of the Chinese herb medicine eye paste of above-mentioned alleviation kopiopia, the described method comprises the following steps:
(1) plant extraction liquid is prepared:Selfheal, the shell of seaear, chrysanthemum indicum, the red sage root, moutan bark and Huang are weighed according to parts by weight
Cypress, crushing, cross 200 mesh sieves, add 1500g water, steam distillation, collect distillate, the weight of the distillate is 900g,
300g is concentrated into, obtains plant extraction liquid;
(2) prepared plant extracts:The remaining mixture of distillate is collected in filtration step (1), is concentrated into paste, is added
Volume fraction of the ethanol to ethanol in the solution is 60%, staticly settles 48h at 30 DEG C, filters, dries, obtain plant extract
Thing;
(3) it is 400 mesh to dry, crush the borneol and menthol to particle diameter, mixed powder is obtained, by the mixed powder and step
Suddenly the plant extracts that (2) obtain is added in the first plant extraction liquid that step (1) obtains, and is stirred with 2000r/min speed
10min is mixed, is well mixed, is filtered, sterilizing, obtains Chinese herb medicine eye paste liquid;
(4) non-woven fabrics is put into the Chinese herb medicine eye paste liquid that step (3) obtains and infiltrated, packed, obtain the alleviation kopiopia
Chinese herb medicine eye paste.
Embodiment 7
Alleviate the Chinese herb medicine eye paste of kopiopia, the Chinese herb medicine eye paste is prepared by the Chinese medicine composition including following weight:Summer is withered
Careless 50g;Shell of seaear 10g;Chrysanthemum indicum 25g;Red sage root 15g;Moutan bark 15g;Golden cypress 15g;Borneol 30g and menthol 45g.
The preparation method of the Chinese herb medicine eye paste of above-mentioned alleviation kopiopia, the described method comprises the following steps:
(1) plant extraction liquid is prepared:Selfheal, the shell of seaear, chrysanthemum indicum, the red sage root, moutan bark and Huang are weighed according to parts by weight
Cypress, crushing, cross 200 mesh sieves, add 1000g water, steam distillation, collect distillate, the weight of the distillate is 500g,
250g is concentrated into, obtains plant extraction liquid;
(2) prepared plant extracts:The remaining mixture of distillate is collected in filtration step (1), is concentrated into paste, is added
Volume fraction of the ethanol to ethanol in the solution is 60%, staticly settles 48h at 25 DEG C, filters, dries, obtain plant extract
Thing;
(3) it is 400 mesh to dry, crush the borneol and menthol to particle diameter, mixed powder is obtained, by the mixed powder and step
Suddenly the plant extracts that (2) obtain is added in the first plant extraction liquid that step (1) obtains, and is stirred with 2000r/min speed
15min is mixed, is well mixed, is filtered, sterilizing, obtains Chinese herb medicine eye paste liquid;
(4) non-woven fabrics is put into the Chinese herb medicine eye paste liquid that step (3) obtains and infiltrated, packed, obtain the alleviation kopiopia
Chinese herb medicine eye paste.
Test example
The effect of Chinese herb medicine eye paste of the alleviation kopiopia obtained to the embodiment of the present invention 7, is detected.
Subject:Screening is 150 long-term to be faced computer, sees that TV, driving vehicle, the trial volunteer of writing are carried out for a long time
Test, the people of acceptance test generally have the one or more in the symptoms such as eye is dry, eye is puckery, ophthalmodynia, eye is swollen, eye-blurred.
Application method:Before use, face is cleaned with clear water, emphasis cleaning eye circumference, is then obtained the embodiment of the present invention 7
The Chinese herb medicine eye paste of alleviation kopiopia be externally applied to eye circumference, stick 10-15min, massage eye circumference 1min or so to drug absorption, daily
1 time, one month is a course for the treatment of.
Trial volunteer is randomly divided into three groups, every group of 50 people, one of which is test group, and one group is control group, one group
For blank group, the trial volunteer of test group uses Chinese herb medicine eye paste provided by the invention according to method provided by the invention, control
Group (is recorded, its main component is chrysanthemum indicum, golden cypress, pellet using certain commercially available eye sticker for alleviating kopiopia according to its specification daily
Ginseng, pearl, borneol and menthol), blank group without using.Result of the test is counted after one month.
Wherein test group kopiopia symptom compared with the subject of control group and blank group is obviously improved, test group it is tested
The eye of person is dry, eye is puckery, ophthalmodynia, eye is swollen, the symptom of eye-blurred disappears substantially, and improvement rate reaches 94%;The subject of control group
Ophthalmic uncomfortable make moderate progress, improvement rate 80%;Subject's ophthalmic uncomfortable symptom of blank group is substantially without improvement, or even has portion
Subject's ophthalmic uncomfortable is divided to aggravate, improvement rate 2%.The improvement rate is to be tested by one month, and subject's ophthalmic uncomfortable disappears
Lose, ophthalmic uncomfortable no longer occurs in deactivation symptom within one week is improver, the number of improver and this group of subject's total number of persons
The percentage of ratio be improvement rate.
Result of the test shows the Chinese herb medicine eye paste of alleviation kopiopia provided by the invention for for a long time with eye caused by eye is dry, eye
The symptoms such as puckery, ophthalmodynia, eye are swollen, eye-blurred have the effect of obvious.
The foregoing is only a specific embodiment of the invention, but protection scope of the present invention is not limited thereto, any
Those familiar with the art the invention discloses technical scope in, change or replacement can be readily occurred in, should all be contained
Cover within protection scope of the present invention.Therefore, protection scope of the present invention should be based on the protection scope of the described claims.
Claims (10)
1. a kind of Chinese medicine composition for alleviating kopiopia, it is characterised in that the Chinese medicine composition includes the original of following parts by weight
Material:
Selfheal 1-70 parts;
Shell of seaear 1-20 parts;
Chrysanthemum indicum 1-50 parts;
Red sage root 1-30 parts;
Moutan bark 1-30 parts;
With golden cypress 1-30 parts.
2. the Chinese medicine composition according to claim 1 for alleviating kopiopia, it is characterised in that the original of the Chinese medicine composition
Material also includes:Borneol 1-130 parts and menthol 1-70 parts.
3. a kind of Chinese herb medicine eye paste for alleviating kopiopia, it is characterised in that the Chinese herb medicine eye paste is as including slow described in claim 2
It is prepared by the Chinese medicine composition for solving kopiopia.
A kind of 4. method of the Chinese herb medicine eye paste of the alleviation kopiopia prepared described in claim 3, it is characterised in that methods described bag
Include following steps:
(1) plant extraction liquid is prepared:Selfheal, the shell of seaear, chrysanthemum indicum, the red sage root, moutan bark and golden cypress, powder are weighed according to parts by weight
It is broken, sieving, the water of 800-1500 parts by weight is added, steam distillation, collects distillate, the weight of the distillate is the water
0.4-0.6 times of weight, concentration, obtain plant extraction liquid;
(2) prepared plant extracts:The remaining mixture of distillate is collected in filtration step (1), is concentrated into paste, adds second
Alcohol, staticly settle, filter, dry, obtain plant extracts;
(3) dry, crush the borneol and menthol, obtain mixed powder, the plant that the mixed powder and step (2) obtain is carried
Take thing to be added in the first plant extraction liquid that step (1) obtains, stir, be well mixed, filter, sterilizing, obtain Chinese herb medicine eye paste
Liquid;
(4) non-woven fabrics is put into the Chinese herb medicine eye paste liquid that step (3) obtains and infiltrated, packed, obtained in the alleviation kopiopia
Medicine eye sticker.
5. the method according to claim 4 for preparing the Chinese herb medicine eye paste for alleviating kopiopia, it is characterised in that in step (1),
The raw material that crushed is crossed into 100-200 mesh sieves.
6. the method according to claim 4 for preparing the Chinese herb medicine eye paste for alleviating kopiopia, it is characterised in that in step (1),
It is 150-300 parts by weight that the distillate of collection is concentrated into weight.
7. the method according to claim 4 for preparing the Chinese herb medicine eye paste for alleviating kopiopia, it is characterised in that in step (2),
Volume fraction of the addition of the ethanol to ethanol in the solution is 50-60%.
8. the method according to claim 4 for preparing the Chinese herb medicine eye paste for alleviating kopiopia, it is characterised in that in step (2),
The condition that staticly settles is:Temperature is 20-30 DEG C, time 24-48h.
9. the method according to claim 4 for preparing the Chinese herb medicine eye paste for alleviating kopiopia, it is characterised in that in step (3),
It is 200-400 mesh that the borneol and menthol are crushed into particle diameter.
10. the method according to claim 4 for preparing the Chinese herb medicine eye paste for alleviating kopiopia, it is characterised in that step (3)
In, the speed of the stirring is 1000-2000r/min, mixing time 10-20min.
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