CN107532166A - 用于减少非特异性扩增产物的方法和组合物 - Google Patents

用于减少非特异性扩增产物的方法和组合物 Download PDF

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Publication number
CN107532166A
CN107532166A CN201680021382.3A CN201680021382A CN107532166A CN 107532166 A CN107532166 A CN 107532166A CN 201680021382 A CN201680021382 A CN 201680021382A CN 107532166 A CN107532166 A CN 107532166A
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target
dna
specific amplification
specific
amplification product
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Chinese (zh)
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刘至东
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Bailong Gene Co
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Bailong Gene Co
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Priority to CN202510844827.9A priority Critical patent/CN120700118A/zh
Priority to CN202510844889.XA priority patent/CN120700119A/zh
Publication of CN107532166A publication Critical patent/CN107532166A/zh
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6846—Common amplification features
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/30—Phosphoric diester hydrolysing, i.e. nuclease
    • C12Q2521/301—Endonuclease
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/191—Modifications characterised by incorporating an adaptor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/101—Temperature
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
    • C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
    • C12Q2537/143—Multiplexing, i.e. use of multiple primers or probes in a single reaction, usually for simultaneously analyse of multiple analysis

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Analytical Chemistry (AREA)
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  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
CN201680021382.3A 2015-02-11 2016-02-11 用于减少非特异性扩增产物的方法和组合物 Pending CN107532166A (zh)

Priority Applications (2)

Application Number Priority Date Filing Date Title
CN202510844827.9A CN120700118A (zh) 2015-02-11 2016-02-11 用于减少非特异性扩增产物的方法和组合物
CN202510844889.XA CN120700119A (zh) 2015-02-11 2016-02-11 用于减少非特异性扩增产物的方法和组合物

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201562114788P 2015-02-11 2015-02-11
US62/114,788 2015-02-11
US201562150600P 2015-04-21 2015-04-21
US62/150,600 2015-04-21
PCT/US2016/017454 WO2016130746A1 (en) 2015-02-11 2016-02-11 Method and compositions for reducing non-specific amplification products

Related Child Applications (2)

Application Number Title Priority Date Filing Date
CN202510844889.XA Division CN120700119A (zh) 2015-02-11 2016-02-11 用于减少非特异性扩增产物的方法和组合物
CN202510844827.9A Division CN120700118A (zh) 2015-02-11 2016-02-11 用于减少非特异性扩增产物的方法和组合物

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CN107532166A true CN107532166A (zh) 2018-01-02

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CN201680021382.3A Pending CN107532166A (zh) 2015-02-11 2016-02-11 用于减少非特异性扩增产物的方法和组合物
CN202510844889.XA Pending CN120700119A (zh) 2015-02-11 2016-02-11 用于减少非特异性扩增产物的方法和组合物
CN202510844827.9A Pending CN120700118A (zh) 2015-02-11 2016-02-11 用于减少非特异性扩增产物的方法和组合物

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CN202510844827.9A Pending CN120700118A (zh) 2015-02-11 2016-02-11 用于减少非特异性扩增产物的方法和组合物

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US (1) US9464318B2 (enExample)
EP (1) EP3256584B1 (enExample)
JP (1) JP6755890B2 (enExample)
KR (1) KR20170110721A (enExample)
CN (3) CN107532166A (enExample)
AU (1) AU2016219288B2 (enExample)
BR (1) BR112017017142B1 (enExample)
CA (1) CA2976251C (enExample)
ES (1) ES2795404T3 (enExample)
WO (1) WO2016130746A1 (enExample)

Cited By (3)

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CN114369649A (zh) * 2022-02-08 2022-04-19 山东见微生物科技有限公司 一种特异的选择扩增及多重pcr方法和应用
CN114774526A (zh) * 2022-04-25 2022-07-22 陕西科技大学 一种dna长度的熔解测定方法及试剂盒
CN114875119A (zh) * 2021-02-05 2022-08-09 广东菲鹏生物有限公司 一种提高核酸扩增效率的方法及其应用

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US10421993B2 (en) 2015-02-11 2019-09-24 Paragon Genomics, Inc. Methods and compositions for reducing non-specific amplification products
CN107058573B (zh) * 2016-06-13 2020-07-10 艾吉泰康生物科技(北京)有限公司 一种利用Cas9/gRNA系统构建扩增子文库的方法
CN110382716A (zh) * 2017-01-10 2019-10-25 佰隆基因公司 用于减少引物延伸反应中产生的冗余分子条形码的方法和组合物
WO2018140329A1 (en) * 2017-01-24 2018-08-02 Tsavachidou Dimitra Methods for constructing copies of nucleic acid molecules
CN111936635B (zh) * 2018-03-02 2024-08-23 豪夫迈·罗氏有限公司 用于单分子测序的单链环状dna模板的产生
US11976275B2 (en) 2018-06-15 2024-05-07 Kapa Biosystems, Inc. Generation of double-stranded DNA templates for single molecule sequencing
WO2020145351A1 (ja) * 2019-01-09 2020-07-16 国立研究開発法人理化学研究所 薬物動態関連遺伝子の網羅的配列解析法とそれに使用されるプライマーセット
US11396674B2 (en) * 2019-02-22 2022-07-26 Gianluca ROMA Multiplex nucleic acid amplification and library preparation
US10941453B1 (en) 2020-05-20 2021-03-09 Paragon Genomics, Inc. High throughput detection of pathogen RNA in clinical specimens
US11680293B1 (en) 2022-04-21 2023-06-20 Paragon Genomics, Inc. Methods and compositions for amplifying DNA and generating DNA sequencing results from target-enriched DNA molecules
US12091715B2 (en) 2022-04-21 2024-09-17 Paragon Genomics, Inc. Methods and compositions for reducing base errors of massive parallel sequencing using triseq sequencing

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114875119A (zh) * 2021-02-05 2022-08-09 广东菲鹏生物有限公司 一种提高核酸扩增效率的方法及其应用
CN114369649A (zh) * 2022-02-08 2022-04-19 山东见微生物科技有限公司 一种特异的选择扩增及多重pcr方法和应用
CN114774526A (zh) * 2022-04-25 2022-07-22 陕西科技大学 一种dna长度的熔解测定方法及试剂盒

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CA2976251C (en) 2023-08-22
BR112017017142A2 (pt) 2018-04-03
CA2976251A1 (en) 2016-08-18
CN120700119A (zh) 2025-09-26
EP3256584A1 (en) 2017-12-20
JP6755890B2 (ja) 2020-09-16
AU2016219288A1 (en) 2017-08-17
US20160230222A1 (en) 2016-08-11
EP3256584B1 (en) 2020-03-25
AU2016219288B2 (en) 2021-10-28
BR112017017142B1 (pt) 2024-03-12
US9464318B2 (en) 2016-10-11
CN120700118A (zh) 2025-09-26
JP2018506307A (ja) 2018-03-08
ES2795404T3 (es) 2020-11-23
WO2016130746A1 (en) 2016-08-18
KR20170110721A (ko) 2017-10-11
EP3256584A4 (en) 2018-08-15

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