BR112017017142B1 - Método para redução dos produtos de amplificação não específicos a partir de uma reação de extensão de iniciador dependente de molde - Google Patents
Método para redução dos produtos de amplificação não específicos a partir de uma reação de extensão de iniciador dependente de molde Download PDFInfo
- Publication number
- BR112017017142B1 BR112017017142B1 BR112017017142-2A BR112017017142A BR112017017142B1 BR 112017017142 B1 BR112017017142 B1 BR 112017017142B1 BR 112017017142 A BR112017017142 A BR 112017017142A BR 112017017142 B1 BR112017017142 B1 BR 112017017142B1
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- Prior art keywords
- amplification products
- dna
- target
- specific amplification
- specific
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6846—Common amplification features
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2521/00—Reaction characterised by the enzymatic activity
- C12Q2521/30—Phosphoric diester hydrolysing, i.e. nuclease
- C12Q2521/301—Endonuclease
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
- C12Q2525/10—Modifications characterised by
- C12Q2525/191—Modifications characterised by incorporating an adaptor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2527/00—Reactions demanding special reaction conditions
- C12Q2527/101—Temperature
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
- C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
- C12Q2537/143—Multiplexing, i.e. use of multiple primers or probes in a single reaction, usually for simultaneously analyse of multiple analysis
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Analytical Chemistry (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Immunology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Physics & Mathematics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201562114788P | 2015-02-11 | 2015-02-11 | |
| US62/114,788 | 2015-02-11 | ||
| US201562150600P | 2015-04-21 | 2015-04-21 | |
| US62/150,600 | 2015-04-21 | ||
| PCT/US2016/017454 WO2016130746A1 (en) | 2015-02-11 | 2016-02-11 | Method and compositions for reducing non-specific amplification products |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| BR112017017142A2 BR112017017142A2 (en) | 2018-04-03 |
| BR112017017142B1 true BR112017017142B1 (pt) | 2024-03-12 |
Family
ID=56566583
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BR112017017142-2A BR112017017142B1 (pt) | 2015-02-11 | 2016-02-11 | Método para redução dos produtos de amplificação não específicos a partir de uma reação de extensão de iniciador dependente de molde |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US9464318B2 (enExample) |
| EP (1) | EP3256584B1 (enExample) |
| JP (1) | JP6755890B2 (enExample) |
| KR (1) | KR20170110721A (enExample) |
| CN (3) | CN120700119A (enExample) |
| AU (1) | AU2016219288B2 (enExample) |
| BR (1) | BR112017017142B1 (enExample) |
| CA (1) | CA2976251C (enExample) |
| ES (1) | ES2795404T3 (enExample) |
| WO (1) | WO2016130746A1 (enExample) |
Families Citing this family (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10421993B2 (en) | 2015-02-11 | 2019-09-24 | Paragon Genomics, Inc. | Methods and compositions for reducing non-specific amplification products |
| CN107058573B (zh) * | 2016-06-13 | 2020-07-10 | 艾吉泰康生物科技(北京)有限公司 | 一种利用Cas9/gRNA系统构建扩增子文库的方法 |
| CN110382716A (zh) * | 2017-01-10 | 2019-10-25 | 佰隆基因公司 | 用于减少引物延伸反应中产生的冗余分子条形码的方法和组合物 |
| CN110382710B (zh) * | 2017-01-24 | 2024-10-01 | 瓦斯特根公司 | 构建核酸分子拷贝的方法 |
| WO2019166565A1 (en) * | 2018-03-02 | 2019-09-06 | F. Hoffmann-La Roche Ag | Generation of double-stranded dna templates for single molecule sequencing |
| US11976275B2 (en) | 2018-06-15 | 2024-05-07 | Kapa Biosystems, Inc. | Generation of double-stranded DNA templates for single molecule sequencing |
| WO2020145351A1 (ja) * | 2019-01-09 | 2020-07-16 | 国立研究開発法人理化学研究所 | 薬物動態関連遺伝子の網羅的配列解析法とそれに使用されるプライマーセット |
| US11396674B2 (en) * | 2019-02-22 | 2022-07-26 | Gianluca ROMA | Multiplex nucleic acid amplification and library preparation |
| US10941453B1 (en) | 2020-05-20 | 2021-03-09 | Paragon Genomics, Inc. | High throughput detection of pathogen RNA in clinical specimens |
| CN114875119B (zh) * | 2021-02-05 | 2025-11-18 | 广东菲鹏生物有限公司 | 一种提高核酸扩增产物浓度的方法及其应用 |
| CN114369649A (zh) * | 2022-02-08 | 2022-04-19 | 山东见微生物科技有限公司 | 一种特异的选择扩增及多重pcr方法和应用 |
| US11680293B1 (en) | 2022-04-21 | 2023-06-20 | Paragon Genomics, Inc. | Methods and compositions for amplifying DNA and generating DNA sequencing results from target-enriched DNA molecules |
| US12091715B2 (en) | 2022-04-21 | 2024-09-17 | Paragon Genomics, Inc. | Methods and compositions for reducing base errors of massive parallel sequencing using triseq sequencing |
| CN114774526B (zh) * | 2022-04-25 | 2025-12-12 | 陕西科技大学 | 一种dna长度的熔解测定方法及试剂盒 |
| CN121183047B (zh) * | 2025-11-24 | 2026-03-31 | 湖南圣测生物科技有限公司 | 一种同时检测非洲猪瘟病毒、猪流行性腹泻病毒、猪繁殖与呼吸综合征病毒的引物探针组合、试剂盒及其应用 |
Family Cites Families (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5750341A (en) | 1995-04-17 | 1998-05-12 | Lynx Therapeutics, Inc. | DNA sequencing by parallel oligonucleotide extensions |
| US20040002156A1 (en) * | 2002-06-26 | 2004-01-01 | Stratagene | Selective cloning of homoduplex nucleic acids |
| DE602004026033D1 (de) * | 2003-01-29 | 2010-04-29 | 454 Corp | Zweiendige sequenzierung |
| EP1574570A1 (en) | 2004-03-12 | 2005-09-14 | Universität Regensburg | Process for reducing the number of mismatches in double stranded polynucleotides |
| WO2006023919A2 (en) * | 2004-08-24 | 2006-03-02 | Cornell Research Foundation, Inc. | Detection of nucleic acid differences using endonuclease cleavage/ligas releasing reactions and capillary electrophoresis or microarrays |
| US20060281113A1 (en) | 2005-05-18 | 2006-12-14 | George Church | Accessible polynucleotide libraries and methods of use thereof |
| US8716190B2 (en) * | 2007-09-14 | 2014-05-06 | Affymetrix, Inc. | Amplification and analysis of selected targets on solid supports |
| US9540637B2 (en) * | 2008-01-09 | 2017-01-10 | Life Technologies Corporation | Nucleic acid adaptors and uses thereof |
| US8586310B2 (en) | 2008-09-05 | 2013-11-19 | Washington University | Method for multiplexed nucleic acid patch polymerase chain reaction |
| US9957558B2 (en) | 2011-04-28 | 2018-05-01 | Life Technologies Corporation | Methods and compositions for multiplex PCR |
| EP2943590A4 (en) | 2013-01-13 | 2017-02-15 | Unitaq Bio | Methods and compositions for pcr using blocked and universal primers |
| WO2015063154A1 (en) | 2013-10-31 | 2015-05-07 | Multiplicom N.V. | Blocking primers in multiplex pcr based assays |
-
2016
- 2016-02-11 CN CN202510844889.XA patent/CN120700119A/zh active Pending
- 2016-02-11 BR BR112017017142-2A patent/BR112017017142B1/pt active IP Right Grant
- 2016-02-11 ES ES16749846T patent/ES2795404T3/es active Active
- 2016-02-11 CA CA2976251A patent/CA2976251C/en active Active
- 2016-02-11 EP EP16749846.8A patent/EP3256584B1/en active Active
- 2016-02-11 JP JP2017560886A patent/JP6755890B2/ja active Active
- 2016-02-11 WO PCT/US2016/017454 patent/WO2016130746A1/en not_active Ceased
- 2016-02-11 CN CN202510844827.9A patent/CN120700118A/zh active Pending
- 2016-02-11 US US15/041,644 patent/US9464318B2/en active Active
- 2016-02-11 AU AU2016219288A patent/AU2016219288B2/en active Active
- 2016-02-11 CN CN201680021382.3A patent/CN107532166A/zh active Pending
- 2016-02-11 KR KR1020177025384A patent/KR20170110721A/ko not_active Ceased
Also Published As
| Publication number | Publication date |
|---|---|
| BR112017017142A2 (en) | 2018-04-03 |
| KR20170110721A (ko) | 2017-10-11 |
| CA2976251A1 (en) | 2016-08-18 |
| EP3256584A1 (en) | 2017-12-20 |
| JP6755890B2 (ja) | 2020-09-16 |
| CN120700118A (zh) | 2025-09-26 |
| US9464318B2 (en) | 2016-10-11 |
| CN107532166A (zh) | 2018-01-02 |
| ES2795404T3 (es) | 2020-11-23 |
| EP3256584B1 (en) | 2020-03-25 |
| CA2976251C (en) | 2023-08-22 |
| WO2016130746A1 (en) | 2016-08-18 |
| JP2018506307A (ja) | 2018-03-08 |
| AU2016219288B2 (en) | 2021-10-28 |
| CN120700119A (zh) | 2025-09-26 |
| EP3256584A4 (en) | 2018-08-15 |
| AU2016219288A1 (en) | 2017-08-17 |
| US20160230222A1 (en) | 2016-08-11 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| B06U | Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette] | ||
| B06A | Patent application procedure suspended [chapter 6.1 patent gazette] | ||
| B09A | Decision: intention to grant [chapter 9.1 patent gazette] | ||
| B16A | Patent or certificate of addition of invention granted [chapter 16.1 patent gazette] |
Free format text: PRAZO DE VALIDADE: 20 (VINTE) ANOS CONTADOS A PARTIR DE 11/02/2016, OBSERVADAS AS CONDICOES LEGAIS |