CA2976251C - Methods and compositions for reducing non-specific amplification products - Google Patents
Methods and compositions for reducing non-specific amplification products Download PDFInfo
- Publication number
- CA2976251C CA2976251C CA2976251A CA2976251A CA2976251C CA 2976251 C CA2976251 C CA 2976251C CA 2976251 A CA2976251 A CA 2976251A CA 2976251 A CA2976251 A CA 2976251A CA 2976251 C CA2976251 C CA 2976251C
- Authority
- CA
- Canada
- Prior art keywords
- amplification products
- target
- specific amplification
- dna
- specific
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6846—Common amplification features
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2521/00—Reaction characterised by the enzymatic activity
- C12Q2521/30—Phosphoric diester hydrolysing, i.e. nuclease
- C12Q2521/301—Endonuclease
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
- C12Q2525/10—Modifications characterised by
- C12Q2525/191—Modifications characterised by incorporating an adaptor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2527/00—Reactions demanding special reaction conditions
- C12Q2527/101—Temperature
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
- C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
- C12Q2537/143—Multiplexing, i.e. use of multiple primers or probes in a single reaction, usually for simultaneously analyse of multiple analysis
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Analytical Chemistry (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Immunology (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201562114788P | 2015-02-11 | 2015-02-11 | |
| US62/114,788 | 2015-02-11 | ||
| US201562150600P | 2015-04-21 | 2015-04-21 | |
| US62/150,600 | 2015-04-21 | ||
| PCT/US2016/017454 WO2016130746A1 (en) | 2015-02-11 | 2016-02-11 | Method and compositions for reducing non-specific amplification products |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2976251A1 CA2976251A1 (en) | 2016-08-18 |
| CA2976251C true CA2976251C (en) | 2023-08-22 |
Family
ID=56566583
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2976251A Active CA2976251C (en) | 2015-02-11 | 2016-02-11 | Methods and compositions for reducing non-specific amplification products |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US9464318B2 (enExample) |
| EP (1) | EP3256584B1 (enExample) |
| JP (1) | JP6755890B2 (enExample) |
| KR (1) | KR20170110721A (enExample) |
| CN (3) | CN107532166A (enExample) |
| AU (1) | AU2016219288B2 (enExample) |
| BR (1) | BR112017017142B1 (enExample) |
| CA (1) | CA2976251C (enExample) |
| ES (1) | ES2795404T3 (enExample) |
| WO (1) | WO2016130746A1 (enExample) |
Families Citing this family (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10421993B2 (en) | 2015-02-11 | 2019-09-24 | Paragon Genomics, Inc. | Methods and compositions for reducing non-specific amplification products |
| CN107058573B (zh) * | 2016-06-13 | 2020-07-10 | 艾吉泰康生物科技(北京)有限公司 | 一种利用Cas9/gRNA系统构建扩增子文库的方法 |
| EP3568493B1 (en) * | 2017-01-10 | 2021-03-24 | Paragon Genomics, Inc. | Methods and compositions for reducing redundant molecular barcodes created in primer extension reactions |
| US11981961B2 (en) | 2017-01-24 | 2024-05-14 | Vastogen, Inc. | Methods for constructing copies of nucleic acid molecules |
| JP2021514646A (ja) * | 2018-03-02 | 2021-06-17 | エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ | 単一分子シーケンシングのための、二本鎖ｄｎａ鋳型の作出 |
| US11976275B2 (en) | 2018-06-15 | 2024-05-07 | Kapa Biosystems, Inc. | Generation of double-stranded DNA templates for single molecule sequencing |
| JP7626430B2 (ja) * | 2019-01-09 | 2025-02-04 | 国立研究開発法人理化学研究所 | 薬物動態関連遺伝子の網羅的配列解析法とそれに使用されるプライマーセット |
| US11396674B2 (en) * | 2019-02-22 | 2022-07-26 | Gianluca ROMA | Multiplex nucleic acid amplification and library preparation |
| US10941453B1 (en) | 2020-05-20 | 2021-03-09 | Paragon Genomics, Inc. | High throughput detection of pathogen RNA in clinical specimens |
| CN114875119B (zh) * | 2021-02-05 | 2025-11-18 | 广东菲鹏生物有限公司 | 一种提高核酸扩增产物浓度的方法及其应用 |
| CN114369649A (zh) * | 2022-02-08 | 2022-04-19 | 山东见微生物科技有限公司 | 一种特异的选择扩增及多重pcr方法和应用 |
| US12091715B2 (en) | 2022-04-21 | 2024-09-17 | Paragon Genomics, Inc. | Methods and compositions for reducing base errors of massive parallel sequencing using triseq sequencing |
| US11680293B1 (en) | 2022-04-21 | 2023-06-20 | Paragon Genomics, Inc. | Methods and compositions for amplifying DNA and generating DNA sequencing results from target-enriched DNA molecules |
| CN114774526B (zh) * | 2022-04-25 | 2025-12-12 | 陕西科技大学 | 一种dna长度的熔解测定方法及试剂盒 |
Family Cites Families (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5750341A (en) | 1995-04-17 | 1998-05-12 | Lynx Therapeutics, Inc. | DNA sequencing by parallel oligonucleotide extensions |
| US20040002156A1 (en) | 2002-06-26 | 2004-01-01 | Stratagene | Selective cloning of homoduplex nucleic acids |
| AU2004209426B2 (en) * | 2003-01-29 | 2008-04-17 | 454 Life Sciences Corporation | Method for preparing single-stranded DNA libraries |
| EP1574570A1 (en) * | 2004-03-12 | 2005-09-14 | Universität Regensburg | Process for reducing the number of mismatches in double stranded polynucleotides |
| WO2006023919A2 (en) * | 2004-08-24 | 2006-03-02 | Cornell Research Foundation, Inc. | Detection of nucleic acid differences using endonuclease cleavage/ligas releasing reactions and capillary electrophoresis or microarrays |
| US20060281113A1 (en) | 2005-05-18 | 2006-12-14 | George Church | Accessible polynucleotide libraries and methods of use thereof |
| US8716190B2 (en) | 2007-09-14 | 2014-05-06 | Affymetrix, Inc. | Amplification and analysis of selected targets on solid supports |
| US9540637B2 (en) | 2008-01-09 | 2017-01-10 | Life Technologies Corporation | Nucleic acid adaptors and uses thereof |
| US8586310B2 (en) | 2008-09-05 | 2013-11-19 | Washington University | Method for multiplexed nucleic acid patch polymerase chain reaction |
| US9957558B2 (en) | 2011-04-28 | 2018-05-01 | Life Technologies Corporation | Methods and compositions for multiplex PCR |
| EP2943590A4 (en) | 2013-01-13 | 2017-02-15 | Unitaq Bio | Methods and compositions for pcr using blocked and universal primers |
| WO2015063154A1 (en) | 2013-10-31 | 2015-05-07 | Multiplicom N.V. | Blocking primers in multiplex pcr based assays |
-
2016
- 2016-02-11 CN CN201680021382.3A patent/CN107532166A/zh active Pending
- 2016-02-11 ES ES16749846T patent/ES2795404T3/es active Active
- 2016-02-11 CN CN202510844889.XA patent/CN120700119A/zh active Pending
- 2016-02-11 JP JP2017560886A patent/JP6755890B2/ja active Active
- 2016-02-11 BR BR112017017142-2A patent/BR112017017142B1/pt active IP Right Grant
- 2016-02-11 CN CN202510844827.9A patent/CN120700118A/zh active Pending
- 2016-02-11 WO PCT/US2016/017454 patent/WO2016130746A1/en not_active Ceased
- 2016-02-11 KR KR1020177025384A patent/KR20170110721A/ko not_active Ceased
- 2016-02-11 US US15/041,644 patent/US9464318B2/en active Active
- 2016-02-11 CA CA2976251A patent/CA2976251C/en active Active
- 2016-02-11 AU AU2016219288A patent/AU2016219288B2/en active Active
- 2016-02-11 EP EP16749846.8A patent/EP3256584B1/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN107532166A (zh) | 2018-01-02 |
| CN120700119A (zh) | 2025-09-26 |
| EP3256584B1 (en) | 2020-03-25 |
| US20160230222A1 (en) | 2016-08-11 |
| EP3256584A4 (en) | 2018-08-15 |
| JP2018506307A (ja) | 2018-03-08 |
| EP3256584A1 (en) | 2017-12-20 |
| US9464318B2 (en) | 2016-10-11 |
| BR112017017142B1 (pt) | 2024-03-12 |
| KR20170110721A (ko) | 2017-10-11 |
| ES2795404T3 (es) | 2020-11-23 |
| AU2016219288B2 (en) | 2021-10-28 |
| BR112017017142A2 (pt) | 2018-04-03 |
| JP6755890B2 (ja) | 2020-09-16 |
| CA2976251A1 (en) | 2016-08-18 |
| CN120700118A (zh) | 2025-09-26 |
| AU2016219288A1 (en) | 2017-08-17 |
| WO2016130746A1 (en) | 2016-08-18 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CA2976251C (en) | Methods and compositions for reducing non-specific amplification products | |
| US10421993B2 (en) | Methods and compositions for reducing non-specific amplification products | |
| JP6986509B2 (ja) | 核酸ガイド化ヌクレアーゼをベースとした系を使用する核酸の捕捉 | |
| US20220372548A1 (en) | Vitro isolation and enrichment of nucleic acids using site-specific nucleases | |
| EP1255871B1 (en) | Multiplex ligatable probe amplification | |
| JP6995751B2 (ja) | ガイド核酸を作製および使用するための方法および組成物 | |
| US20190218611A1 (en) | Methods for variant detection | |
| EP2050819A1 (en) | Method for amplification of nucleotide sequence | |
| JP2022511255A (ja) | ライブラリー富化を改善するための組成物および方法 | |
| EP3330386A1 (en) | Preparation of adapter-ligated amplicons | |
| US20210388414A1 (en) | Optimization of in vitro isolation of nucleic acids using site-specific nucleases | |
| US20210395799A1 (en) | Methods for variant detection | |
| HK1240612B (en) | Method and compositions for reducing non-specific amplification products | |
| EP4296372A1 (en) | Method to detect and discriminate cytosine modifications | |
| EP3610030B1 (en) | Methods for variant detection |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request |
Effective date: 20201118 |
|
| EEER | Examination request |
Effective date: 20201118 |
|
| EEER | Examination request |
Effective date: 20201118 |
|
| EEER | Examination request |
Effective date: 20201118 |
|
| EEER | Examination request |
Effective date: 20201118 |
|
| EEER | Examination request |
Effective date: 20201118 |