CN107512959A - A kind of bletilla tissue culture seedlings domestication special fertilizer for preventing and treating underground pests and its preparation method and application - Google Patents
A kind of bletilla tissue culture seedlings domestication special fertilizer for preventing and treating underground pests and its preparation method and application Download PDFInfo
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- CN107512959A CN107512959A CN201710914893.4A CN201710914893A CN107512959A CN 107512959 A CN107512959 A CN 107512959A CN 201710914893 A CN201710914893 A CN 201710914893A CN 107512959 A CN107512959 A CN 107512959A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C21/00—Methods of fertilising, sowing or planting
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F3/00—Fertilisers from human or animal excrements, e.g. manure
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/60—Biocides or preservatives, e.g. disinfectants, pesticides or herbicides; Pest repellants or attractants
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- Life Sciences & Earth Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Soil Sciences (AREA)
- Plant Pathology (AREA)
- Environmental Sciences (AREA)
- Fertilizers (AREA)
Abstract
The invention discloses a kind of bletilla tissue culture seedlings domestication special fertilizer for preventing and treating underground pests and its preparation method and application, the special fertilizer is mainly made up of the raw material of following weight ratio：10~20 parts of kuh-seng, 20~30 parts of chinaberry, 5~10 parts of humic acid potassium, 15~20 parts of Oil-tea-cake, 1~5 part of plant growth regulator, 80~120 parts of sheep manure, 100~150 parts of silkworm excrement, 5~10 parts of biofermentation bacterium.The present invention in the organic matters such as sheep manure, silkworm excrement, plant growth regulator and growth-promoting agent by adding the strong vegetable source pesticide material of insect pest ability, bletilla tissue culture seedlings can be promoted quickly to grow, shorten the domestication time, and can effectively prevent and treat the harm of subterranean pest-insect during bletilla tissue culture seedlings domestication.Base manure and vegetable source pesticide component are used in mixed way, play fertile medicine double effects, this method source is wide, and manufacturing cost is low, and preparation method is simple.In being tamed for bletilla tissue culture seedlings, the planting percent of bletilla tissue culture seedlings domestication is drastically increased, and has prevented subterranean pest-insect substantially and has gnawed the phenomenon that bletilla striata rhizome causes insect pest.
Description
Technical field
The present invention relates to a kind of bletilla tissue culture seedlings domestication special fertilizer for preventing and treating underground pests and its preparation method and application, category
In bletilla striata technical field of fertilizers.
Background technology
The bletilla striata is orchid family bletilla striata platymiscium, is used as medicine with stem tuber, has tonifying lung hemostasis, detumescence and promoting granulation and other effects.The bletilla striata is for I
One of rare Chinese medicine of state's folk tradition, the huge market demand.Artificial excessively excavation and Natural habitat make wild white by destruction
Splendid achnatherum resource is drastically reduced, endangered.In order to protect wild resource, meeting the market demand, the artificial growth of the bletilla striata gradually rises,
And for the cultivating and growing of the bletilla striata mainly based on division propagation, its breeding coefficient is low, of poor benefits at present.In recent years, with bletilla striata group
The maturation of culture technique is knitted, efficiently plantation gradually forms the bletilla striata of scale, and bletilla tissue culture seedlings need to pass through and tame and docile before transplanting is planted
Change could carry out field production, but bletilla tissue culture seedlings are taming link, due to the manurial-philous characteristic of the bletilla striata, need to periodically carry out top dressing hole
Reason, under conditions of loose matrix, high humidity, high fertile is tamed, subterranean pest-insect is easily grown, and be not easy to discover, cause bletilla striata domesticated seedlings
Rhizome and young shoot are gnawed, and so as to cause bletilla striata seedling dead, are caused damage.
Bletilla striata insect pest is mainly subterranean pest-insect, and subterranean pest-insect is the formidable enemy of the bletilla striata, eats evil pseudobulb, young shoot, rhizome, causes
It is short of seedling dead seedling, subterranean pest-insect mainly has mole cricket (mqb), grub (chafer), wireworm and cutworm etc., due to these ground
The under ground portion of the lower pest damage bletilla striata, hides by day and comes out at night, and causes harm in nearly Tu Mianchu, is not easy to be noticeable, and preventing and treating is relatively difficult.
The base manure used of bletilla tissue culture seedlings domestication at present, mainly based on spacetabs type composite fertilizer, Fertilizer nutrient is not comprehensive, and
The effect of without insect-pest, integrated application effect is undesirable, and be still greatly improved space.
The content of the invention
Goal of the invention：For above-mentioned technical problem, the invention provides a kind of bletilla tissue culture seedlings for preventing and treating underground pests to tame and docile
Change special fertilizer and its preparation method and application.
Technical scheme：To achieve the above object of the invention, the present invention provides a kind of bletilla tissue culture seedlings for preventing and treating underground pests and tamed and dociled
Change special fertilizer, it is mainly made up of the raw material of following weight ratio：
10~20 parts of kuh-seng, 20~30 parts of chinaberry, 5~10 parts of humic acid potassium, 15~20 parts of Oil-tea-cake,
1~5 part of plant growth regulator, 80~120 parts of sheep manure, 100~150 parts of silkworm excrement, 5~10 parts of biofermentation bacterium.
It is preferred that it is mainly made up of the raw material of following weight ratio：
10~20 parts of kuh-seng, 22~28 parts of chinaberry, 6~8 parts of humic acid potassium, 16~18 parts of Oil-tea-cake,
2~4 parts of plant growth regulator, 90~110 parts of sheep manure, 110~140 parts of silkworm excrement, 6~8 parts of biofermentation bacterium.
The main component of the plant growth regulator is methyl α-naphthyl acetate, and its content is 1~3%.
The biofermentation bacterium is ferment bacterium quick rotting agent (cfu >=0.5 hundred million) and phosphorus decomposing bacillus megaterium bacterium powder (>=100
Hundred million/gram) in mass ratio 1:3 mixed preparings form.
The preparation method of the bletilla tissue culture seedlings domestication special fertilizer of preventing and treating underground pests of the present invention, comprises the following steps：
(1) each raw material is weighed in proportion, kuh-seng, chinaberry, Oil-tea-cake drying is then comminuted into powdered.
(2) biofermentation bacterium is put into 30~40 DEG C of water and dissolved, zymocyte liquid is made, then by above-mentioned kuh-seng, hardship
Zymocyte liquid is spilled into after chinaberry and Oil-tea-cake powder and humic acid potassium are well mixed, heap is carried out and vomits fermentation 10~20 days, fermentation
During, it is less than 60 DEG C to keep temperature in heap, and turning cooling is carried out when temperature is more than 60 DEG C；
(3) after kuh-seng, chinaberry, Oil-tea-cake are decomposed, addition sheep manure, silkworm excrement and plant growth regulator, second is carried out
Secondary heap is vomitted fermentation, ferments 30~60 days；In fermentation process, it is less than 60 DEG C to keep temperature in heap, is carried out when temperature is more than 60 DEG C
Turning cools, and after fermentation fully, produces the fertilizer.
The bletilla tissue culture seedlings domestication special fertilizer of preventing and treating underground pests of the present invention, can prevent and treat bletilla striata field underground pests,
And bletilla striata growth can also be effectively facilitated.
Beneficial effect：Compared with prior art, technical solution of the present invention has the advantage that：
(1) present invention is in fertilizer by adding with the strong plant source of killing ability, by heap vomit fermentation be made it is organic
Fertilizer, the insect pest during bletilla tissue culture seedlings domestication can be effectively prevented and treated, avoided because using the preventing and treating such as chemical pesticide phoxim, chlopyrifos
Subterranean pest-insect and cause soil to be polluted；
(2) the plant growth regulator main component added in the present invention is methyl α-naphthyl acetate, is had to bletilla tissue culture seedlings domestication
Stratification and the effect taken root, be hybridly prepared into other components survival rate that bletilla striata seedling can be greatly improved after organic fertilizer and
Germination percentage；
(3) raw material sources of the present invention are wide, cheap, and preparation technology is simple, tamed in bletilla tissue culture seedlings
During, applied as base manure, cost is low, and effect is good, can effectively control bletilla striata insect pest, promotes bletilla striata germination and growth, should
With having a extensive future.
Embodiment
According to following embodiments, the present invention may be better understood.It is however, as it will be easily appreciated by one skilled in the art that real
Apply the content described by example and be merely to illustrate the present invention, without should be also without limitation on sheet described in detail in claims
Invention.
Biofermentation bacterium is ferment bacterium quick rotting agent (cfu >=0.5 hundred million) and phosphorus decomposing bacillus megaterium in following examples
Bacterium powder (>=100 hundred million/gram) in mass ratio 1:3 mixed preparings form.
Embodiment 1
Prescription：
20 parts of kuh-seng, 20 parts of chinaberry, 10 parts of humic acid potassium, 20 parts of Oil-tea-cake, plant growth regulator (methyl α-naphthyl acetate content
For 2%) 5 parts, 120 parts of sheep manure, 150 parts of silkworm excrement, 10 parts of biofermentation bacterium.
Preparation method：
(1) above raw material is weighed in parts by weight：
(2) by kuh-seng, chinaberry, Oil-tea-cake drying and then crushing.
(3) biofermentation bacterium is put into 30~40 DEG C of water and dissolved, zymocyte liquid is made, then by kuh-seng, chinaberry,
Zymocyte liquid is spilled into after Oil-tea-cake and humic acid potassium are well mixed, progress heap, which is vomitted, to ferment 15 days, in fermentation process, keeps temperature in heap
Spend for less than 60 DEG C, turning cooling is carried out when temperature is more than 60 DEG C.
(4) after the kuh-seng in (3), chinaberry, Oil-tea-cake are decomposed, the sheep manure of addition (1) described component, silkworm excrement, plant
Growth regulator, carry out second of heap and vomit fermentation, fermentation 45 days.In fermentation process, it is less than 60 DEG C to keep temperature in heap, temperature
Turning cooling is carried out during more than 60 DEG C.After fermentation fully, the fertilizer is produced.
Embodiment 2
Prescription：
20 parts of kuh-seng, 20 parts of chinaberry, 10 parts of humic acid potassium, 15 parts of Oil-tea-cake, plant growth regulator (methyl α-naphthyl acetate content
For 2%) 8 parts, 100 parts of sheep manure, 120 parts of silkworm excrement, 8 parts of biofermentation bacterium.
Preparation method：
(1) above raw material is weighed in parts by weight：
(2) by kuh-seng, chinaberry, Oil-tea-cake drying and then crushing.
(3) biofermentation bacterium is put into 30~40 DEG C of water and dissolved, zymocyte liquid is made, then by kuh-seng, chinaberry,
Zymocyte liquid is spilled into after Oil-tea-cake and humic acid potassium are well mixed, progress heap, which is vomitted, to ferment 15 days, in fermentation process, keeps temperature in heap
Spend for less than 60 DEG C, turning cooling is carried out when temperature is more than 60 DEG C.
(4) after the kuh-seng in (3), chinaberry, Oil-tea-cake are decomposed, the sheep manure of addition (1) described component, silkworm excrement, plant
Growth regulator, carry out second of heap and vomit fermentation, fermentation 45 days.In fermentation process, it is less than 60 DEG C to keep temperature in heap, temperature
Turning cooling is carried out during more than 60 DEG C.After fermentation fully, the fertilizer is produced.
Embodiment 3
Prescription：
10 parts of kuh-seng, 30 parts of chinaberry, 10 parts of humic acid potassium, 20 parts of Oil-tea-cake, plant growth regulator (methyl α-naphthyl acetate content
For 1%) 5 parts, 120 parts of sheep manure, 150 parts of silkworm excrement, 10 parts of biofermentation bacterium.
Preparation method：
(1) above raw material is weighed in parts by weight：
(2) by kuh-seng, chinaberry, Oil-tea-cake drying and then crushing.
(3) biofermentation bacterium is put into 30~40 DEG C of water and dissolved, zymocyte liquid is made, then by kuh-seng, chinaberry,
Zymocyte liquid is spilled into after Oil-tea-cake and humic acid potassium are well mixed, progress heap, which is vomitted, to ferment 10 days, in fermentation process, keeps temperature in heap
Spend for less than 60 DEG C, turning cooling is carried out when temperature is more than 60 DEG C.
(4) after the kuh-seng in (3), chinaberry, Oil-tea-cake are decomposed, the sheep manure of addition (1) described component, silkworm excrement, plant
Growth regulator, carry out second of heap and vomit fermentation, fermentation 60 days.In fermentation process, it is less than 60 DEG C to keep temperature in heap, temperature
Turning cooling is carried out during more than 60 DEG C.After fermentation fully, the fertilizer is produced.
Embodiment 4
Prescription：
20 parts of kuh-seng, 20 parts of chinaberry, 5 parts of humic acid potassium, 15 parts of Oil-tea-cake, plant growth regulator (methyl α-naphthyl acetate content
For 3%) 1 part, 80 parts of sheep manure, 100 parts of silkworm excrement, 5 parts of biofermentation bacterium.
Preparation method：
(1) above raw material is weighed in parts by weight：
(2) by kuh-seng, chinaberry, Oil-tea-cake drying and then crushing.
(3) biofermentation bacterium is put into 30~40 DEG C of water and dissolved, zymocyte liquid is made, then by kuh-seng, chinaberry,
Zymocyte liquid is spilled into after Oil-tea-cake and humic acid potassium are well mixed, progress heap, which is vomitted, to ferment 20 days, in fermentation process, keeps temperature in heap
Spend for less than 60 DEG C, turning cooling is carried out when temperature is more than 60 DEG C.
(4) after the kuh-seng in (3), chinaberry, Oil-tea-cake are decomposed, the sheep manure of addition (1) described component, silkworm excrement, plant
Growth regulator, carry out second of heap and vomit fermentation, fermentation 30 days.In fermentation process, it is less than 60 DEG C to keep temperature in heap, temperature
Turning cooling is carried out during more than 60 DEG C.After fermentation fully, the fertilizer is produced.
Embodiment 5
Prescription：
15 parts of kuh-seng, 25 parts of chinaberry, 7.5 parts of humic acid potassium, 17.5 parts of Oil-tea-cake, plant growth regulator (methyl α-naphthyl acetate
Content is 2%) 3 parts, 100 parts of sheep manure, 125 parts of silkworm excrement, 7.5 parts of biofermentation bacterium.
Preparation method：
(1) above raw material is weighed in parts by weight：
(2) by kuh-seng, chinaberry, Oil-tea-cake drying and then crushing.
(3) biofermentation bacterium is put into 30~40 DEG C of water and dissolved, zymocyte liquid is made, then by kuh-seng, chinaberry,
Zymocyte liquid is spilled into after Oil-tea-cake and humic acid potassium are well mixed, progress heap, which is vomitted, to ferment 12 days, in fermentation process, keeps temperature in heap
Spend for less than 60 DEG C, turning cooling is carried out when temperature is more than 60 DEG C.
(4) after the kuh-seng in (3), chinaberry, Oil-tea-cake are decomposed, the sheep manure of addition (1) described component, silkworm excrement, plant
Growth regulator, carry out second of heap and vomit fermentation, fermentation 40 days.In fermentation process, it is less than 60 DEG C to keep temperature in heap, temperature
Turning cooling is carried out during more than 60 DEG C.After fermentation fully, the fertilizer is produced.
Experimental example field control effectiveness test
It is embodiment 1 and the formula described in embodiment 2 with the fertilizer being tested, specific test procedure is as follows：
The bletilla striata domestication place that selection does not carry out soil-borne disease pest control all the year round is tested, site preparation before experiment, ridging,
The bletilla striata dedicated substrate after special 10cm is laid on ridge, and the fertilizer of above-described embodiment is admixed in matrix as base manure, is tried
Test three processing of setting：Example 1, example 2 and blank control；Each processing is repeated 3 times, and each processing area is 20 ㎡, each
Processing 500 plants of bletilla tissue culture seedlings of plantation.After plantation bletilla tissue culture seedlings after 40 days, investigate each processing block subterranean pest-insect and endanger feelings
Condition, every bletilla striata seedling for being bitten or being killed by subterranean pest-insects such as mole cricket, grub, cutworm or wireworms is insect bite seedling, is calculated
Prevention effect simultaneously counts sprouting the mean tillering number.
Percentage of injury (%)=bitten or killed strain tree/total seedling number * 100
Prevention effect (%)=(check plot percentage of injury-treatment region percentage of injury)/(check plot percentage of injury) * 100
The main multipoint random of statistics of sprouting the mean tillering number extracts 50 plants, counts every plant of tissue-cultured seedling mean germination number.
It is all raw materials one time fermentation simultaneously according to the method for the embodiment of the present invention 1 to compare 1 group, fermentation time 15 days；
Compare 2 groups with to compare 1 group essentially identical, difference is that fermentation time is 45 days.
The test method for compareing 1 group and 2 groups of control is same as mentioned above.
Concrete outcome is as follows：
Field control effectiveness test investigation result
It was found from from upper table, in the present invention, embodiment 1 and embodiment 2 are applied to during bletilla tissue culture seedlings domestication, underground
Insect pest is greatly decreased, and preventive effect reaches more than 85.8%, and tissue-cultured seedling sprouting tiller number relative comparison is obviously improved, examination
Test and show, the present invention has the effect of good stratification and control insect pest.
In addition, 1 group and 2 groups of control is according to the inventive method, one time fermentation is only carried out, is whether sent out according to first time
The ferment time, the preventive effect and rush bletilla striata growth result of its gained fertilizer were not ideal enough, aobvious also according to second of fermentation time
Work is separated less than raw material of the present invention and the effect of fermenting twice processing gained fertilizer.
Claims (7)
1. a kind of bletilla tissue culture seedlings domestication special fertilizer for preventing and treating underground pests, it is characterised in that it is mainly by following weight part ratio
The raw material of example is made：
10~20 parts of kuh-seng, 20~30 parts of chinaberry, 5~10 parts of humic acid potassium, 15~20 parts of Oil-tea-cake,
1~5 part of plant growth regulator, 80~120 parts of sheep manure, 100~150 parts of silkworm excrement, 5~10 parts of biofermentation bacterium.
2. the bletilla tissue culture seedlings domestication special fertilizer of preventing and treating underground pests according to claim 1, it is characterised in that its is main
It is made up of the raw material of following weight ratio：
10~20 parts of kuh-seng, 22~28 parts of chinaberry, 6~8 parts of humic acid potassium, 16~18 parts of Oil-tea-cake,
2~4 parts of plant growth regulator, 90~110 parts of sheep manure, 110~140 parts of silkworm excrement, 6~8 parts of biofermentation bacterium.
3. the bletilla tissue culture seedlings domestication special fertilizer of preventing and treating underground pests according to claim 1, it is characterised in that the plant
The main component of thing growth regulator is methyl α-naphthyl acetate, and its content is 1~3%.
4. the bletilla tissue culture seedlings domestication special fertilizer of preventing and treating underground pests according to claim 1, it is characterised in that the life
Thing zymophyte be ferment bacterium quick rotting agent (cfu >=0.5 hundred million) and phosphorus decomposing bacillus megaterium bacterium powder (>=100 hundred million/gram) in mass ratio
1:3 mixed preparings form.
5. the preparation method of the bletilla tissue culture seedlings domestication special fertilizer of any one of the claim 1-4 preventing and treating underground pests, it is special
Sign is, comprises the following steps：
(1) each raw material is weighed in proportion, kuh-seng, chinaberry, Oil-tea-cake drying is then comminuted into powdered.
(2) biofermentation bacterium is put into 30~40 DEG C of water and dissolved, zymocyte liquid is made, then by above-mentioned kuh-seng, chinaberry
With Oil-tea-cake powder and humic acid potassium it is well mixed after be spilled into zymocyte liquid, carry out heap and vomit fermentation 10~20 days, fermentation process
In, it is less than 60 DEG C to keep temperature in heap, and turning cooling is carried out when temperature is more than 60 DEG C；
(3) after kuh-seng, chinaberry, Oil-tea-cake are decomposed, addition sheep manure, silkworm excrement and plant growth regulator, second of heap is carried out
Vomit fermentation, ferment 30~60 days；In fermentation process, it is less than 60 DEG C to keep temperature in heap, and turning is carried out when temperature is more than 60 DEG C
Cooling, after fermentation fully, produce the fertilizer.
6. the bletilla tissue culture seedlings domestication special fertilizer of any one of the claim 1-4 preventing and treating underground pests is under preventing and treating bletilla striata field
Application in insect pest.
7. the bletilla tissue culture seedlings domestication special fertilizer of any one of the claim 1-4 preventing and treating underground pests is in bletilla striata growth is promoted
Application.
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| CN108530234A (en) * | 2018-07-20 | 2018-09-14 | 贵州珍安药业有限公司 | A kind of bletilla striata plantation special fertilizer and preparation method thereof |
| CN108558554A (en) * | 2018-04-10 | 2018-09-21 | 霍山县顺达生态家庭农场 | A kind of sheep manure fermentation substrate for bletilla striata nursery |
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