CN107466972B - Breeding method for protecting tetranychus cinnabarinus - Google Patents
Breeding method for protecting tetranychus cinnabarinus Download PDFInfo
- Publication number
- CN107466972B CN107466972B CN201710845595.4A CN201710845595A CN107466972B CN 107466972 B CN107466972 B CN 107466972B CN 201710845595 A CN201710845595 A CN 201710845595A CN 107466972 B CN107466972 B CN 107466972B
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- CN
- China
- Prior art keywords
- box
- sponge
- leaves
- tetranychus cinnabarinus
- cotton
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 241000344246 Tetranychus cinnabarinus Species 0.000 title claims abstract description 29
- 238000009395 breeding Methods 0.000 title abstract description 6
- 229920000742 Cotton Polymers 0.000 claims abstract description 24
- 239000002985 plastic film Substances 0.000 claims abstract description 14
- 229920006255 plastic film Polymers 0.000 claims abstract description 14
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 13
- 244000195896 dadap Species 0.000 claims abstract description 12
- 238000000034 method Methods 0.000 claims abstract description 8
- 238000003825 pressing Methods 0.000 claims abstract description 5
- 244000070010 Erythrina variegata Species 0.000 claims description 2
- 241000561734 Celosia cristata Species 0.000 abstract description 6
- 210000001520 comb Anatomy 0.000 abstract description 6
- 230000001488 breeding effect Effects 0.000 abstract description 4
- 230000000694 effects Effects 0.000 abstract description 3
- 241000196324 Embryophyta Species 0.000 description 4
- 206010059866 Drug resistance Diseases 0.000 description 2
- 244000046052 Phaseolus vulgaris Species 0.000 description 2
- 241000239290 Araneae Species 0.000 description 1
- 241000238421 Arthropoda Species 0.000 description 1
- 241000219146 Gossypium Species 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- 240000003183 Manihot esculenta Species 0.000 description 1
- 235000016735 Manihot esculenta subsp esculenta Nutrition 0.000 description 1
- 241000218213 Morus <angiosperm> Species 0.000 description 1
- 235000008708 Morus alba Nutrition 0.000 description 1
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 1
- 241001454295 Tetranychidae Species 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 238000012271 agricultural production Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 230000035558 fertility Effects 0.000 description 1
- 239000002917 insecticide Substances 0.000 description 1
- 235000021332 kidney beans Nutrition 0.000 description 1
- 239000000575 pesticide Substances 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Cultivation Of Plants (AREA)
Abstract
A method for breeding Tetranychus cinnabarinus with good seed-keeping effect comprises providing a box with a box cover, filling a layer of sponge with a section slightly smaller than that of the box body at the bottom of the box, spreading a layer of black plastic film with an area slightly smaller than that of the sponge on the sponge, adding appropriate amount of water, and enclosing the wetted cotton sliver around the black plastic film, wherein half of the cotton sliver is on the plastic film and half is on the sponge; wiping the cleaned fresh erythrina cockscomb leaves, wrapping the leaf stalks with wet cotton, enabling the back surfaces of the leaves to face upwards, pressing the leaf stalks with cotton strips, placing a plurality of fresh erythrina cockscomb leaves, picking tetranychus cinnabarinus to the leaves, reversely covering a box cover on the box, and staggering a gap in the length direction of the box; feeding conditions are as follows: the temperature is 25-27 ℃, the relative humidity is 60-70%, the photoperiod is 14L:10D, and the fresh leaves are replaced every two days. The method can effectively control the size of the tetranychus cinnabarinus population, and can preserve the population for a long time so as to culture tetranychus cinnabarinus sensitive or high-resistance strains.
Description
Technical Field
The invention belongs to the technical field of mite feeding, and relates to a breed conservation feeding mode of tetranychus cinnabarinus.
Background
Tetranychus cinnabarinus is a phytophagous harmful mite, has a wide host range, causes serious economic loss by injuring various economic crops and ornamental plants such as flowers, beans, cotton, vegetables, mulberry trees, fruit trees and the like, and is a worldwide economic harmful mite. In the practice process of agricultural production, people use a large amount of various insecticides to control tetranychus cinnabarinus, but because of the characteristics of tetranychus cinnabarinus, such as very strong fecundity, serious generation overlapping, short growth cycle and the like, the tetranychus cinnabarinus generates different degrees of drug resistance to various medicaments, and is considered to be one of the most serious arthropods in global drug resistance at present. In order to find a novel environment-friendly pesticide for preventing and controlling tetranychus cinnabarinus and effectively controlling the harm of tetranychus cinnabarinus, a large number of standard insect sources must be stably provided so as to ensure that experts and scholars in the field of pest and disease science and plant protection develop a series of research needs on tetranychus cinnabarinus.
At present, tetranychus cinnabarinus is bred on cassava, cotton, kidney beans and other plants, but the breeding on the plants cannot keep the population density higher, and when tetranychus cinnabarinus is needed to be used for relevant research, a large amount of manpower and time are needed for selection, and the effect is poor.
Disclosure of Invention
The invention aims to provide a breed conservation feeding method of tetranychus cinnabarinus, and aims to solve the problems that the operation is inconvenient and the effect is poor when tetranychus cinnabarinus is artificially fed and separated in the prior art.
The invention is realized by the following technical scheme.
The invention relates to a breed conservation and feeding method of tetranychus cinnabarinus, which adopts erythrina cristata to feed tetranychus cinnabarinus on a water-borne feeding table and comprises the following specific steps.
(1) Preparation of the aquatic breeding platform: taking a box with a box cover, padding a layer of sponge with the thickness of 2-2.5 cm and the section slightly smaller than the box body at the bottom in the box, laying a layer of black plastic film with the area slightly smaller than the sponge on the sponge, adding a proper amount of water, wherein the height of the water surface is 0.5cm lower than that of the sponge, surrounding the black plastic film with a cotton sliver wetted by the water, and placing half of the cotton sliver on the plastic film and half of the cotton sliver on the sponge.
(2) Inoculating tetranychus cinnabarinus: wiping the cleaned fresh leaves of the erythrina cristata, wrapping the petioles with wet cotton, enabling the back surfaces of the leaves to face upwards, pressing the petioles with the cotton strips, placing a plurality of fresh leaves of the erythrina cristata in each box, picking the tetranychus cinnabarinus to the leaves, reversely covering the box cover on the box, and staggering a gap in the length direction of the box.
Feeding conditions are as follows: the temperature is 25-27 ℃, the relative humidity is 60-70%, the photoperiod is 14L:10D, and the fresh leaves are replaced every two days.
The box with the box cover is preferably 20-25 cm long, 13-15 cm wide and 4-6 cm high; 6-8 fresh leaves of the erythrina variegata are placed in each box.
The method can effectively control the size of the tetranychus cinnabarinus population, and can preserve the population for a long time so as to culture tetranychus cinnabarinus sensitive or high-resistance strains.
Detailed Description
The invention will be further illustrated by the following examples.
Example 1.
A box with a box cover and a length of 20cm, a width of 13 and a height of 4cm is used, a layer of sponge with a thickness of 2cm and a section slightly smaller than that of the box body is padded at the bottom in the box, a black plastic film with an area slightly smaller than that of the sponge is further paved on the sponge, a proper amount of water is added, the height of the water surface is about 0.5cm lower than that of the sponge, a cotton strip wetted with the water surrounds the periphery of the black plastic film, one half of the cotton strip is arranged on the plastic film.
Wiping the cleaned fresh leaves of the cockscomb erythrina, wrapping the leaf stalks with wet cotton, enabling the back surfaces of the leaves to face upwards, pressing the leaf stalks with the cotton strips, placing 6 fresh leaves of the cockscomb erythrina in each box, picking the tetranychus cinnabarinus to the leaves, reversely covering the box cover on the box, and staggering a gap with the width of 2cm in the length direction of the box.
In a climatic incubator, the set temperature is 25 ℃, the relative humidity is 65%, the photoperiod is 14L:10D, the fresh leaves are replaced every two days, the number of the leaves in the incubator is kept to be 6, and the spider mite population is about 100.
Example 2.
A box with a box cover and a length of 25cm, a width of 15cm and a height of 6cm is used, a layer of sponge with a thickness of 2.5cm and a section slightly smaller than that of the box body is padded at the bottom in the box, a layer of black plastic film with an area slightly smaller than that of the sponge is further paved on the sponge, a proper amount of water is added, the height of the water surface is about 0.5cm lower than that of the sponge, a cotton sliver wetted with the water surrounds the periphery of the black plastic film, one half of the cotton sliver is arranged on the plastic.
Wiping the cleaned fresh leaves of the cockscomb erythrina, wrapping the leaf stalks with wet cotton, enabling the back surfaces of the leaves to face upwards, pressing the leaf stalks with the cotton strips, placing 8 fresh leaves of the cockscomb erythrina in each box, picking the tetranychus cinnabarinus to the leaves, reversely covering the box cover on the box, and staggering a gap with the width of 2cm in the length direction of the box.
In a climatic incubator, the set temperature is 27 ℃, the relative humidity is 70 percent, the photoperiod is 14L:10D, the fresh leaves are replaced every two days, the number of the leaves in the incubator is kept to be 8, the replaced leaves are transferred to a new aquatic breeding platform, the same number of the fresh leaves are added, and the population of the spider mites is about 100.
Claims (2)
1. A method for breed conservation and feeding tetranychus cinnabarinus is characterized by comprising the following steps:
(1) taking a box with a box cover, padding a layer of sponge with the thickness of 2-2.5 cm and the section slightly smaller than the box body at the bottom in the box, laying a layer of black plastic film with the area slightly smaller than the sponge on the sponge, adding a proper amount of water, wherein the height of the water surface is 0.5cm lower than that of the sponge, surrounding the black plastic film with a cotton sliver wetted by the water, wherein one half of the cotton sliver is on the plastic film, and the other half of the cotton sliver is on the sponge;
(2) wiping the cleaned fresh leaves of the erythrina cristata to dry, wrapping the petioles of the erythrina cristata with wet cotton, enabling the back surfaces of the leaves to face upwards, pressing the petioles with the cotton strips, placing a plurality of fresh leaves of the erythrina cristata in each box, picking tetranychus cinnabarinus to the leaves, reversely covering the box cover on the box, and staggering a gap in the length direction of the box;
feeding conditions are as follows: the temperature is 25-27 ℃, the relative humidity is 60-70%, the photoperiod is 14L:10D, and the fresh leaves are replaced every two days.
2. The method for keeping and feeding tetranychus cinnabarinus according to claim 1, wherein the box with a box cover has a size of 20cm to 25cm in length, 13 cm to 15cm in width and 4cm to 6cm in height; 6-8 fresh leaves of the erythrina variegata are placed in each box.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710845595.4A CN107466972B (en) | 2017-09-19 | 2017-09-19 | Breeding method for protecting tetranychus cinnabarinus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710845595.4A CN107466972B (en) | 2017-09-19 | 2017-09-19 | Breeding method for protecting tetranychus cinnabarinus |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107466972A CN107466972A (en) | 2017-12-15 |
| CN107466972B true CN107466972B (en) | 2020-10-23 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710845595.4A Active CN107466972B (en) | 2017-09-19 | 2017-09-19 | Breeding method for protecting tetranychus cinnabarinus |
Country Status (1)
| Country | Link |
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| CN (1) | CN107466972B (en) |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103053477B (en) * | 2012-12-26 | 2014-01-22 | 中国农业科学院郑州果树研究所 | Scale breeding method of Scolothrips takahashii |
| CN103053480B (en) * | 2013-01-25 | 2014-04-16 | 中国农业科学院柑桔研究所 | Method for RNA (ribonucleic acid) interference of panonychus citri mites |
| CN103651270A (en) * | 2013-12-03 | 2014-03-26 | 四川农业大学 | Breeding method of small new neoseiulus californicus |
| CN103858828A (en) * | 2014-03-27 | 2014-06-18 | 李庆 | Method for breeding neoseiulus californicus through apricot blossom pollen |
| CN104472437A (en) * | 2014-11-20 | 2015-04-01 | 中国农业科学院柑桔研究所 | Indoor isolation feeder for spider mites |
| CN204259646U (en) * | 2014-11-20 | 2015-04-15 | 中国农业科学院柑桔研究所 | The device of artificial indoor rearing tetranychid |
| CN204443739U (en) * | 2015-02-04 | 2015-07-08 | 北京市农林科学院 | The anti-escape observation box of a kind of cultivation tetranychid |
| CN205912712U (en) * | 2016-07-13 | 2017-02-01 | 贵州省烟草公司遵义市公司 | Arma custos nymph 1 2 age raise device |
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- 2017-09-19 CN CN201710845595.4A patent/CN107466972B/en active Active
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| Publication number | Publication date |
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| CN107466972A (en) | 2017-12-15 |
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