CN107439189A - The method of the high-quality seedling of sealwort rhizome quickly breeding - Google Patents

The method of the high-quality seedling of sealwort rhizome quickly breeding Download PDF

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CN107439189A
CN107439189A CN201710720760.3A CN201710720760A CN107439189A CN 107439189 A CN107439189 A CN 107439189A CN 201710720760 A CN201710720760 A CN 201710720760A CN 107439189 A CN107439189 A CN 107439189A
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sealwort
rhizome
days
relative humidity
illumination
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罗婵
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Changsha Aiyang Medical Technology Co Ltd
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Changsha Aiyang Medical Technology Co Ltd
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G7/00Botany in general
    • A01G7/06Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01CPLANTING; SOWING; FERTILISING
    • A01C1/00Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01CPLANTING; SOWING; FERTILISING
    • A01C1/00Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
    • A01C1/08Immunising seed
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G22/00Cultivation of specific crops or plants not otherwise provided for

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  • Environmental Sciences (AREA)
  • Soil Sciences (AREA)
  • Botany (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Ecology (AREA)
  • Forests & Forestry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Cultivation Of Plants (AREA)

Abstract

The present invention relates to the method for providing the high-quality seedling of sealwort rhizome quickly breeding, pretreatment, radix polygonati officinalis through sealwort rhizome discard rhizome processing, activation processing, lure bud processing, Heat thermostability, lure the steps such as root processing, small transplantation of seedlings, field management to be obtained.The method is handled by direct wind solarization after excavation, is reduced sealwort rhizome reclaimed water point content, be slow down the corrupt speed of sealwort, while provides condition again for later stage quick pregermination;The usage amount of the high-quality rhizome of sealwort can be reduced, and can promotes the neat and consistent of sealwort sprout, greatly reduces sealwort cultivation management cost.

Description

The method of the high-quality seedling of sealwort rhizome quickly breeding
Technical field
Present invention relates particularly to a kind of method of the high-quality seedling of sealwort rhizome quickly breeding, belongs to medicinal plant cultivation technology neck Domain.
Background technology
Sealwort also known as:Polygonatum sibiricum Redoute, yellow chicken dish, pipe dish, claw ginseng, tendrilleaf solomonseal rhizome, achickenclaw ginseng.Root-like stock is cylindric, by Expanded in tubercle, therefore " internode " is one thick, one thin, and having short branch at thick one, (Chinese medicinal herbal claims this root-like stock type Made medicinal material is polygonatum sibiricum Redoute), 1~2 centimetre of diameter.Stem is high 50~90 centimetres, or is in climb up by holding on to sometimes up to more than 1 meter Shape.Impeller is given birth to, and often takes turns 4~6 pieces, strip lanceolar is long 8~15 centimetres, and 6~16 millimeters of wide (4~), tip fist is rolled up or bent to Hook.Inflorescence generally 2~4 flowers of tool, like into umbrella shape shape, total bennet grows 1~2 centimetre, bennet length 4~10 millimeters of (2.5~), bows Hang down;Bract is located at bennet base portion, film quality, bores shape or strip lanceolar, long 3~5 millimeters, has 1 arteries and veins;Perianth milky is to yellowish Color, 9~12 millimeters of total length, contracting of slightly hanging in the middle part of perianth tube, sliver are about 4 millimeters;Filigree grows 0.5~1 millimeter, the milli of flower pesticide length 2~3 Rice;Ovary is about 3 millimeters, and style grows 5~7 millimeters.7~10 millimeters of berry diameter, black, have 4~7 seeds.Florescence 5~6 Month, 8~September of fruiting period.It is mainly distributed on Heilungkiang, Jilin, Liaoning, Hebei, Shanxi, Shaanxi, the Inner Mongol, Ningxia, Gansu (east Portion), Henan, Shandong, Anhui (east), Zhejiang (northwestward).At raw sylvan life, shrubbery or hillside the moon, height above sea level 800-2800 rice.
Sealwort medicinal effects are the root of liliaceous plant sealwort, P. kingianum, David's-harp and polygonatum filipes.Supported with tonifying Qi The moon, invigorating the spleen, moistening lung, kidney-nourishing effect, the weakness of the spleen and the stomach, fatigue and asthenia for the treatment of, dry deficiency of food, deficiency syndrome of the lung cough caused by dryness, asthenia of essence and blood, interior heat disappear It is thirsty to wait disease.It is very effective in cure for diabetes.Sealwort is called tendrilleaf solomonseal rhizome, chicken head ginseng.The effect of Chinese medicine sealwort and effect.
Sealwort is with root-like stock breeding or seminal propagation at present, but rhizome water content is very high, to during emergence from implantation soil Between it is long, it is perishable.And sealwort seed will typically emerge, it is necessary to by chilling treatment by the 3rd year, and emergence rate is also low Under.
The content of the invention
It is sufficient for water content of sealwort rhizome height itself, content of starch, directly use and cut rhizome segmentation cultivation bud ratio not Height, less than 30% or so, and the sprout cultivated is not healthy and strong, and the present invention provides a kind of high-quality bud of sealwort rhizome quickly breeding Seedling-growing method, the sprout that the method is cultivated have the characteristics that healthy and strong, disease-free, bud ratio is high, reach suitable industrialized production, formula The advantages that effect is good, and bud ratio is high, and incubation time is short, and plant strain growth is vigorous, workable, and application value is high.
The method that the present invention provides the high-quality seedling of sealwort rhizome quickly breeding, it is comprised the following steps that:
(1) pretreatment of sealwort rhizome:The sealwort fresh quench stem of annual excavation in 10~December is selected, goes soil direct after tiling Wind is shone 2~3 days, and discarded rhizome is cleaned with 6~10% carbendazim solutions after the coring of removal sealwort rhizome.
(2) sealwort rhizome is handled:Each sealwort rhizome is cut into the segment rhizome of 2~4 stipes, then with after sterilization After grain active carbon is mixed, relative humidity maintains 40~55%, at 15~20 DEG C, carries out processing 10~25 days.
(3) low-temp activation is handled:The segment rhizome of each sealwort is placed on relative humidity 60~75%, carries out 3~8 DEG C 30~60min of low-temperature treatment.
(4) bud is lured to handle:The segment rhizome of processing is put into and lures 15~30min of immersion, taking-up in bud processing solution to be put into In the sandy soil of sterilization, relative humidity is maintained 60~75%, and intensity of illumination 600~800LX, daily 10~12h of illumination, temperature exist 15~22 DEG C, grow sprout within 25~40 days.
(5) Heat thermostability:The sealwort rhizome to put out new shoots is put and Heat thermostability is carried out at 45~50 DEG C 3~5 seconds, is continued Spray water keeps gauze moistening, maintains relative humidity 50~75%, and temperature is at 15~18 DEG C, again 45 after culture 1~2 day Heat thermostability is carried out at~50 DEG C 3~5 seconds.
(6) root is lured to handle:Band bud rhizome is taken out after Heat thermostability, it is common three times with the processing of root solution is educated, between handling three times It is 3~5 days every the time, is sprayed 10~35kg/ days per 100kg sealworts rhizome, and point 3~5 sprinklings, cultivation temperature 15~22 DEG C, daily illumination 10~13 hours, 1000~2000LX of intensity of illumination, relative humidity control is 60~75%, culture 25~45 My god.
(7) the small transplantation of seedlings of sealwort:Sealwort seedling with a large amount of fibrous roots is taken out, then is transplanted in Nutrition Soil, culture temperature 18~25 DEG C of degree, daily illumination 8~12 hours, 700~1000LX of intensity of illumination, relative humidity are controlled 50~70%.
(8) sealwort field management:Transplanting greenhouse culture to overground part, which pours in separately seedling, to be terminated, then is grafted directly to big Tanaka and is carried out Cultivation, crop field relative humidity are controlled 40~60%;Temperature control enters water-filling at 10~30 DEG C, using conventional sealwort cultural method It is in charge of reason, fertilizer management and the prevention and control of plant diseases, pest control.
Sealwort rhizome is placed in mesh in above-mentioned processing procedure, prevents sealwort rhizome to be immersed in the aqueous solution.
Method for disinfection is after 12% carbendazim solution soaks 12h in above-mentioned steps (2) and (4), and removing carbendazim solution is Can.
In a specific embodiment, the compound method of 7% carbendazim solution described in step (1) is purchased to weigh market The powder of carbendazim 7g bought, the water for adding 93g are mixed.
In a specific embodiment, grain active carbon size described in step (2) is 0.5~5 mesh;Grain active carbon Usage amount kg and sealwort rhizome weight kg ratios be 0.5~6kg:50kg.
In a specific embodiment, bud processing solution is lured to refer to the 6- containing 60~120mg/L described in step (4) BA (6- benzyls aminoadenine), 0.03~0.08g/L GA3The aqueous solution of (gibberellin), 20~50g/L carbendazim.
In a specific embodiment, educated described in step (5) root solution refer to containing 50~100mg/L NAA (how Acetic acid), the aqueous solution of 10~30g/L carbendazim.
In a specific embodiment, Nutrition Soil described in step (7), can make by oneself, and its formula is garden mould 5~10 Part, 3~6 parts of cake-shaped rape seed dregs, 2~3 parts of river sand, 1~2 part of plant ash, mixing.
In a specific embodiment, water management is using drop in conventional sealwort cultural method described in step (8) Pipe mode carries out moisturizing;Fertilizer management is to spill to apply through cake fertilizer, every mu of 250~600kg of application to plantation crop field.
The compound method of above-mentioned cake fertilizer is cake-shaped rape seed dregs and tea oil residule 2~3:1.
In a specific embodiment, the prevention and control of plant diseases, pest control is discovery in conventional sealwort cultural method described in step (8) Sealwort virosis occurs, and in sick cave with 5% bacterium water cleaning agent, or diseased plant is extracted;If it find that chafer is endangered with 90% 800~1000 times of liquid of metrifonate are crystallized, are sprayed 1 time within every 5~7 days, even spray 2~3 times.
Technique effect:
1st, sealwort is handled by direct wind solarization after excavation, is reduced sealwort rhizome reclaimed water point content, be slow down the corruption of sealwort Speed is lost, while provides condition again for later stage quick pregermination.
2nd, the method can reduce the usage amount of the high-quality rhizome of sealwort, and and can promotes the neat and consistent of sealwort sprout, significantly Reduce sealwort cultivation management cost.
3rd, the method obtains sealwort sprout and carries out artificial cultivation, and planting percent is higher than direct heeling-in Propagation of Rhizomes rate, more can be fast Fast reform of nature environment, can shorten the planting rhizoma polygonati time limit, can also improve the content of Siberian solomonseal rhizome polysaccharide.
Embodiment
Below, the present invention will be further detailed with embodiment, but it is not limited to any of these embodiments Individual or similar example.
Embodiment 1
The sealwort fresh quench stem of annual excavation in 10~December is selected, goes directly wind after soil tiling to shine 3 days, removes sealwort rhizome Coring after with 6~10% carbendazim solutions clean discarded rhizome.Each sealwort rhizome is cut into the segment of 2~4 stipes Rhizome 100kg, then with sterilization after 3 mesh grain active carbon 10kg mixed after, relative humidity maintains 50% or so, 18 At DEG C, processing 15 days is carried out.The segment rhizome of each sealwort is placed on relative humidity 65%, carries out 6 DEG C of low-temperature treatments 40min.The segment rhizome of processing is put into 6-BA, 0.04g/L GA containing 70mg/L3, 30g/L carbendazim the aqueous solution in 30min is soaked, taking-up is put into the sandy soil of sterilization, maintains relative humidity 65%, intensity of illumination 700LX, daily illumination 10h, Temperature grows sprout in 30 days at 18 DEG C.The sealwort rhizome to put out new shoots is put and Heat thermostability is carried out at 45 DEG C 5 seconds, continues to spray Watering keeps gauze moistening, maintains relative humidity 55%, and for temperature at 18 DEG C, culture carries out heat shock at 45 DEG C again after 2 days Processing 5 seconds.Band bud rhizome is taken out after Heat thermostability, with 90mg/L NAA, 15g/L the carbendazim aqueous solution (educating root solution) place Altogether three times, it is 3 days to handle interval time three times to reason, and total sprinkling 20kg educates root solution daily, and point 4 sprinklings (are once sprayed 5kg educates root solution), 18 DEG C of cultivation temperature, daily illumination 10 hours, intensity of illumination 1500LX, relative humidity control is 65%, training Support 40 days.Sealwort seedling with a large amount of fibrous roots is taken out, then is transplanted to Nutrition Soil and (is formulated as 10 parts of garden mould, 5 parts of cake-shaped rape seed dregs, river Husky 2 parts, 1 part of plant ash) in, 20 DEG C of cultivation temperature, daily illumination 12 hours, intensity of illumination 800LX, relative humidity, which controls, to exist 50%.Transplanting greenhouse culture to overground part, which pours in separately seedling, to be terminated, then is grafted directly to big Tanaka and is cultivated, crop field relative humidity control System is 46%;Temperature control carries out water management at 20 DEG C, using conventional sealwort cultural method, fertilizer management (uses dropper side Formula carries out moisturizing;Fertilizer management is to spill to apply cake fertilizer (cake-shaped rape seed dregs and tea oil residule 2 to plantation crop field:1), every mu of application 400kg, pest and disease damage During preventing and treating, it is found that sealwort virosis occurs, in sick cave with 5% bacterium water cleaning agent, or diseased plant extracted;If it find that chafer 90% crystallization 800~1000 times of liquid of metrifonate of harm, spray 1 time, even spray 2~3 times for every 5~7 days.Finally obtain 1668 kinds Seedling, it was observed that seedling root system is very flourishing (root length is 14~19cm), plantation collects sealwort rhizome after 3 years, dries, adopts Detection Siberian solomonseal rhizome polysaccharide is carried out with Phenol-sulphate acid method, its content is 11.47%.
Comparative examples 1
The sealwort fresh quench stem of annual excavation in 10~December is selected, goes directly wind after soil tiling to shine 3 days, removes sealwort rhizome Coring after with 6~10% carbendazim solutions clean discarded rhizome.Each sealwort rhizome is cut into the segment of 2~4 stipes Rhizome 100kg, then with sterilization after 3 mesh grain active carbon 10kg mixed after, relative humidity maintains 50% or so, 18 At DEG C, processing 15 days is carried out.The segment rhizome of each sealwort is placed on relative humidity 65%, carries out 6 DEG C of low-temperature treatments 40min.The segment rhizome of processing is put into 6-BA, 0.04g/L GA containing 70mg/L3, 30g/L carbendazim the aqueous solution in 30min is soaked, taking-up is put into the sandy soil of sterilization, maintains relative humidity 65%, intensity of illumination 700LX, daily illumination 10h, Temperature grows sprout in 30 days at 18 DEG C.By sealwort rhizome 90mg/L NAA to put out new shoots, 15g/L the carbendazim aqueous solution Altogether three times, it is 3 days to handle interval time three times for (educating root solution) processing, and total sprinkling 20kg educates root solution, and point 4 sprays daily Spill, 18 DEG C of cultivation temperature, daily illumination 10 hours, intensity of illumination 1500LX, relative humidity control is cultivated 40 days 65%.Will Sealwort seedling with a large amount of fibrous roots takes out, then is transplanted to Nutrition Soil and (is formulated as 10 parts of garden mould, 5 parts of cake-shaped rape seed dregs, 2 parts of river sand, vegetation 1 part of ash) in, 20 DEG C of cultivation temperature, daily illumination 12 hours, intensity of illumination 800LX, relative humidity control is 50%.Transplanting is big Canopy culture to overground part, which pours in separately seedling, to be terminated, then is grafted directly to big Tanaka and is cultivated, and crop field relative humidity is controlled 46%;Temperature Degree control carries out water management at 20 DEG C, using conventional sealwort cultural method, fertilizer management (carries out moisturizing using dropper mode; Fertilizer management is to spill to apply cake fertilizer (cake-shaped rape seed dregs and tea oil residule 2 to plantation crop field:1), every mu of application 400kg, during the prevention and control of plant diseases, pest control, find Sealwort virosis occurs, and in sick cave with 5% bacterium water cleaning agent, or diseased plant is extracted;If it find that chafer is endangered with 90% 800~1000 times of liquid of metrifonate are crystallized, are sprayed 1 time within every 5~7 days, even spray 2~3 times.916 seedlings are finally obtained, it was observed that small Seedling root system is more flourishing (root length is 6~11cm), and plantation collects sealwort rhizome after 3 years, dries, using Phenol-sulphate acid method Detection Siberian solomonseal rhizome polysaccharide is carried out, its content is 7.42%.
Comparative examples 2
The sealwort fresh quench stem of annual excavation in 10~December is selected, goes directly wind after soil tiling to shine 3 days, removes sealwort rhizome Coring after with 6~10% carbendazim solutions clean discarded rhizome.Each sealwort rhizome is cut into the segment of 2~4 stipes Rhizome 100kg, then with sterilization after 3 mesh grain active carbon 10kg mixed after, relative humidity maintains 50% or so, 18 At DEG C, processing 15 days is carried out.The segment rhizome of processing is put into 6-BA, 0.04g/L GA containing 70mg/L3, the more bacterium of 30g/L 30min is soaked in the aqueous solution of spirit, taking-up is put into the sandy soil of sterilization, maintains relative humidity in 65%, intensity of illumination 700LX, Daily illumination 10h, temperature grew sprout in 18 DEG C, 30 days.By sealwort rhizome 9mg/L NAA to put out new shoots, the more bacterium of 15g/L Altogether three times, it is 3 days to handle interval time three times for the aqueous solution (the educating root solution) processing of spirit, sprays 20kg, culture temperature once a day 18 DEG C of degree, daily illumination 10 hours, intensity of illumination 1500LX, relative humidity control are cultivated 40 days 65%.A large amount of palpuses will be carried The sealwort seedling of root takes out, then is transplanted in Nutrition Soil (formula is 10 parts of garden mould, 5 parts of cake-shaped rape seed dregs, 2 parts of river sand, 1 part of plant ash), 20 DEG C of cultivation temperature, daily illumination 12 hours, intensity of illumination 800LX, relative humidity are controlled 50%.Greenhouse culture is transplanted to ground Top pours in separately seedling and terminated, then is grafted directly to big Tanaka and is cultivated, and crop field relative humidity is controlled 46%;Temperature control is 20 DEG C, water management is carried out using conventional sealwort cultural method, fertilizer management (carries out moisturizing using dropper mode;Fertilizer management is Spilt to plantation crop field and apply cake fertilizer (cake-shaped rape seed dregs and tea oil residule 2:1), every mu of application 400kg, during the prevention and control of plant diseases, pest control, sealwort virosis is found Occur, in sick cave with 5% bacterium water cleaning agent, or diseased plant extracted;If it find that 90% crystallization metrifonate of chafer harm 800~1000 times of liquid, spray 1 time within every 5~7 days, even spray 2~3 times.824 seedlings are finally obtained, it was observed that seedling root system is not sent out Up to (root length is 6~10cm), plantation collects sealwort rhizome after 3 years, dries, detection sealwort is carried out using Phenol-sulphate acid method Polysaccharide, its content are 7.15%.
Embodiment 2
The sealwort fresh quench stem of annual excavation in 10~December is selected, goes directly wind after soil tiling to shine 3 days, removes sealwort rhizome Coring after with 6~10% carbendazim solutions clean discarded rhizome.Each sealwort rhizome is cut into the segment of 2~4 stipes Rhizome 100kg, then with sterilization after 5 mesh grain active carbon 25kg mixed after, relative humidity maintains 55%, at 20 DEG C Under, carry out processing 25 days.The segment rhizome of each sealwort is placed on relative humidity 65%, carries out 3 DEG C of low-temperature treatment 60min. The segment rhizome of processing is put into 6-BA, 0.05g/L GA containing 90mg/L3, 50g/L carbendazim the aqueous solution in soak 30min, taking-up are put into the sandy soil of sterilization, maintain relative humidity 70%, intensity of illumination 600LX, daily illumination 12h, temperature At 15 DEG C, sprout is grown within 35 days.The sealwort rhizome to put out new shoots is put and Heat thermostability is carried out at 50 DEG C 3 seconds, continues spray water Gauze moistening is kept, maintains relative humidity 55%, for temperature at 18 DEG C, culture carries out Heat thermostability 3 at 50 DEG C again after 2 days Second.Band bud rhizome is taken out after Heat thermostability, is handled altogether with the aqueous solution (educating root solution) of 80mg/L NAA, 25g/L carbendazim Three times, it is 3~5 days to handle interval time three times, and total sprinkling 20kg educates root solution daily, and point 5 sprinklings (are once sprayed 4kg educates root solution), 22 DEG C of cultivation temperature, daily illumination 13 hours, intensity of illumination 2000LX, relative humidity control is 75%, training Support 35 days.Sealwort seedling with a large amount of fibrous roots is taken out, then is transplanted to Nutrition Soil and (is formulated as 8 parts of garden mould, 6 parts of cake-shaped rape seed dregs, river sand 3 parts, 1 part of plant ash) in, 25 DEG C of cultivation temperature, daily illumination 12 hours, intensity of illumination 1000LX, relative humidity, which controls, to exist 70%.Transplanting greenhouse culture to overground part, which pours in separately seedling, to be terminated, then is grafted directly to big Tanaka and is cultivated, crop field relative humidity control System is 40%;Temperature control carries out water management at 20 DEG C, using conventional sealwort cultural method, fertilizer management (uses dropper side Formula carries out moisturizing;Fertilizer management is to spill to apply cake fertilizer (cake-shaped rape seed dregs and tea oil residule 3 to plantation crop field:1), every mu of application 500kg, pest and disease damage During preventing and treating, it is found that sealwort virosis occurs, in sick cave with 5% bacterium water cleaning agent, or diseased plant extracted;If it find that chafer 90% crystallization 800~1000 times of liquid of metrifonate of harm, spray 1 time, even spray 2~3 times for every 5~7 days.Finally obtain 1754 kinds Seedling, it was observed that seedling root system is very flourishing (root length is 12~18cm), plantation collects sealwort rhizome after 3 years, dries, adopts Detection Siberian solomonseal rhizome polysaccharide is carried out with Phenol-sulphate acid method, its content is 11.63%.

Claims (7)

1. the method for the high-quality seedling of sealwort rhizome quickly breeding, specific steps include as follows:
(1) pretreatment of sealwort rhizome:The sealwort fresh quench stem of annual excavation in 10~December is selected, goes soil direct wind solarization after tiling 2~3 days, remove sealwort rhizome coring after with 6~10% carbendazim solutions clean discard rhizome.
(2) sealwort rhizome is handled:Each sealwort rhizome cuts into the segment rhizome of 2~4 stipes, then with the particle after sterilization After activated carbon is mixed, relative humidity maintains 40~55%, at 15~20 DEG C, carries out processing 10~25 days.
(3) low-temp activation is handled:The segment rhizome of each sealwort is placed on relative humidity 60~75%, carries out 3~8 DEG C of low temperature Handle 30~60min.
(4) bud is lured to handle:The segment rhizome of processing is put into and lures 15~30min of immersion in bud processing solution, taking-up is put into sterilization Sandy soil in, maintain relative humidity 60~75%, intensity of illumination 600~800LX, daily 10~12h of illumination, temperature 15~ 22 DEG C, grow sprout within 25~40 days.
(5) Heat thermostability:The sealwort rhizome to put out new shoots is put and Heat thermostability is carried out at 45~50 DEG C 3~5 seconds, continues to spray Water keeps gauze moistening, maintains relative humidity 50~75%, and temperature is at 15~18 DEG C, again 45~50 after culture 1~2 day Heat thermostability is carried out at DEG C 3~5 seconds.
(6) root is lured to handle:Band bud rhizome is taken out after Heat thermostability, it is common three times with the processing of root solution is educated, three times during processing interval Between be 3~5 days, sprayed 10~35kg/ days per 100kg sealworts rhizomes, and point 3~5 sprinklings, 15~22 DEG C of cultivation temperature, often Its illumination 10~13 hours, 1000~2000LX of intensity of illumination, relative humidity control are cultivated 25~45 days 60~75%.
(7) the small transplantation of seedlings of sealwort:Sealwort seedling with a large amount of fibrous roots is taken out, then is transplanted in Nutrition Soil, cultivation temperature 18 ~25 DEG C, daily illumination 8~12 hours, 700~1000LX of intensity of illumination, relative humidity is controlled 50~70%.
(8) sealwort field management:Transplanting greenhouse culture to overground part, which pours in separately seedling, to be terminated, then is grafted directly to big Tanaka and is planted Training, crop field relative humidity are controlled 40~60%;Temperature control carries out moisture at 10~30 DEG C, using conventional sealwort cultural method Management, fertilizer management and the prevention and control of plant diseases, pest control.
2. method according to claim 1, grain active carbon size described in step (2) is 0.5~5 mesh;Grain active carbon Usage amount kg and sealwort rhizome weight kg ratios are 0.5~6kg:50kg.
3. method according to claim 1, step lures bud processing solution to refer to the 6-BA containing 60~120mg/L described in (4) (6- benzyls aminoadenine), 0.03~0.08g/L GA3The aqueous solution of (gibberellin), 20~50g/L carbendazim.
4. method according to claim 1, educated described in step (5) root solution refer to containing 50~100mg/L NAA (how second Acid), the aqueous solution of 10~30g/L carbendazim.
5. method according to claim 1, Nutrition Soil described in step (7), can make by oneself, it is formulated as 5~10 parts of garden mould, dish Withered 3~6 parts, 2~3 parts of river sand, 1~2 part of plant ash, mixing.
6. method according to claim 1, water management is using dropper side in conventional sealwort cultural method described in step (8) Formula carries out moisturizing;Fertilizer management is to spill to apply through cake fertilizer, every mu of 250~600kg of application to plantation crop field.
7. method according to claim 1, the prevention and control of plant diseases, pest control is discovery sealwort in conventional sealwort cultural method described in step (8) Virosis occurs, and in sick cave with 5% bacterium water cleaning agent, or diseased plant is extracted;If it find that 90% crystallization of chafer harm 800~1000 times of liquid of metrifonate, spray 1 time within every 5~7 days, even spray 2~3 times.
CN201710720760.3A 2017-08-22 2017-08-22 The method of the high-quality seedling of sealwort rhizome quickly breeding Pending CN107439189A (en)

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Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109042116A (en) * 2018-07-27 2018-12-21 安徽千草源生态农业开发有限公司 A kind of implantation methods of high yield rhizoma polygonati
CN109121549A (en) * 2018-08-30 2019-01-04 宣威市龙津生物科技有限责任公司 A method of rhizoma polygonati is improved without bud rhizome germination rate
CN109769613A (en) * 2019-03-27 2019-05-21 四川好土生物科技有限公司 A kind of polygonatum cyrtonema Industrialization of seeds and seedlings mating system
CN115005038A (en) * 2022-07-16 2022-09-06 江西农业大学 Method for rapidly inducing multiple buds of rhizoma polygonati
CN115191305A (en) * 2022-08-02 2022-10-18 江西农业大学 Method for breaking dormancy and rapid leaf growing of polygonatum zingiberensis seeds

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CN105104213A (en) * 2015-10-15 2015-12-02 湖南农业大学 Rapid propagation and seedling culture method using rhizomes of paris polyphylla
CN105145104A (en) * 2015-10-15 2015-12-16 张家界本草科技有限公司 Method for segmenting bletilla striata tubers to perform seedling propagation and raising
CN105165368A (en) * 2015-10-15 2015-12-23 向君民 Method for rapidly breeding seedlings by using bletilla striata tubers

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CN105104213A (en) * 2015-10-15 2015-12-02 湖南农业大学 Rapid propagation and seedling culture method using rhizomes of paris polyphylla
CN105145104A (en) * 2015-10-15 2015-12-16 张家界本草科技有限公司 Method for segmenting bletilla striata tubers to perform seedling propagation and raising
CN105165368A (en) * 2015-10-15 2015-12-23 向君民 Method for rapidly breeding seedlings by using bletilla striata tubers

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109042116A (en) * 2018-07-27 2018-12-21 安徽千草源生态农业开发有限公司 A kind of implantation methods of high yield rhizoma polygonati
CN109121549A (en) * 2018-08-30 2019-01-04 宣威市龙津生物科技有限责任公司 A method of rhizoma polygonati is improved without bud rhizome germination rate
CN109769613A (en) * 2019-03-27 2019-05-21 四川好土生物科技有限公司 A kind of polygonatum cyrtonema Industrialization of seeds and seedlings mating system
CN109769613B (en) * 2019-03-27 2021-07-20 四川好土生物科技有限公司 Polygonatum cyrtonema seedling industrialized breeding method
CN115005038A (en) * 2022-07-16 2022-09-06 江西农业大学 Method for rapidly inducing multiple buds of rhizoma polygonati
CN115191305A (en) * 2022-08-02 2022-10-18 江西农业大学 Method for breaking dormancy and rapid leaf growing of polygonatum zingiberensis seeds
CN115191305B (en) * 2022-08-02 2023-09-15 江西农业大学 Method for breaking dormancy rapid leaf growth of rhizoma polygonati seeds

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