CN107410737A - 一种提高淮猪瘦肉率的专用饲料制备方法 - Google Patents
一种提高淮猪瘦肉率的专用饲料制备方法 Download PDFInfo
- Publication number
- CN107410737A CN107410737A CN201710742868.2A CN201710742868A CN107410737A CN 107410737 A CN107410737 A CN 107410737A CN 201710742868 A CN201710742868 A CN 201710742868A CN 107410737 A CN107410737 A CN 107410737A
- Authority
- CN
- China
- Prior art keywords
- lean meat
- parts
- meat percentage
- improving
- huaihe river
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000002360 preparation method Methods 0.000 title claims abstract description 29
- 235000020997 lean meat Nutrition 0.000 title claims abstract description 28
- 244000061456 Solanum tuberosum Species 0.000 claims abstract description 16
- 235000002595 Solanum tuberosum Nutrition 0.000 claims abstract description 16
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims abstract description 13
- 239000008103 glucose Substances 0.000 claims abstract description 13
- 238000000855 fermentation Methods 0.000 claims abstract description 9
- 230000004151 fermentation Effects 0.000 claims abstract description 9
- 235000015097 nutrients Nutrition 0.000 claims abstract description 8
- 239000004472 Lysine Substances 0.000 claims abstract description 5
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 claims abstract description 5
- 239000000284 extract Substances 0.000 claims abstract description 5
- 241000894006 Bacteria Species 0.000 claims description 18
- 239000000725 suspension Substances 0.000 claims description 12
- 238000002156 mixing Methods 0.000 claims description 10
- 239000012530 fluid Substances 0.000 claims description 8
- 235000010413 sodium alginate Nutrition 0.000 claims description 8
- IXPNQXFRVYWDDI-UHFFFAOYSA-N 1-methyl-2,4-dioxo-1,3-diazinane-5-carboximidamide Chemical compound CN1CC(C(N)=N)C(=O)NC1=O IXPNQXFRVYWDDI-UHFFFAOYSA-N 0.000 claims description 7
- 239000000661 sodium alginate Substances 0.000 claims description 7
- 229940005550 sodium alginate Drugs 0.000 claims description 7
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 6
- 230000001954 sterilising effect Effects 0.000 claims description 6
- 238000003756 stirring Methods 0.000 claims description 6
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims description 5
- 239000004202 carbamide Substances 0.000 claims description 5
- 239000000243 solution Substances 0.000 claims description 5
- 238000004659 sterilization and disinfection Methods 0.000 claims description 4
- 229920001817 Agar Polymers 0.000 claims description 3
- 241000228245 Aspergillus niger Species 0.000 claims description 3
- 240000006439 Aspergillus oryzae Species 0.000 claims description 3
- 235000002247 Aspergillus oryzae Nutrition 0.000 claims description 3
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims description 3
- 244000068988 Glycine max Species 0.000 claims description 3
- 235000010469 Glycine max Nutrition 0.000 claims description 3
- 240000001046 Lactobacillus acidophilus Species 0.000 claims description 3
- 235000013956 Lactobacillus acidophilus Nutrition 0.000 claims description 3
- 240000007594 Oryza sativa Species 0.000 claims description 3
- 235000007164 Oryza sativa Nutrition 0.000 claims description 3
- 239000001888 Peptone Substances 0.000 claims description 3
- 108010080698 Peptones Proteins 0.000 claims description 3
- 241000235342 Saccharomycetes Species 0.000 claims description 3
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 claims description 3
- AYFVYJQAPQTCCC-UHFFFAOYSA-N Threonine Natural products CC(O)C(N)C(O)=O AYFVYJQAPQTCCC-UHFFFAOYSA-N 0.000 claims description 3
- 239000004473 Threonine Substances 0.000 claims description 3
- 240000008042 Zea mays Species 0.000 claims description 3
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 3
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 3
- 239000008272 agar Substances 0.000 claims description 3
- 238000007605 air drying Methods 0.000 claims description 3
- MBLBDJOUHNCFQT-LXGUWJNJSA-N aldehydo-N-acetyl-D-glucosamine Chemical compound CC(=O)N[C@@H](C=O)[C@@H](O)[C@H](O)[C@H](O)CO MBLBDJOUHNCFQT-LXGUWJNJSA-N 0.000 claims description 3
- 235000015278 beef Nutrition 0.000 claims description 3
- 229960003237 betaine Drugs 0.000 claims description 3
- 229940036811 bone meal Drugs 0.000 claims description 3
- 239000002374 bone meal Substances 0.000 claims description 3
- 229940041514 candida albicans extract Drugs 0.000 claims description 3
- KLOIYEQEVSIOOO-UHFFFAOYSA-N carbocromen Chemical compound CC1=C(CCN(CC)CC)C(=O)OC2=CC(OCC(=O)OCC)=CC=C21 KLOIYEQEVSIOOO-UHFFFAOYSA-N 0.000 claims description 3
- 239000003795 chemical substances by application Substances 0.000 claims description 3
- VTNXYFSWTADICO-UHFFFAOYSA-N chromium 2-methylpyridine Chemical compound [Cr].CC1=NC=CC=C1 VTNXYFSWTADICO-UHFFFAOYSA-N 0.000 claims description 3
- 239000002131 composite material Substances 0.000 claims description 3
- 238000001816 cooling Methods 0.000 claims description 3
- 235000005822 corn Nutrition 0.000 claims description 3
- 235000013312 flour Nutrition 0.000 claims description 3
- KWIUHFFTVRNATP-UHFFFAOYSA-N glycine betaine Chemical compound C[N+](C)(C)CC([O-])=O KWIUHFFTVRNATP-UHFFFAOYSA-N 0.000 claims description 3
- 239000002054 inoculum Substances 0.000 claims description 3
- 229940039695 lactobacillus acidophilus Drugs 0.000 claims description 3
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 3
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 3
- 229940099596 manganese sulfate Drugs 0.000 claims description 3
- 239000011702 manganese sulphate Substances 0.000 claims description 3
- 235000007079 manganese sulphate Nutrition 0.000 claims description 3
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 claims description 3
- 239000003595 mist Substances 0.000 claims description 3
- 239000000203 mixture Substances 0.000 claims description 3
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 3
- 235000019796 monopotassium phosphate Nutrition 0.000 claims description 3
- 235000019319 peptone Nutrition 0.000 claims description 3
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 claims description 3
- 239000002504 physiological saline solution Substances 0.000 claims description 3
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 claims description 3
- 229920000053 polysorbate 80 Polymers 0.000 claims description 3
- 239000002994 raw material Substances 0.000 claims description 3
- 235000009566 rice Nutrition 0.000 claims description 3
- 150000003839 salts Chemical class 0.000 claims description 3
- 239000001632 sodium acetate Substances 0.000 claims description 3
- 235000017281 sodium acetate Nutrition 0.000 claims description 3
- 239000007787 solid Substances 0.000 claims description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 3
- 239000003643 water by type Substances 0.000 claims description 3
- 239000012138 yeast extract Substances 0.000 claims description 3
- 239000000843 powder Substances 0.000 claims description 2
- 108090000623 proteins and genes Proteins 0.000 abstract description 10
- 102000004169 proteins and genes Human genes 0.000 abstract description 10
- VYZAMTAEIAYCRO-UHFFFAOYSA-N Chromium Chemical compound [Cr] VYZAMTAEIAYCRO-UHFFFAOYSA-N 0.000 abstract description 4
- 229910052804 chromium Inorganic materials 0.000 abstract description 4
- 239000011651 chromium Substances 0.000 abstract description 4
- 239000000463 material Substances 0.000 abstract description 4
- 238000006243 chemical reaction Methods 0.000 abstract description 3
- 238000010521 absorption reaction Methods 0.000 abstract description 2
- 150000001413 amino acids Chemical class 0.000 abstract description 2
- 230000015572 biosynthetic process Effects 0.000 abstract description 2
- 235000012631 food intake Nutrition 0.000 abstract description 2
- 230000004060 metabolic process Effects 0.000 abstract description 2
- 230000000813 microbial effect Effects 0.000 abstract description 2
- 235000016709 nutrition Nutrition 0.000 abstract description 2
- 230000035764 nutrition Effects 0.000 abstract description 2
- 230000000050 nutritive effect Effects 0.000 abstract description 2
- 108090000765 processed proteins & peptides Proteins 0.000 abstract description 2
- 230000009467 reduction Effects 0.000 abstract description 2
- 241000894007 species Species 0.000 abstract description 2
- 238000003786 synthesis reaction Methods 0.000 abstract description 2
- 230000035899 viability Effects 0.000 abstract description 2
- 241000282898 Sus scrofa Species 0.000 description 19
- 241000282887 Suidae Species 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 235000005911 diet Nutrition 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 235000015241 bacon Nutrition 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000008021 deposition Effects 0.000 description 1
- 230000037213 diet Effects 0.000 description 1
- 230000000378 dietary effect Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 235000012054 meals Nutrition 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 210000003205 muscle Anatomy 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/30—Feeding-stuffs specially adapted for particular animals for swines
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
- A23K10/26—Animal feeding-stuffs from material of animal origin from waste material, e.g. feathers, bones or skin
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/35—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from potatoes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/105—Aliphatic or alicyclic compounds
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/158—Fatty acids; Fats; Products containing oils or fats
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/163—Sugars; Polysaccharides
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/24—Compounds of alkaline earth metals, e.g. magnesium
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/30—Oligoelements
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/113—Acidophilus
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Engineering & Computer Science (AREA)
- Animal Husbandry (AREA)
- Zoology (AREA)
- Food Science & Technology (AREA)
- Biotechnology (AREA)
- Physiology (AREA)
- Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Botany (AREA)
- Mycology (AREA)
- Biomedical Technology (AREA)
- Inorganic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Birds (AREA)
- Sustainable Development (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Fodder In General (AREA)
Abstract
本发明公开了一种提高淮猪瘦肉率的专用饲料制备方法，本发明利用马铃薯渣微生物发酵提取出纯天然营养物质活性肽，具有丰富的营养元素，种类多，并且合理添加限制性氨基酸，能够显著提高饲料转化率，有利于蛋白质的代谢和利用，降低饲料消耗，提高肥育猪住的瘦肉率，赖氨酸能够降低蛋白水平，降低背膘厚度，增加胴体瘦肉率，铬是提高胰岛活性从而刺激组织对葡萄糖吸收的元素，增加铬元素的含量能够改善对葡萄糖的利用，减少蛋白质的降解，从而提高瘦肉率，本发明提供的饲料营养合理，比例适宜，促进猪内蛋白质的合成，促进生长，降低背膘厚度，提高瘦肉率，并且提高猪的抗病力，减少得病率。
Description
技术领域
本发明涉及猪饲料技术领域，尤其涉及一种提高淮猪瘦肉率的专用饲料制备方法。
背景技术
随着人民生活水平的不断提高以及科学技术的发展，人们对饮食结构的合理性更为关注，饮食观念和市场需求的变化，导致对瘦肉型猪需求日益增加，在提高增重速度和饲料转化率上，提高淮猪胴体瘦肉率，改善肉的品质，是当前养猪生产者急需解决的问题。能量水平决定脂肪的沉积，蛋白质水平决定肌肉的增长，生猪在生长发育过程中不同阶段对能量和蛋白质的利用有很大的差别，因此对饲料进行蛋白质和能量水平的调节能够改变生猪的肥瘦程度，并且在一定程度上降低了饲料成本。
发明内容
本发明目的就是为了弥补已有技术的缺陷，提供一种提高淮猪瘦肉率的专用饲料制备方法。
本发明是通过以下技术方案实现的：
一种提高淮猪瘦肉率的专用饲料制备方法，包括以下步骤：
（1）将马铃薯渣在自然条件下风干，粉碎成微粉，然后加入适量的尿素搅拌均匀后用3%的葡萄糖溶液溶解，搅拌均匀后置于90℃的水浴锅中灭菌30min，冷却后备用；
（2）将嗜酸乳杆菌接种于MRS液体培养基在37℃下培养，传代2-3次后，取对数生长期发酵液离心，用等体积生理盐水配置成菌悬液，将酵母菌、黑曲霉和米曲霉活化后取1mL分别接种于50mLPDA培养基内，在28℃下分别培养3天、5天和5天，再分别加入100mL无菌水制得菌悬液备用；
（3）将步骤（2）中制备的菌悬液按照体积比2：2：1：1混合，然后加入到海藻酸钠溶液中，混匀后滴入2%的氯化钙溶液中造粒，固定化1.5-2h后过滤得固定化混合菌制剂；
（4）将步骤（3）得到的固定化复合菌剂按照8%的接种量接种到步骤（1）中马铃薯渣发酵原料中，在28℃下培养72h，发酵结束后在45℃下鼓风干燥粉碎备用；
（5）取米糠40-50份、骨粉8-11份、豆饼20-25份、玉米粉10-15份、干苹果渣6-8份、虾壳粉3-4份、食用盐2-4份、赖氨酸4.8-5.5份、苏氨酸2.2-3.1份、甲基吡啶铬0.002-0.007份、甜菜碱0.4-1份、亚麻酸油1-3份混合搅拌均匀，再加入总重量20-30%步骤（4）制备的产物，混合搅拌即可得到。
一种提高淮猪瘦肉率的专用饲料制备方法，步骤（1）中所述的马铃薯渣与尿素的重量比为19：1。
一种提高淮猪瘦肉率的专用饲料制备方法，步骤（1）中所述的马铃薯渣的质量分数为9%。
一种提高淮猪瘦肉率的专用饲料制备方法，步骤（2）中所述的MRS液体培养基的配方为：蛋白胨10g/L，牛肉膏10g/L，酵母浸粉5g/L，葡萄糖20g/L，乙酸钠2g/L，柠檬酸氢二铵2g/L，磷酸二氢钾2g/L，七水硫酸镁0.58g/L，硫酸锰0.25g/L，吐温801mL，在121℃下高压蒸汽灭菌20min。
一种提高淮猪瘦肉率的专用饲料制备方法，步骤（2）中所述的PDA固体培养基的配方为：马铃薯200g，葡萄糖20g，琼脂20g，自来水1000mL，在121℃下高压蒸汽灭菌20min。
一种提高淮猪瘦肉率的专用饲料制备方法，步骤（3）中所述的海藻酸钠的浓度为2.5wt%，其中菌悬液与海藻酸钠的体积比为1：4。
本发明的优点是：本发明利用马铃薯渣微生物发酵提取出纯天然营养物质活性肽，具有丰富的营养元素，种类多，并且合理添加限制性氨基酸，能够显著提高饲料转化率，有利于蛋白质的代谢和利用，降低饲料消耗，提高肥育猪住的瘦肉率，赖氨酸能够降低蛋白水平，降低背膘厚度，增加胴体瘦肉率，铬是提高胰岛活性从而刺激组织对葡萄糖吸收的元素，增加铬元素的含量能够改善对葡萄糖的利用，减少蛋白质的降解，从而提高瘦肉率，本发明提供的饲料营养合理，比例适宜，促进猪内蛋白质的合成，促进生长，降低背膘厚度，提高瘦肉率，并且提高猪的抗病力，减少得病率。
具体实施方式
一种提高淮猪瘦肉率的专用饲料制备方法，包括以下步骤：
（1）将1Kg马铃薯渣在自然条件下风干，粉碎成微粉，然后加入52.6g尿素搅拌均匀后用3%的葡萄糖溶液溶解，搅拌至马铃薯渣的质量分数为9%时置于90℃的水浴锅中灭菌30min，冷却后备用；
（2）将嗜酸乳杆菌接种于MRS液体培养基在37℃下培养，传代2次后，取对数生长期发酵液离心，用等体积生理盐水配置成菌悬液，将酵母菌、黑曲霉和米曲霉活化后取1mL分别接种于50mLPDA培养基内，在28℃下分别培养3天、5天和5天，再分别加入100mL无菌水制得菌悬液备用；
（3）将步骤（2）中制备的菌悬液按照体积比2：2：1：1混合，然后加入到2.5wt%海藻酸钠溶液中，其中菌悬液与海藻酸钠的体积比为1：4，混匀后滴入2%的氯化钙溶液中造粒，固定化1.5h后过滤得固定化混合菌制剂；
（4）将步骤（3）得到的固定化复合菌剂按照8%的接种量接种到步骤（1）中马铃薯渣发酵原料中，在28℃下培养72h，发酵结束后在45℃下鼓风干燥粉碎备用；
（5）取米糠40份、骨粉8份、豆饼20份、玉米粉10份、干苹果渣6份、虾壳粉3份、食用盐2份、赖氨酸4.8份、苏氨酸2.2份、甲基吡啶铬0.002份、甜菜碱0.4份、亚麻酸油1份混合搅拌均匀得饲料基料，再加入饲料基料质量20%的步骤（4）制备的产物，混合搅拌即可得到。
一种提高淮猪瘦肉率的专用饲料制备方法，步骤（2）中所述的MRS液体培养基的配方为：蛋白胨10g/L，牛肉膏10g/L，酵母浸粉5g/L，葡萄糖20g/L，乙酸钠2g/L，柠檬酸氢二铵2g/L，磷酸二氢钾2g/L，七水硫酸镁0.58g/L，硫酸锰0.25g/L，吐温801mL，在121℃下高压蒸汽灭菌20min。
一种提高淮猪瘦肉率的专用饲料制备方法，步骤（2）中所述的PDA固体培养基的配方为：马铃薯200g，葡萄糖20g，琼脂20g，自来水1000mL，在121℃下高压蒸汽灭菌20min。
Claims (6)
1.一种提高淮猪瘦肉率的专用饲料制备方法，其特征在于，包括以下步骤：
（1）将马铃薯渣在自然条件下风干，粉碎成微粉，然后加入适量的尿素搅拌均匀后用3%的葡萄糖溶液溶解，搅拌均匀后置于90℃的水浴锅中灭菌30min，冷却后备用；
（2）将嗜酸乳杆菌接种于MRS液体培养基在37℃下培养，传代2-3次后，取对数生长期发酵液离心，用等体积生理盐水配置成菌悬液，将酵母菌、黑曲霉和米曲霉活化后取1mL分别接种于50mLPDA培养基内，在28℃下分别培养3天、5天和5天，再分别加入100mL无菌水制得菌悬液备用；
（3）将步骤（2）中制备的菌悬液按照体积比2：2：1：1混合，然后加入到海藻酸钠溶液中，混匀后滴入2%的氯化钙溶液中造粒，固定化1.5-2h后过滤得固定化混合菌制剂；
（4）将步骤（3）得到的固定化复合菌剂按照8%的接种量接种到步骤（1）中马铃薯渣发酵原料中，在28℃下培养72h，发酵结束后在45℃下鼓风干燥粉碎备用；
（5）取米糠40-50份、骨粉8-11份、豆饼20-25份、玉米粉10-15份、干苹果渣6-8份、虾壳粉3-4份、食用盐2-4份、赖氨酸4.8-5.5份、苏氨酸2.2-3.1份、甲基吡啶铬0.002-0.007份、甜菜碱0.4-1份、亚麻酸油1-3份混合搅拌均匀，再加入总重量20-30%步骤（4）制备的产物，混合搅拌即可得到。
2.根据权利要求1所述的一种提高淮猪瘦肉率的专用饲料制备方法，其特征在于，步骤（1）中所述的马铃薯渣与尿素的重量比为19：1。
3.根据权利要求1所述的一种提高淮猪瘦肉率的专用饲料制备方法，其特征在于，步骤（1）中所述的马铃薯渣的质量分数为9%。
4.根据权利要求1所述的一种提高淮猪瘦肉率的专用饲料制备方法，其特征在于，步骤（2）中所述的MRS液体培养基的配方为：蛋白胨10g/L，牛肉膏10g/L，酵母浸粉5g/L，葡萄糖20g/L，乙酸钠2g/L，柠檬酸氢二铵2g/L，磷酸二氢钾2g/L，七水硫酸镁0.58g/L，硫酸锰0.25g/L，吐温801mL，在121℃下高压蒸汽灭菌20min。
5.根据权利要求1所述的一种提高淮猪瘦肉率的专用饲料制备方法，其特征在于，步骤（2）中所述的PDA固体培养基的配方为：马铃薯200g，葡萄糖20g，琼脂20g，自来水1000mL，在121℃下高压蒸汽灭菌20min。
6.根据权利要求1所述的一种提高淮猪瘦肉率的专用饲料制备方法，其特征在于，步骤（3）中所述的海藻酸钠的浓度为2.5wt%，其中菌悬液与海藻酸钠的体积比为1：4。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710742868.2A CN107410737A (zh) | 2017-08-25 | 2017-08-25 | 一种提高淮猪瘦肉率的专用饲料制备方法 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710742868.2A CN107410737A (zh) | 2017-08-25 | 2017-08-25 | 一种提高淮猪瘦肉率的专用饲料制备方法 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107410737A true CN107410737A (zh) | 2017-12-01 |
Family
ID=60433947
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710742868.2A Pending CN107410737A (zh) | 2017-08-25 | 2017-08-25 | 一种提高淮猪瘦肉率的专用饲料制备方法 |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107410737A (zh) |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102499326A (zh) * | 2011-10-31 | 2012-06-20 | 湖南创新生物科技有限公司 | 仔猪后期微生物发酵无抗生素饲料 |
| CN102835551A (zh) * | 2012-08-27 | 2012-12-26 | 广州市番禺区大川饲料有限公司 | 一种高蛋白发酵木薯的制备方法 |
-
2017
- 2017-08-25 CN CN201710742868.2A patent/CN107410737A/zh active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102499326A (zh) * | 2011-10-31 | 2012-06-20 | 湖南创新生物科技有限公司 | 仔猪后期微生物发酵无抗生素饲料 |
| CN102835551A (zh) * | 2012-08-27 | 2012-12-26 | 广州市番禺区大川饲料有限公司 | 一种高蛋白发酵木薯的制备方法 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102669711B (zh) | 一种膳食纤维鹅肉发酵肠 | |
| CN102273706A (zh) | 一种牡蛎蛋白饮料的制备方法 | |
| CN105614828A (zh) | 一种南极磷虾虾酱及其制备方法 | |
| CN101773255A (zh) | 一种发酵生产低值鱼肉香肠的方法 | |
| CN107285929A (zh) | 一种杏鲍菇高效培养基及其制备方法 | |
| CN110521856A (zh) | 一种猪用功能性小肽的制作方法 | |
| CN104824408A (zh) | 一种蛋白桑发酵制备山鸡饲料的方法 | |
| CN104366441B (zh) | 一种牛肉西瓜酱的制备方法 | |
| CN111202231B (zh) | 用于制备酱牛肉的营养骨汤、酱牛肉及其制备方法 | |
| CN101711568A (zh) | 海鲜调味品及其制备方法 | |
| CN105104874A (zh) | 一种生态龟饲料 | |
| CN101720905B (zh) | 一种风味酵母蛋白粉及其制备方法 | |
| CN103907785B (zh) | 一种粥状熟化乳仔猪生物饲料及其制备方法 | |
| CN107410737A (zh) | 一种提高淮猪瘦肉率的专用饲料制备方法 | |
| CN102987330B (zh) | 一种双菌发酵骨泥生产呈味物质的方法 | |
| CN107114273A (zh) | 一种富硒草鱼的养殖方法 | |
| CN105981990A (zh) | 一种清肠排毒棕榈粕鱼饲料及其制备方法 | |
| CN100539871C (zh) | 魔芋精粉方便面 | |
| CN105076816B (zh) | 一种宠物狗零食南瓜条及其制备方法 | |
| KR102645522B1 (ko) | 돈피 콜라겐을 함유하는 반려동물 간식 제조방법 및 이에 의해 제조된 반려동물 간식 | |
| CN108902466A (zh) | 一种水解鱼溶浆及其生产方法与应用 | |
| CN112400760B (zh) | 一种缓解淡水鱼类运输应激的方法 | |
| CN108185350A (zh) | 一种筋韧滑爽的营养面条及其制备方法 | |
| CN106689754A (zh) | 一种海藻粉发酵制备松乳菇发酵棉粕猪中期复合饲料 | |
| CN105533235A (zh) | 一种降低鸡饲养成本的沙棘籽粕饲料及其制备方法 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20171201 |