CN107372370A - Hatching solution for hatching aedes albopictus eggs and hatching method of aedes albopictus eggs - Google Patents
Hatching solution for hatching aedes albopictus eggs and hatching method of aedes albopictus eggs Download PDFInfo
- Publication number
- CN107372370A CN107372370A CN201710803952.0A CN201710803952A CN107372370A CN 107372370 A CN107372370 A CN 107372370A CN 201710803952 A CN201710803952 A CN 201710803952A CN 107372370 A CN107372370 A CN 107372370A
- Authority
- CN
- China
- Prior art keywords
- hatching
- aedes albopictus
- solution
- artemia
- mosquito ovum
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 230000012447 hatching Effects 0.000 title claims abstract description 213
- 241000256173 Aedes albopictus Species 0.000 title claims abstract description 67
- 238000000034 method Methods 0.000 title claims abstract description 35
- 235000013601 eggs Nutrition 0.000 title abstract 10
- 239000000243 solution Substances 0.000 claims abstract description 81
- 239000001888 Peptone Substances 0.000 claims abstract description 42
- 108010080698 Peptones Proteins 0.000 claims abstract description 42
- 235000019319 peptone Nutrition 0.000 claims abstract description 42
- 239000007864 aqueous solution Substances 0.000 claims abstract description 26
- 241000255925 Diptera Species 0.000 claims abstract description 20
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 20
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims abstract description 11
- 241001247197 Cephalocarida Species 0.000 claims description 74
- 102000002322 Egg Proteins Human genes 0.000 claims description 72
- 108010000912 Egg Proteins Proteins 0.000 claims description 72
- 210000004681 ovum Anatomy 0.000 claims description 72
- 108010058643 Fungal Proteins Proteins 0.000 claims description 32
- 238000000855 fermentation Methods 0.000 claims description 32
- 230000004151 fermentation Effects 0.000 claims description 32
- 230000008859 change Effects 0.000 claims description 8
- 241000238582 Artemia Species 0.000 claims description 3
- 238000011534 incubation Methods 0.000 abstract description 19
- 239000007788 liquid Substances 0.000 abstract description 11
- 230000004083 survival effect Effects 0.000 abstract description 11
- 230000008901 benefit Effects 0.000 abstract description 2
- 230000012010 growth Effects 0.000 abstract description 2
- 238000005303 weighing Methods 0.000 abstract description 2
- 239000012530 fluid Substances 0.000 abstract 1
- 238000007789 sealing Methods 0.000 abstract 1
- 230000000052 comparative effect Effects 0.000 description 14
- 230000000384 rearing effect Effects 0.000 description 12
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 description 8
- 230000000694 effects Effects 0.000 description 7
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 6
- 239000001301 oxygen Substances 0.000 description 6
- 229910052760 oxygen Inorganic materials 0.000 description 6
- 241000238631 Hexapoda Species 0.000 description 5
- 230000008569 process Effects 0.000 description 3
- 241000256118 Aedes aegypti Species 0.000 description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- MKYBYDHXWVHEJW-UHFFFAOYSA-N N-[1-oxo-1-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)propan-2-yl]-2-[[3-(trifluoromethoxy)phenyl]methylamino]pyrimidine-5-carboxamide Chemical compound O=C(C(C)NC(=O)C=1C=NC(=NC=1)NCC1=CC(=CC=C1)OC(F)(F)F)N1CC2=C(CC1)NN=N2 MKYBYDHXWVHEJW-UHFFFAOYSA-N 0.000 description 2
- NIPNSKYNPDTRPC-UHFFFAOYSA-N N-[2-oxo-2-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)ethyl]-2-[[3-(trifluoromethoxy)phenyl]methylamino]pyrimidine-5-carboxamide Chemical compound O=C(CNC(=O)C=1C=NC(=NC=1)NCC1=CC(=CC=C1)OC(F)(F)F)N1CC2=C(CC1)NN=N2 NIPNSKYNPDTRPC-UHFFFAOYSA-N 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 238000009825 accumulation Methods 0.000 description 2
- 238000005273 aeration Methods 0.000 description 2
- 238000005265 energy consumption Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 230000009571 larval growth Effects 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 208000001490 Dengue Diseases 0.000 description 1
- 206010012310 Dengue fever Diseases 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 201000006353 Filariasis Diseases 0.000 description 1
- 241000235342 Saccharomycetes Species 0.000 description 1
- 208000003152 Yellow Fever Diseases 0.000 description 1
- 235000021405 artificial diet Nutrition 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 208000025729 dengue disease Diseases 0.000 description 1
- 206010014599 encephalitis Diseases 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 238000011031 large-scale manufacturing process Methods 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 201000004792 malaria Diseases 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000029058 respiratory gaseous exchange Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 230000006641 stabilisation Effects 0.000 description 1
- 238000011105 stabilization Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention relates to an incubation liquid for incubating aedes albopictus eggs and an incubation method of aedes albopictus eggs. The hatching solution is an aqueous solution prepared from yeast peptone and water, and the concentration of the yeast peptone in the aqueous solution is 0.7-1.5 g/L. The hatching method comprises the following steps: fermenting the hatching solution for hatching the aedes albopictus eggs; weighing aedes albopictus eggs, adding the aedes albopictus eggs into an incubation tube, adding the fermented incubation liquid into the incubation tube, sealing a tube cover of the incubation tube, shaking uniformly, standing for incubation, then opening the tube cover of the incubation tube, and transferring the incubated larvae into a feeding tray. The hatching fluid is used for hatching the aedes albopictus eggs under certain conditions, so that the hatching rate of the aedes albopictus eggs can be greatly increased, the hatching rate of active mosquito eggs in 1 hour is high, and the larva growth synchronism is good. The hatching method has the advantages of high larva survival rate, simple operation and low hatching cost.
Description
Technical field
The present invention relates to aedes albopictus artificial diet technique field, is used to hatch aedes albopictus mosquito ovum more particularly to one kind
Artemia hatching solution and aedes albopictus mosquito ovum hatching method.
Background technology
Sterile males release tech is as an emerging technology in control of insect, by cultivating the sterile males of insect simultaneously
Insect breeding place is discharged into, can not be produced offspring with wild female adult post-coitum, so as to reduce or even eliminate the insect.Relative to biography
The Worming strategy of system, the technological accumulation and inheritance is friendly, and other insects and environment are not impacted.Mosquito is as Dengue
The vector of the other pathogens such as heat, stockaded village's card, malaria, yellow fever, filariasis, encephalitis B.In large-scale aedes albopictus
In the works, the hatching of mosquito ovum is one of them important link for male mosquito release, a kind of simple, efficient and steady it is therefore necessary to invent
Fixed mosquito egg hatching method.
Hatching rate of the aedes albopictus mosquito ovum under oxygen-free environment compares aerobic environment much faster, traditional aedes albopictus
The hatching pattern of ovum, it is by thermal de-aeration, heats the water to saturation temperature at ambient pressure, makes solubility drop of the oxygen in water
Low, oxygen escapes from water, forms oxygen-free environment.Then the anaerobic water is transferred quickly in closed container, fills capping, it is cold
But to 25 DEG C~30 DEG C, mosquito ovum needed for weighing, uncap and pour into, shake up, cover again, incubated at a temperature of being placed on 25 DEG C~30 DEG C
Change 1 hour, finally open container, the larva hatched is transferred in rearing tray.The rush that the conventional art is hatched to aedes albopictus
Enter that effect is general, be only capable of within 1 hour hatching less than 80% active mosquito ovum, remaining mosquito ovum is progressively hatched after rearing tray is transferred to
Go out, larval growth poor synchronization.Oxygen content is low in closed container, and the larva first hatched is easily dead.Needed in hatching process
Heating, energy consumption is higher, and cumbersome, and the seal requirement to container is strict.It is unfavorable for the standardized work of large-scale production
And cost control.
The content of the invention
It is white with artemia hatching solution hatching the invention provides a kind of artemia hatching solution for being used to hatch aedes albopictus mosquito ovum based on this
Line yellow-fever mosquito mosquito ovum can significantly accelerate the hatching rate of aedes albopictus mosquito ovum, and incubation rate of the active mosquito ovum in 1 hour is high, makes children
The synchronism of worm growth is good.
Concrete technical scheme is as follows：
A kind of artemia hatching solution for being used to hatch aedes albopictus mosquito ovum, the artemia hatching solution is to prepare to obtain by Yeast protein peptone and water
The aqueous solution, the concentration of Yeast protein peptone is 0.7-1.5g/L in the aqueous solution.
Preferably, the concentration of Yeast protein peptone is 0.8-1.0g/L in the aqueous solution.
Preferably, the concentration of Yeast protein peptone is 0.9g/L in the aqueous solution.
Preferably, the Yeast protein peptone is Angel Yeast peptone.
Present invention also offers a kind of hatching method of aedes albopictus mosquito ovum.This method can significantly accelerate aedes albopictus mosquito
The hatching rate of ovum, incubation rate of the active mosquito ovum in 1 hour is high, makes the synchronism of larval growth good.
Concrete technical scheme is as follows：
A kind of hatching method of aedes albopictus mosquito ovum, comprises the following steps：
It will be fermented for the artemia hatching solution for hatching aedes albopictus mosquito ovum, the artemia hatching solution after being fermented；The artemia hatching solution
It is that the obtained aqueous solution is prepared by Yeast protein peptone and water, the concentration of Yeast protein peptone is 0.7-1.5g/L in the aqueous solution；
Weigh aedes albopictus mosquito ovum to add in hatching pipe, the artemia hatching solution added in the hatching pipe after the fermentation, envelope
The lid of upper hatching pipe, shakes up, and stands hatching, and hatching opens the lid of hatching pipe, the larva hatched is transferred to after terminating
In rearing tray.
Preferably, the artemia hatching solution is to prepare the obtained aqueous solution by Yeast protein peptone and water, yeast in the aqueous solution
The concentration of peptone is 0.8-1.0g/L.
Preferably, the artemia hatching solution is to prepare the obtained aqueous solution by Yeast protein peptone and water, yeast in the aqueous solution
The concentration of peptone is 0.9g/L.
Preferably, the Yeast protein peptone is Angel Yeast peptone.
Preferably, the temperature of the fermentation is 25 DEG C~30 DEG C, and the time of the fermentation is 20-28 hours.
Preferably, the addition of the artemia hatching solution after the fermentation is the 50-80% for hatching pipe volume.
Preferably, the addition of the artemia hatching solution after the fermentation is the 60-70% for hatching pipe volume.
Preferably, the proportioning of the aedes albopictus mosquito ovum and the artemia hatching solution after the fermentation is 1g：150-350mL.
Preferably, the temperature for standing hatching is 25 DEG C~30 DEG C, and the time for standing hatching is small for 0.8-1.2
When.
The hatching method of the artemia hatching solution and aedes albopictus mosquito ovum for hatching aedes albopictus mosquito ovum of the present invention has following
Advantage and beneficial effect：
The present inventor is found surprisingly that using Yeast protein peptone in its long-term experience accumulation and experimentation
The aqueous solution of certain concentration is configured to as the artemia hatching solution of hatching aedes albopictus mosquito ovum, a kind of lineae ablicantes of stability and high efficiency can be obtained
The hatching method of yellow-fever mosquito mosquito ovum, this method can significantly accelerate the hatching rate of aedes albopictus mosquito ovum, and hatching effect is much better than
The hatching method of existing thermal de-aeration.Using the artemia hatching solution of Yeast protein peptone configuration aedes albopictus mosquito ovum, first should during use
Artemia hatching solution is fermented, and the increased saccharomycete bacterium colony of amount reproduction passes through respiration and consumes oxygen in solution, wound during fermentation
Produce a kind of oxygen-free environment that aedes albopictus mosquito ovum can be promoted quickly to hatch.With the artemia hatching solution hatch under certain condition lineae ablicantes she
Mosquito mosquito ovum, it can significantly accelerate the hatching rate of aedes albopictus mosquito ovum, incubation rate of the active mosquito ovum in 1 hour is high, larva
It is good to grow synchronism.
The hatching method of the present invention, the seal of hatching pipe is left without strict demand, and in hatching pipe a certain amount of
Oxygen can be advantageously in will not be because of anoxic and dead in the larva short time that hatching effect, a certain amount of oxygen make to hatch
Die, survival rate of larvae is high, and prerequisite is created for the large-scale standardized raising of further aedes albopictus.
The hatching method of the present invention, the configuration of artemia hatching solution only need Yeast protein peptone and water, and configuration process and hatching
Operating process is all very simple, without energy consumption, greatly reduces hatching cost.
Embodiment
The artemia hatching solution and the aedes albopictus mosquito that are used to hatch aedes albopictus mosquito ovum below in conjunction with specific embodiment to the present invention
The hatching method of ovum is further described in detail.
Used yeast peptone is Angel Yeast peptone FP101 in following examples, and the product is by the height of pure culture
Albumen Saccharomyces cerevisiae, is handled through separation and concentration Yeast protein and compound enzyme effect, a kind of obtained stabilization, safety and nutrition are comprehensive
Peptone.Its content is as follows, total nitrogen：>=10.0%, a- ammonia nitrogen：>=2.5%, moisture：≤ 6.0%, NaCl：≤ 2.0%,
Ash content：≤ 15.0%, pH (2% solution)：5.3-7.2.
Embodiment 1
The present embodiment provides a kind of hatching method of aedes albopictus mosquito ovum, comprises the following steps：
Yeast protein peptone is taken to add water to be configured to the aqueous solution that concentration is 0.9g/L, as artemia hatching solution；
Fermented 24 hours at a temperature of artemia hatching solution is positioned over into 25 DEG C~30 DEG C, the artemia hatching solution after being fermented；
Weigh 0.14g aedes albopictus mosquitos ovum to add in 50mL hatching pipes, incubating after 33.3mL fermentations is added in hatching pipe
Change liquid (artemia hatching solution after fermentation accounts for the 2/3 of hatching pipe volume)；Seal up the lid of hatching pipe, shake up it is rear horizontal positioned, at 25 DEG C
Hatching 1 hour is stood at a temperature of~30 DEG C, the lid of hatching pipe is then opened, the larva hatched is transferred to rearing tray
In.
Embodiment 2
The present embodiment provides a kind of hatching method of aedes albopictus mosquito ovum, comprises the following steps：
Yeast protein peptone is taken to add water to be configured to the aqueous solution that concentration is 0.9g/L, as artemia hatching solution；
Fermented 24 hours at a temperature of artemia hatching solution is positioned over into 25 DEG C~30 DEG C, the artemia hatching solution after being fermented；
Weigh 0.14g aedes albopictus mosquitos ovum to add in 50mL hatching pipes, the hatching added in hatching pipe after 25mL fermentations
Liquid (artemia hatching solution after fermentation accounts for the 50% of hatching pipe volume)；The lid of hatching pipe is sealed up, is shaken up, in 25 DEG C~30 DEG C of temperature
It is lower to stand hatching 1 hour, the lid of hatching pipe is then opened, the larva hatched is transferred in rearing tray.
Embodiment 3
The present embodiment provides a kind of hatching method of aedes albopictus mosquito ovum, comprises the following steps：
Yeast protein peptone is taken to add water to be configured to the aqueous solution that concentration is 0.9g/L, as artemia hatching solution；
Fermented 24 hours at a temperature of artemia hatching solution is positioned over into 25 DEG C~30 DEG C, the artemia hatching solution after being fermented；
Weigh 0.14g aedes albopictus mosquitos ovum to add in 50mL hatching pipes, the hatching added in hatching pipe after 40mL fermentations
Liquid (artemia hatching solution after fermentation accounts for the 80% of hatching pipe volume)；The lid of hatching pipe is sealed up, is shaken up, in 25 DEG C~30 DEG C of temperature
It is lower to stand hatching 1 hour, the lid of hatching pipe is then opened, the larva hatched is transferred in rearing tray.
Embodiment 4
The present embodiment provides a kind of hatching method of aedes albopictus mosquito ovum, comprises the following steps：
Yeast protein peptone is taken to add water to be configured to the aqueous solution that concentration is 0.8g/L, as artemia hatching solution；
Fermented 24 hours at a temperature of artemia hatching solution is positioned over into 25 DEG C~30 DEG C, the artemia hatching solution after being fermented；
Weigh 0.14g aedes albopictus mosquitos ovum to add in 50mL hatching pipes, incubating after 33.3mL fermentations is added in hatching pipe
Change liquid (artemia hatching solution after fermentation accounts for the 2/3 of hatching pipe volume)；The lid of hatching pipe is sealed up, is shaken up, in 25 DEG C~30 DEG C of temperature
Degree is lower to stand hatching 1 hour, then opens the lid of hatching pipe, the larva hatched is transferred in rearing tray.
Embodiment 5
The present embodiment provides a kind of hatching method of aedes albopictus mosquito ovum, comprises the following steps：
Yeast protein peptone is taken to add water to be configured to the aqueous solution that concentration is 1.4g/L, as artemia hatching solution；
Fermented 24 hours at a temperature of artemia hatching solution is positioned over into 25 DEG C~30 DEG C, the artemia hatching solution after being fermented；
Weigh 0.14g aedes albopictus mosquitos ovum to add in 50mL hatching pipes, incubating after 33.3mL fermentations is added in hatching pipe
Change liquid (artemia hatching solution after fermentation accounts for the 2/3 of hatching pipe volume)；The lid of hatching pipe is sealed up, is shaken up, in 25 DEG C~30 DEG C of temperature
Degree is lower to stand hatching 1 hour, then opens the lid of hatching pipe, the larva hatched is transferred in rearing tray.
Comparative example 1
The present embodiment provides a kind of hatching method of aedes albopictus mosquito ovum, comprises the following steps：
Yeast protein peptone is taken to add water to be configured to the aqueous solution that concentration is 2.0g/L, as artemia hatching solution；
Fermented 24 hours at a temperature of artemia hatching solution is positioned over into 25 DEG C~30 DEG C, the artemia hatching solution after being fermented；
Weigh 0.14g aedes albopictus mosquitos ovum to add in 50mL hatching pipes, incubating after 33.3mL fermentations is added in hatching pipe
Change liquid (artemia hatching solution after fermentation accounts for the 2/3 of hatching pipe volume)；The lid of hatching pipe is sealed up, is shaken up, in 25 DEG C~30 DEG C of temperature
Degree is lower to stand hatching 1 hour, then opens the lid of hatching pipe, the larva hatched is transferred in rearing tray.
Comparative example 2
The present embodiment provides a kind of hatching method of aedes albopictus mosquito ovum, comprises the following steps：
Yeast protein peptone is taken to add water to be configured to the aqueous solution that concentration is 0.1g/L, as artemia hatching solution；
Fermented 24 hours at a temperature of artemia hatching solution is positioned over into 25 DEG C~30 DEG C, the artemia hatching solution after being fermented；
Weigh 0.14g aedes albopictus mosquitos ovum to add in 50mL hatching pipes, incubating after 33.3mL fermentations is added in hatching pipe
Change liquid (artemia hatching solution after fermentation accounts for the 2/3 of hatching pipe volume)；The lid of hatching pipe is sealed up, is shaken up, in 25 DEG C~30 DEG C of temperature
Degree is lower to stand hatching 1 hour, then opens the lid of hatching pipe, the larva hatched is transferred in rearing tray.
Comparative example 3
The present embodiment provides a kind of hatching method of aedes albopictus mosquito ovum, comprises the following steps：
Yeast protein peptone is taken to add water to be configured to the aqueous solution that concentration is 0.9g/L, as artemia hatching solution；
Fermented 24 hours at a temperature of artemia hatching solution is positioned over into 25 DEG C~30 DEG C, the artemia hatching solution after being fermented；
Weigh 0.14g aedes albopictus mosquitos ovum to add in 50mL hatching pipes, the hatching added in hatching pipe after 15mL fermentations
Liquid (artemia hatching solution after fermentation accounts for the 30% of hatching pipe volume)；The lid of hatching pipe is sealed up, is shaken up, in 25 DEG C~30 DEG C of temperature
It is lower to stand hatching 1 hour, the lid of hatching pipe is then opened, the larva hatched is transferred in rearing tray.
Comparative example 4
The present embodiment provides a kind of hatching method of aedes albopictus mosquito ovum, comprises the following steps：
Yeast protein peptone is taken to add water to be configured to the aqueous solution that concentration is 0.9g/L, as artemia hatching solution；
Fermented 24 hours at a temperature of artemia hatching solution is positioned over into 25 DEG C~30 DEG C, the artemia hatching solution after being fermented；
Weigh 0.14g aedes albopictus mosquitos ovum to add in 50mL hatching pipes, incubating after 47.5mL fermentations is added in hatching pipe
Change liquid (artemia hatching solution after fermentation accounts for the 95% of hatching pipe volume)；The lid of hatching pipe is sealed up, is shaken up, in 25 DEG C~30 DEG C of temperature
Degree is lower to stand hatching 1 hour, then opens the lid of hatching pipe, the larva hatched is transferred in rearing tray.
The incubation rate of embodiment 1-5 and comparative example 1-4 active mosquito ovum and the statistical result of survival rate of larvae are as follows
Shown in table 1, from result in table：The addition of artemia hatching solution is right in the concentration and hatching pipe of Yeast protein peptone in artemia hatching solution
Incubation rate and survival rate of larvae have conclusive effect.Wherein, embodiment 1-5 effect is much better than comparative example 1-4, and implements
Example 1-3 effect is best, and the incubation rate and survival rate of larvae of active mosquito ovum are very high；Embodiment 4 is due to yeast egg in artemia hatching solution
The concentration of white peptone is relatively low, and the incubation rate of active mosquito ovum is lower than embodiment 1-3, but does not influence its survival rate of larvae；Embodiment 5 by
The concentration of Yeast protein peptone is higher in artemia hatching solution, and the incubation rate of active mosquito ovum is high, but the survival rate of larvae hatched is than implementing
Example 1-3 is low.For comparative example 1 compared to embodiment 1, the concentration of Yeast protein peptone is too high in its artemia hatching solution, causes the larva hatched
Survival rate is extremely low；For comparative example 2 compared to embodiment 1, the concentration of Yeast protein peptone is too low in its artemia hatching solution, causes active mosquito ovum
Incubation rate is extremely low；Comparative example 3 very little, causes the incubation rate of active mosquito ovum low, and incubate compared to embodiment 1, artemia hatching solution addition
The survival rate of larvae dissolved is relatively low；For comparative example 4 compared to embodiment 1, artemia hatching solution addition is too many, causes survival rate of larvae low.
Table 1
| The incubation rate of active mosquito ovum | Survival rate of larvae | |
| Embodiment 1 | 98.34% | 99.32% |
| Embodiment 2 | 95.28% | 99.15% |
| Embodiment 3 | 97.16% | 99.43% |
| Embodiment 4 | 85.67% | 99.28% |
| Embodiment 5 | 98.11% | 74.92% |
| Comparative example 1 | 98.39% | 3.51% |
| Comparative example 2 | 12.53% | 99.19% |
| Comparative example 3 | 35.56% | 83.79% |
| Comparative example 4 | 96.45% | 37.07% |
Embodiment described above only expresses the several embodiments of the present invention, and its description is more specific and detailed, but simultaneously
Can not therefore it be construed as limiting the scope of the patent.It should be pointed out that come for one of ordinary skill in the art
Say, without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to the protection of the present invention
Scope.Therefore, the protection domain of patent of the present invention should be determined by the appended claims.
Claims (10)
1. a kind of be used to hatch the artemia hatching solution of aedes albopictus mosquito ovum, it is characterised in that the artemia hatching solution be by Yeast protein peptone and
Water prepares the obtained aqueous solution, and the concentration of Yeast protein peptone is 0.7-1.5g/L in the aqueous solution.
2. the artemia hatching solution according to claim 1 for being used to hatch aedes albopictus mosquito ovum, it is characterised in that in the aqueous solution
The concentration of Yeast protein peptone is 0.8-1.0g/L.
3. the artemia hatching solution according to claim 2 for being used to hatch aedes albopictus mosquito ovum, it is characterised in that in the aqueous solution
The concentration of Yeast protein peptone is 0.9g/L.
4. the artemia hatching solution for being used to hatch aedes albopictus mosquito ovum according to claim any one of 1-3, it is characterised in that described
Yeast protein peptone is Angel Yeast peptone.
5. a kind of hatching method of aedes albopictus mosquito ovum, it is characterised in that comprise the following steps：
The artemia hatching solution for being used to hatch aedes albopictus mosquito ovum described in claim any one of 1-4 is fermented, after obtaining fermentation
Artemia hatching solution；
Weigh aedes albopictus mosquito ovum to add in hatching pipe, the artemia hatching solution added in the hatching pipe after the fermentation, seal up and incubate
Change the lid of pipe, shake up, stand hatching, hatching opens the lid of hatching pipe, the larva hatched is transferred into raising after terminating
In disk.
6. the hatching method of aedes albopictus mosquito ovum according to claim 5, it is characterised in that the temperature of the fermentation is 25
DEG C~30 DEG C, the time of the fermentation is 20-28 hours.
7. the hatching method of aedes albopictus mosquito ovum according to claim 5, it is characterised in that the artemia hatching solution after the fermentation
Addition for it is described hatching pipe volume 50-80%.
8. the hatching method of aedes albopictus mosquito ovum according to claim 7, it is characterised in that the artemia hatching solution after the fermentation
Addition for it is described hatching pipe volume 60-70%.
9. the hatching method of the aedes albopictus mosquito ovum according to claim any one of 5-8, it is characterised in that the lineae ablicantes she
The proportioning of mosquito mosquito ovum and the artemia hatching solution after the fermentation is 1g：150-350mL.
10. the hatching method of the aedes albopictus mosquito ovum according to claim any one of 5-8, it is characterised in that the standing
The temperature of hatching is 25 DEG C~30 DEG C, and the time for standing hatching is 0.8-1.2 hours.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710803952.0A CN107372370B (en) | 2017-09-08 | 2017-09-08 | Hatching solution for hatching aedes albopictus eggs and hatching method for aedes albopictus eggs |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710803952.0A CN107372370B (en) | 2017-09-08 | 2017-09-08 | Hatching solution for hatching aedes albopictus eggs and hatching method for aedes albopictus eggs |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107372370A true CN107372370A (en) | 2017-11-24 |
| CN107372370B CN107372370B (en) | 2020-08-18 |
Family
ID=60351329
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710803952.0A Active CN107372370B (en) | 2017-09-08 | 2017-09-08 | Hatching solution for hatching aedes albopictus eggs and hatching method for aedes albopictus eggs |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107372370B (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108094768A (en) * | 2017-12-07 | 2018-06-01 | 吉林农业大学 | It cultivates wriggler stalk fermentation matrix and its cultivates wriggler method |
| CN108377976A (en) * | 2018-03-02 | 2018-08-10 | 广州威佰昆生物科技有限公司 | Electric mosquito egg quantifier, mosquito egg quantificational incubator and mosquito egg quantifying method |
| CN108575915A (en) * | 2018-04-18 | 2018-09-28 | 广州威佰昆生物科技有限公司 | Cold storage anesthesia transportation release method for mosquitoes |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101485298A (en) * | 2009-02-06 | 2009-07-22 | 吕军仪 | Cultivation system suitable for animal in cultivation pond and cultivation method thereof |
| US7868222B1 (en) * | 2006-03-10 | 2011-01-11 | University Of Kentucky Research Foundation | Transfected mosquito vectors |
| CN102387702A (en) * | 2009-04-08 | 2012-03-21 | 克里斯蒂安·J·K·霍兰 | Apparatus and method for controlling maturation of aquatically hatched insects |
| CN202310908U (en) * | 2011-11-15 | 2012-07-11 | 中国科学院广州地球化学研究所 | Experimental device for studying chironomid chronic composite toxicity in sediment |
| CN106212855A (en) * | 2016-08-04 | 2016-12-14 | 广州威佰昆生物科技有限公司 | Artificial feed for aedes albopictus larvae and preparation method |
| CN107018956A (en) * | 2017-03-20 | 2017-08-08 | 广州威佰昆生物科技有限公司 | Standardized breeding method for aedes albopictus larvae |
-
2017
- 2017-09-08 CN CN201710803952.0A patent/CN107372370B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7868222B1 (en) * | 2006-03-10 | 2011-01-11 | University Of Kentucky Research Foundation | Transfected mosquito vectors |
| CN101485298A (en) * | 2009-02-06 | 2009-07-22 | 吕军仪 | Cultivation system suitable for animal in cultivation pond and cultivation method thereof |
| CN102387702A (en) * | 2009-04-08 | 2012-03-21 | 克里斯蒂安·J·K·霍兰 | Apparatus and method for controlling maturation of aquatically hatched insects |
| CN202310908U (en) * | 2011-11-15 | 2012-07-11 | 中国科学院广州地球化学研究所 | Experimental device for studying chironomid chronic composite toxicity in sediment |
| CN106212855A (en) * | 2016-08-04 | 2016-12-14 | 广州威佰昆生物科技有限公司 | Artificial feed for aedes albopictus larvae and preparation method |
| CN107018956A (en) * | 2017-03-20 | 2017-08-08 | 广州威佰昆生物科技有限公司 | Standardized breeding method for aedes albopictus larvae |
Non-Patent Citations (1)
| Title |
|---|
| 四川省人民政府地方病防治领导小组办公室等: "《四川省蚊类志》", 30 November 1989 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108094768A (en) * | 2017-12-07 | 2018-06-01 | 吉林农业大学 | It cultivates wriggler stalk fermentation matrix and its cultivates wriggler method |
| CN108094768B (en) * | 2017-12-07 | 2021-05-28 | 吉林农业大学 | Wiggler straw fermentation substrate and wiggler cultivation method thereof |
| CN108377976A (en) * | 2018-03-02 | 2018-08-10 | 广州威佰昆生物科技有限公司 | Electric mosquito egg quantifier, mosquito egg quantificational incubator and mosquito egg quantifying method |
| CN108377976B (en) * | 2018-03-02 | 2024-03-15 | 广州威佰昆生物科技有限公司 | Electric mosquito egg quantitative device, mosquito egg quantitative incubator and mosquito egg quantitative method |
| CN108575915A (en) * | 2018-04-18 | 2018-09-28 | 广州威佰昆生物科技有限公司 | Cold storage anesthesia transportation release method for mosquitoes |
Also Published As
| Publication number | Publication date |
|---|---|
| CN107372370B (en) | 2020-08-18 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN107372370A (en) | Hatching solution for hatching aedes albopictus eggs and hatching method of aedes albopictus eggs | |
| CN106497805B (en) | A kind of cultural method of beauveria bassiana | |
| CN106576895A (en) | Green ecological cultivation method for edible fungi | |
| CN106520584B (en) | Saccharomycete and lactic acid bacteria co-culture with culture medium and preparation method thereof | |
| CN102511452B (en) | Novel method for artificial mass rearing of Amblyseius orientalis | |
| CN102286413B (en) | Preparation method of liquid fermentation medium for bacillus thuringiensis | |
| CN104987156A (en) | Lyophyllum fumosurn culture medium using fermented bran and method for cultivating lyophyllum fumosurn | |
| CN104119126A (en) | Production process for flammulina velutipes medium | |
| CN105948831B (en) | A method of biological and ecological methods to prevent plant disease, pests, and erosion fertilizer is produced using brewex's grains | |
| CN104805136A (en) | Method for producing citric acid by using lignocellulose raw material | |
| CN104322879A (en) | Preparation method of activated ferment straw biscuit feed | |
| CN108076973A (en) | A kind of production method of mushroom concentrated strain | |
| CN107372371A (en) | Breeding method of aedes albopictus oviposition female mosquito | |
| CN110250320A (en) | The preparation method of radix pseudostellariae ferment | |
| CN104046583A (en) | Bacillus thuringiensis liquid culture medium and preparation method thereof | |
| JP2011092041A (en) | Apparatus for continuously culturing and fermenting ethanol-producing microorganism | |
| CN104277978A (en) | Aspergillus niger seed liquid preparation method and citric acid fermentation preparation method | |
| CN102286385A (en) | Method for carrying out solid culturing on beauveria bassiana by stages | |
| CN105176853A (en) | Pichia pastoris for simultaneously producing methanol protein and xylanase and application of pichia pastoris | |
| CN1799363A (en) | Method for preparing bacillus thuringiensis microbiological pesticide by starch waste liquor | |
| CN105062928B (en) | A kind of zymomonas mobilis and its application of resisting high-concentration acetic acid and high concentration furtural | |
| CN104593192B (en) | Process for acid-increasing fermentation of yellow wine | |
| CN111296178A (en) | Method for producing oyster mushrooms by coix seed straws | |
| CN103719633B (en) | A kind of maize straw prepares the method for Yellow meal worm larva feed | |
| CN109548558A (en) | A kind of implantation methods of Pleurotus citrinopileatus |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| CB02 | Change of applicant information | ||
| CB02 | Change of applicant information |
Address after: 510000, Guangzhou City, Guangdong hi tech Industrial Development Zone Science City Kaiyuan Avenue 11, B8 building, fourth floor, Room 401 Applicant after: GUANGZHOU WOLBAKI BIOTECH Co.,Ltd. Address before: 510000, Guangdong province Guangzhou Guangzhou hi tech Industrial Development Zone Science City Kaiyuan Avenue 11, B8 building fourth floor 401 room Applicant before: GUANGZHOU WOLBAKI BIOTECH Co.,Ltd. |
|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |