CN107372233B

CN107372233B - Method for cultivating white cyanea nozakii

Info

Publication number: CN107372233B
Application number: CN201710740830.1A
Authority: CN
Inventors: 董婧; 孙明; 王彬; 李玉龙; 刘修泽
Original assignee: LIAONING OCEAN AND FISHERIES SCIENCE RESEARCH INSTITUTE
Current assignee: LIAONING OCEAN AND FISHERIES SCIENCE RESEARCH INSTITUTE
Priority date: 2017-08-25
Filing date: 2017-08-25
Publication date: 2020-08-21
Anticipated expiration: 2037-08-25
Also published as: CN107372233A

Abstract

The invention belongs to the technical field of artificial breeding of jellyfishes, and particularly relates to a method for cultivating white cyanea nozakii. The method promotes transverse scyphoid generation to generate the disc-shaped bodies by changing culture conditions, solves the technical problem of artificial breeding of the white cyanea nozakii, increases the fragments of the artificially cultured aurea nozakii jellyfish parent as the bait of the white cyanea nozakii from the disc-shaped bodies, breaks through the bait demand bottleneck of the white cyanea nozakii from the disc-shaped body growth to the young jellyfish metamorphosis period, accelerates the breeding speed of the jellyfish, and improves the survival rate of the jellyfish. The invention solves the problem of rapid and low-cost cultivation of the white cyanea nozakii required for appreciation, and fills the blank of the breeding technology for artificially obtaining the jellyfish by the white cyanea nozakii.

Description

Method for cultivating white cyanea nozakii

Technical Field

The invention belongs to the technical field of artificial breeding of jellyfishes, and particularly relates to a method for cultivating white cyanea nozakii.

Background

White Cyanea nozakii kishinouye belongs to the phylum Cnidaria, the class Scyphomedusae (Scyphomedusae), the order Semaeostomeae (Semaeostomeae), the family Cyaneae (Cyaneidae), the genus Cyanea (Cyanea), and is a common warm water jellyfish in offshore China. White cyanea nozakii is also one of main disaster-causing jellyfish species in coastal areas of China, and a large amount of outbreaks occur in sea areas of the Liaodong gulf, the northeast China sea and the south China sea in Bohai sea, so that huge harm is caused to marine ecological environment. Therefore, the conditions of breaking of nets, blocking of meshes, incapability of producing in the traditional fishing ground and the like seriously affect the fishery production in each sea area.

However, as the ornamental jellyfishes become a new favorite in the aquarium market, the white cyanea jellyfish is elegant and extended in water, and the ornamental value is excellent, and the invention of the rapid and low-cost white cyanea cultivating method is urgently needed. The invention patent 'white cyanea scale breeding method' granted publication No. CN 102907360B only discloses a technical scheme for obtaining white cyanea fertilized eggs under the condition of manually controlling illumination intensity, seawater temperature and salinity, and finally obtaining a large amount of scyphistoma after the fertilized eggs are incubated and attached, and does not have related technical content related to the process of regrowth from scyphistoma transverse fission release disc-shaped bodies to young medusa.

Disclosure of Invention

The invention aims to solve the technical problem of providing a quick and efficient white cyanea nozakii cultivation method, which comprises the steps of promoting the white cyanea nozakii scyphistoma to generate cross-cleft reproduction under the manual control condition, releasing the disc-shaped bodies, and using the aurea nozakii disc-shaped bodies as baits to cultivate the white cyanea nozakii disc-shaped bodies to grow to jellyfish adults.

The invention is realized by the following technical scheme: a method for cultivating white cyanea nozakii comprises the steps of culturing white cyanea nozakii scyphistoma, feeding 1 time of newly hatched artemia nauplii every 2 days by using the scyphistoma under the environmental conditions that the water temperature is 6-10 ℃, the salinity is 28-35, the pH value is 7.8-8.3 and the illumination intensity is 400-800 lux, feeding 2-5 artemia nauplii by using each scyphistoma after feeding, and changing water after feeding for 2 hours; slowly raising the water temperature at a speed of 2 ℃ every day, wherein when the water temperature is raised to 18-20 ℃, the scyphistoma is subjected to cross-split reproduction, each scyphistoma is subjected to cross-split reproduction, and a disc-shaped scyphistoma is generated for each time, so that the larger scyphistoma can be continuously subjected to cross-split reproduction for multiple times; the water body culture density of the newly released white cyanea nozakii dish is less than 1/10 ml, 5 artemia nauplii and 5 pieces of aurea nozakii water parent are fed to each ml of water body every day, water is changed after 2 hours of feeding, and the culture conditions of the white cyanea nozakii dish are as follows: the water temperature is 20-25 ℃, the salinity is 25-35, the dish-shaped bodies can grow out to touch the hands after 10 days, and the dish-shaped bodies become young jellyfishes, and then the jellyfishes can be transferred to an jellyfish circulating cylinder for conventional culture.

The white cyanea hybrida scyphistoma is cultured until the diameter of a stem reaches more than 1.5mm before cross-cleft reproduction, and then the temperature is increased to promote the cross-cleft reproduction, so that the released disc-shaped scyphistoma is large in size and high in survival rate.

The artemia nauplii are artificially hatched for 24 hours and have the body length of 200-300 mu m.

The chopped pieces of the aurelia jellyfish are plates of laboratory-cultured aurelia jellyfish or chopped young jellyfish with the volume of about 1-2 mm³The pieces of (4).

The culture water is natural seawater subjected to dark precipitation and sand filtration.

Compared with the prior art, the invention has the beneficial effects that: the invention solves the technical problem of artificial breeding of the white cyanea nozakii, breaks through the bottleneck of bait requirement of the white cyanea nozakii from the growth of a saucer to the metamorphosis stage of the young jellyfish, accelerates the breeding speed of the jellyfish, improves the survival rate of the jellyfish, and solves the requirements of experimental materials and appreciation. Fills the breeding technical blank of artificially obtaining the jellyfish of the white cyanea nozakii.

Detailed Description

The technical solution of the present invention is further described below with reference to examples, but the present invention is not limited to the examples in any way.

Example 1

Culturing white cyanea hybrida scyphistoma in an ecological key laboratory of marine biological resources and ecology in Liaoning province, wherein the culture water temperature is 6-10 ℃, the salinity is 28-35, the pH is 7.8-8.3, and the illumination intensity is 400-800 lux, the culture water is natural seawater subjected to dark precipitation and sand filtration, 1 newly-hatched artemia nauplius is fed for each 2 days, the feeding amount is 5-10 times of the number of the artemia scyphistoma, each fed artemia scyphistoma is guaranteed to eat 2-5 artemia nauplii, and water is changed after feeding for 2 hours.

Culturing white Cyanea cyanea scyphylla scyphistoma according to the culture conditions until the diameter of a handle reaches more than 1.5mm, slowly heating at the speed of 2 ℃ every day until the water temperature is 18-20 ℃, enabling the scyphistoma to generate cross-split reproduction, enabling each scyphistoma to generate a disc-shaped body per time of cross-split reproduction, enabling the large scyphistoma to continuously generate cross-split reproduction for multiple times, and enabling the proportion of the scyphistoma to generate cross-split reproduction to reach more than 90%.

Newly released white cyanea nozakii dish is cultured in the presence of microcirculationIn the container of the circular water flow, the container adopts glass tube micro-aeration to drive water flow micro-circulation, the water body culture density of the newly released white cyanea plate is less than 1/10 ml, 5 artemia nauplii and 5 artemia nauplii with the volume of about 1-2 mm are fed in each ml of water body every day³The pieces of the aurelia aurea water parent body are fed for 2 hours and then water is changed, and the culture conditions of the white cyanea nozakii dish-shaped bodies are as follows: the water temperature is 20-25 ℃, the salinity is 25-35, the dish-shaped bodies can grow out to touch the hands after 10 days, and the dish-shaped bodies become young jellyfishes, and then the jellyfishes can be transferred to an jellyfish circulating cylinder for conventional culture.

The fed artemia nauplii are artificially hatched for 24 hours and have the body length of 200-300 mu m.

The method solves the technical problem of artificial breeding of the white cyanea nozakii by promoting the scyphistoma transverse fission reproduction to generate the dish, breaks through the bottleneck of bait requirement of the white cyanea nozakii from the growth of the dish to the metamorphosis period of the young jellyfish, accelerates the reproduction speed of the jellyfish, and improves the survival rate of the jellyfish.

In this example, a comparison experiment was performed by feeding different baits to the white cyanea dishes, i.e., 30 groups of white cyanea dishes were fed with the artemia nauplii and aurelia pieces of the present invention and the artemia nauplii were fed only according to the prior art by the same feeding method, and the production of the white cyanea dishes was observed, and the obtained experimental data are shown in table 1.

TABLE 1 Effect of different bait species feeding on growth of white cyanea dishes

By adopting the method, the artemia nauplii and the aurelia acuminate fragment baits are fed to the white cyanea acuminate plate, the white cyanea acuminate plate can grow to 5-10 cm in two weeks, the relative growth rate is 13.56%, the white cyanea acuminate plate which is not fed with the aurelia acuminate fragment according to the same feeding method only grows to 3-6 cm in two weeks, and the relative growth rate is only 5.97%.

Claims

1. The method for cultivating the white cyanea nozakii is characterized in that the white cyanea nozakii scyphi is cultured under the environmental conditions that the water temperature is 6-10 ℃, the salinity is 28-35, the pH value is 7.8-8.3 and the illumination intensity is 400-800 lux, 1 newly hatched artemia nauplius is fed to the scyphi every 2 days, 2-5 artemia nauplii are fed to each scyphi after feeding, and water is changed after feeding for 2 hours; slowly raising the water temperature at a speed of 2 ℃ every day, wherein when the water temperature is raised to 18-20 ℃, the scyphistoma is subjected to cross-split reproduction, each scyphistoma is subjected to cross-split reproduction, and a disc-shaped scyphistoma is generated for each time, so that the larger scyphistoma can be continuously subjected to cross-split reproduction for multiple times; the water body culture density of the newly released white cyanea nozakii dish is less than 1/10 ml, 5 artemia nauplii and 5 pieces of aurea nozakii water parent are fed to each ml of water body every day, water is changed after 2 hours of feeding, and the culture conditions of the white cyanea nozakii dish are as follows: the water temperature is 20-25 ℃, the salinity is 25-35, the dish-shaped bodies can grow out to touch hands after 10 days, the dish-shaped bodies become young jellyfishes, and the jellyfishes can be transferred to an jellyfish circulating cylinder for conventional culture;

the chopped pieces of the aurelia jellyfish are laboratory-cultured aurelia jellyfish or chopped young jellyfish with the volume of 1-2 mm³The pieces of (4).

2. The method for cultivating white cyanea nozakii according to claim 1, wherein the white cyanea ophioides is cultured until the diameter of the stem is more than 1.5mm before cross-cleft reproduction, and then the temperature is raised to promote the cross-cleft reproduction.

3. The method for culturing Cyanea leucotricha according to claim 1, wherein the artemia nauplii are artificially hatched for 24 hours and have a body length of 200-300 μm.

4. The method of claim 1, wherein the culture water is natural seawater that has been subjected to dark sedimentation and sand filtration.