CN111771779B - Method for stimulating transverse fissure by inverted jellyfish hydroid - Google Patents
Method for stimulating transverse fissure by inverted jellyfish hydroid Download PDFInfo
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- CN111771779B CN111771779B CN202010734143.0A CN202010734143A CN111771779B CN 111771779 B CN111771779 B CN 111771779B CN 202010734143 A CN202010734143 A CN 202010734143A CN 111771779 B CN111771779 B CN 111771779B
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- 241000242583 Scyphozoa Species 0.000 title claims abstract description 80
- 241000243320 Hydrozoa Species 0.000 title claims abstract description 40
- 238000000034 method Methods 0.000 title claims abstract description 23
- 230000004936 stimulating effect Effects 0.000 title claims abstract description 15
- 239000000758 substrate Substances 0.000 claims abstract description 21
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 21
- 229910001507 metal halide Inorganic materials 0.000 claims abstract description 11
- 150000005309 metal halides Chemical class 0.000 claims abstract description 11
- 238000009395 breeding Methods 0.000 claims abstract description 8
- 230000001488 breeding effect Effects 0.000 claims abstract description 8
- 241000195649 Chlorella <Chlorellales> Species 0.000 claims description 15
- 241001247197 Cephalocarida Species 0.000 claims description 10
- 241000238565 lobster Species 0.000 claims description 10
- 239000011573 trace mineral Substances 0.000 claims description 9
- 239000000203 mixture Substances 0.000 claims description 8
- 238000005286 illumination Methods 0.000 claims description 7
- 241000237852 Mollusca Species 0.000 claims description 6
- 150000001875 compounds Chemical class 0.000 claims description 6
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 claims description 5
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 5
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 claims description 5
- PWHULOQIROXLJO-UHFFFAOYSA-N Manganese Chemical compound [Mn] PWHULOQIROXLJO-UHFFFAOYSA-N 0.000 claims description 5
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 claims description 5
- GVPFVAHMJGGAJG-UHFFFAOYSA-L cobalt dichloride Chemical compound [Cl-].[Cl-].[Co+2] GVPFVAHMJGGAJG-UHFFFAOYSA-L 0.000 claims description 5
- 229910052802 copper Inorganic materials 0.000 claims description 5
- 239000010949 copper Substances 0.000 claims description 5
- JZCCFEFSEZPSOG-UHFFFAOYSA-L copper(II) sulfate pentahydrate Chemical compound O.O.O.O.O.[Cu+2].[O-]S([O-])(=O)=O JZCCFEFSEZPSOG-UHFFFAOYSA-L 0.000 claims description 5
- 239000008103 glucose Substances 0.000 claims description 5
- 229910052749 magnesium Inorganic materials 0.000 claims description 5
- 239000011777 magnesium Substances 0.000 claims description 5
- 229910052748 manganese Inorganic materials 0.000 claims description 5
- 239000011572 manganese Substances 0.000 claims description 5
- CNFDGXZLMLFIJV-UHFFFAOYSA-L manganese(II) chloride tetrahydrate Chemical compound O.O.O.O.[Cl-].[Cl-].[Mn+2] CNFDGXZLMLFIJV-UHFFFAOYSA-L 0.000 claims description 5
- 230000003287 optical effect Effects 0.000 claims description 5
- VWDWKYIASSYTQR-UHFFFAOYSA-N sodium nitrate Inorganic materials [Na+].[O-][N+]([O-])=O VWDWKYIASSYTQR-UHFFFAOYSA-N 0.000 claims description 5
- 238000003756 stirring Methods 0.000 claims description 5
- 239000011701 zinc Substances 0.000 claims description 5
- 229910052725 zinc Inorganic materials 0.000 claims description 5
- RZLVQBNCHSJZPX-UHFFFAOYSA-L zinc sulfate heptahydrate Chemical compound O.O.O.O.O.O.O.[Zn+2].[O-]S([O-])(=O)=O RZLVQBNCHSJZPX-UHFFFAOYSA-L 0.000 claims description 5
- 239000001963 growth medium Substances 0.000 claims description 4
- 241001275783 Mysida Species 0.000 claims 1
- 238000005336 cracking Methods 0.000 abstract description 6
- 230000003203 everyday effect Effects 0.000 abstract description 5
- 241001328003 Mysidacea Species 0.000 description 4
- 239000002131 composite material Substances 0.000 description 4
- 210000000214 mouth Anatomy 0.000 description 4
- 235000013619 trace mineral Nutrition 0.000 description 4
- 230000000694 effects Effects 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 2
- 238000012364 cultivation method Methods 0.000 description 2
- 235000013601 eggs Nutrition 0.000 description 2
- 238000004904 shortening Methods 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000034303 cell budding Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000003086 colorant Substances 0.000 description 1
- 230000004992 fission Effects 0.000 description 1
- ZZUFCTLCJUWOSV-UHFFFAOYSA-N furosemide Chemical compound C1=C(Cl)C(S(=O)(=O)N)=CC(C(O)=O)=C1NCC1=CC=CO1 ZZUFCTLCJUWOSV-UHFFFAOYSA-N 0.000 description 1
- 230000000366 juvenile effect Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 235000012054 meals Nutrition 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 230000036544 posture Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 230000033764 rhythmic process Effects 0.000 description 1
- 210000001563 schizont Anatomy 0.000 description 1
- 230000001568 sexual effect Effects 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 230000009182 swimming Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/20—Culture of aquatic animals of zooplankton, e.g. water fleas or Rotatoria
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The invention discloses a method for stimulating transverse fissure by using an inverted jellyfish hydroid, which comprises the following steps: step one, placing the inverted jellyfish hydroids on an attachment substrate for inverted growth; placing the inverted jellyfish hydroids in a water source, and providing a necessary light source for the breeding of the inverted jellyfish hydroids by using a metal halide lamp; step three, feeding special bait for the hydranth respectively once every day under the light and no light conditions, wherein each time is 2 g/L. The invention can lead the hydranth of the inverted jellyfish to be quickly transversely cracked, shorten the transverse cracking time, improve the quantity and the quality of the generated saucer in the transverse cracking period, reduce the variation rate of the saucer after transverse cracking, obviously improve the yield and the quality of the inverted jellyfish, shorten the culture period of the inverted jellyfish and improve the industrial competitiveness of the inverted jellyfish.
Description
Technical Field
The invention relates to the technical field of cultivation, in particular to a method for stimulating transverse fission by using an inverted jellyfish hydroid.
Background
The inverted jellyfish is also called as a post-immortal inverted jellyfish or a fairy seated inverted jellyfish, belongs to a mollusk door, is coquettish and rich in swimming postures and colorful gorgeous colors, symbolizes elegant life and noble quality, and is the life rich in flexibility and noble quality in the sea, so that the inverted jellyfish becomes fashionable pets of various families in recent years, and the ornamental jellyfish, particularly the inverted jellyfish, is a relatively mature family aquarium variety in Japan, Singapore and other countries. In developed countries such as europe and the united states, the inverted jellyfishes are also commonly used for arrangement in venues of high-grade business entertainment places due to the expensive price, the gorgeous color and the elegant rhythm under the illumination of light, and are a symbol of wealth and grade. The user can see the figure of the user in some high-grade restaurant bars, and the user can enjoy the inverted jellyfish and have a meal with friends, so that the effect is excellent.
The inverted jellyfish comprises two life generations with different forms, namely an asexual hydrozoan generation for camping and fixing life and a sexual inverted jellyfish generation for camping and floating life, wherein the inverted jellyfish is a female and male variant, sperms are discharged from the mouth of a male individual and enter the body through the oral cavity of a female individual to be combined with eggs, fertilized eggs flow to the edge of the mouth and develop into billow larvae, the billow larvae leave the female inverted jellyfish, foreign matters are attached to the front end of the female inverted jellyfish to develop into early stage scyphistozoan, the scyphistozoan continuously generates new scyphistozoan by budding, and after a long period of nutrition, the pot mouth juvenile develops into continuous transverse division to form a plurality of stacked discs which become transverse fissures, the transverse fissured discs fall off to form discs which further develop into the young inverted jellyfish.
With the maturity of inverted jellyfish breeding technology and inverted jellyfish breeding equipment, inverted jellyfishes have entered the domestic thousands of households as fashionable new pets, and the demand of the aquatic animal market for inverted jellyfishes is increasing day by day. In addition, because of the special symbiotic relationship between the inverted jellyfishes and the zooxanthella, the inverted jellyfishes are used as model organisms for researching the action mechanism of 'plant-photosynthesis-animal', are widely applied to the field of scientific research and have extremely high market demands. However, the process of the inverted jellyfish from early hydranth to transverse schizont is long, so that the yield of the inverted jellyfish is lost seasonally, and the continuous supply of the inverted jellyfish market is seriously influenced.
In the conventional solutions, the variation time is shortened by increasing the temperature based on the living environment of the inverted jellyfish, but the conventional solutions are often poor in effect, difficult to stably stimulate the formation of the transverse fissures of the inverted jellyfish, and incapable of stably shortening the time period from the hydroid to the transverse fissures. The continuous supply of the inverted jellyfishes all the year round can be ensured only by stably shortening the time period from the inverted jellyfishes to the transverse cleft bodies, and the market demand of the inverted jellyfishes is improved.
Disclosure of Invention
The invention aims to provide a method for stimulating transverse fissure by inverting a jellyfish hydroid, so as to solve the problems in the background technology.
In order to achieve the purpose, the invention provides the following technical scheme:
a method for stimulating transverse fissure by inverting a jellyfish hydroid is characterized by comprising the following steps:
step one, placing the inverted jellyfish hydroids on an attachment substrate for inverted growth;
placing the inverted jellyfish hydroids in a water source, and providing a necessary light source for the breeding of the inverted jellyfish hydroids by using a metal halide lamp;
step three, feeding special bait for the hydranth respectively once every day under the light and no light conditions, wherein each time is 2 g/L.
Preferably, the attachment substrate is a mollusc shell.
Preferably, the method for inverted growth of the inverted jellyfish hydroids comprises the following steps: the adhering substrate is obliquely arranged, and the hydranth is adhered to the ground of the adhering substrate and grows in an inverted state.
Preferably, the water quality condition of the water source is pH7.8-8.8, the transparency is 50-80cm, and the water flow is 20L/h.
Preferably, the illumination intensity of the metal halide lamp is 400lx-800lx, and the illumination time is 12 h/day.
Preferably, the specialty bait is a composite of chlorella, mysidacea, artemia larvae and lobster.
Preferably, the chlorella is cultured in the form of a culture solution, wherein the culture solution is Na with the mass fraction of 5mg/L2SO33g/L glucose, 75mg/L NaNO3Trace elements, 3g/L zinc sulfate heptahydrate (ZnSO)4·7H2O), 1g/L of cobalt chloride (CoCl)2) 18g/L of manganese chloride tetrahydrate (MnCl)2·4H2O), 1g/L copper sulfate pentahydrate (CuSO)4·5H2O), and the optical density value (OD680) of the culture solution was 7.0.
Preferably, the trace elements are zinc, magnesium, manganese and copper according to the proportion of 3:1:18: 1.
Preferably, the mysid, the artemia larvae and the lobster are mixed according to the weight ratio of 1: 2: 1, mixing, and then feeding into a stirring pulverizer to pulverize to form the minced fillet compound.
Preferably, the feeding bait dosage of the special bait is as follows: 5ml/L of chlorella culture solution and 20g/L of minced fillet mixture.
Compared with the prior art, the invention has the following beneficial effects:
the invention can lead the hydranth of the inverted jellyfish to be quickly transversely cracked, shorten the transverse cracking time, improve the quantity and the quality of the generated saucer in the transverse cracking period, reduce the variation rate of the saucer after transverse cracking, obviously improve the yield and the quality of the inverted jellyfish, shorten the culture period of the inverted jellyfish and improve the industrial competitiveness of the inverted jellyfish.
Detailed Description
The technical solutions in the embodiments of the present invention are clearly and completely described below with reference to specific embodiments, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1:
the method for stimulating transverse fissure by inverting the jellyfish hydroid is characterized by comprising the following steps of:
step one, placing the inverted jellyfish hydroids on an attachment substrate for inverted growth;
placing the inverted jellyfish hydroids in a water source, and providing a necessary light source for the breeding of the inverted jellyfish hydroids by using a metal halide lamp;
step three, feeding special bait for the hydranth respectively once every day under the light and no light conditions, wherein each time is 2 g/L.
The attachment substrate of this example is a mollusk shell.
The method for inverted growth of the hydranth of the inverted jellyfish in the embodiment comprises the following steps: the adhering substrate is obliquely arranged, and the hydranth is adhered to the ground of the adhering substrate and grows in an inverted state.
The water quality of the water source in this example was pH7.8, the transparency was 50cm, and the water flow rate was 20L/h.
The light intensity of the metal halide lamp of the embodiment is 400lx, and the light time is 12 h/day.
The specialty bait of this example was a composite of chlorella, mysidacea, artemia larvae and lobster.
The chlorella of this example was cultured in the form of a culture medium containing Na in an amount of 5mg/L by mass2SO33g/L glucose, 75mg/L NaNO3Trace elements, 3g/L zinc sulfate heptahydrate (ZnSO)4·7H2O), 1g/L of cobalt chloride (CoCl)2) 18g/L of manganese chloride tetrahydrate (MnCl)2·4H2O), 1g/L copper sulfate pentahydrate (CuSO)4·5H2O), and the optical density value (OD680) of the culture solution was 7.0.
The trace elements of the embodiment are zinc, magnesium, manganese and copper according to the mixture ratio of 3:1:18: 1.
The mysid, the artemia larvae and the lobster in the embodiment are mixed according to the weight ratio of 1: 2: 1, mixing, and then feeding into a stirring pulverizer to pulverize to form the minced fillet compound.
The feeding bait dosage of the special bait in the embodiment is as follows: 5ml/L of chlorella culture solution and 20g/L of minced fillet mixture.
Example 2:
the method for stimulating transverse fissure by inverting the jellyfish hydroid is characterized by comprising the following steps of:
step one, placing the inverted jellyfish hydroids on an attachment substrate for inverted growth;
placing the inverted jellyfish hydroids in a water source, and providing a necessary light source for the breeding of the inverted jellyfish hydroids by using a metal halide lamp;
step three, feeding special bait for the hydranth respectively once every day under the light and no light conditions, wherein each time is 2 g/L.
The attachment substrate of this example is a mollusk shell.
The method for inverted growth of the hydranth of the inverted jellyfish in the embodiment comprises the following steps: the adhering substrate is obliquely arranged, and the hydranth is adhered to the ground of the adhering substrate and grows in an inverted state.
The water quality of the water source in this example was pH8.8, the transparency was 80cm, and the water flow rate was 20L/h.
The illumination intensity of the metal halide lamp in the embodiment is 400lx-800lx, and the illumination time is 12 h/day.
The specialty bait of this example was a composite of chlorella, mysidacea, artemia larvae and lobster.
The chlorella of this example was cultured in the form of a culture medium containing Na in an amount of 5mg/L by mass2SO33g/L glucose, 75mg/L NaNO3Trace elements, 3g/L zinc sulfate heptahydrate (ZnSO)4·7H2O), 1g/L of cobalt chloride (CoCl)2) 18g/L of manganese chloride tetrahydrate (MnCl)2·4H2O), 1g/L copper sulfate pentahydrate (CuSO)4·5H2O), and the optical density value (OD680) of the culture solution was 7.0.
The trace elements of the embodiment are zinc, magnesium, manganese and copper according to the mixture ratio of 3:1:18: 1.
The mysid, the artemia larvae and the lobster in the embodiment are mixed according to the weight ratio of 1: 2: 1, mixing, and then feeding into a stirring pulverizer to pulverize to form the minced fillet compound.
The feeding bait dosage of the special bait in the embodiment is as follows: 5ml/L of chlorella culture solution and 20g/L of minced fillet mixture.
Example 3:
the method for stimulating transverse fissure by inverting the jellyfish hydroid is characterized by comprising the following steps of:
step one, placing the inverted jellyfish hydroids on an attachment substrate for inverted growth;
placing the inverted jellyfish hydroids in a water source, and providing a necessary light source for the breeding of the inverted jellyfish hydroids by using a metal halide lamp;
step three, feeding special bait for the hydranth respectively once every day under the light and no light conditions, wherein each time is 2 g/L.
The attachment substrate of this example is a mollusk shell.
The method for inverted growth of the hydranth of the inverted jellyfish in the embodiment comprises the following steps: the adhering substrate is obliquely arranged, and the hydranth is adhered to the ground of the adhering substrate and grows in an inverted state.
The water quality of the water source of this example was pH7.8-8.8, transparency 65cm, and water flow rate 20L/h.
The light intensity of the metal halide lamp in this example was 600lx, and the light time was 12 h/day.
The specialty bait of this example was a composite of chlorella, mysidacea, artemia larvae and lobster.
The chlorella of this example was cultured in the form of a culture medium containing Na in an amount of 5mg/L by mass2SO33g/L glucose, 75mg/L NaNO3Trace elements, 3g/L zinc sulfate heptahydrate (ZnSO)4·7H2O), 1g/L of cobalt chloride (CoCl)2) 18g/L of manganese chloride tetrahydrate (MnCl)2·4H2O), 1g/L copper sulfate pentahydrate (CuSO)4·5H2O), and the optical density value (OD680) of the culture solution was 7.0.
The trace elements of the embodiment are zinc, magnesium, manganese and copper according to the mixture ratio of 3:1:18: 1.
The mysid, the artemia larvae and the lobster in the embodiment are mixed according to the weight ratio of 1: 2: 1, mixing, and then feeding into a stirring pulverizer to pulverize to form the minced fillet compound.
The feeding bait dosage of the special bait in the embodiment is as follows: 5ml/L of chlorella culture solution and 20g/L of minced fillet mixture.
Experimental example 1:
the experimental example comprises an experimental group and a control group, wherein 50 inverted jellyfish hydroids with similar sizes and shapes are selected in the two groups respectively, the experiment of the jellyfish hydroids-transverse cleft is carried out, and the experimental group is repeated for three times: the method of embodiment 3 of the invention; the control group was: conventional market technologies exist.
The time from 50 standing upside down the jellyfish hydroids to the transverse fissure stage, and the number of discs produced in 5 days:
| experimental group | Control group | |
| Time to release the disc | 20±3day | 53±2day |
| Number of plates produced in 5 days | 315±33day | 435±27day |
| Rate of change of discoid body | 2.3±0.237％ | 0.9±0.219％ |
| Average diameter of dish | 1.7±0.07mm | 2.8±0.11mm |
Note: each experiment was repeated 3 times and all data were examined for normality.
The cultivation method for the reversed jellyfish hydroid body to stimulate the transverse rupture can obviously shorten the time for generating the reversed jellyfish transverse rupture body, improve the generation quantity of the reversed jellyfish dishes, increase the average diameter of the reversed jellyfish dishes, reduce the variation of the reversed jellyfish dishes, greatly reduce the loss of manpower and material resources and improve the survival rate of the reversed jellyfish. The cultivation method has the advantages of reducing cultivation cost and cultivation period of the inverted jellyfishes, being low in cost, good in universality, remarkable in effect, suitable for large-scale popularization and application, and beneficial to promoting low-cost, efficient, continuous and stable cultivation of the inverted jellyfishes.
It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein.
Furthermore, it should be understood that although the present description refers to embodiments, not every embodiment may contain only a single embodiment, and such description is for clarity only, and those skilled in the art should integrate the description, and the embodiments may be combined as appropriate to form other embodiments understood by those skilled in the art.
Claims (7)
1. A method for stimulating transverse fissure by inverting a jellyfish hydroid is characterized by comprising the following steps:
step one, placing the inverted jellyfish hydroids on an attachment substrate for inverted growth;
placing the inverted jellyfish hydroids in a water source, and providing a necessary light source for the breeding of the inverted jellyfish hydroids by using a metal halide lamp;
step three, feeding special bait for the hydranth respectively once a day under the light and no light conditions, wherein each time is 2 g/L;
the special bait is a compound of chlorella, mysidacea, artemia larvae and lobster;
the mysid, the artemia larvae and the lobster are mixed according to the weight ratio of 1: 2: 1, mixing, and then feeding into a stirring and crushing machine to be crushed to form a minced fillet compound;
the feeding bait dosage of the special bait is as follows: 5ml/L of chlorella culture solution and 20g/L of minced fillet mixture.
2. The method of claim 1, wherein said attachment substrate is a mollusk shell.
3. The method for stimulating the transverse fissure of the inverted hydrozoan of claim 1, wherein the method for the inverted growth of the inverted hydrozoan comprises the following steps: the adhering substrate is obliquely arranged, and the hydranth is adhered to the ground of the adhering substrate and grows in an inverted state.
4. The method for stimulating the transverse rupture of the hydroid of inverted medusa as claimed in claim 1, wherein the water source has a water quality condition of pH7.8-8.8, a transparency of 50-80cm and a water flow size of 20L/h.
5. The method for stimulating transverse rupture of an inverted jellyfish hydroid as claimed in claim 1, wherein said metal halide lamp is used for an illumination time of 12 h/day and an illumination intensity of 400lx-800 lx.
6. The method for stimulating transverse rupture of the hydroid of Megasphaea according to claim 1, wherein said Chlorella is cultured in the form of a culture medium containing Na with a mass fraction of 5mg/L2SO33g/L glucose, 75mg/L NaNO3Trace elements, 3g/L zinc sulfate heptahydrate (ZnSO)4·7H2O), 1g/L of cobalt chloride (CoCl)2) 18g/L of manganese chloride tetrahydrate (MnCl)2·4H2O), 1g/L copper sulfate pentahydrate (CuSO)4·5H2O), and the optical density value (OD680) of the culture solution was 7.0.
7. The method for stimulating transverse rupture of an inverted hydrozoan of claim 6, wherein the microelements comprise zinc, magnesium, manganese and copper at a ratio of 3:1:18: 1.
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