CN107353048A - A kind of straw decomposing inoculant and preparation method thereof and the method by the decomposed stalk batch making solid-state biological organic fertilizer of this decomposing agent - Google Patents
A kind of straw decomposing inoculant and preparation method thereof and the method by the decomposed stalk batch making solid-state biological organic fertilizer of this decomposing agent Download PDFInfo
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- CN107353048A CN107353048A CN201710545600.XA CN201710545600A CN107353048A CN 107353048 A CN107353048 A CN 107353048A CN 201710545600 A CN201710545600 A CN 201710545600A CN 107353048 A CN107353048 A CN 107353048A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
- C05F11/08—Organic fertilisers containing added bacterial cultures, mycelia or the like
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D1/00—Fertilisers containing potassium
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/10—Solid or semi-solid fertilisers, e.g. powders
- C05G5/12—Granules or flakes
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- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Pest Control & Pesticides (AREA)
- Soil Sciences (AREA)
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Abstract
Method the invention discloses a kind of straw decomposing inoculant and preparation method thereof and by the decomposed stalk batch making solid-state biological organic fertilizer of this decomposing agent, the straw decomposing inoculant include the raw material of following percentage by weight：Bacillus subtilis 10~15%, bacillus megaterium 15~20%, trichoderma harzianum 20~25%, trichoderma viride 20~25%, bacillus thuringiensis 15~25%.Straw decomposing inoculant is mixed with the stalk after crushing, heap fermentation is built and the solid-state biological organic fertilizer is can obtain after cold extrusion is granulated, dried.Its particle is uniform, uniform specification, the even action with arable land, can be mass and sells, be applied to after arable land, has suppression disease, growth promoting, makes fertilizer, removing toxic substances and other effects；And organic nutriment in a fertilizer that organic material decomposition is plant available can be provided precious Organic nutrient for crop growth, strengthen fertilizer efficiency, promote crop to mushroom out.
Description
Technical field
Consolidate the present invention relates to a kind of straw decomposing inoculant and preparation method thereof and by the decomposed stalk batch making of this decomposing agent
The method of state biological organic fertilizer.
Background technology
Current agriculture vegetables greenhouse cultivation long-term use agricultural chemicals and largely use chemical fertilizer, cause soil organic matter content by
Year reduce, cause soil it is hardened, acidifying, eelworm harm it is serious, the plantation of single variety causes continuous cropping obstacle, Farming again
Substantial amounts of stalk is produced due to not effective processing mode, generally abandons or burns and cause huge environmental pollution.
Modern high-new biotechnology is complied with the nature rule, and traditional production technology is coordinated using advanced scientific basic, oneself
The processing and utilization of stalk can be stably grasped, succeeds in developing the production technology of a new generation.
Using biotechnology in planting industry, quality and economic benefit can be lifted and improve environment and soil, in plantation
It can effectively reach disease-resistant, pest-resistant and solve crops continuous cropping obstacle.Stalk caused by grain class crop can be made organic fertilizer, answer
The products such as Hefei material, not only create second of income, but also have reached the target of resource reclaim recycling, and overall efficiency shows
Write.Organic fertilizer collocation microorganism science and technology, in agricultural crops production work, it can reach high-quality, high yield, free of contamination green
Color agricultural achievements.
Biotechnology can make agricultural wastes, planting industry, environmental protection industry all linked with one another, exhaustive, and exactly ecological industry is sent out
The successful key of exhibition.
The content of the invention
It is the invention discloses a kind of straw decomposing inoculant and preparation method thereof and decomposed by this decomposing agent based on background above
The method of stalk batch making solid-state biological organic fertilizer.With high temperature caused by the flora advantage in straw decomposing agent and Rapid Fermentation
To eliminate the pest and disease damage in stalk, while the Organic nutrient of stalk is decomposed into organic fertilizer, while bred a large amount of beneficial
Microorganism and the ventilative and fertilizer conservation effect for increasing soil using the stalk after fermentation as carrier, to recover soil ecology balance, suppress
Pest and disease damage, promote plant growth, such a method can largely recycle stalk, can increase the yield and ring of crops
Protect.
The technical scheme that the present invention takes is：
A kind of straw decomposing agent, include the raw material of following percentage by weight：Bacillus subtilis 10~15%, huge gemma
Bacillus 15~20%, trichoderma harzianum 20~25%, trichoderma viride 20~25%, bacillus thuringiensis 15~25%.
Present invention also offers the preparation method of the straw decomposing inoculant, comprise the following steps：
(1) by bacillus subtilis, bacillus megaterium, trichoderma harzianum, trichoderma viride, bacillus thuringiensis point
It is not seeded on solid agar medium, cultivates；
(2) strain after step (1) is cultivated, is inoculated in fluid nutrient medium respectively, cultivates；
(3) strain after step (2) is cultivated is respectively placed in fermentation tank, and volume production culture bacterium number into zymotic fluid reaches 10
~15 × 1010Individual/ml；
(4) strain after step (3) volume production culture is mixed by formula ratio, intersects antagonism culture, straw decomposing inoculant is made.
Condition of culture in the step (1) is：25~30 DEG C of 36~48h of culture of temperature.
In the step (2), Liquid Culture based component is：Sucrose 10g, glucose 10g, K2HPO40.2g, CaCO32g,
NaCl 0.4g, distilled water are settled to 1L, and the Liquid Culture based component used in five kinds of strains is identical；Condition of culture is 28~30 DEG C
Under, shaking flask speed 60r/min, cultivate 36~48h.
In the step (3), fermentation medium components are：30~60g/L of glucose, 50~100g/L of molasses, urea 1~
10g/L, KH2PO45~10g/L, remaining is distilled water；PH is 6.8~7.2.The inoculum concentration of strain is culture medium weight in fermentation tank
The 3~10% of amount.Each composition act as in culture medium：Glucose provides the nutrient that strain spreads cultivation volume production initial stage；Molasses provide
Strain spreads cultivation the nutrient of volume production middle and later periods；Urea provides the nitrogen that strain spreads cultivation needed for volume production；Potassium dihydrogen phosphate provides strain
Phosphorus, the potassium element to spread cultivation needed for volume production.
In the step (4), intersect antagonism culture, used medium composition is：20~40g/L of glucose, molasses 30~
50g/L, urea 5~12g/L, P2O55~10g/L, KH2PO42~10g/L, remaining is distilled water, and pH is 6.8~7.2.Training
Each composition supported in base is act as：Glucose：The nutrient that strain spreads cultivation volume production initial stage is provided；Molasses：Strain is provided to spread cultivation volume production
The nutrient of middle and later periods；Urea：The nitrogen that strain spreads cultivation needed for volume production is provided；Potassium dihydrogen phosphate and phosphorus pentoxide：Bacterium is provided
Phosphorus, the potassium element that kind spreads cultivation needed for volume production.
Condition of culture in the step (3) and (4) is：120h is cultivated at 30 DEG C, is stirred in incubation per 12h
5min, air 10min is filled per 2h, and air mass flow is liquid per ton 2 cubic metres of inflation per minute.
After intersecting antagonism culture, each mutual antagonism competitive growth of strain, beneficial microbe colony is formed, in this flora
In, bacillus subtilis and bacillus megaterium can suppress and kill harmful bacteria in soil, pathogen；Trichoderma harzianum and
Trichoderma viride can decomposing straw fiber, quick composting stalk；Bacillus thuringiensis suppresses and killed the insect in soil
And worm's ovum, each strain of the above is intended to be limited to specify within the scope of, not so easily because of its flora imbalance, obtained straw decomposing
The effect of agent, can substantially reduce.
Present invention also offers a kind of preparation method of solid-state biological organic fertilizer, comprise the following steps：
A. the straw decomposing inoculant described in claim 1 is mixed with the stalk after crushing, builds heap and ferment three days；
B. N, P, K fertilizer are added, continues to ferment, obtains the good stalk base-material of fermentation maturity；
C. the good stalk base-material of fermentation maturity is screened through screen cloth, after incorporation cornstarch, N fertilizer, P fertilizer and K fertilizer, adjusting body
It is that water content is 25%~35%, the solid-state biological organic fertilizer is can obtain after cold extrusion is granulated, dried.
In the step a, the addition of straw decomposing inoculant is the 0.3~0.5% of stalk weight, builds the condition of heap fermentation
For：After 48 hrs, after reaching 60~65 DEG C, once, water content is maintained at 50%~70% to fermentation temperature, pH value for daily turning
For 6.5~7.5, ferment three days；Pathogen, harmful bacteria, worm's ovum etc. are killed with high temperature caused by fermentation.
Further, the length of the stalk after the crushing is 3~6cm, and the harvesting of crops at present is using joint mostly
Harvester, the setting of the straw harvesting length of the united reaper of most manufacturer production is also within the scope of this, therefore entering
When row stalk builds heap fermentation, the agricultural crop straw after united reaper harvesting is without further pulverization process, after recovery
It can be used, eliminate the duplication of labour.
In the step b, the addition of N, P, K fertilizer is in stalk per ton：N fertilizer 2~5kg, P fertilizer 0.5~1.5kg, K fertilizer
0.2~0.5kg；Fermentation condition is：After tenth day, temperature is 30~40 DEG C, and water content is 30%~50%, every turning one in two days
Secondary, after fermenting the 15th day, temperature is identical with room temperature, water content 15%.What N, P, K were fertile is incorporated as mistake of the microorganism in fermentation
Nitrogen, phosphorus, potassium required for being supplemented in journey.
In the step c, uniformly incorporation corn fine powder 40kg, N fertilizer 3kg, P fertilizer 1.5kg, K is fertile in stalk base-material per ton
0.5kg.The addition of corn fine powder adds the viscosity of stalk base-material, is advantageous to the mistake that solid-state biological organic fertilizer is granulated in cold extrusion
More preferable granulating and forming in journey；N, the addition of P, K fertilizer ensure that the nutrient supply of the microorganism in solid-state biological organic fertilizer particle,
It is set to keep lasting activity and vitality.
In the step c, it is pair roller type cold extrusion organic granular fertilizer forming machine that cold extrusion, which is granulated equipment used, is had：
Two bearings；
Two rollers, the roller both ends are installed in rotation on two bearings respectively, and two roller outer peripheral faces connect
Touch；
Roller surfaces are provided with a series of dome-type grooves, and in two roller relative rotational motions, two rollers are in contact
Outer peripheral face on dome-type groove it is corresponding formed a ball-type groove；
Described two rollers respectively have the roll shaft of one end to stretch out the bearing, and a gear is respectively fixed with the roll shaft,
Two gears of two rollers are meshed；
One roller is connected with power set；
Hopper, it is arranged on above the roller, between two rollers of discharging opening face of the hopper.
Also there is material-falling hopper, the material-falling hopper is located at below the roller, and the length of the material-falling hopper is more than the roller
Length.
The length of the hopper is less than the length of the roller.
The power set are motor, and the rotating shaft of the motor and the roll shaft of roller connect.
Rolled by the way that two rollers are relative, the dome-type groove on two rollers forms a complete ball-type groove, has
Machine fertilizer is molded in ball-type groove, and shaping efficiency is high, the organic granular fertilizer regular shape of formation, uniform specification.
Present invention also offers a kind of solid-state biological organic fertilizer being prepared according to above-mentioned preparation method.This solid-state biology
In organic fertilizer, agricultural crop straw is mutually cooperateed with and played a role with straw decomposing inoculant and N, P, K fertilizer, overcome soil-borne pathogen to accumulate,
The soil organism is supplemented, optimizes the crumb structure of soil, enables water conservation, draining, breathe freely, make crop root system flourishing, plant
Strain is healthy and strong, not only environmental protection but also volume increase.
Compared with prior art, the preparation method of solid-state biological organic fertilizer disclosed by the invention, heap fermentation is built in stalk
During, it is no worm, odorless, nontoxic, hot fermentation heat is produced during fermentation, beneficial to eliminating pathogen remaining in stalk and worm's ovum,
The fermentation curing time is shorter, greatlys save artificial, factory building, water power and management cost.
Solid-state biological organic fertilizer disclosed by the invention is made using pair roller type cold extrusion organic granular fertilizer forming machine through cold extrusion
Particle shape granulates product, its particle uniformly, uniform specification, the even action with arable land, can be mass and sell, solid-state biology
The use of organic fertilizer can effectively be alleviated because of the soil hardening caused by a large amount of uses of chemical fertilizer the problem of, be applied to after arable land, energy
A large amount of soil beneficial microbes are enough bred, there is suppression disease, growth promoting, make fertilizer, removing toxic substances and other effects；And can be by organic material decomposition
For organic nutriment in a fertilizer of plant available, precious Organic nutrient is provided for crop growth, strengthens fertilizer efficiency, promotes crop rapid
Growth.
Brief description of the drawings
Fig. 1 is the production technological process of solid-state biological organic fertilizer；
Fig. 2 is the structure chart of pair roller type cold extrusion organic granular fertilizer forming machine, and the mark in figure is：1st, hopper, 2, branch
Seat, 3, roller, 31, dome-type groove, 4, gear, 5, material-falling hopper.
Embodiment
Embodiment 1
A kind of solid-state biological organic fertilizer, its preparation method are as follows：
A. by after 3~6cm of crops stalk crushing, mixed with 30 times of straw decomposing inoculant of dilution, straw decomposing inoculant adds
Enter amount for stalk per ton add 4kg straw decomposing inoculants, build heap fermentation, fermentation temperature after 48 hrs, after reaching 60~65 DEG C, often
Once, water content is maintained at 50%~70%, and pH value is 6.5~7.5 for its turning；
B. after fermenting three days, N, P, K fertilizer are added, the addition of N, P, K fertilizer is：N fertilizer adds 3.5kg, P fertilizer for stalk per ton
1.5kg is added for stalk per ton, K fertilizer adds 0.2kg for stalk per ton, and uniformly mixing, continues to ferment；After tenth day, temperature is
30~40 DEG C, water content is 30%~50%, and once, after fermenting the 15th day, temperature is identical with room temperature, aqueous for every turning in two days
Measure as 15%, obtain the good stalk base-material of fermentation maturity.
C. the good stalk base-material of fermentation maturity is screened, a diameter of 2.5cm of mesh, in the base-material after screening, often
Ton uniformly participates in corn fine powder 40kg, N fertilizer 3kg, P fertilizer 1.5kg, K fertilizer 0.5kg, and mixed base-material water content is adjusted to
25%~35%, cold extrusion granulation is carried out, solid-state biological organic fertilizer is can obtain after drying.
The equipment that the cold extrusion is granulated is pair roller type cold extrusion organic granular fertilizer forming machine, two bearings；
Two rollers, roller both ends are installed in rotation on two bearings respectively, and two roller outer peripheral faces are in contact；
Roller surfaces are provided with a series of dome-type grooves, and in two roller relative rotational motions, two rollers are in contact
Outer peripheral face on dome-type groove it is corresponding formed a ball-type groove；
The roll shaft that two rollers respectively have one end stretches out bearing, is respectively fixed with a gear on roll shaft, and the two of two rollers
Individual gear is meshed；So that two rollers relatively rotate；
One roller is connected with power set；
Hopper, it is arranged on above roller, between two rollers of discharging opening face of hopper；
Also there is material-falling hopper, material-falling hopper is located at below roller, and the length of material-falling hopper is more than the length of roller；
The length of hopper is less than the length of roller；
Power set are motor, and the rotating shaft of motor and the roll shaft of roller connect；
Power set can also use link chain mechanism, can install sprocket wheel on the roll shaft of roller, be driven by link chain mechanism；
Bearing, hopper and material-falling hopper are each attached in mounting bracket.
The preparation method of the straw decomposing inoculant is：
(1) by bacillus subtilis, bacillus megaterium, trichoderma harzianum, trichoderma viride, bacillus thuringiensis point
It is not seeded on solid agar medium, 30 DEG C of culture 38h；
(2) strain after step (1) is cultivated, is inoculated in fluid nutrient medium respectively, cultivates；At 30 DEG C, shaking flask speed
60r/min, cultivate 38h；The Liquid Culture based component is：Sucrose 10g, glucose 10g, K2HPO40.2g, CaCO32g,
NaCl 0.4g, distilled water are settled to 1L；The culture medium of five kinds of bacterium is identical；
(3) strain after step (2) is cultivated is respectively placed in fermentation tank, and volume production culture bacterium number into zymotic fluid reaches 10
×1010Individual/ml；Fermentation medium components are：Glucose 40g/L, molasses 60g/L, urea 4g/L, KH2PO46g/L, remaining is
Distilled water；PH value 6.8；Volume production condition of culture is：Strain inoculum concentration is 5%；120h is cultivated at 30 DEG C, per 12h in incubation
5min is stirred, air 10min is filled per 2h, air mass flow is liquid per ton 2 cubic metres of inflation per minute.
(4) by the strain after step (3) volume production culture by bacillus subtilis 15%, bacillus megaterium 15%, Ha Ci
Trichoderma 25%, trichoderma viride 25%, the mixing of the ratio of bacillus thuringiensis 20%, intersect antagonism culture, used medium
Composition is：Glucose 20g/L, molasses 30g/L, urea 5g/L, P2O55g/L, KH2PO42g/L, remaining is distillation water ph value
6.8.Intersecting antagonism condition of culture is：Per 12h, stirring 5min, every 2h inflate 10min, co-culture 120h, be made in incubation
Straw decomposing inoculant.
In the greenhouse of plantation for many years, two pieces of ground are marked respectively as check plot and trial zone to plant tomato, check plot
For 1 mu, trial zone is 1.2 mu, and two pieces of ground impose 15-15-15 N P and K NPK 80kg/ mu conducts before the planting
Base fertilizer, which is turned over, to bury, and then check plot directly carries out tomato planting, and trial zone is uniformly made on arable land surface using the present embodiment
Ton/mu of solid-state biological organic fertilizer 3 after plant tomato again, other planting conditions all sames, experimental result is as shown in table 1：
Table 1
| Title | Area (mu) | Total output | Average product kg/ mus |
| Trial zone | 1.2 | 14695.6 | 12246.3 |
| Check plot | 1.0 | 7279.2 | 7279.2 |
It can be seen from the results above that trial zone increases production 68.2% than check plot, and during tomato growth, it is right
It is many according to dead of area's rice shoot early stage, and trial zone then not dead substantially, it is seen that solid-state biological organic fertilizer disclosed by the invention has
Volume increase, the effect of seedling-protection.
Embodiment 2
A kind of solid-state biological organic fertilizer, its preparation method are as follows：
A kind of solid-state biological organic fertilizer, its preparation method are as follows：
A. by after 3~6cm of crops stalk crushing, mixed with 30 times of straw decomposing inoculant of dilution, straw decomposing inoculant adds
Enter amount for stalk per ton add 5kg straw decomposing inoculants, build heap fermentation, fermentation temperature after 48 hrs, after reaching 60~65 DEG C, often
Once, water content is maintained at 50%~70%, and pH value is 6.5~7.5 for its turning；
B. after fermenting three days, N, P, K fertilizer are added, the addition of N, P, K fertilizer is：N fertilizer adds 5kg for stalk per ton, and P fertilizer is
Stalk per ton adds 1kg, and K fertilizer adds 0.2kg for stalk per ton, and uniformly mixing, continues to ferment；After tenth day, temperature be 30~
40 DEG C, water content is 30%~50%, and once, after fermenting the 15th day, temperature is identical with room temperature, and water content is for every turning in two days
15%, obtain the good stalk base-material of fermentation maturity.
C. the good stalk base-material of fermentation maturity is screened, a diameter of 2.5cm of mesh, in the base-material after screening, often
Ton uniformly participates in corn fine powder 40kg, N fertilizer 3kg, P fertilizer 1.5kg, K fertilizer 0.5kg, and mixed base-material water content is adjusted to
25%~35%, cold extrusion granulation is carried out, solid-state biological organic fertilizer is can obtain after drying.
The equipment that the cold extrusion is granulated is pair roller type cold extrusion organic granular fertilizer forming machine, two bearings；
Two rollers, roller both ends are installed in rotation on two bearings respectively, and two roller outer peripheral faces are in contact；
Roller surfaces are provided with a series of dome-type grooves, and in two roller relative rotational motions, two rollers are in contact
Outer peripheral face on dome-type groove it is corresponding formed a ball-type groove；
The roll shaft that two rollers respectively have one end stretches out bearing, is respectively fixed with a gear on roll shaft, and the two of two rollers
Individual gear is meshed；So that two rollers relatively rotate；
One roller is connected with power set；
Hopper, it is arranged on above roller, between two rollers of discharging opening face of hopper；
Also there is material-falling hopper, material-falling hopper is located at below roller, and the length of material-falling hopper is more than the length of roller；
The length of hopper is less than the length of roller；
Power set are motor, and the rotating shaft of motor and the roll shaft of roller connect；
Power set can also use link chain mechanism, can install sprocket wheel on the roll shaft of roller, be driven by link chain mechanism；
Bearing, hopper and material-falling hopper are each attached in mounting bracket.
The preparation method of the straw decomposing inoculant is：
(1) by bacillus subtilis, bacillus megaterium, trichoderma harzianum, trichoderma viride, bacillus thuringiensis point
It is not seeded on solid agar medium, 30 DEG C of culture 46h；
(2) strain after step (1) is cultivated, is inoculated in fluid nutrient medium respectively, cultivates；At 30 DEG C, shaking flask speed
60r/min, cultivate 46h；The Liquid Culture based component is：Sucrose 10g, glucose 10g, K2HPO40.2g, CaCO32g,
NaCl 0.4g, distilled water are settled to 1L；The culture medium of five kinds of bacterium is identical；
(3) strain after step (2) is cultivated is respectively placed in fermentation tank, and volume production culture bacterium number into zymotic fluid reaches 15
×1010Individual/ml；Fermentation medium components are：Glucose 50g/L, molasses 90g/L, urea 9g/L, KH2PO49g/L, remaining is
Distilled water；PH value 7.2；Volume production condition of culture is：Strain inoculum concentration is 7%；120h is cultivated at 30 DEG C, per 12h in incubation
5min is stirred, air 10min is filled per 2h, air mass flow is liquid per ton 2 cubic metres of inflation per minute.
(4) by the strain after step (3) volume production culture by bacillus subtilis 10%, bacillus megaterium 20%, Ha Ci
Trichoderma 20%, trichoderma viride 25%, the mixing of the ratio of bacillus thuringiensis 25%, intersect antagonism culture, used medium
Composition is：20~40g/L of glucose, 30~50g/L of molasses, urea 5~12g/L, P2O55~10g/L, KH2PO42~10g/
L, remaining is distillation water ph value 6.8~7.2.Intersecting antagonism condition of culture is：Per 12h, stirring 5min, every 2h fill in incubation
Gas 10min, 120h is co-cultured, straw decomposing inoculant is made.
In the greenhouse of plantation 6 years, two pieces of ground are marked respectively as check plot and trial zone to plant cucumber, check plot
It is 1.0 with trial zone, two pieces of ground impose 15-15-15 N P and K NPK 80kg/ mus as base fertilizer before the planting
Turn over and bury, then check plot directly carries out tomato planting, and trial zone is uniformly applied solid made from the present embodiment on arable land surface
Ton/mu of state biological organic fertilizer 3 plants cucumber again afterwards, other planting conditions all sames, and experimental result is as shown in table 2：
Table 2
| Title | Area (mu) | Total output | Average product kg/ mus |
| Trial zone | 1.0 | 12443.3 | 12443.3 |
| Check plot | 1.0 | 9987.6 | 9987.6 |
It can be seen from the results above that trial zone is than check plot volume increase 24.6%.
Above-mentioned reference embodiment is to a kind of straw decomposing inoculant and preparation method thereof and passes through the decomposed stalk of this decomposing agent in batches
Make the detailed description that the method for solid-state biological organic fertilizer is carried out, be it is illustrative rather than limited, can be according to being limited
Scope includes several embodiments, therefore changing and modifications in the case where not departing from present general inventive concept, should belong to the present invention's
Within protection domain.
Claims (10)
1. a kind of straw decomposing agent, it is characterised in that the straw decomposing inoculant includes the raw material of following percentage by weight：Withered grass bud
Spore bacillus 10~15%, bacillus megaterium 15~20%, trichoderma harzianum 20~25%, trichoderma viride 20~25%, Soviet Union
Cloud gold bacillus 15~25%.
2. the preparation method of straw decomposing inoculant according to claim 1, it is characterised in that the preparation method includes following
Step：
(1) bacillus subtilis, bacillus megaterium, trichoderma harzianum, trichoderma viride, bacillus thuringiensis are connect respectively
Kind is on solid agar medium, culture；
(2) strain after step (1) is cultivated, is inoculated in fluid nutrient medium respectively, cultivates；
(3) strain after step (2) is cultivated is respectively placed in fermentation tank, and volume production culture bacterium number into zymotic fluid reaches 10~15
×1010Individual/ml；
(4) strain after step (3) volume production culture is mixed by formula ratio, intersects antagonism culture, straw decomposing inoculant is made.
3. preparation method according to claim 2, it is characterised in that in the step (3), fermentation medium components are：
30~60g/L of glucose, 50~100g/L of molasses, urea 1~10g/L, KH2PO45~10g/L, remaining is distilled water；PH is
6.8~7.2.
4. the preparation method according to Claims 2 or 3, it is characterised in that in the step (4), intersect antagonism culture, institute
It is with medium component：20~40g/L of glucose, 30~50g/L of molasses, urea 5~12g/L, P2O55~10g/L, KH2PO4
2~10g/L, remaining is distilled water, and pH is 6.8~7.2.
5. the preparation method according to Claims 2 or 3, it is characterised in that the condition of culture in the step (3) and (4)
For：120h is cultivated at 30 DEG C, 5min is stirred per 12h in incubation, fills air 10min per 2h.
A kind of 6. preparation method of solid-state biological organic fertilizer, it is characterised in that：The preparation method comprises the following steps：
A. by 30 times of straw decomposing dilution agent described in claim 1, mixed with the stalk after crushing, build heap and ferment three days；
B. N, P, K fertilizer are added, continues to ferment, obtains the good stalk base-material of fermentation maturity；
C. the good stalk base-material of fermentation maturity is screened through screen cloth, after incorporation cornstarch, N fertilizer, P fertilizer and K fertilizer, adjustment system contains
Water is 25%~35%, and the solid-state biological organic fertilizer is can obtain after cold extrusion is granulated, dried.
7. preparation method according to claim 6, it is characterised in that in the step a, the addition of straw decomposing inoculant is
The 0.3~0.5% of stalk weight, the condition for building heap fermentation are：Fermentation temperature after 48 hrs, after reaching 60~65 DEG C, daily
Once, water content is maintained at 50%~70%, and pH value is 6.5~7.5, is fermented three days for turning.
8. the preparation method according to claim 6 or 7, it is characterised in that in the step b, N, P, K fertilizer in stalk per ton
Addition be：0.2~0.5kg of N fertilizer 2~5kg, P fertilizer 0.5~1.5kg, K fertilizer；Fermentation condition is：After tenth day, temperature 30
~40 DEG C, water content is 30%~50%, and once, after fermenting the 15th day, temperature is identical with room temperature, water content for every turning in two days
For 15%.
9. the preparation method according to claim 6 or 7, it is characterised in that in the step c, in stalk base-material per ton
Even incorporation corn fine powder 40kg, N fertilizer 3kg, P fertilizer 1.5kg, K fertilizer 0.5kg.
10. the solid-state biological organic fertilizer that preparation method according to claim 6 is prepared.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108863462A (en) * | 2018-08-09 | 2018-11-23 | 安徽瑞驰兰德生物科技有限公司 | It is a kind of using stalk as biological organic fertilizer of raw material and preparation method thereof |
| CN110041133A (en) * | 2019-05-07 | 2019-07-23 | 哈尔滨禾野农业有限公司 | A kind of bacteria agent and preparation method for straw decomposing |
| CN110699289A (en) * | 2019-10-31 | 2020-01-17 | 潍坊科技学院 | Preparation method and application of straw degradation composite microbial inoculum |
| CN112913369A (en) * | 2021-01-22 | 2021-06-08 | 东北农业大学 | Method for preventing and controlling alkalization of sunshine greenhouse planting soil salt |
| CN113264801A (en) * | 2021-06-22 | 2021-08-17 | 江苏富寿康农业生物技术有限公司 | Preparation method of bio-organic fertilizer |
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| CN105385637A (en) * | 2015-12-17 | 2016-03-09 | 青州伟格生物科技有限公司 | Functional microorganism decomposing agent and preparation method and application thereof |
| CN106916771A (en) * | 2017-04-25 | 2017-07-04 | 安徽瑞驰兰德生物科技有限公司 | A kind of straw decomposing inoculant and using this kind of straw decomposing inoculant realize agricultural crop straw year after year continuous cropping full dose on the spot also field method |
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| CN105385637A (en) * | 2015-12-17 | 2016-03-09 | 青州伟格生物科技有限公司 | Functional microorganism decomposing agent and preparation method and application thereof |
| CN106916771A (en) * | 2017-04-25 | 2017-07-04 | 安徽瑞驰兰德生物科技有限公司 | A kind of straw decomposing inoculant and using this kind of straw decomposing inoculant realize agricultural crop straw year after year continuous cropping full dose on the spot also field method |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108863462A (en) * | 2018-08-09 | 2018-11-23 | 安徽瑞驰兰德生物科技有限公司 | It is a kind of using stalk as biological organic fertilizer of raw material and preparation method thereof |
| CN110041133A (en) * | 2019-05-07 | 2019-07-23 | 哈尔滨禾野农业有限公司 | A kind of bacteria agent and preparation method for straw decomposing |
| CN110699289A (en) * | 2019-10-31 | 2020-01-17 | 潍坊科技学院 | Preparation method and application of straw degradation composite microbial inoculum |
| CN112913369A (en) * | 2021-01-22 | 2021-06-08 | 东北农业大学 | Method for preventing and controlling alkalization of sunshine greenhouse planting soil salt |
| CN113264801A (en) * | 2021-06-22 | 2021-08-17 | 江苏富寿康农业生物技术有限公司 | Preparation method of bio-organic fertilizer |
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