CN1073341C - Spring heading chinese cabbage cross-transformation method - Google Patents
Spring heading chinese cabbage cross-transformation method Download PDFInfo
- Publication number
- CN1073341C CN1073341C CN96115692A CN96115692A CN1073341C CN 1073341 C CN1073341 C CN 1073341C CN 96115692 A CN96115692 A CN 96115692A CN 96115692 A CN96115692 A CN 96115692A CN 1073341 C CN1073341 C CN 1073341C
- Authority
- CN
- China
- Prior art keywords
- hybridization
- species
- spring
- selfing
- seed
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Pretreatment Of Seeds And Plants (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Cultivation Of Plants (AREA)
Abstract
The present invention relates to a hybridization and transformation method for spring heading Chinese cabbages, which is used for cultivating the production seeds of hybridization Chinese cabbages with high quality in spring. In the method, two species with complementation of disease resistance and economic character are selected; through continuous purification for three to four years, high-generation selfing lines are sieved out and are respectively used as parents for hybridization to generate hybrid species in one generation; the hybrid species carry out autocopulation in single plant for two years to generate selfing species in the secondary generation; after hybridization with different lines, the selfing species in the secondary generation are cultivated into quality species with high quality. The breeding method has the advantages of short period, convenient method and easy operation; the cultured species has disease resistance, high yield and good spring cultivation adaptability; the method of the present invention is a tried and true seed production method.
Description
The present invention relates to a kind of hybridization transfer method of spring Chinese cabbage.
Spring, the breeding objective of Chinese cabbage mainly was viral diseases, double downy mildew resistance and soft rot, late bolting, kipeming high yield etc., mostly its breeding method is directly to utilize the cabbage hybrid of landrace or external import, according to the continuous selfing of breeding objective, the pedigree selection such as ecological and field resistance in conjunction with spring, behind 5 generations above purifying, carry out combining ability test, sow in spring and ecologically identify that meeting breeding objective requirement rear can be applicable to produce.The spring Chinese cabbage selects for use used breeding material mostly to be the number of sheets type of filling the span of a man's arms at present, these material economic characters are good, bolting evening, but equal viral diseases not, under China mainland sex climate condition, sow in spring or all high susceptible viral disease of sowing in early autumn, diseased plant rate is more than 90%, can have no harvest when serious, so utilize continuous selfed breeding spring Chinese cabbage kind can not solve antiviral problem.Moreover, utilize the ecological screening of sowing in spring often to need spring and educate a large amount of young plants, cause workload to strengthen, it is easily mashed to be faced with the maternal plant of selecting and remain simultaneously, is difficult for a difficult problem of collecting seed.Disease resistance screening only depends on the qualification result of artificial infection, often with the land for growing field crops multiple diseases or infect situation with the complexity of a plurality of strains systems of a kind of disease (bion) and be not inconsistent.Separately carry out if identify in artificial infection and field, often cause selection inaccurate, waste the labour, even can seriously lose the high material of some breedings value.
The object of the invention is intended to utilize two fens pure lines of disease resistance and economic characters complementation to hybridize transformation, thereby turns out the breeding method of the good disease-resistant a kind of spring Chinese cabbage of economic characters.
The inventive method is as follows: select the king-pile of antiviral double downy mildew resistance, folded armful crossbreed for use, through continuous 3 years individual plant selfing, filter out high the type pure lines of filling the span of a man's arms and be female parent; Filtering out the type pure lines of filling the span of a man's arms with continuous 4 years selfing purifying of packet header, landrace Fushan, Shandong lotus is male parent, sow in the Winter-Spring, carry out artificial hybridization in the flower bud phase in spring, receive behind the seed in autumn sowing then, the select and remain during results maternal plant of first cross, crop is colonizated in the greenhouse after staying root, and room temperature is at 10～28 ℃, the flower bud phase is carried out individual plant selfing, and single list of receiving is hidden.This seed is grown seedlings in 1 year planted in fall, adopt after the artificial inoculation on seedling virus field planting land for growing field crops and the directly two kinds of methods evaluations in field planting land for growing field crops (the impression virus naturally) disease resistances of back of growing seedlings again, select disease-resistant strain harvest time, crop stays root to be colonizated in the greenhouse, room temperature keeps 10～28 ℃, select disease-resistant strain to carry out individual plant selfing in the flower bud phase, adopt behind the seed in the 3rd year planted in fall in the land for growing field crops, the disease-resistant strain of selecting and remain after the Chinese cabbage maturation, crop stays root to be colonizated in the greenhouse, room temperature is 10～28 ℃, and the flower bud phase is carried out the hybridization of different system, receipts seed, and this seed is the breeding of cultivation.
Narrate below in conjunction with accompanying drawing.
Fig. 1 is this transfer method flow chart, wherein 1 is the folded armful crossbreed of king-pile, select this kind pure lines 2,3rd after three generations's selfing, Fushan Bao Toulian filters out pure lines 4 through the selfing of four generations, to be sheerly 2 is female parent, pure lines 4 are carried out the hybridization of flower bud phase in spring for male parent, cultivate first generation cross-fertilize seed 5, and crossbreed 5 is in autumn sowing then, the maternal plant of selecting and remain when gathering in the crops, crop is colonizated in the greenhouse after staying root, and the flower bud phase is carried out individual plant selfing, thereby produces generation selfed seed 6, selfed seed 6 is grown seedlings in autumn sowing in 1 year, through filtering out disease-resistant strain behind impression or the virus inoculation naturally, harvest time, crop stayed root to be colonizated in the greenhouse, selected the disease-resistant strain flower bud phase to carry out individual plant selfing, cultivate two generation selfed seed 7, two generation selfed seed 7 autumn sowing in the 3rd year, ripe back crop stays root, is colonizated in the greenhouse, the flower bud phase is carried out the hybridization of different system, cultivates hybridization Chinese cabbage breeding 8.
The inventive method owing to select maternal tool viral diseases and downy mildew male parent select bolting evening, the good kind of economic characters to hybridize transformation, make that breeding cycle is short, method is easy, easy to operate, utilize the kind viral diseases and the downy mildew of this method seed selection, it is good to fit planting property in high yield, spring, real Chinese cabbage improved seeds for sowing in spring under the China mainland sex climate condition.
Claims (1)
1. the hybridization transfer method of a spring Chinese cabbage is characterized in that selecting for use the king-pile of antiviral double downy mildew resistance, folded armful crossbreed, through continuous 3 years individual plant selfing, filters out high the type pure lines of filling the span of a man's arms and is female parent; Select the selfing in continuous 4 years of packet header, Fushan lotus to filter out to fill the span of a man's arms the type pure lines to be male parent, sow in the Winter-Spring, the flower bud phase is carried out artificial hybridization, receive behind the seed in autumn sowing then, the select and remain during results maternal plant of first cross, crop is colonizated in the greenhouse after staying root, and room temperature is at 10～28 ℃, and the flower bud phase is carried out individual plant selfing, this kind is grown seedlings in 1 year planted in fall after producing seed, the two kinds of methods in the direct field planting land for growing field crops in the field planting land for growing field crops and the back of growing seedlings are identified disease resistance after adopting artificial inoculation on seedling virus, and disease-resistant strain is selected in ripe back, and crop stays root to be colonizated in the greenhouse, room temperature is at 10～28 ℃, select disease-resistant strain to carry out individual plant selfing in the flower bud phase, the 3rd year planted in fall selects disease-resistant strain in big Tanaka behind the receipts seed after the maturation, crop stays root to be colonizated in the greenhouse, room temperature is 10～28 ℃, and the flower bud phase is carried out the hybridization of different system, adopts seed after setting seeds.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN96115692A CN1073341C (en) | 1996-03-15 | 1996-03-15 | Spring heading chinese cabbage cross-transformation method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN96115692A CN1073341C (en) | 1996-03-15 | 1996-03-15 | Spring heading chinese cabbage cross-transformation method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1140012A CN1140012A (en) | 1997-01-15 |
| CN1073341C true CN1073341C (en) | 2001-10-24 |
Family
ID=5122918
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN96115692A Expired - Fee Related CN1073341C (en) | 1996-03-15 | 1996-03-15 | Spring heading chinese cabbage cross-transformation method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1073341C (en) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102172210B (en) * | 2011-02-24 | 2013-03-13 | 山东春秋大白菜育种研究中心 | Breeding method for high-resistance TuMV late bolting yellow core spring cabbages |
| CN102172211B (en) * | 2011-02-24 | 2013-03-20 | 山东春秋大白菜育种研究中心 | Multi-resistant high-quality Qingmaye type red-orange-core Chinese cabbage breeding method |
| CN107787836A (en) * | 2016-08-31 | 2018-03-13 | 辽宁东亚农业发展有限公司 | A kind of spring Chinese Cabbage material system breeding technique |
| CN110972931A (en) * | 2019-12-17 | 2020-04-10 | 山西省农业科学院蔬菜研究所 | Breeding method of disease-resistant bolting-resistant spring Chinese cabbage |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1070790A (en) * | 1992-09-21 | 1993-04-14 | 山西省农业科学院蔬菜研究所 | The method of Chinese cabbage crossing breeding |
| CN1094216A (en) * | 1993-04-28 | 1994-11-02 | 天津市蔬菜研究所 | A kind of method of green pepper hybrid seeding |
-
1996
- 1996-03-15 CN CN96115692A patent/CN1073341C/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1070790A (en) * | 1992-09-21 | 1993-04-14 | 山西省农业科学院蔬菜研究所 | The method of Chinese cabbage crossing breeding |
| CN1094216A (en) * | 1993-04-28 | 1994-11-02 | 天津市蔬菜研究所 | A kind of method of green pepper hybrid seeding |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1140012A (en) | 1997-01-15 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN108849474B (en) | Method for improving yield of sweet potato group hybrid seed production | |
| CN105475121B (en) | A kind of selection of high temperature resistant disease resisting rice two-line sterile line | |
| CN100337533C (en) | Transferring and seed producing method for head cabbage cytoplasm male sterile line | |
| CN102217526B (en) | A kind of selection of black barley | |
| CN110073968A (en) | A kind of fast breeding method of high oily salt tolerant peanut | |
| CN103004583B (en) | Rapid purification method for cucumber female flower lines | |
| CN105379615A (en) | Wheat blue and abortive sterile germplasm and breeding method thereof | |
| CN103461101B (en) | Stemless cucurbita maxima breeding method | |
| CN108077061A (en) | A kind of selection made wine with the glutinous two-line sterile lines of Xian | |
| CN103299896A (en) | Culturing method of eurytropic bolting-resisting spring Chinese cabbage free microspores | |
| CN107360966A (en) | Xian round-grained rice osculant sterile line spring river 35A early when a kind of colored, stigma exposing ratio is high selection and propagation method | |
| CN101124886B (en) | Hybrid seed production method using cotton sterile male line and recovery line to processing ratoon regeneration | |
| CN105284591A (en) | Breeding method for limited fruit branch type short-season cotton suitable for being planted in Yangtze River valley cotton areas | |
| CN1073341C (en) | Spring heading chinese cabbage cross-transformation method | |
| CN100353833C (en) | Method for breeding seeds of hybridized tomato | |
| CN110100722B (en) | Breeding method of purple-leaf white-flower cabbage type landscape rape conventional variety | |
| CN102668975A (en) | Method for producing seed by using three-line hybrid rice sterile line with purple leaf marker | |
| CN1019939C (en) | Seed production method of sunflower oil seed' Neihuaza II | |
| CN112616651B (en) | Breeding method of glyphosate-resistant cotton genic male sterile dual-purpose line | |
| CN103733982B (en) | A kind of selection of tomato parent pure lines of anti-tomato late blight | |
| CN1647615A (en) | Method for breeding, reproducing and preparing hybrid seeds of cytoplasmic male sterile line of flowering Chinese cabbage | |
| CN107410005A (en) | A kind of spinach cenospecies and its selection | |
| CN103798125A (en) | Method for acquiring novel species of brassicaceous vegetables and application method of novel specie of brassicaceous vegetables | |
| CN112640776A (en) | Breeding of rice photo-thermo sensitive sterile line with high outcrossing rate and seed production method thereof | |
| CN111165343A (en) | Breeding method of dual-purpose genic male sterile line with high outcrossing rate and high combining ability |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C06 | Publication | ||
| PB01 | Publication | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C19 | Lapse of patent right due to non-payment of the annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |