CN107320586A - It is a kind of to treat traditional Chinese medicine injection of hog cholera and preparation method thereof - Google Patents
It is a kind of to treat traditional Chinese medicine injection of hog cholera and preparation method thereof Download PDFInfo
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- A61K36/28—Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
- A61K36/282—Artemisia, e.g. wormwood or sagebrush
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- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/42—Cucurbitaceae (Cucumber family)
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
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- A61K9/0019—Injectable compositions; Intramuscular, intravenous, arterial, subcutaneous administration; Compositions to be administered through the skin in an invasive manner
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- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
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Abstract
The invention provides a kind of traditional Chinese medicine injection for treating swine fever and preparation method thereof, the traditional Chinese medicine injection is by weight, it is made up of 3 25 parts of sweet wormwood, 3 25 parts of mushroom, 5 15 parts of rattletop, 5 25 parts of short-tube lycoris, 5 25 parts of balsam pear, it is used to treat hog cholera effect highly significant, and rapid-action, the features such as being very suitable for hog cholera rapid onset, propagate fast.
Description
Technical field
The invention belongs to technical field of traditional Chinese medicines, and in particular to traditional Chinese medicine injection for the treatment of hog cholera and preparation method thereof.
Background technology
Hog cholera be caused by the CSFV (Hogcholera virus, Swine fever virus), harm pig and
Wild boar, other animals do not fall ill.Swine fever is a kind of acute, hot, highly contagious disease, is mainly characterized by high temperature, micro- blood
Pipe is denatured and causes systemic bleeding, necrosis, infraction.Swine fever endangers extremely serious to pig, can cause pig industry heavy losses.At present
Swine fever prevalence usually shows irregular provincialism and distributed, and pathogeneticing characteristic is not true to type, and with heating, spiritual depressed, anorexia, coughs
Cough, incoordination, conjunctivitis and diarrhoea, perinatal death, stillborn foetus, miscarriage.Medium virulence and strong poison are infected with the above
Symptom, and the fast propagation of all ages in swinery.
Effective medicine is there is no at present, makes a definite diagnosis and takes measures in time in early days, to controlling and eliminating swine fever, reduce economy
Loss is significant.Traditional prevention and controls use to pig body and inject An Jibilin, quinine, pacify injection greatly, but it is chemistry
Medicine, does not meet the requirement of green non-pollution pig.
The content of the invention
There is provided a kind of Chinese medicine traditional Chinese medicine injection for treating swine fever and its preparation side in order to solve the above problems by the present invention
Method, the Chinese medicine composition is made up of sweet wormwood, mushroom, rattletop, short-tube lycoris, balsam pear, and it is used to treat hog cholera effect highly significant, and
And rapid-action, the features such as being very suitable for hog cholera rapid onset, propagate fast.
Specifically, a kind of traditional Chinese medicine injection for treating swine fever, by weight, by 3-25 parts of sweet wormwood, 3-25 parts of mushroom, rises
Numb 5-15 parts, 5-25 parts of short-tube lycoris, 5-25 parts of balsam pear are made.
Further, a kind of traditional Chinese medicine injection for treating swine fever, by weight, by 8-20 parts of sweet wormwood, 8-20 parts of mushroom,
8-12 parts of rattletop, 10-20 parts of short-tube lycoris, 10-20 parts of compositions of balsam pear.
Yet further, a kind of traditional Chinese medicine injection for treating swine fever, by weight, by 14 parts of sweet wormwood, 14 parts of mushroom, rises
10 parts of fiber crops, 15 parts of short-tube lycoris, 15 parts of balsam pear are made.
Present invention also offers a kind of method for the traditional Chinese medicine injection for treating swine fever, comprise the following steps：
(1) sweet wormwood, mushroom, rattletop, short-tube lycoris, balsam pear are crushed, plus 1-3h is boiled in 3-5 times of decocting, is filtered, filtrate concentration, is done
It is dry, extract powder is made, the dregs of a decoction are standby.
(2) step (1) dregs of a decoction are taken to add 60-80% alcohol reflux to extract 40-60min, filtering, filtrate recycling ethanol is dense
Contracting, dries, obtains medicinal powder.
(3) step (1) and step (2) plus 8-10 times are measured into the dissolving of deionized water heating stirring, placed after cooling, it is excessively inorganic
Purpose ceramic-film filter, membrane aperture is 25nm, reclaims filtrate, and 120 DEG C of sterilizing 10-15min are fitted into ampoule bottle, every 10ml, i.e.,
Obtain Chinese medicine composition traditional Chinese medicine injection of the present invention.
Step (1) described above is to take sweet wormwood, mushroom, rattletop, short-tube lycoris, balsam pear to crush, plus 2h is boiled in 3 times of decoctings, is filtered,
Filtrate concentrates, and dries, and extract powder is made, and the dregs of a decoction are standby.
The alcohol reflux that step (2) described above takes step (1) dregs of a decoction to add 70% extracts 50min, and filtering, filtrate is reclaimed
Ethanol, is concentrated, and is dried, is obtained medicinal powder.
(3) dissolve step (1) and step (2) plus 9 times of amount deionized water heating stirrings the step of institute on above-mentioned, place cold
But after, inorganic ceramic film filter is crossed, membrane aperture is 25nm, reclaims filtrate, 120 DEG C of sterilizing 10-15min are fitted into ampoule bottle,
Every 10ml, that is, obtain Chinese medicine composition traditional Chinese medicine injection of the present invention.
Beneficial effects of the present invention are：
Sweet wormwood：Hardship, it is pungent, tremble with fear.Return liver, gallbladder channel, three burnt, kidney channels.Clear abnormal heat, cool blood is except steaming, except steaming, preventing malaria.For heat
Heresy heating, fever due to yin deficiency, night fever abating at dawn, hectic fever due to yin labor heat, malaria fever and chills, jaundice with damp-heat pathogen.
Mushroom：Modern medicine study finds that it contains a variety of antiviral ingredients, the inducing agent double-strand ribose core of such as interferon
Acid.
Rattletop：【Meridian distribution of property and flavor】Pungent, micro-sweet, is slightly cold.Return lung, spleen, stomach, large intestine channel.【Major function】Deliver promoting eruption, heat-clearing
Removing toxic substances, rises and lifts yang-energy.For headache due to pathogenic wind-heat, dentalgia, aphtha, abscess of throat, measles without adequate eruption, sun poison hair spot；Prolapse of the anus, uterus takes off
Hang down.
Balsam pear：【Nature and flavor】It is cold, it is bitter；【Function】Clearing away summerheat, improving eyesight removing toxic substances.
Short-tube lycoris：Meridian distribution of property and flavor】Acrid, sweet, warm.It is poisonous.【Major function】Detumescence, desinsection.
The present invention carries out prescription using sweet wormwood, mushroom, rattletop, short-tube lycoris, balsam pear traditional Chinese medicine of the five flavours material, and each medicine complements each other, and assists
Same synergy, plays solve strongly fragrant poison altogether, the effect of filling blood, for treating hog cholera effect highly significant, and of the invention by Chinese traditional medicine composition
Thing prepares parenteral solution, and its is rapid-action, and cure rate is very suitable for hog cholera up to more than 83%, and morbidity is unexpected, infects fast feature control
System, and it is uneasy to recur and without any side effects, use safety.
To further illustrate present disclosure and beneficial effect, the present invention has also listed following test example, it is intended to illustrate
The present invention, but never protection scope of the present invention is limited in any way.
1st, the recipe determination research of Chinese medicine composition of the present invention：
Experimental animal：The big white mouse (120 ± 4g) of Pass Test standard is taken, 200, is infected with CSFV (CSFV) big
White mouse, will appear from lassitude, and the blue color of auricle, the successful big white mouse of modeling takes out 140, is randomly divided into 7 groups, every group 20
Only, treated with following test group prescription drug, test result analysis are carried out after 4 days.
Test group 1：Using the parenteral solution being made up of sweet wormwood 3g, mushroom 3g, rattletop 5g, short-tube lycoris 5g, balsam pear 5g.
Test group 2：Using the parenteral solution being made up of sweet wormwood 8g, mushroom 8g, rattletop 8g, short-tube lycoris 10g, balsam pear 10g.
Test group 3：Using the parenteral solution being made up of sweet wormwood 14g, mushroom 14g, rattletop 10g, short-tube lycoris 15g, balsam pear 15g.
Test group 4：Using the parenteral solution being made up of sweet wormwood 20g, mushroom 20g, rattletop 12g, short-tube lycoris 20g, balsam pear 20g.
Test group 5：Using the parenteral solution being made up of sweet wormwood 25g, mushroom 25g, rattletop 15g, short-tube lycoris 25g, balsam pear 25g groups.
Test group 6：Using the parenteral solution being made up of sweet wormwood 14g, mushroom 14g, rattletop 10g, balsam pear 15g.
Test group 7：Using the parenteral solution being made up of mushroom 14g, rattletop 10g, short-tube lycoris 15g, balsam pear 15g.
Blank control group：Do not give any pharmaceutical intervention.
Equal conditions free water diet during experiment, test group 1-7 injects 5ml/kg to big white mouse daily, is united after 4 days
Count treatment, result of the test such as table 1 below：
Result of the test shows, test group 1-7 to treatment big white mouse CSFV (CSFV) metainfective therapeutic effect with it is right
Compare according to group, be respectively provided with significant curative effect, and test group 1-5 is significantly better than test group 6-7 groups, therefore preferred therapeutic pig of the present invention
The Chinese medicine composition of pest is：By 3-25 parts of sweet wormwood, 3-25 parts of mushroom, 5-15 parts of rattletop, 5-25 parts of short-tube lycoris, 5-25 parts of systems of balsam pear
Into.More it is made up of choosing 8-20 parts of sweet wormwood, 8-20 parts of mushroom, 8-12 parts of rattletop, 10-20 parts of short-tube lycoris, 10-20 parts of balsam pear.It is optimal
Elect as：It is made up of 14 parts of sweet wormwood, 14 parts of mushroom, 10 parts of rattletop, 15 parts of short-tube lycoris, 15 parts of balsam pear.Treatment swine fever produced by the present invention
Traditional Chinese medicine injection clinical tests：
In the swine fever epidemic disease that in March, 2017 occurs, two groups are divided into 30 sick pigs and is treated, every group 15, examination
Test group：To wanting parenteral solution produced by the present invention to be treated, injection volume is 10ml/50kg, once a day, and successive administration 4 days is right
According to group 2：Injected and treated using florfenicol injection, successive administration 4 days carries out result of the test investigation, wherein, test group treatment
The effective percentage of swine fever sick pig is up to 85%, and control group treats the efficient up to 87% of swine fever sick pig.Illustrate in the obtained Chinese medicine of the present invention
Medicine parenteral solution has significant curative effect to hog cholera, suitable with florfenicol injection effect.
2nd, Chinese medicine composition Toxicity test of the present invention：
Take testing standard mouse (19 ± 1.2g) 40, be randomly divided into 2 groups, test group give injection it is produced by the present invention in
Drug composition parenteral solution, injection volume is 8ml/kg, is administered once daily, successive administration 7 days, control group：Any medicine is not awarded to do
In advance, two groups of equal conditions carry out drinking water diet, and as a result continuous 15 days observation mouse show, two groups of mouse survival behaviors, activity and
Drinking water diet, without significant difference, is normal.Illustrate safety non-toxic of the present invention, to pig without obvious injury.
With embodiment：
Embodiment 1：The preparation method of traditional Chinese medicine injection of the present invention：
Formula：Sweet wormwood 3g, mushroom 3g, rattletop 5g, short-tube lycoris 5g, balsam pear 5g；
Preparation method：
(1) sweet wormwood, mushroom, rattletop, short-tube lycoris, balsam pear are crushed, plus 1h is boiled in 3 times of decoctings, is filtered, filtrate concentration, is dried, system
Extract powder is obtained, the dregs of a decoction are standby.
(2) alcohol reflux for taking step (1) dregs of a decoction to add 60% extracts 40min, and filtering, filtrate recycling ethanol is concentrated, done
It is dry, obtain medicinal powder.
(3) step (1) and step (2) plus 8 times of amount deionized water heating stirrings are dissolved, placed after cooling, excessively inorganic pottery
Porcelain film filter, membrane aperture is 25nm, reclaims filtrate, and 120 DEG C of sterilizing 10min are fitted into ampoule bottle, every 10ml obtains
Chinese medicine composition traditional Chinese medicine injection of the present invention.
Embodiment 2：The preparation method of traditional Chinese medicine injection of the present invention：
Formula：Sweet wormwood 8g, mushroom 8g, rattletop 8g, short-tube lycoris 10g, balsam pear 10g；
Preparation method：
(1) sweet wormwood, mushroom, rattletop, short-tube lycoris, balsam pear are crushed, plus 2.5h is boiled in 4 times of decoctings, is filtered, filtrate concentration, is dried,
Extract powder is made, the dregs of a decoction are standby.
(2) alcohol reflux for taking step (1) dregs of a decoction to add 70% extracts 50min, and filtering, filtrate recycling ethanol is concentrated, done
It is dry, obtain medicinal powder.
(3) step (1) and step (2) plus 9 times of amount deionized water heating stirrings are dissolved, placed after cooling, excessively inorganic pottery
Porcelain film filter, membrane aperture is 25nm, reclaims filtrate, and 120 DEG C of sterilizing 12min are fitted into ampoule bottle, every 10ml obtains
Chinese medicine composition traditional Chinese medicine injection of the present invention.
Embodiment 3：The preparation method of traditional Chinese medicine injection of the present invention
Formula：Sweet wormwood 14g, mushroom 14g, rattletop 10g, short-tube lycoris 15g, balsam pear 15g；
Preparation method：(1) sweet wormwood, mushroom, rattletop, short-tube lycoris, balsam pear are crushed, plus 2.5h is boiled in 4 times of decoctings, is filtered, filtrate
Concentration, is dried, and extract powder is made, and the dregs of a decoction are standby.
(2) alcohol reflux for taking step (1) dregs of a decoction to add 70% extracts 50min, and filtering, filtrate recycling ethanol is concentrated, done
It is dry, obtain medicinal powder.
(3) step (1) and step (2) plus 9 times of amount deionized water heating stirrings are dissolved, placed after cooling, excessively inorganic pottery
Porcelain film filter, membrane aperture is 25nm, reclaims filtrate, and 120 DEG C of sterilizing 12min are fitted into ampoule bottle, every 10ml obtains
Chinese medicine composition traditional Chinese medicine injection of the present invention.
Embodiment 4：The preparation method of traditional Chinese medicine injection of the present invention
Formula：Sweet wormwood 20g, mushroom 20g, rattletop 12g, short-tube lycoris 20g, balsam pear 20g；
Preparation method：(1) sweet wormwood, mushroom, rattletop, short-tube lycoris, balsam pear are crushed, plus 3h is boiled in 5 times of decoctings, is filtered, filtrate is dense
Contracting, is dried, and extract powder is made, and the dregs of a decoction are standby.
(2) alcohol reflux for taking step (1) dregs of a decoction to add 80% extracts 60min, and filtering, filtrate recycling ethanol is concentrated, done
It is dry, obtain medicinal powder.
(3) step (1) and step (2) plus 10 times of amount deionized water heating stirrings are dissolved, placed after cooling, excessively inorganic pottery
Porcelain film filter, membrane aperture is 25nm, reclaims filtrate, and 120 DEG C of sterilizing 15min are fitted into ampoule bottle, every 10ml obtains
Chinese medicine composition traditional Chinese medicine injection of the present invention.
Test group 5：The preparation method of Chinese medicine composition of the present invention
Formula：Sweet wormwood 25g, mushroom 25g, rattletop 15g, short-tube lycoris 25g, balsam pear 25g；
Preparation method：(1) sweet wormwood, mushroom, rattletop, short-tube lycoris, balsam pear are crushed, plus 3h is boiled in 5 times of decoctings, is filtered, filtrate is dense
Contracting, is dried, and extract powder is made, and the dregs of a decoction are standby.
(2) alcohol reflux for taking step (1) dregs of a decoction to add 80% extracts 60min, and filtering, filtrate recycling ethanol is concentrated, done
It is dry, obtain medicinal powder.
(3) step (1) and step (2) plus 10 times of amount deionized water heating stirrings are dissolved, placed after cooling, excessively inorganic pottery
Porcelain film filter, membrane aperture is 25nm, reclaims filtrate, and 120 DEG C of sterilizing 15min are fitted into ampoule bottle, every 10ml obtains
Chinese medicine composition traditional Chinese medicine injection of the present invention.
Claims (7)
1. a kind of traditional Chinese medicine injection for treating swine fever, it is characterised in that：By weight, by 3-25 parts of sweet wormwood, 3-25 parts of mushroom,
5-15 parts of rattletop, 5-25 parts of short-tube lycoris, 5-25 parts of balsam pear are made.
2. the traditional Chinese medicine injection for the treatment of swine fever according to claim 1, it is characterised in that：By weight, by sweet wormwood 8-
20 parts, 8-20 parts of mushroom, 8-12 parts of rattletop, 10-20 parts of short-tube lycoris, 10-20 parts of balsam pear composition.
3. the traditional Chinese medicine injection for the treatment of swine fever according to claim 1 or 2, it is characterised in that：By weight, by sweet wormwood
14 parts, 14 parts of mushroom, 10 parts of rattletop, 15 parts of short-tube lycoris, 15 parts of balsam pear be made.
4. a kind of preparation method for the traditional Chinese medicine injection for treating swine fever, it is characterised in that：Comprise the following steps：
(1) sweet wormwood, mushroom, rattletop, short-tube lycoris, balsam pear are crushed, plus 1-3h is boiled in 3-5 times of decocting, is filtered, filtrate concentration, is dried, system
Extract powder is obtained, the dregs of a decoction are standby.
(2) step (1) dregs of a decoction are taken to add 60-80% alcohol reflux to extract 40-60min, filtering, filtrate recycling ethanol is concentrated, done
It is dry, obtain medicinal powder.
(3) step (1) and step (2) plus 8-10 times are measured into the dissolving of deionized water heating stirring, placed after cooling, cross inorganic ceramic
Film filter, membrane aperture is 25nm, reclaims filtrate, and 120 DEG C of sterilizing 10-15min are fitted into ampoule bottle, every 10ml obtains
Chinese medicine composition traditional Chinese medicine injection of the present invention.
5. the preparation method of the traditional Chinese medicine injection for the treatment of swine fever according to claim 4, it is characterised in that：Step (1) is
Sweet wormwood, mushroom, rattletop, short-tube lycoris, balsam pear is taken to crush, plus 2h is boiled in 3 times of decoctings, is filtered, filtrate concentration, dries, extract powder, medicine is made
Slag is standby.
6. the preparation method of the traditional Chinese medicine injection for the treatment of swine fever according to claim 4, it is characterised in that：Step (2) is
The alcohol reflux for taking step (1) dregs of a decoction to add 70% extracts 50min, and filtering, filtrate recycling ethanol concentrates, dries, obtain medicinal powder.
7. the preparation method of the traditional Chinese medicine injection for the treatment of swine fever according to claim 4, it is characterised in that：Institute the step of
(3) it is, by step (1) and step (2) plus 9 times of amount deionized water heating stirring dissolvings, to place after cooling, cross inorganic ceramic membrane mistake
Filter, membrane aperture is 25nm, reclaims filtrate, and 120 DEG C of sterilizing 10-15min are fitted into ampoule bottle, every 10ml obtains this hair
Bright Chinese medicine composition traditional Chinese medicine injection.
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| CN201710644051.1A CN107320586A (en) | 2017-07-31 | 2017-07-31 | It is a kind of to treat traditional Chinese medicine injection of hog cholera and preparation method thereof |
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| CN201710644051.1A CN107320586A (en) | 2017-07-31 | 2017-07-31 | It is a kind of to treat traditional Chinese medicine injection of hog cholera and preparation method thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107233493A (en) * | 2017-06-29 | 2017-10-10 | 贞丰县食源特种养殖有限公司 | It is a kind of to treat traditional Chinese medicine injection of checken pest and preparation method thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105616879A (en) * | 2016-02-19 | 2016-06-01 | 廖冼飞 | Composition for treating hog cholera |
| CN107137485A (en) * | 2017-06-29 | 2017-09-08 | 石阡县金桥农牧发展有限责任公司 | It is a kind of to treat traditional Chinese medicine injection of swine fever and preparation method thereof |
| CN107137444A (en) * | 2017-05-31 | 2017-09-08 | 贵州省铜仁市万山区京军生态农牧发展有限公司 | It is a kind of to treat Chinese medicine composition of swine fever and preparation method thereof |
-
2017
- 2017-07-31 CN CN201710644051.1A patent/CN107320586A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105616879A (en) * | 2016-02-19 | 2016-06-01 | 廖冼飞 | Composition for treating hog cholera |
| CN107137444A (en) * | 2017-05-31 | 2017-09-08 | 贵州省铜仁市万山区京军生态农牧发展有限公司 | It is a kind of to treat Chinese medicine composition of swine fever and preparation method thereof |
| CN107137485A (en) * | 2017-06-29 | 2017-09-08 | 石阡县金桥农牧发展有限责任公司 | It is a kind of to treat traditional Chinese medicine injection of swine fever and preparation method thereof |
Non-Patent Citations (1)
| Title |
|---|
| 李江生: "《现代养殖实用技术》", 31 July 2005, 贵州人民出版社 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107233493A (en) * | 2017-06-29 | 2017-10-10 | 贞丰县食源特种养殖有限公司 | It is a kind of to treat traditional Chinese medicine injection of checken pest and preparation method thereof |
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