CN107236670A - A kind of dendrobium candidum Tissue Culture Flask - Google Patents
A kind of dendrobium candidum Tissue Culture Flask Download PDFInfo
- Publication number
- CN107236670A CN107236670A CN201710627124.6A CN201710627124A CN107236670A CN 107236670 A CN107236670 A CN 107236670A CN 201710627124 A CN201710627124 A CN 201710627124A CN 107236670 A CN107236670 A CN 107236670A
- Authority
- CN
- China
- Prior art keywords
- bottle cap
- bottleneck
- horizontal segment
- tissue culture
- main body
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
- 241000026010 Dendrobium candidum Species 0.000 title claims abstract description 13
- 229920001971 elastomer Polymers 0.000 claims abstract description 15
- 239000011521 glass Substances 0.000 claims abstract description 5
- 239000002131 composite material Substances 0.000 claims abstract description 4
- 238000010422 painting Methods 0.000 claims abstract description 4
- VGGSQFUCUMXWEO-UHFFFAOYSA-N Ethene Chemical compound C=C VGGSQFUCUMXWEO-UHFFFAOYSA-N 0.000 claims description 2
- 229920006389 polyphenyl polymer Polymers 0.000 claims 1
- 239000004793 Polystyrene Substances 0.000 abstract description 3
- 229920002223 polystyrene Polymers 0.000 abstract description 3
- 230000000694 effects Effects 0.000 abstract description 2
- 238000004113 cell culture Methods 0.000 description 7
- 235000015097 nutrients Nutrition 0.000 description 7
- 239000003921 oil Substances 0.000 description 5
- 230000010261 cell growth Effects 0.000 description 3
- 230000012010 growth Effects 0.000 description 3
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000011534 incubation Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 235000012771 pancakes Nutrition 0.000 description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 229910002092 carbon dioxide Inorganic materials 0.000 description 1
- 239000001569 carbon dioxide Substances 0.000 description 1
- 230000003833 cell viability Effects 0.000 description 1
- 230000019522 cellular metabolic process Effects 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 229920002379 silicone rubber Polymers 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/08—Flask, bottle or test tube
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/04—Filters; Permeable or porous membranes or plates, e.g. dialysis
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
- C12M37/02—Filters
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Clinical Laboratory Science (AREA)
- Molecular Biology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a kind of dendrobium candidum Tissue Culture Flask, including bottle cap, pellicle, main body, horizontal segment and rubber layer, the main body is by bottle cap, body is constituted with horizontal segment, and body is arranged on the lower section of bottle cap, bottleneck is provided between body and bottle cap, the horizontal segment and main body are formed integrally using the composites such as polystyrene or glass, and horizontal segment is arranged on the side of bottom part body, bottleneck is provided with above the bottleneck, and the tangent plane size of bottleneck and bottle cap is coincide, handle is provided with the top of the bottle cap, multiple air-vents are carved with the outer wall of bottle cap, and pellicle is provided with bottle cap, the left and right ends of the pellicle are provided with adhesion piece, and rubber layer is circularized, it is fixed on by adhesion on the tangent plane at the top of the tangent plane of the bottom of bottle cap and bottleneck, contact surface between the rubber layer is provided with painting oil reservoir.The present invention has good air permeable function, and cell propagation is fast, and culture effect is good, in extensive range.
Description
Technical field
The present invention relates to cell culture vessel equipment technical field, specially a kind of dendrobium candidum Tissue Culture Flask.
Background technology
Cell culture refers under conditions of manually providing, the growth that cell is carried out in vitro, in incubation
The cell of growth is no longer divided into tissue, is the basic and important means of production in biotechnology.Its purpose is to harvest
Cell in itself or obtain products of cellular metabolism.Tissue Culture Flask provides necessary environment for the growth of cell, prior art
Blake bottle poor air permeability, is unfavorable for cell growth, meanwhile, required in cell cultivation process to the cell cultivated every
Certain time is observed once, after the blake bottle culture cell in existing research, Bioexperiment teaching and clinical medicine experiment, needs
Nutrient solution is taken out and is placed on slide, puts and observes under the microscope, easily the nutrient solution containing cell in pollution blake bottle,
And troublesome poeration, add the work difficulty of experimenter.
According in patent 201120546838.2, existing blake bottle is easily spilt in operating or handling process, it is impossible to solved both
Need the problem of exchange of air is effectively anti-spillage again, it is impossible to meet the demand of people.
The content of the invention
It is an object of the invention to provide a kind of dendrobium candidum Tissue Culture Flask, to solve what is proposed in above-mentioned background technology
Problem.
To achieve the above object, the present invention provides following technical scheme：A kind of dendrobium candidum Tissue Culture Flask, including bottle
Lid, pellicle, main body, horizontal segment and rubber layer, the main body are made up of bottle cap, body and horizontal segment, and body is arranged on bottle
The lower section of lid, is provided with bottleneck, the horizontal segment and main body are using composite or glass such as polystyrene between body and bottle cap
Glass is formed integrally, and horizontal segment is arranged on above the side of bottom part body, the bottleneck and is provided with bottleneck, and bottleneck and bottle cap
Tangent plane size coincide, be provided with the top of the bottle cap on handle, the outer wall of bottle cap and be carved with multiple air-vents, and set in bottle cap
Pellicle is equipped with, the left and right ends of the pellicle are provided with adhesion piece, and rubber layer is circularized, and bottle cap is fixed on by adhesion
Bottom tangent plane and bottleneck at the top of tangent plane on, the contact surface between the rubber layer is provided with painting oil reservoir.
It is preferred that, the main body is shaped as cylinder or cuboid.
It is preferred that, the air-vent is shaped as circular port or square opening.
It is preferred that, both the bottle cap and handle are formed integrally.
It is preferred that, the bottom face of the horizontal segment is flushed with the bottom surface of body.
Compared with prior art, the beneficial effects of the invention are as follows：
(1) pellicle is provided with the bottle cap of the blake bottle, with good air permeable function, Bottle cap top is provided with
Multiple air-vents, are conducive to cell to take a breath, and meet the demand of oxygen and carbon dioxide, are conducive to cell growth, and cell propagation is fast,
Culture effect is good；
(2) when needing observation cell, the horizontal segment for being shaped as pancake cuboid is placed under microscope, can directly be observed
The cell cultivated, without the nutrient solution containing cell is taken out, then is positioned over observation on slide, effectively avoids dirt
The nutrient solution in blake bottle is contaminated, it is simple to operate, it is time saving and energy saving, mitigate the work difficulty of experimenter,
(3) while the pellicle of the present invention can effectively prevent external microbe from entering pollution-free in blake bottle, bottle, cell is made
Growth, breed at one's best, cell viability is high.
(4) present invention has wide range of applications, and is applicable not only to scientific research cell culture, school's biology curriculum experiment cell
Culture, is also applied for clinical medicine experiment cell culture.
(5) the culture bottle cap uses rubber ring with bottleneck intersection, is provided with and applies the sealing that oil reservoir ensure that bottle mouth position
Property, it is to avoid the blake bottle situation that nutrient solution is spilt in operating or handling process, reduce germ contamination or experimental data occurs
The possibility of mistake.
Brief description of the drawings
Fig. 1 is structural representation of the invention；
Fig. 2 is the bottleneck attachment structure sectional view of bottle cap and bottle in the present invention.
In figure：1- shakes hands；2- bottle caps；3- pellicles；4- bottlenecks；5- main bodys；6- bodies；7- horizontal segments；8- air-vents；9-
Adhesion piece；10- rubber layers；11- applies oil reservoir；12- bottlenecks.
Embodiment
Below in conjunction with the accompanying drawing in the embodiment of the present invention, the technical scheme in the embodiment of the present invention is carried out clear, complete
Site preparation is described, it is clear that described embodiment is only a part of embodiment of the invention, rather than whole embodiments.It is based on
Embodiment in the present invention, it is every other that those of ordinary skill in the art are obtained under the premise of creative work is not made
Embodiment, belongs to the scope of protection of the invention.
Refer to Fig. 1 and Fig. 2, a kind of embodiment that the present invention is provided：A kind of dendrobium candidum Tissue Culture Flask, including bottle cap
2nd, pellicle 3, main body 5, horizontal segment 7 and rubber layer 10, main body 5 are made up of bottle cap 2, body 5 and horizontal segment 7, and body 5 is set
Bottleneck 4 is provided between the lower section of bottle cap 2, body 5 and bottle cap 2, horizontal segment 7 and main body 5 are using composite woods such as polystyrene
Material or glass are formed integrally, and horizontal segment 7 is arranged on the side of the bottom of main body 5, and the top of bottleneck 4 is provided with bottleneck 12, and bottle
The tangent plane size of mouth 12 and bottle cap 2 is coincide, and the top of bottle cap 2, which is provided with handle 1, the outer wall of bottle cap 2, is carved with multiple air-vents
Pellicle 3 is provided with 8, and bottle cap 2, the left and right ends of pellicle 3 are provided with adhesion piece 9, and pellicle 3 can use polytetrafluoro
The medical macromolecular materials such as ethene or silicon rubber are made, and rubber layer 10 is circularized, and the bottom of bottle cap 2 is fixed on by adhesion
On the tangent plane at the top of tangent plane and bottleneck 12, the contact surface between rubber layer 10 is provided with painting oil reservoir 11, and main body 5 is shaped as cylinder
Body or cuboid, air-vent 8 are shaped as circular port or square opening, and pellicle 3 is fixed on the inwall of bottle cap 2 by adhesion piece 9
On, bottle cap 2 is formed integrally with both handles 1, and the bottom face of horizontal segment 7 is flushed with the bottom surface of body 5.
Operation principle：In use, nutrient solution is added in blake bottle, pellicle 3 is fixed on bottle cap 2 by adhesion piece 9
On, blake bottle is interior to form one with ventilative cell culture environment, bottle cap 2 and the rubber layer 10 of the contact surface setting of bottleneck 12
With applying oil reservoir 11, the bottleneck 12 of blake bottle is fully sealed, do not result in needs to observe cell in nutrient solution leakage, incubation
When, the horizontal segment 7 for being shaped as pancake cuboid is placed in micro- Microscopic observation.
It is obvious to a person skilled in the art that the invention is not restricted to the details of above-mentioned one exemplary embodiment, Er Qie
In the case of without departing substantially from spirit or essential attributes of the invention, the present invention can be realized in other specific forms.Therefore, no matter
From the point of view of which point, embodiment all should be regarded as exemplary, and be nonrestrictive, the scope of the present invention is by appended power
Profit is required rather than described above is limited, it is intended that all in the implication and scope of the equivalency of claim by falling
Change is included in the present invention.Any reference in claim should not be considered as to the claim involved by limitation.
Claims (6)
1. a kind of dendrobium candidum Tissue Culture Flask, including bottle cap (2), pellicle (3), main body (5), horizontal segment (7) and rubber layer
(10), it is characterised in that：The main body (5) is made up of bottle cap (2), body (5) and horizontal segment (7), and body (5) is arranged on bottle
The lower section of (2) is covered, bottleneck (4) is provided between body (5) and bottle cap (2), the horizontal segment (7) and main body (5) use polyphenyl
The composites such as ethene or glass are formed integrally, and horizontal segment (7) is arranged on the side of main body (5) bottom, the bottleneck (4)
Top is provided with bottleneck (12), and the tangent plane size of bottleneck (12) and bottle cap (2) is coincide, and is provided with the top of the bottle cap (2)
Shake hands (1), be carved with the outer wall of bottle cap (2) in multiple air-vents (8), and bottle cap (2) and be provided with pellicle (3), it is described semi-transparent
The left and right ends of film (3) are provided with adhesion piece (9), and rubber layer (10) is circularized, and the bottom of bottle cap (2) is fixed on by adhesion
Tangent plane and bottleneck (12) at the top of tangent plane on, the contact surface between the rubber layer (10) is provided with painting oil reservoir (11).
2. a kind of dendrobium candidum Tissue Culture Flask according to claim 1, it is characterised in that：The shape of the main body (5)
For cylinder or cuboid.
3. a kind of dendrobium candidum Tissue Culture Flask according to claim 1, it is characterised in that：The shape of the air-vent (8)
Shape is circular port or square opening.
4. a kind of dendrobium candidum Tissue Culture Flask according to claim 1, it is characterised in that：The pellicle (3) passes through
Adhesion piece (9) is fixed on the inwall of bottle cap (2).
5. a kind of dendrobium candidum Tissue Culture Flask according to claim 1, it is characterised in that：The bottle cap (2) and handle
(1) the two is formed integrally.
6. a kind of dendrobium candidum Tissue Culture Flask according to claim 1, it is characterised in that：The bottom of the horizontal segment (7)
End face is flushed with the bottom surface of body (5).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710627124.6A CN107236670A (en) | 2017-07-28 | 2017-07-28 | A kind of dendrobium candidum Tissue Culture Flask |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710627124.6A CN107236670A (en) | 2017-07-28 | 2017-07-28 | A kind of dendrobium candidum Tissue Culture Flask |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107236670A true CN107236670A (en) | 2017-10-10 |
Family
ID=59988368
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710627124.6A Withdrawn CN107236670A (en) | 2017-07-28 | 2017-07-28 | A kind of dendrobium candidum Tissue Culture Flask |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107236670A (en) |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN202369595U (en) * | 2011-12-23 | 2012-08-08 | 哈尔滨商业大学 | Cell culture flask |
| CN205576165U (en) * | 2016-04-01 | 2016-09-14 | 斯坦姆（天津）细胞工程技术有限公司 | Modified cell culture bottle |
| CN205874410U (en) * | 2016-08-02 | 2017-01-11 | 唐璟鸿 | Culture dish lid with ventilation function |
| CN206219609U (en) * | 2016-11-24 | 2017-06-06 | 齐齐哈尔医学院 | Tissue cultuer bottle |
| CN206352145U (en) * | 2016-12-27 | 2017-07-25 | 范嘉琦 | A kind of Bioexperiment organized enzyme culture dish |
-
2017
- 2017-07-28 CN CN201710627124.6A patent/CN107236670A/en not_active Withdrawn
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN202369595U (en) * | 2011-12-23 | 2012-08-08 | 哈尔滨商业大学 | Cell culture flask |
| CN205576165U (en) * | 2016-04-01 | 2016-09-14 | 斯坦姆（天津）细胞工程技术有限公司 | Modified cell culture bottle |
| CN205874410U (en) * | 2016-08-02 | 2017-01-11 | 唐璟鸿 | Culture dish lid with ventilation function |
| CN206219609U (en) * | 2016-11-24 | 2017-06-06 | 齐齐哈尔医学院 | Tissue cultuer bottle |
| CN206352145U (en) * | 2016-12-27 | 2017-07-25 | 范嘉琦 | A kind of Bioexperiment organized enzyme culture dish |
Non-Patent Citations (1)
| Title |
|---|
| 李秀霞等: "《生物学实验指导下》", 31 October 2014, 东北大学出版社 * |
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|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WW01 | Invention patent application withdrawn after publication | ||
| WW01 | Invention patent application withdrawn after publication |
Application publication date: 20171010 |