CN107223774A - A kind of many effect yeast for animal feeds albumen powders and its application - Google Patents
A kind of many effect yeast for animal feeds albumen powders and its application Download PDFInfo
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- CN107223774A CN107223774A CN201710677808.7A CN201710677808A CN107223774A CN 107223774 A CN107223774 A CN 107223774A CN 201710677808 A CN201710677808 A CN 201710677808A CN 107223774 A CN107223774 A CN 107223774A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
- A23K20/147—Polymeric derivatives, e.g. peptides or proteins
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/163—Sugars; Polysaccharides
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- Proteomics, Peptides & Aminoacids (AREA)
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Abstract
The invention discloses a kind of many effect yeast for animal feeds albumen powders and its application, it is that the mixing Aspergillus and 4% saccharomycete for adding its weight 2% by fermentation materials and respectively are formulated, and the fermentation materials are prepared from by agricultural byproducts, oil plant miscellaneous dregs of rice, keratin；The mixing Aspergillus is by by 3:The black-koji mould and aspergillus oryzae of 1 ratio are mixed；It can be widely used in cultivation and feed manufacturing.
Description
Technical field
The present invention relates to cultivation industry field, more particularly to a kind of many effect yeast for animal feeds albumen powders and its application.
Background technology
The protein raw material that current aquaculture is used is divided into two kinds, and one kind is animal protein, most representational to be
Fish meal.Another is vegetable protein, and what is most represented is dregs of beans.Other miscellaneous dregs of rice for example cotton dregs, rapeseed meal, also have brewage byproduct
The protein content such as grain, slag>20% also uses as protein raw material.But anti-nutrition is there is in the use of these raw materials
The factor and nutrition inhibin.
1st, the inhibin of enzyme
Cyclopropane fatty acid present in cotton dregs and gossypol are all nutritional autagonism composition, the bag that wherein cyclopropane fatty acid includes
Certain herbaceous plants with big flowers acid and SEMEN STERCULIAE LYCHNOPHORAE acid are the inhibin of enzyme, are the materials of anti-unsaturationization enzyme.This inhibin can make saturation in livestock and poultry blood
Aliphatic acid is dominant, is body fat hardening, is the hard crisp and yolk hardening of the white of an egg of laying hen.And make the rate of fertilization of kind of ovum and incubate
Rate is reduced.
2nd, vitamin inhibitor
Yan acid inhibin, the cereal such as corn, wheat, sorghum be cultivation with main smart grain, quantitative determined through chemical method, typically all
Containing 1-3% milligram of Yan acid.But, the feeding of such a native state not active Yan acid little through the effective Yan acid of Biology identification survey in animal body
Contain the sour inhibin of Yan in grain.Dermatitis is suffered from due to a lack of Yan acid with corn, wheat, the breeding stock of sorghum feeding and kind fowl.
3rd, mineral feed inhibitor
3.1st, phytic acid is the inhibin of the salt such as calcium, magnesium, zinc, iron
It is present in the plant feeds such as grain, wheat bran, rice bran, oil cake phytic acid, accounts for the 70% of total phosphorus content, wherein about 40-
90% is phytate phosphorus.Phytic acid turns into phytic acid magnesium, calcium, iron, zinc etc. in plant and is difficult to dissolving with reference to salt.In the diet of cultivation
Calcium, magnesium, iron, the zinc that middle phytic acid is supplemented in nonruminant alimentary canal and diet are combined into phytic acid calcium, magnesium, iron, zinc salt i.e. original shape
Excrete and be not absorbed and used, mineral deficiency easily occur.
3.2nd, oxalic acid is calcium salt inhibin
Oxalic acid content highest is sesame-send cake, and other grouts and seed also have different content.Digestion of the oxalic acid in nonruminant
Combined and precipitated with calcium in road, and original shape is excreted, and hinders absorbing for calcium.
3.2nd, sour glucoside is that thyroid gland swashs inhibin
Sour glucoside is present in Erucic acid, and rape cauline leaf and oilseeds grain in growth contain sulphur glucoside, in the presence of itself sulphur glucoside enzyme,
Produce isothiocyanates（It is commonly called as mustard oil）He Liu oxazolidones, it plays the role of suppression thyroid hormone , oxazolidones and can also
Cause Thyroid Gland Swell.
The problem of existing more than now mends influence of the various additives reduction to livestock and poultry beyond being all, virtually add feeding
Cost is fed, improper addition can also influence the quality and residual of meat, jeopardize the health of people.Aquaculture lacks one kind and can be used as albumen
Matter is using can eliminate the feedstuff of inhibin in feed again.
The content of the invention
The present invention seeks to provided for above-mentioned the deficiencies in the prior art a kind of many effect yeast for animal feeds albumen powders and its
Using.
The present invention realizes that above-mentioned purpose is adopted the technical scheme that：A kind of many effect yeast for animal feeds albumen powders, it be by
Fermentation materials and add the mixing Aspergillus and 4% saccharomycete of its weight 2% respectively and be formulated, the fermentation materials be by
The raw material of following parts by weight is prepared from：8-9 parts of agricultural byproducts, oil plant miscellaneous dregs of rice 9-16,26-32 parts of keratin：The mixing
Aspergillus is by by 3:The black-koji mould and aspergillus oryzae of 1 ratio are mixed.
Further, three kinds of the black-koji mould can produce carbohydrase respectively and production pectase, xylan be mould, cellulose
The black-koji mould of enzyme and phytase generating presses 1:1:1 ratio is mixed.
Further, the saccharomycete is candida utili bacterium.
Further, the agricultural byproducts are any one in corn germ cake, rice bran meal, pomace, jujube ground-slag, corn steep liquor
Kind.
Further, the oil plant miscellaneous dregs of rice is any one in the cotton seed dregs of rice, the vegetable seeds dregs of rice, palm kernel meal, the ribes dregs of rice, alcohol slag
Kind.
A kind of application of above-mentioned many effect yeast for animal feeds albumen powders in cultivation and feed manufacturing.
The present invention principle and beneficial effect be：The inhibin of feedstuff is disappeared in cud livestock cultivates in cultivation
Remove, because itself has biological fermentation process.So using microbial solid fermentation method, cheap, not comprehensive nutrition is produced again
Nutritional autagonism and the raw material that is not utilized directly by nonruminant is balanced proportioning by the amino acid structure of dregs of beans, through microorganism
Decomposition and inversion is many effect Yeast proteins.The shortage of protein can be made up for livestock and poultry cultivation and solves itself institute
The enzyme system of shortage, eliminates the nutritional autagonism problem brought in feed.The bacterium protein of fermented gained is used for aquaculture and feeding
Material processing industry can play a variety of effectiveness.1. Partial Fish Meal and dregs of beans, are substituted as protein feed, it has the activity as fish meal
The factor-UGF, Substitution for Soybean Meal and the anti-nutrition component without trypsase.It compensate for the shortage of feed protein matter.
2., the thalline of mycoprotein consolidates cellulase with a grain of salt, pectase, carbohydrase, amylase, protease, phytase.Thalline
Application of the albumen in cultivation and feed manufacturing, without adding enzyme preparation again, can play the effect for surmounting addition.A variety of exogenous enzymes
Supplement eliminate anti-nutrient substance in feed, improve feed digestibility 3., mycoprotein enter animal gastrointestinal tract part viable bacteria
Body can produce organic acid, and it can voluntarily adjust animal digestive system acid-base value.The addition for eliminating acidulant is used.4., disappear
Solve mycotoxin：Multiple-effect mycoprotein can produce a certain amount of aspergillus polysaccharide when the object bacteria that production process is used acts on material
And zymosan.Mannosan especially in yeast cell wall is referred to as new adsorbent.A variety of moulds in its energy adsorption feed
Toxin.Mycotoxin is small molecular organic compounds, is not complicated protein molecule.It is organic contained by multiple-effect mycoprotein
Acid tool inoxidizability, to organic matter ionization property and the hydroxylation solution toxicity of Toxic, Green Tea Extract, anti-inflammatory, liver-protective function
Acted synergistically with polysaccharide and more cleared up the harm of mycotoxin.5., suppress scatol and improve meat quality：The generation of scatol
It is that diet and endogenous protein are produced under livestock and poultry caecum and the effect of large intestine anaerobe, especially harmful Escherichia coli,
The L-Trp formation indole-3-acetic acid decarboxylation that can degrade of the corruption ammonia-producing bacterium such as clostridium, proteus forms scatol.
Multiple-effect mycoprotein is added in feed and constantly supplements active bacteria and a variety of enzyme systems for livestock and poultry, forms surging competing in enteron aisle with spoilage organisms
Strive, supplement bacterium inhibits the living space and condition of scatol producing strains with beneficial probiotics in same environment holding high activity,
The generating source of scatol has been rooted out, the deposition in meat has been eliminated, improves meat quality and local flavor.6. livestock and poultry, are improved disease-resistant
Ability：Enteron aisle Escherichia coli, staphylococcus aureus, salmonella are killed in zymosan and the suppression of aspergillus polysaccharide, mediate the intestines of generation
Toxin；Both polysaccharide have raising livestock and poultry non-specific（Do not belong to vaccine）Property immunization, it is ripe to accelerate immunity function, Neng Gouxian
Write the disease-resistant and emergency capability that ground improves body.Its inoxidizability, effectively prevention animal are encroached on due to free radical, immunologic function
The a variety of difficult and complicated cases for lowly waiting and causing.Its various active has the effect of similar antibiotic without developing immunity to drugs, it
Health care prevention can be made in feed with substitute antibiotics to use.7., multiple-effect mycoprotein is rich in B family vitamin, this vitamin
Synthetic vitamin effectiveness than outer addition is high.Multiple-effect mycoprotein, which is added in feed, can make trace element play maximum effect
Shortage is made up with, it is to avoid Use out of range.8. breeding environment is improved：When the multiple-effect mycoprotein of addition 8% in feed, excrement row
Go out pH value ﹤ 7, at this moment the release of ammonia is seldom.Scatol is suppressed and produced in livestock and poultry body, and Tiny ecosystem is adjusted in livestock and poultry body in addition
Ammonia-producing bacterium is substituted afterwards, and the ammonia micro-ecological bacterial that do not produce of breeding environment forms advantage group, is persistently kept in breeding environment,
Excrement is not required to chemicals dissipation fly maggot also so that can not breed, and the ecological environment of a benign cycle promotes aquaculture sustainable
Development.
Embodiment
The present invention is further described below with reference to embodiment.
Many effect yeast for animal feeds albumen powders of the present invention, it is the mixing for adding its weight 2% by fermentation materials and respectively
Aspergillus and 4% saccharomycete are formulated, the fermentation materials be by agricultural byproducts, oil plant miscellaneous dregs of rice, keratin prepare and
Into：The mixing Aspergillus is by by 3:The black-koji mould and aspergillus oryzae of 1 ratio are mixed.
Further, three kinds of the black-koji mould can produce carbohydrase respectively and production pectase, xylan be mould, cellulose
The black-koji mould of enzyme and phytase generating presses 1:1:1 ratio is mixed.
Further, the saccharomycete is candida utili bacterium.
Further, the agricultural byproducts are any one in corn germ cake, rice bran meal, pomace, jujube ground-slag, corn steep liquor
Kind.
Further, the oil plant miscellaneous dregs of rice is any one in the cotton seed dregs of rice, the vegetable seeds dregs of rice, palm kernel meal, the ribes dregs of rice
Strain is selected and the production of hybrid seeds flow that spreads cultivation：
First, strain selects Chinese microorganism strain collection
1st, aspergillus niger：Produce carbohydrase bi0-66524
2nd, aspergillus niger：Produce that pectase, xylan be mould and cellulase bi0-68022
3rd, aspergillus niger：Phytase generating bi0-68008
4th, aspergillus oryzae：Produce protease bi0-66754
5th, candida utili：BNCC340012
2nd, strain spreads cultivation
1st, spread cultivation Nutrient medium
NaNO3 0.3%
K cl 0.05%
K2HPO4 0.1%
Fe SO4 0.001%
Mg SO4 0.005%
Sucrose 3%
2 grams of agar
100 milliliters of PH6.7 of water
Sterilizing is dissolved during above nutritional agents is discharged water, in gnotobasis to be assigned to sterile petri dish by nutrient solution while hot, after solidification
Test tube bacterium is respectively connected to culture dish, mark is performed, 35 DEG C of incubator to bacterium colonies is put and is covered with full ware.The another case culture of starter bacterium.
2nd, triangular flask spreads cultivation bacterium
500 milliliters of triangular flasks are taken, required wheat bran medium is weighed, with water 1:1 mixes wheat bran thoroughly, weighs what is mixed per triangular flask
20 grams of wheat bran, is covered with multilayer gauze and tied, autoclaving 1 hour.Opened after sterilizing to sterile room and take out triangular flask, each bottle is used
Inoculation shovel connects the strain in corresponding culture dish, marks.The another case culture of saccharomycete, puts 35 DEG C of incubator cultures 12 hours,
Wheat bran medium has been covered with white hypha, is broken up culture medium with aseptic inoculation hook or inoculation rake, as postponed length without being covered with mycelia
It is full to break up again.Bottle is sealed after breaing up to be further cultured for 12 hours, bacterium thunder and spore occurs in mycelium, continue to cultivate ripe to spore.
Saccharomycete culture to culture medium is all white, and it is standby that 4 DEG C of safes are put in termination culture.
3rd, spreading cultivation with bacterium is produced
3.1st, required wheat bran is weighed, with water 1:1 mixes wheat bran thoroughly, puts high-pressure sterilizing pot sterilizing.It is 0 that air pressure, which falls after rise, after sterilizing, to nothing
Bacterium room is opened, and is spread out into 30 × 45 centimeters of sterile disks with net bottom, and wheat bran is thick 3 centimeters.30 DEG C of environment temperature, 90 degree of humidity is black
Each one bottle of the bacterium of Aspergillus 3 kinds of different enzyme systems of often disk access.
3.2nd, often disk accesses 3 bottles of strains to aspergillus oryzae.
3.3rd, often disk accesses 4 bottles of strains to saccharomycete, and another strain room the same terms are individually cultivated.
12 hours mycelia are cultivated after inoculation and are covered with culture medium, caking is broken up, 24 hours mycelia of culture are covered with culture medium, have
A small amount of bacterium thunder occurs.
3.4th, microzyme culture medium is penetrating for white thalline.
The cultured wettable using fresh herb of strain, also can it is drying after use.
4th, strain collocation ratio
4.1st, the black-koji mould 75% of 3 kinds of mixed culture, aspergillus oryzae 25%.
4.2nd, Aspergillus and the proportioning of saccharomycete, mixing mould 1 and the ratio collocation of saccharomycete 4 are used.
3rd, the use matching principle of fermentation base-material
Use the keratin after the agricultural byproducts having no toxic side effect, oil plant miscellaneous dregs of rice, processing（Feather meal or wool powder）, by hair
Ferment material dry weight, wet fresh bacterium mixing Aspergillus 2%, saccharomycete 4%.Bacterium is distributed in the aqueous solution for mix material spice together
Inoculation.After assay, with the index balance fermentation bed material of the albumen and amino acid of dregs of beans or fish meal.Albumen after fermentation is
43-47%, total amino acid content 38-42%, rich in a variety of biology enzymes.
Raw material therein and it act as：Agricultural byproducts are corn germ cake, rice bran meal, pomace, jujube ground-slag, corn processing shallow lake
The byproduct corn steep liquor of powder.The mycotoxin that this kind of raw material is directly used in cultivation, raw material can endanger livestock and poultry.Phytic acid in raw material
The absorption of phosphorus, pectin, coarse-fibred ANFs influence livestock and poultry to nutrition.
Byproduct after Oil processing is that there is anti-battalion in the cotton seed dregs of rice, the vegetable seeds dregs of rice, palm kernel meal, the ribes dregs of rice, these raw materials
The factor is supported, these ANFs, which are not eliminated, can produce murder by poisoning, can also influence the utilization of nutritional ingredient.The anti-nutrition of cotton dregs because
Son：Gossypol, cyclopropene fatty acid, crude fibre；The ANFs of rapeseed meal：Glucosinolate, sinapine, tannin, phytic acid；Tea
The ANFs of the dregs of rice：Tea Saponin, tannin, the ANFs of palm kernel meal, β-mannosan
Cyokeratin：Feather meal, curing skin and flesh powder.This proteinoid is very high, but it has stable cystine linkage, can it is difficult to be decomposed into
The nutrients utilized.It passes through physical treatment, then the protein for being changed into easily utilizing through microorganism decomposition.
The above raw material has a variety of unfavorable factors as feedstuff, and nutrition is again uneven, even with necessary
The problem of controlling in a small amount of scope, but itself exist does not eliminate.
Nutritional ingredient of the above different material with reference to Soybean Meal, select several different materials to carry out nutrient balance, approach
With the albumen beyond Soybean Meal, it is balanced with ten kinds of amino acid before Soybean Meal, is that its different material balance is approached and beyond ammonia
The state of base acid.It is powerful using Aspergillus by microbial action in these basestocks in the case where such raw material is selected and is balanced
It is mould system decompose bed material, by Candida with mould decomposition using its analyte be itself thalline amount reproduction grow, most
It is that bed material is converted through microorganism decomposition eventually, eliminates toxin and ANFs in raw material, balanced as nutrition, enzyme system is enriched
Vegetable protein and Yeast protein, be cheap and the raw material resources of toxic side effect are converted into the protein of high-quality.
Fermentating formula example：
4th, application and consumption
8% is added in the processing of livestock and poultry complete diet pellet, without adding enzyme preparation, without adding antibiotic, without adding acidulant, without
Excess adds copper agent and other trace elements.Fish meal can 100% substitute fish meal, instead of dregs of beans 50% to 100%.According to granulation
Situation adjusts consumption.
The basic material formula of fermentation：
Fang Yi
Formula uses corn steep liquor 15Kg above, admixes material, required strain is added in water stirs evenly according to quantity, enters fermentation vat by water 17Kg
Ferment 24h, and material is covered with albicans Saccharomyces thalline, goes out pond drying, obtains multienzyme system yeast albumen powder.（Protein and amino acid are total
Number is that the content in raw material multiplies weight addition except gross weight）
Side two
Formula uses corn steep liquor 15Kg above, admixes material, required strain is added in water stirs evenly according to quantity, enters fermentation vat by water 17Kg
Ferment 24h, and material is covered with albicans Saccharomyces thalline, goes out pond drying, obtains multienzyme system yeast albumen powder.（Protein and amino acid are total
Number is that the content in raw material multiplies weight addition except gross weight）
Side three
Formula uses corn steep liquor 15Kg above, admixes material, required strain is added in water stirs evenly according to quantity, enters fermentation vat by water 17Kg
Ferment 24h, and material is covered with albicans Saccharomyces thalline, goes out pond drying, obtains multienzyme system yeast albumen powder.（Protein and amino acid are total
Number is that the content in raw material multiplies weight addition except gross weight）.
Claims (6)
1. a kind of many effect yeast for animal feeds albumen powders, it is characterised in that：It is by fermentation materials and adds its weight 2% respectively
Mixing Aspergillus and 4% saccharomycete are formulated, and the fermentation materials are prepared by agricultural byproducts, oil plant miscellaneous dregs of rice, keratin
Form；The mixing Aspergillus is by by 3:The black-koji mould and aspergillus oryzae of 1 ratio are mixed.
2. many effect yeast for animal feeds albumen powders according to claim 1, it is characterised in that：Three kinds of the black-koji mould can
Carbohydrase is produced respectively and produces the black-koji mould of pectase, mould xylan, cellulase and phytase generating by 1:1:1 ratio is mixed
Conjunction is formed.
3. many effect yeast for animal feeds albumen powders according to claim 1, it is characterised in that：The saccharomycete is Candida utilis
Saccharomycete.
4. many effect yeast for animal feeds albumen powders according to claim 1, it is characterised in that：The agricultural byproducts are maize
Any one in the bud dregs of rice, rice bran meal, pomace, jujube ground-slag, corn steep liquor.
5. many effect yeast for animal feeds albumen powders according to claim 1, it is characterised in that：The oil plant miscellaneous dregs of rice is cotton seed
Any one in the dregs of rice, the vegetable seeds dregs of rice, palm kernel meal, the ribes dregs of rice.
6. a kind of application of many effect yeast for animal feeds albumen powders in cultivation and feed manufacturing as claimed in claim 1.
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| CN201710677808.7A CN107223774A (en) | 2017-08-09 | 2017-08-09 | A kind of many effect yeast for animal feeds albumen powders and its application |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107836589A (en) * | 2017-12-08 | 2018-03-27 | 北京市希普正慧生物饲料有限公司 | Feed addictive of layer chicken and preparation method thereof and a kind of egg feedstuff |
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| CN101497910A (en) * | 2008-01-31 | 2009-08-05 | 海城市宏大牧业有限公司 | Method for preparing enzymolysis oligopeptide protein |
| CN104054907A (en) * | 2014-06-10 | 2014-09-24 | 湖南省农产品加工研究所 | Preparation method of protein feed |
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2017
- 2017-08-09 CN CN201710677808.7A patent/CN107223774A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101497910A (en) * | 2008-01-31 | 2009-08-05 | 海城市宏大牧业有限公司 | Method for preparing enzymolysis oligopeptide protein |
| CN104054907A (en) * | 2014-06-10 | 2014-09-24 | 湖南省农产品加工研究所 | Preparation method of protein feed |
Non-Patent Citations (2)
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| 宋善丹，等: "白酒糟固态发酵条件的筛选及营养价值评定", 《动物生产》 * |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107836589A (en) * | 2017-12-08 | 2018-03-27 | 北京市希普正慧生物饲料有限公司 | Feed addictive of layer chicken and preparation method thereof and a kind of egg feedstuff |
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Application publication date: 20171003 |