CN107173218A - A kind of method that mesh bag isolation external source pollen realizes plant selfing - Google Patents
A kind of method that mesh bag isolation external source pollen realizes plant selfing Download PDFInfo
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- CN107173218A CN107173218A CN201710558745.3A CN201710558745A CN107173218A CN 107173218 A CN107173218 A CN 107173218A CN 201710558745 A CN201710558745 A CN 201710558745A CN 107173218 A CN107173218 A CN 107173218A
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- Prior art keywords
- mesh bag
- plant
- bag
- mesh
- female fringe
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Botany (AREA)
- Developmental Biology & Embryology (AREA)
- Environmental Sciences (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention discloses the method that a kind of mesh bag isolation external source pollen realizes plant selfing, the mesh bag that mesh diameter is less than zasiokaurin diameter is enclosed on plant by this method before the female fringe spinning of plant, the sack of mesh bag is fixed on corn stem, fixed position is below female fringe, mesh bag is fixed on the brandreth above mesh bag by the rope at bag bottom, a certain distance is left between bag bottom and tassel.The mesh bag of the present invention is nylon material, translucency good permeability and can be reused, and simple in construction, easy to use, using effect is good；The completely isolated pollen of energy, external source pollution is not easily caused, significantly improves self-mating system breeding quality；Common artificial bagging method is needed to observe the spinning situation of plant female fringe, and mesh bag can be enclosed on plant by the present invention earlier before female fringe spinning, so this method is more time saving, it is laborsaving.
Description
Technical field
Plant selfing is realized the invention belongs to corn breeding field, more particularly to a kind of mesh bag isolation external source pollen
Method.
Background technology
Corn is the different flower of monoecism, and tassel life on the top of plant stalk, and female fringe life on the section in the middle part of stalk,
Cross-pollination rate is more than 95%.In order to which corn variety carries out pollination self, do not mixed by other type pollen, generally using people
Work bagging method isolates the pollen in other sources, to obtain purer selfed seed.Common artificial bagging method is：When entering
Enter florescence, the growth selection normal plant that physically well develops carries out bagging selfing.Will spin in the female fringe of elected plant and
When not spinning, female fringe is put with sodium sulphate paper bag；When female fringe spinning the one afternoon, then with larger sodium sulphate paper bag male
Inflorescence is put, and plays buffer action, it is to avoid different pollen contamination.Morning next day after male inflorescence bagging, after dew drying, with hero
Flower bag collects fresh pollen, invests rapidly on the female fringe filigree of homophyletic, and immediately the female fringe bagging isolation pollinated.In operation
During, if female fringe spinning period selection is improper, and the operation slow phenomenon for easily causing miscegenation during pollination.
Common artificial pollination is cumbersome, time-consuming；Paper bag hardly possible is fixed, easy to fall off during wind；If being rained after bagging,
Paper bag is easily drenched and airtight, so that can influence the quality of pollen influences pollination situation；And paper bag can not be reused,
Cause use cost high.
The content of the invention
It is an object of the invention to provide the method that a kind of mesh bag isolation external source pollen realizes plant selfing, it is intended to solves
Certainly common artificial pollination is cumbersome, time-consuming；Paper bag hardly possible is fixed, easy to fall off during wind；If being rained after bagging, paper bag is easy
Drenched and airtight, can influence the quality of pollen influences pollination situation；And paper bag can not be reused, use cost is high
The problem of.
The present invention is achieved in that before the female fringe spinning of plant is less than zasiokaurin diameter by mesh diameter
Mesh bag be enclosed on plant, the sack of mesh bag is fixed on corn stem, and fixed position leads to mesh bag below female fringe
The rope for crossing bag bottom is fixed on the brandreth above mesh bag, and a certain distance is left between bag bottom and tassel.
Further, described mesh bag is nylon material.
Further, the reticular density of described mesh bag is 300 mesh.
Effect collects
The mesh bag of the present invention is nylon material, translucency good permeability and can be reused, simple in construction, user
Just, using effect is good；The completely isolated pollen of energy, external source pollution is not easily caused, significantly improves self-mating system breeding quality；Common people
Work bagging method needs to observe the spinning situation of plant female fringe, and the present invention can be before female fringe spinning earlier by net
Bag is enclosed on plant, so this method is more time saving, it is laborsaving.
Brief description of the drawings
Fig. 1 is the use schematic diagram of mesh bag provided in an embodiment of the present invention；
In figure：1st, brandreth；2nd, mesh bag；3rd, plant.
Embodiment
In order to make the purpose , technical scheme and advantage of the present invention be clearer, it is right below in conjunction with drawings and Examples
The present invention is further elaborated.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention, and
It is not used in the restriction present invention.
Invention is achieved in that before the female fringe spinning of plant 3 is less than zasiokaurin diameter by mesh diameter
Mesh bag 2 be enclosed on plant 3, the sack of mesh bag 2 is fixed on corn stem, and fixed position is below female fringe, by net
Bag 2 is fixed on the brandreth 1 above mesh bag by the rope at bag bottom, and a certain distance is left between bag bottom and tassel.
Further, described mesh bag 2 is nylon material.
Further, the reticular density of described mesh bag 2 is 300 mesh.
Embodiment one, the effect to mesh bag isolation pollen are tested
Test material：There are 3 kinds for the corn variety of examination, totally 60 plants.When entering florescence, growth selection physically well develops normally
Plant tested.It is 120 mesh, 200 mesh, 300 mesh, 400 four kinds of mesh rule respectively from the nylon net bag of 4 kinds of different sizes
Lattice reticular density.
Test method：Experiment is in selfing experimental field middle progress.Female fringe is grown before filigree spues in plant, each
The female fringe of corn variety is respectively with respective 5 plants of bagging of 4 kinds of specification mesh bags, and sack is tightened with rope.After female fringe is spun, every plant of jade
Rice spread outside mesh bag above female fringe it is substantial amounts of with paper bag collection come pollen.After plant is solid, statistics is tied respectively
Bear fruit grains.
As a result with analysis：
The individual plant Cone crop statistical result that different size mesh bag is isolated is shown, the isolation effect of 400 mesh is best, individual plant
Average solid number is grain, and explanation can isolate external source pollen.300 mesh, the average solid number of individual plant is grain, 200 purposes.Through overtesting
Compare, reticular density brought up into 300 mesh, almost can completely isolated external source pollen, significantly improve self-mating system breeding quality.
Embodiment two, the effect to mesh bag selfing are tested
Test material：There are three kinds for the corn variety of examination.Totally 90 plants.When entering florescence, growth selection physically well develops just
Normal plant is tested.The pollen isolation mesh bag 45 and sodium sulphate paper bag 90 of 300 mesh.
Test method：Experiment is in selfing experimental field middle progress.15 plants of each kind of plant of three kinds, is divided to two groups
Tested, one group carries out selfing using this method, i.e., using mesh bag, before maize ear spinning, mesh bag is placed on corn
On plant.When tassel starts loose powder, and female fringe also begins to spinning, plant is periodically rocked.Another group using commonly artificial
Bagging method carries out selfing, i.e. paper bag, will be female with sodium sulphate paper bag when will spin and not spin in the female fringe of the plant when choosing
Fringe is put；When female fringe spinning the puts male inflorescence one afternoon, then with larger sodium sulphate paper bag, buffer action is played, it is to avoid
Different pollen contamination.In morning next day after male inflorescence bagging, after dew drying, fresh pollen is collected with male flower bag, is awarded rapidly
In on the female fringe filigree of homophyletic, and immediately the female fringe bagging isolation pollinated.After plant is solid, fruit is taken on the same day
Fringe, counts Cone crop respectively.
As a result with analysis：
Significance test is carried out using SPSS softwares to data above, judges that the difference of two methods individual plant Cone crop shows
Work property.
F examines (LSD methods)：
Analysis of variance table：
Significance test result shows, in 3 corn varieties, and the solid situation of individual plant of mesh bag is notable with common paper bag
Sex differernce is little.
The mesh bag of the present invention is nylon material, translucency good permeability and can be reused, simple in construction, user
Just, using effect is good；The completely isolated pollen of energy, external source pollution is not easily caused, significantly improves self-mating system breeding quality；Common people
Work bagging method needs to observe the spinning situation of plant female fringe, and the present invention can be before female fringe spinning earlier by net
Bag is enclosed on plant, so this method is more time saving, it is laborsaving.
Although above-mentioned the embodiment of the present invention is described with reference to accompanying drawing, not to present invention protection model
The limitation enclosed, one of ordinary skill in the art should be understood that on the basis of technical scheme those skilled in the art are not
Need to pay various modifications or deform still within protection scope of the present invention that performing creative labour can make.
Claims (3)
1. a kind of method that mesh bag isolation external source pollen realizes plant selfing, it is characterised in that described mesh bag isolation is outer
It is straight that source pollen realizes that mesh diameter is less than zasiokaurin by the method for plant selfing before the female fringe spinning of plant
The mesh bag in footpath is enclosed on plant, and the sack of mesh bag is fixed on corn stem, and fixed position is below female fringe, by mesh bag
It is fixed on by the rope at bag bottom on the brandreth above mesh bag, a certain distance is left between bag bottom and tassel.
2. the method that mesh bag isolation external source pollen as claimed in claim 1 realizes plant selfing, it is characterised in that described
Mesh bag be nylon material.
3. the method that mesh bag isolation external source pollen as claimed in claim 1 realizes plant selfing, it is characterised in that described
Mesh bag reticular density be 300 mesh.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710558745.3A CN107173218A (en) | 2017-07-11 | 2017-07-11 | A kind of method that mesh bag isolation external source pollen realizes plant selfing |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710558745.3A CN107173218A (en) | 2017-07-11 | 2017-07-11 | A kind of method that mesh bag isolation external source pollen realizes plant selfing |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107173218A true CN107173218A (en) | 2017-09-19 |
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ID=59844840
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710558745.3A Pending CN107173218A (en) | 2017-07-11 | 2017-07-11 | A kind of method that mesh bag isolation external source pollen realizes plant selfing |
Country Status (1)
| Country | Link |
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| CN (1) | CN107173218A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107211680A (en) * | 2017-05-27 | 2017-09-29 | 安徽信福乡田生态农业有限公司 | A kind of implantation methods of corn |
| CN111758549A (en) * | 2019-12-07 | 2020-10-13 | 山西省农业科学院小麦研究所 | Isolation and pollination method for single sunflower plant |
-
2017
- 2017-07-11 CN CN201710558745.3A patent/CN107173218A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107211680A (en) * | 2017-05-27 | 2017-09-29 | 安徽信福乡田生态农业有限公司 | A kind of implantation methods of corn |
| CN111758549A (en) * | 2019-12-07 | 2020-10-13 | 山西省农业科学院小麦研究所 | Isolation and pollination method for single sunflower plant |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20170919 |
|
| WD01 | Invention patent application deemed withdrawn after publication |