CN107018890A - Application of the nylon wire as isolated material on corn and corn Wild related germplasm breeding - Google Patents
Application of the nylon wire as isolated material on corn and corn Wild related germplasm breeding Download PDFInfo
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- CN107018890A CN107018890A CN201710231477.4A CN201710231477A CN107018890A CN 107018890 A CN107018890 A CN 107018890A CN 201710231477 A CN201710231477 A CN 201710231477A CN 107018890 A CN107018890 A CN 107018890A
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- nylon
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- net bag
- solarium
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
Abstract
Application the invention discloses nylon wire as isolated material on corn and corn Wild related germplasm breeding；The aperture of the nylon wire is 38~53 μm；The invention also discloses the nylon net bag selfing instrument for corn Wild related germplasm selfing.In addition, also disclosing the nylon solarium available for self-mating system breeding, cross combination preparation and transgenic line experiment.Nylon net bag or nylon solarium isolation effect of the present invention is good, can completely isolated foreign pollen；Secondly, its translucency and good permeability do not influence on growing for corn；In addition, the method setting percentage for carrying out corn Wild related germplasm selfing using the present invention is significantly improved, and it is more time saving than conventional method, it is laborsaving, substantially reduce cost of labor；In addition, nylon solarium of the present invention is not limited by time, place, it can be arranged as required to whenever and wherever possible；Nylon net bag or nylon solarium of the present invention is reusable, economizes on resources, reduces environmental pollution, the cost of material is low.
Description
Technical field
The invention belongs to corn breeding techniques field, and in particular to nylon wire is wild in corn and the nearly edge of corn as isolated material
Purposes in non-hibernating eggs breeding；A kind of nylon net bag and a kind of nylon solarium are further related to, and utilizes nylon net bag or nylon solarium
The method for carrying out corn or the selfing of corn Wild related germplasm or hybridization.
Background technology
Corn (Zea mays L.) is earliest using one of crop of hybrid vigour, and self-mating system, then profit are cultivated by selfing
The basic skills that cenospecies is corn breeding is combined with different self-mating systems.Therefore, selfing is the important technology hand of corn breeding
Section.Corn is typical monoecism cross-pollinatd plant, and tassel is born in plant top, and female fringe is born in stipes in the middle part of stalk
On, its cross-pollination rate is more than 95%.In order to avoid mixing the pollen in other sources, generally using artificial set during corn selfing
Bag method isolates the pollen in other sources, to obtain selfed seed.Common artificial bagging method is：In the female of elected plant
When fringe will spin and not spin, female fringe is put with sodium sulphate paper bag；In the previous day of pollination, with larger sodium sulphate paper bag
Or kraft paper bag puts the tassel of same plant, its purpose has been mainly buffer action, it is to avoid other plant pollens pollute；
In morning next day after tassel bagging, fresh pollen is collected with the paper bag being enclosed on tassel, is invested rapidly on the female fringe filigree of homophyletic,
And immediately by the female fringe bagging isolation pollinated.The subject matter that above-mentioned artificial bagging method is present：(1) if pollinated to female fringe
It is improper that period is chosen, and setting percentage can be caused low；(2) if operating the pollen lack of standardization that easily causes to mix pollination now when pollinating
As；(3) it is cumbersome, it is time-consuming；(4) paper bag hardly possible is fixed, easy to fall off during wind；(5) if rain, paper bag easily drenched and
And it is airtight, the quality of pollen can be influenceed, and then influence Pollination Effect；(6) paper bag is typically only capable to use 2~3 times, it is impossible to repeatedly
Recycling, the problem of there is waste and high cost.The selfing problem of corn Wild related germplasm is more prominent.
Compared with corn, all there is larger difference in corn Wild related germplasm on plant forms and fringe portion form.Such as corn
Wild related germplasm plant is widely present tiller phenomenon, and tiller number is sometimes more than 40, and single plant has multiple tassels；Separately
Outside, the female fringe more piece position of corn Wild related germplasm it is raw, female fringe is small and multiple fringe fasciations together, single tassel seed number 6~12,
It is grown in inside axil, the fringe of fasciation does not stretch out also when usually spinning.Therefore, during the selfing of corn Wild related germplasm, exist not
Not the problems such as easy bagging, bagging shakiness and bagging are not easy to string powder sternly, and the selfed seed obtained is less.
In corn breeding, it is also often desirable to propagating corn self-mating system or prepare corn hybrid seed on a small quantity, due to corn
Cross-pollination characteristic, generally isolated using space or the method for long-stalked crops isolation set isolated area, this method, which exists, looks for
Seek that suitable isolated area is relatively difficult, land wastage is larger, and isolation is the problems such as be difficult to thorough, be also asking for urgent need to resolve
Topic.
Strict isolation condition is needed in transgenic corns experiment, but is due to the characteristic of corn cross-pollination, Yi Jiyu
The pollen amount of rice is big, easily racks and is scattered to larger distance, thus is easier to cause the pollution of alien gene.
Nylon (Nylon) net be using nylon yarn, Polyamide Yarns, dacron thread, receive synthetic fibre silk etc. chemical fibre as raw material, through wire mesh machine
The chemical fibre mesh grid that tool is processed, nylon wire is the general name of these chemical fibre mesh grids.Nylon wire has that toughness is high, elasticity is good,
The features such as corrosion-resistant, oil resistant, water-fast, wear-resisting, high temperature resistant, weatherability；Also there is good insulating, the low characteristic of lubricating coefficient.Buddhist nun
Imperial net is widely used in industrial filter, oil, chemical industry, printing, fishery and the industry such as fishes for.Nylon wire is agriculturally being mainly used in plant
Thing sunshade, seed packaging, seed soaking, insect prevention, anti-drosophila, anti-bird and poultry farming etc..Patent《Nicotianagossei seedling net chamber》
(Application No.：2010201695518) a kind of nylon solarium and a kind of sowing mesh grid are disclosed, but the net of its nylon solarium
Eye density is 40~60 mesh, and its aperture is far longer than tobacco pollen grain diameter and popcorn powder diameter, and it is mainly used in insect prevention,
Buffer action to pollen is poor.
Through retrieval, the report that nylon wire is applied as isolated material on corn and corn Wild related germplasm breeding is not found
Road.
The content of the invention
For complex operation, setting percentage are low present on current corn and corn Related species artificial pollination, easily mix, into
The problems such as this is high, present invention aims at provide nylon wire as isolated material on corn or corn Wild related germplasm breeding
Using.
Another object of the present invention is to provide a kind of nylon net bag.
3rd purpose of the invention is to provide the application that above-mentioned nylon net bag is handed in certainly in corn or corn Wild related germplasm.
4th purpose of the invention, which is to provide, utilizes above-mentioned nylon net bag progress corn or corn Wild related germplasm selfing
Method.
5th purpose of the invention is to provide a kind of nylon solarium.
6th purpose of the invention is to provide application of the above-mentioned nylon solarium as isolated area on corn breeding.
7th purpose of the invention is to provide purposes of the above-mentioned nylon solarium in corn inbred line breeding.
8th purpose of the invention is to provide the method using above-mentioned nylon solarium propagating corn self-mating system.
9th purpose of the invention is to provide purposes of the above-mentioned nylon solarium on Maize hybridized combination is prepared.
Tenth purpose of the invention is to provide the method for preparing corn hybrid seed using above-mentioned nylon solarium.
11st purpose of the invention is to provide application of the above-mentioned nylon solarium in the transgenic corns testing of materials.
12nd purpose of the invention is to provide the method for carrying out transgenic corns experiment using above-mentioned nylon solarium.
To achieve the above object, technical scheme is as follows：
The invention provides nylon wire as isolated material in corn (Zea mays L.) or corn Wild related germplasm breeding
On application.
Described nylon wire is formed by nylon (or polyamide fibre, English is Nylon) monofilament or mariages braiding, nylon
Entitled Silon (polyamide fibre).
The aperture of described nylon wire is 38~53 μm；Or reticular density is 300~420 mesh.
Described corn Wild related germplasm refers to Zea diploperennis (Zea diploperennis), class in great numbers
Maize seed (Zea luxurians), annual teosinte (Zea mexicana), small clever class corn (Zea parviglumis),
Nicaragua's class corn (Zea nicaraguensis), tetraperennial teosinte (Zea perennis), committee of committee Te Nange
Class corn (Zea huehuetenangensis) or friction standing grain (Tripsacumdactyloides) etc..
Present invention also offers a kind of nylon net bag, the length of described nylon net bag is 1.0~1.8m, and width is 0.5
~1.2m；Wherein the mesh aperture of nylon wire is 38~53 μm；Or reticular density is 300~420 mesh.
The diameter of corn (Zea mays L.) pollen grain is about 94.15~118.83 μm, average 106.14 ± 4.68 μm；
The diameter of corn Wild related germplasm pollen grain is about 65.00~95.00 μm, average 85.11 ± 3.04 μm.Described nylon wire
Aperture is less than the diameter of corn or corn Wild related germplasm pollen grain.
The size of above-mentioned nylon net bag is can be the female fringe above section of plant or corn Wild related germplasm plant
It is inserted in nylon net bag.
Around above-mentioned nylon net bag, its bag in addition to sack all be suture.
Present invention also offers above-mentioned nylon net bag on corn (Zea mays L.) or corn Wild related germplasm breeding
Using.
Described corn Wild related germplasm refers to Zea diploperennis (Zea diploperennis), class in great numbers
Maize seed (Zea luxurians), annual teosinte (Zea mexicana), small clever class corn (Zea parviglumis),
Nicaragua's class corn (Zea nicaraguensis), tetraperennial teosinte (Zea perennis), committee of committee Te Nange
Class corn (Zea huehuetenangensis) or friction standing grain (Tripsacum dactyloides) etc..
Present invention also offers a kind of nylon net bag selfing instrument, including above-mentioned nylon net bag 1, in the bag of nylon net bag 1
Mouth is sewed with rope 2, and in the bottom of nylon net bag 1, two jiaos are fixed with rope 3, and the bottom of nylon net bag 1 is fixed on by rope 3
On crossbeam 4, the two ends of crossbeam 4 are individually fixed on column 5A and column 5B by fastener 6A and 6B, and column 5A and column 5B are solid
Due on the ground；Its middle cross beam 4 is 2~3 meters away from ground vertical height.
The length of nylon net bag described in above-mentioned nylon net bag selfing instrument be 1.0~1.8m, width be 0.5~
1.2m；The aperture of its nylon wire is 38~53 μm, or reticular density is 300~420 mesh.
The material of crossbeam or column described in above-mentioned nylon net bag selfing instrument is steel pipe or iron pipe etc..
By the first purpose that the bottom of nylon net bag is fixed on crossbeam it is not allow Buddhist nun in above-mentioned nylon net bag selfing instrument
Imperial mesh bag bottom is pressed onto tassel, in order to avoid the development of influence tassel；Another object is that playing fixed corn or corn Wild related germplasm
The effect of plant, it is to avoid the lodging of corn or corn Wild related germplasm plant.
The application that above-mentioned nylon net bag selfing instrument is handed in certainly in corn or corn Wild related germplasm.
Present invention also offers the method that corn or corn Wild related germplasm plant selfing are carried out using above-mentioned nylon net bag,
Before the female fringe spinning for being included in corn or corn Wild related germplasm, by the bag of the nylon net bag in above-mentioned nylon net bag selfing instrument
Mouth is enclosed on corn or corn Wild related germplasm plant from the top down, and the sack of nylon net bag is fixed on corn or jade by rope
In the stalk of rice Wild related germplasm plant, the position that sack is fixed is located at below female fringe；Nylon net bag is patted in the loose powder phase or is shaken
Shake plant.
Present invention also offers a kind of nylon solarium, described nylon solarium includes frame structure, is covered outside frame structure
There is the nylon wire that pore size is 38~53 μm (or reticular density is 300~420 mesh)；Described frame structure is by steel pipe and company
Fitting is constituted, and described steel pipe includes 4 steel pipe posts, and 4 steel pipe posts are carried out respectively by connector with 4 steel pipe crossbeams
Connection, 4 steel pipe posts are fixed on the ground；Described nylon wire is made up of top nylon wire and surrounding nylon wire；In surrounding Buddhist nun
One side of dragon net sets a side door, and the nylon wire of side door both sides is using corresponding button-shaped connection, or zip mode connection；
The height of described nylon solarium is 1.8~3.0 meters.
The height of above-mentioned nylon solarium should be higher than that the height of plant and the height of people, it is therefore an objective to make corn or corn near
Growing and pollinate etc. for edge wild species is unaffected, and is easy to artificial operation.
The bottom position of surrounding nylon wire described in above-mentioned nylon solarium can be contiguously face, or for ground with
On, and less than the position of 20~30cm below lowest order female fringe.
The length and width of described nylon solarium is according to isolated area size according to being actually needed setting.
The purpose of side door is set to be to facilitate breeder to pass in and out in above-mentioned nylon solarium, while to ensure that closure is good, with
Exempt from foreign pollen entrance.
Present invention also offers above-mentioned nylon solarium as isolated area corn (Zea mays L.) self-mating system breeding on
Using.
Present invention also offers the method using above-mentioned nylon solarium propagating corn self-mating system, including by corn inbred line kind
Plant in above-mentioned nylon solarium；Or before corn inbred line seedling stage or female fringe spinning, set in corn inbred line planting area
Above-mentioned nylon solarium；In the free pollination of loose powder phase.
Present invention also offers application of the above-mentioned nylon solarium on Maize hybridized combination is prepared.
Present invention also offers the method that Maize hybridized combination is prepared using above-mentioned nylon solarium, it is included in female many fathers'
In the case of, maternal self-mating system is planted in above-mentioned nylon wire room, or before maternal self-mating system seedling stage or spinning it is maternal from
Friendship is that planting area sets above-mentioned nylon solarium；By maternal self-mating system artificial emasculation before loose powder, and different male parents are gathered respectively
The pollen of self-mating system gives maternal self-mating system pollination, and marks；Fruit ear is harvested when ripe respectively.
Present invention also offers the method that Maize hybridized combination is prepared using above-mentioned nylon solarium, it is included in many mothers' of a father
In the case of, different maternal self-mating systems are sequentially planted in above-mentioned nylon wire room, and at interval of the maternal self-mating system kind of 4~6 rows
Plant 1 row male parent self-mating system；Or before seedling stage or the spinning of earliest maternal self-mating system, set on the planting area of maternal self-mating system
Put above-mentioned nylon solarium；By maternal self-mating system artificial emasculation before loose powder, male parent self-mating system free pollination is harvested maternal when ripe
Fruit ear in self-mating system, and mark.
, will in above-mentioned nylon wire room present invention also offers the method using the above-mentioned nylon solarium propagating corn seed of single cross
Father, maternal self-mating system are 1 according to parental:4~6 proportional spacing plantation；Or in seedling stage or maternal self-mating system spinning
Before, at interval, father and mother's self-mating system planting area of plantation sets above-mentioned nylon solarium；Maternal self-mating system is manually gone before loose powder
Hero, male parent self-mating system free pollination harvests the fruit ear on maternal plant, produces Corn Single-Cross Stock seed when ripe.
Present invention also offers application of the above-mentioned nylon solarium in the transgenic corns testing of materials.
It is present invention also offers the method that the transgenic corns testing of materials is carried out using above-mentioned nylon solarium, transgenosis is beautiful
Rice material is planted in above-mentioned nylon solarium, carries out artificial or spontaneous pollination in the loose powder phase, fruit ear is harvested when ripe.
Compared with prior art, the present invention has the advantage that and beneficial effect：(1), nylon net bag or nylon wire of the present invention
The material of room is nylon wire, translucency and good permeability, and growing for corn is not influenceed；In addition, the size energy of mesh bag
It is enough that the female fringe above section of plant is inserted in mesh bag, do not damage maize leaf.(2), the nylon wire used in the present invention
The completely isolated external source zasiokaurin of energy, does not result in external source pollen contamination.(3), nylon net bag or nylon wire cell structure of the present invention
Simply, easy to use, isolation effect is good.(4), the present invention carries out the method ratio of corn Wild related germplasm selfing using nylon net bag
Traditional disposable artificial pollination method setting percentage is significantly improved, and is rocked mainly due to the external world or the reason for wind, flower
Powder, which can persistently fall down, gives the pollination of female fringe filigree, therefore its setting percentage is significantly improved.(5), the present invention utilizes nylon net bag
Carry out selfing method can before female fringe spinning earlier when nylon net bag is enclosed on corn or the nearly edge of corn is wild
On plant, it is not necessary to observe the spinning of plant female fringe or tassel loose powder situation as traditional artificial bagging pollination, save
When, it is laborsaving, greatly reduce cost of labor.(6), corn Wild related germplasm due to female fringe it is many and small, developed not between each female fringe
Unanimously, common artificial bagging method workload is big, setting percentage is low, using nylon net bag of the present invention is convenient and strength-saving and setting percentage
It is high.(7), nylon net bag of the present invention can be reused, and save resource, decreased the pollution to environment, reduced material
Cost.(8), nylon solarium of the present invention is not limited by time, place, can be arranged as required to whenever and wherever possible, and can be repeated
Use, can also factorial praluction, breeding efficiency significantly improves.(9), nylon solarium of the present invention provides for transgenic line experiment
Good isolating tool, it is simple and efficient, and do not limited by time, place.
Brief description of the drawings
Fig. 1 is nylon net bag schematic diagram of the present invention.
Fig. 2 is nylon net bag selfing instrument schematic diagram of the present invention；Wherein 1 is nylon net bag, and 2 be rope, and 3 be rope, and 4 are
Crossbeam, 5A and 5B are column, and 6A and 6B are fastener.
Fig. 3 is nylon solarium of the present invention schematic diagram；Wherein A is steel pipe post, and B is steel pipe crossbeam, and C is top nylon wire, D
For surrounding nylon wire, E is side door, and F is connector.
Embodiment
The nylon wire isolation pollen effect test of the different reticular densities of embodiment 1
(1), test material：
1st, it is RP125,18-599 and SAU 08-641 respectively, all by Sichuan Agricultural University for examination corn inbred line totally 3
Corn research institute seed selection, seed is provided by corn research institute of Sichuan Agricultural University.
2nd, the specification of nylon wire (nylon wire is purchased from Taizhou Xiong Ding screen clothes Co., Ltd)：Reticular density is respectively 120 mesh (holes
Footpath：125 μm), 200 mesh (apertures：75 μm), 300 mesh (apertures：48 μm) or 400 mesh (apertures：37μm).
(2) making of nylon net bag
The reticular density of this experiment nylon wire used is respectively 120 mesh, 200 mesh, 300 mesh and 400 mesh.In order to avoid flower
Powder enters, and using sewing machine by nylon wire two-wire bound edge sewing into open-topped nylon net bag, the width of nylon net bag is
0.15 meter, length is 0.4 meter.
(3) test method：
This experiment is carried out in the refined Anduo County's battalion's corn breeding base of Sichuan Agricultural University.Each self-mating system plants 4 rows, often row 14
Strain.Before the spinning of plant female fringe, the good normal plant that grows is randomly selected, be respectively 120 mesh with reticular density,
200 mesh, 300 mesh or 400 mesh totally 4 kinds of specifications, 5 female fringes of the nylon net bag of every kind of specification respectively to each self-mating system are carried out
Bagging, sack is tightened with rope；After after female fringe spinning, external source zasiokaurin is collected with paper bag, then in the Buddhist nun being enclosed in female fringe
Zasiokaurin is largely spread top position outside imperial mesh bag to filigree position；Fruit ear is harvested after 40 days after corn pollination, is united respectively
Count Cone crop.
The nylon wire isolation zasiokaurin effect test result of the different reticular densities of table 1
As a result (1 is shown in Table) when the reticular density of nylon wire is 120 mesh, and it is 13.2~15.6 that individual plant is averagely solid, every
It is worst from effect；When nylon net bag reticular density is 200 mesh, it is 2.8~4.0 that individual plant is averagely solid, and isolation effect is poor；
When nylon net bag reticular density is 300 mesh and 400 mesh, the solid kernal number of individual plant is 0, and isolation effect preferably, can be complete
Isolate external source zasiokaurin.When the above results illustrate reticular density when nylon wire higher than 300 mesh (aperture is less than 53 μm), nylon
The completely isolated external source zasiokaurin of net energy, can be applied as isolated material in corn breeding.
Embodiment 2 carries out the Piglet s colibacillosis of corn selfing using nylon net bag of the present invention.
(1) test material：
(1), for examination corn inbred line 3, respectively RP125,18-599 and SAU08-641,3 self-mating systems are by Sichuan agriculture
Sparetime university learns corn research institute and provided.
(2), specification is the nylon net bag 45 of 300 mesh, sodium sulphate paper bag 90.
(2) making of nylon net bag and nylon net bag selfing instrument
(1) making of nylon net bag：The nylon wire that reticular density is 300 mesh is taken, is 1.2 according to the width of nylon net bag
Rice, length is 1.8 meters and cut, and justified margin is folded twice, then using sewing machine with 0.5~1.0 centimetre of broadband
Sewing, and be sack by one end sewing, produce nylon net bag of the present invention (see Fig. 1).
(2) making of nylon net bag selfing instrument of the present invention：First column 5A and column 5B are fixed on the ground, so-called fixation
Fixed in insertedly or by cement base；One end of crossbeam 4 is fixed on column 5A by fastener 6A, the other end passes through
Fastener 6B is fixed on column 5B, and vertical height of the crossbeam 4 apart from ground is 2~3 meters, and two jiaos of the bottom of nylon net bag 1 is tied up
Upper or sewing rope 3, the two ends of rope 3 are tied up on crossbeam 4, tied rope 2 are sewed on the sack of nylon net bag 1, Buddhist nun of the present invention is produced
Imperial mesh bag selfing instrument (see Fig. 2).Above-mentioned column 5A, column 5B are steel pipe for building, and 6A and 6B are fastener for building.
(3) test method：
(1) this experiment is carried out in the refined Anduo County's battalion's corn breeding base of Sichuan Agricultural University.Experimental design is RP125,18-
599 and SAU08-641 totally 3 corn inbred lines, plant 2 rows, often 14 plants of row, are divided to two groups to be tested respectively, and one group using this
Invention nylon net bag selfing instrument (see Fig. 2) carries out selfing, before maize ear spinning, by the sack of nylon net bag 1 from upper
It is placed on plant downwards, sack is located at below female fringe, and is fixed on rope 2 on plant；When tassel starts loose powder,
When female fringe also begins to spinning, plant is periodically rocked.Another group carries out selfing using common artificial bagging method, in experiment
When the female fringe of plant will spin and not spin, female fringe is put with sodium sulphate paper bag；After female fringe is spun, and tassel starts to dissipate
After powder, before the afternoon before that day dew of pollination is dried, male flower is put using larger sodium sulphate paper bag, clip is utilized
It is fixed.In morning next day after tassel bagging, after dew drying, fresh pollen is collected with the paper bag being enclosed on tassel, rapidly
Invest on the female fringe filigree of homophyletic, and the female fringe bagging pollinated is prevented string powder immediately.40 days after plant pollinates, receive
Fruit ear is obtained, solid kernal number is counted respectively.
Table 2 utilizes the different solid comparative test results of autocopulation method corn inbred line
As a result (2 are shown in Table) and are all shown using the average solid kernal number obtained by nylon net bag selfing instrument of the present invention progress selfing
Write higher than the average solid kernal number obtained by traditional artificial bagging pollinating method.Illustrate to utilize nylon net bag ratio using the present invention
The Pollination Effect of paper bag is good, can significantly improve the setting percentage of corn；And it is simple to operate, time saving and energy saving, cost is low.
Embodiment 3 carries out corn Wild related germplasm selfing Piglet s colibacillosis using nylon net bag
(1) test material：
(1) it is, annual teosinte subspecies (Zea mexicana) 3, respectively Z1, Z2 for examination corn Wild related germplasm
And Z3, the material is by the offer of corn research institute of Sichuan Agricultural University.
(2), specification is the nylon net bag 15 of 300 mesh, sodium sulphate paper bag 60.
(2) making (be the same as Example 2) of nylon net bag selfing instrument.
(3) test method (be the same as Example 2).
The solid comparative test result of the corn Wild related germplasm of table 3 difference autocopulation method
As a result (3 are shown in Table) and selfing is carried out to annual teosinte using nylon net bag selfing instrument of the present invention, its individual plant is put down
Solid kernal number is all significantly higher than traditional artificial bagging pollinating method.Illustrate to hand over certainly using nylon net bag using the present invention
Tool is better than traditional paper bag Pollination Effect, can significantly improve the self-fruitful rate of corn Wild related germplasm；And it is simple to operate,
Time saving and energy saving, cost is low.
Embodiment 4 carries out the contrast test of corn selfing using nylon solarium of the present invention
(1), test material：
1st, it is RP125,18-599 and SAU08-641 respectively, by Sichuan Agricultural University's jade for examination corn inbred line totally 3
Rice research institute seed selection, seed is provided by corn research institute of Sichuan Agricultural University.
(2) nylon solarium is built
Nylon solarium (see Fig. 3) is built using materials such as steel pipes, using steel pipe as framework, first by 4 steel pipe post A (4
Column can numbering A1, A2, A3 and A4 respectively) the insertion cm of underground 40；Then using 4 steel pipe crossbeam B, (4 crossbeams can
Numbering is B1, B2, B3 and B4 respectively), top is connected respectively, infall is fixed using steel tube fastener F.Caping is cladded with framework
Portion nylon wire C and surrounding nylon wire D (C and D can be sewed together into nylon guard), is set in a side of surrounding nylon wire
One side door E, is left a door unlocked turnover using 1.5 meters of slide fasteners, in order to effectively prevent pollen from entering, and nylon net cloth extends 30 centimetres and blocks drawing
Chain.The reticular density specification for the nylon wire being wherein covered in outside steelframe is respectively 120 mesh, 200 mesh, 300 mesh or 400 mesh.This
The height for testing nylon solarium is 2.5 meters, and width is 3.5 meters, and length is 5.0 meters.Respectively according to framework length chi
It is very little, increase by 10 centimetres, the nylon net cloth of different reticular densities is sewn into nylon guard.
(3) test method：
Field test is carried out in the refined Anduo County's battalion's corn breeding base of Sichuan Agricultural University.Three railway carriage or compartments of parallel plantation, it is each per railway carriage or compartment
Self-mating system plants 2 rows, and often 14 plants of row, are repeated 4 times.When entering florescence, the middle railway carriage or compartment plant of selection builds reticular density and is respectively
The nylon solarium of 120 mesh, 200 mesh, 300 mesh or 400 mesh, totally 4 kinds of different sizes.Completed before female fringe is grown, using band leaf
Emasculation method removes out maize male ears.After the fruit ear after corn pollination 40 days in harvest nylon wire room, 10 fruit ears are randomly selected, respectively
Count Cone crop.
The different size nylon net isolation method corn external source pollen effect test result of table 4
As a result (4 are shown in Table) when nylon solarium reticular density is 120 mesh, it is 11.7~14.6 that individual plant is averagely solid, every
It is worst from effect；When nylon solarium reticular density is 200 mesh, it is 3.7~4.6 that individual plant is averagely solid, and isolation effect is poor；Buddhist nun
When imperial solarium's reticular density is 300 mesh and 400 mesh, the average solid seed of individual plant is 0, isolation effect preferably, can completely every
From external source zasiokaurin.The above results illustrate that nylon solarium reticular density, can be completely isolated higher than 300 mesh (aperture is less than 53 μm)
External source zasiokaurin, can as isolated area corn inbred line breeding, cross combination is assembled, corn hybrid seed is bred, turn base
Because being applied in the corn breedings such as the testing of materials.
Claims (13)
1. application of the nylon wire as isolated material on corn (Zea mays L.) or corn Wild related germplasm breeding；Wherein
The aperture of the nylon wire is 38~53 μm；Or reticular density is 300~420 mesh.
2. application according to claim 1, it is characterised in that described corn Wild related germplasm refers to Diploid Perennial
Class corn (Zea diploperennis), class corn in great numbers (Zea luxurians), annual teosinte (Zea
Mexicana), small clever class corn (Zea parviglumis), Nicaragua's class corn (Zea nicaraguensis), four times
The perennial class corn of body (Zea perennis), committee's committee's Te Nange classes corn (Zea huehuetenangensis) or friction standing grain
(Tripsacum dactyloides)。
3. a kind of nylon net bag, it is characterised in that the length of described nylon net bag is 1.0~1.8m, width is 0.5~1.2m；
Wherein the mesh aperture of nylon wire is 38~53 μm.
4. application of the nylon net bag on corn (Zea mays L.) or corn Wild related germplasm breeding described in claim 3；
Wherein described corn Wild related germplasm refers to Zea diploperennis (Zea diploperennis), class corn in great numbers
Kind (Zea luxurians), annual teosinte (Zea mexicana), small clever class corn (Zea parviglumis), Buddhist nun add
Draw melon corn (Zea nicaraguensis), tetraperennial teosinte (Zea perennis), committee of committee Te Nange classes beautiful
Rice (Zea huehuetenangensis) or friction standing grain (Tripsacum dactyloides).
5. a kind of nylon net bag selfing instrument, it is characterised in that including the nylon net bag (1) described in claim 3, in nylon wire
The sack of bag (1) is sewed with rope (2), and in the bottom of nylon net bag (1), two jiaos are fixed with rope (3), the bottom of nylon net bag (1)
Portion is fixed on crossbeam (4) by rope (3), and the two ends of crossbeam (4) are individually fixed in vertical by fastener (6A) and fastener (6B)
On post (5A) and column (5B), column (5A) and column (5B) are fixed on the ground；Its middle cross beam (4) is 2 away from ground vertical height
~3 meters；The length of described nylon net bag (1) is 1.0~1.8m, and width is 0.5~1.2m；The aperture of its nylon wire be 38~
53 μm, or reticular density is 300~420 mesh.
6. the application that the nylon net bag selfing instrument described in claim 5 is handed in certainly in corn or corn Wild related germplasm.
7. the side of corn or corn Wild related germplasm plant selfing is carried out using the nylon net bag selfing instrument described in claim 5
Method, it is characterised in that before the female fringe spinning for being included in corn or corn Wild related germplasm, by the nylon net bag described in claim 5
The sack of nylon net bag in selfing instrument is enclosed on corn or corn Wild related germplasm plant from the top down, the bag of nylon net bag
Mouth is fixed on by rope in the stalk of corn or corn Wild related germplasm plant, and the position that sack is fixed is located at below female fringe；
Nylon net bag is patted in the loose powder phase or rocks plant.
8. a kind of nylon solarium, it is characterised in that described nylon solarium includes frame structure, and aperture is covered with outside frame structure
Size is the nylon wire of 38~53 μm (or reticular density is 300~420 mesh)；Described frame structure is by steel pipe and connector group
Into, described steel pipe includes 4 steel pipe posts, and 4 steel pipe posts are attached respectively by connector with 4 steel pipe crossbeams, and 4
Individual steel pipe post is fixed on the ground；Described nylon wire is made up of top nylon wire and surrounding nylon wire；In surrounding nylon wire
One side sets a side door, and the nylon wire of side door both sides is using corresponding button-shaped connection, or zip mode connection；Described
The height of nylon solarium is 1.8~3.0 meters；The bottom position of described surrounding nylon wire for contact ground, or for ground with
On, and less than the position of 20~30cm below lowest order female fringe.
9. application of the nylon solarium as isolated area in the breeding of corn (Zea mays L.) self-mating system described in claim 8.
10. application of the nylon solarium on Maize hybridized combination is prepared described in claim 8.
11. the method for Maize hybridized combination is prepared using the nylon solarium described in claim 8, it is characterised in that in female many fathers
In the case of, maternal self-mating system is planted in the nylon wire room described in claim 8, or in maternal self-mating system seedling stage or is told
The nylon solarium described in claim 8 is set in maternal self-mating system planting area before silk；It is before loose powder that maternal self-mating system is artificial
Emasculation, and the pollen of different male parent self-mating systems is gathered respectively to maternal self-mating system pollination, and mark；Fruit is harvested when ripe respectively
Fringe.
12. the method for Maize hybridized combination is prepared using the nylon solarium described in claim 8, it is characterised in that more female in a father
In the case of, different maternal self-mating systems are sequentially planted in the nylon wire room described in claim 8, and at interval of 4~6 rows
Maternal self-mating system plants 1 row male parent self-mating system；Or before seedling stage or the spinning of earliest maternal self-mating system, in maternal self-mating system
Nylon solarium described in claim 8 is set on planting area；By maternal self-mating system artificial emasculation, male parent self-mating system before loose powder
Free pollination, harvests the fruit ear in maternal self-mating system, and mark when ripe.
13. application of the nylon solarium in the transgenic corns testing of materials described in claim 8.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710231477.4A CN107018890A (en) | 2017-04-11 | 2017-04-11 | Application of the nylon wire as isolated material on corn and corn Wild related germplasm breeding |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710231477.4A CN107018890A (en) | 2017-04-11 | 2017-04-11 | Application of the nylon wire as isolated material on corn and corn Wild related germplasm breeding |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201710231477.4A Pending CN107018890A (en) | 2017-04-11 | 2017-04-11 | Application of the nylon wire as isolated material on corn and corn Wild related germplasm breeding |
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Cited By (1)
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