CN107119014A - 一种nk细胞无动物源培养基 - Google Patents

一种nk细胞无动物源培养基 Download PDF

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CN107119014A
CN107119014A CN201710332391.0A CN201710332391A CN107119014A CN 107119014 A CN107119014 A CN 107119014A CN 201710332391 A CN201710332391 A CN 201710332391A CN 107119014 A CN107119014 A CN 107119014A
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曹殿秀
李民
李一民
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Qingdao Sai'ou Cape Tule Biological Medicine Co Ltd
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Abstract

本发明公开了一种NK细胞无动物源培养基，包括L‑精氨酸、L‑门冬酰胺、L‑门冬氨酸、L‑胱氨酸二盐酸盐、L‑谷氨酸、甘氨酸、L‑组氨酸、L‑羟脯氨酸、L‑亮氨酸、L‑赖氨酸盐酸盐、L‑甲硫氨酸、L‑苯丙氨酸、L‑脯氨酸、L‑丝氨酸、L‑苏氨酸、L‑色氨酸、L‑酪氨酸、L‑缬氨酸、L‑谷氨酰胺、丁二酸钠、丁二酸、丙酮酸钠、对氨基苯甲酸、硝酸钙、硝酸钾、硝酸铵、无水硫酸镁、无水磷酸二氢钠、碳酸氢钠、氯化钾、氯化钠、氯化镁、无水氯化钙、葡萄糖、核糖、维生素E、维生素B12、还原谷胱甘肽、酚红、生物素、D‑泛酸钙、肌醇、叶酸、核黄素、烟酰胺。本发明的有益效果是：1.NK细胞培养无需添加血清，无动物蛋白成分；2、细胞扩增倍数高，细胞活率高。

Description

一种NK细胞无动物源培养基
技术领域
本发明涉及一种细胞培植技术，具体是一种NK细胞无动物源培养基。
背景技术
培养基(Medium)是供微生物、植物组织和动物组织生长和维持用的人工配制的养料，一般都含有碳水化合物、含氮物质、无机盐(包括微量元素)以及维生素和水等。不同培养基可根据实际需要，添加一些自身无法合成的化合物，即生长因子。有些微生物，如自养型微生物，不需要碳源，所以上述物质只具有一般性。
现有的培养基的成分都含有动物蛋白，存在一定的缺陷。
发明内容
本发明的目的在于提供一种NK细胞无动物源培养基，以解决上述背景技术中提出的问题。
为实现上述目的，本发明提供如下技术方案：
一种NK细胞无动物源培养基，包括L-精氨酸、L-门冬酰胺、L-门冬氨酸、L-胱氨酸二盐酸盐、L-谷氨酸、甘氨酸、L-组氨酸、L-羟脯氨酸、L-亮氨酸、L-赖氨酸盐酸盐、L-甲硫氨酸、L-苯丙氨酸、L-脯氨酸、L-丝氨酸、L-苏氨酸、L-色氨酸、L-酪氨酸、L-缬氨酸、L-谷氨酰胺、丁二酸钠、丁二酸、丙酮酸钠、对氨基苯甲酸、硝酸钙、硝酸钾、硝酸铵、无水硫酸镁、无水磷酸二氢钠、碳酸氢钠、氯化钾、氯化钠、氯化镁、无水氯化钙、葡萄糖、核糖、维生素E、维生素B12、还原谷胱甘肽、酚红、生物素、D-泛酸钙、肌醇、叶酸、核黄素、烟酰胺。
作为本发明的优选方案：还包括D-泛酸钙，可选地浓度为1ng/ml至10ng/ml。
作为本发明的优选方案：还包括肌醇，可选地浓度为1ng/ml至20ng/ml。
作为本发明的优选方案：还包括叶酸，可选地浓度为1ng/ml至50ng/ml。
作为本发明的优选方案：还包括核黄素，可选地浓度为5ng/ml至10ng/ml。
作为本发明的优选方案：还包括烟酰胺，可选地浓度为5ng/ml至150ng/ml。
与现有技术相比，本发明的有益效果是：1.NK细胞培养无需添加血清，无动物蛋白成分；2、细胞扩增倍数高，细胞活率高。
具体实施方式
下面将对本发明实施例中的技术方案进行清楚、完整地描述，显然，所描述的实施例仅仅是本发明一部分实施例，而不是全部的实施例。基于本发明中的实施例，本领域普通技术人员在没有做出创造性劳动前提下所获得的所有其他实施例，都属于本发明保护的范围。
本发明实施例中，一种NK细胞无动物源培养基，包括L-精氨酸、L-门冬酰胺、L-门冬氨酸、L-胱氨酸二盐酸盐、L-谷氨酸、甘氨酸、L-组氨酸、L-羟脯氨酸、L-亮氨酸、L-赖氨酸盐酸盐、L-甲硫氨酸、L-苯丙氨酸、L-脯氨酸、L-丝氨酸、L-苏氨酸、L-色氨酸、L-酪氨酸、L-缬氨酸、L-谷氨酰胺、丁二酸钠、丁二酸、丙酮酸钠、对氨基苯甲酸、硝酸钙、硝酸钾、硝酸铵、无水硫酸镁、无水磷酸二氢钠、碳酸氢钠、氯化钾、氯化钠、氯化镁、无水氯化钙、葡萄糖、核糖、维生素E、维生素B12、还原谷胱甘肽、酚红、生物素、D-泛酸钙、肌醇、叶酸、核黄素、烟酰胺。
还包括D-泛酸钙，可选地浓度为1ng/ml至10ng/ml。还包括肌醇，可选地浓度为1ng/ml至20ng/ml。还包括叶酸，可选地浓度为1ng/ml至50ng/ml。还包括核黄素，可选地浓度为5ng/ml至10ng/ml。还包括烟酰胺，可选地浓度为5ng/ml至150ng/ml。

Claims (6)

1.一种NK细胞无动物源培养基，其特征在于，包括L-精氨酸、L-门冬酰胺、L-门冬氨酸、L-胱氨酸二盐酸盐、L-谷氨酸、甘氨酸、L-组氨酸、L-羟脯氨酸、L-亮氨酸、L-赖氨酸盐酸盐、L-甲硫氨酸、L-苯丙氨酸、L-脯氨酸、L-丝氨酸、L-苏氨酸、L-色氨酸、L-酪氨酸、L-缬氨酸、L-谷氨酰胺、丁二酸钠、丁二酸、丙酮酸钠、对氨基苯甲酸、硝酸钙、硝酸钾、硝酸铵、无水硫酸镁、无水磷酸二氢钠、碳酸氢钠、氯化钾、氯化钠、氯化镁、无水氯化钙、葡萄糖、核糖、维生素E、维生素B12、还原谷胱甘肽、酚红、生物素、D-泛酸钙、肌醇、叶酸、核黄素、烟酰胺。
2.根据权利要求1所述的一种NK细胞无动物源培养基，其特征在于，还包括D-泛酸钙，可选地浓度为1ng/ml至10ng/ml。
3.根据权利要求1所述的一种NK细胞无动物源培养基，其特征在于，还包括肌醇，可选地浓度为1ng/ml至20ng/ml。
4.根据权利要求1所述的一种NK细胞无动物源培养基，其特征在于，还包括叶酸，可选地浓度为1ng/ml至50ng/ml。
5.根据权利要求1所述的一种NK细胞无动物源培养基，其特征在于，还包括核黄素，可选地浓度为5ng/ml至10ng/ml。
6.根据权利要求1所述的一种NK细胞无动物源培养基，其特征在于，还包括烟酰胺，可选地浓度为5ng/ml至150ng/ml。
CN201710332391.0A 2017-05-12 2017-05-12 一种nk细胞无动物源培养基 Pending CN107119014A (zh)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113881629A (zh) * 2020-07-03 2022-01-04 山东荆卫生物科技有限公司 一种体外高效扩增nk细胞的培养基及培养方法

Citations (2)

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CN104450614A (zh) * 2014-12-05 2015-03-25 上海安集协康生物技术有限公司 一种无动物蛋白源的免疫细胞无血清培养基及其使用方法
CN105462924A (zh) * 2015-12-04 2016-04-06 广州赛莱拉干细胞科技股份有限公司 Nk细胞的培养方法及无血清培养基组合

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CN104450614A (zh) * 2014-12-05 2015-03-25 上海安集协康生物技术有限公司 一种无动物蛋白源的免疫细胞无血清培养基及其使用方法
CN105462924A (zh) * 2015-12-04 2016-04-06 广州赛莱拉干细胞科技股份有限公司 Nk细胞的培养方法及无血清培养基组合

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113881629A (zh) * 2020-07-03 2022-01-04 山东荆卫生物科技有限公司 一种体外高效扩增nk细胞的培养基及培养方法

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Application publication date: 20170901