CN107109434A - 新颖cho整合位点和其用途 - Google Patents

新颖cho整合位点和其用途 Download PDF

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Publication number
CN107109434A
CN107109434A CN201580069889.1A CN201580069889A CN107109434A CN 107109434 A CN107109434 A CN 107109434A CN 201580069889 A CN201580069889 A CN 201580069889A CN 107109434 A CN107109434 A CN 107109434A
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sequence
seq
site
nucleic acid
goi
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Y·沈
D·巴拉科夫
G·陈
J·P·范德尔
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Regeneron Pharmaceuticals Inc
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Application filed by Regeneron Pharmaceuticals Inc filed Critical Regeneron Pharmaceuticals Inc
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Mycology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Saccharide Compounds (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
CN201580069889.1A 2014-10-23 2015-10-21 新颖cho整合位点和其用途 Pending CN107109434A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201462067774P 2014-10-23 2014-10-23
US62/067,774 2014-10-23
PCT/US2015/056653 WO2016064999A1 (en) 2014-10-23 2015-10-21 Novel cho integration sites and uses thereof

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CN107109434A true CN107109434A (zh) 2017-08-29

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CN201580069889.1A Pending CN107109434A (zh) 2014-10-23 2015-10-21 新颖cho整合位点和其用途

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US (5) US9816110B2 (https=)
EP (1) EP3209785B1 (https=)
JP (1) JP6668340B2 (https=)
KR (1) KR102243243B1 (https=)
CN (1) CN107109434A (https=)
AR (1) AR102420A1 (https=)
AU (1) AU2015335921B2 (https=)
BR (1) BR112017008022A2 (https=)
CA (1) CA2965495C (https=)
EA (1) EA037255B1 (https=)
IL (1) IL251674B (https=)
MX (1) MX2017005306A (https=)
MY (1) MY184103A (https=)
SG (1) SG11201703149RA (https=)
TW (2) TWI780847B (https=)
WO (1) WO2016064999A1 (https=)

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CN107723276A (zh) * 2017-11-02 2018-02-23 上海交通大学 一种稳定高表达目标产物的细胞株的构建方法和试剂盒
CN109071633A (zh) * 2016-04-20 2018-12-21 瑞泽恩制药公司 基于使用表达增强性基因座来制备抗体的组合物和方法
CN109136193A (zh) * 2018-10-30 2019-01-04 江南大学 一种cho细胞基因组内nw_006884764-1稳定表达蛋白质的应用
CN109207432A (zh) * 2018-10-30 2019-01-15 江南大学 一种cho细胞基因组内nw_006883358-1稳定表达蛋白质的应用
CN109295092A (zh) * 2018-10-30 2019-02-01 江南大学 一种cho细胞基因组内nw_003613638-1稳定表达蛋白质的应用
CN109295093A (zh) * 2018-10-30 2019-02-01 江南大学 一种cho细胞基因组内nw_006882456-1稳定表达蛋白质的应用
CN109321604A (zh) * 2018-10-30 2019-02-12 江南大学 一种cho细胞基因组内nw_006882077-1稳定表达蛋白质的应用
CN109337927A (zh) * 2018-10-30 2019-02-15 江南大学 一种cho细胞基因组内nw_006880285-1稳定表达蛋白质的应用
CN111566208A (zh) * 2017-12-22 2020-08-21 生物辐射实验室股份有限公司 利用CRISPR/Cas基因靶向控制生物体表型
CN111886244A (zh) * 2017-12-22 2020-11-03 豪夫迈·罗氏有限公司 核酸的靶向整合
CN113631711A (zh) * 2018-12-21 2021-11-09 豪夫迈·罗氏有限公司 核酸的靶向整合
CN113755447A (zh) * 2021-09-23 2021-12-07 云舟生物科技（广州）有限公司 一种用于生产腺病毒的293a细胞株及其制备与应用
CN116515757A (zh) * 2016-04-20 2023-08-01 瑞泽恩制药公司 基于使用表达增强性基因座来制备抗体的组合物和方法
WO2023246626A1 (zh) * 2022-06-21 2023-12-28 深圳太力生物技术有限责任公司 靶向整合细胞及其制备方法、生产目标基因表达产物的方法

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BR112017008022A2 (pt) 2014-10-23 2018-02-20 Regeneron Pharmaceuticals, Inc. célula, polinucleotídeo, veículo, e, métodos para modificação de um genoma de células cho e para preparação de uma proteína de interesse.
US11649449B2 (en) 2015-09-03 2023-05-16 CHO Plus, Inc. Hybrid cell lines for high level production of a target protein
US10329594B1 (en) 2015-09-03 2019-06-25 CHO Plus, Inc. Cell lines for high level production of protein-based pharmaceuticals
EP3504328A1 (en) 2016-08-24 2019-07-03 Regeneron Pharmaceuticals, Inc. Host cell protein modification
JP2020501593A (ja) * 2016-12-20 2020-01-23 ディベロップメント センター フォー バイオテクノロジーＤｅｖｅｌｏｐｍｅｎｔ Ｃｅｎｔｅｒ Ｆｏｒ Ｂｉｏｔｅｃｈｎｏｌｏｇｙ 改変細胞、調製方法、及び構築物
US20210309988A1 (en) * 2017-02-07 2021-10-07 Sigma-Aldrich Co. Llc Stable targeted integration
KR102719587B1 (ko) * 2017-02-17 2024-10-17 론자 리미티드 아데노-관련 바이러스 생산을 위한 포유동물 세포
EP3601542A4 (en) * 2017-03-19 2021-01-13 Applied Stemcell, Inc. NEW INTEGRATION SITES AND THEIR USES
CA3068779A1 (en) 2017-08-11 2019-02-14 Boehringer Ingelheim International Gmbh Integration sites in cho cells
WO2020084034A1 (en) 2018-10-26 2020-04-30 F. Hoffmann-La Roche Ag Multispecific antibody screening method using recombinase mediated cassette exchange
JP7522100B2 (ja) * 2019-04-02 2024-07-24 中外製薬株式会社 標的特異的な外来遺伝子の導入方法
AU2020308002A1 (en) 2019-06-26 2022-01-20 Genentech, Inc. Randomized configuration targeted integration of nucleic acids
EP3901266A1 (en) * 2020-04-22 2021-10-27 LEK Pharmaceuticals d.d. Super-enhancers for recombinant gene expression in cho cells
WO2021247672A1 (en) * 2020-06-02 2021-12-09 Catalent Pharma Solutions, Llc Nucleic acid constructs for protein manufacture
JP2023532665A (ja) * 2020-06-24 2023-07-31 ジェネンテック， インコーポレイテッド 核酸の標的化組み込み
CA3197730A1 (en) 2020-10-15 2022-04-21 F. Hoffman-La Roche Ag Nucleic acid constructs for va rna transcription
CA3235566A1 (en) 2021-10-18 2023-04-27 Michael Goren Mammalian cells comprising integrated cas9 genes to produce stable integration sites, and mammalian cells comprising stable integration sites and other sites
CN118159660A (zh) 2021-10-18 2024-06-07 瑞泽恩制药公司 多核苷酸的受控转录
TW202323529A (zh) 2021-10-18 2023-06-16 美商再生元醫藥公司 包括腺病毒相關病毒聚核苷酸的真核細胞
KR20240139051A (ko) * 2022-01-28 2024-09-20 더 트러스티스 오브 콜롬비아 유니버시티 인 더 시티 오브 뉴욕 이종이식을 위한 유전 변형
WO2024107452A1 (en) 2022-11-18 2024-05-23 Regeneron Pharmaceuticals, Inc. Methods for detecting and determining protein structures and stability in fluids, including biological fluids
CN118726475B (zh) * 2023-03-28 2025-07-08 深圳太力生物技术有限责任公司 核酸、重组载体、靶向整合细胞、基因表达方法和应用
WO2024211287A1 (en) * 2023-04-03 2024-10-10 Seagen Inc. Production cell lines with targeted integration sites
TW202528546A (zh) 2023-09-07 2025-07-16 美商再生元醫藥公司 經共價表面修飾之腺相關病毒的生產及純化
TW202540433A (zh) 2023-11-21 2025-10-16 美商再生元醫藥公司 藉由活體外接合產生共價表面修飾之腺相關病毒及共價表面修飾的腺相關病毒之純化
WO2026050618A1 (en) 2024-08-29 2026-03-05 Regeneron Pharmaceuticals, Inc. Polynucleotide constructs and cell lines for recombinant aav production

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Cited By (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109071633A (zh) * 2016-04-20 2018-12-21 瑞泽恩制药公司 基于使用表达增强性基因座来制备抗体的组合物和方法
CN116515757A (zh) * 2016-04-20 2023-08-01 瑞泽恩制药公司 基于使用表达增强性基因座来制备抗体的组合物和方法
CN109071633B (zh) * 2016-04-20 2022-11-18 瑞泽恩制药公司 基于使用表达增强性基因座来制备抗体的组合物和方法
CN107723276A (zh) * 2017-11-02 2018-02-23 上海交通大学 一种稳定高表达目标产物的细胞株的构建方法和试剂盒
CN107723276B (zh) * 2017-11-02 2021-08-13 上海交通大学 一种稳定高表达目标产物的细胞株的构建方法和试剂盒
CN111566208A (zh) * 2017-12-22 2020-08-21 生物辐射实验室股份有限公司 利用CRISPR/Cas基因靶向控制生物体表型
CN111886244A (zh) * 2017-12-22 2020-11-03 豪夫迈·罗氏有限公司 核酸的靶向整合
CN109295092A (zh) * 2018-10-30 2019-02-01 江南大学 一种cho细胞基因组内nw_003613638-1稳定表达蛋白质的应用
CN109337927A (zh) * 2018-10-30 2019-02-15 江南大学 一种cho细胞基因组内nw_006880285-1稳定表达蛋白质的应用
CN109321604A (zh) * 2018-10-30 2019-02-12 江南大学 一种cho细胞基因组内nw_006882077-1稳定表达蛋白质的应用
CN109321604B (zh) * 2018-10-30 2021-07-06 江南大学 一种cho细胞基因组内nw_006882077-1稳定表达蛋白质的应用
CN109136193B (zh) * 2018-10-30 2021-07-06 江南大学 一种cho细胞基因组内nw_006884764-1稳定表达蛋白质的应用
CN109295093B (zh) * 2018-10-30 2021-08-03 江南大学 一种cho细胞基因组内nw_006882456-1稳定表达蛋白质的应用
CN109295093A (zh) * 2018-10-30 2019-02-01 江南大学 一种cho细胞基因组内nw_006882456-1稳定表达蛋白质的应用
CN109207432A (zh) * 2018-10-30 2019-01-15 江南大学 一种cho细胞基因组内nw_006883358-1稳定表达蛋白质的应用
CN109136193A (zh) * 2018-10-30 2019-01-04 江南大学 一种cho细胞基因组内nw_006884764-1稳定表达蛋白质的应用
CN113631711A (zh) * 2018-12-21 2021-11-09 豪夫迈·罗氏有限公司 核酸的靶向整合
CN113755447A (zh) * 2021-09-23 2021-12-07 云舟生物科技（广州）有限公司 一种用于生产腺病毒的293a细胞株及其制备与应用
WO2023246626A1 (zh) * 2022-06-21 2023-12-28 深圳太力生物技术有限责任公司 靶向整合细胞及其制备方法、生产目标基因表达产物的方法

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US11268109B2 (en) 2022-03-08
AU2015335921B2 (en) 2020-06-25
US11788102B2 (en) 2023-10-17
US12565667B2 (en) 2026-03-03
MY184103A (en) 2021-03-18
TWI780847B (zh) 2022-10-11
US20210171984A1 (en) 2021-06-10
SG11201703149RA (en) 2018-02-27
KR102243243B1 (ko) 2021-04-22
JP2017535258A (ja) 2017-11-30
CA2965495A1 (en) 2016-04-28
IL251674B (en) 2020-10-29
WO2016064999A1 (en) 2016-04-28
TW202144576A (zh) 2021-12-01
CA2965495C (en) 2022-05-24
US9816110B2 (en) 2017-11-14
US20200002732A1 (en) 2020-01-02
AR102420A1 (es) 2017-03-01
EA201790698A1 (ru) 2018-01-31
EP3209785A1 (en) 2017-08-30
US20240018553A1 (en) 2024-01-18
TW201629229A (zh) 2016-08-16
KR20170096999A (ko) 2017-08-25
US20160115502A1 (en) 2016-04-28
BR112017008022A2 (pt) 2018-02-20
MX2017005306A (es) 2018-01-09
JP6668340B2 (ja) 2020-03-18
US20180030480A1 (en) 2018-02-01
EA037255B1 (ru) 2021-02-26
AU2015335921A1 (en) 2017-05-04
EP3209785B1 (en) 2019-07-31
IL251674A0 (en) 2017-06-29
TWI747808B (zh) 2021-12-01

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