TWI780847B - 新穎之ｃｈｏ整合位點及其用途 - Google Patents

新穎之ｃｈｏ整合位點及其用途 Download PDF

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TWI780847B
TWI780847B TW110128495A TW110128495A TWI780847B TW I780847 B TWI780847 B TW I780847B TW 110128495 A TW110128495 A TW 110128495A TW 110128495 A TW110128495 A TW 110128495A TW I780847 B TWI780847 B TW I780847B
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fluorescent protein
seq
cell
gene
selectable marker
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TW110128495A
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TW202144576A (zh
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沈盈
達里亞 布拉科夫
剛 陳
詹姆斯 凡德爾
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美商再生元醫藥公司
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells

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  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
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  • Polysaccharides And Polysaccharide Derivatives (AREA)
TW110128495A 2014-10-23 2015-10-22 新穎之ｃｈｏ整合位點及其用途 TWI780847B (zh)

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US201462067774P 2014-10-23 2014-10-23
US62/067,774 2014-10-23

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TW202144576A TW202144576A (zh) 2021-12-01
TWI780847B true TWI780847B (zh) 2022-10-11

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TW110128495A TWI780847B (zh) 2014-10-23 2015-10-22 新穎之ｃｈｏ整合位點及其用途
TW104134716A TWI747808B (zh) 2014-10-23 2015-10-22 新穎之ｃｈｏ整合位點及其用途

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US (5) US9816110B2 (https=)
EP (1) EP3209785B1 (https=)
JP (1) JP6668340B2 (https=)
KR (1) KR102243243B1 (https=)
CN (1) CN107109434A (https=)
AR (1) AR102420A1 (https=)
AU (1) AU2015335921B2 (https=)
BR (1) BR112017008022A2 (https=)
CA (1) CA2965495C (https=)
EA (1) EA037255B1 (https=)
IL (1) IL251674B (https=)
MX (1) MX2017005306A (https=)
MY (1) MY184103A (https=)
SG (1) SG11201703149RA (https=)
TW (2) TWI780847B (https=)
WO (1) WO2016064999A1 (https=)

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SG11201807885PA (en) 2016-04-20 2018-10-30 Regeneron Pharma Compositions and methods for making antibodies based on use of expression-enhancing loci
EP3504328A1 (en) 2016-08-24 2019-07-03 Regeneron Pharmaceuticals, Inc. Host cell protein modification
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US20210309988A1 (en) * 2017-02-07 2021-10-07 Sigma-Aldrich Co. Llc Stable targeted integration
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CN107723276B (zh) * 2017-11-02 2021-08-13 上海交通大学 一种稳定高表达目标产物的细胞株的构建方法和试剂盒
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WO2020084034A1 (en) 2018-10-26 2020-04-30 F. Hoffmann-La Roche Ag Multispecific antibody screening method using recombinase mediated cassette exchange
CN109207432B (zh) * 2018-10-30 2021-08-03 江南大学 一种cho细胞基因组内nw_006883358-1稳定表达蛋白质的应用
CN109295093B (zh) * 2018-10-30 2021-08-03 江南大学 一种cho细胞基因组内nw_006882456-1稳定表达蛋白质的应用
CN109295092B (zh) * 2018-10-30 2021-06-29 江南大学 一种cho细胞基因组内nw_003613638-1稳定表达蛋白质的应用
CN109337927B (zh) * 2018-10-30 2021-07-06 江南大学 一种cho细胞基因组内nw_006880285-1稳定表达蛋白质的应用
CN109321604B (zh) * 2018-10-30 2021-07-06 江南大学 一种cho细胞基因组内nw_006882077-1稳定表达蛋白质的应用
CN109136193B (zh) * 2018-10-30 2021-07-06 江南大学 一种cho细胞基因组内nw_006884764-1稳定表达蛋白质的应用
AU2019403279A1 (en) * 2018-12-21 2021-07-01 Genentech, Inc. Targeted integration of nucleic acids
JP7522100B2 (ja) * 2019-04-02 2024-07-24 中外製薬株式会社 標的特異的な外来遺伝子の導入方法
AU2020308002A1 (en) 2019-06-26 2022-01-20 Genentech, Inc. Randomized configuration targeted integration of nucleic acids
EP3901266A1 (en) * 2020-04-22 2021-10-27 LEK Pharmaceuticals d.d. Super-enhancers for recombinant gene expression in cho cells
WO2021247672A1 (en) * 2020-06-02 2021-12-09 Catalent Pharma Solutions, Llc Nucleic acid constructs for protein manufacture
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CA3197730A1 (en) 2020-10-15 2022-04-21 F. Hoffman-La Roche Ag Nucleic acid constructs for va rna transcription
CN113755447B (zh) * 2021-09-23 2022-04-29 云舟生物科技(广州)股份有限公司 一种用于生产腺病毒的293a细胞株及其制备与应用
CA3235566A1 (en) 2021-10-18 2023-04-27 Michael Goren Mammalian cells comprising integrated cas9 genes to produce stable integration sites, and mammalian cells comprising stable integration sites and other sites
CN118159660A (zh) 2021-10-18 2024-06-07 瑞泽恩制药公司 多核苷酸的受控转录
TW202323529A (zh) 2021-10-18 2023-06-16 美商再生元醫藥公司 包括腺病毒相關病毒聚核苷酸的真核細胞
KR20240139051A (ko) * 2022-01-28 2024-09-20 더 트러스티스 오브 콜롬비아 유니버시티 인 더 시티 오브 뉴욕 이종이식을 위한 유전 변형
CN117305334B (zh) * 2022-06-21 2024-11-08 深圳太力生物技术有限责任公司 靶向整合细胞及其制备方法、生产目标基因表达产物的方法
WO2024107452A1 (en) 2022-11-18 2024-05-23 Regeneron Pharmaceuticals, Inc. Methods for detecting and determining protein structures and stability in fluids, including biological fluids
CN118726475B (zh) * 2023-03-28 2025-07-08 深圳太力生物技术有限责任公司 核酸、重组载体、靶向整合细胞、基因表达方法和应用
WO2024211287A1 (en) * 2023-04-03 2024-10-10 Seagen Inc. Production cell lines with targeted integration sites
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TW202540433A (zh) 2023-11-21 2025-10-16 美商再生元醫藥公司 藉由活體外接合產生共價表面修飾之腺相關病毒及共價表面修飾的腺相關病毒之純化
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US11268109B2 (en) 2022-03-08
AU2015335921B2 (en) 2020-06-25
US11788102B2 (en) 2023-10-17
US12565667B2 (en) 2026-03-03
MY184103A (en) 2021-03-18
US20210171984A1 (en) 2021-06-10
SG11201703149RA (en) 2018-02-27
KR102243243B1 (ko) 2021-04-22
JP2017535258A (ja) 2017-11-30
CA2965495A1 (en) 2016-04-28
IL251674B (en) 2020-10-29
WO2016064999A1 (en) 2016-04-28
TW202144576A (zh) 2021-12-01
CA2965495C (en) 2022-05-24
US9816110B2 (en) 2017-11-14
US20200002732A1 (en) 2020-01-02
AR102420A1 (es) 2017-03-01
EA201790698A1 (ru) 2018-01-31
EP3209785A1 (en) 2017-08-30
US20240018553A1 (en) 2024-01-18
TW201629229A (zh) 2016-08-16
KR20170096999A (ko) 2017-08-25
US20160115502A1 (en) 2016-04-28
BR112017008022A2 (pt) 2018-02-20
MX2017005306A (es) 2018-01-09
JP6668340B2 (ja) 2020-03-18
CN107109434A (zh) 2017-08-29
US20180030480A1 (en) 2018-02-01
EA037255B1 (ru) 2021-02-26
AU2015335921A1 (en) 2017-05-04
EP3209785B1 (en) 2019-07-31
IL251674A0 (en) 2017-06-29
TWI747808B (zh) 2021-12-01

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