KR102243243B1 - 신규한 cho 통합 부위 및 이의 용도 - Google Patents

신규한 cho 통합 부위 및 이의 용도 Download PDF

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KR102243243B1
KR102243243B1 KR1020177012465A KR20177012465A KR102243243B1 KR 102243243 B1 KR102243243 B1 KR 102243243B1 KR 1020177012465 A KR1020177012465 A KR 1020177012465A KR 20177012465 A KR20177012465 A KR 20177012465A KR 102243243 B1 KR102243243 B1 KR 102243243B1
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KR20170096999A (ko
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잉 셴
다리야 부라코프
강 첸
제임스 피. 팬들
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리제너론 파마슈티칼스 인코포레이티드
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells

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  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
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KR1020177012465A 2014-10-23 2015-10-21 신규한 cho 통합 부위 및 이의 용도 Active KR102243243B1 (ko)

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Application Number Priority Date Filing Date Title
US201462067774P 2014-10-23 2014-10-23
US62/067,774 2014-10-23
PCT/US2015/056653 WO2016064999A1 (en) 2014-10-23 2015-10-21 Novel cho integration sites and uses thereof

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KR20170096999A KR20170096999A (ko) 2017-08-25
KR102243243B1 true KR102243243B1 (ko) 2021-04-22

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US (5) US9816110B2 (https=)
EP (1) EP3209785B1 (https=)
JP (1) JP6668340B2 (https=)
KR (1) KR102243243B1 (https=)
CN (1) CN107109434A (https=)
AR (1) AR102420A1 (https=)
AU (1) AU2015335921B2 (https=)
BR (1) BR112017008022A2 (https=)
CA (1) CA2965495C (https=)
EA (1) EA037255B1 (https=)
IL (1) IL251674B (https=)
MX (1) MX2017005306A (https=)
MY (1) MY184103A (https=)
SG (1) SG11201703149RA (https=)
TW (2) TWI780847B (https=)
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US11649449B2 (en) 2015-09-03 2023-05-16 CHO Plus, Inc. Hybrid cell lines for high level production of a target protein
US10329594B1 (en) 2015-09-03 2019-06-25 CHO Plus, Inc. Cell lines for high level production of protein-based pharmaceuticals
BR112018071285A2 (pt) 2016-04-20 2019-02-12 Regeneron Pharma célula, conjunto de vetores, vetor, sistema, e, método
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EP3504328A1 (en) 2016-08-24 2019-07-03 Regeneron Pharmaceuticals, Inc. Host cell protein modification
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US20210309988A1 (en) * 2017-02-07 2021-10-07 Sigma-Aldrich Co. Llc Stable targeted integration
KR102719587B1 (ko) * 2017-02-17 2024-10-17 론자 리미티드 아데노-관련 바이러스 생산을 위한 포유동물 세포
EP3601542A4 (en) * 2017-03-19 2021-01-13 Applied Stemcell, Inc. NEW INTEGRATION SITES AND THEIR USES
CA3068779A1 (en) 2017-08-11 2019-02-14 Boehringer Ingelheim International Gmbh Integration sites in cho cells
CN107723276B (zh) * 2017-11-02 2021-08-13 上海交通大学 一种稳定高表达目标产物的细胞株的构建方法和试剂盒
US20200318106A1 (en) * 2017-12-22 2020-10-08 Bio-Rad Laboratories, Inc. Controlling phenotype of organisms with crispr/cas gene targeting
IL275462B2 (en) * 2017-12-22 2026-01-01 Genentech Inc Targeted integration of nucleic acids
WO2020084034A1 (en) 2018-10-26 2020-04-30 F. Hoffmann-La Roche Ag Multispecific antibody screening method using recombinase mediated cassette exchange
CN109207432B (zh) * 2018-10-30 2021-08-03 江南大学 一种cho细胞基因组内nw_006883358-1稳定表达蛋白质的应用
CN109295093B (zh) * 2018-10-30 2021-08-03 江南大学 一种cho细胞基因组内nw_006882456-1稳定表达蛋白质的应用
CN109295092B (zh) * 2018-10-30 2021-06-29 江南大学 一种cho细胞基因组内nw_003613638-1稳定表达蛋白质的应用
CN109337927B (zh) * 2018-10-30 2021-07-06 江南大学 一种cho细胞基因组内nw_006880285-1稳定表达蛋白质的应用
CN109321604B (zh) * 2018-10-30 2021-07-06 江南大学 一种cho细胞基因组内nw_006882077-1稳定表达蛋白质的应用
CN109136193B (zh) * 2018-10-30 2021-07-06 江南大学 一种cho细胞基因组内nw_006884764-1稳定表达蛋白质的应用
AU2019403279A1 (en) * 2018-12-21 2021-07-01 Genentech, Inc. Targeted integration of nucleic acids
JP7522100B2 (ja) * 2019-04-02 2024-07-24 中外製薬株式会社 標的特異的な外来遺伝子の導入方法
AU2020308002A1 (en) 2019-06-26 2022-01-20 Genentech, Inc. Randomized configuration targeted integration of nucleic acids
EP3901266A1 (en) * 2020-04-22 2021-10-27 LEK Pharmaceuticals d.d. Super-enhancers for recombinant gene expression in cho cells
WO2021247672A1 (en) * 2020-06-02 2021-12-09 Catalent Pharma Solutions, Llc Nucleic acid constructs for protein manufacture
JP2023532665A (ja) * 2020-06-24 2023-07-31 ジェネンテック， インコーポレイテッド 核酸の標的化組み込み
CA3197730A1 (en) 2020-10-15 2022-04-21 F. Hoffman-La Roche Ag Nucleic acid constructs for va rna transcription
CN113755447B (zh) * 2021-09-23 2022-04-29 云舟生物科技(广州)股份有限公司 一种用于生产腺病毒的293a细胞株及其制备与应用
CA3235566A1 (en) 2021-10-18 2023-04-27 Michael Goren Mammalian cells comprising integrated cas9 genes to produce stable integration sites, and mammalian cells comprising stable integration sites and other sites
CN118159660A (zh) 2021-10-18 2024-06-07 瑞泽恩制药公司 多核苷酸的受控转录
TW202323529A (zh) 2021-10-18 2023-06-16 美商再生元醫藥公司 包括腺病毒相關病毒聚核苷酸的真核細胞
KR20240139051A (ko) * 2022-01-28 2024-09-20 더 트러스티스 오브 콜롬비아 유니버시티 인 더 시티 오브 뉴욕 이종이식을 위한 유전 변형
CN117305334B (zh) * 2022-06-21 2024-11-08 深圳太力生物技术有限责任公司 靶向整合细胞及其制备方法、生产目标基因表达产物的方法
WO2024107452A1 (en) 2022-11-18 2024-05-23 Regeneron Pharmaceuticals, Inc. Methods for detecting and determining protein structures and stability in fluids, including biological fluids
CN118726475B (zh) * 2023-03-28 2025-07-08 深圳太力生物技术有限责任公司 核酸、重组载体、靶向整合细胞、基因表达方法和应用
WO2024211287A1 (en) * 2023-04-03 2024-10-10 Seagen Inc. Production cell lines with targeted integration sites
TW202528546A (zh) 2023-09-07 2025-07-16 美商再生元醫藥公司 經共價表面修飾之腺相關病毒的生產及純化
TW202540433A (zh) 2023-11-21 2025-10-16 美商再生元醫藥公司 藉由活體外接合產生共價表面修飾之腺相關病毒及共價表面修飾的腺相關病毒之純化
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US11268109B2 (en) 2022-03-08
AU2015335921B2 (en) 2020-06-25
US11788102B2 (en) 2023-10-17
US12565667B2 (en) 2026-03-03
MY184103A (en) 2021-03-18
TWI780847B (zh) 2022-10-11
US20210171984A1 (en) 2021-06-10
SG11201703149RA (en) 2018-02-27
JP2017535258A (ja) 2017-11-30
CA2965495A1 (en) 2016-04-28
IL251674B (en) 2020-10-29
WO2016064999A1 (en) 2016-04-28
TW202144576A (zh) 2021-12-01
CA2965495C (en) 2022-05-24
US9816110B2 (en) 2017-11-14
US20200002732A1 (en) 2020-01-02
AR102420A1 (es) 2017-03-01
EA201790698A1 (ru) 2018-01-31
EP3209785A1 (en) 2017-08-30
US20240018553A1 (en) 2024-01-18
TW201629229A (zh) 2016-08-16
KR20170096999A (ko) 2017-08-25
US20160115502A1 (en) 2016-04-28
BR112017008022A2 (pt) 2018-02-20
MX2017005306A (es) 2018-01-09
JP6668340B2 (ja) 2020-03-18
CN107109434A (zh) 2017-08-29
US20180030480A1 (en) 2018-02-01
EA037255B1 (ru) 2021-02-26
AU2015335921A1 (en) 2017-05-04
EP3209785B1 (en) 2019-07-31
IL251674A0 (en) 2017-06-29
TWI747808B (zh) 2021-12-01

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