CN107109380A - 经修饰的酶 - Google Patents

经修饰的酶 Download PDF

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Publication number
CN107109380A
CN107109380A CN201580066254.6A CN201580066254A CN107109380A CN 107109380 A CN107109380 A CN 107109380A CN 201580066254 A CN201580066254 A CN 201580066254A CN 107109380 A CN107109380 A CN 107109380A
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CN
China
Prior art keywords
unwindase
seq
amino acid
helicase
polynucleotide
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201580066254.6A
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English (en)
Chinese (zh)
Inventor
安德鲁·海伦
瑞贝卡·维多利亚·鲍恩
马克·布鲁斯
拉科马·贾亚辛格
约瑟夫·哈格里夫·劳埃德
绍博尔奇·苏罗尔斯
伊丽莎白·杰恩·华莱士
克里斯多夫·彼得·约德
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Oxford Nanopore Technologies PLC
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Oxford Nanopore Technologies PLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from PCT/GB2015/051291 external-priority patent/WO2015166276A1/en
Application filed by Oxford Nanopore Technologies PLC filed Critical Oxford Nanopore Technologies PLC
Priority to CN202310481940.6A priority Critical patent/CN117089536A/zh
Priority to CN202310481939.3A priority patent/CN116622671A/zh
Publication of CN107109380A publication Critical patent/CN107109380A/zh
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y306/00—Hydrolases acting on acid anhydrides (3.6)
    • C12Y306/04—Hydrolases acting on acid anhydrides (3.6) acting on acid anhydrides; involved in cellular and subcellular movement (3.6.4)
    • C12Y306/04012—DNA helicase (3.6.4.12)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/50—Other enzymatic activities
    • C12Q2521/513—Winding/unwinding enzyme, e.g. helicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/60—Detection means characterised by use of a special device
    • C12Q2565/631—Detection means characterised by use of a special device being a biochannel or pore

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
CN201580066254.6A 2014-10-07 2015-10-06 经修饰的酶 Pending CN107109380A (zh)

Priority Applications (2)

Application Number Priority Date Filing Date Title
CN202310481940.6A CN117089536A (zh) 2014-10-07 2015-10-06 经修饰的酶
CN202310481939.3A CN116622671A (zh) 2014-10-07 2015-10-06 经修饰的酶

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
GB1417712.5 2014-10-07
GBGB1417712.5A GB201417712D0 (en) 2014-10-07 2014-10-07 Method
GBPCT/GB2015/051291 2015-05-01
PCT/GB2015/051291 WO2015166276A1 (en) 2014-05-02 2015-05-01 Method of improving the movement of a target polynucleotide with respect to a transmembrane pore
PCT/GB2015/052916 WO2016055777A2 (en) 2014-10-07 2015-10-06 Modified enzymes

Related Child Applications (2)

Application Number Title Priority Date Filing Date
CN202310481940.6A Division CN117089536A (zh) 2014-10-07 2015-10-06 经修饰的酶
CN202310481939.3A Division CN116622671A (zh) 2014-10-07 2015-10-06 经修饰的酶

Publications (1)

Publication Number Publication Date
CN107109380A true CN107109380A (zh) 2017-08-29

Family

ID=51946970

Family Applications (3)

Application Number Title Priority Date Filing Date
CN202310481940.6A Pending CN117089536A (zh) 2014-10-07 2015-10-06 经修饰的酶
CN202310481939.3A Pending CN116622671A (zh) 2014-10-07 2015-10-06 经修饰的酶
CN201580066254.6A Pending CN107109380A (zh) 2014-10-07 2015-10-06 经修饰的酶

Family Applications Before (2)

Application Number Title Priority Date Filing Date
CN202310481940.6A Pending CN117089536A (zh) 2014-10-07 2015-10-06 经修饰的酶
CN202310481939.3A Pending CN116622671A (zh) 2014-10-07 2015-10-06 经修饰的酶

Country Status (7)

Country Link
US (3) US11180741B2 (enExample)
EP (2) EP4108679A1 (enExample)
JP (1) JP6843042B2 (enExample)
KR (1) KR102436445B1 (enExample)
CN (3) CN117089536A (enExample)
GB (1) GB201417712D0 (enExample)
WO (1) WO2016055777A2 (enExample)

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CN108101962A (zh) * 2017-11-10 2018-06-01 浙江美保龙生物技术有限公司 一种特异性提高猪繁殖与呼吸综合征疫苗免疫效果的小分子多肽及其应用
CN110004074A (zh) * 2019-02-28 2019-07-12 北京蛋白质组研究中心 一种利用silac标记耻垢分枝杆菌蛋白质的方法及其专用培养基
CN112079903A (zh) * 2019-06-14 2020-12-15 中国科学院青岛生物能源与过程研究所 一种错配结合蛋白的突变体及其编码基因
WO2021253410A1 (zh) 2020-06-19 2021-12-23 北京齐碳科技有限公司 一种Pif1-like解旋酶及其应用
CN113930406A (zh) * 2021-12-17 2022-01-14 北京齐碳科技有限公司 一种Pif1-like解旋酶及其应用
WO2022126304A1 (zh) * 2020-12-14 2022-06-23 北京齐碳科技有限公司 一种经修饰的解旋酶及其应用
CN116194117A (zh) * 2020-09-22 2023-05-30 Bp基因有限公司 编码5'-核苷酸酶修饰蛋白的癌症治疗用多核苷酸
CN117384867A (zh) * 2022-09-16 2024-01-12 北京普译生物科技有限公司 一种经修饰的Cas3移位酶及其应用
WO2024056038A1 (zh) * 2022-09-16 2024-03-21 北京普译生物科技有限公司 一种经修饰的CaPif1解旋酶及其应用
CN117964713A (zh) * 2024-02-26 2024-05-03 江苏省疾病预防控制中心(江苏省公共卫生研究院) 一种t4gp32蛋白突变体及其应用
WO2024138574A1 (zh) * 2022-12-29 2024-07-04 深圳华大生命科学研究院 解旋酶及其应用
WO2024138422A1 (zh) * 2022-12-28 2024-07-04 深圳华大生命科学研究院 一种销结构域、含其的解旋酶及制备方法和应用
WO2024138635A1 (zh) * 2022-12-30 2024-07-04 深圳华大生命科学研究院 一种解旋酶及其制备方法和在高通量测序中的应用
WO2024138701A1 (zh) * 2022-12-30 2024-07-04 深圳华大生命科学研究院 一种解旋酶突变体及其制备方法和在高通量测序中的应用
CN119192309A (zh) * 2024-10-25 2024-12-27 新镁(上海)生物技术有限公司 高耐盐单链结合蛋白突变体及其应用
WO2025067293A1 (zh) * 2023-09-27 2025-04-03 北京齐碳科技有限公司 解旋酶及其应用

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CN118355130A (zh) * 2021-12-31 2024-07-16 深圳华大生命科学研究院 解旋酶bch1x及其用途
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