JP6843042B2 - 修飾酵素 - Google Patents
修飾酵素 Download PDFInfo
- Publication number
- JP6843042B2 JP6843042B2 JP2017518977A JP2017518977A JP6843042B2 JP 6843042 B2 JP6843042 B2 JP 6843042B2 JP 2017518977 A JP2017518977 A JP 2017518977A JP 2017518977 A JP2017518977 A JP 2017518977A JP 6843042 B2 JP6843042 B2 JP 6843042B2
- Authority
- JP
- Japan
- Prior art keywords
- helicase
- seq
- polynucleotide
- amino acid
- histidine
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y306/00—Hydrolases acting on acid anhydrides (3.6)
- C12Y306/04—Hydrolases acting on acid anhydrides (3.6) acting on acid anhydrides; involved in cellular and subcellular movement (3.6.4)
- C12Y306/04012—DNA helicase (3.6.4.12)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2521/00—Reaction characterised by the enzymatic activity
- C12Q2521/50—Other enzymatic activities
- C12Q2521/513—Winding/unwinding enzyme, e.g. helicase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
- C12Q2565/60—Detection means characterised by use of a special device
- C12Q2565/631—Detection means characterised by use of a special device being a biochannel or pore
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Immunology (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Biomedical Technology (AREA)
- Medicinal Chemistry (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB1417712.5 | 2014-10-07 | ||
| GBGB1417712.5A GB201417712D0 (en) | 2014-10-07 | 2014-10-07 | Method |
| GBPCT/GB2015/051291 | 2015-05-01 | ||
| PCT/GB2015/051291 WO2015166276A1 (en) | 2014-05-02 | 2015-05-01 | Method of improving the movement of a target polynucleotide with respect to a transmembrane pore |
| PCT/GB2015/052916 WO2016055777A2 (en) | 2014-10-07 | 2015-10-06 | Modified enzymes |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2017535256A JP2017535256A (ja) | 2017-11-30 |
| JP2017535256A5 JP2017535256A5 (enExample) | 2018-11-15 |
| JP6843042B2 true JP6843042B2 (ja) | 2021-03-17 |
Family
ID=51946970
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2017518977A Active JP6843042B2 (ja) | 2014-10-07 | 2015-10-06 | 修飾酵素 |
Country Status (7)
| Country | Link |
|---|---|
| US (3) | US11180741B2 (enExample) |
| EP (2) | EP3204406B1 (enExample) |
| JP (1) | JP6843042B2 (enExample) |
| KR (1) | KR102436445B1 (enExample) |
| CN (3) | CN116622671A (enExample) |
| GB (1) | GB201417712D0 (enExample) |
| WO (1) | WO2016055777A2 (enExample) |
Families Citing this family (72)
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| AU2012264497B2 (en) | 2011-05-27 | 2017-06-15 | Oxford Nanopore Technologies Limited | Coupling method |
| JP6298404B2 (ja) | 2011-07-25 | 2018-03-20 | オックスフォード ナノポール テクノロジーズ リミテッド | 膜貫通ポアを用いる二重鎖ポリヌクレオチド配列決定のためのヘアピンループ方法 |
| JP6226869B2 (ja) | 2011-10-21 | 2017-11-08 | オックスフォード ナノポール テクノロジーズ リミテッド | 酵素法 |
| KR102086182B1 (ko) | 2011-12-29 | 2020-03-06 | 옥스포드 나노포어 테크놀로지즈 리미티드 | 효소 방법 |
| EP2875154B1 (en) | 2012-07-19 | 2017-08-23 | Oxford Nanopore Technologies Limited | SSB method for characterising a nucleic acid |
| AU2013291763B2 (en) | 2012-07-19 | 2019-06-20 | Oxford Nanopore Technologies Limited | Modified helicases |
| AU2014224432B2 (en) | 2013-03-08 | 2019-10-24 | Oxford Nanopore Technologies Limited | Enzyme stalling method |
| CA2927726A1 (en) | 2013-10-18 | 2015-04-23 | Oxford Nanopore Technologies Limited | Modified helicases for use in nucleic acid sequencing |
| GB201406151D0 (en) | 2014-04-04 | 2014-05-21 | Oxford Nanopore Tech Ltd | Method |
| GB201406155D0 (en) | 2014-04-04 | 2014-05-21 | Oxford Nanopore Tech Ltd | Method |
| EP3097210B2 (en) | 2014-01-22 | 2022-04-20 | Oxford Nanopore Technologies Limited | Method for attaching one or more polynucleotide binding proteins to a target polynucleotide |
| GB201417712D0 (en) | 2014-10-07 | 2014-11-19 | Oxford Nanopore Tech Ltd | Method |
| GB201502810D0 (en) * | 2015-02-19 | 2015-04-08 | Oxford Nanopore Tech Ltd | Method |
| US10640822B2 (en) | 2016-02-29 | 2020-05-05 | Iridia, Inc. | Systems and methods for writing, reading, and controlling data stored in a polymer |
| GB201609221D0 (en) | 2016-05-25 | 2016-07-06 | Oxford Nanopore Tech Ltd | Method |
| WO2017203268A1 (en) | 2016-05-25 | 2017-11-30 | Oxford Nanopore Technologies Limited | Method |
| GB201609220D0 (en) | 2016-05-25 | 2016-07-06 | Oxford Nanopore Tech Ltd | Method |
| GB201616590D0 (en) | 2016-09-29 | 2016-11-16 | Oxford Nanopore Technologies Limited | Method |
| GB201620450D0 (en) | 2016-12-01 | 2017-01-18 | Oxford Nanopore Tech Ltd | Method |
| GB201707140D0 (en) | 2017-05-04 | 2017-06-21 | Oxford Nanopore Tech Ltd | Method |
| CN108101962B (zh) * | 2017-11-10 | 2021-06-04 | 浙江美保龙生物技术有限公司 | 一种特异性提高猪繁殖与呼吸综合征疫苗免疫效果的小分子多肽及其应用 |
| GB2569977A (en) | 2018-01-05 | 2019-07-10 | Oxford Nanopore Tech Ltd | Method |
| GB201801768D0 (en) | 2018-02-02 | 2018-03-21 | Oxford Nanopore Tech Ltd | Synthesis method |
| GB201808556D0 (en) | 2018-05-24 | 2018-07-11 | Oxford Nanopore Tech Ltd | Method |
| GB201808554D0 (en) | 2018-05-24 | 2018-07-11 | Oxford Nanopore Tech Ltd | Method |
| GB201809323D0 (en) | 2018-06-06 | 2018-07-25 | Oxford Nanopore Tech Ltd | Method |
| GB201821155D0 (en) | 2018-12-21 | 2019-02-06 | Oxford Nanopore Tech Ltd | Method |
| CN110004074B (zh) * | 2019-02-28 | 2021-06-18 | 北京蛋白质组研究中心 | 一种利用silac标记耻垢分枝杆菌蛋白质的方法及其专用培养基 |
| GB201907244D0 (en) | 2019-05-22 | 2019-07-03 | Oxford Nanopore Tech Ltd | Method |
| GB201907246D0 (en) | 2019-05-22 | 2019-07-03 | Oxford Nanopore Tech Ltd | Method |
| CN112079903B (zh) * | 2019-06-14 | 2022-11-18 | 中国科学院青岛生物能源与过程研究所 | 一种错配结合蛋白的突变体及其编码基因 |
| GB201917060D0 (en) | 2019-11-22 | 2020-01-08 | Oxford Nanopore Tech Ltd | Method |
| CN118011004A (zh) | 2019-12-02 | 2024-05-10 | 牛津纳米孔科技公开有限公司 | 使用纳米孔表征靶多肽的方法 |
| GB201917742D0 (en) | 2019-12-04 | 2020-01-15 | Oxford Nanopore Tech Ltd | Method |
| GB202004944D0 (en) | 2020-04-03 | 2020-05-20 | King S College London | Method |
| EP4168583A2 (en) | 2020-06-18 | 2023-04-26 | Oxford Nanopore Technologies plc | Method of characterising a polynucleotide moving through a nanopore |
| GB202009349D0 (en) | 2020-06-18 | 2020-08-05 | Oxford Nanopore Tech Ltd | Method |
| CN115698331A (zh) | 2020-06-18 | 2023-02-03 | 牛津纳米孔科技公开有限公司 | 使用检测器选择性地表征多核苷酸的方法 |
| BR112022014144A2 (pt) | 2020-06-19 | 2022-12-27 | Qitan Tech Ltd Beijing | Helicase do tipo pif1, constructo, ácido nucleico, vetor de expressão, célula hospedeira, método para controlar o movimento de um polinucleotídeo, método de caracterização de um polinucleotídeo alvo, produto para caracterizar um polinucleotídeo alvo e uso da helicase do tipo pif1 |
| CN113930406B (zh) | 2021-12-17 | 2022-04-08 | 北京齐碳科技有限公司 | 一种Pif1-like解旋酶及其应用 |
| US11837302B1 (en) | 2020-08-07 | 2023-12-05 | Iridia, Inc. | Systems and methods for writing and reading data stored in a polymer using nano-channels |
| KR102398032B1 (ko) * | 2020-09-22 | 2022-05-19 | 비피진 주식회사 | 암 치료를 위한 5''-뉴클레오티다아제 변이체 |
| GB202015993D0 (en) | 2020-10-08 | 2020-11-25 | Oxford Nanopore Tech Ltd | Method |
| CN116601297A (zh) * | 2020-12-14 | 2023-08-15 | 北京齐碳科技有限公司 | 一种经修饰的解旋酶及其应用 |
| US20240368568A1 (en) | 2021-04-06 | 2024-11-07 | Qitan Technology Ltd., Chengdu | Modified prp43 helicase and use thereof |
| CN117337333A (zh) | 2021-05-19 | 2024-01-02 | 牛津纳米孔科技公开有限公司 | 用于补体链测序的方法 |
| GB202107192D0 (en) | 2021-05-19 | 2021-06-30 | Oxford Nanopore Tech Ltd | Method |
| GB202107354D0 (en) | 2021-05-24 | 2021-07-07 | Oxford Nanopore Tech Ltd | Method |
| GB202118906D0 (en) | 2021-12-23 | 2022-02-09 | Oxford Nanopore Tech Ltd | Method |
| GB202118908D0 (en) | 2021-12-23 | 2022-02-09 | Oxford Nanopore Tech Ltd | Method |
| EP4458987A4 (en) * | 2021-12-31 | 2025-10-22 | Bgi Shenzhen | BCH1X HELICASE AND ITS USE |
| EP4482855A1 (en) * | 2022-02-22 | 2025-01-01 | Peter Biotherapeutics, Inc. | Technologies for genetic modification |
| GB202205617D0 (en) | 2022-04-14 | 2022-06-01 | Oxford Nanopore Tech Plc | Novel modified protein pores and enzymes |
| EP4519453A1 (en) | 2022-05-06 | 2025-03-12 | 10X Genomics, Inc. | Methods and compositions for in situ analysis of v(d)j sequences |
| WO2023222657A1 (en) | 2022-05-17 | 2023-11-23 | Oxford Nanopore Technologies Plc | Method and adaptors |
| CN117384867B (zh) * | 2022-09-16 | 2024-06-07 | 北京普译生物科技有限公司 | 一种经修饰的Cas3移位酶及其应用 |
| CN117721101B (zh) * | 2022-09-16 | 2024-09-20 | 北京普译生物科技有限公司 | 一种经修饰的CaPif1解旋酶及其应用 |
| GB202216162D0 (en) | 2022-10-31 | 2022-12-14 | Oxford Nanopore Tech Plc | Method |
| CN120344661A (zh) * | 2022-12-28 | 2025-07-18 | 深圳华大生命科学研究院 | 一种销结构域、含其的解旋酶及制备方法和应用 |
| WO2024138574A1 (zh) * | 2022-12-29 | 2024-07-04 | 深圳华大生命科学研究院 | 解旋酶及其应用 |
| EP4644570A1 (en) * | 2022-12-30 | 2025-11-05 | BGI Shenzhen | Helicase mutant, and preparation method therefor and use thereof in high-throughput sequencing |
| CN120435549A (zh) * | 2022-12-30 | 2025-08-05 | 深圳华大生命科学研究院 | 一种解旋酶及其制备方法和在高通量测序中的应用 |
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| GB202307486D0 (en) | 2023-05-18 | 2023-07-05 | Oxford Nanopore Tech Plc | Method |
| WO2025067293A1 (zh) * | 2023-09-27 | 2025-04-03 | 北京齐碳科技有限公司 | 解旋酶及其应用 |
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| GB202317028D0 (en) | 2023-11-06 | 2023-12-20 | Oxford Nanopore Tech Ltd | Method |
| CN118126983B (zh) * | 2024-02-22 | 2025-09-02 | 北京普译生物科技有限公司 | 一种修饰的mpk2解旋酶及其应用 |
| CN117964713B (zh) * | 2024-02-26 | 2024-07-30 | 江苏省疾病预防控制中心(江苏省公共卫生研究院) | 一种t4gp32蛋白突变体及其应用 |
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| CN119192309B (zh) * | 2024-10-25 | 2025-09-30 | 新镁(上海)生物技术有限公司 | 高耐盐单链结合蛋白突变体及其应用 |
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| CN107109380A (zh) | 2017-08-29 |
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| EP3204406A2 (en) | 2017-08-16 |
| WO2016055777A2 (en) | 2016-04-14 |
| US20240287485A1 (en) | 2024-08-29 |
| JP2017535256A (ja) | 2017-11-30 |
| GB201417712D0 (en) | 2014-11-19 |
| CN117089536A (zh) | 2023-11-21 |
| US20180179500A1 (en) | 2018-06-28 |
| KR20170063889A (ko) | 2017-06-08 |
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