CN116622671A - 经修饰的酶 - Google Patents

经修饰的酶 Download PDF

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Publication number
CN116622671A
CN116622671A CN202310481939.3A CN202310481939A CN116622671A CN 116622671 A CN116622671 A CN 116622671A CN 202310481939 A CN202310481939 A CN 202310481939A CN 116622671 A CN116622671 A CN 116622671A
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CN
China
Prior art keywords
helicase
polynucleotide
seq
amino acid
histidine
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN202310481939.3A
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English (en)
Chinese (zh)
Inventor
安德鲁·海伦
瑞贝卡·维多利亚·鲍恩
马克·布鲁斯
拉科马·贾亚辛格
约瑟夫·哈格里夫·劳埃德
绍博尔奇·苏罗尔斯
伊丽莎白·杰恩·华莱士
克里斯多夫·彼得·约德
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Oxford Nanopore Technology Public Co ltd
Original Assignee
Oxford Nanopore Technology Public Co ltd
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Filing date
Publication date
Priority claimed from PCT/GB2015/051291 external-priority patent/WO2015166276A1/en
Application filed by Oxford Nanopore Technology Public Co ltd filed Critical Oxford Nanopore Technology Public Co ltd
Publication of CN116622671A publication Critical patent/CN116622671A/zh
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y306/00—Hydrolases acting on acid anhydrides (3.6)
    • C12Y306/04—Hydrolases acting on acid anhydrides (3.6) acting on acid anhydrides; involved in cellular and subcellular movement (3.6.4)
    • C12Y306/04012—DNA helicase (3.6.4.12)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/50—Other enzymatic activities
    • C12Q2521/513—Winding/unwinding enzyme, e.g. helicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/60—Detection means characterised by use of a special device
    • C12Q2565/631—Detection means characterised by use of a special device being a biochannel or pore

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
  • Chemical Kinetics & Catalysis (AREA)
CN202310481939.3A 2014-10-07 2015-10-06 经修饰的酶 Pending CN116622671A (zh)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
GBGB1417712.5A GB201417712D0 (en) 2014-10-07 2014-10-07 Method
GB1417712.5 2014-10-07
PCT/GB2015/051291 WO2015166276A1 (en) 2014-05-02 2015-05-01 Method of improving the movement of a target polynucleotide with respect to a transmembrane pore
GBPCT/GB2015/051291 2015-05-01
CN201580066254.6A CN107109380A (zh) 2014-10-07 2015-10-06 经修饰的酶
PCT/GB2015/052916 WO2016055777A2 (en) 2014-10-07 2015-10-06 Modified enzymes

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
CN201580066254.6A Division CN107109380A (zh) 2014-10-07 2015-10-06 经修饰的酶

Publications (1)

Publication Number Publication Date
CN116622671A true CN116622671A (zh) 2023-08-22

Family

ID=51946970

Family Applications (3)

Application Number Title Priority Date Filing Date
CN201580066254.6A Pending CN107109380A (zh) 2014-10-07 2015-10-06 经修饰的酶
CN202310481939.3A Pending CN116622671A (zh) 2014-10-07 2015-10-06 经修饰的酶
CN202310481940.6A Pending CN117089536A (zh) 2014-10-07 2015-10-06 经修饰的酶

Family Applications Before (1)

Application Number Title Priority Date Filing Date
CN201580066254.6A Pending CN107109380A (zh) 2014-10-07 2015-10-06 经修饰的酶

Family Applications After (1)

Application Number Title Priority Date Filing Date
CN202310481940.6A Pending CN117089536A (zh) 2014-10-07 2015-10-06 经修饰的酶

Country Status (7)

Country Link
US (3) US11180741B2 (enExample)
EP (2) EP3204406B1 (enExample)
JP (1) JP6843042B2 (enExample)
KR (1) KR102436445B1 (enExample)
CN (3) CN107109380A (enExample)
GB (1) GB201417712D0 (enExample)
WO (1) WO2016055777A2 (enExample)

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