CN107033101A - 一种检测甲醛的荧光探针制备方法及应用 - Google Patents
一种检测甲醛的荧光探针制备方法及应用 Download PDFInfo
- Publication number
- CN107033101A CN107033101A CN201710358356.6A CN201710358356A CN107033101A CN 107033101 A CN107033101 A CN 107033101A CN 201710358356 A CN201710358356 A CN 201710358356A CN 107033101 A CN107033101 A CN 107033101A
- Authority
- CN
- China
- Prior art keywords
- formaldehyde
- preparation
- fluorescence probe
- probe
- fluorescence
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 title claims abstract description 149
- 239000000523 sample Substances 0.000 title claims abstract description 50
- 238000002360 preparation method Methods 0.000 title claims abstract description 17
- CBHOOMGKXCMKIR-UHFFFAOYSA-N azane;methanol Chemical compound N.OC CBHOOMGKXCMKIR-UHFFFAOYSA-N 0.000 claims description 12
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 claims description 9
- 235000011114 ammonium hydroxide Nutrition 0.000 claims description 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 9
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 claims description 8
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims description 8
- 229910052700 potassium Inorganic materials 0.000 claims description 8
- 239000011591 potassium Substances 0.000 claims description 8
- NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonium chloride Substances [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 claims description 7
- 239000002994 raw material Substances 0.000 claims description 7
- 238000001514 detection method Methods 0.000 claims description 6
- 239000003480 eluent Substances 0.000 claims description 3
- WSFSSNUMVMOOMR-NJFSPNSNSA-N methanone Chemical compound O=[14CH2] WSFSSNUMVMOOMR-NJFSPNSNSA-N 0.000 claims description 3
- 239000003208 petroleum Substances 0.000 claims description 3
- 238000010898 silica gel chromatography Methods 0.000 claims description 3
- 238000003756 stirring Methods 0.000 claims description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 2
- 238000004519 manufacturing process Methods 0.000 claims description 2
- 238000000746 purification Methods 0.000 claims description 2
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 claims 2
- 239000011259 mixed solution Substances 0.000 claims 2
- 229910021529 ammonia Inorganic materials 0.000 claims 1
- 238000006243 chemical reaction Methods 0.000 abstract description 6
- 238000000034 method Methods 0.000 abstract description 4
- 239000007850 fluorescent dye Substances 0.000 abstract description 3
- 239000007864 aqueous solution Substances 0.000 abstract description 2
- 239000012472 biological sample Substances 0.000 abstract description 2
- 230000015572 biosynthetic process Effects 0.000 abstract description 2
- 238000003786 synthesis reaction Methods 0.000 abstract description 2
- 150000001875 compounds Chemical class 0.000 abstract 1
- 238000005424 photoluminescence Methods 0.000 description 16
- 238000002189 fluorescence spectrum Methods 0.000 description 14
- 238000012360 testing method Methods 0.000 description 6
- 238000007792 addition Methods 0.000 description 5
- 239000012452 mother liquor Substances 0.000 description 5
- 239000000243 solution Substances 0.000 description 5
- 244000309466 calf Species 0.000 description 4
- 210000002966 serum Anatomy 0.000 description 4
- 238000001228 spectrum Methods 0.000 description 4
- WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical compound CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 description 3
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 230000005284 excitation Effects 0.000 description 2
- 125000002485 formyl group Chemical class [H]C(*)=O 0.000 description 2
- 239000007924 injection Substances 0.000 description 2
- 238000002347 injection Methods 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 0 *C1=CC(O)=*1Cc1nc(cccc2)c2[s]1 Chemical compound *C1=CC(O)=*1Cc1nc(cccc2)c2[s]1 0.000 description 1
- 238000005611 2-aza-Cope rearrangement reaction Methods 0.000 description 1
- IZGADWSEDZVYHD-UHFFFAOYSA-N CNc1ccccc1S Chemical compound CNc1ccccc1S IZGADWSEDZVYHD-UHFFFAOYSA-N 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000000711 cancerogenic effect Effects 0.000 description 1
- 231100000357 carcinogen Toxicity 0.000 description 1
- 239000003183 carcinogenic agent Substances 0.000 description 1
- 238000002485 combustion reaction Methods 0.000 description 1
- 239000012043 crude product Substances 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000004821 distillation Methods 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000007812 electrochemical assay Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 239000000446 fuel Substances 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 238000004817 gas chromatography Methods 0.000 description 1
- 238000009776 industrial production Methods 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 230000009878 intermolecular interaction Effects 0.000 description 1
- 230000007794 irritation Effects 0.000 description 1
- 238000004811 liquid chromatography Methods 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 238000000655 nuclear magnetic resonance spectrum Methods 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 238000005375 photometry Methods 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 230000007096 poisonous effect Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000000425 proton nuclear magnetic resonance spectrum Methods 0.000 description 1
- 210000002345 respiratory system Anatomy 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 150000003384 small molecules Chemical class 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 238000011895 specific detection Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D277/00—Heterocyclic compounds containing 1,3-thiazole or hydrogenated 1,3-thiazole rings
- C07D277/60—Heterocyclic compounds containing 1,3-thiazole or hydrogenated 1,3-thiazole rings condensed with carbocyclic rings or ring systems
- C07D277/62—Benzothiazoles
- C07D277/64—Benzothiazoles with only hydrocarbon or substituted hydrocarbon radicals attached in position 2
- C07D277/66—Benzothiazoles with only hydrocarbon or substituted hydrocarbon radicals attached in position 2 with aromatic rings or ring systems directly attached in position 2
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K11/00—Luminescent, e.g. electroluminescent, chemiluminescent materials
- C09K11/06—Luminescent, e.g. electroluminescent, chemiluminescent materials containing organic luminescent materials
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K2211/00—Chemical nature of organic luminescent or tenebrescent compounds
- C09K2211/10—Non-macromolecular compounds
- C09K2211/1003—Carbocyclic compounds
- C09K2211/1007—Non-condensed systems
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K2211/00—Chemical nature of organic luminescent or tenebrescent compounds
- C09K2211/10—Non-macromolecular compounds
- C09K2211/1018—Heterocyclic compounds
- C09K2211/1025—Heterocyclic compounds characterised by ligands
- C09K2211/1029—Heterocyclic compounds characterised by ligands containing one nitrogen atom as the heteroatom
- C09K2211/1037—Heterocyclic compounds characterised by ligands containing one nitrogen atom as the heteroatom with sulfur
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N2021/6417—Spectrofluorimetric devices
- G01N2021/6419—Excitation at two or more wavelengths
Abstract
本发明公开了一种检测甲醛的荧光探针制备方法及应用。属于甲醛探针的制备技术领域。该甲醛探针是一种良好的比率型荧光探针,可以用于检测水溶液和生物样品中的甲醛。本发明的甲醛荧光探针,其结构式如下:本发明同时提供了式FAP所示化合物的制备方法。该方法合成步骤简单,只需通过一步反应,产率较高。
Description
技术领域
本发明涉及一种甲醛荧光探针的制备方法及应用,属于荧光探针技术领域。
背景技术
甲醛是一种无色、有强烈刺激性气味的气体。易溶于水、醇和醚。甲醛在常温下是气态,通常以水溶液形式出现,是一种公认的致癌物质。甲醛可通过各种自然的和人为的活动产生。如燃料燃烧、工业生产、微生物的排放。甲醛是原浆毒物,能与蛋白质结合,高浓度甲醛能对人的眼睛、鼻、呼吸道和皮肤等易暴露器官产生伤害。甲醛检测方法主要有:分光光度法、电化学检测法、气相色谱法、液相色谱法、传感器法等。但每种检测方法所偏重的应用领域不同,并各有其优点和一定的局限性。因此,发明一种能快速、方便检测环境和生物样品中甲醛的荧光探针可能对与甲醛相关的疾病研究有重要的实际意义。
小分子有机荧光探针是一种将分子间的相互作用转换为光学信号传递给外界的工具。因其具有高选择性,高的检测灵敏度、并能实时在线检测等优点而备受关注。被广泛用于生物医学、环境科学等领域。它与特定目标分析物发生作用后,荧光信号会发生变化,以达到检测目的。因此,我们设计了一种基于2-aza-Cope重排反应的荧光探针,从而实现对甲醛的特异性检测。
发明内容
本发明的目的在于提供一种能检测甲醛的荧光探针,并进一步提供了探针的制备方法和应用。
一种检测甲醛的荧光探针FAP,其分子式为:C17H16N2OS;其化学结构式如下:
上述的甲醛荧光探针的合成路线如下:
上述甲醛荧光探针的制备方法,包括:
步骤一,将烯丙基三氟硼酸钾溶于氨甲醇溶液中,N2保护下,常温搅拌15min。
步骤二,将原料1溶于氨甲醇溶液中,并加入少量水,注入反应瓶中,常温反应16h,用硅胶层析柱分离纯化得到所述探针产品。
上述制备方法,烯丙基三氟硼酸钾和原料1的摩尔比为3-6:1。
上述制备方法,氨甲醇溶液的浓度为1-7mol/L。
步骤一中,烯丙基三氟硼酸钾与氨甲醇的质量比1:20-70。
步骤二中,原料1、氨甲醇、水的质量比为1-10:50-100:1。
上述制备方法,在步骤二中分离纯化所用洗脱剂体积比例为(乙酸乙酯:石油醚=1:4)。该荧光探针用于检测水环境中的甲醛。采用荧光光谱仪,激发波为350nm,随甲醛浓度的升高,荧光光谱在462nm处的荧光峰值逐渐降低,同时在541nm处产生一个新的发射带并且荧光峰值逐渐增强。
该荧光探针用于检测生物样品中的甲醛。采用荧光光谱仪,激发波为350nm,随甲醛浓度的升高,荧光光谱在462nm处的荧光峰值逐渐降低,同时在525nm处产生一个新的发射带并且荧光峰值逐渐增强。
附图说明
图1是实施例1中探针FAP的1H NMR图谱
图2是实施例1中探针FAP的1C NMR图谱
图3是实施例2探针FAP随不同当量甲醛的加入。其中,在462nm处的荧光峰值,从上至下,甲醛浓度依次为0-30mM中探针FAP的荧光光谱。在541nm处的荧光峰值,从下至上,甲醛浓度依次为0-30mM中探针FAP的荧光光谱。
图4是实施例3在小牛血清中探针FAP随不同当量甲醛的加入荧光谱图的变化情况。
其中,在462nm处的荧光峰值,从上至下,甲醛浓度依次为0-15mM中探针FAP的荧光光谱。在525nm处的荧光峰值,从下至上,甲醛浓度依次为0-15mM中探针FAP的荧光光谱。
具体实施方式
实施例1:探针FAP的合成:
将81.2mg烯丙基三氟硼酸钾溶于5mL氨甲醇溶液(浓度为7mol/L)中,氮气保护下,常温搅拌15min。
将35.1mg原料1溶于2mL氨甲醇溶液(浓度为7mol/L)中,并加入20μL水,将该反应液注入烯丙基三氟硼酸钾的氨甲醇溶液中,常温反应16h。反应完毕后,通过旋转蒸发仪减压蒸馏将溶剂除去得到粗产品。以体积比为1:4的乙酸乙酯与石油醚为洗脱剂,用200-300目硅胶层析柱进行纯化,得到黄色固体28.4mg(70%)。
实施例2探针随不同当量甲醛的加入荧光谱图的变化情况
取实施例1制备的探针FAP溶于DMSO中,制成浓度为1mM探针母液,将质量分数为37%的甲醛溶液加入蒸馏水,配制成甲醛浓度为600mM的甲醛母液。配置乙腈:PBS缓冲液(0.01mM,pH=7.4)=2:3(体积比)的光谱溶液,每根试管3mL。从探针母液中取出60μL(20μM)加入到含有3mL光谱溶液的小试管当中,加入不同浓度的甲醛(0,0.25,0.5,2,3,4,6,7,8,10,12,13,14,15,16,17,18,20,25,30mM)。用荧光光谱仪测试探针与不同当量甲醛反应液的荧光光谱变化。荧光光谱变化情况如图3所示。图3是实施例2探针FAP随不同当量甲醛的加入。其中,在462nm处的荧光峰值,从上至下,甲醛浓度范围为0-30mM中探针FAP的荧光光谱。在541nm处的荧光峰值,从下至上,甲醛浓度范围为0-30mM中探针FAP的荧光光谱。由图可见,随着加入不同当量的甲醛,探针在462nm处的荧光峰值逐渐降低,同时在541nm处产生一个新的发射带并且其荧光峰值逐渐增强。
实施例3在小牛血清中探针FAP随不同当量甲醛的荧光谱图的变化情况
取实施例2制备的探针母液和甲醛母液,配置小牛血清:PBS缓冲液(0.01mM,pH=7.4)=2:98(体积比)的光谱溶液,每根试管3mL。从探针母液中取出60μL(20μM)加入到含有3mL光谱溶液的小试管当中,加入不同浓度的甲醛(0,0.25,0.5,0.75,1,1.5,2,3,3.5,4,4.5,5,6,7,8,9,10,12,15mM)。用荧光光谱仪测试探针与不同当量甲醛反应液的荧光光谱变化。荧光光谱变化情况如图4所示。图4是实施例3在小牛血清中探针FAP随不同当量甲醛的加入荧光谱图的变化情况。其中,在462nm处的荧光峰值,从上至下,甲醛浓度范围为0-15mM中探针FAP的荧光光谱。在525nm处的荧光峰值,从下至上,甲醛浓度范围为0-15mM中探针FAP的荧光光谱。由图可见,随着加入不同当量的甲醛,探针在461nm处的荧光峰值逐渐降低,同时在525nm处产生一个新的发射带并且其荧光峰值逐渐增强。
Claims (9)
1.一种检测甲醛的荧光探针,其特征在于:该荧光探针的分子式为:C17H16N2OS;其化学结构式如下:
2.根据权利要求1所述的检测甲醛的荧光探针的制备方法,合成路线如下:
具体制备方法包括如下:
步骤一,将烯丙基三氟硼酸钾溶于氨甲醇溶液中,N2保护下,常温搅拌15min;
步骤二,将原料1溶于氨甲醇溶液中,并加入水,将混合溶液加入到步骤一的混合溶液中,常温下反应16h,用硅胶层析柱分离纯化得到权利要求1所述的探针产品。
3.根据权利要求1所述的制备方法,其特征在于:烯丙基三氟硼酸钾和原料1的摩尔比为3-6:1。
4.根据权利要求1所述的制备方法,其特征在于:氨甲醇溶液的浓度为1-7mol/L。
5.根据权利要求1所述的制备方法,其特征在于:步骤一中,烯丙基三氟硼酸钾与氨甲醇的质量比1:20-70。
6.根据权利要求1所述的制备方法,其特征在于:步骤二中,原料1、氨甲醇、水的质量比为1-10:50-100:1。
7.根据权利要求1所述的制备方法,其特征在于:在步骤二中分离纯化所用洗脱剂体积比例为:乙酸乙酯:石油醚=1:4。
8.根据权利要求1所述的检测甲醛的荧光探针,其特征在于:该荧光探针用于检测水环境中的甲醛。
9.根据权利要求1所述的检测甲醛的荧光探针,其特征在于:该荧光探针用于检测生物样品中的甲醛。
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201710358356.6A CN107033101B (zh) | 2017-05-19 | 2017-05-19 | 一种检测甲醛的荧光探针制备方法及应用 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201710358356.6A CN107033101B (zh) | 2017-05-19 | 2017-05-19 | 一种检测甲醛的荧光探针制备方法及应用 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN107033101A true CN107033101A (zh) | 2017-08-11 |
CN107033101B CN107033101B (zh) | 2019-04-02 |
Family
ID=59538816
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201710358356.6A Active CN107033101B (zh) | 2017-05-19 | 2017-05-19 | 一种检测甲醛的荧光探针制备方法及应用 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN107033101B (zh) |
Cited By (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN108148014A (zh) * | 2018-01-03 | 2018-06-12 | 济南大学 | 一种甲醛荧光探针及其制备方法和应用 |
CN108219776A (zh) * | 2018-02-05 | 2018-06-29 | 三峡大学 | 一种增强型荧光探针、制备方法及在检测甲醛上的应用 |
CN108444962A (zh) * | 2018-03-05 | 2018-08-24 | 北京化工大学 | 一种基于苝的甲醛比色探针和甲醛荧光试纸、其制备方法与使用方法 |
CN109574971A (zh) * | 2019-01-03 | 2019-04-05 | 济南大学 | 一种双光子甲醛荧光探针及其制备方法和应用 |
CN110117229A (zh) * | 2018-02-05 | 2019-08-13 | 中国医学科学院药物研究所 | 一类荧光探针及其制备方法与应用 |
CN111087362A (zh) * | 2019-12-18 | 2020-05-01 | 商丘师范学院 | 一种高选择性检测甲醛的荧光探针及其合成方法与应用 |
CN112521383A (zh) * | 2020-12-11 | 2021-03-19 | 三峡大学 | 苯并噻唑衍生物及作为荧光染料上的应用 |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104262287A (zh) * | 2014-09-02 | 2015-01-07 | 苏州罗兰生物科技有限公司 | 一种亚硫酸根比率荧光探针的制备及应用 |
CN105372217A (zh) * | 2015-11-17 | 2016-03-02 | 济南大学 | 一种甲醛荧光探针及其制备方法、应用 |
CN105924394A (zh) * | 2016-05-20 | 2016-09-07 | 浙江工业大学 | 一种双光子甲醛荧光探针及其制备与应用 |
WO2017034927A1 (en) * | 2015-08-21 | 2017-03-02 | The Regents Of The University Of California | Homoallylamines as formaldehyde-responsive triggers |
-
2017
- 2017-05-19 CN CN201710358356.6A patent/CN107033101B/zh active Active
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104262287A (zh) * | 2014-09-02 | 2015-01-07 | 苏州罗兰生物科技有限公司 | 一种亚硫酸根比率荧光探针的制备及应用 |
WO2017034927A1 (en) * | 2015-08-21 | 2017-03-02 | The Regents Of The University Of California | Homoallylamines as formaldehyde-responsive triggers |
CN105372217A (zh) * | 2015-11-17 | 2016-03-02 | 济南大学 | 一种甲醛荧光探针及其制备方法、应用 |
CN105924394A (zh) * | 2016-05-20 | 2016-09-07 | 浙江工业大学 | 一种双光子甲醛荧光探针及其制备与应用 |
Non-Patent Citations (4)
Title |
---|
JUNCHAO XU等: ""A simplenaphthalene-based fluorescentprobeforhighselective detectionofformaldehydeintoffeesandHeLacells via aza-Cope reaction"", 《TALANTA》 * |
THOMAS F. BREWER: ""An Aza-Cope Reactivity-Based Fluorescent Probe for Imaging Formaldehyde in Living Cells"", 《J. AM. CHEM. SOC.》 * |
XINGJIANG LIU等: ""A ratiometric fluorescent probe for rapid, sensitive and selective detection of sulfur dioxide with large Stokes shifts by single wavelength excitation"", 《ORG. BIOMOL. CHEM.》 * |
ZHENDA XIE等: ""A highly selective two-photon fluorogenic probe for formaldehydeand and its bioimaging application in cells and zebrafish"", 《SENSORS AND ACTUATORS B: CHEMICAL》 * |
Cited By (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN108148014A (zh) * | 2018-01-03 | 2018-06-12 | 济南大学 | 一种甲醛荧光探针及其制备方法和应用 |
CN108219776A (zh) * | 2018-02-05 | 2018-06-29 | 三峡大学 | 一种增强型荧光探针、制备方法及在检测甲醛上的应用 |
CN110117229A (zh) * | 2018-02-05 | 2019-08-13 | 中国医学科学院药物研究所 | 一类荧光探针及其制备方法与应用 |
CN108219776B (zh) * | 2018-02-05 | 2020-09-22 | 三峡大学 | 一种增强型荧光探针、制备方法及在检测甲醛上的应用 |
CN110117229B (zh) * | 2018-02-05 | 2021-12-07 | 中国医学科学院药物研究所 | 一类荧光探针及其制备方法与应用 |
CN108444962A (zh) * | 2018-03-05 | 2018-08-24 | 北京化工大学 | 一种基于苝的甲醛比色探针和甲醛荧光试纸、其制备方法与使用方法 |
CN108444962B (zh) * | 2018-03-05 | 2021-01-12 | 北京化工大学 | 一种基于苝的甲醛比色探针和甲醛荧光试纸、其制备方法与使用方法 |
CN109574971A (zh) * | 2019-01-03 | 2019-04-05 | 济南大学 | 一种双光子甲醛荧光探针及其制备方法和应用 |
CN111087362A (zh) * | 2019-12-18 | 2020-05-01 | 商丘师范学院 | 一种高选择性检测甲醛的荧光探针及其合成方法与应用 |
CN111087362B (zh) * | 2019-12-18 | 2022-10-11 | 商丘师范学院 | 一种高选择性检测甲醛的荧光探针及其合成方法与应用 |
CN112521383A (zh) * | 2020-12-11 | 2021-03-19 | 三峡大学 | 苯并噻唑衍生物及作为荧光染料上的应用 |
Also Published As
Publication number | Publication date |
---|---|
CN107033101B (zh) | 2019-04-02 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN107033101B (zh) | 一种检测甲醛的荧光探针制备方法及应用 | |
CN105372217A (zh) | 一种甲醛荧光探针及其制备方法、应用 | |
CN105131935B (zh) | 快速高选择性氟离子比色探针及其制备方法 | |
CN103589423B (zh) | 一种含n,n-二乙基对甲氨基酚的荧光探针及其在检测苯硫酚中的应用 | |
CN108047060A (zh) | 一种识别检测甲醛的芘衍生物荧光探针分子及其制备方法和应用 | |
CN112142639B (zh) | 一种基于醛基的手性氨基酸识别探针及其制备方法和应用 | |
CN107141256A (zh) | 一种快速高选择性超灵敏硫化氢比率荧光探针及其制备方法 | |
Wang et al. | A diboronic acid fluorescent sensor for selective recognition of d-ribose via fluorescence quenching | |
CN114773288A (zh) | 一种区别检测半胱氨酸和同型半胱氨酸/谷胱甘肽的荧光探针及其制备方法和应用 | |
CN112724166B (zh) | 一种水溶性荧光探针及其合成方法和用于检测抗生素的用途 | |
CN110092773A (zh) | 一种氧杂蒽类衍生物及其制备方法和应用 | |
CN111233928B (zh) | 一种香豆素衍生物Mito-Cys及其制备方法和应用 | |
CN108774226A (zh) | 一种用于检测银离子的荧光探针及其制备方法与应用 | |
CN109734710A (zh) | 一种检测半胱氨酸的荧光探针及其合成方法与应用 | |
CN115047093B (zh) | 一种无水咖啡因中硫酸二甲酯检测方法 | |
CN110894193B (zh) | 一种新型荧光探针的合成及在检测半胱氨酸中的应用 | |
CN104502473B (zh) | 一种利用hplc法检测单氰胺的定量方法 | |
CN113214184B (zh) | 一种用于检测甲醛的荧光探针及其制备方法和应用 | |
CN105418560B (zh) | 长波长肼比色荧光探针及其制备方法 | |
Wang et al. | Design and construction of novel assemblies for monosaccharides and glycosides detection | |
CN105572063A (zh) | 一种基于氯化血红素可控聚集的水胺硫磷便捷检测方法 | |
CN107917903A (zh) | 糖基低维材料在甲型流感病毒荧光检测中的应用 | |
CN108760697B (zh) | 一种氟硼二吡咯衍生物bdp-n3及其合成方法和用途 | |
CN111087362A (zh) | 一种高选择性检测甲醛的荧光探针及其合成方法与应用 | |
CN109632736A (zh) | 一种检测马兜铃酸a荧光传感器的制备方法 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
GR01 | Patent grant | ||
GR01 | Patent grant | ||
TR01 | Transfer of patent right |
Effective date of registration: 20240321 Address after: 443000 No.55, development avenue, Yichang District, China (Hubei) free trade zone, Yichang City, Hubei Province Patentee after: YICHANG HENGKANG TECHNOLOGY Co.,Ltd. Country or region after: Zhong Guo Address before: 443002 No. 8, University Road, Yichang, Hubei Patentee before: CHINA THREE GORGES University Country or region before: Zhong Guo |
|
TR01 | Transfer of patent right |