CN106995327A - The formula and preparation method of a kind of edible mushroom main pipe culture medium - Google Patents
The formula and preparation method of a kind of edible mushroom main pipe culture medium Download PDFInfo
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- CN106995327A CN106995327A CN201610043962.4A CN201610043962A CN106995327A CN 106995327 A CN106995327 A CN 106995327A CN 201610043962 A CN201610043962 A CN 201610043962A CN 106995327 A CN106995327 A CN 106995327A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F5/00—Fertilisers from distillery wastes, molasses, vinasses, sugar plant or similar wastes or residues, e.g. from waste originating from industrial processing of raw material of agricultural origin or derived products thereof
- C05F5/002—Solid waste from mechanical processing of material, e.g. seed coats, olive pits, almond shells, fruit residue, rice hulls
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Abstract
The invention discloses a kind of formula of edible mushroom main pipe culture medium and preparation method, it completes its preparation method by mung bean sprouts, wheat bran, agar, white sugar, six kinds of raw materials of active small molecular and pure water by seven technological processes of A, B, C, D, E, F, G；This edible mushroom main pipe culture medium can realize the parent species high nutrition culture of edible mushroom, culture base-material formula is reasonable, physical and chemical index is reliable, comply fully with the growth requirement of edible mushroom parent species, it is of high nutritive value than conventional PDA culture medium, diseases and insect pests resistance is strong, and cost is low, biological efficiency is high, improves the transformation efficiency of edible fungus cultivation matrix, the greatest requirements of edible mushroom stable and high yields are ensure that, the economic benefit of manufacturing enterprise is also directly enhanced；The breed of edible fungus matrix bacterium germination provided is fast, can substantially reduce bacterial contamination rate.
Description
Technical field：
The present invention relates to a kind of formula of edible mushroom main pipe culture medium and preparation method, belong to fungus growing technique field.
Background technology：
Edible mushroom as 21st century rising industry, with its high protein, several amino acids, multivitamin, low fat, no dirt
Dye, non-harmful feature, as one of domestic and international consumer's Main Foods, greatly enriches the material life of people, near several
At home and abroad in the market sales volume is significantly increased for year edible mushroom, and traditional production technology can not meet growing demand；
Edible mushroom can be divided into Wood rot type and the rotten type of grass according to its habit, and Wood rot type edible mushroom is, using wood materials as primary raw material, to divide
Solve the stronger class edible mushroom of lignin ability, such as mushroom, pick up the ears, black fungus and asparagus, the cultivation of Wood rot type edible mushroom
Mode is divided into segment wood cultivated and substituting stuff cultivation, and grass corruption type edible mushroom is, using straw kind material as main culturing raw material, to decompose fiber
The stronger class edible mushroom of plain ability, such as agaricus bisporus, straw mushroom, coprinus comatus and dictyophora phalloidea；So-called " generation material ", refers to replace Duan Mu
The various organic matters of Wood rot type edible mushroom are cultivated, substituting stuff cultivation edible mushroom can not only protect forest, and with the production cycle
Short, biological efficiency is high, the advantages of be easy to factorial praluction, and biological efficiency refers to that edible mushroom fresh weight is done with compost used
The ratio between weight, conventional percentage is represented, the dry compost production 80kg fresh food bacterium of such as 100kg, then the biology of this edible mushroom
Efficiency is 80%, and biological efficiency is also referred to as conversion ratio, is cultivated using the byproduct of the straw, branch and brewery industry of agriculture and forestry
Edible mushroom, can also eliminate environmental pollution, and its accessory substance can also produce organic fertilizer, promote the development of organic agriculture.
The content of the invention：
It is an object of the invention to provide a kind of formula of edible mushroom main pipe culture medium and preparation method.
The technical problems to be solved by the invention are achieved through the following technical solutions：A kind of edible mushroom main pipe cultivates base
Matter, it by mung bean sprouts 1, wheat bran 2, agar 3, white sugar 4, active small molecular 5 and 6 six kinds of raw materials of pure water by A, B, C, D, E, F,
Seven technological processes of G complete its preparation method, and the mass ratio of its raw material is：The ︰ of 3 ︰ white sugar of mung bean sprouts 1 ︰ wheat brans, 2 ︰ agar 4 activity
The ︰ 980~1050 of 18~25 ︰ of small molecule 5 ︰ pure water, 6=240~260 ︰, 95~105 ︰, 20~25 ︰ 1~1.5；
Preferably, described breed of edible fungus matrix is made up of the supplementary material of following percentage by weight：The ︰ fine jades of 1 ︰ wheat brans of mung bean sprouts 2
The ︰ 1000 of 20 ︰ of fat 3 ︰ white sugar, 4 ︰ active small moleculars, 5 ︰ pure water, 6=250 ︰, 100 ︰, 22 ︰ 1.25；
Described agar 3 is here as coagulator；
Described active small molecular 5 is primarily referred to as the solvent containing microelements of calcium and magnesium and compound amino acid, described compound ammonia
Base acid refers to broad bean powder（Western powder）Be made powdered of re-dry or with yellow pulvis after tail water is refined；
The preparation method of edible mushroom main pipe culture medium is as follows：
A：It is more standby than mung bean sprouts 1, wheat bran 2, agar 3, white sugar 4, active small molecular 5 and pure water 6 by above-mentioned quality, wherein mung bean
Bud 1 need to grow fine and no disease and pests harm；Wheat bran 2 is dried and no disease and pests harm；It is high, elastic from soma, white bright, the clean transparency of color
Greatly, the strong high-quality agar 3 of tough and tensile, fastness；
B：Pure water 6 is put into boiled in glassware；
C：Reach that 100 DEG C of boiling points are counted from pure water 6, addition mung bean sprouts 1, which boils mung bean sprouts 1 to be pulled out after 7 minutes, to be drained, and is retained
Mixed liquor；
D：Wheat bran 2 is added in above-mentioned mixed liquor and reaches that 100 DEG C of boiling points are counted to boil after 10 minutes and takes juice with filtered through gauze；
E：Agar 3 and white sugar 4 are added in the juice that D steps are obtained, somewhat boiling has viscosity；
F：Active small molecular 5 is added when the mucus temperature obtained in E steps is down to 40 DEG C or so；
G：Finally pour into test tube sealing preserve stand-by, every 10 milliliters of test tube；
Aforesaid operations are carried out in gnotobasis.
As a result of above technical scheme, its goal of the invention, this edible mushroom main pipe culture medium energy are preferably realized
The parent species high nutrition culture of edible mushroom is realized, culture base-material formula is reasonable, physical and chemical index is reliable, complies fully with parent edible fungus
Plant growth to require, be of high nutritive value than conventional PDA culture medium, diseases and insect pests resistance is strong, and cost is low, biological efficiency is high, carries
The high transformation efficiency of edible fungus cultivation matrix, it is ensured that the greatest requirements of edible mushroom stable and high yields, also directly enhances production
The economic benefit of enterprise；The breed of edible fungus matrix bacterium germination provided is fast, can substantially reduce bacterial contamination rate.
Brief description of the drawings：
Accompanying drawing 1 is present invention process flow chart.
Accompanying drawing 2 is test result subordinate list in the embodiment of the present invention 2（One）.
Accompanying drawing 3 is test result subordinate list in the embodiment of the present invention 2（Two）.
Accompanying drawing 4 is test result subordinate list in the embodiment of the present invention 2（Three）.
Description of reference numerals is shown in specification last page form.
Embodiment：
The content of the invention is described further with reference to embodiment：
Embodiment 1：
From the content of the invention, a kind of edible mushroom main pipe culture medium, it is by mung bean sprouts 1, wheat bran 2, agar 3, white sugar 4, activity
Small molecule 5 completes its preparation method, its raw material with 6 six kinds of raw materials of pure water by seven technological processes of A, B, C, D, E, F, G
Mass ratio be：The ︰ 20 of 6=240~260 ︰ of mung bean sprouts 1 ︰ wheat brans, 2 ︰ agar, 3 ︰ white sugar, 4 ︰ active small moleculars, 5 ︰ pure water 95~105
The ︰ 980~1050 of 18~25 ︰ of~25 ︰ 1~1.5；
Preferably, described breed of edible fungus matrix is made up of the supplementary material of following percentage by weight：The ︰ fine jades of 1 ︰ wheat brans of mung bean sprouts 2
The ︰ 1000 of 20 ︰ of fat 3 ︰ white sugar, 4 ︰ active small moleculars, 5 ︰ pure water, 6=250 ︰, 100 ︰, 22 ︰ 1.25；
Described agar 3 is here as coagulator；
Described active small molecular 5 is primarily referred to as the solvent containing microelements of calcium and magnesium and compound amino acid, described compound ammonia
Base acid refers to broad bean powder（Western powder）Be made powdered of re-dry or with yellow pulvis after tail water is refined；
The preparation method of edible mushroom main pipe culture medium is as follows：
A：It is more standby than mung bean sprouts 1, wheat bran 2, agar 3, white sugar 4, active small molecular 5 and pure water 6 by above-mentioned quality, wherein mung bean
Bud 1 need to grow fine and no disease and pests harm；Wheat bran 2 is dried and no disease and pests harm；It is high, elastic from soma, white bright, the clean transparency of color
Greatly, the strong high-quality agar 3 of tough and tensile, fastness；
B：Pure water 6 is put into boiled in glassware；
C：Reach that 100 DEG C of boiling points are counted from pure water 6, addition mung bean sprouts 1, which boils mung bean sprouts 1 to be pulled out after 7 minutes, to be drained, and is retained
Mixed liquor；
D：Wheat bran 2 is added in above-mentioned mixed liquor and reaches that 100 DEG C of boiling points are counted to boil after 10 minutes and takes juice with filtered through gauze；
E：Agar 3 and white sugar 4 are added in the juice that D steps are obtained, somewhat boiling has viscosity；
F：Active small molecular 5 is added when the mucus temperature obtained in E steps is down to 40 DEG C or so；
G：Finally pour into test tube sealing preserve stand-by, every 10 milliliters of test tube；
Aforesaid operations are carried out in gnotobasis.
Mung bean sprouts 1 of the present invention refers to：Fresh mung bean sprouts without any pest and disease damage；For the seed of legume mung bean
The tender shoots sent after soaking；Mung bean is in germination process, and vitamin C can increase a lot, and partially protein can also be decomposed
For the amino acid needed for various people, seven times of the former content of mung bean are can reach, so the nutritive value of mung bean sprouts is bigger than mung bean.
Wheat bran 2 of the present invention refers to：For the outermost epidermis of wheat；Containing a large amount of nutritional ingredients needed by human,
Its p-aminobenzoic acid content is highest in plant, and p-aminobenzoic acid is human body cell division required material, and has recovery
Coat color is acted on；Contain quite abundant vitamin E and B family vitamin.
Agar 3 of the present invention refers to：For solid colourless, without solid shape, hot water is dissolved in, in the food industry should
With extensive, bacteria culture media is also commonly used for；With coagulability, stability can form the physical chemistry such as complex compound with some materials
Property, can be used as thickener, coagulator, suspending agent, emulsifying agent, antistaling agent and stabilizer；Herein as coagulator.
White sugar 4 of the present invention refers to：The refined sugar that the molasses squeezed out by sugarcane and beet are made；Sucrose is Starch synthesis
Precursor substance in Plant Tissue Breeding sucrose be one of optimal carbon source, its function as carbon source and maintain permeate
Pressure；Gene expression and part storage protein accumulation of the sucrose to some important enzymes all have material impact.
Active small molecular 5 of the present invention refers to：Solvent containing microelements of calcium and magnesium and compound amino acid, it is described
Compound amino acid refers to broad bean powder（Western powder）Be made powdered of re-dry or with yellow pulvis after tail water is refined.
Pure water 6 of the present invention refers to：The water free from foreign meter for eliminating bacterium.
Embodiment 2：
According to the speed of growth of inclined-plane mycelia, dense degree can primarily determine that facilitation of the active material to edible mushroom, so as to
To determine main active substances --- microelements of calcium and magnesium and the proportional quantity of compound amino acid in active small molecular：
Experiment is from high temperature flat mushroom " Hunan heat 1 ", " Fu Yuan " and wide warm flat mushroom, " white flat No. 1 ", " white flat No. 2 ", and culture medium is selected
The culture medium of edible mushroom main pipe culture medium of the present invention is added, two steps of experiment point are completed：The first step tests single-activity
Effect of the material to mycelia, draws the Sq of single-activity material；Second step tests the different Sqs of two kinds of active materials
The effect to mycelia is combined, so that it is determined that the best proportional quantity of a cost performance.
（1）Testing for traces element calcium and magnesium：
We have first used 9/1000ths microelements of calcium and magnesium from thousand point one, and it is X0, X1 ... X5 ... X9 totally 10 that they, which are numbered,
Group, every group of 5 repetitions, point 2 kinds --- " Hunan heat 1 ", " Fu Yuan " is compareed；First day temperature is 11 DEG C -12 DEG C, due to
Temperature is too low not to be sprouted, in the heating of the 5th day evening, the 6th day morning 9：00 temperature reaches 26 DEG C, observation have X0, X1, X2, X3,
X4 starts to sprout；Whole culture terminates for 12 days, during which 32 DEG C of maximum temperature, 20 DEG C of minimum temperature, and X9 does not sprout in culture,
X7, X8 sprout very slow, and the mycelia later stage is obstructed not material feeding；It can be seen that active small molecular concentration is in one thousandth after the completion of test
It is suitable to 3/1000ths, find that X5, X6, X7 mycelia have physiological ageing phenomenon under observation, test result is seen attached list（One）；
（2）Test compound amino acid：
Compound amino acid of the one thousandth to 9/1000ths has first been used in test；They are numbered：Totally 10 groups of A0 ... A1 ... A5 ... A9,
Every group of 5 repetitions；Point 2 kinds --- " Hunan heat 1 ", " Fu Yuan " is compareed, and to the 3rd day temperature is 11 after this test inoculation
DEG C -12 DEG C thus do not sprout, the 3rd day evening started to warm up, and early temperature is 26 DEG C within the 4th day, and observation only has A6 not sprout, A1,
A2, A3, A8, A9 sprout very fast, from the point of view of the dense degree of mycelia, all dense not as good as microelements of calcium and magnesium, from speed of germinating
The speed of germinating for being faster than microelements of calcium and magnesium is seen, was terminated by the tenth day it can be seen that the dense degree of compound amino acid is at thousand points
One of to 4/1000ths conveniently, test result is seen attached list（Two）；
（3）Test active small molecular and effect of the combination of amino acids to mycelia：
It is contemplated that to the interaction of two kinds of active materials in test, therefore it have selected respectively from one thousandth to 5/1000ths
The combination of two kinds of active materials, has 25 combinations, i.e. A1X1 ... A1X5 ... A2X5 ... A3X5 ... A5X5 is per 2 product of component
Kind --- " white flat No. 1 ", " white flat No. 2 ", 5 repetitions；Length of testing speech nine days, test is heated up for first day with 200W bulbs, temperature
Control is between 17 DEG C -28 DEG C；Second day observation " white flat No. 2 " have A5X1, A4X1, A5X3, A5X5, A3X3, A1X3, A3X2,
A5X2, A4X4 start to sprout, and " white flat No. 1 " is not sprouted, and " white flat No. 1 " starts to sprout within the 4th day, but ARX1, ARX5 do not sprout
Hair, the 8th day observation " white flat No. 2 " ARX1 sprouts that fast but mycelia is sparse, and ARX5, which sprouts, to be suppressed, ARX2, ARX3 sprout compared with
It hurry up, mycelia is dense, " white flat No. 1 " and " white flat No. 2 " almost, but overall speed of germinating and the dense degree of mycelia are all not as good as " in vain flat 2
Number ", test result is seen attached list（Three）；
Preliminary examination terminates to combine three subordinate lists and the broad-spectrum of economic cost performance and strain draws compound amino acid 3/1000ths and micro-
The optimum proportioning of secondary element calcium and magnesium 2/1000ths.
A refers to compound amino acid
X refers to microelements of calcium and magnesium
R refers to dense degree permillage
It is sparse+, it is sparse ++, it is richer +++, it is dense ++++, it is special dense +++ ++
It is very slow+, it is slow ++, it is very fast +++, it is fast ++++, it is express +++ ++
| Reference numerals | Mark title | Reference numerals | Mark title |
| 1 | Mung bean sprouts 1 | 4 | White sugar 4 |
| 2 | Wheat bran 2 | 5 | Active small molecular 5 |
| 3 | Agar 3 | 6 | Pure water 6 |
Claims (4)
1. a kind of edible mushroom main pipe culture medium, it is characterized in that it is by mung bean sprouts (1), wheat bran (2), agar (3), white sugar (4), work
Property small molecule (5) constituted with (6) six kinds of raw materials of pure water；The mass ratio of its raw material is：Mung bean sprouts (1) ︰ wheat brans (2) ︰ agar
(3) ︰ white sugar (the 4) ︰ active small moleculars (︰ 980 of 5 18~25 ︰ of) ︰ pure water (6)=240~260 ︰ 95~105 ︰, 20~25 ︰ 1~1.5
~1050；
Preferably, described breed of edible fungus matrix is made up of the supplementary material of following percentage by weight：Mung bean sprouts (1) ︰ wheat brans
(2) ︰ agar (3) ︰ white sugar (the 4) ︰ active small moleculars (︰ 1000 of 5 20 ︰ of) ︰ pure water (6)=250 ︰ 100 ︰, 22 ︰ 1.25.
2. a kind of edible mushroom main pipe culture medium according to claim 1, it is characterized in that described agar (3) is made here
For coagulator.
3. a kind of edible mushroom main pipe culture medium according to claim 1, it is characterized in that described active small molecular (5) is main
Refer to the solvent containing microelements of calcium and magnesium and compound amino acid, described compound amino acid refers to broad bean powder（Western powder）Tail water
Be made powdered of re-dry or with yellow pulvis after refinement；The wherein He of compound amino acid 3/1000ths in active small molecular (5)
Trace elements iron, calcium and magnesium 2/1000ths are optimum proportioning amount.
4. a kind of preparation method of edible mushroom main pipe culture medium, it is characterized in that it passes through seven technique streams of A, B, C, D, E, F, G
Journey completes its preparation method；
A：By above-mentioned quality than mung bean sprouts (1), wheat bran (2), agar (3), white sugar (4), active small molecular (5) and pure water (6)
Standby, wherein mung bean sprouts (1) need to grow fine and no disease and pests harm；Wheat bran (2) is dried and no disease and pests harm；From soma, color it is white it is bright,
The strong high-quality agar (3) of high, elastic big, the tough and tensile, fastness of clean transparency；
B：Pure water (6) is put into boiled in glassware；
C：Reach that 100 DEG C of boiling points are counted from pure water (6), add mung bean sprouts (1) and boil after (7) minute and pull mung bean sprouts (1) out
Drain, retain mixed liquor；
D：Wheat bran (2) is added in above-mentioned mixed liquor and reaches that 100 DEG C of boiling points are counted to boil after 10 minutes and takes juice with filtered through gauze；
E：Agar (3) and white sugar (4) are added in the juice that D steps are obtained, somewhat boiling has viscosity；
F：Active small molecular (5) is added when the mucus temperature obtained in E steps is down to 40 DEG C or so；
G：Finally pour into test tube sealing preserve stand-by, every 10 milliliters of test tube；
Aforesaid operations are carried out in gnotobasis.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112042471A (en) * | 2020-09-21 | 2020-12-08 | 上海市农业科学院 | Industrialized production method of clitocybe maxima |
| CN114766283A (en) * | 2022-04-27 | 2022-07-22 | 贵州大学 | Auricularia polytricha culture medium containing walnut sawdust and preparation method thereof |
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| CN104871821A (en) * | 2015-06-01 | 2015-09-02 | 四川省农业科学院土壤肥料研究所 | Phellinus igniarius strain and culturing method for same |
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- 2016-01-23 CN CN201610043962.4A patent/CN106995327A/en active Pending
Patent Citations (1)
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| CN104871821A (en) * | 2015-06-01 | 2015-09-02 | 四川省农业科学院土壤肥料研究所 | Phellinus igniarius strain and culturing method for same |
Non-Patent Citations (2)
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| 孙秀梅 王福海 主编: "《农业生物技术》", 31 January 2006, 中国农业出版社 * |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112042471A (en) * | 2020-09-21 | 2020-12-08 | 上海市农业科学院 | Industrialized production method of clitocybe maxima |
| CN114766283A (en) * | 2022-04-27 | 2022-07-22 | 贵州大学 | Auricularia polytricha culture medium containing walnut sawdust and preparation method thereof |
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Application publication date: 20170801 |