CN106957837A - A kind of method that fixing lactic acid bacteria prepares cigarette Semen Persicae extract - Google Patents
A kind of method that fixing lactic acid bacteria prepares cigarette Semen Persicae extract Download PDFInfo
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Abstract
The present invention provides a kind of method that fixing lactic acid bacteria prepares cigarette Semen Persicae extract.The preparation method is concretely comprised the following steps：(1) peach kernel water extraction at 95~100 DEG C is taken repeatedly, to merge extract solution, obtain Semen Persicae extract A；(2) using lactobacillus inoculum cultivate into broth bouillon centrifugation after obtain wet thallus, in the mixed solution that wet thallus is added to polyvinyl alcohol and sodium alginate, and with contain 1~3%CaCl2Boric acid saturated solution and saturation Na2SO4PVA SA fixing lactic acid bacteria beads are crosslinked to obtain in solution；(3) by Semen Persicae extract A and fixing lactic acid bacteria bead according to mass ratio 5~10:1 mixing after fermentation, then filters to obtain clarified broth, adds alcoholic solution precipitation, filters to take supernatant concentration and obtain cigarette Semen Persicae extract.The present invention has abundant cigarette perfume, increases fragrant and sweet rhythm and the fragrant note of acid, and increase flue gas promotes the production of body fluid sense, and soft fine and smooth flue gas reduces excitant, improvement throat comfortableness.
Description
Technical field
The present invention relates to cigarette additive field, and in particular to prepares cigarette Semen Persicae extract using fixing lactic acid bacteria
Preparation method.
Background technology
Flavoring and casing is the key link in cigarette process, is the determinant and raising cigarette flavor for improving product style
One of effective means of quality.Current essence spice for cigarette is main or passes through the extraction to natural animal-plant, chemical synthesis system
It is standby.Due to by natural plant resource is limited and the harsher limitation of chemical synthesis condition.With the development of biotechnology, in recent years
Come, prepare cigarette flavor using microbe fermentation method, an economy, efficient approach are provided for the production of cigarette flavor,
Develop the new cigarette flavor that some conventional methods can not be produced.
Immobilized microorganism technology, is to be fixed on special microorganism on the carrier of choosing card, makes its highly dense and protect
Bioactivity is held, the biotechnology that can quickly, largely breed under condition of compatibility can make up suspension microorganism fermentation process
The shortcomings of efficiency is low, separation of solid and liquid is difficult, mithridatism is poor.Therefore, in recent years, the research of immobilized microorganism technology is lived very much
Jump, development is very fast, has application to many industrial departments such as food fermentation industry, environmental protection, new energy development and biological biography
Sensor etc., and deep development is achieved, show powerful superiority.It is mainly concerned with the following aspects：In food hair
It is used to produce ethanol, beer, Yoghourt etc. in ferment industry；It is used for environmental monitoring and the processing of industrial wastewater in environmental protection；
It is used to produce hydrogen, methane, biochemical fuel cell, microorganism battery etc. in new energy development；Immobilized microorganism is alternatively arranged as molecule
Recognition component, for making various biology sensors.And it is continuous ripe, perfect with fixation of microbe technology, gradually to height
The direction such as effect low energy consumption, low cost, simplification, safety automation, pollution-free is developed, dirty in energy crisis, shortage of resources, environment
In the case that dye is on the rise, it thus will be promoted more deeply and to be widely applied in terms of wastewater treatment, with more deepening
Remote meaning.Especially in terms of wastewater treatment, have great application prospect.But preparing cigarette using immobilized microorganism technology
Report is but had no with Semen Persicae extract.
Peach kernel, Chinese medicine name is rosaceous plant peach (scientific name：Prunus persica (L.) Batsch) or mountain peach (
Name：Prunus davidiana (Carr.) Franch.) dry mature seed.Harvest, remove after peach or mountain peach fruit maturation
Pulp and nucleocapsid are removed, seed is taken out, dries.Peach kernel has promoting blood circulation, relaxes bowel, relieving cough and asthma effect.For treating
Closed dysmenorrhea, lump in the abdomen lump in the abdomen lump in the abdomen, lung carbuncle acute appendicitis, injury from falling down, dry constipation of intestines, the illness such as cough and asthma.Using peach kernel as the peach of raw material
Benevolence medicinal extract, Semen Persicae extract are widely used in cigarette, with effects such as abundant cigarette perfume, increase semen armeniacae amarae notes.
Lactic acid bacteria (lactic acid bacteria, LAB) is that a class can be produced largely using fermentable carbohydrate
The common name of the bacterium of lactic acid.The bacterium can not only produce the irritating and soft fine and smooth flue gas effect of reduction cigarette during the fermentation
Lactic acid, moreover it is possible to produce assign the peculiar style of cigarette flavor substance.
The content of the invention
It is an object of the invention to provide a kind of method that fixing lactic acid bacteria prepares cigarette Semen Persicae extract, using immobilization
Lactobacillus-fermented technology, develops a kind of new Semen Persicae extract, and the extract can be used in cigarette, can enrich cigarette
Perfume (or spice), increases fragrant and sweet rhythm and the fragrant note of acid, and increase flue gas promotes the production of body fluid sense, and soft fine and smooth flue gas reduces excitant, and improvement throat is comfortable
Property.
The present invention provides a kind of method that fixing lactic acid bacteria prepares cigarette Semen Persicae extract, it is characterised in that specifically include
Following steps：
(1) peach kernel is taken, the water of 2~5 times of its weight is added, 12-24h, the refluxing extraction 2~3 at 95~100 DEG C is soaked
Secondary, each extraction time is 5~8 hours, and room temperature is cooled to after having extracted, and is filtrated to get extract solution, merges multiple extract solution,
Obtain Semen Persicae extract A；
(2) by lactobacillus inoculum into broth bouillon, cultivated under conditions of 37 DEG C of temperature, speed of agitator are 50rpm
48 hours, take nutrient solution in being centrifuged under 8000rpm, the wet thallus after centrifugation is standby；
(3) polyvinyl alcohol-Immobilization in Sodium Alginate of lactic acid bacteria：It is water-soluble with sodium alginate using polyvinyl alcohol water solution
Liquid prepares the polyvinyl alcohol containing mass percent 6%~10% and the mixing of the sodium alginate of mass percent 1%~3% is molten
The lactic acid bacteria wet thallus prepared in liquid, the step of the 2~5% of total solution volume are then added into mixed solution (2), is configured to
Mixed liquor, CaCl is added dropwise to peristaltic pump by the syringe needle in 1~5mm apertures by the mixed liquor prepared2Quality percentage
Than in the boric acid saturated solution for 1~3%, then bead is taken out and adds saturation Na by crosslinking 1h formation beads2SO4In solution
3-5h is crosslinked, PVA-SA fixing lactic acid bacteria beads, finally using aseptic water washing, dry for standby is obtained；
(4) by the fixing lactic acid bacteria bead prepared in Semen Persicae extract A and step (3) according to mass ratio 5~10:1 mixes
Close, be 30~37 DEG C in temperature, speed of agitator is 3~5d of fermentation under conditions of 1~5rpm, is subsequently placed in 4 DEG C of refrigerators and stands 7
~14d；
(5) step (4) fermentation after fermentation liquid is filtered, obtains clarified broth, zymotic fluid is freeze-dried, feed liquid thickness
For 1~2 centimetre, vacuum is 10Pa, and drying time is 6~12h；
(6) zymotic fluid after step (5) is freeze-dried, adds 80~95% alcoholic solutions of 2~4 times of its quality, sinks
Form sediment 24~48 hours, it is 0.1MPa to filter to take supernatant in pressure, and temperature is to be concentrated into relative density under conditions of 45 DEG C to be
1.20~1.25, obtain Semen Persicae extract.
The lactic acid bacteria used in the present invention is Lactobacillus plantarum (Lactobacillus plantarum) PJH05, in 2012
On January 5, in is preserved in Wuhan University's China typical culture collection center, and preserving number is：CCTCC NO：M2012002.
The immobilized microorganism technology that the present invention is used has that fermentation process efficiency high, separation of solid and liquid be easy, mithridatism is strong
The advantages of, the deficiency of traditional biological technique can be made up.
The present invention is using peach kernel as raw material, using fixing lactic acid bacteria fermentation Semen Persicae extract, obtains a kind of best in quality
Cigarette Semen Persicae extract, the extract has abundant cigarette perfume in cigarette, increases fragrant and sweet rhythm, and increase flue gas promotes the production of body fluid sense, soft
Fine and smooth flue gas, reduces excitant, improves the effect such as throat's comfortableness, moreover it is possible to assign the sour fragrant breeze lattice of cigarette characteristic.With existing peach
Benevolence medicinal extract is compared with Semen Persicae extract, and the Semen Persicae extract reduces sharp property in cigarette and the effect of soft fine and smooth flue gas is brighter
It is aobvious, while also assigning the sour fragrant breeze lattice of cigarette characteristic；Compared with suspension lactobacillus-fermented, Semen Persicae extract prepared by this method has
Machine acid content is added in 3.83%~7.42%, cigarette effect assessment, and oral cavity pleasant impression is more comfortable, and sense of promoting the production of body fluid is more preferable, fragrance
More enrich.This method instead of the traditional extractive technique of Semen Persicae extract, have updated biofermentation preparation technology so that its energy
Enough applicability increases in cigarette, the preparation to cigarette Semen Persicae extract provides a new way.
Embodiment
With reference to embodiment to further detailed description of the present invention.
The lactic acid bacteria used in the present invention is Lactobacillus plantarum (Lactobacillus plantarum) PJH05, in 2012
On January 5, in is preserved in Wuhan University's China typical culture collection center, and preserving number is：CCTCC NO：M2012002.
PVA-SA (polyvinyl alcohol-sodium alginate) fixing lactic acid bacteria bead in following examples of the present invention be according to
Lower method is obtained using polyvinyl alcohol (PVA) and sodium alginate (SA) to being fixed of lactic acid bacteria：
(1) by lactobacillus inoculum into broth bouillon, cultivated under conditions of 37 DEG C of temperature, speed of agitator are 50rpm
48 hours, take nutrient solution in being centrifuged under 8000rpm, the wet thallus after centrifugation is standby；Broth bouillon is conventional in the above method
Broth medium, its Ju Ti Pei Fang be (every liter)：Peptone 10g, beef extract powder 3g, sodium chloride 5g, final pH 7.3 ±
0.1；
(2) polyvinyl alcohol-Immobilization in Sodium Alginate of lactic acid bacteria：It is water-soluble with sodium alginate using polyvinyl alcohol water solution
Liquid prepares the mixed solution of the polyvinyl alcohol of the 6%-10% containing mass percent and mass percent 1%-3% sodium alginate,
Then the lactic acid bacteria wet thallus prepared in the step of 2-5% of total solution volume is added into mixed solution (2), is configured to mixing
Liquid, CaCl is added dropwise to peristaltic pump by the syringe needle in 1-5mm apertures by the mixed liquor prepared2Mass percent be 1-
In 3% boric acid saturated solution, then bead is taken out and adds saturation Na by crosslinking 1h formation beads2SO43- is crosslinked in solution
5h, obtains PVA-SA fixing lactic acid bacteria beads, finally using aseptic water washing, dry for standby；It is described to contain 1-3%CaCl2's
The amount and saturation Na of boric acid saturated solution2SO4The amount of solution has no particular/special requirement, contains 1-3%CaCl2Boric acid saturated solution
Only need to that mixed liquor can be made to produce precipitation, meet the immobilization requirement of lactic acid bacteria, Na2SO4Solution is only needed to bead
It is completely soaked and just may be used；The boric acid saturated solution and saturation Na2SO4Solution is saturated aqueous solution.
Embodiment 1, a kind of method that fixing lactic acid bacteria prepares cigarette Semen Persicae extract, comprises the following steps：
(1) add 2 times of water by weight into peach kernel, soak 12 hours, refluxing extraction 2 times at 100 DEG C, extract every time
Time is 6 hours, and room temperature is cooled to after having extracted, and is filtrated to get extract solution, merges extract solution twice, obtains Semen Persicae extract
A, the condition extracted twice is identical；
(2) the PVA-SA fixing lactic acid bacterias bead for preparing Semen Persicae extract A and the above method is according to mass ratio 5:1 mixes
Close, be 30 DEG C in temperature, speed of agitator is the 4d that fermented under conditions of 5rpm, is subsequently placed in 4 DEG C of refrigerators and preserves 8d；
(3) step (2) fermentation after fermentation liquid is filtered, obtains clarified broth, zymotic fluid is freeze-dried, feed liquid thickness
For 1~2 centimetre, vacuum is 10Pa, and drying time is 6h；
(4) dried zymotic fluid in step (3) is taken, 95% alcoholic solution of 3 times of its quality is added, precipitated 24 hours,
It is 0.1MPa that supernatant, which is filtered to take, in pressure, and temperature is that to be concentrated into relative density under conditions of 45 DEG C be 1.2, obtains peach kernel extraction
Thing.
The Semen Persicae extract prepared in embodiment 1 has abundant cigarette perfume in cigarette, increases fragrant and sweet rhythm and the fragrant note of acid, increases
Smoke adding gas promotes the production of body fluid sense, soft fine and smooth flue gas, reduces excitant, improves throat's comfortableness.
Embodiment 2, a kind of method that fixing lactic acid bacteria prepares cigarette Semen Persicae extract, comprises the following steps：
(1) peach kernel is taken, the water of 5 times of its weight is added, soaked 16 hours, then refluxing extraction 3 times at 98 DEG C are carried every time
It is 8 hours to take the time, and room temperature is cooled to after having extracted, and is filtrated to get extract solution, merges No. three extract solutions, obtains peach kernel extraction
Thing A；The condition of three extractions is identical；
(2) Semen Persicae extract A is pressed with the PVA-SA fixing lactic acid bacteria beads that prepare in the fixation above method for preparing
According to mass ratio 8:1 mixing, is 35 DEG C in temperature, speed of agitator is the 5d that fermented under conditions of 3rpm, is subsequently placed in 4 DEG C of refrigerators and protects
Deposit 9d；
(3) step (2) fermentation after fermentation liquid is filtered, obtains clarified broth, zymotic fluid is freeze-dried, feed liquid thickness
For 1~2 centimetre, vacuum is 10Pa, and drying time is 12h；
(4) dried zymotic fluid in step (3) is taken, 95% alcoholic solution of 3 times of its quality is added, precipitated 36 hours,
It is 0.1MPa that supernatant, which is filtered to take, in pressure, and temperature is that to be concentrated into relative density under conditions of 45 DEG C be 1.2, obtains peach kernel extraction
Thing.
The Semen Persicae extract prepared in embodiment 2 has abundant cigarette perfume, increases fragrant and sweet rhythm and the fragrant note of acid, increase flue gas life
Tianjin is felt, soft fine and smooth flue gas, reduces excitant, improves throat's comfortableness.
Embodiment 3, a kind of method that fixing lactic acid bacteria prepares cigarette Semen Persicae extract, comprises the following steps：
(1) peach kernel is taken, the water of 3 times of its weight is added, soaked 24 hours, then refluxing extraction 3 times at 95 DEG C are carried every time
It is 6 hours to take the time, and room temperature is cooled to after having extracted, and is filtrated to get extract solution, merges No. three extract solutions, obtains peach kernel extraction
Thing A；Three times extraction conditions are identical；
(2) the PVA-SA fixing lactic acid bacterias bead for preparing Semen Persicae extract A and the above method is according to mass ratio 10:1
Mixing, is 37 DEG C in temperature, speed of agitator is the 3d that fermented under conditions of 2rpm, is subsequently placed in 4 DEG C of refrigerators and preserves 14d；
(3) step (2) fermentation after fermentation liquid is filtered, obtains clarified broth, zymotic fluid is freeze-dried, feed liquid thickness
For 1~2 centimetre, vacuum is 10Pa, and drying time is 10h；
(4) dried zymotic fluid in step (3) is taken, 95% alcoholic solution of 3 times of its quality is added, precipitated 24 hours,
It is 0.1MPa that supernatant, which is filtered to take, in pressure, and temperature is that to be concentrated into relative density under conditions of 45 DEG C be 1.25, obtains peach kernel and carries
Take thing.
The Semen Persicae extract prepared in embodiment 3 has abundant cigarette perfume, increases fragrant and sweet rhythm and the fragrant note of acid, increase flue gas life
Tianjin is felt, soft fine and smooth flue gas, reduces excitant, improves throat's comfortableness.
Contrast experiment, applicant carries the peach kernel that Semen Persicae extract prepared by the present invention is prepared with existing traditional extraction process
Take thing, the Semen Persicae extract prepared using suspension lactobacillus-fermented to be added to according to normal perfuming mode in pipe tobacco, cigarette be made,
And smoked panel test, its effect of smokeing panel test is as follows：
The sensory evaluation effect that peach kernel prepared by three kinds of different process is extracted in cigarette compares
It can be seen that to come from above-mentioned contrast experiment, the Semen Persicae extract for preparing is added in cigarette effect more in the present invention
It is good, and found according to specific experiment analysis, compared with suspension lactobacillus-fermented, Semen Persicae extract organic acid prepared by this method
Content is added in 3.83%~7.42%, cigarette effect assessment, and oral cavity pleasant impression is more comfortable, and fragrance more enriches.
Claims (2)
1. a kind of method that fixing lactic acid bacteria prepares cigarette Semen Persicae extract, it is characterised in that specifically include following steps：
(1) peach kernel is taken, the water of 2~5 times of its weight is added, 12-24h is soaked, refluxing extraction 2~3 times at 95~100 DEG C, often
Secondary extraction time is 5~8 hours, and room temperature is cooled to after having extracted, and is filtrated to get extract solution, merges multiple extract solution, obtains
Semen Persicae extract A；
(2) by lactobacillus inoculum into broth bouillon, culture 48 is small under conditions of 37 DEG C of temperature, speed of agitator are 50rpm
When, take nutrient solution in being centrifuged under 8000rpm, the wet thallus after centrifugation is standby；
(3) polyvinyl alcohol-Immobilization in Sodium Alginate of lactic acid bacteria：Matched somebody with somebody using polyvinyl alcohol water solution with sodium alginate aqueous solution
The mixed solution of the polyvinyl alcohol containing mass percent 6%~10% and the sodium alginate of mass percent 1%~3% is made, so
The lactic acid bacteria wet thallus prepared in the step of adding the 2~5% of total solution volume in backward mixed solution (2), is configured to mixing
Liquid, CaCl is added dropwise to peristaltic pump by the syringe needle in 1~5mm apertures by the mixed liquor prepared2Mass percent be 1
In~3% boric acid saturated solution, then bead is taken out and adds saturation Na by crosslinking 1h formation beads2SO4It is crosslinked in solution
3-5h, obtains PVA-SA fixing lactic acid bacteria beads, finally using aseptic water washing, dry for standby；
(4) by the fixing lactic acid bacteria bead prepared in the Semen Persicae extract A and step (3) that are prepared in step (1) according to quality
Than 5~10:1 mixing, is 30~37 DEG C in temperature, and speed of agitator is 3~5d of fermentation under conditions of 1~5rpm, is subsequently placed in 4 DEG C
7~14d is preserved in refrigerator；
(5) filtering fermentation liquor after step (4) fermentation refrigeration is taken, clarified broth is obtained, zymotic fluid is freeze-dried, feed liquid is thick
Spend for 1~2 centimetre, vacuum is 10Pa, drying time is 6~12h；
(6) zymotic fluid after being freeze-dried in step (5) is taken, 80~95% alcoholic solutions of 2~4 times of its quality are added, precipitated
24~48 hours, it was 0.1MPa to filter to take supernatant in pressure, and temperature is that to be concentrated into relative density under conditions of 45 DEG C be 1.20
~1.25, obtain Semen Persicae extract.
2. the method that a kind of fixing lactic acid bacteria according to claim 1 prepares cigarette Semen Persicae extract, it is characterised in that：
Lactic acid bacteria in the step (2) is Lactobacillus plantarum (Lactobacillus plantarum) PJH05CCTCC NO：
M2012002。
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108192724A (en) * | 2018-01-10 | 2018-06-22 | 湖北中烟工业有限责任公司 | A kind of preparation method and applications of fermentation type tobacco cherry extract |
| CN110693063A (en) * | 2019-11-13 | 2020-01-17 | 湖北中烟工业有限责任公司 | Preparation method of blueberry extract for cigarettes |
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|---|---|---|---|---|
| CN108192724A (en) * | 2018-01-10 | 2018-06-22 | 湖北中烟工业有限责任公司 | A kind of preparation method and applications of fermentation type tobacco cherry extract |
| CN110693063A (en) * | 2019-11-13 | 2020-01-17 | 湖北中烟工业有限责任公司 | Preparation method of blueberry extract for cigarettes |
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Application publication date: 20170718 |