CN107058279A - A kind of fixing lactic acid bacteria prepares method of the cigarette with lemon-grass extract - Google Patents

A kind of fixing lactic acid bacteria prepares method of the cigarette with lemon-grass extract Download PDF

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Publication number
CN107058279A
CN107058279A CN201610895735.4A CN201610895735A CN107058279A CN 107058279 A CN107058279 A CN 107058279A CN 201610895735 A CN201610895735 A CN 201610895735A CN 107058279 A CN107058279 A CN 107058279A
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lemon
cigarette
lactic acid
acid bacteria
solution
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CN201610895735.4A
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Chinese (zh)
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庞登红
黄龙
程书锋
陈�胜
吕品
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Wuhan Huanghelou Flavoring and Essence Co Ltd
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Wuhan Huanghelou Flavoring and Essence Co Ltd
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/10—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a carbohydrate
    • A—HUMAN NECESSITIES
    • A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
    • A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
    • A24B3/00—Preparing tobacco in the factory
    • A24B3/12—Steaming, curing, or flavouring tobacco
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/04—Enzymes or microbial cells immobilised on or in an organic carrier entrapped within the carrier, e.g. gel or hollow fibres
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/08—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
    • C12P1/04—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes by using bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/225—Lactobacillus
    • C12R2001/25—Lactobacillus plantarum

Abstract

The present invention provides a kind of fixing lactic acid bacteria and prepares method of the cigarette with lemon-grass extract.The preparation method is concretely comprised the following steps：(1) lemon-grass water extraction at 95~100 DEG C is taken repeatedly, to merge extract solution, obtain lemon-grass extract A；(2) using lactobacillus inoculum cultivate into broth bouillon centrifugation after obtain wet thallus, in the mixed solution that wet thallus is added to polyvinyl alcohol and sodium alginate, and with contain 1~3%CaCl2Boric acid saturated solution and saturation Na2SO4PVA SA fixing lactic acid bacteria beads are crosslinked to obtain in solution；(3) by lemon-grass extract A and fixing lactic acid bacteria bead according to mass ratio 5~10:1 mixing after fermentation, then carries out molecular distillation, it is cigarette lemon-grass extract to take light component after filtering, precipitation, concentration.The present invention is added in cigarette abundant cigarette perfume, and the fragrant note of increase acid lifts cigarette flavor quality, increases the cigarette flavor property sent out thoroughly, and soft fine and smooth flue gas reduces excitant, covers miscellaneous gas, improves pleasant impression, improves throat's comfortableness.

Description

A kind of fixing lactic acid bacteria prepares method of the cigarette with lemon-grass extract
Technical field
The present invention relates to cigarette additive field, and in particular to prepares cigarette lemon-grass extract using fixing lactic acid bacteria Preparation method.
Background technology
Flavoring and casing is the key link in cigarette process, is the determinant and raising cigarette flavor for improving product style One of effective means of quality.Current essence spice for cigarette is main or passes through the extraction to natural animal-plant, chemical synthesis system It is standby.Due to by natural plant resource is limited and the harsher limitation of chemical synthesis condition.With the development of biotechnology, in recent years Come, prepare cigarette flavor using microbe fermentation method, an economy, efficient approach are provided for the production of cigarette flavor, Develop the new cigarette flavor that some conventional methods can not be produced.
Immobilized microorganism technology, is to be fixed on special microorganism on the carrier of choosing card, makes its highly dense and protect Bioactivity is held, the biotechnology that can quickly, largely breed under condition of compatibility can make up suspension microorganism fermentation process The shortcomings of efficiency is low, separation of solid and liquid is difficult, mithridatism is poor.Therefore, in recent years, the research of immobilized microorganism technology is lived very much Jump, development is very fast, has application to many industrial departments such as food fermentation industry, environmental protection, new energy development and biological biography Sensor etc., and deep development is achieved, show powerful superiority.It is mainly concerned with the following aspects：In food hair It is used to produce ethanol, beer, Yoghourt etc. in ferment industry；It is used for environmental monitoring and the processing of industrial wastewater in environmental protection； It is used to produce hydrogen, methane, biochemical fuel cell, microorganism battery etc. in new energy development；Immobilized microorganism is alternatively arranged as molecule Recognition component, for making various biology sensors.And it is continuous ripe, perfect with fixation of microbe technology, gradually to height The direction such as effect low energy consumption, low cost, simplification, safety automation, pollution-free is developed, dirty in energy crisis, shortage of resources, environment In the case that dye is on the rise, it thus will be promoted more deeply and to be widely applied in terms of wastewater treatment, with more deepening Remote meaning.Especially in terms of wastewater treatment, have great application prospect.But preparing cigarette using immobilized microorganism technology Report is but had no with lemon-grass extract.
Lemon-grass, also known as Bao Mao, are grass family cogon herbaceos perennial.Lemon-grass is also that a kind of conventional cigarette is used Additive, lemon-grass medicinal extract, lemon-grass extract, lemon-grass essential oil by raw material of lemon-grass are widely used in cigarette, tool There are abundant cigarette perfume, reduction excitant and soft fine and smooth flue gas.
Lactic acid bacteria (lactic acid bacteria, LAB) is that a class can be produced largely using fermentable carbohydrate The common name of the bacterium of lactic acid.The bacterium can not only produce the irritating and soft fine and smooth flue gas effect of reduction cigarette during the fermentation Lactic acid, moreover it is possible to produce assign the peculiar style of cigarette flavor substance.
The content of the invention
Method of the cigarette with lemon-grass extract is prepared it is an object of the invention to provide a kind of fixing lactic acid bacteria, using fixation Change lactobacillus-fermented technology, develop a kind of new lemon-grass extract, the extract can be used in cigarette, Neng Goufeng Rich cigarette perfume, lifts cigarette flavor quality, increases the cigarette flavor property sent out thoroughly, and soft fine and smooth flue gas reduces excitant, improves throat and relaxes Adaptive, covers miscellaneous gas, improves pleasant impression
The present invention provides a kind of fixing lactic acid bacteria and prepares method of the cigarette with lemon-grass extract, it is characterised in that specific bag Include following steps：
(1) lemon-grass is taken, the water of 5~10 times of its weight is added, refluxing extraction 2~3 times, are carried every time at 95~100 DEG C It is 2~3 hours to take the time, and room temperature is cooled to after having extracted, and is filtrated to get extract solution, merges multiple extract solution, obtains lemongrass Careless extract A；
(2) by lactobacillus inoculum into broth bouillon, cultivated under conditions of 37 DEG C of temperature, speed of agitator are 50rpm 48 hours, take nutrient solution in being centrifuged under 8000rpm, the wet thallus after centrifugation is standby；
(3) polyvinyl alcohol-Immobilization in Sodium Alginate of lactic acid bacteria：It is water-soluble with sodium alginate using polyvinyl alcohol water solution Liquid prepares the polyvinyl alcohol containing mass percent 6%~10% and the mixing of the sodium alginate of mass percent 1%~3% is molten The lactic acid bacteria wet thallus prepared in liquid, the step of the 2~5% of total solution volume are then added into mixed solution (2), is configured to Mixed liquor, CaCl is added dropwise to peristaltic pump by the syringe needle in 1~5mm apertures by the mixed liquor prepared2Quality percentage Than in the boric acid saturated solution for 1~3%, then bead is taken out and adds saturation Na by crosslinking 1h formation beads2SO4In solution 3-5h is crosslinked, PVA-SA fixing lactic acid bacteria beads, finally using aseptic water washing, dry for standby is obtained；
(4) the lemon-grass extract A prepared in step (1) and the fixing lactic acid bacteria bead of preparation in step (3) are pressed According to mass ratio 5~10:1 mixing, is 30~37 DEG C in temperature, and speed of agitator is 2~4d of fermentation under conditions of 1~5rpm, then It is placed in 7~14d of preservation in 4 DEG C of refrigerators；
(5) by the zymotic fluid of step (4), 80~95% alcoholic solutions of 2~4 times of its quality are added, precipitation 24~48 is small When, it is 0.1MPa to filter to take supernatant in pressure, and temperature is that to be concentrated into relative density under conditions of 45 DEG C be 1.20~1.30, is obtained Lemon-grass extract B；
(6) it is 0.1~10Pa in pressure by the lemon-grass extract B of step (5), feeding temperature is 55 DEG C~75 DEG C, is entered Material speed is 2~60ml/min, and scraper plate rotating speed is carries out molecular distillation under conditions of 100~130rpm, and it is lemongrass to take light component Careless extract.
The lactic acid bacteria used in the present invention is Lactobacillus plantarum (Lactobacillus plantarum) PJH05, in 2012 On January 5, in is preserved in Wuhan University's China typical culture collection center, and preserving number is：CCTCC NO：M2012002.
The immobilized microorganism technology that the present invention is used has that fermentation process efficiency high, separation of solid and liquid be easy, mithridatism is strong The advantages of, the deficiency of traditional biological technique can be made up.
The present invention, using fixing lactic acid bacteria fermentation lemon-grass extract, obtains a kind of product using the lemongrass showy flowers of herbaceous plants as raw material Good cigarette lemon-grass extract of fine quality, the extract has abundant cigarette perfume in cigarette, lifts cigarette flavor quality, increase volume The cigarette perfume gas property sent out thoroughly, soft fine and smooth flue gas reduces excitant, covers miscellaneous gas, improves pleasant impression, improves the effect such as throat's comfortableness, The sour fragrant breeze lattice of cigarette characteristic are also assigned simultaneously；Compared with suspension lactobacillus-fermented, lemon-grass extract prepared by this method has Machine acid content is added in 2.48%~6.59%, cigarette effect assessment, and oral cavity pleasant impression is more comfortable, and fragrance more enriches.Should Method instead of the traditional extractive technique of lemon-grass extract, have updated biofermentation preparation technology so that it can be in cigarette Middle applicability increase, a new way is provided to cigarette with the preparation of lemon-grass extract.
Embodiment
With reference to embodiment to further detailed description of the present invention.
The lactic acid bacteria used in the present invention is Lactobacillus plantarum (Lactobacillus plantarum) PJH05, in 2012 On January 5, in is preserved in Wuhan University's China typical culture collection center, and preserving number is：CCTCC NO：M2012002.
PVA-SA (polyvinyl alcohol-sodium alginate) fixing lactic acid bacteria bead in following examples of the present invention be according to Lower method is obtained using polyvinyl alcohol (PVA) and sodium alginate (SA) to being fixed of lactic acid bacteria：
(1) by lactobacillus inoculum into broth bouillon, cultivated under conditions of 37 DEG C of temperature, speed of agitator are 50rpm 48 hours, take nutrient solution in being centrifuged under 8000rpm, the wet thallus after centrifugation is standby；Broth bouillon is conventional in the above method Broth medium, its specific formula be (every liter)：Peptone 10g, beef extract powder 3g, sodium chloride 5g, final pH 7.3 ± 0.1；
(2) polyvinyl alcohol-Immobilization in Sodium Alginate of lactic acid bacteria：It is water-soluble with sodium alginate using polyvinyl alcohol water solution Liquid prepares the mixed solution of the polyvinyl alcohol of the 6%-10% containing mass percent and mass percent 1%-3% sodium alginate, Then the lactic acid bacteria wet thallus prepared in the step of 2-5% of total solution volume is added into mixed solution (2), is configured to mixing Liquid, CaCl is added dropwise to peristaltic pump by the syringe needle in 1-5mm apertures by the mixed liquor prepared2Mass percent be 1- In 3% boric acid saturated solution, then bead is taken out and adds saturation Na by crosslinking 1h formation beads2SO43- is crosslinked in solution 5h, obtains PVA-SA fixing lactic acid bacteria beads, finally using aseptic water washing, dry for standby；It is described to contain 1-3%CaCl2's The amount and saturation Na of boric acid saturated solution2SO4The amount of solution has no particular/special requirement, contains 1-3%CaCl2Boric acid saturated solution Only need to that mixed liquor can be made to produce precipitation, meet the immobilization requirement of lactic acid bacteria, Na2SO4Solution is only needed to bead It is completely soaked and just may be used；The boric acid saturated solution and full, Na2SO4Solution is saturated aqueous solution.
A kind of embodiment 1, fixing lactic acid bacteria prepares method of the cigarette with lemon-grass extract, comprises the following steps：
(1) 5 times of water, refluxing extraction 2 times at 100 DEG C are added by weight into lemon-grass, each extraction time is 3 small When, room temperature is cooled to after having extracted, and extract solution is filtrated to get, merge extract solution twice, obtain lemon-grass extract A, twice The condition of extraction is identical；
(2) it is the lemon-grass extract A of preparation in step (1) and PVA-SA fixing lactic acid bacterias prepared by the above method is small Ball is according to mass ratio 5:1 mixing, is 30 DEG C in temperature, speed of agitator is the 4d that fermented under conditions of 5rpm, is subsequently placed in 4 DEG C of refrigerators Middle standing 7d；
(3) step (2) fermentation after fermentation liquid is filtered, obtains clarified broth, 95% alcohol for adding 3 times of its quality is molten Liquid, is precipitated 24 hours, and it is 0.1MPa to filter to take supernatant in pressure, and temperature is to be concentrated into relative density under conditions of 45 DEG C to be 1.25, obtain lemon-grass extract B；
(4) the lemon-grass extract B of step (3) is being subjected to molecular distillation, it is lemon-grass extract to take light component；Its point Son distillation parameter be：Pressure is 5Pa, and feeding temperature is 65 DEG C, and charging rate is 30ml/min, and scraper plate rotating speed is 100rpm.
The lemon-grass extract prepared in embodiment 1, which is added in cigarette, enriches cigarette perfume, the fragrant note of increase acid, lifts cigarette Aroma quality, increases the cigarette flavor property sent out thoroughly, and soft fine and smooth flue gas reduces excitant, covers miscellaneous gas, improves pleasant impression, improves larynx Portion's comfortableness.
A kind of embodiment 2, fixing lactic acid bacteria prepares method of the cigarette with lemon-grass extract, comprises the following steps：
(1) the lemongrass showy flowers of herbaceous plants is taken, the water of 8 times of its weight is added, refluxing extraction 3 times at 97 DEG C, each extraction time is 2 small When, room temperature is cooled to after having extracted, and extract solution is filtrated to get, merge No. three extract solutions, obtain lemon-grass extract A；Three times The condition of extraction is identical；
(2) the PVA-SA fixing lactic acid bacteria beads that will be prepared in lemon-grass extract A and the fixation above method prepared According to mass ratio 8:1 mixing, is 35 DEG C in temperature, speed of agitator is the 3d that fermented under conditions of 3rpm, is subsequently placed in 4 DEG C of refrigerators Preserve 10d；
(3) step (3) fermentation after fermentation liquid is filtered, obtains clarified broth, 80% alcohol for adding 4 times of its quality is molten Liquid, is precipitated 48 hours, and it is 0.1MPa to filter to take supernatant in pressure, and temperature is to be concentrated into relative density under conditions of 45 DEG C to be 1.20, obtain lemon-grass extract B；
(4) the lemon-grass extract B of step (3) is being subjected to molecular distillation, it is lemon-grass extract to take light component；Its point Son distillation parameter be：Pressure is 2Pa, and feeding temperature is 70 DEG C, and charging rate is 40ml/min, and scraper plate rotating speed is 120rpm.
The lemon-grass extract prepared in embodiment 2, which is added in cigarette, enriches cigarette perfume, the fragrant note of increase acid, lifts cigarette Aroma quality, increases the cigarette flavor property sent out thoroughly, and soft fine and smooth flue gas reduces excitant, covers miscellaneous gas, improves pleasant impression, improves larynx Portion's comfortableness.
A kind of embodiment 3, fixing lactic acid bacteria prepares method of the cigarette with lemon-grass extract, comprises the following steps：
(1) the lemongrass showy flowers of herbaceous plants is taken, the water of 10 times of its weight is added, refluxing extraction 3 times at 95 DEG C, each extraction time is 3 Hour, room temperature is cooled to after having extracted, and extract solution is filtrated to get, merge No. three extract solutions, obtain lemon-grass extract A；Three Secondary extraction conditions are identical；
(2) the PVA-SA fixing lactic acid bacterias bead for preparing lemon-grass extract A and the above method is according to mass ratio 10: 1 mixing, is 37 DEG C in temperature, speed of agitator is the 4d that fermented under conditions of 1rpm；
(3) step (2) fermentation after fermentation liquid is filtered, obtains clarified broth, 95% alcohol for adding 2 times of its quality is molten Liquid, is precipitated 48 hours, and it is 0.1MPa to filter to take supernatant in pressure, and temperature is to be concentrated into relative density under conditions of 45 DEG C to be 1.3, obtain lemon-grass extract B；
(4) the lemon-grass extract B of step (3) is being subjected to molecular distillation, it is lemon-grass extract to take light component；Its point Son distillation parameter be：Pressure is 8Pa, and feeding temperature is 60 DEG C, and charging rate is 15ml/min, and scraper plate rotating speed is 130rpm.
The lemon-grass extract prepared in embodiment 3, which is added in cigarette, enriches cigarette perfume, the fragrant note of increase acid, lifts cigarette Aroma quality, increases the cigarette flavor property sent out thoroughly, and soft fine and smooth flue gas reduces excitant, covers miscellaneous gas, improves pleasant impression, improves larynx Portion's comfortableness.
Contrast experiment, the lemongrass that the lemon-grass extract that applicant prepares the present invention is prepared with existing traditional extraction process Careless extract, the lemon-grass extract prepared using suspension lactobacillus-fermented are added in pipe tobacco according to normal perfuming mode, are made Into cigarette, and smoked panel test, its effect of smokeing panel test is as follows：
The sensory evaluation effect that lemon-grass prepared by three kinds of different process is extracted in cigarette compares
It can be seen that to come from above-mentioned contrast experiment, the lemon-grass extract for preparing is added in cigarette effect more in the present invention It is good, and found according to specific experiment analysis, compared with suspension lactobacillus-fermented, lemon-grass extract prepared by this method is organic Acid content is added in 2.48%~6.59%, cigarette effect assessment, and oral cavity pleasant impression is more comfortable, and fragrance more enriches.

Claims (2)

1. a kind of fixing lactic acid bacteria prepares method of the cigarette with lemon-grass extract, it is characterised in that specifically include following steps：
(1) lemon-grass is taken, the water of 5~10 times of its weight is added, refluxing extraction 2~3 times at 95~100 DEG C, when extracting every time Between be 2~3 hours, be cooled to room temperature after having extracted, and be filtrated to get extract solution, the multiple extract solution of merging obtains lemon-grass and carried Take thing A；
(2) by lactobacillus inoculum into broth bouillon, culture 48 is small under conditions of 37 DEG C of temperature, speed of agitator are 50rpm When, take nutrient solution in being centrifuged under 8000rpm, the wet thallus after centrifugation is standby；
(3) polyvinyl alcohol-Immobilization in Sodium Alginate of lactic acid bacteria：Matched somebody with somebody using polyvinyl alcohol water solution with sodium alginate aqueous solution The mixed solution of the polyvinyl alcohol containing mass percent 6%~10% and the sodium alginate of mass percent 1%~3% is made, so The lactic acid bacteria wet thallus prepared in the step of adding the 2~5% of total solution volume in backward mixed solution (2), is configured to mixing Liquid, CaCl is added dropwise to peristaltic pump by the syringe needle in 1~5mm apertures by the mixed liquor prepared2Mass percent be 1 In~3% boric acid saturated solution, then bead is taken out and adds saturation Na by crosslinking 1h formation beads2SO4It is crosslinked in solution 3-5h, obtains PVA-SA fixing lactic acid bacteria beads, finally using aseptic water washing, dry for standby；
(4) by the middle fixing lactic acid bacteria bead prepared of the lemon-grass extract A prepared in step (1) and step (3) according to matter Measure ratio 5~10:1 mixing, is 30~37 DEG C in temperature, and speed of agitator is 2~4d of fermentation under conditions of 1~5rpm, is subsequently placed in 4 7~14d is preserved in DEG C refrigerator；
(5) zymotic fluid after refrigerating in step (4) is taken, 80~95% alcoholic solutions of 2~4 times of its quality are added, precipitation 24~ 48 hours, it was 0.1MPa to filter to take supernatant in pressure, temperature be concentrated under conditions of 45 DEG C relative density be 1.20~ 1.30, obtain lemon-grass extract B；
(6) by the lemon-grass extract B of step (5) in 0.1~10Pa of pressure, feeding temperature is 55 DEG C~75 DEG C, charging rate For 2~60ml/min, scraper plate rotating speed is carries out molecular distillation under conditions of 100~130rpm, and it is that lemon-grass is extracted to take light component Thing.
2. a kind of fixing lactic acid bacteria according to claim 1 prepares method of the cigarette with lemon-grass extract, its feature exists Lactic acid bacteria in the step (2) is Lactobacillus plantarum (Lactobacillus plantarum) PJH05CCTCC NO： M2012002。
CN201610895735.4A 2016-10-13 2016-10-13 A kind of fixing lactic acid bacteria prepares method of the cigarette with lemon-grass extract Pending CN107058279A (en)

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Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102505036A (en) * 2011-11-23 2012-06-20 天津科技大学 Method for improving monascus pigment color value by using immobilized lactic acid bacteria
CN105062677A (en) * 2015-07-31 2015-11-18 湖北中烟工业有限责任公司 Method for preparing fermented beet extract through processing beet juice by Lactobacillus plantarum

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102505036A (en) * 2011-11-23 2012-06-20 天津科技大学 Method for improving monascus pigment color value by using immobilized lactic acid bacteria
CN105062677A (en) * 2015-07-31 2015-11-18 湖北中烟工业有限责任公司 Method for preparing fermented beet extract through processing beet juice by Lactobacillus plantarum

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
刘丽芬: "香茅草产香菌发酵液的挥发性成分分析及在卷烟中的应用", 《烟草科技》 *

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Application publication date: 20170818