CN102952833A - Agrocybe cylindracea polysaccharide and application thereof - Google Patents
Agrocybe cylindracea polysaccharide and application thereof Download PDFInfo
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- CN102952833A CN102952833A CN2012104197791A CN201210419779A CN102952833A CN 102952833 A CN102952833 A CN 102952833A CN 2012104197791 A CN2012104197791 A CN 2012104197791A CN 201210419779 A CN201210419779 A CN 201210419779A CN 102952833 A CN102952833 A CN 102952833A
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- polysaccharide
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Abstract
The invention relates to an agrocybe cylindracea polysaccharide and application thereof, and belongs to technical field of aroma enhancement and moisture retaining of cigarette. The agrocybe cylindracea polysaccharide provided by the invention is produced by steps of: test-tube slant seed culture of a producing strain, liquid seed culture, fermentation culture and fungal polysaccharide preparation. The producing strain is Agrocybe aegerita (Agrocybe aegirit) Mo-Aa, and is preserved in China General Microbiological Culture Collection Center on October 8, 2012, and has a preservation number of CGMCC No.6651. The aroma enhancement polysaccharide provided by the invention can be used as a humectant in Chinese-style cigarette processing, has the characteristics of strong moisture retaining property, high safety, no negative impact on the original flavor of the cigarette, and is a natural humectant material for substituting a chemical humectant in high-end Chinese cigarette product processing.
Description
Technical field:
The present invention relates to a kind of Methods of Polysaccharide From Agrocybe Chaxingu and application thereof, belong to cigarette flavouring humectation technical field.
Background technology:
Make brokenly for reducing in the production of cigarettes, reduce the flue gas drying sense of smoking property of cigarette, strengthen the degree of promoting the production of body fluid of flue gas pleasant impression, need in tobacco, add humectant, to improve the physical and chemical performance of tobacco.Humectant commonly used in the present cigarette industry of China is the polyhydroxy substances such as glycerine, propylene glycol, sorbyl alcohol, these humectants mainly produce the humectation effect by its water absorbability to tobacco, and then effect is not ideal for the improvement of keeping, sucking comfort level of finished cigarettes water ratio.
Polysaccharide extensively is present in plant, animal and the microorganism cultures.By sources different, can be divided into fungus polysaccharide, vegetable polysaccharides, animal polysaccharide, algal polysaccharides and bacterial polysaccharides.The plurality of advantages such as polysaccharide has wide material sources, and natural, nontoxic, sustainable, safety in utilization is good.Polysaccharide molecule is amorphous structure, the molecule interaction has formed fine and close tridimensional network, fine and close reticulated structure is so that polysaccharide membrane has fabulous protection against the tide and water retention property just, and therefore, polysaccharide and glycitols (monose) also are often used as the cigarette humectant.
Yi Manjin etc.
[1]Report malto-oligosaccharide alcohol, NCS, the polysaccharide such as D-galactopyranose aldehydic acid, PDSI-101 and chitosan derivatives all have performance of keeping humidity and to the humectation effect of pipe tobacco.Chen Jianjun
[2]Report that natural piperylene can slow down moisture in cut tobacco fast lost or excessive absorption and flavor matter volatilization effectively, the quality of smokeing panel test is better than contrast.The positive root of Check
[3]The report tamarind seed polysaccharide can not only play good adhesive effect as the tackiness agent of reconstituted tobacco, can also improve its water tolerance.Zhu Zunquan
[4]Hijiki polysaccharide is joined on the cigarette shreds with feed liquid as cigarette, when burning and sucking, cigarette can produce distinctive chemical composition, obviously improve or improve the quality of cigarette, have the effect of promoting the production of body fluid significantly and certain relieving cough function, and can reduce the content of some objectionable constituent in the cigarette mainstream flue gas.Liu Yang etc.
[5]The report cactus polyoses adds and has preferably humectation effect, can reduce under low moisture conditions the percentage of water loss of pipe tobacco, slows down scattering and disappearing of moisture, and can increase perfume quantity, improves the flue gas sophistication, strengthens back sweet sense, alleviates the effects such as the gas of mixing.
The effects such as that edible fungi polysaccharide has is antitumor, immunomodulatory, hypotensive, reducing blood-fat, antithrombotic, promoting digestion and protecting liver
[6], have broad application prospects in industries such as medicine, health care, food-drink processing.The Cordyceps polysaccharide that studies show that take fruit bat as model animals can effectively reduce cigarette to the negative impact of life span of drosophila melanogaster
[7], shown that fungus polysaccharide is as the application prospect of additive at Cigarette processing.Yet the research of this respect both at home and abroad is close to blank.Style characteristic for Chinese-style cigarette, take edible fungus as resource, the suitable fungus polysaccharide of screening produces bacterium, optimize zymotechnique and the purifying technique of fungus polysaccharide, the novel polysaccharide class humectant that exploitation can significantly promote the cigarette humid keeping performance has important research and using value, can provide more technical support for promoting the Chinese-style cigarette humid keeping performance.
Reference:
1. easily brocade is full. and the Performance Ratio of several humectants is. tobacco science and technology, 2006,4:28-29.
2. Chen Jianjun etc. the performance of two-way humectant and in cigarette, using. Chinese tobacco journal, 2008,14:21-24.
3. the positive root of Check etc. the preparation of tamarind seed polysaccharide and the applied research in reconstituted tobacco. tobacco science and technology, 2002,7:3-5.
4. Zhu's honor power recklessly has and holds etc. Hijiki polysaccharide and the application in tobacco [P] thereof. and 2006, CN 200610128282.
5. Liu Yang etc. the extraction of cactus polyoses and the application in cigarette thereof. tobacco science and technology, 2010,10:8-11.
6. Yuan Wei etc. the extraction progress of hypha,hyphae polysaccharide. Anhui Normal University's journal, 2008,318:576-579.
7. safe and comfortable etc. it is old and feeble and alleviate the research of cigarette toxic action that Chinese caterpillar fungus extracellular polysaccharide delays fruit bat. and Aged in China is learned magazine, and 2010,30 (1): 232-233.
Summary of the invention:
The object of the present invention is to provide strong, the safe Methods of Polysaccharide From Agrocybe Chaxingu of a kind of performance of keeping humidity and application thereof.
The present invention specifically is achieved in that
The present invention utilizes CA substratum (NaNo
33g, K
2HPO
41g, KCl 0.5g, MgSO
47H
2O 0.5g, FeSO
47H
2O 0.01g, sucrose 30g, agar 18g, moisturizing is to 1000ml), by the sporidium catapult technique, from the fruit body of edible fungi of Ailaoshan, Yunnan collection, be separated to tea tree mushroom Agrocybe aegirit Mo-Aa bacterial strain.Mo-Po has been stored in China Microbial Culture Preservation Commission common micro-organisms center; Address: China. No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City; Preservation date: on October 8th, 2012; Preserving number: CGMCC No.6651.
Agrocybe aegirit Mo-Aa is 28 ℃ of lower cultivations 7 days on the CA substratum, and bacterium colony is circular, and is white to canescence, diameter 50-65mm, and bacterium colony surface fine hair shape, the bacterium colony back side is filbert.Utilize primer 5 '-TCCTCCGCTTATTGATATGC ', 5 '-GGAAGTAAAAGTCGTAACAAGG-3 ' expands the ITS4 gene of this bacterial strain through PCR, the sequence that obtains is submitted to the GeneBank public database, its sequence number is JX965388, and this sequence is to identify the principal character foundation of this bacterial strain.
Utilize the Methods of Polysaccharide From Agrocybe Chaxingu of the mycelium preparation of Mo-Aa bacterial strain artificial culture to be applied in the Chinese-style cigarette processing as humectant.
The present invention have performance of keeping humidity strong, safe, to the characteristics of the original local flavor of cigarette without negative impact, be the natural humectation material of instead of chemical humectant in the process for processing of Chinese-style cigarette high-end product.
Description of drawings:
Fig. 1 shows pipe tobacco 22 ℃ of temperature, and the percentage of water loss during relative humidity 40% changes.
Embodiment:
1, producing bacterial strain is tea tree mushroom (Agrocybe aegirit) Mo-Aa, and Mo-Po has been stored in China Microbial Culture Preservation Commission common micro-organisms center; Address: China. No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City; Preservation date: on October 8th, 2012; Preserving number: CGMCC No.6651.
2. the cultivation of Agrocybe aegirit Mo-Aa bacterial strain
Mo-Aa bacterial strain test tube slant seed culture: the thalline of this bacterial strain is inoculated into (the peeling potato 200g chopping of PDA substratum, cross leaching filtrate after 30 minutes with water boil, sucrose 20g, agar 20g, moisturizing to 1000 ml, the pH nature) on the inclined-plane, 20-30 ℃ of lower the cultivation after 4-8 days obtains the inclined-plane seed, and suitable culture condition is 28 ℃ of lower cultivations 6 days.
The cultivation of Mo-Aa strain liquid seed: the inclined-plane seed is inoculated in the PDB substratum (PDA does not add agar) in the triangular flask, and 25-30 ℃, shaking table is cultivated and was obtained in 4-8 days under the 50-250 rpm condition.Optimal culture conditions is 28 ℃, cultivates 4 days under the 200 rpm conditions.
A large amount of cultivations of Mo-Aa bacterial strain: rise in the fermentor tank at 20-1000, with the 3-8%(V/V of liquid seeds in the substratum cumulative volume) in the ratio access fermention medium, the prescription of fermention medium is sucrose 60g, saltpetre 2g, yeast extract paste 20g, MgSO
47H
2O 2g, K
2HPO
40.04g, FeSO
47H
2O 0.02g, water 1000ml, pH 6.8; The culture condition of fermentor tank is temperature 26-32 ℃, stirring velocity 150-250 rpm, fermentation time 4-8 days; Optimal culture conditions is 5% inoculum size, 28 ℃, cultivates 5 days under the 200 rpm conditions.
2. the preparation of Agrocybe aegirit Mo-Aa fungus polysaccharide
The bulk fermentation product of Mo-Aa bacterial strain obtains mycelium through Plate Filtration, under 10000-15000rpm, smashed to pieces mycelia 30-60 minute with the high-efficiency tissue stamp mill, 25-35 by mycelia weight doubly adds tap water, then in being 70-80 ℃ fireless cooker, water temperature kept 2-4 hour, filter to remove the bacterium slag, what then obtain behind the centrifugal removal moisture under 8000-10000rpm is precipitated as Crude polysaccharides.The Crude polysaccharides sample is dissolved in hot water, and the volume of pressing polysaccharide soln 1/5 adds chloroform, then adds the propyl carbinol of chloroform volume 1/5, and vibration is 20 minutes after mixing.Protein and chloroform-propyl carbinol generates gel, and centrifugal 20 minutes of 8000rpm collects supernatant liquor; Till repeatable operation 2-3 time is not muddy to chloroform-n-butanol layer.Then add 4 ℃ of refrigerator overnight of 95% ethanol juxtaposition in supernatant liquor, centrifugal collecting precipitation was dried to constant weight for 60 ℃ and was namely obtained Methods of Polysaccharide From Agrocybe Chaxingu next day.
3. Methods of Polysaccharide From Agrocybe Chaxingu is to the humectation effect test of pipe tobacco
Take by weighing the Methods of Polysaccharide From Agrocybe Chaxingu sample by 3 ‰ of pipe tobacco weight, and be dissolved in the water of pipe tobacco weight 20%, send forth equably on pipe tobacco with micro sprayer, then with the tobacco sample of adding Methods of Polysaccharide From Agrocybe Chaxingu balance 48 hours under the condition of 22 ℃ of temperature, relative humidity 60%, place afterwards the moisture eliminator of 22 ℃ of temperature, relative humidity 40%.Compare three repetitions of every processing with the processing of adding equivalent propylene glycol chemistry humectant and the sample that does not add any humectation material.The weighing example weight calculates its percentage of water loss at regular intervals, and then analyze to add the moisture-absorbing moisture-keeping performance of pipe tobacco behind the Methods of Polysaccharide From Agrocybe Chaxingu.
Test-results (Fig. 1): be under 40% the low humidity condition, not add in the blank sample of humectation material in 22 ℃ of temperature, relative humidity, the pipe tobacco percentage of water loss is linear increase trend in front 100 hours, is increased to 13.94% from 8%; The percentage of water loss increase is tending towards slowly in 100 ~ 200 hours, is increased to 14.88% from 13.94%, tends to balance afterwards.Add the sample of Methods of Polysaccharide From Agrocybe Chaxingu and propylene glycol, the pipe tobacco percentage of water loss increases slowly, and front 200 hours, percentage of water loss only was increased to 4.62% and 4.55% from 2.17% and 2.41%, tends to balance afterwards, maintains about 4.6%.The result shows, the performance of keeping humidity of Methods of Polysaccharide From Agrocybe Chaxingu performance is suitable with chemical humectant propylene glycol.
4. add Methods of Polysaccharide From Agrocybe Chaxingu to the effect test of cigarette sense organ
Take by weighing the Methods of Polysaccharide From Agrocybe Chaxingu sample by 3 ‰ of pipe tobacco weight, be dissolved in an amount of deionized water and spray equably with micro sprayer and be added on the pipe tobacco.Then with the pipe tobacco of add giving birth to Methods of Polysaccharide From Agrocybe Chaxingu 40 ℃ of lower balances of temperature 4 hours, under 100 ℃, dry by the fire again to water ratio be 12.5%, roll into cigarette and smoked panel test by cigarette smoking expert after 48 hours in 22 ℃ of temperature, 60% time balance of relative humidity.
Through expert group's smoking evaluation, the cigarette flavor amount of adding Methods of Polysaccharide From Agrocybe Chaxingu increases, and fragrance matter is abundant, covers assorted gas, and the oral cavity comfortableness is improved, and pleasant impression is cleaner, and flue gas is slightly sent out sweet.The result shows Methods of Polysaccharide From Agrocybe Chaxingu, and not only humid keeping performance is good, can also improve the cigarette sensory effects, and wide using value is arranged in Cigarette processing.
Claims (2)
1. Methods of Polysaccharide From Agrocybe Chaxingu, by produce bacterial strain through test tube slant seed culture, liquid seeds cultivation, fermentation culture, and the fungus polysaccharide preparation process obtain, it is characterized in that producing bacterial strain is tea tree mushroom (Agrocybe aegirit) Mo-Aa, this bacterial strain October 8 in 2012 was preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center, preserving number: CGMCC No.6651.
2. Methods of Polysaccharide From Agrocybe Chaxingu claimed in claim 1 is applied in the Chinese-style cigarette processing as humectant.
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103509132A (en) * | 2013-09-27 | 2014-01-15 | 广东中烟工业有限责任公司 | Fractional extraction method for honey pomelo peel polysaccharides, product thereof and application of product |
| CN103504463A (en) * | 2013-09-27 | 2014-01-15 | 广东中烟工业有限责任公司 | Application of shaddock ped polysaccharide to tobacco leaf moisturizing |
| CN103980374A (en) * | 2014-05-20 | 2014-08-13 | 河南中烟工业有限责任公司 | Morinda officinalis polysaccharide, extraction and purification method and application thereof as tobacco humectant |
| CN103980375A (en) * | 2014-05-20 | 2014-08-13 | 河南中烟工业有限责任公司 | Astragalus polysaccharide, extracting and purifying method and application thereof as tobacco moistening agent |
| CN104031162A (en) * | 2014-06-26 | 2014-09-10 | 河南中烟工业有限责任公司 | Ophiopogonpolysaccharide, extraction and purification method of ophiopogonpolysaccharide and application of ophiopogonpolysaccharide as tobacco humectant |
| CN104031160A (en) * | 2014-06-26 | 2014-09-10 | 河南中烟工业有限责任公司 | Gingko polysaccharide, extraction and purification method and application of gingko polysaccharide as tobacco humectant |
| CN104031161A (en) * | 2014-06-26 | 2014-09-10 | 河南中烟工业有限责任公司 | Dichondra repens forst polysaccharides, extraction and purification method of dichondra repens forst polysaccharides and application of dichondra repens forst polysaccharides as tobacco humectant |
| CN104045731A (en) * | 2014-06-26 | 2014-09-17 | 河南中烟工业有限责任公司 | Flammulina velutipes polysaccharide and extracting and purifying method thereof as well as application of flammulina velutipes polysaccharide as tobacco humectant |
| CN104432493A (en) * | 2014-11-03 | 2015-03-25 | 云南中烟工业有限责任公司 | Application of agrocybe cylindracea polysaccharide or compound of agrocybe cylindracea polysaccharide in cigarettes |
| CN113040410A (en) * | 2019-12-26 | 2021-06-29 | 中国科学院青岛生物能源与过程研究所 | Method for moisturizing cigar wrapper tobacco leaves |
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Cited By (14)
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| CN103504463B (en) * | 2013-09-27 | 2015-09-30 | 广东中烟工业有限责任公司 | The application of a kind of shaddock ped polysaccharide in tobacco leaf humectation |
| CN103504463A (en) * | 2013-09-27 | 2014-01-15 | 广东中烟工业有限责任公司 | Application of shaddock ped polysaccharide to tobacco leaf moisturizing |
| CN103509132B (en) * | 2013-09-27 | 2016-07-13 | 广东中烟工业有限责任公司 | The fractional extraction method of a kind of honey Fructus Citri grandis Pericarpium Citri grandis polysaccharide and the application of product and product thereof |
| CN103509132A (en) * | 2013-09-27 | 2014-01-15 | 广东中烟工业有限责任公司 | Fractional extraction method for honey pomelo peel polysaccharides, product thereof and application of product |
| CN103980375A (en) * | 2014-05-20 | 2014-08-13 | 河南中烟工业有限责任公司 | Astragalus polysaccharide, extracting and purifying method and application thereof as tobacco moistening agent |
| CN103980374A (en) * | 2014-05-20 | 2014-08-13 | 河南中烟工业有限责任公司 | Morinda officinalis polysaccharide, extraction and purification method and application thereof as tobacco humectant |
| CN104031160A (en) * | 2014-06-26 | 2014-09-10 | 河南中烟工业有限责任公司 | Gingko polysaccharide, extraction and purification method and application of gingko polysaccharide as tobacco humectant |
| CN104031161A (en) * | 2014-06-26 | 2014-09-10 | 河南中烟工业有限责任公司 | Dichondra repens forst polysaccharides, extraction and purification method of dichondra repens forst polysaccharides and application of dichondra repens forst polysaccharides as tobacco humectant |
| CN104045731A (en) * | 2014-06-26 | 2014-09-17 | 河南中烟工业有限责任公司 | Flammulina velutipes polysaccharide and extracting and purifying method thereof as well as application of flammulina velutipes polysaccharide as tobacco humectant |
| CN104031162A (en) * | 2014-06-26 | 2014-09-10 | 河南中烟工业有限责任公司 | Ophiopogonpolysaccharide, extraction and purification method of ophiopogonpolysaccharide and application of ophiopogonpolysaccharide as tobacco humectant |
| CN104031162B (en) * | 2014-06-26 | 2016-10-05 | 河南中烟工业有限责任公司 | Radix Ophiopogonis polysaccharide, method for extraction and purification and the application as tobacco humectant thereof |
| CN104432493A (en) * | 2014-11-03 | 2015-03-25 | 云南中烟工业有限责任公司 | Application of agrocybe cylindracea polysaccharide or compound of agrocybe cylindracea polysaccharide in cigarettes |
| CN113040410A (en) * | 2019-12-26 | 2021-06-29 | 中国科学院青岛生物能源与过程研究所 | Method for moisturizing cigar wrapper tobacco leaves |
| CN113040410B (en) * | 2019-12-26 | 2022-11-18 | 中国烟草总公司海南省公司 | Method for moisturizing cigar wrapper tobacco leaves |
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Application publication date: 20130306 |