CN106434828A - Preparation method of fermented parochetus communis perfumes for cigarettes - Google Patents
Preparation method of fermented parochetus communis perfumes for cigarettes Download PDFInfo
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- CN106434828A CN106434828A CN201611072049.3A CN201611072049A CN106434828A CN 106434828 A CN106434828 A CN 106434828A CN 201611072049 A CN201611072049 A CN 201611072049A CN 106434828 A CN106434828 A CN 106434828A
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- flos caraganae
- caraganae sinicae
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- 238000002360 preparation method Methods 0.000 title claims abstract description 16
- 235000019504 cigarettes Nutrition 0.000 title abstract description 24
- 239000002304 perfume Substances 0.000 title abstract description 8
- 244000161950 Parochetus communis Species 0.000 title abstract description 6
- 235000002637 Nicotiana tabacum Nutrition 0.000 claims abstract description 37
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 31
- 241000196324 Embryophyta Species 0.000 claims abstract description 20
- 241000186660 Lactobacillus Species 0.000 claims abstract description 15
- 229940039696 lactobacillus Drugs 0.000 claims abstract description 15
- 230000001954 sterilising effect Effects 0.000 claims abstract description 14
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims abstract description 11
- 241000588264 Rhizopus javanicus Species 0.000 claims abstract description 11
- 239000008103 glucose Substances 0.000 claims abstract description 11
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims abstract description 10
- 239000000706 filtrate Substances 0.000 claims abstract description 9
- 239000000047 product Substances 0.000 claims abstract description 7
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims abstract description 5
- 229910052921 ammonium sulfate Inorganic materials 0.000 claims abstract description 5
- 235000011130 ammonium sulphate Nutrition 0.000 claims abstract description 5
- WRUGWIBCXHJTDG-UHFFFAOYSA-L magnesium sulfate heptahydrate Chemical compound O.O.O.O.O.O.O.[Mg+2].[O-]S([O-])(=O)=O WRUGWIBCXHJTDG-UHFFFAOYSA-L 0.000 claims abstract description 5
- 239000011780 sodium chloride Substances 0.000 claims abstract description 5
- 229940061634 magnesium sulfate heptahydrate Drugs 0.000 claims abstract description 4
- 238000010992 reflux Methods 0.000 claims abstract description 3
- 241000628997 Flos Species 0.000 claims description 50
- 241000208125 Nicotiana Species 0.000 claims description 34
- 239000002609 medium Substances 0.000 claims description 26
- 238000001994 activation Methods 0.000 claims description 21
- 230000004913 activation Effects 0.000 claims description 20
- 239000001963 growth medium Substances 0.000 claims description 17
- 229920001817 Agar Polymers 0.000 claims description 15
- 239000008272 agar Substances 0.000 claims description 15
- 241000235645 Pichia kudriavzevii Species 0.000 claims description 14
- 239000012530 fluid Substances 0.000 claims description 12
- 229940041514 candida albicans extract Drugs 0.000 claims description 9
- 239000012138 yeast extract Substances 0.000 claims description 9
- 108090000790 Enzymes Proteins 0.000 claims description 8
- 102000004190 Enzymes Human genes 0.000 claims description 8
- 239000012153 distilled water Substances 0.000 claims description 8
- 229940088598 enzyme Drugs 0.000 claims description 8
- 238000006243 chemical reaction Methods 0.000 claims description 7
- 239000002994 raw material Substances 0.000 claims description 7
- 230000003213 activating effect Effects 0.000 claims description 6
- 239000000284 extract Substances 0.000 claims description 6
- 239000007788 liquid Substances 0.000 claims description 6
- 241000894006 Bacteria Species 0.000 claims description 5
- 238000000855 fermentation Methods 0.000 claims description 5
- 230000004151 fermentation Effects 0.000 claims description 5
- 108010059892 Cellulase Proteins 0.000 claims description 4
- 229940106157 cellulase Drugs 0.000 claims description 4
- 239000002054 inoculum Substances 0.000 claims description 4
- 238000005374 membrane filtration Methods 0.000 claims description 4
- 229920000742 Cotton Polymers 0.000 claims description 3
- 239000001888 Peptone Substances 0.000 claims description 3
- 108010080698 Peptones Proteins 0.000 claims description 3
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 3
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 claims description 3
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 3
- 229930006000 Sucrose Natural products 0.000 claims description 3
- 239000006071 cream Substances 0.000 claims description 3
- YXVFQADLFFNVDS-UHFFFAOYSA-N diammonium citrate Chemical compound [NH4+].[NH4+].[O-]C(=O)CC(O)(C(=O)O)CC([O-])=O YXVFQADLFFNVDS-UHFFFAOYSA-N 0.000 claims description 3
- 238000011081 inoculation Methods 0.000 claims description 3
- 235000019319 peptone Nutrition 0.000 claims description 3
- 239000000843 powder Substances 0.000 claims description 3
- 108010009004 proteose-peptone Proteins 0.000 claims description 3
- 238000007790 scraping Methods 0.000 claims description 3
- 239000001632 sodium acetate Substances 0.000 claims description 3
- 235000017281 sodium acetate Nutrition 0.000 claims description 3
- 239000005720 sucrose Substances 0.000 claims description 3
- 238000004821 distillation Methods 0.000 claims description 2
- 238000001976 enzyme digestion Methods 0.000 claims description 2
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 claims description 2
- 229910000359 iron(II) sulfate Inorganic materials 0.000 claims description 2
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 claims description 2
- 229920000053 polysorbate 80 Polymers 0.000 claims description 2
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims 2
- 239000011521 glass Substances 0.000 claims 2
- 239000011790 ferrous sulphate Substances 0.000 claims 1
- 235000003891 ferrous sulphate Nutrition 0.000 claims 1
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims 1
- 238000000034 method Methods 0.000 abstract description 8
- SURQXAFEQWPFPV-UHFFFAOYSA-L iron(2+) sulfate heptahydrate Chemical compound O.O.O.O.O.O.O.[Fe+2].[O-]S([O-])(=O)=O SURQXAFEQWPFPV-UHFFFAOYSA-L 0.000 abstract description 4
- 244000061176 Nicotiana tabacum Species 0.000 abstract description 3
- 239000002253 acid Substances 0.000 abstract description 3
- 150000007513 acids Chemical class 0.000 abstract description 3
- 150000001298 alcohols Chemical class 0.000 abstract description 3
- 150000001299 aldehydes Chemical class 0.000 abstract description 3
- 150000001875 compounds Chemical class 0.000 abstract description 3
- 150000002148 esters Chemical class 0.000 abstract description 3
- 239000003205 fragrance Substances 0.000 abstract description 3
- 150000002240 furans Chemical class 0.000 abstract description 3
- 150000002576 ketones Chemical class 0.000 abstract description 3
- 150000002989 phenols Chemical class 0.000 abstract description 3
- 230000002255 enzymatic effect Effects 0.000 abstract description 2
- 241000222120 Candida <Saccharomycetales> Species 0.000 abstract 1
- 108010073178 Glucan 1,4-alpha-Glucosidase Proteins 0.000 abstract 1
- 102100022624 Glucoamylase Human genes 0.000 abstract 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 abstract 1
- 239000001913 cellulose Substances 0.000 abstract 1
- 229920002678 cellulose Polymers 0.000 abstract 1
- 238000001816 cooling Methods 0.000 abstract 1
- 230000007071 enzymatic hydrolysis Effects 0.000 abstract 1
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 abstract 1
- 238000001914 filtration Methods 0.000 abstract 1
- 235000001727 glucose Nutrition 0.000 abstract 1
- 238000010438 heat treatment Methods 0.000 abstract 1
- 230000007062 hydrolysis Effects 0.000 abstract 1
- 238000006460 hydrolysis reaction Methods 0.000 abstract 1
- 230000007794 irritation Effects 0.000 abstract 1
- 239000000779 smoke Substances 0.000 abstract 1
- 239000000243 solution Substances 0.000 description 11
- KYQCOXFCLRTKLS-UHFFFAOYSA-N Pyrazine Chemical compound C1=CN=CC=N1 KYQCOXFCLRTKLS-UHFFFAOYSA-N 0.000 description 4
- 229910000396 dipotassium phosphate Inorganic materials 0.000 description 4
- 238000002290 gas chromatography-mass spectrometry Methods 0.000 description 4
- UGFAIRIUMAVXCW-UHFFFAOYSA-N Carbon monoxide Chemical compound [O+]#[C-] UGFAIRIUMAVXCW-UHFFFAOYSA-N 0.000 description 3
- 206010011224 Cough Diseases 0.000 description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 3
- 238000004458 analytical method Methods 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 239000003546 flue gas Substances 0.000 description 3
- 241000411851 herbal medicine Species 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 230000000813 microbial effect Effects 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 241001061906 Caragana Species 0.000 description 2
- 208000015817 Infant Nutrition disease Diseases 0.000 description 2
- PCNDJXKNXGMECE-UHFFFAOYSA-N Phenazine Natural products C1=CC=CC2=NC3=CC=CC=C3N=C21 PCNDJXKNXGMECE-UHFFFAOYSA-N 0.000 description 2
- 239000011324 bead Substances 0.000 description 2
- 238000011953 bioanalysis Methods 0.000 description 2
- 230000036983 biotransformation Effects 0.000 description 2
- 239000008280 blood Substances 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 230000007812 deficiency Effects 0.000 description 2
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 description 2
- 208000002173 dizziness Diseases 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 238000011156 evaluation Methods 0.000 description 2
- 239000000796 flavoring agent Substances 0.000 description 2
- 235000019634 flavors Nutrition 0.000 description 2
- 239000007789 gas Substances 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 230000004060 metabolic process Effects 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 210000000214 mouth Anatomy 0.000 description 2
- 230000000391 smoking effect Effects 0.000 description 2
- 150000003505 terpenes Chemical class 0.000 description 2
- 235000007586 terpenes Nutrition 0.000 description 2
- 241000478830 Blastophysa rhizopus Species 0.000 description 1
- 244000025254 Cannabis sativa Species 0.000 description 1
- 102000004031 Carboxy-Lyases Human genes 0.000 description 1
- 108090000489 Carboxy-Lyases Proteins 0.000 description 1
- 206010019233 Headaches Diseases 0.000 description 1
- 102000004867 Hydro-Lyases Human genes 0.000 description 1
- 108090001042 Hydro-Lyases Proteins 0.000 description 1
- 102000004195 Isomerases Human genes 0.000 description 1
- 108090000769 Isomerases Proteins 0.000 description 1
- 241000134253 Lanka Species 0.000 description 1
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 1
- 102000008109 Mixed Function Oxygenases Human genes 0.000 description 1
- 108010074633 Mixed Function Oxygenases Proteins 0.000 description 1
- 102000004316 Oxidoreductases Human genes 0.000 description 1
- 108090000854 Oxidoreductases Proteins 0.000 description 1
- 102000004020 Oxygenases Human genes 0.000 description 1
- 108090000417 Oxygenases Proteins 0.000 description 1
- 241000287882 Pavo Species 0.000 description 1
- 206010037660 Pyrexia Diseases 0.000 description 1
- 241000235527 Rhizopus Species 0.000 description 1
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 1
- 208000009205 Tinnitus Diseases 0.000 description 1
- 208000006673 asthma Diseases 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 230000017531 blood circulation Effects 0.000 description 1
- VTYYLEPIZMXCLO-UHFFFAOYSA-L calcium carbonate Substances [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 description 1
- 229910000019 calcium carbonate Inorganic materials 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 239000012159 carrier gas Substances 0.000 description 1
- 238000006555 catalytic reaction Methods 0.000 description 1
- 208000013116 chronic cough Diseases 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 235000009508 confectionery Nutrition 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- -1 deaminase Proteins 0.000 description 1
- 230000006837 decompression Effects 0.000 description 1
- 229960000935 dehydrated alcohol Drugs 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 235000018927 edible plant Nutrition 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 229910052564 epsomite Inorganic materials 0.000 description 1
- 230000004438 eyesight Effects 0.000 description 1
- 231100000869 headache Toxicity 0.000 description 1
- 230000002607 hemopoietic effect Effects 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 210000000867 larynx Anatomy 0.000 description 1
- 210000004072 lung Anatomy 0.000 description 1
- 239000011777 magnesium Substances 0.000 description 1
- 229910052749 magnesium Inorganic materials 0.000 description 1
- ISPYRSDWRDQNSW-UHFFFAOYSA-L manganese(II) sulfate monohydrate Chemical compound O.[Mn+2].[O-]S([O-])(=O)=O ISPYRSDWRDQNSW-UHFFFAOYSA-L 0.000 description 1
- 208000004396 mastitis Diseases 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 235000010755 mineral Nutrition 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 238000006116 polymerization reaction Methods 0.000 description 1
- 229920000136 polysorbate Polymers 0.000 description 1
- 238000002203 pretreatment Methods 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 235000018102 proteins Nutrition 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- RDRCCJPEJDWSRJ-UHFFFAOYSA-N pyridine;1h-pyrrole Chemical class C=1C=CNC=1.C1=CC=NC=C1 RDRCCJPEJDWSRJ-UHFFFAOYSA-N 0.000 description 1
- 150000003222 pyridines Chemical class 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 238000006722 reduction reaction Methods 0.000 description 1
- 230000001953 sensory effect Effects 0.000 description 1
- VWDWKYIASSYTQR-UHFFFAOYSA-N sodium nitrate Inorganic materials [Na+].[O-][N+]([O-])=O VWDWKYIASSYTQR-UHFFFAOYSA-N 0.000 description 1
- 235000013599 spices Nutrition 0.000 description 1
- 210000000952 spleen Anatomy 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000001117 sulphuric acid Substances 0.000 description 1
- 235000011149 sulphuric acid Nutrition 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 231100000886 tinnitus Toxicity 0.000 description 1
- 238000012549 training Methods 0.000 description 1
- 230000008736 traumatic injury Effects 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P39/00—Processes involving microorganisms of different genera in the same process, simultaneously
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B9/00—Essential oils; Perfumes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
- C12P1/02—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes by using fungi
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
- C12P1/04—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes by using bacteria
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- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- General Chemical & Material Sciences (AREA)
- Mycology (AREA)
- Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention provides a preparation method of fermented parochetus communis perfumes for cigarettes. The preparation method comprises the following steps: collecting parochetus communis, adding water, sterilizing the parochetus communis at a high temperature, cooling, adjusting a pH value, adding cellulose and glucoamylase, performing the enzymatic hydrolysis in a constant-temperature table concentrator at the temperature of 40 to 60 DEG C, then adding glucose, ammonium sulfate, magnesium sulfate heptahydrate, sodium chloride and iron sulfate heptahydrate into an emzymatic hydrolysis solution, sterilizing at a high temperature, adding activated plant lactobacillus, rhizopus javanicus and candida kruse, fermenting, then adding water into a fermented product, heating, refluxing, extracting, filtering, collecting filtrate, decompressing and concentrating to obtain paste, thus obtaining the parochetus communis perfumes for the cigarettes. The perfumes for the cigarettes prepared by the method comprises various perfume compounds for tobaccos such as alcohols, aldehydes, ketone, acids, esters, phenols, and furans; and when the fermented perfumes are added into the cigarettes, offensive odor and irritation in the cigarettes can be reduced, the smoke gas is pure and mild, the fragrance of the cigarette is increased, and the remaining smell of the cigarette is relatively comfortable.
Description
Technical field
The present invention provides a kind of preparation of tobacco aromatics using, specifically a kind of preparation side of fermented type Flos Caraganae Sinicae tobacco aromatics using
Method.
Background technology
Flos Caraganae Sinicae (Parochetus communis), also known as golden peafowl, Jin Quehua, Flos Caraganae Franchetianae, hemopoietic grass (Yunnan), one
Blood etc., belongs to pulse family Papillionoideae Caragana (Caragana chinensisi Turcz.ex Maxim) plant.Mainly
It is distributed in Sichuan, Yunnan, Tibet, India, Nepal, Bhutan, Sri Lanka, Burma, Thailand, Malaysia and eastern Africa
It is distributed, is born in the hillside and shrubbery of height above sea level 1500-2000m.It is a kind of medicinal and edible plant, and root bark and flower can be used as medicine,
?《National Chinese herbal medicine compilation》、《Dictionary of medicinal plant》、《The southern regions of the Yunnan Province book on Chinese herbal medicine》With《Yunnan natural drug illustrated handbook》In all include.Goldspink
Flower is containing the composition Flos Caraganae Sinicae such as protein, fat, carbohydrate, multivitamin, several mineral materials.Its nature and flavor tepor is sweet,
Have functions that YIN nourishing and blood, spleen invigorating.Control consumptive fever cough, the dizzy soreness of waist, women asthma leucorrhea, infantile malnutrition, acute mastitis, traumatic injury
Damage etc..《The each real figure of plant is examined》Carry " YIN nourishing, YANG invigorating ".《Chinese herbal medicine is commonly used in Shanghai》Carry " promoting blood circulation by removing wind, cough-relieving, strong.Control head
Dizzy headache, tinnitus dim eyesight, chronic cough of deficiency lung, infantile malnutrition ".
Bioanalysises (or claiming biotransformation method) include microbial cell method and biological enzyme.In recent years, with biotechnology
Development with rapid changepl. never-ending changes and improvements, is also had made great progress using the work of bioanalysises production spice.Biotransformation method production cigarette perfume
The feature of material be using a variety of enzymes or microorganism, including hydrase, isomerase, deaminase, oxygenase, oxidase,
Hydroxylase and decarboxylase etc..But in prior art, using Flos Caraganae Sinicae for raw material using enzyme and microorganism composite fermentation skill
The method of art production characteristic tobacco aromatics using has no report.
Content of the invention
The present invention provides a kind of preparation method of fermented type Flos Caraganae Sinicae tobacco aromatics using, the method according to the deficiencies in the prior art
With Flos Caraganae Sinicae as raw material, in the facilitation of enzyme and microbial body under the synergism of complicated metabolism, complicated low point is formed
Sub- compound, is prepared into tobacco aromatics using, the medicated cigarette include respectively to grow tobacco in aroma component, be added in Nicotiana tabacum L., can drop
Low cigarette foreign gas, alcohol and flue gas, increase fragrance and the pleasant impression comfort level of Medicated cigarette.
The technical scheme that the present invention is provided：A kind of preparation method of fermented type Flos Caraganae Sinicae tobacco aromatics using, its feature exists
In comprising the following steps that：
(1) Flos Caraganae Sinicae is taken, according to raw material and water 1:1～1:3 ratio adds water, and sterilize at 121 DEG C 5～10min, cold
But, to room temperature, pH value is adjusted to 4.5～7.0, add the cellulase and 0.5～1.5% of Flos Caraganae Sinicae weight 0.5～1.5%
Saccharifying enzyme, react 2～5h in the constant-temperature table of 40～60 DEG C of temperature, then adjust pH value to 3.0～6.0, temperature 30～
1～3h is reacted in 55 DEG C of constant-temperature table；
(2) add according to the mass percent of Flos Caraganae Sinicae enzymolysis solution in the Flos Caraganae Sinicae enzymolysis solution in step (1) after reaction
Enter 1～5% glucose, 0.1～1% ammonium sulfate, 0.1～0.5% Magnesium sulfate heptahydrate, 0.1～1% Sodium Chloride, 0.05～
0.12% ferrous sulfate heptahydrate, then at 115 DEG C, 5～15min of sterilizing obtains the Flos Caraganae Sinicae enzymolysis solution of pretreatment；
(3) activation of fermented bacterium：
A. (from Wuhan University's China typical culture collection center, resource number is plant lactobacillus
BNCC185429 activation)：Plant lactobacillus are taken, is inoculated on ready MRS slant medium, is positioned over 30～35 DEG C
In constant incubator, 24～48h of culture makes actication of culture, then the strain of activation is inoculated in MRS fluid medium, in 30～
35 DEG C of 24～48h of constant temperature culture, obtain the plant lactobacillus seed liquor for activating；
B. Rhizopus javanicus (from Wuhan University's China typical culture collection center, resource number is BNCC183863)
Activation：Rhizopus javanicus are taken, is inoculated in ready Cha Shi yeast extract agar culture medium, be positioned over 28～30 DEG C of constant temperature training
Cultivate in foster case to spore is grown, have the mycelia of spore to the triangular flask equipped with sterilized water with inoculation shovel scraping length, add no
Bacterium bead vibrates, and is filtered with the aseptic funnel of plug Cotton Gossypii, collects filtrate, adjustment spore liquid concentration to 106Individual/ml, obtains pawl
Rhizopus seed liquor；
C. (from Wuhan University's China typical culture collection center, resource number is candida krusei
BNCC185429 activation)：Candida krusei is taken, is inoculated on ready YPD slant medium, is positioned over 30 DEG C
In constant incubator, 24～48h of culture makes actication of culture, then the strain of activation is inoculated in YPD fluid medium, in 25～
30 DEG C of 24～48h of constant temperature culture, obtain the candida krusei seed liquor for activating；
(4). the strain for having activated in step (3) is seeded to the good Flos Caraganae Sinicae of pretreatment in step (2) according to a certain amount of
In enzymolysis solution, in 25～35 DEG C of temperature, in the constant-temperature table of 100～200r/min of rotating speed, 24～72h of culture carries out fermentation reaction；
The inoculum concentration that all plants according to the mass percent of Flos Caraganae Sinicae enzymolysis solution is：Plant lactobacillus seed liquor 1～5%, Java root
Mould seed liquor 0.5～2%, candida krusei seed liquor 1～3%；
(5). the whole fermented products after fermentation in step (4) are taken, then according to fermented product compares 1 with the weight of water:0.5-1:2
Ratio add water, be heated to 85 DEG C～95 DEG C 1～2h of reflux, extract, using 0.8 μm of membrane filtration, collect filtrate, decompression
It is concentrated into paste and Flos Caraganae Sinicae tobacco aromatics using is obtained, the pressure of concentrating under reduced pressure is 0.08MPa.
The present invention preferably technical scheme：In step (1) twice enzyme digestion reaction constant-temperature table rotating speed be 100～
200r/min.
The present invention preferably technical scheme：The formula of the MRS fluid medium of activation plant lactobacillus in step (3)
As follows：10g casein peptone, 10g Carnis Bovis seu Bubali cream, 5g yeast powder, 5g glucose, 5g sodium acetate, 2g dibasic ammonium citrate, the Tween of 1g
80,2g K2HPO4, the MgSO of 0.2g4.7H2The MnSO of O, 0.05g4.H2The CaCO of O, 20g3, 1L distilled water, pH6.8；Described
MRS slant medium is addition 15g agar in the formula of MRS fluid medium；The MRS fluid medium and MRS inclined-plane
Culture medium sterilizing 20min all at 115 DEG C after preparing.
The present invention preferably technical scheme：The Cha Shi yeast extract agar culture medium of activation Rhizopus javanicus in step (3)
Formula as follows：30g sucrose, the NaNO of 3g3, the MgSO of 0.5g4·7H2The FeSO of KCl, the 0.01g of O, 0.5g4·4H2O, 1g
K2HPO4, 15g agar, 1L distilled water, pH6.0～6.5, the culture medium is with 115 DEG C of sterilizing 20min after preparing.
The present invention preferably technical scheme：In step (3), the formula of the YPD culture medium of candida krusei is such as
Under：Glucose 20g, yeast extract 10g, peptone 20g, distilled water 100mL；The YPD slant medium is to cultivate basigamy in YPD
Add 15g agar in side；The YPD culture medium and YPD slant medium are all with 115 DEG C of sterilizing 20min after preparing.
The present invention occurs to divide with Flos Caraganae Sinicae as raw material in enzymatic catalysis and microbial body under the synergism of complicated metabolism
Solution, degraded, oxidation, reduction, polymerization, be coupled, the effect such as conversion, form the tobacco aromatics using containing complicated low molecular compound,
The tobacco aromatics using includes various perfume compounds, such as alcohols, aldehydes, ketone, acids, esters, phenols, furans, Pyrazine, pyrrole
Pyridine class and terpenes etc., these aroma substances are also undoubtedly the aroma component of Nicotiana tabacum L.；It is added in Medicated cigarette, reduces Medicated cigarette
In miscellaneous QI and zest, alcohol and flue gas, increased fragrance and the relatively comfortable sense of pleasant impression of Medicated cigarette, oral cavity is cleaner, noresidue.
Specific embodiment
With reference to embodiment, the invention will be further described.Fermented bacterium activation process in the present invention is as follows：
Plant lactobacillus (from Wuhan University's China typical culture collection center, resource number is BNCC185429)
Activation：Plant lactobacillus are taken, is inoculated on ready MRS slant medium, is positioned over 30～35 DEG C of constant incubator
24～48h of middle culture makes actication of culture, then the strain of activation is inoculated in MRS fluid medium, trains in 30～35 DEG C of constant temperature
24～48h is supported, obtains the seed liquor for activating.MRS culture medium：Casein peptone 10g, Carnis Bovis seu Bubali cream 10g, yeast powder 5g, glucose 5g,
Sodium acetate 5g, dibasic ammonium citrate 2g, Tween 80 1g, K2HPO42g, MgSO4.7H2O 0.2g, MnSO4.H2O 0.05g,
CaCO320g, distilled water 1L, pH6.8.(slant medium need to add agar 15g), with 115 DEG C of sterilizing 20min.
Rhizopus javanicus (from Wuhan University's China typical culture collection center, resource number is BNCC183863)
Activation：Rhizopus javanicus are taken, is inoculated in ready Cha Shi yeast extract agar culture medium, is positioned over 28～30 DEG C of constant temperature culture
Cultivate in case to spore is grown, have the mycelia of spore to the triangular flask equipped with sterilized water with inoculation shovel scraping length, add aseptic
Bead vibrates, and is filtered with the aseptic funnel of plug Cotton Gossypii, collects filtrate, adjustment spore liquid concentration to 106Individual/ml, obtains seed
Liquid.Cha Shi yeast extract agar culture medium：Sucrose 30g, NaNO33g, MgSO4·7H2O 0.5g, KCl 0.5g, FeSO4·4H2O
0.01g, K2HPO41g, agar 15g, distilled water 1L, pH6.0～6.5, with 115 DEG C of sterilizing 20min.
(from Wuhan University's China typical culture collection center, resource number is candida krusei
BNCC185429 activation)：Candida krusei is taken, is inoculated on ready YPD slant medium, is positioned over 30 DEG C
In constant incubator, 24～48h of culture makes actication of culture, then the strain of activation is inoculated in YPD fluid medium, in 25～
30 DEG C of 24～48h of constant temperature culture, obtain the seed liquor for activating.YPD culture medium：Glucose 20g, yeast extract 10g, peptone 20g,
Distilled water 100mL (slant medium need to add agar 15g), with 115 DEG C of sterilizing 20min.
Embodiment 1：The preparation method of a kind of fermented type Flos Caraganae Sinicae tobacco aromatics using, it is characterised in that concrete steps are such as
Under：
(1) 100g Flos Caraganae Sinicae is weighed, to 500ml triangular flask, according to raw material and water 1:2 ratio adds water, at 121 DEG C
5～10min of lower sterilizing, after being cooled to room temperature, adjusts pH value to 4.5～7.0, adds the 1% of Flos Caraganae Sinicae weight cellulase
Saccharifying enzyme with 1%, reacts 4h in temperature 50 C, the constant-temperature table of 100～200r/min of rotating speed, then adjusts pH value to 3.0
～6.0, in 40 DEG C of temperature, in the constant-temperature table of 100～200r/min of rotating speed, react 2h；
(2) in above-mentioned Flos Caraganae Sinicae enzymolysis solution add Flos Caraganae Sinicae enzymolysis solution weight 3% glucose, 0.5% ammonium sulfate,
0.3% Magnesium sulfate heptahydrate, 0.5% Sodium Chloride, 0.1% ferrous sulfate heptahydrate, sterilize at 115 DEG C 5～15min；
(3) strain for having activated is seeded in the good enzymolysis solution of pretreatment, in temperature 25～35 according to a certain amount of
DEG C, 24～72h is cultivated in the constant-temperature table of 100～200r/min of rotating speed；Inoculum concentration is：Plant lactobacillus 3%, Rhizopus javanicus
1%, candida krusei 2%；
(4) in fermented product all being added to 1000ml round-bottomed flask, adding 200ml water, is heated to 90 DEG C of backflows, extracts
1.5h, using 0.8 μm of membrane filtration, collects filtrate, is evaporated to paste, and the pressure of concentrating under reduced pressure is 0.08MPa.
Embodiment 2：The preparation method of a kind of fermented type Flos Caraganae Sinicae tobacco aromatics using, it is characterised in that concrete steps are such as
Under：
(1) 100g Flos Caraganae Sinicae is weighed, to 500ml triangular flask, according to raw material and water 1:1 ratio adds water, at 121 DEG C
Lower sterilizing 8min, after being cooled to room temperature, adjusts pH value to 4.5～7.0, add the 1.5% of Flos Caraganae Sinicae weight cellulase and
0.5% saccharifying enzyme, reacts 2h in temperature 60 C, the constant-temperature table of 100～200r/min of rotating speed, then adjusts pH value to 3.0
～6.0, in 30 DEG C of temperature, in the constant-temperature table of 100～200r/min of rotating speed, react 3h；
(2) 5% glucose, 0.8% ammonium sulfate, 0.5% 7 water sulphuric acid are added in the Flos Caraganae Sinicae enzymolysis solution in step (1)
Magnesium, 0.3% Sodium Chloride, 0.12% ferrous sulfate heptahydrate, sterilize at 115 DEG C 10min；
(3) strain for having activated is seeded in the good enzymolysis solution of pretreatment, in temperature 25～35 according to a certain amount of
DEG C, 24～72h is cultivated in the constant-temperature table of 100～200r/min of rotating speed；Inoculum concentration is：Plant lactobacillus 5%, Rhizopus javanicus
1%, candida krusei 3%；
(4) in fermented product all being added to 1000ml round-bottomed flask, adding 300ml water, is heated to 95 DEG C of backflows, extracts
2h, using 0.8 μm of membrane filtration, collects filtrate, is evaporated to paste, and the pressure of concentrating under reduced pressure is 0.08MPa.
Test 1, Flos Caraganae Sinicae tobacco aromatics using analysis of volatile components：
(1) chromatographic condition：Using Gas chromatographyMass spectrometry (Gas chromatography-mass
Spectrometry, GC/MS) extract is analyzed；Chromatographic column is HP-5MS, and specification is 50cm × 0.25mm × 0.25 μ
m；Carrier gas is nitrogen, air inflow 1.0mL/min；260 DEG C of injector temperature；Programming rate 50 DEG C/min, 5min are raised to 250 DEG C；
1 μ L of sample size, does not shunt；IE ion source, 70eV, 50～650amu of sweep limitss, 250 DEG C of transmission line temperature；Using WILEY and
MAINLIB library searching.
(2) Flos Caraganae Sinicae tobacco aromatics using pre-treatment：The Flos Caraganae Sinicae tobacco aromatics using extract that 0.1g implements to prepare in 1 is taken, is added
10ml dehydrated alcohol so as to fully dissolve, stands 2h, takes supernatant, is filtered using the microfilter of 0.4um, and filtrate is placed
It is analyzed in GC/MS automatic sampler, concrete composition analysis result such as table 1：
The composition analysis result of the Flos Caraganae Sinicae tobacco aromatics using for preparing in 1 implemented by table 1
During the Flos Caraganae Sinicae tobacco aromatics using that present invention preparation be can be seen that by mentioned component analytical table includes respectively to grow tobacco
Aroma component such as alcohols, aldehydes, ketone, acids, esters, phenols, furans, Pyrazine, pyridines and terpenes etc..
Test 2, Flos Caraganae Sinicae tobacco aromatics using application effect appraisal：
The pure tobacco shred 10g several pieces that 22 DEG C ± 1 DEG C of temperature and relative humidity (RH) have been balanced for 60% ± 2% time are taken at,
The Flos Caraganae Sinicae tobacco aromatics using that in embodiment 1 prepare is weighed respectively by the 0.1% of its quality, 0.2%, 0.5%, with appropriate distillation
After water dilution, uniformly it is sprayed on tobacco shred with larynx jet pipe, the tobacco sample of perfuming is put into 22 DEG C ± 1 DEG C of temperature and relative humidity
(RH) 48h being balanced in 60% ± 2% climatic chamber, respectively the tobacco shred of perfuming is made cigarette with cigarette device is filled out, then in temperature
Balance 48h in the climatic chamber of 22 DEG C ± 1 DEG C and relative humidity (RH) 60% ± 2%, please specialty Medicated cigarette sensory evaluating smoking little
Group is smoked panel test, Cigarette evaluation effect such as table 2：
Table 2 be in embodiment 1 Flos Caraganae Sinicae tobacco aromatics using according to not on year-on-year basis row be added to smoking result in Medicated cigarette
The Flos Caraganae Sinicae tobacco aromatics using for preparing in the present invention can be seen that by above-mentioned evaluation result and be added to cigarette shreds
In, can reduce cigarette foreign gas, increase cigarette flavor, flue gas is more in sucking process to add after Flos Caraganae Sinicae tobacco aromatics using Medicated cigarette
Soft, smooth, zest reduces, and pleasant impression is relatively comfortable, and oral cavity is cleaner, noresidue.
Claims (5)
1. a kind of preparation method of fermented type Flos Caraganae Sinicae tobacco aromatics using, it is characterised in that comprise the following steps that：
(1) Flos Caraganae Sinicae is taken, according to raw material and water 1:1～1:3 ratio adds water, and sterilize at 121 DEG C 5～10min, is cooled to
After room temperature, pH value is adjusted to 4.5～7.0, add the cellulase of Flos Caraganae Sinicae weight 0.5～1.5% and 0.5～1.5% sugar
Change enzyme, react 2～5h in the constant-temperature table of 40～60 DEG C of temperature, then pH value is adjusted to 3.0～6.0, in 30～55 DEG C of temperature
Constant-temperature table in react 1～3h；
(2) 1 is added according to the mass percent of Flos Caraganae Sinicae enzymolysis solution in the Flos Caraganae Sinicae enzymolysis solution after reaction in step (1)～
5% glucose, 0.1～1% ammonium sulfate, 0.1～0.5% Magnesium sulfate heptahydrate, 0.1～1% Sodium Chloride, 0.05～0.12% 7 water
Ferrous sulfate, then at 115 DEG C, 5～15min of sterilizing obtains the Flos Caraganae Sinicae enzymolysis solution of pretreatment；
(3) activation of fermented bacterium：
A. (from Wuhan University's China typical culture collection center, resource number is plant lactobacillus
Activation：Plant lactobacillus are taken, is inoculated on ready MRS slant medium, is positioned in 30～35 DEG C of constant incubator
24～48h of culture makes actication of culture, then the strain of activation is inoculated in MRS fluid medium, in 30～35 DEG C of constant temperature culture
24～48h, obtains the plant lactobacillus seed liquor for activating；
B. the work of Rhizopus javanicus (from Wuhan University's China typical culture collection center, resource number is BNCC183863)
Change：Rhizopus javanicus are taken, is inoculated in ready Cha Shi yeast extract agar culture medium, is positioned over 28～30 DEG C of constant incubator
In cultivate to growing spore, have the mycelia of spore to the triangular flask equipped with sterilized water with inoculation shovel scraping length, add aseptic glass
Glass pearl is vibrated, and is filtered with the aseptic funnel of plug Cotton Gossypii, collects filtrate, adjustment spore liquid concentration to 106Individual/ml, obtains Java root
Mould seed liquor；
C. (from Wuhan University's China typical culture collection center, resource number is candida krusei
BNCC185429 activation)：Candida krusei is taken, is inoculated on ready YPD slant medium, is positioned over 30 DEG C
In constant incubator, 24～48h of culture makes actication of culture, then the strain of activation is inoculated in YPD fluid medium, in 25～
30 DEG C of 24～48h of constant temperature culture, obtain the candida krusei seed liquor for activating；
(4). the strain for having activated in step (3) is seeded to, according to a certain amount of, the Flos Caraganae Sinicae enzymolysis that in step (2), pretreatment is good
In liquid, in 25～35 DEG C of temperature, in the constant-temperature table of 100～200r/min of rotating speed, 24～72h of culture carries out fermentation reaction；Described
The inoculum concentration that all plants according to the mass percent of Flos Caraganae Sinicae enzymolysis solution is：Plant lactobacillus seed liquor 1～5%, Rhizopus javanicus kind
Sub- liquid 0.5～2%, candida krusei seed liquor 1～3%；
(5). the whole fermented products after fermentation in step (4) are taken, then according to fermented product compares 1 with the weight of water:0.5-1:2 ratio
Example adds water, is heated to 85 DEG C～95 DEG C 1～2h of reflux, extract, using 0.8 μm of membrane filtration, collects filtrate, concentrating under reduced pressure
Flos Caraganae Sinicae tobacco aromatics using to paste.
2. a kind of preparation method of fermented type Flos Caraganae Sinicae tobacco aromatics using according to claim 1, it is characterised in that the step
Suddenly in (1), the constant-temperature table rotating speed of enzyme digestion reaction is 100～200r/min twice.
3. a kind of preparation method of fermented type Flos Caraganae Sinicae tobacco aromatics using according to claim 1, it is characterised in that the step
Suddenly in (3), the formula of the MRS fluid medium of activation plant lactobacillus is as follows：10g casein peptone, 10g Carnis Bovis seu Bubali cream, 5g yeast
Powder, 5g glucose, 5g sodium acetate, 2g dibasic ammonium citrate, the K of Tween 80, the 2g of 1g2HPO4, the MgSO of 0.2g4.7H2O,
The MnSO of 0.05g4.H2The CaCO of O, 20g3, 1L distilled water, pH6.8；The MRS slant medium be in MRS fluid medium
Formula in add 15g agar；The MRS fluid medium and MRS slant medium are all gone out after preparing at 115 DEG C
Bacterium 20min.
4. a kind of preparation method of fermented type Flos Caraganae Sinicae tobacco aromatics using according to claim 1, it is characterised in that the step
Suddenly in (3), the formula of the Cha Shi yeast extract agar culture medium of activation Rhizopus javanicus is as follows：30g sucrose, the NaNO of 3g3, 0.5g's
MgSO4·7H2The FeSO of KCl, the 0.01g of O, 0.5g4·4H2The K of O, 1g2HPO4, 15g agar, 1L distilled water, pH6.0～
6.5, the culture medium is with 115 DEG C of sterilizing 20min after preparing.
5. a kind of preparation method of fermented type Flos Caraganae Sinicae tobacco aromatics using according to claim 1, it is characterised in that the step
Suddenly in (3), the formula of the YPD culture medium of candida krusei is as follows：Glucose 20g, yeast extract 10g, peptone 20g, distillation
Water 100mL；The YPD slant medium is interpolation 15g agar in YPD culture medium prescription；The YPD culture medium and YPD are oblique
Face culture medium is all with 115 DEG C of sterilizing 20min after preparing.
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